goat anti mouse igg h l hrp  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Goat Anti Mouse IgG H L HRP
    Description:

    Catalog Number:
    AB205719
    Price:
    None
    Buy from Supplier


    Structured Review

    Abcam goat anti mouse igg h l hrp

    https://www.bioz.com/result/goat anti mouse igg h l hrp/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l hrp - by Bioz Stars, 2021-04
    97/100 stars

    Images

    Related Articles

    DNA Extraction:

    Article Title: Study on the association of the polymorphism of HLA-II gene with leukemia
    Article Snippet: Main instruments and reagents The main instruments used were PCR, refrigerated centrifuge and gel imaging instrument (all from Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. The main reagents were DNA extraction and enzyme-linked immunosorbent assay (ELISA) kits (both from Qiagen, Hilden, Germany), and HLA goat anti-mouse first antibody (cat. no. ab6789; dilution, 1:1,250; Abcam, Cambridge, MA, USA) and PCR reagents (Takara Bio, Dalian, China), SSO-HLA gene type kit (Dynal, Wirral, UK). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Study on the association of the polymorphism of HLA-II gene with leukemia
    Article Snippet: Main instruments and reagents The main instruments used were PCR, refrigerated centrifuge and gel imaging instrument (all from Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. The main reagents were DNA extraction and enzyme-linked immunosorbent assay (ELISA) kits (both from Qiagen, Hilden, Germany), and HLA goat anti-mouse first antibody (cat. no. ab6789; dilution, 1:1,250; Abcam, Cambridge, MA, USA) and PCR reagents (Takara Bio, Dalian, China), SSO-HLA gene type kit (Dynal, Wirral, UK). ..

    Polymerase Chain Reaction:

    Article Title: Study on the association of the polymorphism of HLA-II gene with leukemia
    Article Snippet: Main instruments and reagents The main instruments used were PCR, refrigerated centrifuge and gel imaging instrument (all from Bio-Rad Laboratories, Inc., Hercules, CA, USA). .. The main reagents were DNA extraction and enzyme-linked immunosorbent assay (ELISA) kits (both from Qiagen, Hilden, Germany), and HLA goat anti-mouse first antibody (cat. no. ab6789; dilution, 1:1,250; Abcam, Cambridge, MA, USA) and PCR reagents (Takara Bio, Dalian, China), SSO-HLA gene type kit (Dynal, Wirral, UK). ..

    SDS Page:

    Article Title: The plasminogen binding protein PbsP is required for brain invasion by hypervirulent CC17 Group B streptococci
    Article Snippet: Cell wall extracts and immunoblots Cell wall extracts were obtained by mutanolysin extraction in a hypertonic sucrose buffer and used for Western blot analyses as described previously , . .. Briefly, 10 µg of cell wall proteins were run on polyacrylamide gels (SDS-PAGE), transferred to a nitrocellulose membrane and PbsP was visualized by chemi-luminescence after incubation with mouse anti-PbsP serum and horseradish peroxidase conjugated goat anti mouse IgG (Abcam ab6789). .. Mouse meningitis model The CC17 BM110 strain and the deletion mutants were used to induce infection in 8-week-old CD1 female mice, as previously described , .

    Incubation:

    Article Title: The plasminogen binding protein PbsP is required for brain invasion by hypervirulent CC17 Group B streptococci
    Article Snippet: Cell wall extracts and immunoblots Cell wall extracts were obtained by mutanolysin extraction in a hypertonic sucrose buffer and used for Western blot analyses as described previously , . .. Briefly, 10 µg of cell wall proteins were run on polyacrylamide gels (SDS-PAGE), transferred to a nitrocellulose membrane and PbsP was visualized by chemi-luminescence after incubation with mouse anti-PbsP serum and horseradish peroxidase conjugated goat anti mouse IgG (Abcam ab6789). .. Mouse meningitis model The CC17 BM110 strain and the deletion mutants were used to induce infection in 8-week-old CD1 female mice, as previously described , .

    Article Title: Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing *
    Article Snippet: The RNA immunoprecipitation assay was performed using the protocol of Peritz et al. ( ). .. Briefly, HSV-1-infected HeLa cell lysates were incubated with the anti-SRSF2 antibody (Abcam, ab11826) and IgG antibody (Abcam, ab205719) at 4 °C overnight. .. RNA enriched from the immunoprecipitation was retro-transcribed for the real-time PCR.

    Article Title: Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing *
    Article Snippet: Briefly, the cells were fixed with 1% formaldehyde and sonicated to shear the DNA to an average fragment size of 200–1000 bp. .. After centrifugation, the supernatants were incubated with the SRSF2 antibody (Abcam, ab11826) and IgG antibody (Abcam, ab205719). .. Chromatin DNA was purified with the Dynabeads Protein G kit (Invitrogen, 10004D) and subjected to real-time PCR.

    Centrifugation:

    Article Title: Serine/Arginine-rich Splicing Factor 2 Modulates Herpes Simplex Virus Type 1 Replication via Regulating Viral Gene Transcriptional Activity and Pre-mRNA Splicing *
    Article Snippet: Briefly, the cells were fixed with 1% formaldehyde and sonicated to shear the DNA to an average fragment size of 200–1000 bp. .. After centrifugation, the supernatants were incubated with the SRSF2 antibody (Abcam, ab11826) and IgG antibody (Abcam, ab205719). .. Chromatin DNA was purified with the Dynabeads Protein G kit (Invitrogen, 10004D) and subjected to real-time PCR.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Abcam goat anti iga
    Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) <t>IgA</t> chimeric antibodies with V1, Nb23 and Nb5, (C) <t>IgY</t> chimeric antibodies with V1, Nb23 and Nb5.
    Goat Anti Iga, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti iga/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti iga - by Bioz Stars, 2021-04
    96/100 stars
      Buy from Supplier

    97
    Abcam goat anti mouse igg h l hrp antibody
    Panel a : The levels of E7-specific IgG and SIgA antibodies in serum (left) and vaginal discharges (right), respectively, on day 41; the sera and vaginal discharge samples were tested by indirect ELISA using recombinant human papillomavirus type 16 protein E7 as the coating antigen. Goat-anti-mouse IgG-H L <t>(HRP)</t> antibody and Goat Anti-Mouse IgA alpha chain (HRP) were used as a secondary antibody. The absorbance of each microwell was read on a spectrophotometer at 450 nm. Error bars represent the mean ± standard error value of each group ( P
    Goat Anti Mouse Igg H L Hrp Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg h l hrp antibody/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l hrp antibody - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    94
    Abcam goat anti mouse horseradish peroxidase labeled secondary antibodies
    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using <t>mouse</t> <t>anti-hITF</t> antibodies and <t>goat</t> anti-mouse <t>TRITC-labeled</t> <t>secondary</t> antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.
    Goat Anti Mouse Horseradish Peroxidase Labeled Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse horseradish peroxidase labeled secondary antibodies/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse horseradish peroxidase labeled secondary antibodies - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    iga  (Abcam)
    95
    Abcam iga
    Comparison of the intestinal villi of standard chow diet (SCD) and high-fat-diet (HFD) fed 20-week-old mice. Paraffin-embedded tissue serial sections were obtained from each diet model. HE-staining of villi in the small intestines from standard chow diet (SCD)-fed mice showed regularly aligned simple columnar epithelium ( a , b ). HE-staining of tissue sections from high-fat-diet (HFD)-fed mice revealed vacuoles in monolayer columnar epithelial cells ( c , white arrows in e ). HE-staining of tissues from SCD and HFD-fed mice showed red blood cells (arrowheads in b , d ). <t>IgA</t> immunoreactivity in SCD-fed mice was detected in the cytoplasm of epithelial cells ( g ), and many IgA-immunopositive cells were also observed in the whole lamina propria of intestinal villi ( f , black arrows in h ). Meanwhile, 20-week-old HFD-fed mice exhibited IgA immunoreactivity localized on the bottom of the lamina propria ( i , j ), and IgA-immunopositive cells (black arrows in k ) were further reduced compared to those in SCD-fed mice. In both diet models, IgM and IgG1 immunoreactivity were observed in blood vessels (white arrowheads in m , s , p , v ) and Ig-immunopositive cells (arrows in n , q , t , w ). The inset also indicates immuno-controls ( f , i , l , o , r , u ).
    Iga, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iga/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iga - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

    Image Search Results


    Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Expressing, Positive Control, Molecular Weight

    Expression of chimeric antibodies in transgenic A . thaliana seeds. Extracts of T2 seeds were coated and analysed by ELISA. The presence of chimeric antibodies in the extracts was tested, using anti-chicken IgA or IgY conjugated to HRP. As a negative control, extract of wild-type A . thaliana seeds was used. The results of seed extracts from A . thaliana plants transformed with Nb5-IgA, Nb23-IgA, V1-IgA, Nb5-IgY, Nb23-IgY and V1-IgY are shown.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Expression of chimeric antibodies in transgenic A . thaliana seeds. Extracts of T2 seeds were coated and analysed by ELISA. The presence of chimeric antibodies in the extracts was tested, using anti-chicken IgA or IgY conjugated to HRP. As a negative control, extract of wild-type A . thaliana seeds was used. The results of seed extracts from A . thaliana plants transformed with Nb5-IgA, Nb23-IgA, V1-IgA, Nb5-IgY, Nb23-IgY and V1-IgY are shown.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Expressing, Transgenic Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Transformation Assay

    Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates. (A, B, C, J, K, L) C . jejuni strain KC40, (D, E, F, M, N, O) C . jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C . coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E . coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates. (A, B, C, J, K, L) C . jejuni strain KC40, (D, E, F, M, N, O) C . jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C . coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E . coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Binding Assay, Negative Control, Microscopy

    Binding of chimeric antibodies to C . jejuni KC40 bacteria and purified MOMP. KC40 bacteria and MOMP (1 μ g/ml) were coated in an ELISA plate. Subsequently, the interaction of twofold serial dilutions of the seed extracts was assessed. Therefore, anti-IgA and anti-IgY antibodies, conjugated to HRP, were used for the detection of chimeric antibodies, bound to the bacteria or to the purified native MOMP antigen. As a negative control, the binding of extracts of wild-type A . thaliana seeds with the bacteria and MOMP was measured. (A) Nb5-IgA, (B) Nb23-IgA, (C) Nb5-IgY and (D) Nb23-IgY. The error bars correspond to the standard deviations.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Binding of chimeric antibodies to C . jejuni KC40 bacteria and purified MOMP. KC40 bacteria and MOMP (1 μ g/ml) were coated in an ELISA plate. Subsequently, the interaction of twofold serial dilutions of the seed extracts was assessed. Therefore, anti-IgA and anti-IgY antibodies, conjugated to HRP, were used for the detection of chimeric antibodies, bound to the bacteria or to the purified native MOMP antigen. As a negative control, the binding of extracts of wild-type A . thaliana seeds with the bacteria and MOMP was measured. (A) Nb5-IgA, (B) Nb23-IgA, (C) Nb5-IgY and (D) Nb23-IgY. The error bars correspond to the standard deviations.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Binding Assay, Purification, Enzyme-linked Immunosorbent Assay, Negative Control

    Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Expressing, SDS Page, Staining, Western Blot, Transformation Assay, Construct, Plasmid Preparation, Negative Control

    Interaction of chimeric antibodies in seed extract from homozygous plants with their antigen. SDS-PAGE and a western blot were performed on purified MOMP under non-denaturing conditions. (A) SDS-PAGE with purified native MOMP stained with Coomassie blue dye. The results of the western blot confirm the interactions of (B) Nb23-IgA14D and (C) Nb23-IgY12C, with MOMP. (D) Wild-type extract was used as a negative control. The western blot was developed with anti-IgA or anti-IgY antibodies conjugated to HRP.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Interaction of chimeric antibodies in seed extract from homozygous plants with their antigen. SDS-PAGE and a western blot were performed on purified MOMP under non-denaturing conditions. (A) SDS-PAGE with purified native MOMP stained with Coomassie blue dye. The results of the western blot confirm the interactions of (B) Nb23-IgA14D and (C) Nb23-IgY12C, with MOMP. (D) Wild-type extract was used as a negative control. The western blot was developed with anti-IgA or anti-IgY antibodies conjugated to HRP.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: SDS Page, Western Blot, Purification, Staining, Negative Control

    Panel a : The levels of E7-specific IgG and SIgA antibodies in serum (left) and vaginal discharges (right), respectively, on day 41; the sera and vaginal discharge samples were tested by indirect ELISA using recombinant human papillomavirus type 16 protein E7 as the coating antigen. Goat-anti-mouse IgG-H L (HRP) antibody and Goat Anti-Mouse IgA alpha chain (HRP) were used as a secondary antibody. The absorbance of each microwell was read on a spectrophotometer at 450 nm. Error bars represent the mean ± standard error value of each group ( P

    Journal: BMC Biotechnology

    Article Title: Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis

    doi: 10.1186/s12896-019-0499-5

    Figure Lengend Snippet: Panel a : The levels of E7-specific IgG and SIgA antibodies in serum (left) and vaginal discharges (right), respectively, on day 41; the sera and vaginal discharge samples were tested by indirect ELISA using recombinant human papillomavirus type 16 protein E7 as the coating antigen. Goat-anti-mouse IgG-H L (HRP) antibody and Goat Anti-Mouse IgA alpha chain (HRP) were used as a secondary antibody. The absorbance of each microwell was read on a spectrophotometer at 450 nm. Error bars represent the mean ± standard error value of each group ( P

    Article Snippet: They were then washed and blotted in 100 μL of goat-anti-mouse IgG-H & L (HRP) antibody (ab6789; Abcam, Canada; 1:1000 dilution) and goat Anti-Mouse IgA alpha chain (HRP) (ab97235; Abcam, Canada; 1:1000 dilution) at room temperature for 1 h. Next, the color was created with 3,3′,5,5′,-tetramethylbenzidine (TMB) for 10 min at 37 °C.

    Techniques: Indirect ELISA, Recombinant, Spectrophotometry

    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Journal: PLoS ONE

    Article Title: Recombinant Adenovirus-Mediated Intestinal Trefoil Factor Gene Therapy for Burn-Induced Intestinal Mucosal Injury

    doi: 10.1371/journal.pone.0062429

    Figure Lengend Snippet: Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Article Snippet: Mouse anti-hITF antibodies and goat anti-mouse horseradish peroxidase-labeled secondary antibodies were purchased from Abcom (Cambridge, MA, USA).

    Techniques: Infection, Labeling, Staining

    Comparison of the intestinal villi of standard chow diet (SCD) and high-fat-diet (HFD) fed 20-week-old mice. Paraffin-embedded tissue serial sections were obtained from each diet model. HE-staining of villi in the small intestines from standard chow diet (SCD)-fed mice showed regularly aligned simple columnar epithelium ( a , b ). HE-staining of tissue sections from high-fat-diet (HFD)-fed mice revealed vacuoles in monolayer columnar epithelial cells ( c , white arrows in e ). HE-staining of tissues from SCD and HFD-fed mice showed red blood cells (arrowheads in b , d ). IgA immunoreactivity in SCD-fed mice was detected in the cytoplasm of epithelial cells ( g ), and many IgA-immunopositive cells were also observed in the whole lamina propria of intestinal villi ( f , black arrows in h ). Meanwhile, 20-week-old HFD-fed mice exhibited IgA immunoreactivity localized on the bottom of the lamina propria ( i , j ), and IgA-immunopositive cells (black arrows in k ) were further reduced compared to those in SCD-fed mice. In both diet models, IgM and IgG1 immunoreactivity were observed in blood vessels (white arrowheads in m , s , p , v ) and Ig-immunopositive cells (arrows in n , q , t , w ). The inset also indicates immuno-controls ( f , i , l , o , r , u ).

    Journal: International Journal of Molecular Sciences

    Article Title: High-Fat Diet and Age-Dependent Effects of IgA-Bearing Cell Populations in the Small Intestinal Lamina Propria in Mice

    doi: 10.3390/ijms22031165

    Figure Lengend Snippet: Comparison of the intestinal villi of standard chow diet (SCD) and high-fat-diet (HFD) fed 20-week-old mice. Paraffin-embedded tissue serial sections were obtained from each diet model. HE-staining of villi in the small intestines from standard chow diet (SCD)-fed mice showed regularly aligned simple columnar epithelium ( a , b ). HE-staining of tissue sections from high-fat-diet (HFD)-fed mice revealed vacuoles in monolayer columnar epithelial cells ( c , white arrows in e ). HE-staining of tissues from SCD and HFD-fed mice showed red blood cells (arrowheads in b , d ). IgA immunoreactivity in SCD-fed mice was detected in the cytoplasm of epithelial cells ( g ), and many IgA-immunopositive cells were also observed in the whole lamina propria of intestinal villi ( f , black arrows in h ). Meanwhile, 20-week-old HFD-fed mice exhibited IgA immunoreactivity localized on the bottom of the lamina propria ( i , j ), and IgA-immunopositive cells (black arrows in k ) were further reduced compared to those in SCD-fed mice. In both diet models, IgM and IgG1 immunoreactivity were observed in blood vessels (white arrowheads in m , s , p , v ) and Ig-immunopositive cells (arrows in n , q , t , w ). The inset also indicates immuno-controls ( f , i , l , o , r , u ).

    Article Snippet: Then, the immunostained sections were incubated with horseradish peroxidase-conjugated donkey anti-goat (Goat IgG whole molecule, 1/400 dilution, Abcam Inc., Cambridge, MA, USA, ab6667) for IgA and -IgG1 or goat anti-rabbit IgG (Rabbit IgG whole molecule, 1/400 dilution, Abcam Inc., Cambridge, MA, USA, ab7171) for IgM, at room temperature for 1 h and finally visualized with diaminobenzidine (DAB) in a buffer solution containing hydrogen peroxide for 3 or 5 min.

    Techniques: Mouse Assay, Staining

    The number of Ig-immunopositive cells in the lamina propria of small intestinal villi. All cells in the whole lamina propria in the small intestinal villi were significantly different between 12-week and 20-week-old SCD-fed mice and between 12-week and 20-week-old HFD-fed mice ( a ). This trend was similar for all cells in the bottom ( b ) and middle ( c ) sections, but not for the top section ( d ). IgA-immunopositive cells in the whole area ( e ) were significantly different between 12- and 20-week-old SCD-fed mice and between SCD and HFD-fed 20-week-old mice. This trend was confirmed in all IgA-immunopositive cell comparisons, in the bottom ( f ), middle ( g ), and top ( h ) sections. IgM-immunopositive cells were significantly different between HFD and SCD-fed 12-week and 20-week-old mice in the whole area ( i ). This trend was similar for in 20-week-old mice on the bottom ( j ) and middle ( k ) sections, but the top ( l ) section did not differ significantly between the diet models. IgG1-immunopositive cells in the whole area ( m ) were significantly different between 12- and 20-week-old SCD-fed mice, and between SCD- and HFD-fed 20-week-old mice. Similarly, a significant difference in the distribution of IgG1-immunopositive cells was found in the middle ( o ) and top ( p ) sections. However, in the bottom section, a significant difference was found only between 20-week-old SCD-fed and HFD-fed mice ( n ). For the sake of comparison, we defined the central part of the villi as per a method of villi division detailed in the Quantitative analysis sub-section of the Materials and Methods. The same number of animals was used in all groups ( n = 5 per group). Results are presented as the mean ± SEM of the number of cells per μm 2 . * p

    Journal: International Journal of Molecular Sciences

    Article Title: High-Fat Diet and Age-Dependent Effects of IgA-Bearing Cell Populations in the Small Intestinal Lamina Propria in Mice

    doi: 10.3390/ijms22031165

    Figure Lengend Snippet: The number of Ig-immunopositive cells in the lamina propria of small intestinal villi. All cells in the whole lamina propria in the small intestinal villi were significantly different between 12-week and 20-week-old SCD-fed mice and between 12-week and 20-week-old HFD-fed mice ( a ). This trend was similar for all cells in the bottom ( b ) and middle ( c ) sections, but not for the top section ( d ). IgA-immunopositive cells in the whole area ( e ) were significantly different between 12- and 20-week-old SCD-fed mice and between SCD and HFD-fed 20-week-old mice. This trend was confirmed in all IgA-immunopositive cell comparisons, in the bottom ( f ), middle ( g ), and top ( h ) sections. IgM-immunopositive cells were significantly different between HFD and SCD-fed 12-week and 20-week-old mice in the whole area ( i ). This trend was similar for in 20-week-old mice on the bottom ( j ) and middle ( k ) sections, but the top ( l ) section did not differ significantly between the diet models. IgG1-immunopositive cells in the whole area ( m ) were significantly different between 12- and 20-week-old SCD-fed mice, and between SCD- and HFD-fed 20-week-old mice. Similarly, a significant difference in the distribution of IgG1-immunopositive cells was found in the middle ( o ) and top ( p ) sections. However, in the bottom section, a significant difference was found only between 20-week-old SCD-fed and HFD-fed mice ( n ). For the sake of comparison, we defined the central part of the villi as per a method of villi division detailed in the Quantitative analysis sub-section of the Materials and Methods. The same number of animals was used in all groups ( n = 5 per group). Results are presented as the mean ± SEM of the number of cells per μm 2 . * p

    Article Snippet: Then, the immunostained sections were incubated with horseradish peroxidase-conjugated donkey anti-goat (Goat IgG whole molecule, 1/400 dilution, Abcam Inc., Cambridge, MA, USA, ab6667) for IgA and -IgG1 or goat anti-rabbit IgG (Rabbit IgG whole molecule, 1/400 dilution, Abcam Inc., Cambridge, MA, USA, ab7171) for IgM, at room temperature for 1 h and finally visualized with diaminobenzidine (DAB) in a buffer solution containing hydrogen peroxide for 3 or 5 min.

    Techniques: Mouse Assay

    Comparison of the intestinal villi of standard chow diet (SCD) and high-fat-diet (HFD) fed 12-week-old mice. Paraffin-embedded tissue serial sections were obtained from each diet model. HE-staining of villi in the small intestines from SCD-fed mice showed regularly aligned simple columnar epithelium ( a , b ). HE-staining of tissues from SCD and HFD-fed mice showed red blood cells (arrowheads in b , d ). HE-staining of tissue sections from HFD-fed mice revealed vacuoles in simple columnar epithelial cells ( c , white arrows in e ). IgA-immunoreactivity was detected in the cytoplasm of epithelial cells in SCD ( f – h ) and HFD-fed mice ( i – k ), and many IgA-immunopositive cells were also observed in the lamina propria of intestinal villi (black arrows in h , k ). However, IgA-immunopositive cells in the lamina propria of intestinal villi in HFD-fed mice showed localization toward the bottom, with decreased immunopositivity compared to that in SCD-fed mice. IgM and IgG1 stainings showed common findings: Ig-immunopositive vessels (white arrowheads in m , p,s,v ) and Ig-immunopositive cells (arrows in n , q , t , w ) were observed. However, Ig-immunopositive vessels in HFD-fed mice were not immunopositive for both IgM and IgG1 (white arrowheads in p , v ). The inset also indicates immuno-controls ( f , i , l , o , r , u ).

    Journal: International Journal of Molecular Sciences

    Article Title: High-Fat Diet and Age-Dependent Effects of IgA-Bearing Cell Populations in the Small Intestinal Lamina Propria in Mice

    doi: 10.3390/ijms22031165

    Figure Lengend Snippet: Comparison of the intestinal villi of standard chow diet (SCD) and high-fat-diet (HFD) fed 12-week-old mice. Paraffin-embedded tissue serial sections were obtained from each diet model. HE-staining of villi in the small intestines from SCD-fed mice showed regularly aligned simple columnar epithelium ( a , b ). HE-staining of tissues from SCD and HFD-fed mice showed red blood cells (arrowheads in b , d ). HE-staining of tissue sections from HFD-fed mice revealed vacuoles in simple columnar epithelial cells ( c , white arrows in e ). IgA-immunoreactivity was detected in the cytoplasm of epithelial cells in SCD ( f – h ) and HFD-fed mice ( i – k ), and many IgA-immunopositive cells were also observed in the lamina propria of intestinal villi (black arrows in h , k ). However, IgA-immunopositive cells in the lamina propria of intestinal villi in HFD-fed mice showed localization toward the bottom, with decreased immunopositivity compared to that in SCD-fed mice. IgM and IgG1 stainings showed common findings: Ig-immunopositive vessels (white arrowheads in m , p,s,v ) and Ig-immunopositive cells (arrows in n , q , t , w ) were observed. However, Ig-immunopositive vessels in HFD-fed mice were not immunopositive for both IgM and IgG1 (white arrowheads in p , v ). The inset also indicates immuno-controls ( f , i , l , o , r , u ).

    Article Snippet: Then, the immunostained sections were incubated with horseradish peroxidase-conjugated donkey anti-goat (Goat IgG whole molecule, 1/400 dilution, Abcam Inc., Cambridge, MA, USA, ab6667) for IgA and -IgG1 or goat anti-rabbit IgG (Rabbit IgG whole molecule, 1/400 dilution, Abcam Inc., Cambridge, MA, USA, ab7171) for IgM, at room temperature for 1 h and finally visualized with diaminobenzidine (DAB) in a buffer solution containing hydrogen peroxide for 3 or 5 min.

    Techniques: Mouse Assay, Staining