goat antimouse igg  (Millipore)


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    Structured Review

    Millipore goat antimouse igg
    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat <t>antimouse</t> <t>IgG.</t> The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Goat Antimouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antimouse igg/product/Millipore
    Average 96 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    goat antimouse igg - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells"

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    Journal: Immunology

    doi: 10.1046/j.1365-2567.1999.00803.x

    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Figure Legend Snippet: Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.

    Techniques Used: Expressing, Infection, Incubation, Fluorescence, Negative Control

    (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).
    Figure Legend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).

    Techniques Used: Expressing, Infection, Incubation, Negative Control, Fluorescence

    (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.
    Figure Legend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.

    Techniques Used: Expressing, Infection, Incubation, Fluorescence

    2) Product Images from "Shape-coded silica nanotubes for multiplexed bioassay: rapid and reliable magnetic decoding protocols"

    Article Title: Shape-coded silica nanotubes for multiplexed bioassay: rapid and reliable magnetic decoding protocols

    Journal: Nanomedicine (London, England)

    doi: 10.2217/nnm.09.92

    Average fluorescence intensity of BMNT1 and -2 in assay 1 (dye-antirabbit IgG was used as an analyte), in assay 2 (both dye-antimouse IgG and dye-antirabbit IgG were analytes) and in cancer marker α-fetoprotein detection (α-fetoprotein
    Figure Legend Snippet: Average fluorescence intensity of BMNT1 and -2 in assay 1 (dye-antirabbit IgG was used as an analyte), in assay 2 (both dye-antimouse IgG and dye-antirabbit IgG were analytes) and in cancer marker α-fetoprotein detection (α-fetoprotein

    Techniques Used: Fluorescence, Marker

    3) Product Images from "Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells"

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    Journal: Immunology

    doi: 10.1046/j.1365-2567.1999.00803.x

    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Figure Legend Snippet: Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.

    Techniques Used: Expressing, Infection, Incubation, Fluorescence, Negative Control

    (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).
    Figure Legend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).

    Techniques Used: Expressing, Infection, Incubation, Negative Control, Fluorescence

    (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.
    Figure Legend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.

    Techniques Used: Expressing, Infection, Incubation, Fluorescence

    Related Articles

    Western Blot:

    Article Title: Synaptic Ultrastructure Might Be Involved in HCN1-Related BDNF mRNA in Withdrawal-Anxiety After Ethanol Dependence
    Article Snippet: .. The next day, the membrane was washed three times, 10 min each time, with PBS-T and incubated at room temperature with secondary antibodies, including anti-HCN1 [Peroxidase-Conjugated Affinipure Goat Anti-mouse IgG (H + L), 1:200] and anti-BDNF [Peroxidase-Conjugated Affinipure Goat Anti-Rabbit IgG (H + L), 1:200], and reacted with the Immobilon western chemiluminescent HRP substrate (WBKLS0500; Millipore, Bedford, MA, USA) for the color reaction. .. Then, the gray values of the immunoreactivity were quantified with ImageJ software.

    Incubation:

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells
    Article Snippet: .. The primary antibodies (w6/32, HC-10 and 3G6) were overlaid and incubated at +4° for 30 min. After two washes with the same buffer, PBMC were incubated with FITC-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min. After a further two washes, PBMC were centrifuged on slides and mounted with 90% glycerol in PBS containing 1 mg/ml phenylenediamine (Sigma Chemical Company). .. The samples were analysed using confocal microscopy (Leitz, Heidelberg, Germany) with 488 nm excitation wavelength.

    Article Title: Synaptic Ultrastructure Might Be Involved in HCN1-Related BDNF mRNA in Withdrawal-Anxiety After Ethanol Dependence
    Article Snippet: .. The next day, the membrane was washed three times, 10 min each time, with PBS-T and incubated at room temperature with secondary antibodies, including anti-HCN1 [Peroxidase-Conjugated Affinipure Goat Anti-mouse IgG (H + L), 1:200] and anti-BDNF [Peroxidase-Conjugated Affinipure Goat Anti-Rabbit IgG (H + L), 1:200], and reacted with the Immobilon western chemiluminescent HRP substrate (WBKLS0500; Millipore, Bedford, MA, USA) for the color reaction. .. Then, the gray values of the immunoreactivity were quantified with ImageJ software.

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells
    Article Snippet: .. Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO). .. Cell surface expression was analysed by fluorescence-activated cell sorter (FACScan®, Becton-Dickinson Immunocytometry Systems, Mountain View, CA).

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  • 96
    Millipore goat antimouse igg conjugated
    Mitochondrial CA (mCA) in the primary cultured PC derived from mouse brain microvessels. A, Cerebral PC stained for α-SMA are green , nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are blue . PC (1 × 10 4 ) were fixed on chamber slides with 4% paraformaldehyde in PBS, permeabilized, blocked with 0.3% Triton, 10% normal donkey serum (NDS) in PBS, and incubated with mouse antihuman α-SMA antibody at 10 μg/ml in 1% NDS in PBS. Secondary antibody was goat <t>antimouse</t> fluorescein isothiocyanate-conjugated <t>IgG</t> (1:200). After incubation and washing, slides were counterstained with DAPI (10 μg/ml) in mounting media. B, Transcripts of CA VA and VB in the PC and the brain. RNA isolated from PC was reversed transcribed and PCR amplified by mitochondrial CA VA-specific ( top ) and CA VB-specific ( bottom ) primers. Mouse brain RNA was used as a positive control. C, Mitochondrial CA VA and VB polypeptides in the PC and the brain. Proteins (50 μg) isolated from the PC were separated on polyacrylamide gels and probed with antimouse CA VA or VB antibody at 1:3000 dilution. Secondary antibody was goat antirabbit horseradish peroxidase (1:5000)-conjugated IgG. Proteins from the mouse brain were used as a positive control.
    Goat Antimouse Igg Conjugated, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antimouse igg conjugated/product/Millipore
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    goat antimouse igg conjugated - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    97
    Millipore fitc conjugated goat antimouse igg
    Mitochondrial CA (mCA) in the primary cultured PC derived from mouse brain microvessels. A, Cerebral PC stained for α-SMA are green , nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are blue . PC (1 × 10 4 ) were fixed on chamber slides with 4% paraformaldehyde in PBS, permeabilized, blocked with 0.3% Triton, 10% normal donkey serum (NDS) in PBS, and incubated with mouse antihuman α-SMA antibody at 10 μg/ml in 1% NDS in PBS. Secondary antibody was goat <t>antimouse</t> fluorescein isothiocyanate-conjugated <t>IgG</t> (1:200). After incubation and washing, slides were counterstained with DAPI (10 μg/ml) in mounting media. B, Transcripts of CA VA and VB in the PC and the brain. RNA isolated from PC was reversed transcribed and PCR amplified by mitochondrial CA VA-specific ( top ) and CA VB-specific ( bottom ) primers. Mouse brain RNA was used as a positive control. C, Mitochondrial CA VA and VB polypeptides in the PC and the brain. Proteins (50 μg) isolated from the PC were separated on polyacrylamide gels and probed with antimouse CA VA or VB antibody at 1:3000 dilution. Secondary antibody was goat antirabbit horseradish peroxidase (1:5000)-conjugated IgG. Proteins from the mouse brain were used as a positive control.
    Fitc Conjugated Goat Antimouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated goat antimouse igg/product/Millipore
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated goat antimouse igg - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    Mitochondrial CA (mCA) in the primary cultured PC derived from mouse brain microvessels. A, Cerebral PC stained for α-SMA are green , nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are blue . PC (1 × 10 4 ) were fixed on chamber slides with 4% paraformaldehyde in PBS, permeabilized, blocked with 0.3% Triton, 10% normal donkey serum (NDS) in PBS, and incubated with mouse antihuman α-SMA antibody at 10 μg/ml in 1% NDS in PBS. Secondary antibody was goat antimouse fluorescein isothiocyanate-conjugated IgG (1:200). After incubation and washing, slides were counterstained with DAPI (10 μg/ml) in mounting media. B, Transcripts of CA VA and VB in the PC and the brain. RNA isolated from PC was reversed transcribed and PCR amplified by mitochondrial CA VA-specific ( top ) and CA VB-specific ( bottom ) primers. Mouse brain RNA was used as a positive control. C, Mitochondrial CA VA and VB polypeptides in the PC and the brain. Proteins (50 μg) isolated from the PC were separated on polyacrylamide gels and probed with antimouse CA VA or VB antibody at 1:3000 dilution. Secondary antibody was goat antirabbit horseradish peroxidase (1:5000)-conjugated IgG. Proteins from the mouse brain were used as a positive control.

    Journal: Endocrinology

    Article Title: Topiramate Treatment Protects Blood-Brain Barrier Pericytes from Hyperglycemia-Induced Oxidative Damage in Diabetic Mice

    doi: 10.1210/en.2011-1638

    Figure Lengend Snippet: Mitochondrial CA (mCA) in the primary cultured PC derived from mouse brain microvessels. A, Cerebral PC stained for α-SMA are green , nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are blue . PC (1 × 10 4 ) were fixed on chamber slides with 4% paraformaldehyde in PBS, permeabilized, blocked with 0.3% Triton, 10% normal donkey serum (NDS) in PBS, and incubated with mouse antihuman α-SMA antibody at 10 μg/ml in 1% NDS in PBS. Secondary antibody was goat antimouse fluorescein isothiocyanate-conjugated IgG (1:200). After incubation and washing, slides were counterstained with DAPI (10 μg/ml) in mounting media. B, Transcripts of CA VA and VB in the PC and the brain. RNA isolated from PC was reversed transcribed and PCR amplified by mitochondrial CA VA-specific ( top ) and CA VB-specific ( bottom ) primers. Mouse brain RNA was used as a positive control. C, Mitochondrial CA VA and VB polypeptides in the PC and the brain. Proteins (50 μg) isolated from the PC were separated on polyacrylamide gels and probed with antimouse CA VA or VB antibody at 1:3000 dilution. Secondary antibody was goat antirabbit horseradish peroxidase (1:5000)-conjugated IgG. Proteins from the mouse brain were used as a positive control.

    Article Snippet: The antihuman FVIII antibody (MAB3440) and goat antimouse IgG conjugated with fluorescein isothiocyanate or Rhodamine Red were from Millipore (Billerica, MA).

    Techniques: Cell Culture, Derivative Assay, Staining, Incubation, Isolation, Polymerase Chain Reaction, Amplification, Positive Control