goat anti human alpha smooth muscle actin α sma polyclonal antibody  (Abcam)

 
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    Goat polyclonal Anti Human IgM SureLight Allophycocyanin
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    Catalog Number:
    AB99899
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    Structured Review

    Abcam goat anti human alpha smooth muscle actin α sma polyclonal antibody

    https://www.bioz.com/result/goat anti human alpha smooth muscle actin α sma polyclonal antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human alpha smooth muscle actin α sma polyclonal antibody - by Bioz Stars, 2021-07
    93/100 stars

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    Immunoprecipitation:

    Article Title: Antagonism of the complement component C4 by flavivirus nonstructural protein NS1
    Article Snippet: Western blot was performed using anti-DENV NS1 mAb (1F11) hybridoma supernatant and HRP-conjugated anti–mouse IgG. .. Anti–human C4 Ab-coated beads were prepared by adding 5 µg of goat anti–human C4 polyclonal Ab (Abcam) to 50 µl of TrueBlot anti–goat Ig immunoprecipitation beads (eBioscience). .. Erythrocyte hemolysis assay The assay was performed by a stepwise buildup of complement intermediates on EA according to previous work ( ).

    Article Title: Interaction of Complement Factor H and Fibulin3 in Age-Related Macular Degeneration
    Article Snippet: For immunoprecipitation, CFH (Complement Technology, Inc., Tyler, TX) at 0, 2 or 5 µg/ml was added on day 5 of serum deprivation. .. Immunoprecipitation Antibodies used were goat polyclonal anti-human CFH, horse radish peroxidase (HRP) conjugated polyclonal donkey anti-goat IgG (Abcam Inc., Cambridge, MA), goat anti-rabbit IgG-HRP (Bio-Rad Laboratories, Hercules, CA). .. A new anti Fib3 antibody was raised in rabbits immunized against peptide NNEQPQQETQPAEGTSG (21st Century Biochemicals, Inc., Marlboro, MA).

    Incubation:

    Article Title: Effects of Payena dasyphylla (Miq.) on hyaluronidase enzyme activity and metalloproteinases protein expressions in interleukin-1? stimulated human chondrocytes cells
    Article Snippet: .. Next, the membrane was incubated with a blocking solution PBST containing 5% serum for 1 hour at 37°C, and then probed with goat anti-human polyclonal antibodies against MMP-3 (1:1000) (Abcam, USA) and MMP-13 (1:1000) (Santa Cruz, USA) separately overnight. ..

    Article Title: Activated Platelets Interfere with Recruitment of Mesenchymal Stem Cells to Apoptotic Cardiac Cells via High Mobility Group Box 1/Toll-like Receptor 4-mediated Down-regulation of Hepatocyte Growth Factor Receptor MET *
    Article Snippet: MSC were grown for 24 h on coverslips in 24-well plates in culture medium and subsequently incubated for a further 24 h with supernatant of ADP- or CRP-activated platelets supplemented with 10% FCS (CM) or in PBS supplemented with 10% FCS. .. Cells were fixed with 2% paraformaldehyde, blocked with 1% BSA-PBS for 1 h, incubated overnight at 4 °C with anti-human MET polyclonal antibody (1:500 dilution; rabbit IgG; Abcam), washed with PBS + 0.1% Tween 20, and incubated with Alexa Fluor 488-tagged goat anti-rabbit IgG (1:100) for 2 h at room temperature. .. Following another washing step, nuclei were stained by incubation with TO-PRO-3 iodide, coverslips were mounted with antifade fluorescence mounting medium, and confocal microscopic analysis was performed as described above.

    Blocking Assay:

    Article Title: Effects of Payena dasyphylla (Miq.) on hyaluronidase enzyme activity and metalloproteinases protein expressions in interleukin-1? stimulated human chondrocytes cells
    Article Snippet: .. Next, the membrane was incubated with a blocking solution PBST containing 5% serum for 1 hour at 37°C, and then probed with goat anti-human polyclonal antibodies against MMP-3 (1:1000) (Abcam, USA) and MMP-13 (1:1000) (Santa Cruz, USA) separately overnight. ..

    Expressing:

    Article Title: ≤ Cyclin D1 protein affecting global women’s health by regulating HPV mediated adenocarcinoma of the uterine cervix
    Article Snippet: .. Polyclonal anti-human antibodies of Cyclin D1, β-actin (rabbit monoclonal β-actin antibody (1:2000, Abcam) and diluted secondary antibody HRP-conjugated rabbit anti-IgG (Abcam,) were used to determine the expression levels of Cyclin D1 in tumor tissues. .. Expression levels were further quantitated and compared in normal tissues and were evaluated by densitometry using Alpha Digidoc version 4.1.0 (Alpha Innotech Corporation, IL) as described in our previous reports , .

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  • 93
    Abcam goat anti human alpha smooth muscle actin α sma polyclonal antibody
    Goat Anti Human Alpha Smooth Muscle Actin α Sma Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human alpha smooth muscle actin α sma polyclonal antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human alpha smooth muscle actin α sma polyclonal antibody - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

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