goat f ab anti mouse igg h l  (Abcam)

 
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    Name:
    Goat F ab Anti Mouse IgG H L
    Description:

    Catalog Number:
    ab6668
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    Structured Review

    Abcam goat f ab anti mouse igg h l

    https://www.bioz.com/result/goat f ab anti mouse igg h l/product/Abcam
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    goat f ab anti mouse igg h l - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: The H2B deubiquitinase Usp22 promotes antibody class switch recombination by facilitating non-homologous end joining
    Article Snippet: .. The slides were blocked with 10% BSA (BioShop) plus goat F(ab) polyclonal antibody against mouse IgG (Abcam, catalog number: ab6668; 1/100 dilution), to decrease nonspecific staining. .. The γH2AX staining using mouse monoclonal antibody against γH2AX (Millipore, clone: JBW301, catalog number: 05–636; 1/250 dilution) and quantification of foci formation were performed as we previously described .

    Incubation:

    Article Title: Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma
    Article Snippet: .. Slides were then incubated in 1% H2 O2 for 15 min to quench endogenous peroxidase activity, followed by serum blocking for 1 h. Goat anti-mouse IgG Fab (Abcam, ab6668) was added for 1 h to block endogenous IgG. .. After washing in PBST, slides were incubated with primary against ki67 (Abcam, ab8191) or primary against MRP1 (Santa Cruz, sc-18835) overnight in a moisture chamber at 4 °C.

    Activity Assay:

    Article Title: Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma
    Article Snippet: .. Slides were then incubated in 1% H2 O2 for 15 min to quench endogenous peroxidase activity, followed by serum blocking for 1 h. Goat anti-mouse IgG Fab (Abcam, ab6668) was added for 1 h to block endogenous IgG. .. After washing in PBST, slides were incubated with primary against ki67 (Abcam, ab8191) or primary against MRP1 (Santa Cruz, sc-18835) overnight in a moisture chamber at 4 °C.

    Blocking Assay:

    Article Title: IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95
    Article Snippet: .. The following antibodies were used for blocking: goat F(ab) anti-mouse IgG H & L: ab6668 (Abcam) and anti-CD16/CD32 monoclonal antibody (93): 14-0161 (Thermo Fisher Scientific). .. The following antibodies and dye were used for staining: anti-IgSF11: sc-393816 (Santa Cruz Biotechnology), Alexa Fluor 488 goat anti-mouse IgG (H + L): A11029 (Thermo Fisher Scientific), and TO-PRO-3 iodide: T3605 (Thermo Fisher Scientific).

    Article Title: Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma
    Article Snippet: .. Slides were then incubated in 1% H2 O2 for 15 min to quench endogenous peroxidase activity, followed by serum blocking for 1 h. Goat anti-mouse IgG Fab (Abcam, ab6668) was added for 1 h to block endogenous IgG. .. After washing in PBST, slides were incubated with primary against ki67 (Abcam, ab8191) or primary against MRP1 (Santa Cruz, sc-18835) overnight in a moisture chamber at 4 °C.

    Article Title: Determination of a Critical Size Threshold for Volumetric Muscle Loss in the Mouse Quadriceps
    Article Snippet: .. When antimouse secondary antibodies were used, an additional wash with Goat F(ab) antimouse IgG (Abcam; ab6668, 2 μg/mL in blocking buffer) was performed for 1 h. Samples were washed between steps with 0.1% Triton in PBS. .. Primary antibodies were diluted in blocking buffer at 1:200 for dystrophin (Abcam; ab15277), von Willebrand factor (vWF) (Abcam; ab6994), and CD68 (Abcam; ab125212).

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  • 99
    Abcam rabbit anti fsp 1
    Pentoxifylline treatment reduced the expression of fibrosis marker FSP1. (A–B) Immunohistochemistry for <t>FSP-1</t> was performed on mice peritoneal tissue in the different groups at day 3 and day 7. The FSP-1 expression was increased in the PA group. Representative images of the sham group, the PA group, and the PA+PTX group are shown. (Original magnification, x100, bar = 100 µm). (C) Quantification of FSP-1 + cells (%) in high-powered field (HPF) at x400 magnification. Data are expressed as the mean±SE. ∗ P
    Rabbit Anti Fsp 1, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti fsp 1/product/Abcam
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    rabbit anti fsp 1 - by Bioz Stars, 2020-09
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    95
    Abcam alexa fluor 594
    Representative immunofluorescence images of IOSE and OVCA433 cells stained with primary anti-E-cadherin and anti-N-cadherin antibodies and a secondary antibody conjugated with <t>Alexa</t> Fluor 488 and Alexa Fluor 594, respectively. ( A ) Normal and ( B ) high-grade models. Scale bar = 50 µm.
    Alexa Fluor 594, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 594/product/Abcam
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 - by Bioz Stars, 2020-09
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    igg  (Abcam)
    99
    Abcam igg
    NK cell depletion reduces T FH cell responses at later times during chronic virus infection. WT B6 mice were treated with PK136 (Δ NK) or control antibody at days −2 and −3 before infection with LCMV-Clone13. Splenocytes were harvested at day 29 pi for analysis. (A) An example of CD4 and I-Ab/GP 66–77 tetramer staining on all splenocytes. (B) The I-Ab/GP 66–77 tetramer+ CD4 cell frequency among all splenocytes (left) and the total number of I-Ab/GP 66–77 tetramer+ CD4 T cells (right) within the spleen at day 29. (C) An example of CD4 and CXCR5 staining on gated CD4 + I-Ab/GP 66–77 + CD8 − CD19 − cells. (D) The CXCR5 + cell frequency among tetramer+ CD4 cells (left) and the total numbers of I-Ab/GP 66–77 tetramer+ T FH cells within the spleen at day 29. (E) An example of CXCR5 and ICOS staining on bulk-gated CD4 + CD8 − CD19 − cells. (F) The CXCR5 + ICOS + cell frequency among CD4 + T cells (left) and the total numbers of CXCR5 + ICOS + T FH cells within the spleen at day 29 (right). (G) The serum levels of anti-LCMV total <t>IgG,</t> <t>IgG1,</t> and IgG2c were measured by ELISA. (H) The total number of GC B cells (left) and plasmablast B cells (right)/spleen. (I) The level of infectious virus was measured by plaque assay in the serum, liver, lung, and kidney. The dashed line indicates the limit of detection. (J) The serum levels of anti-LCMV total IgG across time in Armstrong- or Clone13-infected mice were measured by ELISA. (A–I) Data represent 6–9 mice from 3 experiments. (J) Data are from 1 experiment, representative of 3, with 3 mice/group. * P
    Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg/product/Abcam
    Average 99 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    igg - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Pentoxifylline treatment reduced the expression of fibrosis marker FSP1. (A–B) Immunohistochemistry for FSP-1 was performed on mice peritoneal tissue in the different groups at day 3 and day 7. The FSP-1 expression was increased in the PA group. Representative images of the sham group, the PA group, and the PA+PTX group are shown. (Original magnification, x100, bar = 100 µm). (C) Quantification of FSP-1 + cells (%) in high-powered field (HPF) at x400 magnification. Data are expressed as the mean±SE. ∗ P

    Journal: PeerJ

    Article Title: Pentoxifylline decreases post-operative intra-abdominal adhesion formation in an animal model

    doi: 10.7717/peerj.5434

    Figure Lengend Snippet: Pentoxifylline treatment reduced the expression of fibrosis marker FSP1. (A–B) Immunohistochemistry for FSP-1 was performed on mice peritoneal tissue in the different groups at day 3 and day 7. The FSP-1 expression was increased in the PA group. Representative images of the sham group, the PA group, and the PA+PTX group are shown. (Original magnification, x100, bar = 100 µm). (C) Quantification of FSP-1 + cells (%) in high-powered field (HPF) at x400 magnification. Data are expressed as the mean±SE. ∗ P

    Article Snippet: Sections were incubated with primary antibodies, rabbit anti-ki67 (1:200, Abcam, Cambridge, MA, USA), rat anti-F4/80 (1:200, Abcam) or rabbit anti-FSP-1 (1:200, Abcam) overnight at 4 °C.

    Techniques: Expressing, Marker, Immunohistochemistry, Mouse Assay

    Isolation of rat antigen-specific plasma/plasmablast cells (ASPCs) using fluorescence-activated cell sorting (FACS) . (A) A representative FACS graph of the lymphocytes from green fluorescent protein (GFP)-immunized rats stained with anti-rat IgG Dylight 650, ER-tracker and GFP-Dylight 488. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. Plasma/plasmablast cell (PCs) were gated as IgG low endoplasmic reticulum (ER) high (R2). The R2-gated cells were further subdivided into the ASPCs (IgG low ER high GFP + , R3 gate) and non-specific PCs (IgG low ER high GFP - , R4 gate). The numbers indicate the mean percentages of cells in the gated area from three separate experiments. (B) The R3-gated and R4-gated cells stained intracellularly with anti-rat IgG Dylight 594 (red) and GFP Dylight 488 (green). The numbers indicate the mean percentages of cells with IgG and GFP signals from three separate experiments. (C) Representative agarose gel electrophoresis of cognate pairs of V genes amplified from single-cell-sorted R3-gated and R4-gated cells. (D) Antigen specificity of the rat monoclonal antibodies (mAbs) produced from R3-gated and R4-gated cells. Cognate pairs of linear IgH and IgL genes were cotransfected into 293FT cells. The antigen specificity of the mAbs was expressed as relative light units (RLU)/IgG.

    Journal: BMC Biology

    Article Title: Rapid production of antigen-specific monoclonal antibodies from a variety of animals

    doi: 10.1186/1741-7007-10-80

    Figure Lengend Snippet: Isolation of rat antigen-specific plasma/plasmablast cells (ASPCs) using fluorescence-activated cell sorting (FACS) . (A) A representative FACS graph of the lymphocytes from green fluorescent protein (GFP)-immunized rats stained with anti-rat IgG Dylight 650, ER-tracker and GFP-Dylight 488. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. Plasma/plasmablast cell (PCs) were gated as IgG low endoplasmic reticulum (ER) high (R2). The R2-gated cells were further subdivided into the ASPCs (IgG low ER high GFP + , R3 gate) and non-specific PCs (IgG low ER high GFP - , R4 gate). The numbers indicate the mean percentages of cells in the gated area from three separate experiments. (B) The R3-gated and R4-gated cells stained intracellularly with anti-rat IgG Dylight 594 (red) and GFP Dylight 488 (green). The numbers indicate the mean percentages of cells with IgG and GFP signals from three separate experiments. (C) Representative agarose gel electrophoresis of cognate pairs of V genes amplified from single-cell-sorted R3-gated and R4-gated cells. (D) Antigen specificity of the rat monoclonal antibodies (mAbs) produced from R3-gated and R4-gated cells. Cognate pairs of linear IgH and IgL genes were cotransfected into 293FT cells. The antigen specificity of the mAbs was expressed as relative light units (RLU)/IgG.

    Article Snippet: Materials Antibodies against rat IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and alkaline phosphatase (AP) conjugated), guinea pig IgG (unconjugated, Dylight 488 conjugated and AP conjugated), rabbit IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and AP conjugated) and human IgG (Dylight 488 conjugated) were obtained from Abcam.

    Techniques: Isolation, Fluorescence, FACS, Staining, Agarose Gel Electrophoresis, Amplification, Produced

    Identification of mouse plasma/plasmablast cells (PCs) with a fluorescent dye specific for the endoplasmic reticulum (ER) . (A) Cytology images of mouse lymph node cells stained with anti-mouse IgG (green) and ER-tracker (blue). The arrow indicates the IgG low ER high cells. (B) A representative fluorescence-activated cell sorting (FACS) graph of cells prepared from the lymph nodes of egg albumin-immunized mice. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. The R1-gated cells were stained with anti-mouse IgG Dylight 488 and ER-tracker, and the IgG low ER high cells (R2) were further analyzed for CD38-APC versus CD45R-PE surface expression using FACS. The relative number of cells in each region is given as a mean percentage of three separate experiments. (C) FACS-sorted R1-gated, R2-gated and R3-gated cells stained intracellularly with anti-mouse IgG Dylight 488 (green) and 4',6-diamidino-2-phenylindole (DAPI; blue). The numbers indicate the mean percentages of the cells with intense cytoplasmic IgG from three separate experiments.

    Journal: BMC Biology

    Article Title: Rapid production of antigen-specific monoclonal antibodies from a variety of animals

    doi: 10.1186/1741-7007-10-80

    Figure Lengend Snippet: Identification of mouse plasma/plasmablast cells (PCs) with a fluorescent dye specific for the endoplasmic reticulum (ER) . (A) Cytology images of mouse lymph node cells stained with anti-mouse IgG (green) and ER-tracker (blue). The arrow indicates the IgG low ER high cells. (B) A representative fluorescence-activated cell sorting (FACS) graph of cells prepared from the lymph nodes of egg albumin-immunized mice. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. The R1-gated cells were stained with anti-mouse IgG Dylight 488 and ER-tracker, and the IgG low ER high cells (R2) were further analyzed for CD38-APC versus CD45R-PE surface expression using FACS. The relative number of cells in each region is given as a mean percentage of three separate experiments. (C) FACS-sorted R1-gated, R2-gated and R3-gated cells stained intracellularly with anti-mouse IgG Dylight 488 (green) and 4',6-diamidino-2-phenylindole (DAPI; blue). The numbers indicate the mean percentages of the cells with intense cytoplasmic IgG from three separate experiments.

    Article Snippet: Materials Antibodies against rat IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and alkaline phosphatase (AP) conjugated), guinea pig IgG (unconjugated, Dylight 488 conjugated and AP conjugated), rabbit IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and AP conjugated) and human IgG (Dylight 488 conjugated) were obtained from Abcam.

    Techniques: Staining, Fluorescence, FACS, Mouse Assay, Expressing

    Human plasma/plasmablast cell (PC) isolation by fluorescence-activated cell sorting (FACS) using ER-tracker and anti-human IgG . (A) A representative FACS graph of cells prepared from the lymph nodes of cancer patients. Cells were stained with anti-human IgG Dylight 488 and ER-tracker, and the IgG low endoplasmic reticulum (ER) high cells were further analyzed for CD38-APC versus CD20-PE surface expression using FACS. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. PCs are defined herein as CD38 high CD20 low cells. A representative FACS graph of three separate experiments is shown. The relative number of cells in each region is given as a mean percentage of three separate experiments. (B) R1-gated and R2-gated cells stained intracellularly with anti-human IgG Dylight 488 (green). Nuclei are stained with 4',6-diamidino-2-phenylindole (DAPI; blue). The numbers indicate the percentages of cells with intense cytoplasmic IgG. (C) Representative agarose gel electrophoresis of cognate pairs of V genes amplified from single-cell-sorted R3-gated cells. (D) Polymerase chain reaction (PCR) success ratio for V genes from single-cell-sorted R3-gated cells from lymph nodes of patients with colon and gallbladder cancers.

    Journal: BMC Biology

    Article Title: Rapid production of antigen-specific monoclonal antibodies from a variety of animals

    doi: 10.1186/1741-7007-10-80

    Figure Lengend Snippet: Human plasma/plasmablast cell (PC) isolation by fluorescence-activated cell sorting (FACS) using ER-tracker and anti-human IgG . (A) A representative FACS graph of cells prepared from the lymph nodes of cancer patients. Cells were stained with anti-human IgG Dylight 488 and ER-tracker, and the IgG low endoplasmic reticulum (ER) high cells were further analyzed for CD38-APC versus CD20-PE surface expression using FACS. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. PCs are defined herein as CD38 high CD20 low cells. A representative FACS graph of three separate experiments is shown. The relative number of cells in each region is given as a mean percentage of three separate experiments. (B) R1-gated and R2-gated cells stained intracellularly with anti-human IgG Dylight 488 (green). Nuclei are stained with 4',6-diamidino-2-phenylindole (DAPI; blue). The numbers indicate the percentages of cells with intense cytoplasmic IgG. (C) Representative agarose gel electrophoresis of cognate pairs of V genes amplified from single-cell-sorted R3-gated cells. (D) Polymerase chain reaction (PCR) success ratio for V genes from single-cell-sorted R3-gated cells from lymph nodes of patients with colon and gallbladder cancers.

    Article Snippet: Materials Antibodies against rat IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and alkaline phosphatase (AP) conjugated), guinea pig IgG (unconjugated, Dylight 488 conjugated and AP conjugated), rabbit IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and AP conjugated) and human IgG (Dylight 488 conjugated) were obtained from Abcam.

    Techniques: Isolation, Fluorescence, FACS, Staining, Expressing, Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

    Isolation of rabbit antigen-specific plasma/plasmablast cells (ASPCs) using fluorescence-activated cell sorting (FACS) . (A) A representative FACS graph of the lymphocytes of green fluorescent protein (GFP)-immunized rabbits stained with anti-rabbit IgG Dylight 650, ER-tracker and GFP Dylight 488. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. Plasma/plasmablast cells (PCs) were gated as IgG low endoplasmic reticulum (ER) high (R2). The R2-gated cells were further subdivided into the ASPCs (IgG low ER high GFP + , R3 gate) and non-specific PCs (IgG low ER high GFP - , R4 gate). The numbers indicate the mean percentages of cells in the gated area from three separate experiments. (B) R1-gated, R2-gated, R3-gated and R4-gated cells stained intracellularly with anti-rabbit IgG Dylight 594 (red) and GFP-Dylight 488 (green). The numbers indicate the mean percentages of cells exhibiting IgG and GFP signals from two separate experiments. (C) A representative agarose gel electrophoresis of cognate pairs of V genes amplified from single-cell-sorted R3-gated and R4-gated cells (upper). Polymerase chain reaction (PCR) success ratio for V genes from single-cell-sorted R3-gated and R4-gated cells (lower). (D) The antigen specificity of rabbit monoclonal antibodies (mAbs) produced from R3-gated and R4-gated cells. Cognate pairs of linear IgH and IgL genes were cotransfected into 293FT cells, and the concentration of antibodies in the cell culture supernatant was determined (upper). The antigen specificity of the mAbs was expressed as relative light units (RLU)/IgG (lower).

    Journal: BMC Biology

    Article Title: Rapid production of antigen-specific monoclonal antibodies from a variety of animals

    doi: 10.1186/1741-7007-10-80

    Figure Lengend Snippet: Isolation of rabbit antigen-specific plasma/plasmablast cells (ASPCs) using fluorescence-activated cell sorting (FACS) . (A) A representative FACS graph of the lymphocytes of green fluorescent protein (GFP)-immunized rabbits stained with anti-rabbit IgG Dylight 650, ER-tracker and GFP Dylight 488. The forward-versus-side-scatter (FSC vs SSC) with gate R1 represents lymphocytes. Plasma/plasmablast cells (PCs) were gated as IgG low endoplasmic reticulum (ER) high (R2). The R2-gated cells were further subdivided into the ASPCs (IgG low ER high GFP + , R3 gate) and non-specific PCs (IgG low ER high GFP - , R4 gate). The numbers indicate the mean percentages of cells in the gated area from three separate experiments. (B) R1-gated, R2-gated, R3-gated and R4-gated cells stained intracellularly with anti-rabbit IgG Dylight 594 (red) and GFP-Dylight 488 (green). The numbers indicate the mean percentages of cells exhibiting IgG and GFP signals from two separate experiments. (C) A representative agarose gel electrophoresis of cognate pairs of V genes amplified from single-cell-sorted R3-gated and R4-gated cells (upper). Polymerase chain reaction (PCR) success ratio for V genes from single-cell-sorted R3-gated and R4-gated cells (lower). (D) The antigen specificity of rabbit monoclonal antibodies (mAbs) produced from R3-gated and R4-gated cells. Cognate pairs of linear IgH and IgL genes were cotransfected into 293FT cells, and the concentration of antibodies in the cell culture supernatant was determined (upper). The antigen specificity of the mAbs was expressed as relative light units (RLU)/IgG (lower).

    Article Snippet: Materials Antibodies against rat IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and alkaline phosphatase (AP) conjugated), guinea pig IgG (unconjugated, Dylight 488 conjugated and AP conjugated), rabbit IgG (unconjugated, Dylight 594 conjugated, Dylight 650 conjugated and AP conjugated) and human IgG (Dylight 488 conjugated) were obtained from Abcam.

    Techniques: Isolation, Fluorescence, FACS, Staining, Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction, Produced, Concentration Assay, Cell Culture

    Representative immunofluorescence images of IOSE and OVCA433 cells stained with primary anti-E-cadherin and anti-N-cadherin antibodies and a secondary antibody conjugated with Alexa Fluor 488 and Alexa Fluor 594, respectively. ( A ) Normal and ( B ) high-grade models. Scale bar = 50 µm.

    Journal: Cancers

    Article Title: Role of Collagen Fiber Morphology on Ovarian Cancer Cell Migration Using Image-Based Models of the Extracellular Matrix

    doi: 10.3390/cancers12061390

    Figure Lengend Snippet: Representative immunofluorescence images of IOSE and OVCA433 cells stained with primary anti-E-cadherin and anti-N-cadherin antibodies and a secondary antibody conjugated with Alexa Fluor 488 and Alexa Fluor 594, respectively. ( A ) Normal and ( B ) high-grade models. Scale bar = 50 µm.

    Article Snippet: For cadherin staining, the cells were incubated with an anti-E-cadherin (mouse, ab1416, Abcam) and anti-N-cadherin (rabbit, ab18203, Abcam, Cambridge, UK) primary antibody (at 1:200 dilution) overnight at 4 °C, followed by incubation with Alexa Fluor 488 (goat anti-rabbit IgG (H & L), ab150077, Abcam) and Alexa Fluor 594 (goat anti-mouse IgG (H & L), ab150116, Abcam) secondary antibody, respectively, for 1 h at room temperature.

    Techniques: Immunofluorescence, Staining

    NK cell depletion reduces T FH cell responses at later times during chronic virus infection. WT B6 mice were treated with PK136 (Δ NK) or control antibody at days −2 and −3 before infection with LCMV-Clone13. Splenocytes were harvested at day 29 pi for analysis. (A) An example of CD4 and I-Ab/GP 66–77 tetramer staining on all splenocytes. (B) The I-Ab/GP 66–77 tetramer+ CD4 cell frequency among all splenocytes (left) and the total number of I-Ab/GP 66–77 tetramer+ CD4 T cells (right) within the spleen at day 29. (C) An example of CD4 and CXCR5 staining on gated CD4 + I-Ab/GP 66–77 + CD8 − CD19 − cells. (D) The CXCR5 + cell frequency among tetramer+ CD4 cells (left) and the total numbers of I-Ab/GP 66–77 tetramer+ T FH cells within the spleen at day 29. (E) An example of CXCR5 and ICOS staining on bulk-gated CD4 + CD8 − CD19 − cells. (F) The CXCR5 + ICOS + cell frequency among CD4 + T cells (left) and the total numbers of CXCR5 + ICOS + T FH cells within the spleen at day 29 (right). (G) The serum levels of anti-LCMV total IgG, IgG1, and IgG2c were measured by ELISA. (H) The total number of GC B cells (left) and plasmablast B cells (right)/spleen. (I) The level of infectious virus was measured by plaque assay in the serum, liver, lung, and kidney. The dashed line indicates the limit of detection. (J) The serum levels of anti-LCMV total IgG across time in Armstrong- or Clone13-infected mice were measured by ELISA. (A–I) Data represent 6–9 mice from 3 experiments. (J) Data are from 1 experiment, representative of 3, with 3 mice/group. * P

    Journal: Journal of Leukocyte Biology

    Article Title: NK cells inhibit humoral immunity by reducing the abundance of CD4+ T follicular helper cells during a chronic virus infection

    doi: 10.1189/jlb.4HI1214-594R

    Figure Lengend Snippet: NK cell depletion reduces T FH cell responses at later times during chronic virus infection. WT B6 mice were treated with PK136 (Δ NK) or control antibody at days −2 and −3 before infection with LCMV-Clone13. Splenocytes were harvested at day 29 pi for analysis. (A) An example of CD4 and I-Ab/GP 66–77 tetramer staining on all splenocytes. (B) The I-Ab/GP 66–77 tetramer+ CD4 cell frequency among all splenocytes (left) and the total number of I-Ab/GP 66–77 tetramer+ CD4 T cells (right) within the spleen at day 29. (C) An example of CD4 and CXCR5 staining on gated CD4 + I-Ab/GP 66–77 + CD8 − CD19 − cells. (D) The CXCR5 + cell frequency among tetramer+ CD4 cells (left) and the total numbers of I-Ab/GP 66–77 tetramer+ T FH cells within the spleen at day 29. (E) An example of CXCR5 and ICOS staining on bulk-gated CD4 + CD8 − CD19 − cells. (F) The CXCR5 + ICOS + cell frequency among CD4 + T cells (left) and the total numbers of CXCR5 + ICOS + T FH cells within the spleen at day 29 (right). (G) The serum levels of anti-LCMV total IgG, IgG1, and IgG2c were measured by ELISA. (H) The total number of GC B cells (left) and plasmablast B cells (right)/spleen. (I) The level of infectious virus was measured by plaque assay in the serum, liver, lung, and kidney. The dashed line indicates the limit of detection. (J) The serum levels of anti-LCMV total IgG across time in Armstrong- or Clone13-infected mice were measured by ELISA. (A–I) Data represent 6–9 mice from 3 experiments. (J) Data are from 1 experiment, representative of 3, with 3 mice/group. * P

    Article Snippet: The following antibodies were used for detection of antiviral antibodies: total IgM and IgG were detected with HRP-conjugated goat polyclonal antibody to mouse IgM and IgG, respectively (Abcam, Cambridge, MA, USA); IgG1 was detected with biotin-XX-goat anti-mouse IgG1 (Invitrogen, Carlsbad, CA, USA) and HRP-avidin D (Vector Laboratories, Burlingame, CA, USA); and IgG2c was detected with HRP-conjugated AffiniPure goat anti-mouse IgG Fcγ subclass 2c (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

    Techniques: Infection, Mouse Assay, Staining, Enzyme-linked Immunosorbent Assay, Plaque Assay

    Persistent infection enhances serum levels of antiviral antibody over time. WT B6 mice were infected with LCMV-Armstrong or -Clone13. The serum levels of anti-LCMV IgM (A), total IgG (B), IgG1 (C), and IgG2c (D) at days 8, 29, and 60 pi were measured by ELISA. The data represent 6 mice from 2 experiments. * P

    Journal: Journal of Leukocyte Biology

    Article Title: NK cells inhibit humoral immunity by reducing the abundance of CD4+ T follicular helper cells during a chronic virus infection

    doi: 10.1189/jlb.4HI1214-594R

    Figure Lengend Snippet: Persistent infection enhances serum levels of antiviral antibody over time. WT B6 mice were infected with LCMV-Armstrong or -Clone13. The serum levels of anti-LCMV IgM (A), total IgG (B), IgG1 (C), and IgG2c (D) at days 8, 29, and 60 pi were measured by ELISA. The data represent 6 mice from 2 experiments. * P

    Article Snippet: The following antibodies were used for detection of antiviral antibodies: total IgM and IgG were detected with HRP-conjugated goat polyclonal antibody to mouse IgM and IgG, respectively (Abcam, Cambridge, MA, USA); IgG1 was detected with biotin-XX-goat anti-mouse IgG1 (Invitrogen, Carlsbad, CA, USA) and HRP-avidin D (Vector Laboratories, Burlingame, CA, USA); and IgG2c was detected with HRP-conjugated AffiniPure goat anti-mouse IgG Fcγ subclass 2c (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

    Techniques: Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay

    NK cell depletion enhances early B cell responses during chronic virus infection. WT B6 mice were treated with PK136 (Δ NK) or control antibody at days −2 and −3 before infection with Clone13. (A) The serum levels of anti-LCMV total IgG, IgM, IgG1, and IgG2c at day 8 pi were measured by ELISA. (B) An example of Fas and GL7 staining on gated B220 + cells (left) and the total number of GC B cells (right) within the spleen at day 8. (C) An example of CD138 and IgD staining on gated B220 + cells (left) and the total number of plasmablast B cells (right) within the spleen at day 8. The data represent 6–9 mice from 3 experiments. (D and E) In addition to NK cell depletion, some mice were treated with GK1.5 (Δ CD4) or control antibody at day −1 before infection and day 2 to remove CD4 + T cells. (D) The serum levels of anti-LCMV IgM (left) and total IgG (right) at day 8 pi. (E) The total number of GC (left) and plasmablast (right) B cells in the spleen at day 8 pi. The data represent 6 mice from 2 experiments. * P

    Journal: Journal of Leukocyte Biology

    Article Title: NK cells inhibit humoral immunity by reducing the abundance of CD4+ T follicular helper cells during a chronic virus infection

    doi: 10.1189/jlb.4HI1214-594R

    Figure Lengend Snippet: NK cell depletion enhances early B cell responses during chronic virus infection. WT B6 mice were treated with PK136 (Δ NK) or control antibody at days −2 and −3 before infection with Clone13. (A) The serum levels of anti-LCMV total IgG, IgM, IgG1, and IgG2c at day 8 pi were measured by ELISA. (B) An example of Fas and GL7 staining on gated B220 + cells (left) and the total number of GC B cells (right) within the spleen at day 8. (C) An example of CD138 and IgD staining on gated B220 + cells (left) and the total number of plasmablast B cells (right) within the spleen at day 8. The data represent 6–9 mice from 3 experiments. (D and E) In addition to NK cell depletion, some mice were treated with GK1.5 (Δ CD4) or control antibody at day −1 before infection and day 2 to remove CD4 + T cells. (D) The serum levels of anti-LCMV IgM (left) and total IgG (right) at day 8 pi. (E) The total number of GC (left) and plasmablast (right) B cells in the spleen at day 8 pi. The data represent 6 mice from 2 experiments. * P

    Article Snippet: The following antibodies were used for detection of antiviral antibodies: total IgM and IgG were detected with HRP-conjugated goat polyclonal antibody to mouse IgM and IgG, respectively (Abcam, Cambridge, MA, USA); IgG1 was detected with biotin-XX-goat anti-mouse IgG1 (Invitrogen, Carlsbad, CA, USA) and HRP-avidin D (Vector Laboratories, Burlingame, CA, USA); and IgG2c was detected with HRP-conjugated AffiniPure goat anti-mouse IgG Fcγ subclass 2c (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

    Techniques: Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay, Staining