gold conjugated goat anti mouse antibody  (Thermo Fisher)


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    Name:
    F ab 2 Goat anti Mouse IgM mu Functional Grade Secondary Antibody
    Description:
    F ab 2 Goat anti Mouse IgM mu Functional Grade Secondary Antibody for Flow FN
    Catalog Number:
    16-5092-85
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher gold conjugated goat anti mouse antibody
    Localization of NADPH oxidase proteins in rabbit aorta. Aortic sections were incubated with control nonimmune <t>mouse</t> serum ( A ) or monoclonal antibodies against p22 phox (1:100) ( B and C ), gp91 phox (1:200) ( D ), p47 phox (1:20) ( E ), and p67 phox (1:20) ( F ) at the dilutions shown, followed by <t>gold-conjugated</t> <t>goat</t> <t>anti-mouse</t> antibody. The sections were developed as described in Methods . Magnification is 190× ( A and C–F ) and 95× ( B ). The sections shown represent at least three stained sections for each antibody.
    F ab 2 Goat anti Mouse IgM mu Functional Grade Secondary Antibody for Flow FN
    https://www.bioz.com/result/gold conjugated goat anti mouse antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gold conjugated goat anti mouse antibody - by Bioz Stars, 2021-06
    97/100 stars

    Images

    1) Product Images from "Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: Enhancement by angiotensin II"

    Article Title: Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: Enhancement by angiotensin II

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi:

    Localization of NADPH oxidase proteins in rabbit aorta. Aortic sections were incubated with control nonimmune mouse serum ( A ) or monoclonal antibodies against p22 phox (1:100) ( B and C ), gp91 phox (1:200) ( D ), p47 phox (1:20) ( E ), and p67 phox (1:20) ( F ) at the dilutions shown, followed by gold-conjugated goat anti-mouse antibody. The sections were developed as described in Methods . Magnification is 190× ( A and C–F ) and 95× ( B ). The sections shown represent at least three stained sections for each antibody.
    Figure Legend Snippet: Localization of NADPH oxidase proteins in rabbit aorta. Aortic sections were incubated with control nonimmune mouse serum ( A ) or monoclonal antibodies against p22 phox (1:100) ( B and C ), gp91 phox (1:200) ( D ), p47 phox (1:20) ( E ), and p67 phox (1:20) ( F ) at the dilutions shown, followed by gold-conjugated goat anti-mouse antibody. The sections were developed as described in Methods . Magnification is 190× ( A and C–F ) and 95× ( B ). The sections shown represent at least three stained sections for each antibody.

    Techniques Used: Incubation, Staining

    Related Articles

    Mouse Assay:

    Article Title: Induction of Colonic M Cells during Intestinal Inflammation
    Article Snippet: Mice were given either DSS in their drinking water or control water. .. Mice were then given three i.p. injections containing 100 μg of a functional-grade anti-mouse TNF-α (eBioscience, San Diego, CA) at days D 0, 3, and 5 of DSS treatment. .. These mice were also assessed daily for signs of colonic distress.

    Functional Assay:

    Article Title: Induction of Colonic M Cells during Intestinal Inflammation
    Article Snippet: Mice were given either DSS in their drinking water or control water. .. Mice were then given three i.p. injections containing 100 μg of a functional-grade anti-mouse TNF-α (eBioscience, San Diego, CA) at days D 0, 3, and 5 of DSS treatment. .. These mice were also assessed daily for signs of colonic distress.

    Staining:

    Article Title: Mucolipin-2 Cation Channel Increases Trafficking Efficiency of Endocytosed Viruses
    Article Snippet: .. Samples were subsequently stained with goat anti-mouse conjugated to AF488 (Invitrogen), and cell fluorescence was quantified by flow cytometry. .. An S1000 flow cytometer (Stratedigm) was used, and data were quantified using FlowJo.

    Article Title: The peptide symporter SLC15a4 is essential for the development of systemic lupus erythematosus in murine models
    Article Snippet: Isotype control antibodies were used to confirm antibody specificity for all immunohistochemical stained tissues. .. Renal immune complex immunofluorescence and quantificationGlomerular immune complex deposits were visualized on acetone-fixed 5-micron OCT embedded kidney sections by direct immunofluorescence staining using Alexa Fluor 488-conjugated donkey anti-mu-IgG or goat anti-mu-IgM (Invitrogen); isotype control antibodies were used to confirm antibody specificity for all immunofluorescence stained tissues. .. Slides were mounted with ProLong Gold Antifade (Invitrogen, Thermo Fisher Scientific), then imaged on a NanoZoomer XR automated slide scanning platform (Hamamatsu) at 200x final magnification.

    Fluorescence:

    Article Title: Mucolipin-2 Cation Channel Increases Trafficking Efficiency of Endocytosed Viruses
    Article Snippet: .. Samples were subsequently stained with goat anti-mouse conjugated to AF488 (Invitrogen), and cell fluorescence was quantified by flow cytometry. .. An S1000 flow cytometer (Stratedigm) was used, and data were quantified using FlowJo.

    Flow Cytometry:

    Article Title: Mucolipin-2 Cation Channel Increases Trafficking Efficiency of Endocytosed Viruses
    Article Snippet: .. Samples were subsequently stained with goat anti-mouse conjugated to AF488 (Invitrogen), and cell fluorescence was quantified by flow cytometry. .. An S1000 flow cytometer (Stratedigm) was used, and data were quantified using FlowJo.

    Cytometry:

    Article Title: Mucolipin-2 Cation Channel Increases Trafficking Efficiency of Endocytosed Viruses
    Article Snippet: .. Samples were subsequently stained with goat anti-mouse conjugated to AF488 (Invitrogen), and cell fluorescence was quantified by flow cytometry. .. An S1000 flow cytometer (Stratedigm) was used, and data were quantified using FlowJo.

    Negative Control:

    Article Title: Mutational Evidence for Control of Cell Adhesion Through Integrin Diffusion/Clustering, Independent of Ligand Binding
    Article Snippet: K562 transfectants were maintained in RPMI-1640 containing 10% fetal bovine serum (FBS), 1 mg/ml G418 sulfate ( GIBCO BRL , Gaithersburg, MD), and antibiotics, whereas CHO transfectants were maintained in MEMα− media containing 10% dialyzed FBS, 0.5 mg/ml G418 sulfate, and antibiotics. .. The antibodies used in this study include anti-α4 , B5G10 , and A4-PUJ1 ( ); anti-CD32 (anti-FcγRII), 4.6.19 ( ); fluorescein-conjugated goat anti–mouse IgG (Cappel, Westchester, PA); fluorescein-conjugated goat anti– mouse κ (Caltag, San Francisco, CA); negative control mAb J-2A2 ( ); and mAb 15/7, recognizing a β1 epitope induced by manganese or ligand ( ). .. Fluoresceinated B5G10 was produced using N -hydroxy succinimide (NHS)–fluorescein ( Pierce , Rockford, IL), as described by the manufacturer.

    Incubation:

    Article Title: Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: Enhancement by angiotensin II
    Article Snippet: The sections were then incubated overnight with control nonimmune mouse serum or with previously characterized monoclonal antibodies specifically recognizing gp91phox , p22phox , p47phox , and p67phox ( ) in DPBS containing 1% BSA, 0.1% Tween 20, 0.1% NaN3 , and 1% goat serum. .. After washing with blotto, the sections were incubated for 60 min at 25°C with 5 nm gold-conjugated goat anti-mouse antibody (Zymed) diluted 1:50 in the same buffer. .. The sections were rinsed with H2 O, developed using silver acetate enhancing in the dark , and counterstained by using nuclear fast red (Vector Laboratories).

    Immunofluorescence:

    Article Title: The peptide symporter SLC15a4 is essential for the development of systemic lupus erythematosus in murine models
    Article Snippet: Isotype control antibodies were used to confirm antibody specificity for all immunohistochemical stained tissues. .. Renal immune complex immunofluorescence and quantificationGlomerular immune complex deposits were visualized on acetone-fixed 5-micron OCT embedded kidney sections by direct immunofluorescence staining using Alexa Fluor 488-conjugated donkey anti-mu-IgG or goat anti-mu-IgM (Invitrogen); isotype control antibodies were used to confirm antibody specificity for all immunofluorescence stained tissues. .. Slides were mounted with ProLong Gold Antifade (Invitrogen, Thermo Fisher Scientific), then imaged on a NanoZoomer XR automated slide scanning platform (Hamamatsu) at 200x final magnification.

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    Thermo Fisher gold conjugated goat anti mouse antibody
    Localization of NADPH oxidase proteins in rabbit aorta. Aortic sections were incubated with control nonimmune <t>mouse</t> serum ( A ) or monoclonal antibodies against p22 phox (1:100) ( B and C ), gp91 phox (1:200) ( D ), p47 phox (1:20) ( E ), and p67 phox (1:20) ( F ) at the dilutions shown, followed by <t>gold-conjugated</t> <t>goat</t> <t>anti-mouse</t> antibody. The sections were developed as described in Methods . Magnification is 190× ( A and C–F ) and 95× ( B ). The sections shown represent at least three stained sections for each antibody.
    Gold Conjugated Goat Anti Mouse Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gold conjugated goat anti mouse antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gold conjugated goat anti mouse antibody - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    86
    thermo fisher gold nanoparticles conjugated anti mouse goat antibody
    Correlative light and electron microscopy (CLEM) imaging of damaged AnxA6-GFP-expressing LHCN myotubes. LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed on the edge ( A ) or on the surface ( C , E ) of the myotubes as featured in ( B ) and ( D ), respectively. After membrane injury, AnxA6-GFP (green) was observed in fluorescence microscopy for about two minutes and chemically fixed. Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold <t>nanoparticle</t> (black particles in A and E ) and analyzed by TEM (bright images in A and E ). Fluorescence images in ( A ) and ( C ) correspond to the localization of AnxA6-GFP about two minutes after membrane injury. In ( E ), the diagram on the right represents the irradiated area and the location of the three sections observed. Red arrow, irradiated area; red dotted lines, boundaries of the damaged area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.
    Gold Nanoparticles Conjugated Anti Mouse Goat Antibody, supplied by thermo fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gold nanoparticles conjugated anti mouse goat antibody/product/thermo fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gold nanoparticles conjugated anti mouse goat antibody - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher colloidal gold 10 nm particle diameter conjugated goat anti mouse immunoglobulin g
    Correlative light and electron microscopy (CLEM) imaging of damaged AnxA6-GFP-expressing LHCN myotubes. LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed on the edge ( A ) or on the surface ( C , E ) of the myotubes as featured in ( B ) and ( D ), respectively. After membrane injury, AnxA6-GFP (green) was observed in fluorescence microscopy for about two minutes and chemically fixed. Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold <t>nanoparticle</t> (black particles in A and E ) and analyzed by TEM (bright images in A and E ). Fluorescence images in ( A ) and ( C ) correspond to the localization of AnxA6-GFP about two minutes after membrane injury. In ( E ), the diagram on the right represents the irradiated area and the location of the three sections observed. Red arrow, irradiated area; red dotted lines, boundaries of the damaged area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.
    Colloidal Gold 10 Nm Particle Diameter Conjugated Goat Anti Mouse Immunoglobulin G, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/colloidal gold 10 nm particle diameter conjugated goat anti mouse immunoglobulin g/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    colloidal gold 10 nm particle diameter conjugated goat anti mouse immunoglobulin g - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    98
    Thermo Fisher 10 nm gold goat anti mouse conjugate
    Correlative light and electron microscopy (CLEM) imaging of damaged AnxA6-GFP-expressing LHCN myotubes. LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed on the edge ( A ) or on the surface ( C , E ) of the myotubes as featured in ( B ) and ( D ), respectively. After membrane injury, AnxA6-GFP (green) was observed in fluorescence microscopy for about two minutes and chemically fixed. Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold <t>nanoparticle</t> (black particles in A and E ) and analyzed by TEM (bright images in A and E ). Fluorescence images in ( A ) and ( C ) correspond to the localization of AnxA6-GFP about two minutes after membrane injury. In ( E ), the diagram on the right represents the irradiated area and the location of the three sections observed. Red arrow, irradiated area; red dotted lines, boundaries of the damaged area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.
    10 Nm Gold Goat Anti Mouse Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10 nm gold goat anti mouse conjugate/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10 nm gold goat anti mouse conjugate - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    Image Search Results


    Localization of NADPH oxidase proteins in rabbit aorta. Aortic sections were incubated with control nonimmune mouse serum ( A ) or monoclonal antibodies against p22 phox (1:100) ( B and C ), gp91 phox (1:200) ( D ), p47 phox (1:20) ( E ), and p67 phox (1:20) ( F ) at the dilutions shown, followed by gold-conjugated goat anti-mouse antibody. The sections were developed as described in Methods . Magnification is 190× ( A and C–F ) and 95× ( B ). The sections shown represent at least three stained sections for each antibody.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Localization of a constitutively active, phagocyte-like NADPH oxidase in rabbit aortic adventitia: Enhancement by angiotensin II

    doi:

    Figure Lengend Snippet: Localization of NADPH oxidase proteins in rabbit aorta. Aortic sections were incubated with control nonimmune mouse serum ( A ) or monoclonal antibodies against p22 phox (1:100) ( B and C ), gp91 phox (1:200) ( D ), p47 phox (1:20) ( E ), and p67 phox (1:20) ( F ) at the dilutions shown, followed by gold-conjugated goat anti-mouse antibody. The sections were developed as described in Methods . Magnification is 190× ( A and C–F ) and 95× ( B ). The sections shown represent at least three stained sections for each antibody.

    Article Snippet: After washing with blotto, the sections were incubated for 60 min at 25°C with 5 nm gold-conjugated goat anti-mouse antibody (Zymed) diluted 1:50 in the same buffer.

    Techniques: Incubation, Staining

    Correlative light and electron microscopy (CLEM) imaging of damaged AnxA6-GFP-expressing LHCN myotubes. LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed on the edge ( A ) or on the surface ( C , E ) of the myotubes as featured in ( B ) and ( D ), respectively. After membrane injury, AnxA6-GFP (green) was observed in fluorescence microscopy for about two minutes and chemically fixed. Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold nanoparticle (black particles in A and E ) and analyzed by TEM (bright images in A and E ). Fluorescence images in ( A ) and ( C ) correspond to the localization of AnxA6-GFP about two minutes after membrane injury. In ( E ), the diagram on the right represents the irradiated area and the location of the three sections observed. Red arrow, irradiated area; red dotted lines, boundaries of the damaged area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.

    Journal: Cells

    Article Title: Annexin-A6 in Membrane Repair of Human Skeletal Muscle Cell: A Role in the Cap Subdomain

    doi: 10.3390/cells9071742

    Figure Lengend Snippet: Correlative light and electron microscopy (CLEM) imaging of damaged AnxA6-GFP-expressing LHCN myotubes. LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed on the edge ( A ) or on the surface ( C , E ) of the myotubes as featured in ( B ) and ( D ), respectively. After membrane injury, AnxA6-GFP (green) was observed in fluorescence microscopy for about two minutes and chemically fixed. Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold nanoparticle (black particles in A and E ) and analyzed by TEM (bright images in A and E ). Fluorescence images in ( A ) and ( C ) correspond to the localization of AnxA6-GFP about two minutes after membrane injury. In ( E ), the diagram on the right represents the irradiated area and the location of the three sections observed. Red arrow, irradiated area; red dotted lines, boundaries of the damaged area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.

    Article Snippet: Mouse anti-AnxA6 monoclonal antibody at 1:100 and secondary Alexa Fluor 488- and gold nanoparticles-conjugated anti-mouse goat antibody (FluoroNanogold, Nanoprobes, NY, USA) at 1:100 were successively incubated with myotubes for 1 h at 37 °C.

    Techniques: Electron Microscopy, Imaging, Expressing, Transfection, Plasmid Preparation, Fluorescence, Microscopy, Transmission Electron Microscopy, Irradiation

    CLEM imaging of AnxA6-GFP-expressing LHCN myotubes during the formation of the cap subdomain. ( A ) LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed at the edge of the myotubes, which were then rapidly fixed. ( B ) Recruitment of AnxA6-GFP was checked by fluorescence microscopy as described in the legend of Figure 6 . ( C ) Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold nanoparticle and observed in TEM. Red and white arrows, irradiated area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.

    Journal: Cells

    Article Title: Annexin-A6 in Membrane Repair of Human Skeletal Muscle Cell: A Role in the Cap Subdomain

    doi: 10.3390/cells9071742

    Figure Lengend Snippet: CLEM imaging of AnxA6-GFP-expressing LHCN myotubes during the formation of the cap subdomain. ( A ) LHCN myotubes were transfected with pA6-GFP plasmid and membrane damage was performed at the edge of the myotubes, which were then rapidly fixed. ( B ) Recruitment of AnxA6-GFP was checked by fluorescence microscopy as described in the legend of Figure 6 . ( C ) Myotubes were then immunostained for AnxA6 with a secondary antibody coupled with a gold nanoparticle and observed in TEM. Red and white arrows, irradiated area. Scale bar in fluorescence microscopy: 5 µm. Scale bar in TEM: 1 µm.

    Article Snippet: Mouse anti-AnxA6 monoclonal antibody at 1:100 and secondary Alexa Fluor 488- and gold nanoparticles-conjugated anti-mouse goat antibody (FluoroNanogold, Nanoprobes, NY, USA) at 1:100 were successively incubated with myotubes for 1 h at 37 °C.

    Techniques: Imaging, Expressing, Transfection, Plasmid Preparation, Fluorescence, Microscopy, Transmission Electron Microscopy, Irradiation