goat f  (Abcam)

 
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    Name:
    Goat F ab 2 Anti Rat IgG Fc TR
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    Catalog Number:
    ab6254
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    Structured Review

    Abcam goat f

    https://www.bioz.com/result/goat f/product/Abcam
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    goat f - by Bioz Stars, 2020-09
    94/100 stars

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    Incubation:

    Article Title: Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis
    Article Snippet: .. After washing with TBS/0.001% Triton, they were incubated for 1 hour at room temperature with Goat F(ab) Anti-Rabbit IgG H & L (HRP) and Goat F(ab) Anti-Mouse IgG H & L (HRP; ab7171 and ab6823 abcam, 1:500). .. After washing in TBS/0.001% Triton, the fluorescence was visualized with the tyramide kits from Perkin Elmer (NEL745B001KT and NEL744B001KT).

    Article Title: Expansion and Differentiation of Human Embryonic Stem Cells to Endoderm Progeny in a Microcarrier Stirred-Suspension Culture
    Article Snippet: .. Cells were washed with PBS, blocked with 3% normal donkey serum for 20 min, and incubated with primary antibodies: rabbit OCT3/4A, mouse SOX17, goat FOXA2, and rabbit BRACHYURY (Abcam) for 1 h at room temperature. .. Cells were washed again and incubated with appropriate Cy3- or FITC-conjugated donkey secondary antibodies for 40 min at room temperature.

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  • 99
    Abcam anti rabbit igg
    Recombinant ACKR3 and <t>AVPR1A</t> form heteromeric complexes. ( a ) Typical PLA images for the detection of individual receptors and receptor–receptor interactions in HEK293T cells transfected with DNA encoding HA- or FLAG-tagged receptors. Ctrl: cells transfected with pcDNA. Images show merged PLA/DAPI signals acquired from z -stack images ( n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm. ( b ) Quantification of PLA signals per cell as in ( a ). n = 3 independent experiments with n = 10 images per condition and experiment. ( c ) HEK293T cells expressing HA-AVPR1A and FLAG-ACKR3 were lysed (input), the lysate was immunoprecipitated (IP) with anti-HA, followed by immunoblotting (IB) to detect HA-AVPR1A (left) and FLAG-ACKR3 (right) in the IP samples. IP control: precipitate after incubation of cell lysates with <t>IgG-coupled</t> resin. ( d , e ) Intermolecular BRET assays. Cells were co-transfected with AVPR1A-hRluc plus EYFP (open circles) or ACKR3-EYFP (grey squares) at various acceptor : donor ratios ( d ) and with increasing amounts of AVPR1A-hRluc and ACKR3-EYFP at a constant ratio of 1 : 10 ( e ).
    Anti Rabbit Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg/product/Abcam
    Average 99 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg - by Bioz Stars, 2020-09
    99/100 stars
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    Recombinant ACKR3 and AVPR1A form heteromeric complexes. ( a ) Typical PLA images for the detection of individual receptors and receptor–receptor interactions in HEK293T cells transfected with DNA encoding HA- or FLAG-tagged receptors. Ctrl: cells transfected with pcDNA. Images show merged PLA/DAPI signals acquired from z -stack images ( n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm. ( b ) Quantification of PLA signals per cell as in ( a ). n = 3 independent experiments with n = 10 images per condition and experiment. ( c ) HEK293T cells expressing HA-AVPR1A and FLAG-ACKR3 were lysed (input), the lysate was immunoprecipitated (IP) with anti-HA, followed by immunoblotting (IB) to detect HA-AVPR1A (left) and FLAG-ACKR3 (right) in the IP samples. IP control: precipitate after incubation of cell lysates with IgG-coupled resin. ( d , e ) Intermolecular BRET assays. Cells were co-transfected with AVPR1A-hRluc plus EYFP (open circles) or ACKR3-EYFP (grey squares) at various acceptor : donor ratios ( d ) and with increasing amounts of AVPR1A-hRluc and ACKR3-EYFP at a constant ratio of 1 : 10 ( e ).

    Journal: Open Biology

    Article Title: Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle

    doi: 10.1098/rsob.170207

    Figure Lengend Snippet: Recombinant ACKR3 and AVPR1A form heteromeric complexes. ( a ) Typical PLA images for the detection of individual receptors and receptor–receptor interactions in HEK293T cells transfected with DNA encoding HA- or FLAG-tagged receptors. Ctrl: cells transfected with pcDNA. Images show merged PLA/DAPI signals acquired from z -stack images ( n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm. ( b ) Quantification of PLA signals per cell as in ( a ). n = 3 independent experiments with n = 10 images per condition and experiment. ( c ) HEK293T cells expressing HA-AVPR1A and FLAG-ACKR3 were lysed (input), the lysate was immunoprecipitated (IP) with anti-HA, followed by immunoblotting (IB) to detect HA-AVPR1A (left) and FLAG-ACKR3 (right) in the IP samples. IP control: precipitate after incubation of cell lysates with IgG-coupled resin. ( d , e ) Intermolecular BRET assays. Cells were co-transfected with AVPR1A-hRluc plus EYFP (open circles) or ACKR3-EYFP (grey squares) at various acceptor : donor ratios ( d ) and with increasing amounts of AVPR1A-hRluc and ACKR3-EYFP at a constant ratio of 1 : 10 ( e ).

    Article Snippet: A total of 50 µg of rabbit anti-AVPR1A (Bioss BS-11598R), mouse anti-HA (Bioss bsm-50131M) or anti- rabbit IgG (AbCam Ab27478) were incubated with 50 µl of Amino Link Plus coupling resin for 180 min at room temperature.

    Techniques: Recombinant, Proximity Ligation Assay, Transfection, Expressing, Immunoprecipitation, Incubation, Bioluminescence Resonance Energy Transfer

    ACKR3 and AVPR1A form heteromeric complexes in hVSMCs. ( a ) Representative PLA images for the detection of individual receptors, receptor–receptor complexes and pMLC2 in hVSMC. PLA for pMLC2 was performed in non-permeabilized cells (pMLC2, bottom left) and permeabilized cells (pMLC2, permeabilized cells, bottom right). All other PLAs were performed in non-permeabilized cells. Ctrl: omission of one primary antibody. Images show merged PLA/DAPI signals acquired from z -stack images ( n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm. ( b ) Quantification of PLA signals per cell as in ( a ). n = 4 independent experiments with n = 10 images per condition and experiment. ( c ) Three-dimensional representations of ACKR3 : AVPR1A interactions in hVSMC. Deconvolved images were generated from z -stack images ( n = 20; thickness: 0.5 µm, bottom to top). Images show merged PLA/DAPI signals. ( d–g ) hVSMCs were lysed (=input) and AVPR1A was immunoprecipitated (IP) followed by immunoblotting (IB) to detect AVPR1A ( d ), CXCR4 ( e ), ACKR3 ( f ) and β 2 -AR ( g ) in the IP samples. IP control: precipitate after incubation of cell lysates with IgG-coupled resin. Images are representative of n = 4 independent experiments.

    Journal: Open Biology

    Article Title: Identification and functional characterization of arginine vasopressin receptor 1A : atypical chemokine receptor 3 heteromers in vascular smooth muscle

    doi: 10.1098/rsob.170207

    Figure Lengend Snippet: ACKR3 and AVPR1A form heteromeric complexes in hVSMCs. ( a ) Representative PLA images for the detection of individual receptors, receptor–receptor complexes and pMLC2 in hVSMC. PLA for pMLC2 was performed in non-permeabilized cells (pMLC2, bottom left) and permeabilized cells (pMLC2, permeabilized cells, bottom right). All other PLAs were performed in non-permeabilized cells. Ctrl: omission of one primary antibody. Images show merged PLA/DAPI signals acquired from z -stack images ( n = 10; thickness 1 µm, bottom to top). Scale bars, 10 µm. ( b ) Quantification of PLA signals per cell as in ( a ). n = 4 independent experiments with n = 10 images per condition and experiment. ( c ) Three-dimensional representations of ACKR3 : AVPR1A interactions in hVSMC. Deconvolved images were generated from z -stack images ( n = 20; thickness: 0.5 µm, bottom to top). Images show merged PLA/DAPI signals. ( d–g ) hVSMCs were lysed (=input) and AVPR1A was immunoprecipitated (IP) followed by immunoblotting (IB) to detect AVPR1A ( d ), CXCR4 ( e ), ACKR3 ( f ) and β 2 -AR ( g ) in the IP samples. IP control: precipitate after incubation of cell lysates with IgG-coupled resin. Images are representative of n = 4 independent experiments.

    Article Snippet: A total of 50 µg of rabbit anti-AVPR1A (Bioss BS-11598R), mouse anti-HA (Bioss bsm-50131M) or anti- rabbit IgG (AbCam Ab27478) were incubated with 50 µl of Amino Link Plus coupling resin for 180 min at room temperature.

    Techniques: Proximity Ligation Assay, Generated, Immunoprecipitation, Incubation

    FKN and CX3CR1 mediate WEHI 274.1 recruitment to injured atherosclerotic carotid arteries. ( A ) WEHI 274.1 adhesion to mechanically injured atherosclerotic carotids was studied over 30 minutes in the presence of an IgG control antibody (black bars) or a function blocking anti-FKN antibody (white bars). ( B ) Representative intravital microscopic images from mouse carotid arteries at baseline (left) and 15 minutes (right) after injury of the atherosclerotic vascular wall, pretreated with either an anti-FKN antibody (upper row) or an isotype IgG control antibody (lower row). WEHI 274.1 cells were stained with DCF (green). Bars, 50 µm. n = 4–8, *p

    Journal: PLoS ONE

    Article Title: Fractalkine Is Expressed in Early and Advanced Atherosclerotic Lesions and Supports Monocyte Recruitment via CX3CR1

    doi: 10.1371/journal.pone.0043572

    Figure Lengend Snippet: FKN and CX3CR1 mediate WEHI 274.1 recruitment to injured atherosclerotic carotid arteries. ( A ) WEHI 274.1 adhesion to mechanically injured atherosclerotic carotids was studied over 30 minutes in the presence of an IgG control antibody (black bars) or a function blocking anti-FKN antibody (white bars). ( B ) Representative intravital microscopic images from mouse carotid arteries at baseline (left) and 15 minutes (right) after injury of the atherosclerotic vascular wall, pretreated with either an anti-FKN antibody (upper row) or an isotype IgG control antibody (lower row). WEHI 274.1 cells were stained with DCF (green). Bars, 50 µm. n = 4–8, *p

    Article Snippet: Rat IgG2b and rabbit IgG, used as negative controls, were from Abcam and Dako, respectively ( ).

    Techniques: Blocking Assay, Staining

    Determination of the DAO-binding domain within BSN. Shown is a schematic BSN diagram depicting the functional domains: double zinc finger domains ( Zn1 and Zn2 ) and predicted coiled-coil regions ( cc1 , cc2 , and cc3 ). A stable HEK293 cell line expressing DAO was transfected with either BSN 95–3938 ( A ), BSN 95–609 ( B ), BSN 95–3263 ( C ), BSN 1692–3263 ( D ), or BSN 2715–3263 ( E ), each fused to GFP at its N terminus. Lysates were subjected to immunoprecipitation ( IP ) with a mouse anti-GFP antibody ( lane 3 ) or nonspecific mouse IgG ( lane 2 ) and immunoblotted with an anti-GFP antibody ( top ) or an anti-DAO antibody ( bottom ). The combined data suggest that the domain required for the interaction with DAO resides between amino acids 2715 and 3263 of the BSN polypeptide.

    Journal: The Journal of Biological Chemistry

    Article Title: d

    doi: 10.1074/jbc.M111.262063

    Figure Lengend Snippet: Determination of the DAO-binding domain within BSN. Shown is a schematic BSN diagram depicting the functional domains: double zinc finger domains ( Zn1 and Zn2 ) and predicted coiled-coil regions ( cc1 , cc2 , and cc3 ). A stable HEK293 cell line expressing DAO was transfected with either BSN 95–3938 ( A ), BSN 95–609 ( B ), BSN 95–3263 ( C ), BSN 1692–3263 ( D ), or BSN 2715–3263 ( E ), each fused to GFP at its N terminus. Lysates were subjected to immunoprecipitation ( IP ) with a mouse anti-GFP antibody ( lane 3 ) or nonspecific mouse IgG ( lane 2 ) and immunoblotted with an anti-GFP antibody ( top ) or an anti-DAO antibody ( bottom ). The combined data suggest that the domain required for the interaction with DAO resides between amino acids 2715 and 3263 of the BSN polypeptide.

    Article Snippet: The primary antibodies used in this study for Western blotting include catalase (Sigma), BSN (StressGen), PSD-95 (NeuroMab), MAP2 (Sigma), GFP (Clontech), mouse IgG (Santa Cruz Biotechnology, Inc.), and rabbit IgG (Abcam).

    Techniques: Binding Assay, Functional Assay, Expressing, Transfection, Immunoprecipitation

    Immunohistochemical co-staining for TLR2 and TLR9 with NeuN and GFAP . IHC co-staining of TLR2 and TLR9 with specific markers for neurons (NeuN) and astrocytes (GFAP) was performed and observed under a confocal microscope. The first antibodies used were rabbit anti-TLR2, rabbit anti-TLR9, rat anti-GFAP and mouse Fluor 488 conjugated anti-NeuN. The secondary antibodies used were Texas red conjugated donkey antibody to rabbit IgG and Cy2 conjugated goat antibody to rat IgG. The images show that TLR2 staining co-localized with neurons in the cortex of injured brain ( A, B, C ). However, co-localization of TLR2 with astrocytes was hardly observed in the brain section ( D, E, F ). Weak expression of TLR9 was observed co-localized with neurons in cortex of the injured brain ( G, H, I ). Co-localization of TLR9 with astrocytes was detected in the white matter of periventricular and subcortical regions of the brain ( J, K, L ).

    Journal: Journal of Neuroinflammation

    Article Title: Genomic profile of Toll-like receptor pathways in traumatically brain-injured mice: effect of exogenous progesterone

    doi: 10.1186/1742-2094-8-42

    Figure Lengend Snippet: Immunohistochemical co-staining for TLR2 and TLR9 with NeuN and GFAP . IHC co-staining of TLR2 and TLR9 with specific markers for neurons (NeuN) and astrocytes (GFAP) was performed and observed under a confocal microscope. The first antibodies used were rabbit anti-TLR2, rabbit anti-TLR9, rat anti-GFAP and mouse Fluor 488 conjugated anti-NeuN. The secondary antibodies used were Texas red conjugated donkey antibody to rabbit IgG and Cy2 conjugated goat antibody to rat IgG. The images show that TLR2 staining co-localized with neurons in the cortex of injured brain ( A, B, C ). However, co-localization of TLR2 with astrocytes was hardly observed in the brain section ( D, E, F ). Weak expression of TLR9 was observed co-localized with neurons in cortex of the injured brain ( G, H, I ). Co-localization of TLR9 with astrocytes was detected in the white matter of periventricular and subcortical regions of the brain ( J, K, L ).

    Article Snippet: The secondary antibodies used were Texas red conjugated donkey antibody to rabbit IgG (Abcam) and Cy2 conjugated goat antibody to rat IgG (Gene Tex, Inc, Irvine, CA).

    Techniques: Immunohistochemistry, Staining, Microscopy, Expressing