goat anti mouse igg2b alexa fluor 647 conjugate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher goat anti mouse igg2b alexa fluor 647 conjugate
    Goat Anti Mouse Igg2b Alexa Fluor 647 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg2b alexa fluor 647 conjugate/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg2b alexa fluor 647 conjugate - by Bioz Stars, 2020-09
    92/100 stars

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    Related Articles

    Marker:

    Article Title: Human T-Lymphotropic Virus Type 1 Mitochondrion-Localizing Protein p13II Sensitizes Jurkat T Cells to Ras-Mediated Apoptosis
    Article Snippet: .. After three washes, cells were incubated with mouse anti-human cytochrome c oxidase complex IV immunoglobulin G2a (IgG2a; 1:100) (Molecular Probes) as a mitochondrion-specific marker and with a secondary goat anti-mouse IgG2a Alexa Fluor 647 conjugate (1:200) (Molecular Probes) for 1 h each at room temperature. .. The p13II protein was stained with mouse anti-HA IgG1 (clone 16B-12; Covance Research Products) after preincubation with anti-mouse IgG1-Fc F(ab)2 Zenon anti-HA (Molecular Probe) for 1 h at room temperature.

    Incubation:

    Article Title: Human T-Lymphotropic Virus Type 1 Mitochondrion-Localizing Protein p13II Sensitizes Jurkat T Cells to Ras-Mediated Apoptosis
    Article Snippet: .. After three washes, cells were incubated with mouse anti-human cytochrome c oxidase complex IV immunoglobulin G2a (IgG2a; 1:100) (Molecular Probes) as a mitochondrion-specific marker and with a secondary goat anti-mouse IgG2a Alexa Fluor 647 conjugate (1:200) (Molecular Probes) for 1 h each at room temperature. .. The p13II protein was stained with mouse anti-HA IgG1 (clone 16B-12; Covance Research Products) after preincubation with anti-mouse IgG1-Fc F(ab)2 Zenon anti-HA (Molecular Probe) for 1 h at room temperature.

    Article Title: Competition between antagonistic complement factors for a single protein on N. meningitidis rules disease susceptibility
    Article Snippet: .. Cells were washed twice in 0.05% BSA, then resuspended in 50 μl of goat anti-mouse IgG-Alexa Fluor 647 conjugate (1 in 1000 dilution in PBS; Molecular Probes, Life Technologies) and incubated for 30 min at 4°C. .. Samples were run on a FACSCalibur (BD Biosciences), and at least 104 events recorded before results were analysed by calculating the geometric mean FL-4 in FlowJo vX software (Tree Star).

    Binding Assay:

    Article Title: Competition between antagonistic complement factors for a single protein on N. meningitidis rules disease susceptibility
    Article Snippet: .. CFHR3 binding was detected with the anti-CFHR3 mAb (HSL1 this study) or an anti-human CFH mAb (MRC OX24, 0.1 μg/ml) , followed by incubating with goat anti-mouse IgG-Alexa Fluor 647 conjugate (1 in 1000 dilution in PBS; Molecular Probes, Life Technologies) and analysed by flow cytometry. .. At least 104 events were recorded before results were analysed by calculating the geometric mean FL-4 in FlowJo vX software (Tree Star).

    Flow Cytometry:

    Article Title: Competition between antagonistic complement factors for a single protein on N. meningitidis rules disease susceptibility
    Article Snippet: .. CFHR3 binding was detected with the anti-CFHR3 mAb (HSL1 this study) or an anti-human CFH mAb (MRC OX24, 0.1 μg/ml) , followed by incubating with goat anti-mouse IgG-Alexa Fluor 647 conjugate (1 in 1000 dilution in PBS; Molecular Probes, Life Technologies) and analysed by flow cytometry. .. At least 104 events were recorded before results were analysed by calculating the geometric mean FL-4 in FlowJo vX software (Tree Star).

    Cytometry:

    Article Title: Competition between antagonistic complement factors for a single protein on N. meningitidis rules disease susceptibility
    Article Snippet: .. CFHR3 binding was detected with the anti-CFHR3 mAb (HSL1 this study) or an anti-human CFH mAb (MRC OX24, 0.1 μg/ml) , followed by incubating with goat anti-mouse IgG-Alexa Fluor 647 conjugate (1 in 1000 dilution in PBS; Molecular Probes, Life Technologies) and analysed by flow cytometry. .. At least 104 events were recorded before results were analysed by calculating the geometric mean FL-4 in FlowJo vX software (Tree Star).

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    Thermo Fisher goat anti mouse igg h l cross adsorbed secondary antibody
    Goat Anti Mouse Igg H L Cross Adsorbed Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l cross adsorbed secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l cross adsorbed secondary antibody - by Bioz Stars, 2020-09
    99/100 stars
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    92
    Thermo Fisher alexa fluor 594 phalloidin
    Immunofluorescence analyses of focal adhesions and actin filaments in WT and stable B16-F10 cells. In both groups (A B), cells were settled onto fibronectin-coated coverslip-bottom culture dish and incubated for 2 h under culture conditions. Cells were fixed and permeabilized followed by antibody staining for the focal adhesion protein paxillin. Actin filaments were stained with <t>Alexa</t> Fluor® <t>594-phalloidin</t> and nuclei were stained with DAPI. (A) Polarized cells. (B) Non-polarized cells. GFP and GFP-kindlin-3 were detected in the B16-F10 stable cells. Consistent with previous findings in HUVECs , kindlin-3 did not show punctate localization at mature focal adhesion sites containing paxillin. Scale bar represents 10 μm. Representative images of cells from 2 independent experiments are shown. Images were processed using the auto-min/max function in the ZEN2012 software for optimal setting for contrast and brightness. Also see Supporting Information Fig. S10.
    Alexa Fluor 594 Phalloidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 594 phalloidin/product/Thermo Fisher
    Average 92 stars, based on 317 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 phalloidin - by Bioz Stars, 2020-09
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    91
    Thermo Fisher alexa fluor 647 conjugated goat anti mouse igg
    Flow cytometric quantification of induced GalNAc-T2. A , cells were induced for 48 h with doxycycline ( Dox ) before being fixed, permeabilized, and stained with anti-GalNAc-T2 primary antibody and <t>Alexa</t> <t>Fluor</t> 647 secondary antibody. B , enlargement of HEK ind T2 induced from 0 to 16 ng/ml doxycycline with a help line centered at the peak of 0 ng/ml. The induction is biphasic with a distinct global right shift of the lower-expressing population. Data are representative of two biological and two technical replicates.
    Alexa Fluor 647 Conjugated Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated goat anti mouse igg/product/Thermo Fisher
    Average 91 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated goat anti mouse igg - by Bioz Stars, 2020-09
    91/100 stars
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    99
    Thermo Fisher alexa fluor 647 conjugated donkey anti mouse secondary
    Specific binding of aptamer A5 to GLUT1 in human tissue. (A) Human brain cortical tissue was labeled with a GLUT1 <t>Alexa</t> Fluor 488-conjugated antibody, FAM-labeled aptamer A5, or FAM-labeled scrambled aptamer. (B) Human tissue was co-labeled with lectin and the GLUT1 antibody. (C) Human tissue was co-labeled with lectin and the Alexa Fluor 647-labeled aptamer A5. All immunolabeling was performed with two separate tissue samples, with three images taken from each tissue section to validate expression patterns. The representative images are prov ided with DAPI co-labeling (blue) (scale bar: 200 μm).
    Alexa Fluor 647 Conjugated Donkey Anti Mouse Secondary, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated donkey anti mouse secondary/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated donkey anti mouse secondary - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Immunofluorescence analyses of focal adhesions and actin filaments in WT and stable B16-F10 cells. In both groups (A B), cells were settled onto fibronectin-coated coverslip-bottom culture dish and incubated for 2 h under culture conditions. Cells were fixed and permeabilized followed by antibody staining for the focal adhesion protein paxillin. Actin filaments were stained with Alexa Fluor® 594-phalloidin and nuclei were stained with DAPI. (A) Polarized cells. (B) Non-polarized cells. GFP and GFP-kindlin-3 were detected in the B16-F10 stable cells. Consistent with previous findings in HUVECs , kindlin-3 did not show punctate localization at mature focal adhesion sites containing paxillin. Scale bar represents 10 μm. Representative images of cells from 2 independent experiments are shown. Images were processed using the auto-min/max function in the ZEN2012 software for optimal setting for contrast and brightness. Also see Supporting Information Fig. S10.

    Journal: Cell Adhesion & Migration

    Article Title: Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis

    doi: 10.1080/19336918.2016.1243645

    Figure Lengend Snippet: Immunofluorescence analyses of focal adhesions and actin filaments in WT and stable B16-F10 cells. In both groups (A B), cells were settled onto fibronectin-coated coverslip-bottom culture dish and incubated for 2 h under culture conditions. Cells were fixed and permeabilized followed by antibody staining for the focal adhesion protein paxillin. Actin filaments were stained with Alexa Fluor® 594-phalloidin and nuclei were stained with DAPI. (A) Polarized cells. (B) Non-polarized cells. GFP and GFP-kindlin-3 were detected in the B16-F10 stable cells. Consistent with previous findings in HUVECs , kindlin-3 did not show punctate localization at mature focal adhesion sites containing paxillin. Scale bar represents 10 μm. Representative images of cells from 2 independent experiments are shown. Images were processed using the auto-min/max function in the ZEN2012 software for optimal setting for contrast and brightness. Also see Supporting Information Fig. S10.

    Article Snippet: Cells were washed 3 times in PBS followed by incubation in PBS containing Alexa Fluor® 594-phalloidin (0.27 ng/mL) (Thermo Fisher scientific, Waltham, MA, cat. no. A12381) Alexa Fluor® 647-conjugated goat anti-mouse IgG (H+ L) (1:400 dilution) (Thermo Fisher scientific, cat. no. ), and DAPI (0.1 µg/mL) for 3 h at RT.

    Techniques: Immunofluorescence, Incubation, Staining, Software

    Flow cytometric quantification of induced GalNAc-T2. A , cells were induced for 48 h with doxycycline ( Dox ) before being fixed, permeabilized, and stained with anti-GalNAc-T2 primary antibody and Alexa Fluor 647 secondary antibody. B , enlargement of HEK ind T2 induced from 0 to 16 ng/ml doxycycline with a help line centered at the peak of 0 ng/ml. The induction is biphasic with a distinct global right shift of the lower-expressing population. Data are representative of two biological and two technical replicates.

    Journal: The Journal of Biological Chemistry

    Article Title: Probing the contribution of individual polypeptide GalNAc-transferase isoforms to the O-glycoproteome by inducible expression in isogenic cell lines

    doi: 10.1074/jbc.RA118.004516

    Figure Lengend Snippet: Flow cytometric quantification of induced GalNAc-T2. A , cells were induced for 48 h with doxycycline ( Dox ) before being fixed, permeabilized, and stained with anti-GalNAc-T2 primary antibody and Alexa Fluor 647 secondary antibody. B , enlargement of HEK ind T2 induced from 0 to 16 ng/ml doxycycline with a help line centered at the peak of 0 ng/ml. The induction is biphasic with a distinct global right shift of the lower-expressing population. Data are representative of two biological and two technical replicates.

    Article Snippet: After washing in PBS, cells were incubated with Alexa Fluor 647–conjugated goat anti-mouse IgG (A-21235, Thermo Scientific) (1:1000 in PBS, 1% BSA) for 1 h at RT, washed, and stored in PBS, 1% BSA at 4 °C until analysis.

    Techniques: Flow Cytometry, Staining, Expressing

    Specific binding of aptamer A5 to GLUT1 in human tissue. (A) Human brain cortical tissue was labeled with a GLUT1 Alexa Fluor 488-conjugated antibody, FAM-labeled aptamer A5, or FAM-labeled scrambled aptamer. (B) Human tissue was co-labeled with lectin and the GLUT1 antibody. (C) Human tissue was co-labeled with lectin and the Alexa Fluor 647-labeled aptamer A5. All immunolabeling was performed with two separate tissue samples, with three images taken from each tissue section to validate expression patterns. The representative images are prov ided with DAPI co-labeling (blue) (scale bar: 200 μm).

    Journal: bioRxiv

    Article Title: CRISPR-mediated isogenic cell-SELEX approach for generating highly specific aptamers against native membrane proteins

    doi: 10.1101/2020.02.17.949768

    Figure Lengend Snippet: Specific binding of aptamer A5 to GLUT1 in human tissue. (A) Human brain cortical tissue was labeled with a GLUT1 Alexa Fluor 488-conjugated antibody, FAM-labeled aptamer A5, or FAM-labeled scrambled aptamer. (B) Human tissue was co-labeled with lectin and the GLUT1 antibody. (C) Human tissue was co-labeled with lectin and the Alexa Fluor 647-labeled aptamer A5. All immunolabeling was performed with two separate tissue samples, with three images taken from each tissue section to validate expression patterns. The representative images are prov ided with DAPI co-labeling (blue) (scale bar: 200 μm).

    Article Snippet: Antibody-incubated tissue sections were followed by treatment with Alexa Fluor 647 conjugated donkey anti-mouse secondary (Thermo Fisher, A-31573) for 1 hour.

    Techniques: Binding Assay, Labeling, Immunolabeling, Expressing