goat anti rabbit horseradish peroxidase conjugate  (Thermo Fisher)


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    Name:
    Goat anti Rabbit IgG H L Poly HRP Secondary Antibody
    Description:
    Goat anti Rabbit IgG H L Poly HRP Secondary Antibody for Western Blot IHC ELISA
    Catalog Number:
    32260
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher goat anti rabbit horseradish peroxidase conjugate
    Compound 7 inhibits the MIF–sCD74 binding as determined by an ELISA assay. Binding of MBP–sCD74 to MIF-coated ELISA plates was detected using a <t>rabbit</t> <t>anti-CD74</t> antibody as the primary and a <t>goat</t> anti-rabbit <t>horseradish</t> <t>peroxidase</t> <t>conjugate</t> as the secondary antibody. Data are displayed as mean ± SD ( n = 3).
    Goat anti Rabbit IgG H L Poly HRP Secondary Antibody for Western Blot IHC ELISA
    https://www.bioz.com/result/goat anti rabbit horseradish peroxidase conjugate/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit horseradish peroxidase conjugate - by Bioz Stars, 2021-04
    97/100 stars

    Images

    1) Product Images from "7-Hydroxycoumarins Are Affinity-Based Fluorescent Probes for Competitive Binding Studies of Macrophage Migration Inhibitory Factor"

    Article Title: 7-Hydroxycoumarins Are Affinity-Based Fluorescent Probes for Competitive Binding Studies of Macrophage Migration Inhibitory Factor

    Journal: Journal of Medicinal Chemistry

    doi: 10.1021/acs.jmedchem.0c01160

    Compound 7 inhibits the MIF–sCD74 binding as determined by an ELISA assay. Binding of MBP–sCD74 to MIF-coated ELISA plates was detected using a rabbit anti-CD74 antibody as the primary and a goat anti-rabbit horseradish peroxidase conjugate as the secondary antibody. Data are displayed as mean ± SD ( n = 3).
    Figure Legend Snippet: Compound 7 inhibits the MIF–sCD74 binding as determined by an ELISA assay. Binding of MBP–sCD74 to MIF-coated ELISA plates was detected using a rabbit anti-CD74 antibody as the primary and a goat anti-rabbit horseradish peroxidase conjugate as the secondary antibody. Data are displayed as mean ± SD ( n = 3).

    Techniques Used: Binding Assay, Enzyme-linked Immunosorbent Assay

    Related Articles

    Incubation:

    Article Title: A phage-displayed peptide recognizing porcine aminopeptidase N is a potent small molecule inhibitor of PEDV entry
    Article Snippet: After three washes with PBS, membranes were sliced and incubated with phage H (1×1011 PFU), anti-PEDV S polyclonal antibody (1:1000 in PBS), M13 phage library (1×1011 PFU), and control phage bearing either the peptides SVSVGMKPSPRP or MSCNDTLCLLPN. .. The membranes were then washed with PBS and successively incubated with anti-M13 polyclonal antibody (Abcam, 1:600 in PBS) and horseradish peroxidase HRP-conjugated goat anti-rabbit IgG (1:1500 in PBS) at room temperature for 1 h. Protein bands were visualized using 3,3׳-diaminodbenzidine (DAB, The Thermo Scientific) .. Temperature shift assays To examine the effect of temperature on the binding of virus to the cells, four experiments were performed.

    Article Title: Aberrant Splicing in Transgenes Containing Introns, Exons, and V5 Epitopes: Lessons from Developing an FSHD Mouse Model Expressing a D4Z4 Repeat with Flanking Genomic Sequences
    Article Snippet: All constructs (DUX4.V5, DUX4.HOX1.V5 and DUX4-55aa) were transcribed from the CMV promoter. .. Following separation of proteins on SDS-PAGE and transfer to PVDF, membranes were incubated with rabbit polyclonal anti-DUX4 primary antibodies (1:250; Biorybt), followed by HRP-coupled goat anti-rabbit secondary antibodies (1:100,000; Jackson Immunochemicals), and developed on film with chemiluminescence substrate (Pierce). .. In silico analyses In silico analysis was performed by the Research Data and Computing Services (RDC) at the Research Institute at Nationwide Children’s Hospital.

    Article Title: 7-Hydroxycoumarins Are Affinity-Based Fluorescent Probes for Competitive Binding Studies of Macrophage Migration Inhibitory Factor
    Article Snippet: PBS buffer (100 μL) without sCD74 was used as control to exclude the nonspecific binding of anti-CD74 pAb. .. After washing, the wells were incubated with 100 μL of a rabbit anti-CD74 pAb solution (1:1000 dilution in PBS, 0.2% BSA) (Sinobiological, The Netherlands) at room temperature for 30 min. After removing the anti-CD74 solution and washing, a solution of 100 μL of goat anti-rabbit horseradish peroxidase conjugate (1:1000 dilution in PBS, 0.2% BSA) (Life Technologies, The Netherlands) was added and incubated at room temperature for 30 min. After washing, binding was visualized by conversion of 100 μL of aqueous tetramethylbenzydine (TMB) solution (Sigma Aldrich, The Netherlands), which was quenched with an aqueous 1 N H2 SO4 solution (100 μL). ..

    Article Title: Relevance of differential immunogenicity of human and mouse recombinant desmoglein-3 for the induction of Acantholytic autoantibodies in mice
    Article Snippet: .. Blots were treated for 1 h with a blocking buffer (5% non-fat dry milk in 200 m m NaCl with 0·1% Tween-20), and then incubated for 1 h each with the following reagents in succession with washing between each step: 1 : 2000 diluted rabbit anti-hdsg3 antibody [ ], and 1 : 3000 diluted HRP-conjugated goat antirabbit IgG (Caltag Laboratories, San Francisco, CA, USA). ..

    SDS Page:

    Article Title: Aberrant Splicing in Transgenes Containing Introns, Exons, and V5 Epitopes: Lessons from Developing an FSHD Mouse Model Expressing a D4Z4 Repeat with Flanking Genomic Sequences
    Article Snippet: All constructs (DUX4.V5, DUX4.HOX1.V5 and DUX4-55aa) were transcribed from the CMV promoter. .. Following separation of proteins on SDS-PAGE and transfer to PVDF, membranes were incubated with rabbit polyclonal anti-DUX4 primary antibodies (1:250; Biorybt), followed by HRP-coupled goat anti-rabbit secondary antibodies (1:100,000; Jackson Immunochemicals), and developed on film with chemiluminescence substrate (Pierce). .. In silico analyses In silico analysis was performed by the Research Data and Computing Services (RDC) at the Research Institute at Nationwide Children’s Hospital.

    Binding Assay:

    Article Title: 7-Hydroxycoumarins Are Affinity-Based Fluorescent Probes for Competitive Binding Studies of Macrophage Migration Inhibitory Factor
    Article Snippet: PBS buffer (100 μL) without sCD74 was used as control to exclude the nonspecific binding of anti-CD74 pAb. .. After washing, the wells were incubated with 100 μL of a rabbit anti-CD74 pAb solution (1:1000 dilution in PBS, 0.2% BSA) (Sinobiological, The Netherlands) at room temperature for 30 min. After removing the anti-CD74 solution and washing, a solution of 100 μL of goat anti-rabbit horseradish peroxidase conjugate (1:1000 dilution in PBS, 0.2% BSA) (Life Technologies, The Netherlands) was added and incubated at room temperature for 30 min. After washing, binding was visualized by conversion of 100 μL of aqueous tetramethylbenzydine (TMB) solution (Sigma Aldrich, The Netherlands), which was quenched with an aqueous 1 N H2 SO4 solution (100 μL). ..

    Blocking Assay:

    Article Title: Relevance of differential immunogenicity of human and mouse recombinant desmoglein-3 for the induction of Acantholytic autoantibodies in mice
    Article Snippet: .. Blots were treated for 1 h with a blocking buffer (5% non-fat dry milk in 200 m m NaCl with 0·1% Tween-20), and then incubated for 1 h each with the following reagents in succession with washing between each step: 1 : 2000 diluted rabbit anti-hdsg3 antibody [ ], and 1 : 3000 diluted HRP-conjugated goat antirabbit IgG (Caltag Laboratories, San Francisco, CA, USA). ..

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    Thermo Fisher hrp conjugated goat antirabbit igg
    Reactivity of anti-hdsg3 Abs with cells expressing mdsg3. The 293 cells were transfected with pSR α neo vector containing full-length mdsg3 cDNA, and stable cell lines were established (293 mdsg3). Cells were analysed by flow cytometry for the expression of mdsg3 by staining with rabbit anti-hdsg3 antibody (1 : 100), followed by FITC- conjugated goat <t>antirabbit</t> <t>IgG</t> (1 : 100). Propidium iodide was used to distinguish live from dead cells. — , 293 cells; ···, 293 mdsg3 cells.
    Hrp Conjugated Goat Antirabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat antirabbit igg/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat antirabbit igg - by Bioz Stars, 2021-04
    97/100 stars
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    99
    Thermo Fisher goat anti rabbit igg horseradish peroxidase conjugate
    Immunological cross reactions between N. sumatrana venom toxins as analyzed by immunoblotting. Venom toxins (10 µg each of phospholipase A 2 , neurotoxin and cardiotoxin) was electrophoresed on a SDS-PAGE gel (15%, reducing condition), and electro-transferred to a <t>PVDF</t> membrane. This was followed by subsequent incubation with primary antibody (anti-PLA 2 <t>IgG,</t> anti-NTX IgG and anti-CTX IgG (dilution of 1: 500) and goat anti-rabbit IgG-HRP (dilution of 1∶1000). Substrate solution (Novex HRP Chromogenic Substrate (TMB), Invitrogen) was added for colorimetric development.
    Goat Anti Rabbit Igg Horseradish Peroxidase Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg horseradish peroxidase conjugate/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg horseradish peroxidase conjugate - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Reactivity of anti-hdsg3 Abs with cells expressing mdsg3. The 293 cells were transfected with pSR α neo vector containing full-length mdsg3 cDNA, and stable cell lines were established (293 mdsg3). Cells were analysed by flow cytometry for the expression of mdsg3 by staining with rabbit anti-hdsg3 antibody (1 : 100), followed by FITC- conjugated goat antirabbit IgG (1 : 100). Propidium iodide was used to distinguish live from dead cells. — , 293 cells; ···, 293 mdsg3 cells.

    Journal: Clinical and Experimental Immunology

    Article Title: Relevance of differential immunogenicity of human and mouse recombinant desmoglein-3 for the induction of Acantholytic autoantibodies in mice

    doi: 10.1046/j.1365-2249.2003.02135.x

    Figure Lengend Snippet: Reactivity of anti-hdsg3 Abs with cells expressing mdsg3. The 293 cells were transfected with pSR α neo vector containing full-length mdsg3 cDNA, and stable cell lines were established (293 mdsg3). Cells were analysed by flow cytometry for the expression of mdsg3 by staining with rabbit anti-hdsg3 antibody (1 : 100), followed by FITC- conjugated goat antirabbit IgG (1 : 100). Propidium iodide was used to distinguish live from dead cells. — , 293 cells; ···, 293 mdsg3 cells.

    Article Snippet: Blots were treated for 1 h with a blocking buffer (5% non-fat dry milk in 200 m m NaCl with 0·1% Tween-20), and then incubated for 1 h each with the following reagents in succession with washing between each step: 1 : 2000 diluted rabbit anti-hdsg3 antibody [ ], and 1 : 3000 diluted HRP-conjugated goat antirabbit IgG (Caltag Laboratories, San Francisco, CA, USA).

    Techniques: Expressing, Transfection, Plasmid Preparation, Stable Transfection, Flow Cytometry, Cytometry, Staining

    MUC16/MUC16C interacts with β-catenin A. Expression profile of MUC16 in different cell lines. The cell lysates were analyzed by Western blot with the self-made mouse anti-MUC16 (40 μg input) or mouse anti-β-actin (8 μg input) antibody respectively. B. Ectopically expressed MUC16C interacts with endogenous β-catenin. HeLa cells were transfected with pcDNA3.3-HA-MUC16C or the empty vector as a control. At 24 h post-transfection, cells were lysed and subjected to immunoprecipitation with mouse anti-HA antibody, followed by Western blot with mouse anti-HA and rabbit anti-β-catenin antibodies separately. C. MUC16 and β-catenin interact with each other in vivo. Lysates of SKBR-3 cells were subjected to immunoprecipitation with the control IgG, rabbit anti-β-catenin and mouse anti-MUC16 antibodies respectively. The precipitates were then detected with indicated antibodies. D. C-terminus of β-catenin is essential for its interaction with MUC16. For the domain mapping experiment, structures of deletion mutants of β-catenin are shown on the top of the panel. Functional domains of β-catenin are indicated above the schema; the remaining fragments of each deletion mutant are shown in the diagram. HEK293T cells were co-transfected with pcDNA3.3-HA-MUC16C and different MYC-β-catenin mutants or the empty vector as a control. At 24 h post-transfection, cells were lysed and subjected to immunoprecipitation with rabbit anti-MYC antibody, followed by Western blot with mouse anti-HA or anti-MYC antibody.

    Journal: Oncotarget

    Article Title: C-terminus of MUC16 activates Wnt signaling pathway through its interaction with β-catenin to promote tumorigenesis and metastasis

    doi: 10.18632/oncotarget.9191

    Figure Lengend Snippet: MUC16/MUC16C interacts with β-catenin A. Expression profile of MUC16 in different cell lines. The cell lysates were analyzed by Western blot with the self-made mouse anti-MUC16 (40 μg input) or mouse anti-β-actin (8 μg input) antibody respectively. B. Ectopically expressed MUC16C interacts with endogenous β-catenin. HeLa cells were transfected with pcDNA3.3-HA-MUC16C or the empty vector as a control. At 24 h post-transfection, cells were lysed and subjected to immunoprecipitation with mouse anti-HA antibody, followed by Western blot with mouse anti-HA and rabbit anti-β-catenin antibodies separately. C. MUC16 and β-catenin interact with each other in vivo. Lysates of SKBR-3 cells were subjected to immunoprecipitation with the control IgG, rabbit anti-β-catenin and mouse anti-MUC16 antibodies respectively. The precipitates were then detected with indicated antibodies. D. C-terminus of β-catenin is essential for its interaction with MUC16. For the domain mapping experiment, structures of deletion mutants of β-catenin are shown on the top of the panel. Functional domains of β-catenin are indicated above the schema; the remaining fragments of each deletion mutant are shown in the diagram. HEK293T cells were co-transfected with pcDNA3.3-HA-MUC16C and different MYC-β-catenin mutants or the empty vector as a control. At 24 h post-transfection, cells were lysed and subjected to immunoprecipitation with rabbit anti-MYC antibody, followed by Western blot with mouse anti-HA or anti-MYC antibody.

    Article Snippet: Mouse anti-β-actin antibody was purchased from Santa Cruz Biotechnology, Inc. Goat-anti-mouse and goat anti-rabbit immunoglobulin G (IgG) horseradish peroxidase conjugates were purchased from Pierce Biotechnology, Inc.

    Techniques: Expressing, Western Blot, Transfection, Plasmid Preparation, Immunoprecipitation, In Vivo, Functional Assay, Mutagenesis

    Immunological cross reactions between N. sumatrana venom toxins as analyzed by immunoblotting. Venom toxins (10 µg each of phospholipase A 2 , neurotoxin and cardiotoxin) was electrophoresed on a SDS-PAGE gel (15%, reducing condition), and electro-transferred to a PVDF membrane. This was followed by subsequent incubation with primary antibody (anti-PLA 2 IgG, anti-NTX IgG and anti-CTX IgG (dilution of 1: 500) and goat anti-rabbit IgG-HRP (dilution of 1∶1000). Substrate solution (Novex HRP Chromogenic Substrate (TMB), Invitrogen) was added for colorimetric development.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Pharmacokinetics of Naja sumatrana (Equatorial Spitting Cobra) Venom and Its Major Toxins in Experimentally Envenomed Rabbits

    doi: 10.1371/journal.pntd.0002890

    Figure Lengend Snippet: Immunological cross reactions between N. sumatrana venom toxins as analyzed by immunoblotting. Venom toxins (10 µg each of phospholipase A 2 , neurotoxin and cardiotoxin) was electrophoresed on a SDS-PAGE gel (15%, reducing condition), and electro-transferred to a PVDF membrane. This was followed by subsequent incubation with primary antibody (anti-PLA 2 IgG, anti-NTX IgG and anti-CTX IgG (dilution of 1: 500) and goat anti-rabbit IgG-HRP (dilution of 1∶1000). Substrate solution (Novex HRP Chromogenic Substrate (TMB), Invitrogen) was added for colorimetric development.

    Article Snippet: Anti-PLA2 IgG, anti-NTX IgG or anti-CTX IgG (dilution of 1∶500 in TBS-Tween) was added to the PVDF membrane followed by incubation with Goat anti-rabbit IgG horseradish peroxidase conjugate (dilutions of 1∶1000) for 1 h. The chromogenic detection of the protein bands on the PVDF membrane was carried out by addition of the substrate solution (Novex HRP Chromogenic Substrate (TMB), Invitrogen).

    Techniques: SDS Page, Incubation, Proximity Ligation Assay

    Histological analysis of mice pre-treated with CPMO1v. BALB/c mice (6-day old, n = 6) were i.p injected with 15 μg/g CPMO1v or sCPMO1v. CHIKV infection and treatment regimen is carried out as aforementioned in Fig. 5 . All mice were sacrificed at day 7 p.i. and their hind limbs and liver were harvested, fixed with formalin, ethanol-dehydrated and paraffin embedded in sections for ( a–h ) H E staining or ( i–p ) IHC staining using primary rabbit anti-CHIKV E2 IgG followed by secondary goat anti-rabbit HRP IgG. ( d ) ( f ) Arrow indicates smudgy nuclei inclusions while arrowhead indicates multi-nucleated inclusions of pale necrotic hepatocytes. ( k , l ) Arrow indicates positive CHIKV antigen staining. Images were viewed and captured at 400× under Olympus microscope. Representative images at 50 μm scale are shown.

    Journal: Scientific Reports

    Article Title: Antiviral Phosphorodiamidate Morpholino Oligomers are Protective against Chikungunya Virus Infection on Cell-based and Murine Models

    doi: 10.1038/srep12727

    Figure Lengend Snippet: Histological analysis of mice pre-treated with CPMO1v. BALB/c mice (6-day old, n = 6) were i.p injected with 15 μg/g CPMO1v or sCPMO1v. CHIKV infection and treatment regimen is carried out as aforementioned in Fig. 5 . All mice were sacrificed at day 7 p.i. and their hind limbs and liver were harvested, fixed with formalin, ethanol-dehydrated and paraffin embedded in sections for ( a–h ) H E staining or ( i–p ) IHC staining using primary rabbit anti-CHIKV E2 IgG followed by secondary goat anti-rabbit HRP IgG. ( d ) ( f ) Arrow indicates smudgy nuclei inclusions while arrowhead indicates multi-nucleated inclusions of pale necrotic hepatocytes. ( k , l ) Arrow indicates positive CHIKV antigen staining. Images were viewed and captured at 400× under Olympus microscope. Representative images at 50 μm scale are shown.

    Article Snippet: For IHC, tissue samples were labeled with primary rabbit E2 antibody diluted to 1:100, followed by a secondary goat anti-rabbit HRP conjugate (Thermoscientific).

    Techniques: Mouse Assay, Injection, Infection, Staining, Immunohistochemistry, Microscopy