Goat anti Mouse IgG
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1) Product Images from "Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)"
Article Title: Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
Journal: Nature Communications
Figure Legend Snippet: Pre-labeling Ex- d STORM. a Simulated intensity profiles using a cylindrical distribution function to describe unexpanded or 3.2x expanded immunostained microtubules (labeled with IgG antibodies or DNA modified IgG antibodies pre-expansion) and resulting peak-to-peak distances of the cross-sectional profiles. b d STORM image of expanded and re-embedded α- and β-tubulin pre-labeled with secondary Alexa Fluor 532 IgG antibodies (Al532) using the MA-NHS/GA method 6 , i.e. antibodies are cross-linked with glutaraldehyde (GA) into the hydrogel (Antibody-Al532 (GA)). c Zoom in of white boxed region in ( b ). d Averaged cross-sectional profile of 8 microtubule segments with a length between 1.5–6.4 µm and 28.6 µm in total measured in 4 expanded cells. e Histogram of peak-to-peak distance distribution with normalized normal curve (red) of microtubule segments analyzed in ( d ) at n = 8 microtubule segments in 4 cells from 1 expansion experiment with a mean distance of 133.8 ± 13.2 nm (mean ± sd). f Unexpanded d STORM image of ssDNA-Cy5 secondary antibody hybridized with Cy5 bearing oligonucleotides pre-expansion (DNA-Cy5 protocol). g Magnified view of white boxed region in ( f ). h Average cross-sectional profile of 7 microtubule segments with a length between 1.0–1.8 µm and 8.7 µm in total. i Histogram of peak-to-peak distances with normalized normal distribution curve (red) of the data analyzed in ( h ) along n = 7 microtubule segments in 2 cells from 1 experiment with a mean distance of 43.9 ± 3.7 nm (mean ± sd). j Expanded d STORM image of microtubules labeled with α-tubulin and dsDNA (DNA-Al532) conjugated secondary antibodies exhibiting a methacryloyl group to crosslink the DNA with fluorophores pre-expansion into the hydrogel (original ExM trifunctional label concept) 1 . k Zoom-in of white boxed region in ( j ). l Average intensity profile of 26 microtubule segments with a length of 2.4–10.7 µm and 118.6 µm in total. m Histogram of peak-to-peak distances with normalized normal distribution curve (red) determined from n = 26 microtubule segments in 4 cells from 1 expanded sample showing a mean distance of 226.7 ± 15.3 nm (mean ± sd). n d STORM image of α- and β-tubulin expanded according to the DNA-Cy5 protocol strategy with labels at Cy5-bearing oligonucleotides introduced post-re-embedding. o Zoom in of white boxed region in ( n ). p Average intensity profile of 15 microtubule segments with a length between 1.6–25.1 µm and a total length of 126.0 µm in 1 expanded sample. q Histogram of peak-to-peak distances with normalized normal distribution curve (red) determined by fitting the cross-sectional profiles analyzed in ( p ) along n = 22 microtubule segments in 4 cells from 1 expanded sample showing a mean distance of 201.0 ± 12.9 nm (mean ± sd). The small logos in the upper left corner symbolize the labeling method, e.g. pre- and post-immunolabeled with or without DNA-linker, respectively. Scale bars, 2 µm ( b , f , j , n ), 500 nm ( c , g , k , o ).
Techniques Used: Labeling, Modification, Immunolabeling
Figure Legend Snippet: Re-embedding enables Ex- d STORM. a Model of microtubules with an outer diameter of 25 nm stained with conventional primary (pab) and fluorescently labeled secondary IgG antibodies (sab) results in a total diameter of 60 nm with a linkage error (defined by the size of the primary and secondary antibody) of 17.5 nm 22 . b d STORM image of pre-labeled proExM expanded and re-embedded Cos-7 cells stained with primary antibodies against α-tubulin and secondary Alexa Fluor 532 conjugated antibodies (Al532). The small logo in the upper left corner symbolizes that microtubules have been immunolabeled before expansion (pre-labeled). c Zoom in on highlighted region in ( b ). d Averaged cross-sectional profile of nine microtubule segments with a total length of 29.1 µm (segment lengths range from 2.1-4.5 µm) measured in two cells from 1 expanded sample. e Histogram of peak-to-peak distances with normalized normal distribution curve (red) determined by bi-Gaussian fitting of the data analyzed in ( c ) with an average distance of 137.1 ± 10.1 nm (mean ± sd). The data were obtained from n = 9 microtubule segments in 2 cells from 1 expanded sample. f Unexpanded Cos-7 cells labeled with an anti α-tubulin primary antibody and Alexa Fluor 532 (Al532) conjugated IgG secondary antibodies. The small logo in the upper left corner symbolizes that microtubules have been immunolabeled and not expanded. g Zoom in of the white boxed region in ( f ). h Average intensity profile of 35 microtubule segments with a length between 1.1 and 5.8 µm (mean = 2.0 µm) and a total length of 69.6 µm analyzed in 12 d STORM images. i Histogram of peak-to-peak distances with normalized normal distribution curve (red) determined by bi-Gaussian fitting of cross-sectional profiles of the analyzed microtubule segments in ( h ) with a mean peak-to-peak distance of 36.2 ± 5.4 nm (mean ± sd). The data were obtained from n =35 microtubule segments in 12 cells and 3 independent experiments. Scale bars, 2 µm ( b , f ), 500 nm ( c , g ).
Techniques Used: Staining, Labeling, Immunolabeling
2) Product Images from "CD1c molecules broadly survey the endocytic system"
Article Title: CD1c molecules broadly survey the endocytic system
Journal: Proceedings of the National Academy of Sciences of the United States of America
Figure Legend Snippet: Colocalization of CD1c with ARF6-T27N. HeLa cell transfectants expressing CD1c were grown on coverslips and transiently supertransfected with ARF6-T27N cDNA. The cells were then fixed and permeabilized, and double-labeling with mouse anti-CD1c antibody (detected with Texas Red-conjugated donkey anti-mouse IgG) and rabbit anti-ARF6 antibody (detected with FITC-conjugated donkey anti-rabbit IgG) was performed. Fluorescent confocal images were obtained for CD1c ( A ) and ARF6 ( B ). The two images then were superimposed to detect vesicles expressing both CD1c and ARF6 ( C , yellow vesicles shown with arrowheads). (Scale bars = 5 μm.)
Techniques Used: Expressing, Labeling
Figure Legend Snippet: Colocalization of CD1a, -b, and -c with LAMP-1. Monocyte-derived dendritic cells were fixed and permeablized after a cytospin procedure. The cells were then double-labeled with mouse mAbs to CD1a ( A ), CD1b ( D ), or CD1c ( G ) (detected with Texas Red-conjugated donkey anti-mouse IgG) and a rabbit antiserum against human LAMP-1 ( B , E , and H ) (detected with FITC-conjugated donkey anti-rabbit IgG) and analyzed by confocal microscopy. The corresponding red and green fluorescent confocal images then were superimposed to detect any cellular compartments expressing both CD1 and LAMP-1 ( C , F , and I , yellow vesicles). (Scale bars = 5 μm.)
Techniques Used: Derivative Assay, Labeling, Confocal Microscopy, Expressing
Article Title: Anti-C1q autoantibodies deposit in glomeruli but are only pathogenic in combination with glomerular C1q-containing immune complexes
Article Snippet: .. Sections were stained for the presence of mouse IgG, rabbit IgG, and mouse C1q using
Article Title: Identification of the Mycobacterium ulcerans Protein MUL_3720 as a Promising Target for the Development of a Diagnostic Test for Buruli Ulcer
Article Snippet: Agarose blocks were embedded into paraffin, cut in 3 μm sections and transferred onto microscopy glass slides (Thermo Scientific). .. Bacteria were stained with mAb JD3.2 and Alexa fluor488 (Invitrogen) conjugated
Article Title: CaGdt1 plays a compensatory role for the calcium pump CaPmr1 in the regulation of calcium signaling and cell wall integrity signaling in Candida albicans
Article Snippet: BSA of 400 μg ml− 1 was added to the spheroplast mixture, and incubated for 20 min before mouse anti-HA antibodies was added at a dilution of 1:500. .. The mixture was incubated for 2 h, and washed twice with PBST before
Article Title: Design of novel small molecule base-pair recognizers of toxic CUG RNA transcripts characteristics of DM1
Article Snippet: .. Then, samples were incubated with anti-MBNL1 3A4 antibody (Santa Cruz Biotechnology) solution in PBS at room temperature for 1 h. After that samples were washed 3 times with PBS and incubated using
Article Title: RIM1/2-Mediated Facilitation of Cav1.4 Channel Opening Is Required for Ca2+-Stimulated Release in Mouse Rod Photoreceptors
Article Snippet: Two primary antibodies were added simultaneously over the first night (one rabbit IgG, anti-Cav, and the other mouse IgG, anti-Cre) and then anti-CtBP2 was added for the second overnight incubation. .. In most instances, rabbit IgGs were labeled with chicken anti-rabbit IgG-Alexa Fluor 488 (Invitrogen, ) and mouse IgGs were labeled with
Article Title: A Soluble Fragment of the Tumor Antigen BCL2-associated Athanogene 6 (BAG-6) Is Essential and Sufficient for Inhibition of NKp30 Receptor-dependent Cytotoxicity of Natural Killer Cells *
Article Snippet: The following antibodies were used: mouse monoclonal anti-polyhistidine tag HRP-conjugate and anti-human HRP-conjugate (both Sigma-Aldrich), anti-Strep-mAb classic HRP (IBA), anti-NKp30 clone p30-15 (kindly provided by Carsten Watzl), goat anti-mouse IgG1 APC conjugate (Life Technologies), and