Structured Review

Jackson Immuno goat anti mouse igg
Trib1 overexpression in B cells induces a reduction in the production of secreted form of IgG1. Total splenocytes from Trib1-ROSA and Trib1-ROSA Mb1Cre mice were stimulated with LPS/IL-4 for 72 h in vitro [with (+) or without (Ø) the addition of a protein transport inhibitor “GolgiStop™” for the last 8 h of culture], then stained for intracytoplasmic IgG1 after a step of membrane Ig blocking using an anti-murine <t>IgG</t> antibody, a step of fixation and permeabilization, and finally were analyzed by flow cytometry. (A) Percentage of <t>B220</t> + B cells stained for intracellular IgG1. (B) MFI of intracellular IgG1 staining on B220 + B cells. (C) A representative sample of each condition is shown. Each dot represents the result for one animal.
Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse igg/product/Jackson Immuno
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
goat anti mouse igg - by Bioz Stars, 2021-04
86/100 stars

Images

1) Product Images from "Trib1 Is Overexpressed in Systemic Lupus Erythematosus, While It Regulates Immunoglobulin Production in Murine B Cells"

Article Title: Trib1 Is Overexpressed in Systemic Lupus Erythematosus, While It Regulates Immunoglobulin Production in Murine B Cells

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2018.00373

Trib1 overexpression in B cells induces a reduction in the production of secreted form of IgG1. Total splenocytes from Trib1-ROSA and Trib1-ROSA Mb1Cre mice were stimulated with LPS/IL-4 for 72 h in vitro [with (+) or without (Ø) the addition of a protein transport inhibitor “GolgiStop™” for the last 8 h of culture], then stained for intracytoplasmic IgG1 after a step of membrane Ig blocking using an anti-murine IgG antibody, a step of fixation and permeabilization, and finally were analyzed by flow cytometry. (A) Percentage of B220 + B cells stained for intracellular IgG1. (B) MFI of intracellular IgG1 staining on B220 + B cells. (C) A representative sample of each condition is shown. Each dot represents the result for one animal.
Figure Legend Snippet: Trib1 overexpression in B cells induces a reduction in the production of secreted form of IgG1. Total splenocytes from Trib1-ROSA and Trib1-ROSA Mb1Cre mice were stimulated with LPS/IL-4 for 72 h in vitro [with (+) or without (Ø) the addition of a protein transport inhibitor “GolgiStop™” for the last 8 h of culture], then stained for intracytoplasmic IgG1 after a step of membrane Ig blocking using an anti-murine IgG antibody, a step of fixation and permeabilization, and finally were analyzed by flow cytometry. (A) Percentage of B220 + B cells stained for intracellular IgG1. (B) MFI of intracellular IgG1 staining on B220 + B cells. (C) A representative sample of each condition is shown. Each dot represents the result for one animal.

Techniques Used: Over Expression, Mouse Assay, In Vitro, Staining, Blocking Assay, Flow Cytometry, Cytometry

2) Product Images from "B Cell-Specific Expression of Ataxia-Telangiectasia Mutated Protein Kinase Promotes Chronic Gammaherpesvirus Infection"

Article Title: B Cell-Specific Expression of Ataxia-Telangiectasia Mutated Protein Kinase Promotes Chronic Gammaherpesvirus Infection

Journal: Journal of Virology

doi: 10.1128/JVI.01103-17

ATM deficiency in B cells attenuates MHV68-driven B cell differentiation. B-Cre-positive and -negative mice were either mock treated or infected intranasally with 10 4 PFU of MHV68. At 16 days postinfection (dpi), splenocytes were harvested and analyzed using flow cytometry. Each data point represents an individual mouse; data from 2 to 4 independent experiments were pooled. (A) Class-switched B cells were pregated on B220 + and identified as IgM − IgD − (a representative flow diagram is shown). Boxed areas identify immune populations of interest. (B and C) The frequencies (B) and the absolute numbers (C) of B220 + IgM − IgD − splenocytes were quantified. (D) Germinal center B cells were pregated on B220 + and further identified as CD95 + GL7 + (a representative flow diagram is shown). (E and F) Frequencies (E) and absolute numbers (F) of B220 + CD95 + GL7 + splenocytes. (G) Plasma cells were pregated on B220 + , further gated as IgM − IgD − , and identified for surface expression of CD138 + and for intracellular IgG + (representative flow diagrams are shown). (H and I) Frequencies (H) and absolute numbers (I) of B220 + IgM − IgD − CD138 + IgG + plasma cells. (J to M) Serum total immunoglobulin (J) and MHV68-specific antibodies (total [K], IgM [L], and IgG [M]) were measured by ELISA at 16 days post-mock treatment or -MHV68 infection; pooled data are shown. *, P
Figure Legend Snippet: ATM deficiency in B cells attenuates MHV68-driven B cell differentiation. B-Cre-positive and -negative mice were either mock treated or infected intranasally with 10 4 PFU of MHV68. At 16 days postinfection (dpi), splenocytes were harvested and analyzed using flow cytometry. Each data point represents an individual mouse; data from 2 to 4 independent experiments were pooled. (A) Class-switched B cells were pregated on B220 + and identified as IgM − IgD − (a representative flow diagram is shown). Boxed areas identify immune populations of interest. (B and C) The frequencies (B) and the absolute numbers (C) of B220 + IgM − IgD − splenocytes were quantified. (D) Germinal center B cells were pregated on B220 + and further identified as CD95 + GL7 + (a representative flow diagram is shown). (E and F) Frequencies (E) and absolute numbers (F) of B220 + CD95 + GL7 + splenocytes. (G) Plasma cells were pregated on B220 + , further gated as IgM − IgD − , and identified for surface expression of CD138 + and for intracellular IgG + (representative flow diagrams are shown). (H and I) Frequencies (H) and absolute numbers (I) of B220 + IgM − IgD − CD138 + IgG + plasma cells. (J to M) Serum total immunoglobulin (J) and MHV68-specific antibodies (total [K], IgM [L], and IgG [M]) were measured by ELISA at 16 days post-mock treatment or -MHV68 infection; pooled data are shown. *, P

Techniques Used: Cell Differentiation, Mouse Assay, Infection, Flow Cytometry, Cytometry, Expressing, Enzyme-linked Immunosorbent Assay

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Article Snippet: Proteins were electrophoretically transferred from gel slabs to nitrocellulose membranes for 90 min at 100 V. Membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline (137 mM NaCl, 25 mM Tris, 2.7 mM KCl, pH 7.4) containing 2% Tween-20 (TBS-T) for 60 min, then incubated with rabbit polyclonal IgGs that specifically recognize S1P1 (0.1 µg/ml, Affinity Bioreagents), S1P2 (EDG5, 0.2 µg/ml, Santa Cruz), or mouse monoclonal IgGs against S1P3 (EDG3, 0.1 µg/ml, Exalpha Biologicals) receptor subtypes. .. Following overnight incubation at 4°C, membranes were washed (3 × 10 min) with TBS-T, incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit or goat anti-mouse IgGs (40 ng/ml, Jackson Immunoresearch Laboratories) for 60 min at room temperature, and washed again with TBS-T (3 × 10 min). .. To visualize proteins, membranes were incubated with either ECL Advance (Amersham) or HyGLO (Denville Scientific) chemiluminescence reagents and exposed to X-ray film (Genesee Scientific).

other:

Article Title: The c-Jun and JunB transcription factors facilitate the transit of classical Hodgkin lymphoma tumour cells through G1
Article Snippet: Horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) were from Jackson ImmunoReseach Laboratories (West Grove, PA).

Enzyme-linked Immunosorbent Assay:

Article Title: Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Article Snippet: B Measurement of cytokines and chemokines B Site-Directed Mutagenesis B Purification of primary neutrophils B Expression and purification of isoleucine zipper TRAIL B TRAIL receptor complex and Caspase-8 immunopre- cipitations B Apoptosis Assays B Flow cytometry B Transfection and immunoblotting B RNA Interference B Generation of Caspase-8 deficient cell lines via CRISPR/Cas9 B Biotinylation of iz-TRAIL B Chemotaxis assays B Intracellular cytokine staining d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental Information includes six figures and one table and can be found with this article online at http://dx.doi.org/10.1016/j.molcel.2017.01.022. .. Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcg fragment specific Jackson Immunoresearch Europe #115-035-008, RRID: AB_2313585 Alexa Fluor 488 goat anti-mouse (H+L) Life Technologies (ThermoFisher Scientific) #A11029, RRID: AB_138404 Human IL-6 ELISA Duoset Bio-Techne (R systems) #DY206 Human IL-8 ELISA Duoset Bio-Techne (R systems) #DY208 Human CXCL1 ELISA Duoset Bio-Techne (R systems) #DY275 Human MIF ELISA Duoset Bio-Techne (R systems) #DY289 Human MCP-1 ELISA Duoset Bio-Techne (R systems) #DY279 Human GM-CSF ELISA Duoset Bio-Techne (R systems) #DY215 Mouse MIP-1 ELISA Duoset Bio-Techne (R systems) #DY450 Mouse KC ELISA Duoset Bio-Techne (R systems) #DY453 Chemicals, Peptides, and Recombinant Proteins poly-caspase inhibitor Z-VAD-FMK Bachem #N-1510.0005 poly-caspase inhibitor Q-VD-OPh ApexBio #A1901 Protein G-Sepharose Fast Flow Sigma Chemical #P3296 PR619 Sigma Chemical #SML0430 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma Chemical #I6758 Imidazole Sigma Chemical #I5513 biotinyl aminocaproic acid-N-hydroxysuccinimide ester (Biotin-NHS) Sigma Chemical #B2643 Kanamycin Sigma Chemical #60615 Amphicillin Sigma Chemical #A9518 Pfu DNA Polymerase Promega #M774A DpnI enzyme New England BioLabs (NEB) #R0176L Propidium iodide (PI) Sigma Chemical #P4170 Strepavidin-Sepharose High Performance GE Healthcare #17-5113-01 Protein A/G-PLUS-Agarose Santa-Cruz Biotechnology #sc-2003 SlowFade Molecular probes #S36937 cOmplete protease inhibitor cocktail Roche #04693116001 Amintra NiNTA Resin Expedeon #ANN0025 GeneJet SignaGen Laboratories #SL100488 SuperKillerTRAIL Adipogen #AG-40T-0002-C020 Recombinant TNFa Roche #11371843001 Experimental Models: Cell Lines Human: HeLa Martin Laboratory Cullen et al., 2013 Human: HCT116 ATCC #CCL-247 Human: Primary Prostate epithelial cells Lonza #CC-255 Human: HaCat Martin Laboratory Cullen et al., 2013 Human: THP-1 Martin Laboratory Cullen et al., 2015 Human: HT-29 Martin Laboratory Cullen et al., 2013 Human: HEK293T Martin Laboratory Cullen et al., 2013 Human: PancTU-1 Martin Laboratory N/A Mouse: 3LL Martin Laboratory N/A Bacteria: E.Coli DH5a Martin Laboratory N/A Bacteria: E.Coli Rosetta (DE3) pLysS Prof. Henning Walczak (UCL, UK) N/A Recombinant DNA Plasmid: pcDNA3-Caspase-8-HA Prof. .. Guy Salvesen (Sanford Burnham, USA) N/A Plasmid: pcDNA3-Caspase-8 C360A-HA Prof.

Recombinant:

Article Title: Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Article Snippet: B Measurement of cytokines and chemokines B Site-Directed Mutagenesis B Purification of primary neutrophils B Expression and purification of isoleucine zipper TRAIL B TRAIL receptor complex and Caspase-8 immunopre- cipitations B Apoptosis Assays B Flow cytometry B Transfection and immunoblotting B RNA Interference B Generation of Caspase-8 deficient cell lines via CRISPR/Cas9 B Biotinylation of iz-TRAIL B Chemotaxis assays B Intracellular cytokine staining d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental Information includes six figures and one table and can be found with this article online at http://dx.doi.org/10.1016/j.molcel.2017.01.022. .. Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcg fragment specific Jackson Immunoresearch Europe #115-035-008, RRID: AB_2313585 Alexa Fluor 488 goat anti-mouse (H+L) Life Technologies (ThermoFisher Scientific) #A11029, RRID: AB_138404 Human IL-6 ELISA Duoset Bio-Techne (R systems) #DY206 Human IL-8 ELISA Duoset Bio-Techne (R systems) #DY208 Human CXCL1 ELISA Duoset Bio-Techne (R systems) #DY275 Human MIF ELISA Duoset Bio-Techne (R systems) #DY289 Human MCP-1 ELISA Duoset Bio-Techne (R systems) #DY279 Human GM-CSF ELISA Duoset Bio-Techne (R systems) #DY215 Mouse MIP-1 ELISA Duoset Bio-Techne (R systems) #DY450 Mouse KC ELISA Duoset Bio-Techne (R systems) #DY453 Chemicals, Peptides, and Recombinant Proteins poly-caspase inhibitor Z-VAD-FMK Bachem #N-1510.0005 poly-caspase inhibitor Q-VD-OPh ApexBio #A1901 Protein G-Sepharose Fast Flow Sigma Chemical #P3296 PR619 Sigma Chemical #SML0430 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma Chemical #I6758 Imidazole Sigma Chemical #I5513 biotinyl aminocaproic acid-N-hydroxysuccinimide ester (Biotin-NHS) Sigma Chemical #B2643 Kanamycin Sigma Chemical #60615 Amphicillin Sigma Chemical #A9518 Pfu DNA Polymerase Promega #M774A DpnI enzyme New England BioLabs (NEB) #R0176L Propidium iodide (PI) Sigma Chemical #P4170 Strepavidin-Sepharose High Performance GE Healthcare #17-5113-01 Protein A/G-PLUS-Agarose Santa-Cruz Biotechnology #sc-2003 SlowFade Molecular probes #S36937 cOmplete protease inhibitor cocktail Roche #04693116001 Amintra NiNTA Resin Expedeon #ANN0025 GeneJet SignaGen Laboratories #SL100488 SuperKillerTRAIL Adipogen #AG-40T-0002-C020 Recombinant TNFa Roche #11371843001 Experimental Models: Cell Lines Human: HeLa Martin Laboratory Cullen et al., 2013 Human: HCT116 ATCC #CCL-247 Human: Primary Prostate epithelial cells Lonza #CC-255 Human: HaCat Martin Laboratory Cullen et al., 2013 Human: THP-1 Martin Laboratory Cullen et al., 2015 Human: HT-29 Martin Laboratory Cullen et al., 2013 Human: HEK293T Martin Laboratory Cullen et al., 2013 Human: PancTU-1 Martin Laboratory N/A Mouse: 3LL Martin Laboratory N/A Bacteria: E.Coli DH5a Martin Laboratory N/A Bacteria: E.Coli Rosetta (DE3) pLysS Prof. Henning Walczak (UCL, UK) N/A Recombinant DNA Plasmid: pcDNA3-Caspase-8-HA Prof. .. Guy Salvesen (Sanford Burnham, USA) N/A Plasmid: pcDNA3-Caspase-8 C360A-HA Prof.

Protease Inhibitor:

Article Title: Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Article Snippet: B Measurement of cytokines and chemokines B Site-Directed Mutagenesis B Purification of primary neutrophils B Expression and purification of isoleucine zipper TRAIL B TRAIL receptor complex and Caspase-8 immunopre- cipitations B Apoptosis Assays B Flow cytometry B Transfection and immunoblotting B RNA Interference B Generation of Caspase-8 deficient cell lines via CRISPR/Cas9 B Biotinylation of iz-TRAIL B Chemotaxis assays B Intracellular cytokine staining d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental Information includes six figures and one table and can be found with this article online at http://dx.doi.org/10.1016/j.molcel.2017.01.022. .. Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcg fragment specific Jackson Immunoresearch Europe #115-035-008, RRID: AB_2313585 Alexa Fluor 488 goat anti-mouse (H+L) Life Technologies (ThermoFisher Scientific) #A11029, RRID: AB_138404 Human IL-6 ELISA Duoset Bio-Techne (R systems) #DY206 Human IL-8 ELISA Duoset Bio-Techne (R systems) #DY208 Human CXCL1 ELISA Duoset Bio-Techne (R systems) #DY275 Human MIF ELISA Duoset Bio-Techne (R systems) #DY289 Human MCP-1 ELISA Duoset Bio-Techne (R systems) #DY279 Human GM-CSF ELISA Duoset Bio-Techne (R systems) #DY215 Mouse MIP-1 ELISA Duoset Bio-Techne (R systems) #DY450 Mouse KC ELISA Duoset Bio-Techne (R systems) #DY453 Chemicals, Peptides, and Recombinant Proteins poly-caspase inhibitor Z-VAD-FMK Bachem #N-1510.0005 poly-caspase inhibitor Q-VD-OPh ApexBio #A1901 Protein G-Sepharose Fast Flow Sigma Chemical #P3296 PR619 Sigma Chemical #SML0430 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma Chemical #I6758 Imidazole Sigma Chemical #I5513 biotinyl aminocaproic acid-N-hydroxysuccinimide ester (Biotin-NHS) Sigma Chemical #B2643 Kanamycin Sigma Chemical #60615 Amphicillin Sigma Chemical #A9518 Pfu DNA Polymerase Promega #M774A DpnI enzyme New England BioLabs (NEB) #R0176L Propidium iodide (PI) Sigma Chemical #P4170 Strepavidin-Sepharose High Performance GE Healthcare #17-5113-01 Protein A/G-PLUS-Agarose Santa-Cruz Biotechnology #sc-2003 SlowFade Molecular probes #S36937 cOmplete protease inhibitor cocktail Roche #04693116001 Amintra NiNTA Resin Expedeon #ANN0025 GeneJet SignaGen Laboratories #SL100488 SuperKillerTRAIL Adipogen #AG-40T-0002-C020 Recombinant TNFa Roche #11371843001 Experimental Models: Cell Lines Human: HeLa Martin Laboratory Cullen et al., 2013 Human: HCT116 ATCC #CCL-247 Human: Primary Prostate epithelial cells Lonza #CC-255 Human: HaCat Martin Laboratory Cullen et al., 2013 Human: THP-1 Martin Laboratory Cullen et al., 2015 Human: HT-29 Martin Laboratory Cullen et al., 2013 Human: HEK293T Martin Laboratory Cullen et al., 2013 Human: PancTU-1 Martin Laboratory N/A Mouse: 3LL Martin Laboratory N/A Bacteria: E.Coli DH5a Martin Laboratory N/A Bacteria: E.Coli Rosetta (DE3) pLysS Prof. Henning Walczak (UCL, UK) N/A Recombinant DNA Plasmid: pcDNA3-Caspase-8-HA Prof. .. Guy Salvesen (Sanford Burnham, USA) N/A Plasmid: pcDNA3-Caspase-8 C360A-HA Prof.

Plasmid Preparation:

Article Title: Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Article Snippet: B Measurement of cytokines and chemokines B Site-Directed Mutagenesis B Purification of primary neutrophils B Expression and purification of isoleucine zipper TRAIL B TRAIL receptor complex and Caspase-8 immunopre- cipitations B Apoptosis Assays B Flow cytometry B Transfection and immunoblotting B RNA Interference B Generation of Caspase-8 deficient cell lines via CRISPR/Cas9 B Biotinylation of iz-TRAIL B Chemotaxis assays B Intracellular cytokine staining d QUANTIFICATION AND STATISTICAL ANALYSIS SUPPLEMENTAL INFORMATION Supplemental Information includes six figures and one table and can be found with this article online at http://dx.doi.org/10.1016/j.molcel.2017.01.022. .. Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcg fragment specific Jackson Immunoresearch Europe #115-035-008, RRID: AB_2313585 Alexa Fluor 488 goat anti-mouse (H+L) Life Technologies (ThermoFisher Scientific) #A11029, RRID: AB_138404 Human IL-6 ELISA Duoset Bio-Techne (R systems) #DY206 Human IL-8 ELISA Duoset Bio-Techne (R systems) #DY208 Human CXCL1 ELISA Duoset Bio-Techne (R systems) #DY275 Human MIF ELISA Duoset Bio-Techne (R systems) #DY289 Human MCP-1 ELISA Duoset Bio-Techne (R systems) #DY279 Human GM-CSF ELISA Duoset Bio-Techne (R systems) #DY215 Mouse MIP-1 ELISA Duoset Bio-Techne (R systems) #DY450 Mouse KC ELISA Duoset Bio-Techne (R systems) #DY453 Chemicals, Peptides, and Recombinant Proteins poly-caspase inhibitor Z-VAD-FMK Bachem #N-1510.0005 poly-caspase inhibitor Q-VD-OPh ApexBio #A1901 Protein G-Sepharose Fast Flow Sigma Chemical #P3296 PR619 Sigma Chemical #SML0430 Isopropyl b-D-1-thiogalactopyranoside (IPTG) Sigma Chemical #I6758 Imidazole Sigma Chemical #I5513 biotinyl aminocaproic acid-N-hydroxysuccinimide ester (Biotin-NHS) Sigma Chemical #B2643 Kanamycin Sigma Chemical #60615 Amphicillin Sigma Chemical #A9518 Pfu DNA Polymerase Promega #M774A DpnI enzyme New England BioLabs (NEB) #R0176L Propidium iodide (PI) Sigma Chemical #P4170 Strepavidin-Sepharose High Performance GE Healthcare #17-5113-01 Protein A/G-PLUS-Agarose Santa-Cruz Biotechnology #sc-2003 SlowFade Molecular probes #S36937 cOmplete protease inhibitor cocktail Roche #04693116001 Amintra NiNTA Resin Expedeon #ANN0025 GeneJet SignaGen Laboratories #SL100488 SuperKillerTRAIL Adipogen #AG-40T-0002-C020 Recombinant TNFa Roche #11371843001 Experimental Models: Cell Lines Human: HeLa Martin Laboratory Cullen et al., 2013 Human: HCT116 ATCC #CCL-247 Human: Primary Prostate epithelial cells Lonza #CC-255 Human: HaCat Martin Laboratory Cullen et al., 2013 Human: THP-1 Martin Laboratory Cullen et al., 2015 Human: HT-29 Martin Laboratory Cullen et al., 2013 Human: HEK293T Martin Laboratory Cullen et al., 2013 Human: PancTU-1 Martin Laboratory N/A Mouse: 3LL Martin Laboratory N/A Bacteria: E.Coli DH5a Martin Laboratory N/A Bacteria: E.Coli Rosetta (DE3) pLysS Prof. Henning Walczak (UCL, UK) N/A Recombinant DNA Plasmid: pcDNA3-Caspase-8-HA Prof. .. Guy Salvesen (Sanford Burnham, USA) N/A Plasmid: pcDNA3-Caspase-8 C360A-HA Prof.

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    Jackson Immuno goat anti mouse igg m
    N-WASP is activated following WASP activation upon antigen stimulation. (A–D) TIRFM and IRM analysis of pWASP and pN-WASP in the B-cell contact zone of mouse splenic B cells and human PBMC B cells that were incubated with membrane-tethered AF546–mB-Fab′–anti-mouse or human <t>IgG+M</t> at 37°C for indicated times. (E and G) The MFI of pWASP or pN-WASP in the B-cell contact zone was quantified using TIRFM images and Andor iQ software. (F and H) The MFI of pWASP or pN-WASP in mouse splenic and human PBMC B cells incubated with soluble Fab′–anti-IgG+M plus streptavidin at 37°C for indicated times were analyzed by flow <t>cytometry.</t> Shown are representative images and the average MFI (±SD) from three independent experiments. Bar, 2.5 µm.
    Goat Anti Mouse Igg M, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg m/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg m - by Bioz Stars, 2021-04
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    93
    Jackson Immuno goat antimouse igg h l antibody
    Physicochemical properties of the MERS‐CoV nanoparticle vaccine. A) Size and zeta potential of adjuvant‐loaded nanoparticles before and after the MERS‐CoV RBD antigen conjugation. B) Estimated numbers of MERS‐CoV RBD antigens on each PLGA hollow nanoparticle. Nanoparticle‐attached antigens were calculated by directly quantifying protein contents on nanoparticles after conjugation reaction using the BCA protein assay. C) Loading of cdGMP in synthetic hollow nanoparticles before and after conjugation with recombinant MERS‐CoV RBD antigens. D) Cryo‐electron microscopy and E) transmission electron microscopy of MERS‐CoV RBD coated nanoparticles. F) Immunogold staining of the MERS‐CoV RBD conjugated nanoparticle with anti‐His tag and goat <t>antimouse</t> <t>IgG</t> antibodies followed by transmission electron microscopy. Error bars represent mean ± SEM ( N = 3).
    Goat Antimouse Igg H L Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antimouse igg h l antibody/product/Jackson Immuno
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    94
    Jackson Immuno fitc conjugated goat anti mouse antibody
    Cell surface expression of the mutant receptors. (A) Stable cell lines derived from G418 selection of transfected J cells expressing the wild-type or mutant murine nectin1 receptors were reacted with the rat 48-14 hybridoma supernatant followed by anti-rat IgG conjugated with <t>FITC.</t> Stable cell lines expressing human wild-type or mutant receptors were reacted with MAb <t>CK35</t> followed by anti-mouse IgG conjugated with FITC. Selected examples of fluorescence analyzed in a Becton Dickinson flow cytometer are shown. (B) PrV entry activity of wild-type and mutant receptors. Stable cell lines expressing the indicated constructs were exposed to increasing amounts of PrV-LacZ. After 16 h, β-Gal activity was measured with ONPG as a substrate and absorbance was monitored at 405 nm.
    Fitc Conjugated Goat Anti Mouse Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Jackson Immuno alkaline phosphatase conjugated goat anti mouse igm
    Analysis of purity and DPPC-reactivity of <t>IgM</t> purified from anti-phosphocholine antibodies-containing sera . The IgM fraction purified from sera of BALB/c mice immunized with empty Lp DPPC was analyzed by SDS-PAGE and Western blot. (A) Silver-stained SDS-PAGE in 12% acrylamide under reducing conditions. The presence of (B) IgM and (C) <t>IgG</t> in each sample was determined by Western blot using alkaline phosphatase-conjugated goat anti-mouse IgM (μ-chain-specific) and anti-mouse IgG (whole molecule) antibodies, respectively. MW: molecular weight markers; IgM/IgG: standards. 1: eluted fraction from the IgM affinity chromatography applied into the protein G chromatography; 2: unbound fraction; and 3: eluted fraction. Recognition of DPPC by IgM from sera of animals immunized with Lp DPPC (IgM DPPC ) or Lp DPPG (IgM DPPG ), and by an irrelevant IgM (IgM irrelev ) was assessed by (D) ELISA and (E) flow cytometry. In (E) , representative histograms of Lp DPPC/OVA opsonized with IgM DPPC (black line), IgM DPPG (gray line), IgM irrelev (dotted line), and Lp DPPC/OVA alone (filled) are shown.
    Alkaline Phosphatase Conjugated Goat Anti Mouse Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    N-WASP is activated following WASP activation upon antigen stimulation. (A–D) TIRFM and IRM analysis of pWASP and pN-WASP in the B-cell contact zone of mouse splenic B cells and human PBMC B cells that were incubated with membrane-tethered AF546–mB-Fab′–anti-mouse or human IgG+M at 37°C for indicated times. (E and G) The MFI of pWASP or pN-WASP in the B-cell contact zone was quantified using TIRFM images and Andor iQ software. (F and H) The MFI of pWASP or pN-WASP in mouse splenic and human PBMC B cells incubated with soluble Fab′–anti-IgG+M plus streptavidin at 37°C for indicated times were analyzed by flow cytometry. Shown are representative images and the average MFI (±SD) from three independent experiments. Bar, 2.5 µm.

    Journal: PLoS Biology

    Article Title: N-WASP Is Essential for the Negative Regulation of B Cell Receptor Signaling

    doi: 10.1371/journal.pbio.1001704

    Figure Lengend Snippet: N-WASP is activated following WASP activation upon antigen stimulation. (A–D) TIRFM and IRM analysis of pWASP and pN-WASP in the B-cell contact zone of mouse splenic B cells and human PBMC B cells that were incubated with membrane-tethered AF546–mB-Fab′–anti-mouse or human IgG+M at 37°C for indicated times. (E and G) The MFI of pWASP or pN-WASP in the B-cell contact zone was quantified using TIRFM images and Andor iQ software. (F and H) The MFI of pWASP or pN-WASP in mouse splenic and human PBMC B cells incubated with soluble Fab′–anti-IgG+M plus streptavidin at 37°C for indicated times were analyzed by flow cytometry. Shown are representative images and the average MFI (±SD) from three independent experiments. Bar, 2.5 µm.

    Article Snippet: Flow cytometry B cells were incubated with biotinylated F(ab′)2 -goat anti-mouse IgG+M (10 µg/ml; Jackson ImmunoResearch) at 4°C and chased at 37°C .

    Techniques: Activation Assay, Incubation, Software, Flow Cytometry, Cytometry

    Physicochemical properties of the MERS‐CoV nanoparticle vaccine. A) Size and zeta potential of adjuvant‐loaded nanoparticles before and after the MERS‐CoV RBD antigen conjugation. B) Estimated numbers of MERS‐CoV RBD antigens on each PLGA hollow nanoparticle. Nanoparticle‐attached antigens were calculated by directly quantifying protein contents on nanoparticles after conjugation reaction using the BCA protein assay. C) Loading of cdGMP in synthetic hollow nanoparticles before and after conjugation with recombinant MERS‐CoV RBD antigens. D) Cryo‐electron microscopy and E) transmission electron microscopy of MERS‐CoV RBD coated nanoparticles. F) Immunogold staining of the MERS‐CoV RBD conjugated nanoparticle with anti‐His tag and goat antimouse IgG antibodies followed by transmission electron microscopy. Error bars represent mean ± SEM ( N = 3).

    Journal: Advanced Functional Materials

    Article Title: Viromimetic STING Agonist‐Loaded Hollow Polymeric Nanoparticles for Safe and Effective Vaccination against Middle East Respiratory Syndrome Coronavirus, Viromimetic STING Agonist‐Loaded Hollow Polymeric Nanoparticles for Safe and Effective Vaccination against Middle East Respiratory Syndrome Coronavirus

    doi: 10.1002/adfm.201807616

    Figure Lengend Snippet: Physicochemical properties of the MERS‐CoV nanoparticle vaccine. A) Size and zeta potential of adjuvant‐loaded nanoparticles before and after the MERS‐CoV RBD antigen conjugation. B) Estimated numbers of MERS‐CoV RBD antigens on each PLGA hollow nanoparticle. Nanoparticle‐attached antigens were calculated by directly quantifying protein contents on nanoparticles after conjugation reaction using the BCA protein assay. C) Loading of cdGMP in synthetic hollow nanoparticles before and after conjugation with recombinant MERS‐CoV RBD antigens. D) Cryo‐electron microscopy and E) transmission electron microscopy of MERS‐CoV RBD coated nanoparticles. F) Immunogold staining of the MERS‐CoV RBD conjugated nanoparticle with anti‐His tag and goat antimouse IgG antibodies followed by transmission electron microscopy. Error bars represent mean ± SEM ( N = 3).

    Article Snippet: For nanoparticle visualization, negative staining was performed with uranyl acetate and immunogold staining was performed using mouse anti‐His tag antibody (Roche) and goat antimouse IgG (H+L) antibody conjugated with 6 nm colloidal gold (Jackson ImmunoResearch, West Grove, PA).

    Techniques: Conjugation Assay, Bicinchoninic Acid Protein Assay, Recombinant, Electron Microscopy, Transmission Assay, Staining

    Cell surface expression of the mutant receptors. (A) Stable cell lines derived from G418 selection of transfected J cells expressing the wild-type or mutant murine nectin1 receptors were reacted with the rat 48-14 hybridoma supernatant followed by anti-rat IgG conjugated with FITC. Stable cell lines expressing human wild-type or mutant receptors were reacted with MAb CK35 followed by anti-mouse IgG conjugated with FITC. Selected examples of fluorescence analyzed in a Becton Dickinson flow cytometer are shown. (B) PrV entry activity of wild-type and mutant receptors. Stable cell lines expressing the indicated constructs were exposed to increasing amounts of PrV-LacZ. After 16 h, β-Gal activity was measured with ONPG as a substrate and absorbance was monitored at 405 nm.

    Journal: Journal of Virology

    Article Title: Substitution in the Murine Nectin1 Receptor of a Single Conserved Amino Acid at a Position Distal from the Herpes Simplex Virus gD Binding Site Confers High-Affinity Binding to gD †

    doi: 10.1128/JVI.76.11.5463-5471.2002

    Figure Lengend Snippet: Cell surface expression of the mutant receptors. (A) Stable cell lines derived from G418 selection of transfected J cells expressing the wild-type or mutant murine nectin1 receptors were reacted with the rat 48-14 hybridoma supernatant followed by anti-rat IgG conjugated with FITC. Stable cell lines expressing human wild-type or mutant receptors were reacted with MAb CK35 followed by anti-mouse IgG conjugated with FITC. Selected examples of fluorescence analyzed in a Becton Dickinson flow cytometer are shown. (B) PrV entry activity of wild-type and mutant receptors. Stable cell lines expressing the indicated constructs were exposed to increasing amounts of PrV-LacZ. After 16 h, β-Gal activity was measured with ONPG as a substrate and absorbance was monitored at 405 nm.

    Article Snippet: For cell surface determination of mutant receptors, stable cell lines were stained with anti-murine nectin1 rat MAb 48-14 (undiluted hybridoma supernatant) followed by fluorescein isothiocyanate (FITC)-conjugated rabbit anti-rat IgG (Sigma) at 1:300 or with MAb CK35 directed to human nectin1 (ascites, 1:100) followed by FITC-conjugated goat anti-mouse antibody (Jackson Immunoresearch Laboratories) at 1:100; the cell lines were then read in a Beckman Coulter FACScan flow cytometer.

    Techniques: Expressing, Mutagenesis, Stable Transfection, Derivative Assay, Selection, Transfection, Fluorescence, Flow Cytometry, Cytometry, Activity Assay, Construct

    Analysis of purity and DPPC-reactivity of IgM purified from anti-phosphocholine antibodies-containing sera . The IgM fraction purified from sera of BALB/c mice immunized with empty Lp DPPC was analyzed by SDS-PAGE and Western blot. (A) Silver-stained SDS-PAGE in 12% acrylamide under reducing conditions. The presence of (B) IgM and (C) IgG in each sample was determined by Western blot using alkaline phosphatase-conjugated goat anti-mouse IgM (μ-chain-specific) and anti-mouse IgG (whole molecule) antibodies, respectively. MW: molecular weight markers; IgM/IgG: standards. 1: eluted fraction from the IgM affinity chromatography applied into the protein G chromatography; 2: unbound fraction; and 3: eluted fraction. Recognition of DPPC by IgM from sera of animals immunized with Lp DPPC (IgM DPPC ) or Lp DPPG (IgM DPPG ), and by an irrelevant IgM (IgM irrelev ) was assessed by (D) ELISA and (E) flow cytometry. In (E) , representative histograms of Lp DPPC/OVA opsonized with IgM DPPC (black line), IgM DPPG (gray line), IgM irrelev (dotted line), and Lp DPPC/OVA alone (filled) are shown.

    Journal: Frontiers in Immunology

    Article Title: Phosphocholine-Specific Antibodies Improve T-Dependent Antibody Responses against OVA Encapsulated into Phosphatidylcholine-Containing Liposomes

    doi: 10.3389/fimmu.2016.00374

    Figure Lengend Snippet: Analysis of purity and DPPC-reactivity of IgM purified from anti-phosphocholine antibodies-containing sera . The IgM fraction purified from sera of BALB/c mice immunized with empty Lp DPPC was analyzed by SDS-PAGE and Western blot. (A) Silver-stained SDS-PAGE in 12% acrylamide under reducing conditions. The presence of (B) IgM and (C) IgG in each sample was determined by Western blot using alkaline phosphatase-conjugated goat anti-mouse IgM (μ-chain-specific) and anti-mouse IgG (whole molecule) antibodies, respectively. MW: molecular weight markers; IgM/IgG: standards. 1: eluted fraction from the IgM affinity chromatography applied into the protein G chromatography; 2: unbound fraction; and 3: eluted fraction. Recognition of DPPC by IgM from sera of animals immunized with Lp DPPC (IgM DPPC ) or Lp DPPG (IgM DPPG ), and by an irrelevant IgM (IgM irrelev ) was assessed by (D) ELISA and (E) flow cytometry. In (E) , representative histograms of Lp DPPC/OVA opsonized with IgM DPPC (black line), IgM DPPG (gray line), IgM irrelev (dotted line), and Lp DPPC/OVA alone (filled) are shown.

    Article Snippet: Polyacrylamide gel electrophoresis (SDS-PAGE) ( ) and Western blotting analysis were performed to assess the purity of samples, using alkaline phosphatase-conjugated goat anti-mouse IgM (μ-chain-specific) and anti-mouse IgG (whole molecule) antibodies (Jackson ImmunoResearch), respectively.

    Techniques: Purification, Mouse Assay, SDS Page, Western Blot, Staining, Molecular Weight, Affinity Chromatography, Chromatography, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry