goat anti mouse igg h l cross adsorbed secondary antibody  (Thermo Fisher)


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    Name:
    Goat anti Rabbit IgG H L Highly Cross Adsorbed Secondary Antibody
    Description:
    Goat anti Rabbit IgG H L Highly Cross Adsorbed Secondary Antibody for IF ICC Flow
    Catalog Number:
    A11034
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Antibodies and Secondary Detection|Cell Analysis|Secondary Detection
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    Structured Review

    Thermo Fisher goat anti mouse igg h l cross adsorbed secondary antibody
    Goat anti Rabbit IgG H L Highly Cross Adsorbed Secondary Antibody for IF ICC Flow
    https://www.bioz.com/result/goat anti mouse igg h l cross adsorbed secondary antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg h l cross adsorbed secondary antibody - by Bioz Stars, 2021-04
    97/100 stars

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    other:

    Article Title: Common cytotoxic chemotherapeutics induce epithelial-mesenchymal transition (EMT) downstream of ER stress
    Article Snippet: Alexa Fluor 488 goat anti-rabbit IgG #A11034 and Alexa Fluor 546 goat anti-rabbit IgG #A11010 (Molecular Probes, Invitrogen detection technologies, Eugene, OR.

    Incubation:

    Article Title: Cx26 partial loss causes accelerated presbycusis by redox imbalance and dysregulation of Nfr2 pathway
    Article Snippet: 2.9.2 Immunofluorescence analyses Specimens were incubated with a blocking solution (1%BSA [Bovine Serum Albumin, Cat. No. A9647, Sigma-Aldrich], 0.5% Triton X-100 [Cat. No. T8787, Sigma-Aldrich] and 10% normal goat serum [Cat. No. S26-M, Sigma-Aldrich] in PBS 0.1 M), thereafter slices were incubated overnight at 4 °C with a solution containing anti-GSH (Cat. No. 19534, Abcam, Cambridge, MA, USA) or anti-HO-1 (Cat. No. ADI-SPA-896D Stressgen, Victoria, Canada) and Nrf2 (Cat. No. 89443, Abcam) primary antibodies diluted 1:100 in PBS. .. After washing in PBS, samples were incubated at room temperature for 2 h in labeled conjugated goat anti-rabbit (HO-1) (Alexa Fuor 488 Cat. No. A-11034, ThermoFischer, Waltham, MA, USA.) or donkey anti-mouse (GSH, Nrf2) secondary antibody (Alexa Fluor 488, Cat. No. A- 21202; or Alexa Fluor 546, IgG, Cat. No. A 10036, ThermoFischer) diluted 1:400 in 0.1 M PBS and stained with DAPI stained (1:500 in 0.1 M PBS). .. 2.10 Transfer assay for the non-metabolizable D -glucose analogue 2-NBDG 2-(N-(7-nitrobenz-2-oxa-1,3-dia-zol-4-yl)amino)-2-deoxyglucose (2-NBDG, ThermoFisher Cat. No. N13195, MW = 342.3) is a fluorescent glucose analogue that has been used to monitor glucose uptake in live cells and in the sensory epithelium of the cochlea .

    Article Title: Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging
    Article Snippet: Immunocytochemistry Cells were fixed with 4% paraformaldehyde in phosphate buffered saline (PBS), permeabilized with 100% methanol, washed with PBS, and immunostained overnight at 4°C with the following primary antibodies at 1:200 dilutions; mouse monoclonal TuJ1 (R & D systems, Minneapolis, MN, USA), rabbit anti-phosphoTDP-43 (pSer409/410) (Cosmo Bio), rabbit anti-TDP-43 C-terminus (Abcam, Cambridge, MA, USA), rabbit anti-GFP (Abcam), rabbit anti-GFAP (DAKO, Glostrup, Denmark), rabbit anti-ubiquitin (DAKO) and rabbit anti-CNP (Cell Signaling Technology, Danvers, MA, USA). .. The cells were then incubated with Alexa Fluor 488- or 633-conjugated goat anti-rabbit or anti-mouse antibodies (Thermo Fisher) at 1:400 dilutions for 45 min at room temperature, followed by incubation for 15 min with 2 μg/mL Hoechst 33342 (Thermo Fisher). ..

    Labeling:

    Article Title: Cx26 partial loss causes accelerated presbycusis by redox imbalance and dysregulation of Nfr2 pathway
    Article Snippet: 2.9.2 Immunofluorescence analyses Specimens were incubated with a blocking solution (1%BSA [Bovine Serum Albumin, Cat. No. A9647, Sigma-Aldrich], 0.5% Triton X-100 [Cat. No. T8787, Sigma-Aldrich] and 10% normal goat serum [Cat. No. S26-M, Sigma-Aldrich] in PBS 0.1 M), thereafter slices were incubated overnight at 4 °C with a solution containing anti-GSH (Cat. No. 19534, Abcam, Cambridge, MA, USA) or anti-HO-1 (Cat. No. ADI-SPA-896D Stressgen, Victoria, Canada) and Nrf2 (Cat. No. 89443, Abcam) primary antibodies diluted 1:100 in PBS. .. After washing in PBS, samples were incubated at room temperature for 2 h in labeled conjugated goat anti-rabbit (HO-1) (Alexa Fuor 488 Cat. No. A-11034, ThermoFischer, Waltham, MA, USA.) or donkey anti-mouse (GSH, Nrf2) secondary antibody (Alexa Fluor 488, Cat. No. A- 21202; or Alexa Fluor 546, IgG, Cat. No. A 10036, ThermoFischer) diluted 1:400 in 0.1 M PBS and stained with DAPI stained (1:500 in 0.1 M PBS). .. 2.10 Transfer assay for the non-metabolizable D -glucose analogue 2-NBDG 2-(N-(7-nitrobenz-2-oxa-1,3-dia-zol-4-yl)amino)-2-deoxyglucose (2-NBDG, ThermoFisher Cat. No. N13195, MW = 342.3) is a fluorescent glucose analogue that has been used to monitor glucose uptake in live cells and in the sensory epithelium of the cochlea .

    Staining:

    Article Title: Cx26 partial loss causes accelerated presbycusis by redox imbalance and dysregulation of Nfr2 pathway
    Article Snippet: 2.9.2 Immunofluorescence analyses Specimens were incubated with a blocking solution (1%BSA [Bovine Serum Albumin, Cat. No. A9647, Sigma-Aldrich], 0.5% Triton X-100 [Cat. No. T8787, Sigma-Aldrich] and 10% normal goat serum [Cat. No. S26-M, Sigma-Aldrich] in PBS 0.1 M), thereafter slices were incubated overnight at 4 °C with a solution containing anti-GSH (Cat. No. 19534, Abcam, Cambridge, MA, USA) or anti-HO-1 (Cat. No. ADI-SPA-896D Stressgen, Victoria, Canada) and Nrf2 (Cat. No. 89443, Abcam) primary antibodies diluted 1:100 in PBS. .. After washing in PBS, samples were incubated at room temperature for 2 h in labeled conjugated goat anti-rabbit (HO-1) (Alexa Fuor 488 Cat. No. A-11034, ThermoFischer, Waltham, MA, USA.) or donkey anti-mouse (GSH, Nrf2) secondary antibody (Alexa Fluor 488, Cat. No. A- 21202; or Alexa Fluor 546, IgG, Cat. No. A 10036, ThermoFischer) diluted 1:400 in 0.1 M PBS and stained with DAPI stained (1:500 in 0.1 M PBS). .. 2.10 Transfer assay for the non-metabolizable D -glucose analogue 2-NBDG 2-(N-(7-nitrobenz-2-oxa-1,3-dia-zol-4-yl)amino)-2-deoxyglucose (2-NBDG, ThermoFisher Cat. No. N13195, MW = 342.3) is a fluorescent glucose analogue that has been used to monitor glucose uptake in live cells and in the sensory epithelium of the cochlea .

    Transduction:

    Article Title: Role of lipid rafts in E-cadherin- and HGF-R/Met-mediated entry of Listeria monocytogenes into host cells
    Article Snippet: .. Reagents and antibodies Alexa Fluor 488 cholera toxin subunit B, Alexa Fluor 488–, 546–, and 647–conjugated phalloidin, Alexa Fluor 488– and 546–conjugated goat anti–rabbit and goat anti–mouse antibodies were purchased from Molecular Probes. mAbs anti–E-cadherin (clone HECD-1) was purchased from R & D systems, anti–α-catenin (clone 5) was purchased from Transduction Laboratories. ..

    Immunofluorescence:

    Article Title: Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes
    Article Snippet: .. Indirect immunofluorescence was performed using secondary antibodies conjugated with Alexa-647 anti-Mouse (Invitrogen, Cat. A-21236), Alexa-555 anti-Rat (Invitrogen, Cat. A-21434) and Alexa-488 anti-Rabbit (Invitrogen, Cat. A-11034). .. 10 mg/ml Hoechst 33342 stock solution was purchased from Life Technologies (Cat. H3570).

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  • 95
    Thermo Fisher cross adsorbed goat anti mouse igg conjugated to alexa fluor 568
    Confocal microscopy and adherence-invasion assays with pharmacologic inhibitors. Lipid raft-independent invasion of bronchial epithelial cells by NTHI. (A) Confocal microscopy. At 4 and 24 h postinoculation of H292 cells, NTHI does not colocalize with vesicles positive for the following markers of lipid rafts: caveolin-1 (shown at 24 h), flotillin-1 (shown at 24 h), and cholesterol (shown at 4 h). NTHI was labeled with anti-11P6H antibody conjugated to <t>Alexa</t> Fluor 488 (green). Caveolin-1 was labeled with anti-caveolin-1 antibody and goat anti-mouse antibody conjugated to Alexa <t>Fluor</t> 568 (red). Flotillin-1 was labeled with anti-flotillin-1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). Cholesterol was labeled with filipin (blue), and those samples were also labeled with anti-human secretory component antibody and donkey anti-goat antibody conjugated to Alexa Fluor 568 (red) to provide additional visualization of the plasma membrane. (B) Confocal microscopy. Filipin (FLP) and nystatin (NST) inhibit the internalization of fluorescently conjugated cholera toxin B subunit (white arrows), a known cargo of lipid raft-mediated endocytosis. H292 cells were pretreated with sRPMI containing inhibitor or inhibitor diluent, followed by addition of the cargo conjugate, incubation for 20 min on ice, and incubation for 2 h at 37°C. (C) Adherence-invasion assays. Filipin and nystatin do not inhibit invasion by NTHI. H292 cells were pretreated with inhibitor or inhibitor diluent for 2 h, followed by a 4-h infection conducted according to the adherence-invasion assay protocol. Data are normalized to samples in the absence of inhibitor. Error bars represent standard errors of the means of three independent experiments. A paired Student t test was used to calculate statistical significance. *, P ≤ 0.05; **, P ≤ 0.005.
    Cross Adsorbed Goat Anti Mouse Igg Conjugated To Alexa Fluor 568, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cross adsorbed goat anti mouse igg conjugated to alexa fluor 568/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cross adsorbed goat anti mouse igg conjugated to alexa fluor 568 - by Bioz Stars, 2021-04
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    97
    Thermo Fisher alexa 488 conjugated secondary anti goat antibodies
    Expression analysis of Wnt/β-catenin target genes, CD44 and EphB2, in the gastrointestinal tract of Ad Dkk1- or Ad Fc-treated adult C57BL/6 mice (12–16 weeks old). Organs were harvested 2 days after Ad Dkk1 i.v injection (10 9 pfu). ( Left ) Ad Dkk1 repression of CD44 expression in proliferative zones of all levels of the gastrointestinal epithelium. Arrowheads indicate the absence of CD44 immunoreactivity in proliferative compartments of the intestinal epithelium in Ad Dkk1 animals. * , residual CD44 staining in nonepithelial lamina propria. ( Right ) Ad Dkk1 repression of EphB2 in small intestine and colon. Repression was weaker in ascending colon and no repression was observed in stomach. EphB2 immunofluorescence was performed with <t>Alexa</t> 488 detection of EphB2 immunoreactivity (green) and Hoechst 33342 nuclear counterstain (blue). Stomach (st), duodenum (du), jejunum (je), ileum (il), cecum (ce), ascending colon (ac), and descending colon (dc) are shown.
    Alexa 488 Conjugated Secondary Anti Goat Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 488 conjugated secondary anti goat antibodies/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    97
    Thermo Fisher alexa 488 conjugated anti mouse goat antibody
    Expression of IL-6 following MyD88 inhibitor and TLR3 ligand treatment.  (A)  Fluorescent microscopy image of T47D cells, treated with TLR3 ligand (10 μg/ml) with or without MyD88 inhibitor (1 μM) following immunocytochemical staining with antibody against IL-6 and Alexa 488-tagged secondary antibody and counterstained with DAPI. Untreated indicates the cells are not treated with TLR3 ligand (magnification, 40X).  (B)  Bar graph showing the expression of IL-6 following observation through a microscope and analyses through the ImageJ software for all the experiment groups.  (C,D)  Expression of IL-6 in the cell culture supernatant as measured through ELISA. The results are presented as mean ± SD ( p
    Alexa 488 Conjugated Anti Mouse Goat Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 488 conjugated anti mouse goat antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa 488 conjugated anti mouse goat antibody - by Bioz Stars, 2021-04
    97/100 stars
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    Image Search Results


    Confocal microscopy and adherence-invasion assays with pharmacologic inhibitors. Lipid raft-independent invasion of bronchial epithelial cells by NTHI. (A) Confocal microscopy. At 4 and 24 h postinoculation of H292 cells, NTHI does not colocalize with vesicles positive for the following markers of lipid rafts: caveolin-1 (shown at 24 h), flotillin-1 (shown at 24 h), and cholesterol (shown at 4 h). NTHI was labeled with anti-11P6H antibody conjugated to Alexa Fluor 488 (green). Caveolin-1 was labeled with anti-caveolin-1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). Flotillin-1 was labeled with anti-flotillin-1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). Cholesterol was labeled with filipin (blue), and those samples were also labeled with anti-human secretory component antibody and donkey anti-goat antibody conjugated to Alexa Fluor 568 (red) to provide additional visualization of the plasma membrane. (B) Confocal microscopy. Filipin (FLP) and nystatin (NST) inhibit the internalization of fluorescently conjugated cholera toxin B subunit (white arrows), a known cargo of lipid raft-mediated endocytosis. H292 cells were pretreated with sRPMI containing inhibitor or inhibitor diluent, followed by addition of the cargo conjugate, incubation for 20 min on ice, and incubation for 2 h at 37°C. (C) Adherence-invasion assays. Filipin and nystatin do not inhibit invasion by NTHI. H292 cells were pretreated with inhibitor or inhibitor diluent for 2 h, followed by a 4-h infection conducted according to the adherence-invasion assay protocol. Data are normalized to samples in the absence of inhibitor. Error bars represent standard errors of the means of three independent experiments. A paired Student t test was used to calculate statistical significance. *, P ≤ 0.05; **, P ≤ 0.005.

    Journal: Infection and Immunity

    Article Title: Internalization and Trafficking of Nontypeable Haemophilus influenzae in Human Respiratory Epithelial Cells and Roles of IgA1 Proteases for Optimal Invasion and Persistence

    doi: 10.1128/IAI.00864-13

    Figure Lengend Snippet: Confocal microscopy and adherence-invasion assays with pharmacologic inhibitors. Lipid raft-independent invasion of bronchial epithelial cells by NTHI. (A) Confocal microscopy. At 4 and 24 h postinoculation of H292 cells, NTHI does not colocalize with vesicles positive for the following markers of lipid rafts: caveolin-1 (shown at 24 h), flotillin-1 (shown at 24 h), and cholesterol (shown at 4 h). NTHI was labeled with anti-11P6H antibody conjugated to Alexa Fluor 488 (green). Caveolin-1 was labeled with anti-caveolin-1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). Flotillin-1 was labeled with anti-flotillin-1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). Cholesterol was labeled with filipin (blue), and those samples were also labeled with anti-human secretory component antibody and donkey anti-goat antibody conjugated to Alexa Fluor 568 (red) to provide additional visualization of the plasma membrane. (B) Confocal microscopy. Filipin (FLP) and nystatin (NST) inhibit the internalization of fluorescently conjugated cholera toxin B subunit (white arrows), a known cargo of lipid raft-mediated endocytosis. H292 cells were pretreated with sRPMI containing inhibitor or inhibitor diluent, followed by addition of the cargo conjugate, incubation for 20 min on ice, and incubation for 2 h at 37°C. (C) Adherence-invasion assays. Filipin and nystatin do not inhibit invasion by NTHI. H292 cells were pretreated with inhibitor or inhibitor diluent for 2 h, followed by a 4-h infection conducted according to the adherence-invasion assay protocol. Data are normalized to samples in the absence of inhibitor. Error bars represent standard errors of the means of three independent experiments. A paired Student t test was used to calculate statistical significance. *, P ≤ 0.05; **, P ≤ 0.005.

    Article Snippet: Host cell components were visualized using filipin III fluorescent dye (final concentration of 333 μg/ml; Cayman Chemical), primary monoclonal antibodies including mouse anti-caveolin-1 antibody (1:50; BD), mouse anti-early endosomal antigen 1 (EEA-1) antibody (1:200; BD), mouse anti-flotillin-1 antibody (1:20; BD), mouse anti-human CD107a antibody (LAMP1) (1:200; BD), and goat anti-human secretory component antibody (1:200; Sigma), and secondary antibodies including highly cross-adsorbed goat anti-mouse IgG conjugated to Alexa Fluor 568 (Life Technologies, 1:200) and donkey anti-goat IgG conjugated to Alexa Fluor 568 (Life Technologies, 1:200).

    Techniques: Confocal Microscopy, Labeling, Incubation, Infection, Invasion Assay

    Confocal microscopy and survival assay with pharmacologic inhibitor of lysosome acidification. NTHI traffics to early endosomes and lysosomes and is killed in lysosomes. IgA1 proteases are required for optimal survival in lysosomes. (A) Confocal microscopy. At 4 and 24 h postinoculation of H292 cells, NTHI is found within vesicles positive for EEA1 (shown at 4 h) and within vesicles positive for LAMP1 (shown at 24 h). NTHI was labeled using anti-11P6H antibody conjugated to Alexa Fluor 488 (green). EEA1 was labeled using anti-EEA1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). LAMP1 was labeled using anti-human LAMP1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). (B) Survival assay in the presence and absence of concanamycin A (CMA). H292 cells were infected for 16 h, treated with gentamicin for 1 h, and treated with concanamycin or diluent for 3 h. Survival data are normalized to samples treated with diluent. Error bars represent standard errors of the means of three independent experiments. A paired Student t test was used to calculate statistical significance. *, P ≤ 0.05; **, P ≤ 0.005.

    Journal: Infection and Immunity

    Article Title: Internalization and Trafficking of Nontypeable Haemophilus influenzae in Human Respiratory Epithelial Cells and Roles of IgA1 Proteases for Optimal Invasion and Persistence

    doi: 10.1128/IAI.00864-13

    Figure Lengend Snippet: Confocal microscopy and survival assay with pharmacologic inhibitor of lysosome acidification. NTHI traffics to early endosomes and lysosomes and is killed in lysosomes. IgA1 proteases are required for optimal survival in lysosomes. (A) Confocal microscopy. At 4 and 24 h postinoculation of H292 cells, NTHI is found within vesicles positive for EEA1 (shown at 4 h) and within vesicles positive for LAMP1 (shown at 24 h). NTHI was labeled using anti-11P6H antibody conjugated to Alexa Fluor 488 (green). EEA1 was labeled using anti-EEA1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). LAMP1 was labeled using anti-human LAMP1 antibody and goat anti-mouse antibody conjugated to Alexa Fluor 568 (red). (B) Survival assay in the presence and absence of concanamycin A (CMA). H292 cells were infected for 16 h, treated with gentamicin for 1 h, and treated with concanamycin or diluent for 3 h. Survival data are normalized to samples treated with diluent. Error bars represent standard errors of the means of three independent experiments. A paired Student t test was used to calculate statistical significance. *, P ≤ 0.05; **, P ≤ 0.005.

    Article Snippet: Host cell components were visualized using filipin III fluorescent dye (final concentration of 333 μg/ml; Cayman Chemical), primary monoclonal antibodies including mouse anti-caveolin-1 antibody (1:50; BD), mouse anti-early endosomal antigen 1 (EEA-1) antibody (1:200; BD), mouse anti-flotillin-1 antibody (1:20; BD), mouse anti-human CD107a antibody (LAMP1) (1:200; BD), and goat anti-human secretory component antibody (1:200; Sigma), and secondary antibodies including highly cross-adsorbed goat anti-mouse IgG conjugated to Alexa Fluor 568 (Life Technologies, 1:200) and donkey anti-goat IgG conjugated to Alexa Fluor 568 (Life Technologies, 1:200).

    Techniques: Confocal Microscopy, Clonogenic Cell Survival Assay, Labeling, Infection

    Expression analysis of Wnt/β-catenin target genes, CD44 and EphB2, in the gastrointestinal tract of Ad Dkk1- or Ad Fc-treated adult C57BL/6 mice (12–16 weeks old). Organs were harvested 2 days after Ad Dkk1 i.v injection (10 9 pfu). ( Left ) Ad Dkk1 repression of CD44 expression in proliferative zones of all levels of the gastrointestinal epithelium. Arrowheads indicate the absence of CD44 immunoreactivity in proliferative compartments of the intestinal epithelium in Ad Dkk1 animals. * , residual CD44 staining in nonepithelial lamina propria. ( Right ) Ad Dkk1 repression of EphB2 in small intestine and colon. Repression was weaker in ascending colon and no repression was observed in stomach. EphB2 immunofluorescence was performed with Alexa 488 detection of EphB2 immunoreactivity (green) and Hoechst 33342 nuclear counterstain (blue). Stomach (st), duodenum (du), jejunum (je), ileum (il), cecum (ce), ascending colon (ac), and descending colon (dc) are shown.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Essential requirement for Wnt signaling in proliferation of adult small intestine and colon revealed by adenoviral expression of Dickkopf-1

    doi: 10.1073/pnas.2536800100

    Figure Lengend Snippet: Expression analysis of Wnt/β-catenin target genes, CD44 and EphB2, in the gastrointestinal tract of Ad Dkk1- or Ad Fc-treated adult C57BL/6 mice (12–16 weeks old). Organs were harvested 2 days after Ad Dkk1 i.v injection (10 9 pfu). ( Left ) Ad Dkk1 repression of CD44 expression in proliferative zones of all levels of the gastrointestinal epithelium. Arrowheads indicate the absence of CD44 immunoreactivity in proliferative compartments of the intestinal epithelium in Ad Dkk1 animals. * , residual CD44 staining in nonepithelial lamina propria. ( Right ) Ad Dkk1 repression of EphB2 in small intestine and colon. Repression was weaker in ascending colon and no repression was observed in stomach. EphB2 immunofluorescence was performed with Alexa 488 detection of EphB2 immunoreactivity (green) and Hoechst 33342 nuclear counterstain (blue). Stomach (st), duodenum (du), jejunum (je), ileum (il), cecum (ce), ascending colon (ac), and descending colon (dc) are shown.

    Article Snippet: Stainings were visualized with Alexa 488-conjugated secondary anti-goat antibodies (Molecular Probes) and nuclei were counterstained with Hoechst 33342 (Molecular Probes).

    Techniques: Expressing, Mouse Assay, Injection, Staining, Immunofluorescence

    Expression of IL-6 following MyD88 inhibitor and TLR3 ligand treatment.  (A)  Fluorescent microscopy image of T47D cells, treated with TLR3 ligand (10 μg/ml) with or without MyD88 inhibitor (1 μM) following immunocytochemical staining with antibody against IL-6 and Alexa 488-tagged secondary antibody and counterstained with DAPI. Untreated indicates the cells are not treated with TLR3 ligand (magnification, 40X).  (B)  Bar graph showing the expression of IL-6 following observation through a microscope and analyses through the ImageJ software for all the experiment groups.  (C,D)  Expression of IL-6 in the cell culture supernatant as measured through ELISA. The results are presented as mean ± SD ( p

    Journal: Frontiers in Oncology

    Article Title: Myeloid Differentiation Primary Response 88–Cyclin D1 Signaling in Breast Cancer Cells Regulates Toll-Like Receptor 3-Mediated Cell Proliferation

    doi: 10.3389/fonc.2020.01780

    Figure Lengend Snippet: Expression of IL-6 following MyD88 inhibitor and TLR3 ligand treatment. (A) Fluorescent microscopy image of T47D cells, treated with TLR3 ligand (10 μg/ml) with or without MyD88 inhibitor (1 μM) following immunocytochemical staining with antibody against IL-6 and Alexa 488-tagged secondary antibody and counterstained with DAPI. Untreated indicates the cells are not treated with TLR3 ligand (magnification, 40X). (B) Bar graph showing the expression of IL-6 following observation through a microscope and analyses through the ImageJ software for all the experiment groups. (C,D) Expression of IL-6 in the cell culture supernatant as measured through ELISA. The results are presented as mean ± SD ( p

    Article Snippet: For IL-6 expression, cells were fixed, permeabilized, and incubated with primary IL-6 antibody (Invitrogen-AMC0862) and Alexa 488-conjugated anti-mouse goat antibody (Invitrogen-A11001) and mounted with Vecta Shield-DAPI to counterstain the nuclei and were observed under fluorescence microscope (Leica DMI 6000B).

    Techniques: Expressing, Microscopy, Staining, Software, Cell Culture, Enzyme-linked Immunosorbent Assay

    Validation of external S. aureus labeling. Adherent GM-MФs were either treated with cytochalasin D (to block uptake) or control prior to and during incubation with GFP- S. aureus RN6390 and incubated with lysostaphin (to degrade external S. aureus , eliminating external bacteria) or control following incubation with S. aureus . External S. aureus were labeled with an IgG 3 monoclonal mouse anti- S. aureus primary antibody and Texas-RedX-conjugated goat anti-mouse secondary antibody. (A) No treatment. (B) Treatment with cytochalasin D. (C) Treatment with lysostaphin. (D) Treatment with both cytochalasin D and lysostaphin. (Original magnification, 200X).

    Journal: PLoS ONE

    Article Title: Heterogeneity in Macrophage Phagocytosis of Staphylococcus aureus Strains: High-Throughput Scanning Cytometry-Based Analysis

    doi: 10.1371/journal.pone.0006209

    Figure Lengend Snippet: Validation of external S. aureus labeling. Adherent GM-MФs were either treated with cytochalasin D (to block uptake) or control prior to and during incubation with GFP- S. aureus RN6390 and incubated with lysostaphin (to degrade external S. aureus , eliminating external bacteria) or control following incubation with S. aureus . External S. aureus were labeled with an IgG 3 monoclonal mouse anti- S. aureus primary antibody and Texas-RedX-conjugated goat anti-mouse secondary antibody. (A) No treatment. (B) Treatment with cytochalasin D. (C) Treatment with lysostaphin. (D) Treatment with both cytochalasin D and lysostaphin. (Original magnification, 200X).

    Article Snippet: To achieve red fluorescent labeling of external bacteria cells were incubated for 15 minutes with PBS/4% FBS (blocking buffer), incubated for 40 minutes with 10 µg/ml mouse monoclonal IgG3 anti-S. aureus antibody in blocking buffer, washed three times with blocking buffer, incubated for 20 minutes with 40 µg/ml Texas-RedX-conjugated goat anti-mouse IgG (Life Technologies) in blocking buffer, and washed twice with blocking buffer.

    Techniques: Labeling, Blocking Assay, Incubation

    Scanning cytometry fluorescence imaging with GFP-S. aureus RN6390. Adherent GM-MФs were incubated with unopsonized GFP- S. aureus RN6390. External S. aureus were labeled with an IgG 3 monoclonal mouse anti- S. aureus primary antibody and Texas-RedX-conjugated goat anti-mouse secondary antibody. Collapsed confocal stack images were acquired by scanning cytometry. (A) CellTracker Blue and Hoechst channel (cells). (B) GFP channel (all bacteria). (C) Texas Red channel (external bacteria). (D) Composite image. (Original magnification, 200X).

    Journal: PLoS ONE

    Article Title: Heterogeneity in Macrophage Phagocytosis of Staphylococcus aureus Strains: High-Throughput Scanning Cytometry-Based Analysis

    doi: 10.1371/journal.pone.0006209

    Figure Lengend Snippet: Scanning cytometry fluorescence imaging with GFP-S. aureus RN6390. Adherent GM-MФs were incubated with unopsonized GFP- S. aureus RN6390. External S. aureus were labeled with an IgG 3 monoclonal mouse anti- S. aureus primary antibody and Texas-RedX-conjugated goat anti-mouse secondary antibody. Collapsed confocal stack images were acquired by scanning cytometry. (A) CellTracker Blue and Hoechst channel (cells). (B) GFP channel (all bacteria). (C) Texas Red channel (external bacteria). (D) Composite image. (Original magnification, 200X).

    Article Snippet: To achieve red fluorescent labeling of external bacteria cells were incubated for 15 minutes with PBS/4% FBS (blocking buffer), incubated for 40 minutes with 10 µg/ml mouse monoclonal IgG3 anti-S. aureus antibody in blocking buffer, washed three times with blocking buffer, incubated for 20 minutes with 40 µg/ml Texas-RedX-conjugated goat anti-mouse IgG (Life Technologies) in blocking buffer, and washed twice with blocking buffer.

    Techniques: Cytometry, Fluorescence, Imaging, Incubation, Labeling