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Millipore goat anti rabbit
Goat Anti Rabbit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti rabbit/product/Millipore
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
goat anti rabbit - by Bioz Stars, 2021-06
93/100 stars

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Related Articles

Western Blot:

Article Title: Saponin Formosanin C-Induced Ferritinophagy and Ferroptosis in Human Hepatocellular Carcinoma Cells
Article Snippet: C11-BODIPY was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). .. Antibodies for Western blotting were purchased from the following vendors: rabbit monoclonal anti-CD71 (TFRC) and anti-ferritin heavy chain 1 (FTH1) antibodies, Cell Signaling Technology, Danvers, MA, USA; rabbit polyclonal anti-FPN/SLC40A1 antibody, Novus Biologicals, LLC, Littleton, CO, USA; rabbit polyclonal anti-ARA70 (NCOA4) antibody, Bethyl Laboratories, Inc., Montgomery, TX, USA; rabbit polyclonal anti-LC3B antibody, Abcam, Cambridge Science Park, Cambridge, UK; rabbit polyclonal anti-GAPDH antibody, GeneTex, Irvine, CA, USA; mouse monoclonal anti-β-actin antibody, Sigma; goat anti-rabbit (H+L) and goat anti-mouse IgM+IgG+IgA (H+L) HRP conjugate antibodies, Millipore Corp., Billerica, MA, USA. .. Cell Culture Human HCC cell lines Hep3B and HepG2 from American Type Culture Collection (Rockville, MD, USA) were grown at 37 °C in Dulbecco’s modified Eagle medium (GIBCO BRL, Gaithersburg, MD, USA) supplemented with penicillin and streptomycin as well as 10% fetal bovine serum (GIBCO) in a humidified atmosphere containing 5% CO2.

Binding Assay:

Article Title: Cathepsin K-Targeted Sub-Micron Particles for Regenerative Repair of Vascular Elastic Matrix
Article Snippet: .. A fluorescein-tagged goat anti-rabbit secondary antibody (Chemicon, Temecula, CA), was used to qualitatively determine the relative binding of the cathepsin K pAb on the surface of the SMPs. ..

Quantitative RT-PCR:

Article Title: Flow-induced prostaglandin E2 release regulates Na and K transport in the collecting duct
Article Snippet: Inhibitors were as follows: 10 μM U0126 (Calbiochem, San Diego, CA), 10 μM SB203580 (Cayman Chemical), 30 μM SP600125 ( ) (Calbiochem), 125 μM indomethacin ( ) (Sigma), 20 μM BAPTA-AM (Invitrogen), 100 nM SC560 (Cayman Chemical), and 1 μM (Cayman Chemical). .. Primers for qRT-PCR (Applied Biosciences) were as follows: COX-1 (Mm00477214_m1), COX-2 (Mm00478374_m1), Ptges-1 (Mm00452105_m1), and GAPDH (Mm99999915_g1); and antibodies were as follows: polyclonal anti-phospho-Ser505-cPLA2 (1:500; Cell Signaling), polyclonal cPLA2 (1:500; Cell Signaling), rabbit polyclonal anti-phospho-p38 (1:1,000; Cell Signaling), rabbit polyclonal anti-COX-2 (1:1,000; Cayman Chemical), mouse monoclonal anti-actin (1:1,000; Cell Signaling), mouse monoclonal anti-GAPDH (1:4,000; Santa Cruz Biotechnology), and goat anti-rabbit conjugated to horseradish peroxidase (1:5,000; Sigma). ..

Antibody Labeling:

Article Title: Analysis of Borrelia burgdorferivlsE Gene Expression and Recombination in the Tick Vector
Article Snippet: On Western blots, this antibody reacts with the VlsE from spirochetes of the B. burgdorferi sensu stricto (including B31), B. garinii , and B. afzelii genospecies ( ). .. Fluorescein-labeled goat antibody against rabbit immunoglobulin was obtained from Sigma and used in indirect fluorescent antibody labeling (IFA) experiments. .. Fluorescein-labeled anti- B. burgdorferi antibody was purchased from Kirkegaard & Perry Laboratories (Gaithersburg, Md.) and used in direct fluorescent antibody (DFA) labeling experiments.

Immunofluorescence:

Article Title: Analysis of Borrelia burgdorferivlsE Gene Expression and Recombination in the Tick Vector
Article Snippet: On Western blots, this antibody reacts with the VlsE from spirochetes of the B. burgdorferi sensu stricto (including B31), B. garinii , and B. afzelii genospecies ( ). .. Fluorescein-labeled goat antibody against rabbit immunoglobulin was obtained from Sigma and used in indirect fluorescent antibody labeling (IFA) experiments. .. Fluorescein-labeled anti- B. burgdorferi antibody was purchased from Kirkegaard & Perry Laboratories (Gaithersburg, Md.) and used in direct fluorescent antibody (DFA) labeling experiments.

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  • 86
    Millipore cy3 labeled goat anti rabbit
    Immunohistochemical detection of Kv4.2W362F-FLAG in transfected SCG neurons. Isolated SCG neurons, transfected with EGFP alone ( A ) or with Kv4.2W362F-FLAG and EGFP ( B ) using the gene gun, were fixed and stained 24 hr later (see Materials and Methods). A , B , EGFP fluorescence ( left panels ) and <t>Cy3</t> fluorescence ( right panels ) images of the same field. Anti-FLAG staining is only evident in cultures transfected with Kv4.2W362F-FLAG expression (compare right panels in A and B ). In addition, EGFP expression correlates with Kv4.2W362F (compare left and right panels in B ). Scale bar, 50 μm.
    Cy3 Labeled Goat Anti Rabbit, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 labeled goat anti rabbit/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 labeled goat anti rabbit - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Millipore rabbit anti glur1 antibody
    SP shRNA-transfected neurons demonstrate reduced accumulation of <t>GluR1-clusters</t> in spine heads after the induction of LTP. A–E , A transient activation of synaptic NMDA-Rs (cLTP) causes an increase in the number of SP(+) spines within 90 min (untreated controls: n = 4 cultures, 16 segments, 778 spines; APV: n = 4 cultures, 16 segments, 531 spines; cLTP: n = 3 cultures, 12 segments, 353 spines). Arrowheads point at SP(+) spines, and the arrows highlight endogenous SP in the dendrite. Scale bar, 2 μm. B–E , In APV-treated cultures, a decrease in spine density ( B ; p
    Rabbit Anti Glur1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti glur1 antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti glur1 antibody - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Millipore goat anti rabbit il 1β
    PI3-K is required for resveratrol-inhibited the production of NO, PGE2, TNF-α and <t>IL-1β</t> induced by LPS in RAW 264.7 macrophage cells. Approximately 1×10 6 cells/ml were seeded in six-well plates and incubated until 80% confluency. Cells were pre-treated with resveratrol (1, 5, and 10 µM) for 1 h in the absence or presence of wortmannin (1 µM), then exposed to LPS (5 µg/ml) for 12 h. The concentrations of NO (A), PGE2 (B), TNF-α (C) and IL-1β (D) were measured as described in the methods . The values shown are mean ± SEM of data from three independent experiments. # Significant compared with control alone, p
    Goat Anti Rabbit Il 1β, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit il 1β/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit il 1β - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Millipore goat anti rabbit igg hrp
    IL-37 impairs inflammasome activation in mice with aspergillosis. C57BL/6 mice were infected intranasally with A. fumigatus and treated intraperitoneally with recombinant IL-37 precursor, at the indicated doses, 96, 48 and 1 hour before the infection. ( A ) NLRP3 expression in the lung by immunofluorescence staining with anti-CIAS1/Nlrp3 antibody. In the insets, positive staining of epithelial cells. Nuclei were counterstained with DAPI. Scale bars, 100 µm. ( B, E ) Gene expression on total lung cells by RT-PCR. ( C ) Cytokine production (ELISA) on lung homogenates. ( D ) Immunoblot analysis on whole lung lysates of IL-1β and Caspase 1 using rabbit specific antibodies and rabbit anti-actin. Goat anti-rabbit <t>IgG-HRP</t> were used as secondary antibody. Corresponding pixel density ratio was normalized against actin. Assays were done a day after the infection. Data are representative (immunoblotting) or pooled from three experiments. *P
    Goat Anti Rabbit Igg Hrp, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg hrp/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg hrp - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Immunohistochemical detection of Kv4.2W362F-FLAG in transfected SCG neurons. Isolated SCG neurons, transfected with EGFP alone ( A ) or with Kv4.2W362F-FLAG and EGFP ( B ) using the gene gun, were fixed and stained 24 hr later (see Materials and Methods). A , B , EGFP fluorescence ( left panels ) and Cy3 fluorescence ( right panels ) images of the same field. Anti-FLAG staining is only evident in cultures transfected with Kv4.2W362F-FLAG expression (compare right panels in A and B ). In addition, EGFP expression correlates with Kv4.2W362F (compare left and right panels in B ). Scale bar, 50 μm.

    Journal: The Journal of Neuroscience

    Article Title: Elimination of the Fast Transient in Superior Cervical Ganglion Neurons with Expression of KV4.2W362F: Molecular Dissection ofIA

    doi: 10.1523/JNEUROSCI.20-14-05191.2000

    Figure Lengend Snippet: Immunohistochemical detection of Kv4.2W362F-FLAG in transfected SCG neurons. Isolated SCG neurons, transfected with EGFP alone ( A ) or with Kv4.2W362F-FLAG and EGFP ( B ) using the gene gun, were fixed and stained 24 hr later (see Materials and Methods). A , B , EGFP fluorescence ( left panels ) and Cy3 fluorescence ( right panels ) images of the same field. Anti-FLAG staining is only evident in cultures transfected with Kv4.2W362F-FLAG expression (compare right panels in A and B ). In addition, EGFP expression correlates with Kv4.2W362F (compare left and right panels in B ). Scale bar, 50 μm.

    Article Snippet: After washing, cultures were incubated with a Cy3-labeled goat anti-rabbit (or rabbit anti-mouse) IgG secondary antibody (Chemicon, Temecula, CA).

    Techniques: Immunohistochemistry, Transfection, Isolation, Staining, Fluorescence, Expressing

    SP shRNA-transfected neurons demonstrate reduced accumulation of GluR1-clusters in spine heads after the induction of LTP. A–E , A transient activation of synaptic NMDA-Rs (cLTP) causes an increase in the number of SP(+) spines within 90 min (untreated controls: n = 4 cultures, 16 segments, 778 spines; APV: n = 4 cultures, 16 segments, 531 spines; cLTP: n = 3 cultures, 12 segments, 353 spines). Arrowheads point at SP(+) spines, and the arrows highlight endogenous SP in the dendrite. Scale bar, 2 μm. B–E , In APV-treated cultures, a decrease in spine density ( B ; p

    Journal: The Journal of Neuroscience

    Article Title: Synaptopodin Regulates Plasticity of Dendritic Spines in Hippocampal Neurons

    doi: 10.1523/JNEUROSCI.5528-08.2009

    Figure Lengend Snippet: SP shRNA-transfected neurons demonstrate reduced accumulation of GluR1-clusters in spine heads after the induction of LTP. A–E , A transient activation of synaptic NMDA-Rs (cLTP) causes an increase in the number of SP(+) spines within 90 min (untreated controls: n = 4 cultures, 16 segments, 778 spines; APV: n = 4 cultures, 16 segments, 531 spines; cLTP: n = 3 cultures, 12 segments, 353 spines). Arrowheads point at SP(+) spines, and the arrows highlight endogenous SP in the dendrite. Scale bar, 2 μm. B–E , In APV-treated cultures, a decrease in spine density ( B ; p

    Article Snippet: Cultures were incubated for 1 h with 10% normal goat serum (NGS) in 0.1% Triton X-100 containing PBS to reduce unspecific staining and subsequently incubated for 24 h at 4°C in rabbit anti-SP antibody (SE-19, Sigma; 1:1000, 10% bovine serum albumin, 0.1% Triton X-100 in PBS), in rabbit anti-GluR1 antibody (Millipore; AB 1504, 1:200, 10% goat serum in PBS), or rabbit anti-RYR1 antibody (gift from Dr. Shoshan-Barmatz BGU, Israel, 1:250, 10% goat serum in PBS) ( ).

    Techniques: shRNA, Transfection, Activation Assay

    PI3-K is required for resveratrol-inhibited the production of NO, PGE2, TNF-α and IL-1β induced by LPS in RAW 264.7 macrophage cells. Approximately 1×10 6 cells/ml were seeded in six-well plates and incubated until 80% confluency. Cells were pre-treated with resveratrol (1, 5, and 10 µM) for 1 h in the absence or presence of wortmannin (1 µM), then exposed to LPS (5 µg/ml) for 12 h. The concentrations of NO (A), PGE2 (B), TNF-α (C) and IL-1β (D) were measured as described in the methods . The values shown are mean ± SEM of data from three independent experiments. # Significant compared with control alone, p

    Journal: PLoS ONE

    Article Title: Resveratrol Inhibits LPS-Induced MAPKs Activation via Activation of the Phosphatidylinositol 3-Kinase Pathway in Murine RAW 264.7 Macrophage Cells

    doi: 10.1371/journal.pone.0044107

    Figure Lengend Snippet: PI3-K is required for resveratrol-inhibited the production of NO, PGE2, TNF-α and IL-1β induced by LPS in RAW 264.7 macrophage cells. Approximately 1×10 6 cells/ml were seeded in six-well plates and incubated until 80% confluency. Cells were pre-treated with resveratrol (1, 5, and 10 µM) for 1 h in the absence or presence of wortmannin (1 µM), then exposed to LPS (5 µg/ml) for 12 h. The concentrations of NO (A), PGE2 (B), TNF-α (C) and IL-1β (D) were measured as described in the methods . The values shown are mean ± SEM of data from three independent experiments. # Significant compared with control alone, p

    Article Snippet: RAW 264.7 cells were incubated with DAPI (dilution 1∶50,000; Sigma) plus goat anti-mouse iNOS (dilution 1∶500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-mouse COX-2 (dilution 1∶500; Santa Cruz Biotechnology), goat anti-rabbit TNF-α polyclonal antibody (dilution 1∶500; Chemicon, Temecula, CA,USA), goat anti-rabbit IL-1β (dilution 1∶500; Chemicon).

    Techniques: Incubation

    PI3-K is involved in resveratrol-attenuated the production of the proinflammatory cytokine IL-1β at the transcriptional and translational levels in RAW 264.7 macrophage cells. Panel A shows the immunofluorenscence images for protein expression of IL-1β and Panel B shows the corresponding mRNA data. The relative mRNA level was quantified by scanning densitometry and normalized to β-actin mRNA. Note the up-regulated protein and mRNA expression of IL-1β by LPS is suppressed by different concentrations of resveratrol; however, in cells pretreated with PI3-K inhibitor wortmannin, the suppressive effect of resveratrol is abrogated. The values shown are mean ± SEM of data from three independent experiments. # Significant compared with control alone, p

    Journal: PLoS ONE

    Article Title: Resveratrol Inhibits LPS-Induced MAPKs Activation via Activation of the Phosphatidylinositol 3-Kinase Pathway in Murine RAW 264.7 Macrophage Cells

    doi: 10.1371/journal.pone.0044107

    Figure Lengend Snippet: PI3-K is involved in resveratrol-attenuated the production of the proinflammatory cytokine IL-1β at the transcriptional and translational levels in RAW 264.7 macrophage cells. Panel A shows the immunofluorenscence images for protein expression of IL-1β and Panel B shows the corresponding mRNA data. The relative mRNA level was quantified by scanning densitometry and normalized to β-actin mRNA. Note the up-regulated protein and mRNA expression of IL-1β by LPS is suppressed by different concentrations of resveratrol; however, in cells pretreated with PI3-K inhibitor wortmannin, the suppressive effect of resveratrol is abrogated. The values shown are mean ± SEM of data from three independent experiments. # Significant compared with control alone, p

    Article Snippet: RAW 264.7 cells were incubated with DAPI (dilution 1∶50,000; Sigma) plus goat anti-mouse iNOS (dilution 1∶500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), goat anti-mouse COX-2 (dilution 1∶500; Santa Cruz Biotechnology), goat anti-rabbit TNF-α polyclonal antibody (dilution 1∶500; Chemicon, Temecula, CA,USA), goat anti-rabbit IL-1β (dilution 1∶500; Chemicon).

    Techniques: Expressing

    IL-37 impairs inflammasome activation in mice with aspergillosis. C57BL/6 mice were infected intranasally with A. fumigatus and treated intraperitoneally with recombinant IL-37 precursor, at the indicated doses, 96, 48 and 1 hour before the infection. ( A ) NLRP3 expression in the lung by immunofluorescence staining with anti-CIAS1/Nlrp3 antibody. In the insets, positive staining of epithelial cells. Nuclei were counterstained with DAPI. Scale bars, 100 µm. ( B, E ) Gene expression on total lung cells by RT-PCR. ( C ) Cytokine production (ELISA) on lung homogenates. ( D ) Immunoblot analysis on whole lung lysates of IL-1β and Caspase 1 using rabbit specific antibodies and rabbit anti-actin. Goat anti-rabbit IgG-HRP were used as secondary antibody. Corresponding pixel density ratio was normalized against actin. Assays were done a day after the infection. Data are representative (immunoblotting) or pooled from three experiments. *P

    Journal: PLoS Pathogens

    Article Title: IL-37 Inhibits Inflammasome Activation and Disease Severity in Murine Aspergillosis

    doi: 10.1371/journal.ppat.1004462

    Figure Lengend Snippet: IL-37 impairs inflammasome activation in mice with aspergillosis. C57BL/6 mice were infected intranasally with A. fumigatus and treated intraperitoneally with recombinant IL-37 precursor, at the indicated doses, 96, 48 and 1 hour before the infection. ( A ) NLRP3 expression in the lung by immunofluorescence staining with anti-CIAS1/Nlrp3 antibody. In the insets, positive staining of epithelial cells. Nuclei were counterstained with DAPI. Scale bars, 100 µm. ( B, E ) Gene expression on total lung cells by RT-PCR. ( C ) Cytokine production (ELISA) on lung homogenates. ( D ) Immunoblot analysis on whole lung lysates of IL-1β and Caspase 1 using rabbit specific antibodies and rabbit anti-actin. Goat anti-rabbit IgG-HRP were used as secondary antibody. Corresponding pixel density ratio was normalized against actin. Assays were done a day after the infection. Data are representative (immunoblotting) or pooled from three experiments. *P

    Article Snippet: Goat anti-rabbit IgG-HRP (Sigma-Aldrich) was used as secondary antibodies.

    Techniques: Activation Assay, Mouse Assay, Infection, Recombinant, Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay