goat anti mouse horseradish peroxidase labeled secondary antibodies  (Abcam)

 
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    Name:
    Goat Anti Mouse IgG IgM IgA H L HRP preadsorbed
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    Catalog Number:
    AB102466
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    Structured Review

    Abcam goat anti mouse horseradish peroxidase labeled secondary antibodies
    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using <t>mouse</t> <t>anti-hITF</t> antibodies and <t>goat</t> anti-mouse <t>TRITC-labeled</t> <t>secondary</t> antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    https://www.bioz.com/result/goat anti mouse horseradish peroxidase labeled secondary antibodies/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse horseradish peroxidase labeled secondary antibodies - by Bioz Stars, 2021-05
    95/100 stars

    Images

    1) Product Images from "Recombinant Adenovirus-Mediated Intestinal Trefoil Factor Gene Therapy for Burn-Induced Intestinal Mucosal Injury"

    Article Title: Recombinant Adenovirus-Mediated Intestinal Trefoil Factor Gene Therapy for Burn-Induced Intestinal Mucosal Injury

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0062429

    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.
    Figure Legend Snippet: Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Techniques Used: Infection, Labeling, Staining

    Related Articles

    Incubation:

    Article Title: Rv0613c/MSMEG_1285 Interacts with HBHA and Mediates Its Proper Cell-Surface Exposure in Mycobacteria
    Article Snippet: The membrane was saturated with Tris-Buffered Saline (pH 7.5, TBS) containing 0.05% Tween 80 and 5% milk and probed overnight at 4 °C with anti-HBHA monoclonal antibody VF2 or D2 diluted 1:100 in TBS-Tween-3% milk or sera from Rv0613c-immunized mice diluted 1:100 in TBS-Tween-3% milk or anti-Hsp65 monoclonal antibody (Abcam, Cambridge, UK) diluted 1:1000 in TBS-Tween-3% milk. .. Finally, the membrane was incubated for 1 h at room temperature with goat anti-mouse horseradish peroxidase-conjugated secondary antibodies (Abcam), diluted 1:5000 in TBS-Tween-3% milk. .. Detection was performed using the Amersham ECL Prime Western-Blotting Detection Reagent (Fisher Scientific, Illkirch, France) and chemiluminescence was detected using the Amersham Imager 600 (GE Healthcare, Uppsala, Sweden).

    Article Title: MicroRNA-21 promotes the proliferation, migration and invasion of non-small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway
    Article Snippet: Then, the proteins were electro-transferred to polyvinylidene difluoride (PVDF) membranes on ice (250 mA, 1 h) before being blocked with 50 g/l skimmed milk at room temperature for 1 h. Afterwards, rabbit anti-human polyclonal ULK1 (1:1,000), LC3B (1:800), AMPKα (1:1,000), p-AMPK (1:800) and p62 (1:800) primary antibodies and mouse anti-human GAPDH (1:400) primary antibody (Abcam, Cambridge, UK) were added onto the membranes before incubation at 4°C overnight. .. Then, the membrane was extensively washed with phosphate-buffered saline with Tween 20 (PBST) for 5 times of 5 min, and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:4,000; Abcam) at room temperature for 1 h. Subsequently, the membrane was washed with PBST for 5 times of 5 min before the membrane was developed with enhanced chemiluminescence detection kit (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for imaging. .. We used Image lab v3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) to acquire and analyze imaging data.

    Article Title: LIN28 Is Involved in Glioma Carcinogenesis and Predicts Outcomes of Glioblastoma Multiforme Patients
    Article Snippet: After anti-LIN28 (1∶500) or anti-GAPDH (1∶1000) antibody (Abcom, Cambridge, UK) incubation the membranes were washed in Tris-buffered saline-Tween (TBS-T). .. The membranes were then incubated with a goat anti-mouse horseradish peroxidase-conjugated secondary antibody (1∶20,000, Abcom, Cambridge, UK) for 60 min at room temperature before being washed again in TBS-T. Proteins were visualized with the enhanced chemiluminescence plus (ECL+) system (Amersham) on a FluorChem 8900 imaging system (Alpha Innotech). ..

    Article Title: A single dose of an adenovirus-vectored vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: The plates were then blocked at 37 °C for 1 h with PBS pH 7.4 in 5% skim milk (blocking buffer) and washed with PBST. .. Serial dilutions of sera or trachea-lung washes of mice in dilution buffer were added to the plates and incubated at RT for 1 h. HRP-conjugated goat anti-mouse IgG, IgG1, IgG2a (Abcam, UK, 1:10,000 dilution) or HRP-conjugated goat anti-mouse IgA (Abcam, UK, 1:10,000 dilution) was added to the plates, and the plates were incubated at RT for 1 h and washed with PBST. .. The assay was developed for 10 min at RT with 100 μL of TMB substrate solution (Solarbio, China), stopped by the addition of 50 μL of stop solution (Solarbio, China) and then measured at 450 nm/630 nm (SPECTRA MAX 190, Molecular Device, USA).

    Article Title: CLDN1 Increases Drug Resistance of Non-Small Cell Lung Cancer by Activating Autophagy via Up-Regulation of ULK1 Phosphorylation
    Article Snippet: Afterwards, the samples (5 μl) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 100 V. The resolved proteins were transferred to polyvinylidene difluoride membranes on ice (100 V, 1 h) and blocked with 50 g/L skimmed milk at room temperature for 1 h. Then, the membranes were incubated with rabbit anti-human CLDN1 polyclonal primary antibody (1: 1,000; Abcam, Cambridge, UK) and rabbit anti-human GAPDH primary antibody (1: 5,000; Abcam, Cambridge, UK) at 4°C overnight. .. After extensive washing with phosphate-buffered saline with Tween 20 for 5 times of 5 min each, the membranes were incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (1: 10 000; Abcam, Cambridge, UK) for 1 h at room temperature before washing with phosphate-buffered saline with Tween 20 for 5 times for 5 min each. .. Then, the membrane was developed with enhanced chemiluminescence detection kit (Sigma-Aldrich, St. Louis, MO, USA) for imaging.

    Imaging:

    Article Title: MicroRNA-21 promotes the proliferation, migration and invasion of non-small cell lung cancer A549 cells by regulating autophagy activity via AMPK/ULK1 signaling pathway
    Article Snippet: Then, the proteins were electro-transferred to polyvinylidene difluoride (PVDF) membranes on ice (250 mA, 1 h) before being blocked with 50 g/l skimmed milk at room temperature for 1 h. Afterwards, rabbit anti-human polyclonal ULK1 (1:1,000), LC3B (1:800), AMPKα (1:1,000), p-AMPK (1:800) and p62 (1:800) primary antibodies and mouse anti-human GAPDH (1:400) primary antibody (Abcam, Cambridge, UK) were added onto the membranes before incubation at 4°C overnight. .. Then, the membrane was extensively washed with phosphate-buffered saline with Tween 20 (PBST) for 5 times of 5 min, and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:4,000; Abcam) at room temperature for 1 h. Subsequently, the membrane was washed with PBST for 5 times of 5 min before the membrane was developed with enhanced chemiluminescence detection kit (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for imaging. .. We used Image lab v3.0 software (Bio-Rad Laboratories, Inc., Hercules, CA, USA) to acquire and analyze imaging data.

    Article Title: LIN28 Is Involved in Glioma Carcinogenesis and Predicts Outcomes of Glioblastoma Multiforme Patients
    Article Snippet: After anti-LIN28 (1∶500) or anti-GAPDH (1∶1000) antibody (Abcom, Cambridge, UK) incubation the membranes were washed in Tris-buffered saline-Tween (TBS-T). .. The membranes were then incubated with a goat anti-mouse horseradish peroxidase-conjugated secondary antibody (1∶20,000, Abcom, Cambridge, UK) for 60 min at room temperature before being washed again in TBS-T. Proteins were visualized with the enhanced chemiluminescence plus (ECL+) system (Amersham) on a FluorChem 8900 imaging system (Alpha Innotech). ..

    Mouse Assay:

    Article Title: A single dose of an adenovirus-vectored vaccine provides protection against SARS-CoV-2 challenge
    Article Snippet: The plates were then blocked at 37 °C for 1 h with PBS pH 7.4 in 5% skim milk (blocking buffer) and washed with PBST. .. Serial dilutions of sera or trachea-lung washes of mice in dilution buffer were added to the plates and incubated at RT for 1 h. HRP-conjugated goat anti-mouse IgG, IgG1, IgG2a (Abcam, UK, 1:10,000 dilution) or HRP-conjugated goat anti-mouse IgA (Abcam, UK, 1:10,000 dilution) was added to the plates, and the plates were incubated at RT for 1 h and washed with PBST. .. The assay was developed for 10 min at RT with 100 μL of TMB substrate solution (Solarbio, China), stopped by the addition of 50 μL of stop solution (Solarbio, China) and then measured at 450 nm/630 nm (SPECTRA MAX 190, Molecular Device, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Protection Efficacy of Oral Bait Probiotic Vaccine Constitutively Expressing Tetravalent Toxoids against Clostridium perfringens Exotoxins in Livestock (Rabbits)
    Article Snippet: .. Subsequently, antigen-specific sIgA levels in the intestinal mucus and fecal samples (diluted at 1:16) and antigen-specific IgG levels in serum samples (diluted at 1:32) were detected using ELISA against α, β1, β2, and ε toxoid proteins expressed by E. coli in our laboratory as the coat antigen and using HRP-conjugated goat anti-mouse IgA or IgG (Abcam, Cambridge, MA, USA) as the secondary antibody. .. In addition, IL-2, IL-4, IL-10, IL-12, and IFN-γ cytokine levels in the serum samples obtained from the NZW rabbits in each group were detected using ELISA kits (Biosource International, Waltham, MA, USA).

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  • 95
    Abcam goat anti mouse horseradish peroxidase labeled secondary antibodies
    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using <t>mouse</t> <t>anti-hITF</t> antibodies and <t>goat</t> anti-mouse <t>TRITC-labeled</t> <t>secondary</t> antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.
    Goat Anti Mouse Horseradish Peroxidase Labeled Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse horseradish peroxidase labeled secondary antibodies/product/Abcam
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse horseradish peroxidase labeled secondary antibodies - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    99
    Abcam goat anti mouse hrp secondary antibody
    Conjugation efficiency and biodistribution analysis of CRISPR-modified antibody. ( a ) Coomassie blue stained gel of sortase-mediated conjugation of modified antibody to GGG-fluorophore. ( b ) Fluorescence scan of the antibody-fluorophore, stained with <t>anti-Flag-HRP,</t> showing the antibody conjugated fluorophore (red tab) compared to free fluorophore (black tab). ( c ) HPLC fluorescence trace antibody-fluorophore conjugates at different molar ratios. ( d ) Quantitative analysis of the percent of antibody modification. ( e ) Binding of 125 I-labeled CRISPR-modified antibody to REN-ICAM and REN-WT cells. ( f ) Biodistribution of 125 I-labeled CRISPR-modified anti-ICAM mAb in mice at 30 min. Tissue uptake is indicated as mean ± SEM (n = 3). Biodistribution analysis was performed comparing 111 In-labeled site-specific CRISPR/Cas9- to chemically-modified mAb. ( g ) Biodistribution of 111 In-labeled anti-ICAM mAb modified in mice at 30 min. Tissue uptake is indicated as mean ± SEM. ( h ) Localization ratio of selected organs. Significant differences were determined by t-test with Bonferroni correction to account for multiple comparisons.
    Goat Anti Mouse Hrp Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse hrp secondary antibody/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse hrp secondary antibody - by Bioz Stars, 2021-05
    99/100 stars
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    94
    Abcam horseradish peroxidase hrp labelled goat antimouse igg secondary antibody
    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin <t>IgG</t> and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase <t>(HRP)</t> enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).
    Horseradish Peroxidase Hrp Labelled Goat Antimouse Igg Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp labelled goat antimouse igg secondary antibody/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp labelled goat antimouse igg secondary antibody - by Bioz Stars, 2021-05
    94/100 stars
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    90
    Abcam goat anti mouse horseradish peroxidase hrp light chain secondary antibody
    Detection of gK and UL20 on gradient-purified virions. Western <t>immunoblot</t> analysis of double-gradient-purified virions was performed using anti-V5 (gK) and anti-FLAG (UL20) antibodies. Individual lanes are labeled as follows: C, positive control for GAPDH; W, lysates from purified wild-type virus [HSV-1(F)-YE102]; M, lysates from purified mutant virus YE102-VC1; L, lysates from infected Vero cells; V, samples derived from purified virions. Individual blots were probed with anti-GAPDH, anti-ICP8, anti-gB, anti-gD, anti-gC, anti-FLAG (UL20), or anti-V5 (gK) antibody as indicated. The panel labeled at the top as IP:V5Ab shows immunoblots of immunoprecipitations with anti-V5 (gK) antibody probed with either anti-FLAG (UL20) or anti-V5 (gK) antibodies. In this panel, lanes are immunoprecipitates from mock-infected Vero cells (U), HSV-1(F)-YE102 purified virions (W), or YE102-VC1 purified virions (M). Molecular mass standards are shown with dots on each panel (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa; Precision Plus protein standards; Bio-Rad). <t>HRP-conjugated</t> goat anti-mouse (HRP-GAb; IgG) was used for all data, except data shown on the panel labeled IP:V5Ab, wherein the F(ab) 2 - and Fc-purified portions of the HRP-GAb IgG were used for the α-V5 and α-FLAG panels, respectively.
    Goat Anti Mouse Horseradish Peroxidase Hrp Light Chain Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse horseradish peroxidase hrp light chain secondary antibody/product/Abcam
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse horseradish peroxidase hrp light chain secondary antibody - by Bioz Stars, 2021-05
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    Image Search Results


    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Journal: PLoS ONE

    Article Title: Recombinant Adenovirus-Mediated Intestinal Trefoil Factor Gene Therapy for Burn-Induced Intestinal Mucosal Injury

    doi: 10.1371/journal.pone.0062429

    Figure Lengend Snippet: Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Article Snippet: Mouse anti-hITF antibodies and goat anti-mouse horseradish peroxidase-labeled secondary antibodies were purchased from Abcom (Cambridge, MA, USA).

    Techniques: Infection, Labeling, Staining

    Conjugation efficiency and biodistribution analysis of CRISPR-modified antibody. ( a ) Coomassie blue stained gel of sortase-mediated conjugation of modified antibody to GGG-fluorophore. ( b ) Fluorescence scan of the antibody-fluorophore, stained with anti-Flag-HRP, showing the antibody conjugated fluorophore (red tab) compared to free fluorophore (black tab). ( c ) HPLC fluorescence trace antibody-fluorophore conjugates at different molar ratios. ( d ) Quantitative analysis of the percent of antibody modification. ( e ) Binding of 125 I-labeled CRISPR-modified antibody to REN-ICAM and REN-WT cells. ( f ) Biodistribution of 125 I-labeled CRISPR-modified anti-ICAM mAb in mice at 30 min. Tissue uptake is indicated as mean ± SEM (n = 3). Biodistribution analysis was performed comparing 111 In-labeled site-specific CRISPR/Cas9- to chemically-modified mAb. ( g ) Biodistribution of 111 In-labeled anti-ICAM mAb modified in mice at 30 min. Tissue uptake is indicated as mean ± SEM. ( h ) Localization ratio of selected organs. Significant differences were determined by t-test with Bonferroni correction to account for multiple comparisons.

    Journal: Scientific Reports

    Article Title: Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9

    doi: 10.1038/s41598-018-19784-2

    Figure Lengend Snippet: Conjugation efficiency and biodistribution analysis of CRISPR-modified antibody. ( a ) Coomassie blue stained gel of sortase-mediated conjugation of modified antibody to GGG-fluorophore. ( b ) Fluorescence scan of the antibody-fluorophore, stained with anti-Flag-HRP, showing the antibody conjugated fluorophore (red tab) compared to free fluorophore (black tab). ( c ) HPLC fluorescence trace antibody-fluorophore conjugates at different molar ratios. ( d ) Quantitative analysis of the percent of antibody modification. ( e ) Binding of 125 I-labeled CRISPR-modified antibody to REN-ICAM and REN-WT cells. ( f ) Biodistribution of 125 I-labeled CRISPR-modified anti-ICAM mAb in mice at 30 min. Tissue uptake is indicated as mean ± SEM (n = 3). Biodistribution analysis was performed comparing 111 In-labeled site-specific CRISPR/Cas9- to chemically-modified mAb. ( g ) Biodistribution of 111 In-labeled anti-ICAM mAb modified in mice at 30 min. Tissue uptake is indicated as mean ± SEM. ( h ) Localization ratio of selected organs. Significant differences were determined by t-test with Bonferroni correction to account for multiple comparisons.

    Article Snippet: The membrane was incubated with anti-FLAG M2 antibody (F3165; Sigma-Aldrich, St. Louis, MO), followed by goat anti-mouse HRP secondary antibody (ab6789; Abcam, Cambridge, MA).

    Techniques: Conjugation Assay, CRISPR, Modification, Staining, Fluorescence, High Performance Liquid Chromatography, Binding Assay, Labeling, Mouse Assay

    Schematic diagram and characterization of CRISPR/Cas9 genome editing of hybridoma cells for site-specific modification of antibodies. C-terminal end of Ig gamma-2B gene from ( a ) rat chromosome 13q24, ( b ) Fcgr2b locus, was modified by ( c ) homology directed repair to incorporate sortase and flag tags. sgRNAs with best On-Target/Off-Target scores were selected in the region near the IgG2b stop codon. ( d ) Anti-ICAMIgG2b antibodies were generated incorporating Sortase and FLAG tags at their C-terminal end. Confirmation of insert was performed by ( e ) Agarose gel analysis of PCR fragments from positive and negative clones. ( f ) Verification of integration in positive clones by sanger sequencing. ( g ) Coomassie blue stained gel of three positive clones. ( h ) Western blot of a positive clone under reducing and non-reducing conditions, stained with anti-Flag-HRP.

    Journal: Scientific Reports

    Article Title: Molecular engineering of antibodies for site-specific covalent conjugation using CRISPR/Cas9

    doi: 10.1038/s41598-018-19784-2

    Figure Lengend Snippet: Schematic diagram and characterization of CRISPR/Cas9 genome editing of hybridoma cells for site-specific modification of antibodies. C-terminal end of Ig gamma-2B gene from ( a ) rat chromosome 13q24, ( b ) Fcgr2b locus, was modified by ( c ) homology directed repair to incorporate sortase and flag tags. sgRNAs with best On-Target/Off-Target scores were selected in the region near the IgG2b stop codon. ( d ) Anti-ICAMIgG2b antibodies were generated incorporating Sortase and FLAG tags at their C-terminal end. Confirmation of insert was performed by ( e ) Agarose gel analysis of PCR fragments from positive and negative clones. ( f ) Verification of integration in positive clones by sanger sequencing. ( g ) Coomassie blue stained gel of three positive clones. ( h ) Western blot of a positive clone under reducing and non-reducing conditions, stained with anti-Flag-HRP.

    Article Snippet: The membrane was incubated with anti-FLAG M2 antibody (F3165; Sigma-Aldrich, St. Louis, MO), followed by goat anti-mouse HRP secondary antibody (ab6789; Abcam, Cambridge, MA).

    Techniques: CRISPR, Modification, Generated, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Clone Assay, Sequencing, Staining, Western Blot

    Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin IgG and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase (HRP) enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).

    Journal: Biosensors

    Article Title: Nanoelectrode Arrays Fabricated by Thermal Nanoimprint Lithography for Biosensing Application

    doi: 10.3390/bios10080090

    Figure Lengend Snippet: Schematic representation of the arrays of nanoelectrodes (NEA)-gliadin. The polycarbonate (PC) surface is exploited to immobilize the antigen (Ag) protein, gliadin. The assay is performed by molecular recognition of the target by the primary antibody anti-gliadin IgG and a subsequent secondary antibody anti-IgG labeled with horse radish peroxidase (HRP) enzyme. The electrocatalytic cycle was generated by adding the enzyme substrate H 2 O 2 and the mediator methylene blue (MB).

    Article Snippet: Recombinant Wheat Gliadin protein (19 kDa), mouse monoclonal antigliadin antibody, and horseradish peroxidase (HRP)-labelled goat antimouse IgG secondary antibody were purchased from Abcam® (Cambridge, UK).

    Techniques: Labeling, Generated

    Detection of gK and UL20 on gradient-purified virions. Western immunoblot analysis of double-gradient-purified virions was performed using anti-V5 (gK) and anti-FLAG (UL20) antibodies. Individual lanes are labeled as follows: C, positive control for GAPDH; W, lysates from purified wild-type virus [HSV-1(F)-YE102]; M, lysates from purified mutant virus YE102-VC1; L, lysates from infected Vero cells; V, samples derived from purified virions. Individual blots were probed with anti-GAPDH, anti-ICP8, anti-gB, anti-gD, anti-gC, anti-FLAG (UL20), or anti-V5 (gK) antibody as indicated. The panel labeled at the top as IP:V5Ab shows immunoblots of immunoprecipitations with anti-V5 (gK) antibody probed with either anti-FLAG (UL20) or anti-V5 (gK) antibodies. In this panel, lanes are immunoprecipitates from mock-infected Vero cells (U), HSV-1(F)-YE102 purified virions (W), or YE102-VC1 purified virions (M). Molecular mass standards are shown with dots on each panel (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa; Precision Plus protein standards; Bio-Rad). HRP-conjugated goat anti-mouse (HRP-GAb; IgG) was used for all data, except data shown on the panel labeled IP:V5Ab, wherein the F(ab) 2 - and Fc-purified portions of the HRP-GAb IgG were used for the α-V5 and α-FLAG panels, respectively.

    Journal: Journal of Virology

    Article Title: Site-Specific Proteolytic Cleavage of the Amino Terminus of Herpes Simplex Virus Glycoprotein K on Virion Particles Inhibits Virus Entry ▿

    doi: 10.1128/JVI.06268-11

    Figure Lengend Snippet: Detection of gK and UL20 on gradient-purified virions. Western immunoblot analysis of double-gradient-purified virions was performed using anti-V5 (gK) and anti-FLAG (UL20) antibodies. Individual lanes are labeled as follows: C, positive control for GAPDH; W, lysates from purified wild-type virus [HSV-1(F)-YE102]; M, lysates from purified mutant virus YE102-VC1; L, lysates from infected Vero cells; V, samples derived from purified virions. Individual blots were probed with anti-GAPDH, anti-ICP8, anti-gB, anti-gD, anti-gC, anti-FLAG (UL20), or anti-V5 (gK) antibody as indicated. The panel labeled at the top as IP:V5Ab shows immunoblots of immunoprecipitations with anti-V5 (gK) antibody probed with either anti-FLAG (UL20) or anti-V5 (gK) antibodies. In this panel, lanes are immunoprecipitates from mock-infected Vero cells (U), HSV-1(F)-YE102 purified virions (W), or YE102-VC1 purified virions (M). Molecular mass standards are shown with dots on each panel (250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa; Precision Plus protein standards; Bio-Rad). HRP-conjugated goat anti-mouse (HRP-GAb; IgG) was used for all data, except data shown on the panel labeled IP:V5Ab, wherein the F(ab) 2 - and Fc-purified portions of the HRP-GAb IgG were used for the α-V5 and α-FLAG panels, respectively.

    Article Snippet: Immunoblot assays were carried out using mouse anti-V5 antibody and goat anti-mouse horseradish peroxidase (HRP) light-chain secondary antibody (Abcam, Inc., Cambridge, MA).

    Techniques: Purification, Western Blot, Labeling, Positive Control, Mutagenesis, Infection, Derivative Assay