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Abcam goat anti rabbit igg biotin conjugated
Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific <t>anti-RrgB</t> antibodies (1∶400 dilution) and FITC anti-mouse <t>IgG</t> secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).
Goat Anti Rabbit Igg Biotin Conjugated, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern"

Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

Journal: PLoS ONE

doi: 10.1371/journal.pone.0021269

Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).
Figure Legend Snippet: Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).

Techniques Used: Expressing, Labeling, Flow Cytometry, Cytometry, FACS, Microarray, In Vitro, Hybridization

Pilus components display a biphasic expression pattern. A) TIGR4 bacteria were labeled with anti-RrgA, RrgB clade I, RrgC, BgaA (beta-galactosidase), PhtA (pneumococcal histidine triad protein A) or PspC (pneumococcal surface protein C, also known as CbpA,) primary antibodies (1∶400 dilution), and with FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Bacterial staining was analyzed by flow cytometry (FACS-Calibur). Sera of mice immunized with PBS were used as negative control. B,C) TIGR4 bacteria were processed for immunofluorescence, stained with mouse anti-RrgB antibodies (1∶2000 dilution) (red) and with S. pneumoniae anti-capsular antibodies (Omniserum 1∶2000 dilution) (green). Imaging was performed with a confocal microscope. Scale bar is 4 µm in panel B and 1 µm in panel C.
Figure Legend Snippet: Pilus components display a biphasic expression pattern. A) TIGR4 bacteria were labeled with anti-RrgA, RrgB clade I, RrgC, BgaA (beta-galactosidase), PhtA (pneumococcal histidine triad protein A) or PspC (pneumococcal surface protein C, also known as CbpA,) primary antibodies (1∶400 dilution), and with FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Bacterial staining was analyzed by flow cytometry (FACS-Calibur). Sera of mice immunized with PBS were used as negative control. B,C) TIGR4 bacteria were processed for immunofluorescence, stained with mouse anti-RrgB antibodies (1∶2000 dilution) (red) and with S. pneumoniae anti-capsular antibodies (Omniserum 1∶2000 dilution) (green). Imaging was performed with a confocal microscope. Scale bar is 4 µm in panel B and 1 µm in panel C.

Techniques Used: Expressing, Labeling, Staining, Flow Cytometry, Cytometry, FACS, Mouse Assay, Negative Control, Immunofluorescence, Imaging, Microscopy

Pilus expression ratio is not correlated with serotype, genotype or clade type. Bacteria containing either PI-1 clade I (A), clade II (B) or clade III (C), were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur).
Figure Legend Snippet: Pilus expression ratio is not correlated with serotype, genotype or clade type. Bacteria containing either PI-1 clade I (A), clade II (B) or clade III (C), were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur).

Techniques Used: Expressing, Labeling, Flow Cytometry, Cytometry, FACS

Related Articles

Incubation:

Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern
Article Snippet: .. Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies. .. Labelled bacteria were then incubated with Sepharose magnetic beads coated with streptavidin (Invitrogen) for 1 h at 4°C.

Expressing:

Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern
Article Snippet: .. Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies. .. Labelled bacteria were then incubated with Sepharose magnetic beads coated with streptavidin (Invitrogen) for 1 h at 4°C.

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    Abcam biotin conjugated goat anti rabbit igg h l
    SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels:  a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc;  b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H  L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)
    Biotin Conjugated Goat Anti Rabbit Igg H L, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 32 article reviews
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    biotin conjugated goat anti rabbit igg h l - by Bioz Stars, 2020-09
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    SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels:  a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc;  b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H  L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)

    Journal: BMC Biotechnology

    Article Title: Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene – methyltetrazine reactions

    doi: 10.1186/s12896-017-0381-2

    Figure Lengend Snippet: SDS-PAGE analysis of co-immunoprecipitation experiments under non-reducing condition. Panels: a , receptor-mediated co-immunoprecipitation using hFasRECD-Fc; b , antibody-mediated co-immunoprecipitation using biotin conjugated goat anti-rabbit IgG H L. Lanes (in both panels): M, molecular-weight size markers; 1 and 2, Avidin-MTZ alone; 3 and 4, Avidin-hFasLECD conjugate; 5 and 6, hFasLECD-TCO alone; 7 and 8, rFab’-hFasLECD conjugate (the peak 1 fraction); 9 and 10, rFab’-hFasLECD conjugate (the combined fractions of peaks 2 and 3); 11 and 12, rFab’-MTZ alone; 1, 3, 5, 7, 9 and 11, examined samples; 2, 4, 6, 8, 10 and 12, precipitated materials (IP)

    Article Snippet: Biotin conjugated goat anti-rabbit IgG H & L (ab207995) and ATTO495-Biotin were from Abcam Co. and ATTO-TEC GmbH, respectively.

    Techniques: SDS Page, Immunoprecipitation, Molecular Weight, Avidin-Biotin Assay

    Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).

    Journal: PLoS ONE

    Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

    doi: 10.1371/journal.pone.0021269

    Figure Lengend Snippet: Pilus-1 expression is regulated at the transcriptional level. A) High and low pilus expressing sub-populations of strains TIGR4 (Clade I), 19F Taiwan 14 (Clade I), OREP4 (Clade I), 6B Finland 14 (Clade II) and 35B SME 15 (Clade III) were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur). B) Schematic representation of PI-1. C) Log 2 ratio values indicating the PI-1 genes differential expression in High vs. Low pilus expressing sub-populations in the five above mentioned strains, as measured by spotted DNA microarray analysis. The data are measures of relative gene expression during in vitro growth in liquid cultures. The values reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent standard deviations). D) Absolute gene expression levels of PI-1 genes measured for TIGR4 high and low pilus expressing sub-populations by microarray hybridization. Absolute expression levels reported for each gene are the mean of all the spots and their replicates within the array and of two independent experiments (bars represent the obtained standard deviations).

    Article Snippet: Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies.

    Techniques: Expressing, Labeling, Flow Cytometry, Cytometry, FACS, Microarray, In Vitro, Hybridization

    Pilus components display a biphasic expression pattern. A) TIGR4 bacteria were labeled with anti-RrgA, RrgB clade I, RrgC, BgaA (beta-galactosidase), PhtA (pneumococcal histidine triad protein A) or PspC (pneumococcal surface protein C, also known as CbpA,) primary antibodies (1∶400 dilution), and with FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Bacterial staining was analyzed by flow cytometry (FACS-Calibur). Sera of mice immunized with PBS were used as negative control. B,C) TIGR4 bacteria were processed for immunofluorescence, stained with mouse anti-RrgB antibodies (1∶2000 dilution) (red) and with S. pneumoniae anti-capsular antibodies (Omniserum 1∶2000 dilution) (green). Imaging was performed with a confocal microscope. Scale bar is 4 µm in panel B and 1 µm in panel C.

    Journal: PLoS ONE

    Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

    doi: 10.1371/journal.pone.0021269

    Figure Lengend Snippet: Pilus components display a biphasic expression pattern. A) TIGR4 bacteria were labeled with anti-RrgA, RrgB clade I, RrgC, BgaA (beta-galactosidase), PhtA (pneumococcal histidine triad protein A) or PspC (pneumococcal surface protein C, also known as CbpA,) primary antibodies (1∶400 dilution), and with FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Bacterial staining was analyzed by flow cytometry (FACS-Calibur). Sera of mice immunized with PBS were used as negative control. B,C) TIGR4 bacteria were processed for immunofluorescence, stained with mouse anti-RrgB antibodies (1∶2000 dilution) (red) and with S. pneumoniae anti-capsular antibodies (Omniserum 1∶2000 dilution) (green). Imaging was performed with a confocal microscope. Scale bar is 4 µm in panel B and 1 µm in panel C.

    Article Snippet: Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies.

    Techniques: Expressing, Labeling, Staining, Flow Cytometry, Cytometry, FACS, Mouse Assay, Negative Control, Immunofluorescence, Imaging, Microscopy

    Pilus expression ratio is not correlated with serotype, genotype or clade type. Bacteria containing either PI-1 clade I (A), clade II (B) or clade III (C), were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur).

    Journal: PLoS ONE

    Article Title: The Streptococcus pneumoniae Pilus-1 Displays a Biphasic Expression Pattern

    doi: 10.1371/journal.pone.0021269

    Figure Lengend Snippet: Pilus expression ratio is not correlated with serotype, genotype or clade type. Bacteria containing either PI-1 clade I (A), clade II (B) or clade III (C), were labeled with clade specific anti-RrgB antibodies (1∶400 dilution) and FITC anti-mouse IgG secondary antibodies (1∶100 dilution). Pilus-1 expression was then analyzed by flow cytometry (FACS-Calibur).

    Article Snippet: Depletion of RrgB positive bacteria TIGR4 low pilus expressing bacteria were incubated with rabbit anti-RrgB antibodies (1∶400 dilution) and then with goat anti-rabbit IgG biotin conjugated (Abcam, 1∶1000 dilution) antibodies.

    Techniques: Expressing, Labeling, Flow Cytometry, Cytometry, FACS