Peroxidase Conjugated Goat Anti Mouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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1) Product Images from "A chimeric IgE that mimics IgE from patients allergic to acid-hydrolyzed wheat proteins is a novel tool for in vitro allergenicity assessment of functionalized glutens"
Article Title: A chimeric IgE that mimics IgE from patients allergic to acid-hydrolyzed wheat proteins is a novel tool for in vitro allergenicity assessment of functionalized glutens
Journal: PLoS ONE
Figure Legend Snippet: Characterization of chimeric mouse/human IgE-DG1. Chimeric mouse/human IgE-DG1 was transiently expressed in HEK293 cells, and the IgE concentrations were determined by ELISA. A. Non-reduced (NR) or reduced (R) proteins from the supernatant of transfected HEK293 cells were separated by SDS-PAGE (12%) and transferred onto nitrocellulose membrane. IgE heavy and kappa light chains were detected by peroxidase-conjugated anti-human epsilon heavy chains or by anti-human kappa light chains, respectively, prior to chemiluminescent visualization. B. The activity and specificity of chimeric IgE-DG1 and its mouse monoclonal IgG counterpart (mDG1) were compared by indirect ELISA. Native gliadin- or deamidated gliadin (D-GLIA 48) -coated ELISA plates were incubated with indicated concentrations of chimeric IgE-DG1 or mDG1.
Techniques Used: Enzyme-linked Immunosorbent Assay, Transfection, SDS Page, Activity Assay, Indirect ELISA, Incubation
2) Product Images from "Activation of Cell Cycle Arrest and Apoptosis by the Proto-Oncogene Pim-2"
Article Title: Activation of Cell Cycle Arrest and Apoptosis by the Proto-Oncogene Pim-2
Journal: PLoS ONE
Figure Legend Snippet: Western blot analysis of cytoplasmic(C) and nuclear(N) expression of PIM-2 in various cell lines. Nuclear or cytoplasmic proteins (50 µg) were separated on a 15% SDS-PAGE. Blots were reacted with anti PIM-2 antibodies as primary antibody and HRP conjugated anti rabbit IgG secondary antibody. The membranes were stripped twice and reacted once with anti RCC1 antibody as control for nuclear proteins and once with anti β-tubulin as control for cytoplasmic proteins.
Techniques Used: Western Blot, Expressing, SDS Page
3) Product Images from "Cross-Reactivity among Several Recombinant Calicivirus Virus-Like Particles (VLPs) with Monoclonal Antibodies Obtained from Mice Immunized Orally with One Type of VLP"
Article Title: Cross-Reactivity among Several Recombinant Calicivirus Virus-Like Particles (VLPs) with Monoclonal Antibodies Obtained from Mice Immunized Orally with One Type of VLP
Journal: Journal of Clinical Microbiology
Figure Legend Snippet: Western blot of polypeptides from VLPs reacting with group A MAbs. The results shown are for MAbs NV23, NV37, F8, and F120 and VLPs rSV, rNV, rSeV, rCV, rFUV, rSMA, rGV, rKAV, rNAV, rCHV, and rUEV. Proteins were boiled for 2 min and lysed with sample buffer (about 1 to 2 μg/well), and SDS-polyacrylamide gel electrophoresis (PAGE) was conducted in 10% polyacrylamide gels. The separated proteins were transferred to nitrocellulose sheets, which were incubated overnight at room temperature with the indicated MAbs (at a 1:1,000 dilution of ascites) and then incubated with HRPO-conjugated goat anti-mouse IgG, IgM, and IgA for 1 h at 37°C and treated with substrate (4-chloro-1-naphthol). Calibration kits were used for molecular weight determination (lane M); numbers on the left side indicate the apparent molecular weights (10 3 ). These MAbs reacted with all VLPs of the NLV genus whose major polypeptide had an apparent molecular weight of 58,000 but not with rSV. Oligomers or possible breakdown bands (short arrows) were seen in some VLP preparations.
Techniques Used: Western Blot, Polyacrylamide Gel Electrophoresis, Incubation, Molecular Weight
4) Product Images from "Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae"
Article Title: Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae
Journal: Nucleic Acids Research
Figure Legend Snippet: Bmt6 interacts with Nop2 in vivo . To demonstrate the interaction of Bmt6 with the preribosomal particles and its minor nuclear localization, we immunoprecipitated the total cell extract from Bmt6-TAP–tagged strain using IgG sepharose and analyzed its interaction for Nop2 by western blotting. ( A ) Western blot with PAP antibodies. ( B ) Western blot with anti-Nop2 (EnCor Biotechnology, Florida, USA) followed by anti-mouse IgG-conjugated horseradish peroxidase (Bio-Rad; 1:10 000 dilution). The samples in the different lanes of the gels are crude extract (total cell extract), flow through (unbound cell extract) and wash fraction 6 (the beads were washed six times with IPP-150 buffer) and eluate.
Techniques Used: In Vivo, Immunoprecipitation, Western Blot, Flow Cytometry
5) Product Images from "Passive Protection against Anthrax by Using a High-Affinity Antitoxin Antibody Fragment Lacking an Fc Region "
Article Title: Passive Protection against Anthrax by Using a High-Affinity Antitoxin Antibody Fragment Lacking an Fc Region
Journal: Infection and Immunity
Figure Legend Snippet: Pharmacokinetic analysis of Hartley guinea pigs injected subcutaneously with 14B7 IgG (black), M18 scAb conjugated to 20- kDa PEG (red), or M18 scAb conjugated to 40-kDa PEG (green). scAb-20-kDa PEG displayed a half-life of 23.2 h, 14B7 displayed a half-life
Techniques Used: Injection
Article Title: Generation and Characterization of Monoclonal Antibodies against a Cyclic Variant of Hepatitis C Virus E2 Epitope 412-422
Article Snippet: .. Antigen-coated wells were incubated with 0.5 μg/ml of appropriate antibodies at 37°C for 60 min, washed as described above, and then further incubated with
Article Title: Protein and modified vaccinia virus Ankara‐based influenza virus nucleoprotein vaccines are differentially immunogenic in BALB/c mice
Article Snippet: A three‐ or fivefold dilution series of the sera was prepared in blocking buffer starting at 1 : 30 and incubated on the coated plates for 1 h. Blocking buffer and anti‐NP positive serum were used as a negative or positive control, respectively. .. Plates were washed with PBST and incubated with anti‐IgG1 (Star 132P; AbD Serotec, Raleigh, NC, USA) or
Article Title: Thymic stromal lymphopoietin (TSLP) acts as a potent mucosal adjuvant for HIV-1 gp140 vaccination in mice
Article Snippet: .. Bound IgA and IgG was detected by incubation for 1 h at 37°C with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, IgE,
Article Title: A Nonhuman Primate Scrub Typhus Model: Protective Immune Responses Induced by pKarp47 DNA Vaccination in Cynomolgus Macaques
Article Snippet: The ELISA assays used a recombinant protein, 47-kDa Ag strain Karp (Kp r47b), supplied by Invitrogen (0.05 μg/well; Carlsbad, CA), or O. tsutsugamushi Karp whole-cell Ag (at 0.1 μg/well) as previously described ( , ). .. Isotyping was performed using recombinant protein Kpr47b as the Ag detected with HRP-conjugated anti-human IgG1, IgG2, IgG3, and
Article Title: Human NUMB6 Induces Epithelial-Mesenchymal Transition and Enhances Breast Cancer Cells Migration and Invasion
Article Snippet: Protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA). .. Membranes were probed for GFP (Invitrogen), and β-actin (Sigma-Aldrich), followed by