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Abcam goat anti iga
Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) <t>IgA</t> chimeric antibodies with V1, Nb23 and Nb5, (C) <t>IgY</t> chimeric antibodies with V1, Nb23 and Nb5.
Goat Anti Iga, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter"

Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

Journal: PLoS ONE

doi: 10.1371/journal.pone.0204222

Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.
Figure Legend Snippet: Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.

Techniques Used: Expressing, Positive Control, Molecular Weight

Expression of chimeric antibodies in transgenic A . thaliana seeds. Extracts of T2 seeds were coated and analysed by ELISA. The presence of chimeric antibodies in the extracts was tested, using anti-chicken IgA or IgY conjugated to HRP. As a negative control, extract of wild-type A . thaliana seeds was used. The results of seed extracts from A . thaliana plants transformed with Nb5-IgA, Nb23-IgA, V1-IgA, Nb5-IgY, Nb23-IgY and V1-IgY are shown.
Figure Legend Snippet: Expression of chimeric antibodies in transgenic A . thaliana seeds. Extracts of T2 seeds were coated and analysed by ELISA. The presence of chimeric antibodies in the extracts was tested, using anti-chicken IgA or IgY conjugated to HRP. As a negative control, extract of wild-type A . thaliana seeds was used. The results of seed extracts from A . thaliana plants transformed with Nb5-IgA, Nb23-IgA, V1-IgA, Nb5-IgY, Nb23-IgY and V1-IgY are shown.

Techniques Used: Expressing, Transgenic Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Transformation Assay

Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates. (A, B, C, J, K, L) C . jejuni strain KC40, (D, E, F, M, N, O) C . jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C . coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E . coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.
Figure Legend Snippet: Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates. (A, B, C, J, K, L) C . jejuni strain KC40, (D, E, F, M, N, O) C . jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C . coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E . coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.

Techniques Used: Binding Assay, Negative Control, Microscopy

Binding of chimeric antibodies to C . jejuni KC40 bacteria and purified MOMP. KC40 bacteria and MOMP (1 μ g/ml) were coated in an ELISA plate. Subsequently, the interaction of twofold serial dilutions of the seed extracts was assessed. Therefore, anti-IgA and anti-IgY antibodies, conjugated to HRP, were used for the detection of chimeric antibodies, bound to the bacteria or to the purified native MOMP antigen. As a negative control, the binding of extracts of wild-type A . thaliana seeds with the bacteria and MOMP was measured. (A) Nb5-IgA, (B) Nb23-IgA, (C) Nb5-IgY and (D) Nb23-IgY. The error bars correspond to the standard deviations.
Figure Legend Snippet: Binding of chimeric antibodies to C . jejuni KC40 bacteria and purified MOMP. KC40 bacteria and MOMP (1 μ g/ml) were coated in an ELISA plate. Subsequently, the interaction of twofold serial dilutions of the seed extracts was assessed. Therefore, anti-IgA and anti-IgY antibodies, conjugated to HRP, were used for the detection of chimeric antibodies, bound to the bacteria or to the purified native MOMP antigen. As a negative control, the binding of extracts of wild-type A . thaliana seeds with the bacteria and MOMP was measured. (A) Nb5-IgA, (B) Nb23-IgA, (C) Nb5-IgY and (D) Nb23-IgY. The error bars correspond to the standard deviations.

Techniques Used: Binding Assay, Purification, Enzyme-linked Immunosorbent Assay, Negative Control

Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.
Figure Legend Snippet: Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.

Techniques Used: Expressing, SDS Page, Staining, Western Blot, Transformation Assay, Construct, Plasmid Preparation, Negative Control

Interaction of chimeric antibodies in seed extract from homozygous plants with their antigen. SDS-PAGE and a western blot were performed on purified MOMP under non-denaturing conditions. (A) SDS-PAGE with purified native MOMP stained with Coomassie blue dye. The results of the western blot confirm the interactions of (B) Nb23-IgA14D and (C) Nb23-IgY12C, with MOMP. (D) Wild-type extract was used as a negative control. The western blot was developed with anti-IgA or anti-IgY antibodies conjugated to HRP.
Figure Legend Snippet: Interaction of chimeric antibodies in seed extract from homozygous plants with their antigen. SDS-PAGE and a western blot were performed on purified MOMP under non-denaturing conditions. (A) SDS-PAGE with purified native MOMP stained with Coomassie blue dye. The results of the western blot confirm the interactions of (B) Nb23-IgA14D and (C) Nb23-IgY12C, with MOMP. (D) Wild-type extract was used as a negative control. The western blot was developed with anti-IgA or anti-IgY antibodies conjugated to HRP.

Techniques Used: SDS Page, Western Blot, Purification, Staining, Negative Control

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Incubation:

Article Title: Induction of Salivary Proteins Modifies Measures of Both Orosensory and Postingestive Feedback during Exposure to a Tannic Acid Diet
Article Snippet: .. The membranes were then incubated for 1-h at room temperature with goat anti-IgA antibody (1: 80 000) conjugated with horseradish peroxidase, (Abcam Inc. # ab97185). .. The immunoblotted proteins were detected using Super Signal West Dura Extended Duration Substrate (Thermo Scientific, #34076) and CL-XPosure Film (Pierce Protein Biology Products).

Article Title: Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets
Article Snippet: The endogenous peroxidase activity was neutralized using 3% H2 O2 at 37˚C for 30 min and the sections were rinsed three times with PBS for 15 min. Antigens were unmasked by microwaving (800 W) in citrate buffer (containing 2 mM citric acid and 10 mM trisodium citrate; pH 6.0) for 15 min. .. The sections were then treated with 5% BSA at 37˚C for 1 h to block non-specific binding prior to incubation with rabbit anti-pig CD3 (1:100; cat. no. ab16669; Abcam) or goat anti-pig IgA (1:100; cat. no. ab112639; Abcam) overnight at 4˚C. .. The paraffin sections were rinsed three times with PBS for 15 min and then incubated with biotinylated goat anti-rabbit IgG or rabbit anti-goat IgG at 37˚C for 1 h. The paraffin sections were then rinsed three times with PBS and incubated with HRP labeled SABC at 37˚C.

Article Title: Intrauterine delivery of subunit vaccines induces a systemic and mucosal immune response in rabbits.
Article Snippet: .. Mucosal vaccines have long been sought after to improve protection though the production of both a mucosal and systemic immune response, and are thought to be particularly effective at the site of induction. .. Mucosal vaccines have long been sought after to improve protection though the production of both a mucosal and systemic immune response, and are thought to be particularly effective at the site of induction.

other:

Article Title: Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets
Article Snippet: AntibodiesGoat anti-pig IgA (1:100; cat. no. ab112639) and rabbit anti-pig CD3 (SP7) (1:100; cat. no. ab16669) monoclonal antibodies were purchased from Abcam.

Blocking Assay:

Article Title: Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets
Article Snippet: The endogenous peroxidase activity was neutralized using 3% H2 O2 at 37˚C for 30 min and the sections were rinsed three times with PBS for 15 min. Antigens were unmasked by microwaving (800 W) in citrate buffer (containing 2 mM citric acid and 10 mM trisodium citrate; pH 6.0) for 15 min. .. The sections were then treated with 5% BSA at 37˚C for 1 h to block non-specific binding prior to incubation with rabbit anti-pig CD3 (1:100; cat. no. ab16669; Abcam) or goat anti-pig IgA (1:100; cat. no. ab112639; Abcam) overnight at 4˚C. .. The paraffin sections were rinsed three times with PBS for 15 min and then incubated with biotinylated goat anti-rabbit IgG or rabbit anti-goat IgG at 37˚C for 1 h. The paraffin sections were then rinsed three times with PBS and incubated with HRP labeled SABC at 37˚C.

Binding Assay:

Article Title: Comparison of oral and nasal immunization with inactivated porcine epidemic diarrhea virus on intestinal immunity in piglets
Article Snippet: The endogenous peroxidase activity was neutralized using 3% H2 O2 at 37˚C for 30 min and the sections were rinsed three times with PBS for 15 min. Antigens were unmasked by microwaving (800 W) in citrate buffer (containing 2 mM citric acid and 10 mM trisodium citrate; pH 6.0) for 15 min. .. The sections were then treated with 5% BSA at 37˚C for 1 h to block non-specific binding prior to incubation with rabbit anti-pig CD3 (1:100; cat. no. ab16669; Abcam) or goat anti-pig IgA (1:100; cat. no. ab112639; Abcam) overnight at 4˚C. .. The paraffin sections were rinsed three times with PBS for 15 min and then incubated with biotinylated goat anti-rabbit IgG or rabbit anti-goat IgG at 37˚C for 1 h. The paraffin sections were then rinsed three times with PBS and incubated with HRP labeled SABC at 37˚C.

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    Abcam goat anti human α sma antibody
    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and <t>α-SMA</t> (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.
    Goat Anti Human α Sma Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Abcam goat anti iga
    Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) <t>IgA</t> chimeric antibodies with V1, Nb23 and Nb5, (C) <t>IgY</t> chimeric antibodies with V1, Nb23 and Nb5.
    Goat Anti Iga, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti iga/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    93
    Abcam biotin goat anti mertk
    In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker <t>MerTK</t> in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or <t>HuR</t> siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (
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    95
    Abcam goat anti mouse horseradish peroxidase labeled secondary antibodies
    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using <t>mouse</t> <t>anti-hITF</t> antibodies and <t>goat</t> anti-mouse <t>TRITC-labeled</t> <t>secondary</t> antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.
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    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and α-SMA (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.

    Journal: Molecular Therapy Oncolytics

    Article Title: Inhibition of hypoxia-inducible factor via upregulation of von Hippel-Lindau protein induces “angiogenic switch off” in a hepatoma mouse model

    doi: 10.1038/mto.2015.20

    Figure Lengend Snippet: Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and α-SMA (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.

    Article Snippet: After blocking, sections were incubated with goat anti-human α-SMA antibody (1:100; Abcam) at 4 °C overnight and Alexa fluor 568-labeled anti-goat IgG antibody (1:100; Invitrogen) for 1 hour.

    Techniques: Incubation, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Mouse Assay, Negative Control, Cell Culture, In Vitro, Positive Control, Standard Deviation, Immunofluorescence, Staining

    Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Screening for the expression of chimeric antibodies in A . thaliana ] were used. A His-tagged nanobody was used as a positive control, resulting in a protein band with a molecular weight of approximately 15 kDa. (A) IgA chimeric antibodies with V1, Nb23 and Nb5, (C) IgY chimeric antibodies with V1, Nb23 and Nb5.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Expressing, Positive Control, Molecular Weight

    Expression of chimeric antibodies in transgenic A . thaliana seeds. Extracts of T2 seeds were coated and analysed by ELISA. The presence of chimeric antibodies in the extracts was tested, using anti-chicken IgA or IgY conjugated to HRP. As a negative control, extract of wild-type A . thaliana seeds was used. The results of seed extracts from A . thaliana plants transformed with Nb5-IgA, Nb23-IgA, V1-IgA, Nb5-IgY, Nb23-IgY and V1-IgY are shown.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Expression of chimeric antibodies in transgenic A . thaliana seeds. Extracts of T2 seeds were coated and analysed by ELISA. The presence of chimeric antibodies in the extracts was tested, using anti-chicken IgA or IgY conjugated to HRP. As a negative control, extract of wild-type A . thaliana seeds was used. The results of seed extracts from A . thaliana plants transformed with Nb5-IgA, Nb23-IgA, V1-IgA, Nb5-IgY, Nb23-IgY and V1-IgY are shown.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Expressing, Transgenic Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Transformation Assay

    Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates. (A, B, C, J, K, L) C . jejuni strain KC40, (D, E, F, M, N, O) C . jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C . coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E . coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates. (A, B, C, J, K, L) C . jejuni strain KC40, (D, E, F, M, N, O) C . jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C . coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E . coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Binding Assay, Negative Control, Microscopy

    Binding of chimeric antibodies to C . jejuni KC40 bacteria and purified MOMP. KC40 bacteria and MOMP (1 μ g/ml) were coated in an ELISA plate. Subsequently, the interaction of twofold serial dilutions of the seed extracts was assessed. Therefore, anti-IgA and anti-IgY antibodies, conjugated to HRP, were used for the detection of chimeric antibodies, bound to the bacteria or to the purified native MOMP antigen. As a negative control, the binding of extracts of wild-type A . thaliana seeds with the bacteria and MOMP was measured. (A) Nb5-IgA, (B) Nb23-IgA, (C) Nb5-IgY and (D) Nb23-IgY. The error bars correspond to the standard deviations.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Binding of chimeric antibodies to C . jejuni KC40 bacteria and purified MOMP. KC40 bacteria and MOMP (1 μ g/ml) were coated in an ELISA plate. Subsequently, the interaction of twofold serial dilutions of the seed extracts was assessed. Therefore, anti-IgA and anti-IgY antibodies, conjugated to HRP, were used for the detection of chimeric antibodies, bound to the bacteria or to the purified native MOMP antigen. As a negative control, the binding of extracts of wild-type A . thaliana seeds with the bacteria and MOMP was measured. (A) Nb5-IgA, (B) Nb23-IgA, (C) Nb5-IgY and (D) Nb23-IgY. The error bars correspond to the standard deviations.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Binding Assay, Purification, Enzyme-linked Immunosorbent Assay, Negative Control

    Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Transient expression of chimeric antibodies carrying Nb5 in leaves of N . benthamiana . Protein extracts were analysed using (A) SDS-PAGE stained with Coomassie blue dye and (B) western blot developed with mouse anti-histidine tag monoclonal antibody. Extracts of N . benthamiana leaves transformed with Nb5-IgA and Nb5-IgY fusion constructs were tested. Leaves infiltrated with A . tumefaciens harbouring the vector pEAQ -HT were used as negative control.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: Expressing, SDS Page, Staining, Western Blot, Transformation Assay, Construct, Plasmid Preparation, Negative Control

    Interaction of chimeric antibodies in seed extract from homozygous plants with their antigen. SDS-PAGE and a western blot were performed on purified MOMP under non-denaturing conditions. (A) SDS-PAGE with purified native MOMP stained with Coomassie blue dye. The results of the western blot confirm the interactions of (B) Nb23-IgA14D and (C) Nb23-IgY12C, with MOMP. (D) Wild-type extract was used as a negative control. The western blot was developed with anti-IgA or anti-IgY antibodies conjugated to HRP.

    Journal: PLoS ONE

    Article Title: In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

    doi: 10.1371/journal.pone.0204222

    Figure Lengend Snippet: Interaction of chimeric antibodies in seed extract from homozygous plants with their antigen. SDS-PAGE and a western blot were performed on purified MOMP under non-denaturing conditions. (A) SDS-PAGE with purified native MOMP stained with Coomassie blue dye. The results of the western blot confirm the interactions of (B) Nb23-IgA14D and (C) Nb23-IgY12C, with MOMP. (D) Wild-type extract was used as a negative control. The western blot was developed with anti-IgA or anti-IgY antibodies conjugated to HRP.

    Article Snippet: Subsequently, the western blot was developed using goat anti-IgA (1/5000) or goat anti-IgY (1/1250) conjugated to HRP (Abcam).

    Techniques: SDS Page, Western Blot, Purification, Staining, Negative Control

    In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker MerTK in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or HuR siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (

    Journal: Nature Communications

    Article Title: A macrophage-specific lncRNA regulates apoptosis and atherosclerosis by tethering HuR in the nucleus

    doi: 10.1038/s41467-020-19664-2

    Figure Lengend Snippet: In vivo knockdown of MAARS inhibits plaque necrosis and increases efferocytosis. a Quantification of plaque necrosis in the lesions of the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (knockdown (KD), n = 13). b Flow cytometry staining with the efferocytosis marker MerTK in BMDMs treated with or without 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells for 1 h at a BMDM:AC ratio of 1:10. c High resolution confocal images and quantification of MerTK staining in BMDMs treated with 20 µM CPT for 2 h and incubated with Jurkat apoptotic cells (AC) for 1 h at a BMDM:AC ratio of 1:1. d Evaluation of macrophage efferocytosis in vivo by co-staining with MerTK and Mac-3, and quantification of the ratio between double-positive MerTK + /Mac3 + and Mac3 staining alone in the aortic sinus of LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmers (KD, n = 13). e Quantification of the ratio between free TUNEL staining (arrow heads) vs. Mac3-positive TUNEL staining (arrows), reflecting Mac3-positive efferocytotic macrophages, in the aortic sinus lesions LDLR −/− mice fed HCD treated with control ( n = 15) or MAARS gapmeRs (KD, n = 13). f In vitro efferocytosis assay: BMDMs were transfected with control or MAARS gapmeRs in the presence or absence of the indicated control or HuR siRNAs and subsequently incubated with calcein AM-labeled apoptotic Jurkat cells for 1 h. Efferocytosis was quantified as the ratio of calcein-positive (

    Article Snippet: Sections were stained with rat anti-Mac2, (Cedarlane, CL8942AP, 1:100), anti-Mac3 (BD Pharmingen, 553322, 1:900), rabbit anti-cleaved caspase-3 (Cell Signaling, 9664, 1:400) rabbit anti-HuR/ELAVL1 (12582S Cell Signaling and AB242410, Abcam), biotin goat anti-MerTK (BAF591, R & D Technologies) in PBS at 4 °C overnight.

    Techniques: In Vivo, Mouse Assay, Flow Cytometry, Staining, Marker, Incubation, TUNEL Assay, In Vitro, Transfection, Labeling

    Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Journal: PLoS ONE

    Article Title: Recombinant Adenovirus-Mediated Intestinal Trefoil Factor Gene Therapy for Burn-Induced Intestinal Mucosal Injury

    doi: 10.1371/journal.pone.0062429

    Figure Lengend Snippet: Fluorescent images of HT-29 cells infected with Ad-hITF. The hITF was detected using mouse anti-hITF antibodies and goat anti-mouse TRITC-labeled secondary antibodies. Nuclei were stained with DAPI. A, GFP; B, hITF; C, cell nuclei; D, merged images.

    Article Snippet: Mouse anti-hITF antibodies and goat anti-mouse horseradish peroxidase-labeled secondary antibodies were purchased from Abcom (Cambridge, MA, USA).

    Techniques: Infection, Labeling, Staining