goat anti mouse hrp conjugated polyclonal antibodies  (Millipore)

 
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    Name:
    Anti Mouse IgG whole molecule Peroxidase antibody
    Description:
    Immunoglobulin G IgG is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases Mouse IgGs have four distinct isotypes namely IgG1 IgG2a IgG2b and IgG3 IgG1 regulates complement fixation in mice Anti Mouse IgG whole molecule Peroxidase antibody is specific for mouse IgGs
    Catalog Number:
    A4416
    Price:
    None
    Applications:
    Western blot analysis of transfected Hela cell lysates was performed using HRP conjugated goat anti-mouse IgG as the secondary at a dilution of 1:4000.
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    Structured Review

    Millipore goat anti mouse hrp conjugated polyclonal antibodies
    Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B <t>polyclonal</t> (Metabiologics) was used in conjunction with an <t>HRP-conjugated,</t> goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).
    Immunoglobulin G IgG is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases Mouse IgGs have four distinct isotypes namely IgG1 IgG2a IgG2b and IgG3 IgG1 regulates complement fixation in mice Anti Mouse IgG whole molecule Peroxidase antibody is specific for mouse IgGs
    https://www.bioz.com/result/goat anti mouse hrp conjugated polyclonal antibodies/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse hrp conjugated polyclonal antibodies - by Bioz Stars, 2021-05
    99/100 stars

    Images

    1) Product Images from "Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and Its Application to Toxin Detection in Milk"

    Article Title: Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and Its Application to Toxin Detection in Milk

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0011047

    Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).
    Figure Legend Snippet: Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).

    Techniques Used: Sandwich ELISA, Quantitation Assay

    2) Product Images from "Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and Its Application to Toxin Detection in Milk"

    Article Title: Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and Its Application to Toxin Detection in Milk

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0011047

    Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).
    Figure Legend Snippet: Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).

    Techniques Used: Sandwich ELISA, Quantitation Assay

    Related Articles

    Incubation:

    Article Title: AMPA receptor-mTORC1 signaling activation is required for neuroplastic effects of LY341495 in rat hippocampal neurons
    Article Snippet: Immunoblotting was performed with one of the primary antibodies (anti-phospho-mTORC1 [Ser2448, #2971], anti-mTORC1 [#2972], anti-phosho-4E-BP-1 [Thr37/46, #2855], anti-4E-BP-1 [#9452], anti-phospho-p70S6K [Thr389, #9205], and anti-p70S6K [#9202], anti-phospho-p44/42 MAPK (ERK1/2) [Thr202/Tyr204, #9101], anti-p44/42 MAPK (ERK1/2) [#4695] [1:1000; Cell Signaling]; anti-PSD-95 [1:1000, AB9634; Millipore]; anti-GluA1 [1:1000, ab109450; Abcam]; anti-BDNF [1:1000; sc-546, Santa Cruz]; anti-α-tubulin [1:2000; T9026, Sigma]) in Tris-buffered saline with Tween-20 (TBS-T) at 4 °C overnight, and then the membranes were washed three times in TBS-T for 10 min. .. These were incubated for 1 h in TBS-T containing horseradish peroxidase-conjugated secondary antibody (goat-anti-rabbit IgG [1:2000, sc-2004; Santa Cruz] for anti-phospho-mTORC1, anti-mTORC1, anti-phospho-4E-BP-1, anti-4E-BP-1, anti-phospho-p70S6K, anti-p70S6K, anti-PSD-95, anti-GluA1, or anti-BDNF; anti-mouse IgG [1:10000, A4416; Sigma] for anti-α-tubulin) at room temperature. .. Western blotting analyses were repeated twice per sample for each of the two independent cultures.

    Article Title: Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and Its Application to Toxin Detection in Milk
    Article Snippet: Hybridoma supernatant, diluted 1∶10 in NFDM-TBST, was added to each well and incubated for 1 h at 37°C. .. The plate was washed, then a 1 µg/mL solution of goat anti-mouse HRP-conjugated polyclonal antibodies #A4416 (Sigma-Aldrich) was added and the plates were incubated at 37°C for 1 h. Plates were again washed three times, then K-Blue substrate (Neogen Corporation, Lexington, KY) was added (100 µL per well) and incubated with agitation for 5 min at room temperature. .. Stop solution (Neogen) was added (100 µL per well), and absorbance at 650 nm was measured using a VersaMax microplate reader (Molecular Devices, Sunnyvale CA).

    Affinity Purification:

    Article Title: Hepatocyte Nuclear Factor 4α Contributes to Thyroid Hormone Homeostasis by Cooperatively Regulating the Type 1 Iodothyronine Deiodinase Gene with GATA4 and Krüppel-Like Transcription Factor 9
    Article Snippet: Goat polyclonal anti-human GATA4 antibody (directed against aa 27 to 211 of human GATA) (AF2606) was from R & D Systems. .. Monoclonal anti-FLAG M2 (F3165), peroxidase-conjugated anti-FLAG M2 (A8592), mouse monoclonal anti-β-actin (A1978), and affinity-purified goat anti-mouse IgG (A4416) were from Sigma. .. Monoclonal antinucleoporin antibody (610497) was from BD- Biosciences.

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  • 99
    Millipore goat anti mouse hrp conjugated polyclonal antibodies
    Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B <t>polyclonal</t> (Metabiologics) was used in conjunction with an <t>HRP-conjugated,</t> goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).
    Goat Anti Mouse Hrp Conjugated Polyclonal Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse hrp conjugated polyclonal antibodies/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse hrp conjugated polyclonal antibodies - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    97
    Millipore polyclonal goat anti mouse igg horseradish peroxidase conjugate
    Modified <t>IgG</t> fusions have improved expression and solubility (A) ELISA and gel quantification of IgG fusion construct expression. Clarified protein extracts (“soluble” fraction) from leaf spots agroinfiltrated with each IgG fusion construct were analyzed by either ELISA, or SDS-PAGE followed by gel image quantification. For ZE3 ELISA, plates were coated with <t>polyclonal</t> mouse anti-ZE3, incubated with serial dilutions of extracts from each IgG fusion using purified HLZ as a standard, and probed with goat anti-human IgG-HRP. For IgG ELISA, plates were coated with serial dilutions of extracts or human IgG standard and probed with goat anti-human IgG-HRP. For gel quantification, ImageJ software was used to compare the IgG fusion band intensity visualized on stain-free polyacrylamide gels using purified 6D8 antibody as standard. Columns represent means ± standard error from three independently infiltrated leaf samples. (B) Clarified leaf extracts were separated by reducing SDS-PAGE and a representative gel image is shown. The band position corresponding to each respective heavy chain/ZE3 fusion is indicated “ZH/HZ.” The small shift in size in HLZe and HLZd is due to epitope tag truncation. The “-” indicates ZE3-Fc and Fc fragments. The large subunit of Rubisco “RbcL.”
    Polyclonal Goat Anti Mouse Igg Horseradish Peroxidase Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal goat anti mouse igg horseradish peroxidase conjugate/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal goat anti mouse igg horseradish peroxidase conjugate - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    99
    Millipore hrp conjugated goat anti human polyclonal polyvalent immunoglobulins
    The enzyme-labeled LD5 exhibits enhanced binding activities for IgM and IgA. The binding activities of horseradish peroxidase <t>(HRP)-labeled</t> LD5 (HRP-LD5) or HRP-conjugated goat anti-human <t>polyclonal</t> antibodies (HRP-goat anti-human PcAb) to coated hIgM (A), hIgG (B) or hIgA (C) were examined by ELISA. The coating buffer was used as solvent control.
    Hrp Conjugated Goat Anti Human Polyclonal Polyvalent Immunoglobulins, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hrp conjugated goat anti human polyclonal polyvalent immunoglobulins/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hrp conjugated goat anti human polyclonal polyvalent immunoglobulins - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).

    Journal: PLoS ONE

    Article Title: Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and Its Application to Toxin Detection in Milk

    doi: 10.1371/journal.pone.0011047

    Figure Lengend Snippet: Sandwich ELISA for detection of BoNT/B. MAb MCS6-27 was used as capture antibody, rabbit anti-BoNT/B polyclonal (Metabiologics) was used in conjunction with an HRP-conjugated, goat anti-rabbit polyclonal antibody as detector. Data are presented ±1 standard error, n = 3. Horizontal dashed lines represent the limit of detection (L.O.D., zero toxin plus 3 standard deviations) and the limit of quantitation (L.O.Q., zero toxin plus 5 standard deviations).

    Article Snippet: The plate was washed, then a 1 µg/mL solution of goat anti-mouse HRP-conjugated polyclonal antibodies #A4416 (Sigma-Aldrich) was added and the plates were incubated at 37°C for 1 h. Plates were again washed three times, then K-Blue substrate (Neogen Corporation, Lexington, KY) was added (100 µL per well) and incubated with agitation for 5 min at room temperature.

    Techniques: Sandwich ELISA, Quantitation Assay

    Modified IgG fusions have improved expression and solubility (A) ELISA and gel quantification of IgG fusion construct expression. Clarified protein extracts (“soluble” fraction) from leaf spots agroinfiltrated with each IgG fusion construct were analyzed by either ELISA, or SDS-PAGE followed by gel image quantification. For ZE3 ELISA, plates were coated with polyclonal mouse anti-ZE3, incubated with serial dilutions of extracts from each IgG fusion using purified HLZ as a standard, and probed with goat anti-human IgG-HRP. For IgG ELISA, plates were coated with serial dilutions of extracts or human IgG standard and probed with goat anti-human IgG-HRP. For gel quantification, ImageJ software was used to compare the IgG fusion band intensity visualized on stain-free polyacrylamide gels using purified 6D8 antibody as standard. Columns represent means ± standard error from three independently infiltrated leaf samples. (B) Clarified leaf extracts were separated by reducing SDS-PAGE and a representative gel image is shown. The band position corresponding to each respective heavy chain/ZE3 fusion is indicated “ZH/HZ.” The small shift in size in HLZe and HLZd is due to epitope tag truncation. The “-” indicates ZE3-Fc and Fc fragments. The large subunit of Rubisco “RbcL.”

    Journal: bioRxiv

    Article Title: A highly expressing, soluble, and stable plant-made IgG fusion carrying Zika virus envelope domain III elicits potent immunogenic responses in mice without adjuvant

    doi: 10.1101/2020.07.13.199703

    Figure Lengend Snippet: Modified IgG fusions have improved expression and solubility (A) ELISA and gel quantification of IgG fusion construct expression. Clarified protein extracts (“soluble” fraction) from leaf spots agroinfiltrated with each IgG fusion construct were analyzed by either ELISA, or SDS-PAGE followed by gel image quantification. For ZE3 ELISA, plates were coated with polyclonal mouse anti-ZE3, incubated with serial dilutions of extracts from each IgG fusion using purified HLZ as a standard, and probed with goat anti-human IgG-HRP. For IgG ELISA, plates were coated with serial dilutions of extracts or human IgG standard and probed with goat anti-human IgG-HRP. For gel quantification, ImageJ software was used to compare the IgG fusion band intensity visualized on stain-free polyacrylamide gels using purified 6D8 antibody as standard. Columns represent means ± standard error from three independently infiltrated leaf samples. (B) Clarified leaf extracts were separated by reducing SDS-PAGE and a representative gel image is shown. The band position corresponding to each respective heavy chain/ZE3 fusion is indicated “ZH/HZ.” The small shift in size in HLZe and HLZd is due to epitope tag truncation. The “-” indicates ZE3-Fc and Fc fragments. The large subunit of Rubisco “RbcL.”

    Article Snippet: Mouse antibodies were detected by incubation with polyclonal goat anti-mouse IgG-horseradish peroxidase conjugate (Sigma-Aldrich, St. Louis, MO, USA).

    Techniques: Modification, Expressing, Solubility, Enzyme-linked Immunosorbent Assay, Construct, SDS Page, Incubation, Purification, Software, Staining

    Various IgG fusion strategies enhance C1q binding (A) Schematic representation of IgG fusion constructs used in this study. Fusion constructs are built on the mAb 6D8 human IgG1 backbone with the shown modifications. ZE3; the Zika envelope domain III containing amino acids K301 to T406; e, an epitope tag containing the “VYKLDISEA” 6D8 binding motif for RIC formation; e with lightning bolt, an epitope tag truncated to include only “YKLDIS” to reduce RIC formation; VH, the variable heavy domain from 6D8 which participates in binding the epitope tag; VL, the variably light domain from 6D8 which participates in binding the epitope tag; H, the heavy chain constant CH1 domain from 6D8; L, the light chain constant domain from 6D8; Fc, the heavy chain constant CH2 or CH3 domains from 6D8; Fc with lightning bolt, same as Fc but with E345R, E430G, and S440Y mutations to induce hexamer formation. (B) C1q binding ELISA of purified IgG fusion constructs. ELISA plates were coated with 10 μg/ml human C1q and incubated with 10 μg/ml each molecule, using 6D8 with no fusion as a negative control. Constructs were detected using polyclonal goat anti-human IgG-HRP. Mean OD 450 values from three samples are shown ± standard error with one star (*) indicating p

    Journal: bioRxiv

    Article Title: A highly expressing, soluble, and stable plant-made IgG fusion carrying Zika virus envelope domain III elicits potent immunogenic responses in mice without adjuvant

    doi: 10.1101/2020.07.13.199703

    Figure Lengend Snippet: Various IgG fusion strategies enhance C1q binding (A) Schematic representation of IgG fusion constructs used in this study. Fusion constructs are built on the mAb 6D8 human IgG1 backbone with the shown modifications. ZE3; the Zika envelope domain III containing amino acids K301 to T406; e, an epitope tag containing the “VYKLDISEA” 6D8 binding motif for RIC formation; e with lightning bolt, an epitope tag truncated to include only “YKLDIS” to reduce RIC formation; VH, the variable heavy domain from 6D8 which participates in binding the epitope tag; VL, the variably light domain from 6D8 which participates in binding the epitope tag; H, the heavy chain constant CH1 domain from 6D8; L, the light chain constant domain from 6D8; Fc, the heavy chain constant CH2 or CH3 domains from 6D8; Fc with lightning bolt, same as Fc but with E345R, E430G, and S440Y mutations to induce hexamer formation. (B) C1q binding ELISA of purified IgG fusion constructs. ELISA plates were coated with 10 μg/ml human C1q and incubated with 10 μg/ml each molecule, using 6D8 with no fusion as a negative control. Constructs were detected using polyclonal goat anti-human IgG-HRP. Mean OD 450 values from three samples are shown ± standard error with one star (*) indicating p

    Article Snippet: Mouse antibodies were detected by incubation with polyclonal goat anti-mouse IgG-horseradish peroxidase conjugate (Sigma-Aldrich, St. Louis, MO, USA).

    Techniques: Binding Assay, Construct, Enzyme-linked Immunosorbent Assay, Purification, Incubation, Negative Control

    The enzyme-labeled LD5 exhibits enhanced binding activities for IgM and IgA. The binding activities of horseradish peroxidase (HRP)-labeled LD5 (HRP-LD5) or HRP-conjugated goat anti-human polyclonal antibodies (HRP-goat anti-human PcAb) to coated hIgM (A), hIgG (B) or hIgA (C) were examined by ELISA. The coating buffer was used as solvent control.

    Journal: PLoS ONE

    Article Title: Novel Evolved Immunoglobulin (Ig)-Binding Molecules Enhance the Detection of IgM against Hepatitis C Virus

    doi: 10.1371/journal.pone.0018477

    Figure Lengend Snippet: The enzyme-labeled LD5 exhibits enhanced binding activities for IgM and IgA. The binding activities of horseradish peroxidase (HRP)-labeled LD5 (HRP-LD5) or HRP-conjugated goat anti-human polyclonal antibodies (HRP-goat anti-human PcAb) to coated hIgM (A), hIgG (B) or hIgA (C) were examined by ELISA. The coating buffer was used as solvent control.

    Article Snippet: Human IgG (hIgG), human IgM (hIgM), human IgA (hIgA), goat anti-human IgG, horseradish peroxidase (HRP), HRP-conjugated streptavidin, HRP-conjugated goat anti-human polyclonal polyvalent immunoglobulins (G, A, M) (HRP-goat anti-human PcAb), HRP-conjugated anti-hIgG and HRP-conjugated anti-hIgM were obtained from Sigma (St. Louis, MO, USA). hIgG, hIgM and hIgA antibodies were biotinylated using biotinyl-N-hydroxy-succinimide (Pierce, Rockford, IL, USA) in our lab (data not shown). β-mercaptoethanol (BME) was from Janssen Chimica (Geel, Belgium).

    Techniques: Labeling, Binding Assay, Enzyme-linked Immunosorbent Assay