goat immunoglobulin g igg  (Agilent technologies)

 
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    Name:
    Multiple Affinity Removal Spin Cartridge HSA IgG
    Description:
    The Multiple Affinity Removal Spin Cartridge Human Albumin IgG is designed to remove two interfering high abundance proteins from human samples e g plasma serum urine or cerebrospinal fluid They are compatible with standard benchtop microcentrifuges and luer lok syringes Removal of these abundant proteins improves subsequent LC MS and electrophoretic analysis of samples by effectively expanding the dynamic range of the analysis
    Catalog Number:
    5188-8825
    Price:
    None
    Category:
    Products Biopharma Hplc Analysis Abundant Protein Depletion Multiple Affinity Removal Spin Cartridge Hsa Igg
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    Structured Review

    Agilent technologies goat immunoglobulin g igg
    The Multiple Affinity Removal Spin Cartridge Human Albumin IgG is designed to remove two interfering high abundance proteins from human samples e g plasma serum urine or cerebrospinal fluid They are compatible with standard benchtop microcentrifuges and luer lok syringes Removal of these abundant proteins improves subsequent LC MS and electrophoretic analysis of samples by effectively expanding the dynamic range of the analysis
    https://www.bioz.com/result/goat immunoglobulin g igg/product/Agilent technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat immunoglobulin g igg - by Bioz Stars, 2021-04
    99/100 stars

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    Filtration:

    Article Title: Exosomal Secretion of Cytoplasmic Prostate Cancer Xenograft-derived Proteins *Exosomal Secretion of Cytoplasmic Prostate Cancer Xenograft-derived Proteins * S⃞
    Article Snippet: The protocol was approved by the Animal Experiments Committee under the national Experiments on Animals Act and adhered to the rules laid down in this national law that serves the implementation of “Guidelines on the protection of experimental animals” by the council of Europe under Directive 86/609/EC. .. After filtration using a 0.22-μm spin filter, high abundance proteins were removed utilizing Multi Affinity Removal Spin cartridges (Agilent Technologies, Wilmington, DE) according to the manufacturer's instructions. .. Depleted samples were concentrated on 5-kDa-cutoff ultracentrifugation columns (Agilent Technologies).

    other:

    Article Title: Aberrant sialylation of a prostate-specific antigen: Electrochemical label-free glycoprofiling in prostate cancer serum samples
    Article Snippet: Before electrochemical analysis, human serum samples (50 μL) were depleted from IgG and HAS according to the manufacturer's instructions (Multiple Affinity Removal Spin Cartridge HSA/IgG, Agilent Technologies, CA, USA) and prepared in 10 mM PBS buffer, pH 7.4.

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    Agilent technologies biotinylated goat anti mouse secondary antibody
    Scheme of enzyme-enhanced LFIC for VCP detection <t>Biotinylated</t> gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.
    Biotinylated Goat Anti Mouse Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse secondary antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti mouse secondary antibody - by Bioz Stars, 2021-04
    86/100 stars
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    86
    Agilent technologies polyclonal peroxidase conjugated goat anti mouse igg antibody
    The 2-D reference gels of F. tularensis FSC200 whole-cell lysate protein targets, which are recognized by sera from F. tularensis FSC200 infected Balb/c GF mice. ( a ) The targets of Balb/c GF mice antibody response at 12 h post infection, ( b ) the depicted unique targets of Balb/c GF mice antibody response at 24 and 48 h post infection, ( c ) the depicted unique antibody response of Balb/c SPF mice at 24 and 48 h post infection. A total 150 μg of protein was separated by immobilized pH gradient (IPG) strips (3–10) and 12% (w/v) SDS-PAGE gels. Identified immunogenic protein spots were numbered in preparative 2-D SDS-PAGE gels, excised for MS/MS analysis, corresponding to the proteins in Table S1 (designated letters and numbers of protein spots were used only for internal purposes and do not necessarily imply the same identity of the labeled proteins). <t>Polyclonal</t> peroxidase-conjugated goat anti-mouse <t>IgG</t> antibody was used for secondary antibody detection. All experiments were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times.
    Polyclonal Peroxidase Conjugated Goat Anti Mouse Igg Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal peroxidase conjugated goat anti mouse igg antibody/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
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    86
    Agilent technologies fitc labelled goat anti mouse igg
    Ca2+-dependent interaction between mannose-binding lectin (MBL) and C. parapsilosis and reference strains C. albicans , C. neoformans , S. aureus and E. coli . Microorganisms were incubated in the presence of 5 μg/mL purified human MBL in VSB 2+ . After incubation with mouse anti-MBL monoclonal antibody and <t>FITC-labeled</t> goat anti mouse <t>IgG,</t> binding of MBL to these microorganisms was analyzed by flow cytometry. Results are expressed as median fluorescence intensity (MFI). Data are the mean ± SEM of 3 separate experiments. Solid bars, addition of human purified MBL; open bars, no addition of human purified MBL.
    Fitc Labelled Goat Anti Mouse Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc labelled goat anti mouse igg/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    86
    Agilent technologies antibody horseradish peroxidase labeled polymer conjugated goat anti rabbit immunoglobulin g
    Immunohistochemistry showing the localization of DUSP9 protein in placental tissues from mothers with gestational diabetes mellitus (GDM) or with normal pregnancies. As negative controls, tissue sections were incubated in parallel with isotype <t>IgG</t> at the same concentration as the primary antibody or with only secondary antibody without primary antibody. DUSP9 was observed in the cytotrophoblasts of both groups (arrows). Magnification: 200x (upper row) or 400x (lower row).
    Antibody Horseradish Peroxidase Labeled Polymer Conjugated Goat Anti Rabbit Immunoglobulin G, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody horseradish peroxidase labeled polymer conjugated goat anti rabbit immunoglobulin g/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody horseradish peroxidase labeled polymer conjugated goat anti rabbit immunoglobulin g - by Bioz Stars, 2021-04
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    Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

    Journal: Oncotarget

    Article Title: Point-of-care test for cervical cancer in LMICs

    doi: 10.18632/oncotarget.7709

    Figure Lengend Snippet: Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

    Article Snippet: This was followed by the application of biotinylated goat anti-mouse secondary antibody (GAM IgG) conjugated to HRP (Dakocytomation, Carpinteria, CA).

    Techniques: Flow Cytometry, Stripping Membranes

    The 2-D reference gels of F. tularensis FSC200 whole-cell lysate protein targets, which are recognized by sera from F. tularensis FSC200 infected Balb/c GF mice. ( a ) The targets of Balb/c GF mice antibody response at 12 h post infection, ( b ) the depicted unique targets of Balb/c GF mice antibody response at 24 and 48 h post infection, ( c ) the depicted unique antibody response of Balb/c SPF mice at 24 and 48 h post infection. A total 150 μg of protein was separated by immobilized pH gradient (IPG) strips (3–10) and 12% (w/v) SDS-PAGE gels. Identified immunogenic protein spots were numbered in preparative 2-D SDS-PAGE gels, excised for MS/MS analysis, corresponding to the proteins in Table S1 (designated letters and numbers of protein spots were used only for internal purposes and do not necessarily imply the same identity of the labeled proteins). Polyclonal peroxidase-conjugated goat anti-mouse IgG antibody was used for secondary antibody detection. All experiments were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times.

    Journal: Scientific Reports

    Article Title: Early infection-induced natural antibody response

    doi: 10.1038/s41598-021-81083-0

    Figure Lengend Snippet: The 2-D reference gels of F. tularensis FSC200 whole-cell lysate protein targets, which are recognized by sera from F. tularensis FSC200 infected Balb/c GF mice. ( a ) The targets of Balb/c GF mice antibody response at 12 h post infection, ( b ) the depicted unique targets of Balb/c GF mice antibody response at 24 and 48 h post infection, ( c ) the depicted unique antibody response of Balb/c SPF mice at 24 and 48 h post infection. A total 150 μg of protein was separated by immobilized pH gradient (IPG) strips (3–10) and 12% (w/v) SDS-PAGE gels. Identified immunogenic protein spots were numbered in preparative 2-D SDS-PAGE gels, excised for MS/MS analysis, corresponding to the proteins in Table S1 (designated letters and numbers of protein spots were used only for internal purposes and do not necessarily imply the same identity of the labeled proteins). Polyclonal peroxidase-conjugated goat anti-mouse IgG antibody was used for secondary antibody detection. All experiments were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times.

    Article Snippet: Polyclonal peroxidase-conjugated goat anti-mouse IgG antibody (Dako, Copenhagen, Denmark) or monoclonal HRP-conjugated goat anti-mouse IgM antibody (Invitrogen, Carlsbad, Ca, USA) was diluted 1:100 in blocking buffer and used for secondary antibody detection.

    Techniques: Infection, Mouse Assay, SDS Page, Tandem Mass Spectroscopy, Labeling

    Western blot analysis to evaluate F. tularensis FSC200 whole-cell lysate protein targets recognized by sera from F. tularensis FSC200 infected Balb/c mice and detected by chemiluminescence. The 2-D reference immunoblots demonstrated the reaction of antibody clones in murine sera obtained from Balb/c GF (upper) and SPF (bottom) mice at 12 h post infection. A total 200 μg of protein was separated by immobilized pH gradient (IPG) strips (3–10) and 12% (w/v) SDS-PAGE gels. Polyclonal peroxidase-conjugated goat anti-mouse IgG antibody was used for secondary antibody detection. All experiments were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times.

    Journal: Scientific Reports

    Article Title: Early infection-induced natural antibody response

    doi: 10.1038/s41598-021-81083-0

    Figure Lengend Snippet: Western blot analysis to evaluate F. tularensis FSC200 whole-cell lysate protein targets recognized by sera from F. tularensis FSC200 infected Balb/c mice and detected by chemiluminescence. The 2-D reference immunoblots demonstrated the reaction of antibody clones in murine sera obtained from Balb/c GF (upper) and SPF (bottom) mice at 12 h post infection. A total 200 μg of protein was separated by immobilized pH gradient (IPG) strips (3–10) and 12% (w/v) SDS-PAGE gels. Polyclonal peroxidase-conjugated goat anti-mouse IgG antibody was used for secondary antibody detection. All experiments were performed in biological triplicates (individual sera) for all time intervals and were independently repeated at least three times.

    Article Snippet: Polyclonal peroxidase-conjugated goat anti-mouse IgG antibody (Dako, Copenhagen, Denmark) or monoclonal HRP-conjugated goat anti-mouse IgM antibody (Invitrogen, Carlsbad, Ca, USA) was diluted 1:100 in blocking buffer and used for secondary antibody detection.

    Techniques: Western Blot, Infection, Mouse Assay, Clone Assay, SDS Page

    Ca2+-dependent interaction between mannose-binding lectin (MBL) and C. parapsilosis and reference strains C. albicans , C. neoformans , S. aureus and E. coli . Microorganisms were incubated in the presence of 5 μg/mL purified human MBL in VSB 2+ . After incubation with mouse anti-MBL monoclonal antibody and FITC-labeled goat anti mouse IgG, binding of MBL to these microorganisms was analyzed by flow cytometry. Results are expressed as median fluorescence intensity (MFI). Data are the mean ± SEM of 3 separate experiments. Solid bars, addition of human purified MBL; open bars, no addition of human purified MBL.

    Journal: BMC Microbiology

    Article Title: Mannose binding lectin plays a crucial role in innate immunity against yeast by enhanced complement activation and enhanced uptake of polymorphonuclear cells

    doi: 10.1186/1471-2180-8-229

    Figure Lengend Snippet: Ca2+-dependent interaction between mannose-binding lectin (MBL) and C. parapsilosis and reference strains C. albicans , C. neoformans , S. aureus and E. coli . Microorganisms were incubated in the presence of 5 μg/mL purified human MBL in VSB 2+ . After incubation with mouse anti-MBL monoclonal antibody and FITC-labeled goat anti mouse IgG, binding of MBL to these microorganisms was analyzed by flow cytometry. Results are expressed as median fluorescence intensity (MFI). Data are the mean ± SEM of 3 separate experiments. Solid bars, addition of human purified MBL; open bars, no addition of human purified MBL.

    Article Snippet: After a 30-min incubation on ice, the samples were centrifuged and washed as described above and the pellets were resuspended in FITC-labelled goat anti-mouse IgG (DakoCytomation, Glostrup, Denmark) (80 μg/mL in PBS) and were incubated on ice for 30 min. Suspensions were centrifuged and washed as described above and measured by flow cytometry.

    Techniques: Binding Assay, Incubation, Purification, Labeling, Flow Cytometry, Cytometry, Fluorescence

    Immunohistochemistry showing the localization of DUSP9 protein in placental tissues from mothers with gestational diabetes mellitus (GDM) or with normal pregnancies. As negative controls, tissue sections were incubated in parallel with isotype IgG at the same concentration as the primary antibody or with only secondary antibody without primary antibody. DUSP9 was observed in the cytotrophoblasts of both groups (arrows). Magnification: 200x (upper row) or 400x (lower row).

    Journal: Journal of Diabetes Research

    Article Title: Expression of Dual-Specificity Phosphatase 9 in Placenta and Its Relationship with Gestational Diabetes Mellitus

    doi: 10.1155/2019/1963178

    Figure Lengend Snippet: Immunohistochemistry showing the localization of DUSP9 protein in placental tissues from mothers with gestational diabetes mellitus (GDM) or with normal pregnancies. As negative controls, tissue sections were incubated in parallel with isotype IgG at the same concentration as the primary antibody or with only secondary antibody without primary antibody. DUSP9 was observed in the cytotrophoblasts of both groups (arrows). Magnification: 200x (upper row) or 400x (lower row).

    Article Snippet: Sections were subjected to an antigen retrieval method using citrate buffer at 98°C for 15 min. After the endogenous peroxidase was inactivated by soaking in 3% hydrogen peroxide at 37°C for 15 min, sections were incubated with the primary antibody against DUSP9 (1 : 100 dilution; Proteintech, Chicago, USA) at 37°C for 60 min, followed by the secondary antibody horseradish peroxidase-labeled polymer-conjugated goat anti-rabbit immunoglobulin G (IgG; DakoCytomation, USA) at 37°C for 45 min. Peroxidase activity was detected by incubating sections with DAB substrate, producing a brown stain.

    Techniques: Immunohistochemistry, Incubation, Concentration Assay