goat anti rabbit cy3  (Jackson Immuno)

 
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    Name:
    Cy 3 AffiniPure Goat Anti Rabbit IgG Fc fragment specific
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    111-165-008
    Price:
    153.0
    Category:
    Whole IgG Affinity Purified Antibodies
    Conjugate:
    Cyanine Cy 3
    Size:
    2 0 mg
    Format:
    Whole IgG
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno goat anti rabbit cy3
    A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. The PC-3 prostate cancer and NCM460 normal colon epithelial cells, with or without 1 h of preincubation in 10 mM NAC, were treated by 10 μM ATL or vehicle control for the indicated times. a , b Measurement of ROS in PC-3 cells by flow cytometry. Data from three independent experiments were presented as mean ± SD. c , d Measurement of ROS in NCM460 cells by flow cytometry. e , f Immunofluorescent staining of cellular 8-oxoG by <t>Cy3-conjugated</t> avidin. PC-3 cells were treated by 10 μM ATL for 12 h (scale bar: 10 μm). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. g Representative images of alkaline comet assay. PC-3 cells were treated by vehicle control or 10 μM ATL for 12 h. h The tail moment was defined as percentage of tail DNA × tail length, quantified using the TriTek CometScore software. i Immunofluorescence staining for PAR foci in PC-3 cells treated by 10 μM ATL for 12 h. DNA was counterstained with DAPI (scale bar: 5 μm). j Western blot analysis of PAR in PC-3 cells. Ten micrometer ATL resulted in a time-dependent increase in PAR levels which was blocked by NAC. n.s. not significant, ** p
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with the Fc portion of rabbit IgG heavy chain but not with the Fab portion of rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/goat anti rabbit cy3/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit cy3 - by Bioz Stars, 2021-07
    93/100 stars

    Images

    1) Product Images from "Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells"

    Article Title: Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells

    Journal: Oncogene

    doi: 10.1038/s41388-020-1191-x

    A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. The PC-3 prostate cancer and NCM460 normal colon epithelial cells, with or without 1 h of preincubation in 10 mM NAC, were treated by 10 μM ATL or vehicle control for the indicated times. a , b Measurement of ROS in PC-3 cells by flow cytometry. Data from three independent experiments were presented as mean ± SD. c , d Measurement of ROS in NCM460 cells by flow cytometry. e , f Immunofluorescent staining of cellular 8-oxoG by Cy3-conjugated avidin. PC-3 cells were treated by 10 μM ATL for 12 h (scale bar: 10 μm). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. g Representative images of alkaline comet assay. PC-3 cells were treated by vehicle control or 10 μM ATL for 12 h. h The tail moment was defined as percentage of tail DNA × tail length, quantified using the TriTek CometScore software. i Immunofluorescence staining for PAR foci in PC-3 cells treated by 10 μM ATL for 12 h. DNA was counterstained with DAPI (scale bar: 5 μm). j Western blot analysis of PAR in PC-3 cells. Ten micrometer ATL resulted in a time-dependent increase in PAR levels which was blocked by NAC. n.s. not significant, ** p
    Figure Legend Snippet: A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. The PC-3 prostate cancer and NCM460 normal colon epithelial cells, with or without 1 h of preincubation in 10 mM NAC, were treated by 10 μM ATL or vehicle control for the indicated times. a , b Measurement of ROS in PC-3 cells by flow cytometry. Data from three independent experiments were presented as mean ± SD. c , d Measurement of ROS in NCM460 cells by flow cytometry. e , f Immunofluorescent staining of cellular 8-oxoG by Cy3-conjugated avidin. PC-3 cells were treated by 10 μM ATL for 12 h (scale bar: 10 μm). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. g Representative images of alkaline comet assay. PC-3 cells were treated by vehicle control or 10 μM ATL for 12 h. h The tail moment was defined as percentage of tail DNA × tail length, quantified using the TriTek CometScore software. i Immunofluorescence staining for PAR foci in PC-3 cells treated by 10 μM ATL for 12 h. DNA was counterstained with DAPI (scale bar: 5 μm). j Western blot analysis of PAR in PC-3 cells. Ten micrometer ATL resulted in a time-dependent increase in PAR levels which was blocked by NAC. n.s. not significant, ** p

    Techniques Used: Flow Cytometry, Staining, Avidin-Biotin Assay, Software, Alkaline Single Cell Gel Electrophoresis, Immunofluorescence, Western Blot

    2) Product Images from "IRF-1 Promotes Inflammation Early after Ischemic Acute Kidney Injury"

    Article Title: IRF-1 Promotes Inflammation Early after Ischemic Acute Kidney Injury

    Journal: Journal of the American Society of Nephrology : JASN

    doi: 10.1681/ASN.2008080843

    IRF-1 immunohistology of wild-type ischemic kidneys. (A) Ischemic kidney. This shows a black and white photomicrograph of an ischemic kidney at 4 h of reperfusion. The kidney has been stained with rabbit anti–IRF-1 followed by Cy3 goat anti-rabbit
    Figure Legend Snippet: IRF-1 immunohistology of wild-type ischemic kidneys. (A) Ischemic kidney. This shows a black and white photomicrograph of an ischemic kidney at 4 h of reperfusion. The kidney has been stained with rabbit anti–IRF-1 followed by Cy3 goat anti-rabbit

    Techniques Used: Staining

    Related Articles

    Incubation:

    Article Title: Tomato Ringspot Virus Proteins Containing the Nucleoside Triphosphate Binding Domain Are Transmembrane Proteins That Associate with the Endoplasmic Reticulum and Cofractionate with Replication Complexes †
    Article Snippet: Subsequently, the protoplasts were incubated for 1 h with the anti-NTB antibodies (diluted 1:5,000 in blocking solution). .. After five washes with PBS, the protoplasts were incubated with goat anti-rabbit antibodies conjugated to Cy3 (Jackson Immuno Research Laboratories), diluted 1:250 in blocking buffer, for 1 h. After five washes with PBS, the coverslips were mounted on microscope slides by using the ProLong Antifade kit (Molecular Probes, Inc.). .. Protoplasts were viewed by fluorescence microscopy with an Axiophot microscope (Zeiss).

    Article Title: IRF-1 Promotes Inflammation Early after Ischemic Acute Kidney Injury
    Article Snippet: Diluted primary antibody, rabbit anti-mouse IRF-1 at 1:100 (cat. no. sc-640; Santa Cruz Biotechnology, Santa Cruz, CA) and normal rabbit IgG (cat. no. sc-2027; Santa Cruz Biotechnology) at 1:100 were applied to the samples, and the sections were incubated overnight at 4°C. .. Unbound primary antibody was removed from slides in TBST wash, and the slides were incubated with secondary antibody, goat anti-rabbit-Cy3 at 1:500 (cat. no. 111165144; Jackson Immunoresearch Laboratories, West Grove, PA) at room temperature in the dark for 1 h and rinsed with TBST. .. The samples were then incubated with fluorescein-labeled Lotus tetragonolobus lectin (cat. no. FL-1321; Vector Laboratories) for 2 h in the dark.

    Article Title: Schistosome Infection Stimulates Host CD4+ T Helper Cell and B-Cell Responses against a Novel Egg Antigen, Thioredoxin Peroxidase
    Article Snippet: .. The slides were incubated for 1 h at room temperature with 1:500 goat anti-rabbit Cy3-conjugated antibody (Jackson ImmunoResearch). ..

    Blocking Assay:

    Article Title: Tomato Ringspot Virus Proteins Containing the Nucleoside Triphosphate Binding Domain Are Transmembrane Proteins That Associate with the Endoplasmic Reticulum and Cofractionate with Replication Complexes †
    Article Snippet: Subsequently, the protoplasts were incubated for 1 h with the anti-NTB antibodies (diluted 1:5,000 in blocking solution). .. After five washes with PBS, the protoplasts were incubated with goat anti-rabbit antibodies conjugated to Cy3 (Jackson Immuno Research Laboratories), diluted 1:250 in blocking buffer, for 1 h. After five washes with PBS, the coverslips were mounted on microscope slides by using the ProLong Antifade kit (Molecular Probes, Inc.). .. Protoplasts were viewed by fluorescence microscopy with an Axiophot microscope (Zeiss).

    Microscopy:

    Article Title: Tomato Ringspot Virus Proteins Containing the Nucleoside Triphosphate Binding Domain Are Transmembrane Proteins That Associate with the Endoplasmic Reticulum and Cofractionate with Replication Complexes †
    Article Snippet: Subsequently, the protoplasts were incubated for 1 h with the anti-NTB antibodies (diluted 1:5,000 in blocking solution). .. After five washes with PBS, the protoplasts were incubated with goat anti-rabbit antibodies conjugated to Cy3 (Jackson Immuno Research Laboratories), diluted 1:250 in blocking buffer, for 1 h. After five washes with PBS, the coverslips were mounted on microscope slides by using the ProLong Antifade kit (Molecular Probes, Inc.). .. Protoplasts were viewed by fluorescence microscopy with an Axiophot microscope (Zeiss).

    Staining:

    Article Title: MeCP2: a novel Huntingtin interactor
    Article Snippet: Antibody staining was similar to that discussed earlier with the elimination of RNase A treatment and Sytox Orange staining. .. The second protein stained in these reactions was counterstained with goat anti-mouse or goat anti-rabbit antibody conjugated to Cy3 (1:200 dilution; Jackson ImmunoResearch, West Grove, PA, USA). .. Cells were coverslipped in GVA Mount and imaged on a conventional epifluorescence microscope prior to FRET–FLIM imaging to ensure proper staining.

    Immunostaining:

    Article Title: The Drosophila gene Start1: A putative cholesterol transporter and key regulator of ecdysteroid synthesis
    Article Snippet: .. Whole-mount immunostaining was done as described ( ) by using goat anti-rabbit Cy3-labeled secondary antibody (Jackson ImmunoResearch). .. Preparations were examined with a Zeiss Axiophot fluorescence microscope and a Quantix (Photometrics, Tucson, AZ) video camera.

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  • 93
    Jackson Immuno goat anti rabbit cy3
    A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. The PC-3 prostate cancer and NCM460 normal colon epithelial cells, with or without 1 h of preincubation in 10 mM NAC, were treated by 10 μM ATL or vehicle control for the indicated times. a , b Measurement of ROS in PC-3 cells by flow cytometry. Data from three independent experiments were presented as mean ± SD. c , d Measurement of ROS in NCM460 cells by flow cytometry. e , f Immunofluorescent staining of cellular 8-oxoG by <t>Cy3-conjugated</t> avidin. PC-3 cells were treated by 10 μM ATL for 12 h (scale bar: 10 μm). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. g Representative images of alkaline comet assay. PC-3 cells were treated by vehicle control or 10 μM ATL for 12 h. h The tail moment was defined as percentage of tail DNA × tail length, quantified using the TriTek CometScore software. i Immunofluorescence staining for PAR foci in PC-3 cells treated by 10 μM ATL for 12 h. DNA was counterstained with DAPI (scale bar: 5 μm). j Western blot analysis of PAR in PC-3 cells. Ten micrometer ATL resulted in a time-dependent increase in PAR levels which was blocked by NAC. n.s. not significant, ** p
    Goat Anti Rabbit Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit cy3/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit cy3 - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

    86
    Jackson Immuno cy3 conjugated goat anti rabbit igg
    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit <t>IgG</t> (green) and mono-clonal rat antibody against mouse CD68 followed by <t>Cy3-con-jugated</t> anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.
    Cy3 Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated goat anti rabbit igg/product/Jackson Immuno
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated goat anti rabbit igg - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    94
    Jackson Immuno cy3 goat anti rabbit ab
    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat <t>Cy3</t> <t>anti-rabbit</t> Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).
    Cy3 Goat Anti Rabbit Ab, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 goat anti rabbit ab/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 goat anti rabbit ab - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. The PC-3 prostate cancer and NCM460 normal colon epithelial cells, with or without 1 h of preincubation in 10 mM NAC, were treated by 10 μM ATL or vehicle control for the indicated times. a , b Measurement of ROS in PC-3 cells by flow cytometry. Data from three independent experiments were presented as mean ± SD. c , d Measurement of ROS in NCM460 cells by flow cytometry. e , f Immunofluorescent staining of cellular 8-oxoG by Cy3-conjugated avidin. PC-3 cells were treated by 10 μM ATL for 12 h (scale bar: 10 μm). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. g Representative images of alkaline comet assay. PC-3 cells were treated by vehicle control or 10 μM ATL for 12 h. h The tail moment was defined as percentage of tail DNA × tail length, quantified using the TriTek CometScore software. i Immunofluorescence staining for PAR foci in PC-3 cells treated by 10 μM ATL for 12 h. DNA was counterstained with DAPI (scale bar: 5 μm). j Western blot analysis of PAR in PC-3 cells. Ten micrometer ATL resulted in a time-dependent increase in PAR levels which was blocked by NAC. n.s. not significant, ** p

    Journal: Oncogene

    Article Title: Synergistic lethality between PARP-trapping and alantolactone-induced oxidative DNA damage in homologous recombination-proficient cancer cells

    doi: 10.1038/s41388-020-1191-x

    Figure Lengend Snippet: A nontoxic dose of ATL induces oxidative DNA damage and activates PARP in cancer cells. The PC-3 prostate cancer and NCM460 normal colon epithelial cells, with or without 1 h of preincubation in 10 mM NAC, were treated by 10 μM ATL or vehicle control for the indicated times. a , b Measurement of ROS in PC-3 cells by flow cytometry. Data from three independent experiments were presented as mean ± SD. c , d Measurement of ROS in NCM460 cells by flow cytometry. e , f Immunofluorescent staining of cellular 8-oxoG by Cy3-conjugated avidin. PC-3 cells were treated by 10 μM ATL for 12 h (scale bar: 10 μm). Nuclear 8-oxoG intensity was measured using the ImageJ software and the data were processed by the Prism software. g Representative images of alkaline comet assay. PC-3 cells were treated by vehicle control or 10 μM ATL for 12 h. h The tail moment was defined as percentage of tail DNA × tail length, quantified using the TriTek CometScore software. i Immunofluorescence staining for PAR foci in PC-3 cells treated by 10 μM ATL for 12 h. DNA was counterstained with DAPI (scale bar: 5 μm). j Western blot analysis of PAR in PC-3 cells. Ten micrometer ATL resulted in a time-dependent increase in PAR levels which was blocked by NAC. n.s. not significant, ** p

    Article Snippet: Secondary antibodies include goat anti-mouse-Alexa 488, goat anti-rabbit-Cy3, goat anti-rat-Alexa-488, goat anti-mouse-horseradish peroxidase and goat anti-rabbit-horseradish peroxidase (Jackson ImmunoResearch, West Grove, PA, USA).

    Techniques: Flow Cytometry, Staining, Avidin-Biotin Assay, Software, Alkaline Single Cell Gel Electrophoresis, Immunofluorescence, Western Blot

    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: TLR4 mediates LPS-induced HO-1 expression in mouse liver: Role of TNF-α and IL-1β

    doi: 10.3748/wjg.v9.i8.1799

    Figure Lengend Snippet: Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Article Snippet: Rat IgG and Cy3-conjugated goat anti-rabbit IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).

    Techniques: Staining

    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Journal: The Journal of Cell Biology

    Article Title: Regulated Targeting of BAX to Mitochondria

    doi:

    Figure Lengend Snippet: Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Article Snippet: Anti-mBAX (P-19) Ab ( Santa Cruz Biotechnology , Santa Cruz, CA) and Cy3 goat anti–rabbit Ab (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to detect BAX immunoreactivity.

    Techniques: Confocal Microscopy, Labeling, Fluorescence, Staining

    Wnt-1, β-catenin and E-cadherin proteins localize predominantly in the hepatocytes of an adult rat liver. (A) Western blot demonstrates wnt-1 in hepatocytes of a resting adult liver. (B) β-Catenin is seen in hepatocytes and Kupffer cells. (C) Immunofluorescence staining reveals localizing of β-catenin ( green ) on the membranes and cytoplasm (immediately next to the membrane) in the rat liver uniformly. Some hepatocytes show nuclear localization ( arrowheads ). Inset reveals nuclear localization as depicted by the yellow color by overlay ( arrowhead ) of Cy3 ( red ) for β-catenin and Sytox Green for the nuclei. Bar = 10 μm. (D) E-cadherin ( red ) localizes to the membrane ( arrowheads ) and the cytoplasm (immediate proximity to the membrane) of the hepatocytes in a normal rat liver. Bar = 10 μm.

    Journal: Hepatology (Baltimore, Md.)

    Article Title: Changes in Wnt/?-Catenin Pathway During Regulated Growth in Rat Liver Regeneration

    doi: 10.1053/jhep.2001.23786

    Figure Lengend Snippet: Wnt-1, β-catenin and E-cadherin proteins localize predominantly in the hepatocytes of an adult rat liver. (A) Western blot demonstrates wnt-1 in hepatocytes of a resting adult liver. (B) β-Catenin is seen in hepatocytes and Kupffer cells. (C) Immunofluorescence staining reveals localizing of β-catenin ( green ) on the membranes and cytoplasm (immediately next to the membrane) in the rat liver uniformly. Some hepatocytes show nuclear localization ( arrowheads ). Inset reveals nuclear localization as depicted by the yellow color by overlay ( arrowhead ) of Cy3 ( red ) for β-catenin and Sytox Green for the nuclei. Bar = 10 μm. (D) E-cadherin ( red ) localizes to the membrane ( arrowheads ) and the cytoplasm (immediate proximity to the membrane) of the hepatocytes in a normal rat liver. Bar = 10 μm.

    Article Snippet: Secondary antibodies used for this study were goat anti-rabbit Cy3, goat anti-mouse Cy3 (Jackson ImmunoResearch Laboratories, West Grove, PA), or Alexa 488 (Molecular Probes, Eugene, OR) at a 1:3,000 (for Cy3) or 1:500 (for Alexa 488) dilution.

    Techniques: Western Blot, Immunofluorescence, Staining