goat anti sox 2  (Abcam)

 
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  • 98
    Name:
    Goat F ab 2 Anti Rat IgG Fab 2 TR
    Description:

    Catalog Number:
    ab6267
    Price:
    None
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    Structured Review

    Abcam goat anti sox 2

    https://www.bioz.com/result/goat anti sox 2/product/Abcam
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti sox 2 - by Bioz Stars, 2020-07
    98/100 stars

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    Related Articles

    Western Blot:

    Article Title: MicroRNA‐138 Inhibits Osteogenic Differentiation and Mineralization of Human Dedifferentiated Chondrocytes by Regulating RhoC and the Actin Cytoskeleton
    Article Snippet: .. Western blots were performed with mouse anti‐β‐actin antibodies (Abcam ab6267; Abcam, Cambridge, UK) and either rabbit anti‐RUNX2 (Cell Signaling Technology #8486; Cell Signaling Technology, Inc, Danvers, MA, USA), rabbit anti‐osteocalcin (Abcam ab93876), or rabbit anti‐RhoC (Cell Signaling Technology #3430) primary antibodies. ..

    Article Title: Antiaging Gene Klotho Regulates Adrenal CYP11B2 Expression and Aldosterone Synthesis
    Article Snippet: .. Western blotting analysis was performed as we described previously., , , , Briefly, the membranes were blocked in 2%–3% BSA or 2.5%–5% milk in TBST for 2 hours and then incubated overnight (4°C) with primary antibodies against KL (15 μ g/ml; AF1819; in mouse adrenal glands of mice; R & D Systems), CYP11B2 (1:200; MAB6021 ; in mouse adrenal glands; EMD Millipore), MR (1:250; ab2774; Abcam, Inc.), SGK1 (1:100; sc-28338; Santa Cruz Biotechnology), NCC (1:1000; ab3553; EMD Millipore), ATP synthase- β (1:15,000; 612518; BD Transduction Laboratories Inc., Mississauga, ON, Canada), TNF- α (1 μ g/ml; ab6671; Abcam, Inc.), MCP-1 (1:500; 2029; Cell Signaling Technology, Danvers, MA), IL-6 (dilution 1:350; MABF41; EMD Millipore), osteopontin (1:1000; ab91665; Abcam, Inc.), and β -actin (1:7500; ab6267; Abcam, Inc.). .. Goat anti-mouse (1:2000; sc-2005; Santa Cruz Biotechnology) or goat anti-rabbit with HRP (1:10,000; sc-2004; Santa Cruz Biotechnology) was used as a secondary antibody and incubated for 1 hour at room temperature.

    Incubation:

    Article Title: Serotype b of Aggregatibacter actinomycetemcomitans triggers pro-inflammatory responses and amyloid beta secretion in hippocampal cells: a novel link between periodontitis and Alzheimer´s disease?
    Article Snippet: .. Subsequently, cells were incubated with the secondary anti-goat antibody (Alexa Fluor® 633, Abcam®) at 1:2.000 or anti-rabbit antibody (Alexa Fluor® 488) and anti-mouse (Alexa Fluor® 635, Thermo Fisher Scientific) both at 1:400 diluted in blocking solution and added to cells according to the protocol previously described [ ]. .. After 2 h incubation at room temperature, cells were washed 3 times with PBS and mounted in medium containing DAPI (Abcam®) in order to visualize the nuclei.

    Article Title: Amantadine attenuates sepsis-induced cognitive dysfunction possibly not through inhibiting toll-like receptor 2
    Article Snippet: .. The membranes were blocked with Protein-Free T20 Blocking Buffer (Cat. No. 37573, Thermo Scientific, Logan, UT) and incubated with the following primary antibodies overnight at 4 °C: rabbit monoclonal anti-TLR2 antibody (1:1000 dilution, Cat. No. ab108998, Abcam, Cambridge, MA), rabbit polyclonal anti-TLR4 antibody (1:1000 dilution, Cat. No. ab83444, Abcam, Cambridge, MA), mouse monoclonal anti-TLR9 antibody (1:500 dilution, Cat. No. ab12121, Abcam, Cambridge, MA), and mouse monoclonal anti-β-actin antibody (1:5000 dilution, Cat. No. ab6267, Abcam, Cambridge, MA). ..

    Article Title: Antiaging Gene Klotho Regulates Adrenal CYP11B2 Expression and Aldosterone Synthesis
    Article Snippet: .. Western blotting analysis was performed as we described previously., , , , Briefly, the membranes were blocked in 2%–3% BSA or 2.5%–5% milk in TBST for 2 hours and then incubated overnight (4°C) with primary antibodies against KL (15 μ g/ml; AF1819; in mouse adrenal glands of mice; R & D Systems), CYP11B2 (1:200; MAB6021 ; in mouse adrenal glands; EMD Millipore), MR (1:250; ab2774; Abcam, Inc.), SGK1 (1:100; sc-28338; Santa Cruz Biotechnology), NCC (1:1000; ab3553; EMD Millipore), ATP synthase- β (1:15,000; 612518; BD Transduction Laboratories Inc., Mississauga, ON, Canada), TNF- α (1 μ g/ml; ab6671; Abcam, Inc.), MCP-1 (1:500; 2029; Cell Signaling Technology, Danvers, MA), IL-6 (dilution 1:350; MABF41; EMD Millipore), osteopontin (1:1000; ab91665; Abcam, Inc.), and β -actin (1:7500; ab6267; Abcam, Inc.). .. Goat anti-mouse (1:2000; sc-2005; Santa Cruz Biotechnology) or goat anti-rabbit with HRP (1:10,000; sc-2004; Santa Cruz Biotechnology) was used as a secondary antibody and incubated for 1 hour at room temperature.

    Article Title: Heat Shock Protein Inspired Nanochaperones Restore Amyloid‐β Homeostasis for Preventative Therapy of Alzheimer's Disease, Heat Shock Protein Inspired Nanochaperones Restore Amyloid‐β Homeostasis for Preventative Therapy of Alzheimer's Disease
    Article Snippet: .. After incubation, the sections were washed by PBS and then incubated with secondary antibodies of goat antirabbit (Alexa 488, Abcam) or goat antirat (Alexa 594, Abcam). .. DAPI Fluoromount‐G (Southern Biotech) was used for the nuclear counterstaining.

    Blocking Assay:

    Article Title: Serotype b of Aggregatibacter actinomycetemcomitans triggers pro-inflammatory responses and amyloid beta secretion in hippocampal cells: a novel link between periodontitis and Alzheimer´s disease?
    Article Snippet: .. Subsequently, cells were incubated with the secondary anti-goat antibody (Alexa Fluor® 633, Abcam®) at 1:2.000 or anti-rabbit antibody (Alexa Fluor® 488) and anti-mouse (Alexa Fluor® 635, Thermo Fisher Scientific) both at 1:400 diluted in blocking solution and added to cells according to the protocol previously described [ ]. .. After 2 h incubation at room temperature, cells were washed 3 times with PBS and mounted in medium containing DAPI (Abcam®) in order to visualize the nuclei.

    Article Title: Amantadine attenuates sepsis-induced cognitive dysfunction possibly not through inhibiting toll-like receptor 2
    Article Snippet: .. The membranes were blocked with Protein-Free T20 Blocking Buffer (Cat. No. 37573, Thermo Scientific, Logan, UT) and incubated with the following primary antibodies overnight at 4 °C: rabbit monoclonal anti-TLR2 antibody (1:1000 dilution, Cat. No. ab108998, Abcam, Cambridge, MA), rabbit polyclonal anti-TLR4 antibody (1:1000 dilution, Cat. No. ab83444, Abcam, Cambridge, MA), mouse monoclonal anti-TLR9 antibody (1:500 dilution, Cat. No. ab12121, Abcam, Cambridge, MA), and mouse monoclonal anti-β-actin antibody (1:5000 dilution, Cat. No. ab6267, Abcam, Cambridge, MA). ..

    Mouse Assay:

    Article Title: Antiaging Gene Klotho Regulates Adrenal CYP11B2 Expression and Aldosterone Synthesis
    Article Snippet: .. Western blotting analysis was performed as we described previously., , , , Briefly, the membranes were blocked in 2%–3% BSA or 2.5%–5% milk in TBST for 2 hours and then incubated overnight (4°C) with primary antibodies against KL (15 μ g/ml; AF1819; in mouse adrenal glands of mice; R & D Systems), CYP11B2 (1:200; MAB6021 ; in mouse adrenal glands; EMD Millipore), MR (1:250; ab2774; Abcam, Inc.), SGK1 (1:100; sc-28338; Santa Cruz Biotechnology), NCC (1:1000; ab3553; EMD Millipore), ATP synthase- β (1:15,000; 612518; BD Transduction Laboratories Inc., Mississauga, ON, Canada), TNF- α (1 μ g/ml; ab6671; Abcam, Inc.), MCP-1 (1:500; 2029; Cell Signaling Technology, Danvers, MA), IL-6 (dilution 1:350; MABF41; EMD Millipore), osteopontin (1:1000; ab91665; Abcam, Inc.), and β -actin (1:7500; ab6267; Abcam, Inc.). .. Goat anti-mouse (1:2000; sc-2005; Santa Cruz Biotechnology) or goat anti-rabbit with HRP (1:10,000; sc-2004; Santa Cruz Biotechnology) was used as a secondary antibody and incubated for 1 hour at room temperature.

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  • 99
    Abcam goat anti sox 2 antibody
    Goat Anti Sox 2 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti sox 2 antibody/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti sox 2 antibody - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    98
    Abcam goat anti sox 2
    Goat Anti Sox 2, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti sox 2/product/Abcam
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti sox 2 - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

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