goat anti human α sma antibody  (Abcam)

 
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    Name:
    Goat Anti Human IgA alpha chain Glucose Oxidase
    Description:

    Catalog Number:
    AB136785
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    Structured Review

    Abcam goat anti human α sma antibody
    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and <t>α-SMA</t> (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.

    https://www.bioz.com/result/goat anti human α sma antibody/product/Abcam
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human α sma antibody - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Inhibition of hypoxia-inducible factor via upregulation of von Hippel-Lindau protein induces “angiogenic switch off” in a hepatoma mouse model"

    Article Title: Inhibition of hypoxia-inducible factor via upregulation of von Hippel-Lindau protein induces “angiogenic switch off” in a hepatoma mouse model

    Journal: Molecular Therapy Oncolytics

    doi: 10.1038/mto.2015.20

    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and α-SMA (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.
    Figure Legend Snippet: Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and α-SMA (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.

    Techniques Used: Incubation, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Mouse Assay, Negative Control, Cell Culture, In Vitro, Positive Control, Standard Deviation, Immunofluorescence, Staining

    Related Articles

    Blocking Assay:

    Article Title: Inhibition of hypoxia-inducible factor via upregulation of von Hippel-Lindau protein induces “angiogenic switch off” in a hepatoma mouse model
    Article Snippet: Immunohistochemistry Immunohistochemical staining of CD31 and TdT-mediated dUTP nick end labeling staining was performed as previously described., For double immunofluorescence examination of CD31 and α-smooth muscle actin (α-SMA), sections were incubated at 4 °C overnight with rabbit anti-human CD31 antibody (Abcam) and fluorescein isothiocyanate–conjugated anti-rabbit IgG antibody (1:100; Invitrogen, Carlsbad, CA) for 1 hour. .. After blocking, sections were incubated with goat anti-human α-SMA antibody (1:100; Abcam) at 4 °C overnight and Alexa fluor 568-labeled anti-goat IgG antibody (1:100; Invitrogen) for 1 hour. .. Nuclei were counterstained with TO-PRO-3 iodide (1:1,000, Invitrogen).

    Incubation:

    Article Title: Inhibition of hypoxia-inducible factor via upregulation of von Hippel-Lindau protein induces “angiogenic switch off” in a hepatoma mouse model
    Article Snippet: Immunohistochemistry Immunohistochemical staining of CD31 and TdT-mediated dUTP nick end labeling staining was performed as previously described., For double immunofluorescence examination of CD31 and α-smooth muscle actin (α-SMA), sections were incubated at 4 °C overnight with rabbit anti-human CD31 antibody (Abcam) and fluorescein isothiocyanate–conjugated anti-rabbit IgG antibody (1:100; Invitrogen, Carlsbad, CA) for 1 hour. .. After blocking, sections were incubated with goat anti-human α-SMA antibody (1:100; Abcam) at 4 °C overnight and Alexa fluor 568-labeled anti-goat IgG antibody (1:100; Invitrogen) for 1 hour. .. Nuclei were counterstained with TO-PRO-3 iodide (1:1,000, Invitrogen).

    Article Title: Giardia intestinalis and Fructose Malabsorption: A Frequent Association
    Article Snippet: Samples diluted 1:2 in PBS-BSA were dispensed in duplicate (100 µL/well) and incubated for 90 min at 37 °C. .. After three washes with PBST, an incubation with the secondary antibody, peroxidase-conjugated anti-human IgA (goat polyclonal anti-human IgA alpha chain, Abcam, Cambridge, MA, USA), in a dilution of 1:10,000 in PBS-BSA was made for 1 h at 37 °C. .. Wells were washed three times with PBST and substrate solution (0.04% of ortho-phenylenediamine (OPD) and 0.001% of hydrogen peroxide in 0.05 phosphate (0.2 M)‒citrate (0.1 M) buffer, pH 5.0 (Sigma Aldrich)) was added (100 µL/well) for 10 min in the darkness and the reaction stopped with HCl (3N) (100 µL/well).

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  • 92
    Abcam goat anti human α sma antibody
    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and <t>α-SMA</t> (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.
    Goat Anti Human α Sma Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human α sma antibody/product/Abcam
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human α sma antibody - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    93
    Abcam anti human sma igg
    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and <t>α-SMA</t> (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.
    Anti Human Sma Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human sma igg/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti human sma igg - by Bioz Stars, 2021-06
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    93
    Abcam goat anti human alpha smooth muscle actin α sma polyclonal antibody
    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and <t>α-SMA</t> (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.
    Goat Anti Human Alpha Smooth Muscle Actin α Sma Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human alpha smooth muscle actin α sma polyclonal antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti human alpha smooth muscle actin α sma polyclonal antibody - by Bioz Stars, 2021-06
    93/100 stars
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    Image Search Results


    Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and α-SMA (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.

    Journal: Molecular Therapy Oncolytics

    Article Title: Inhibition of hypoxia-inducible factor via upregulation of von Hippel-Lindau protein induces “angiogenic switch off” in a hepatoma mouse model

    doi: 10.1038/mto.2015.20

    Figure Lengend Snippet: Multiple mechanisms decreasing HIFα in sulfoquinovosyl-acylpropanediol (SQAP). ( a ) The presence of the proteasome inhibitor MG-132 decreased the effect of SQAP on HIFα protein levels. HAK1-B cells incubated under either normoxia or 3% O 2 hypoxia were treated with 10 μmo/l SQAP for 24 hours and further incubated for 4 hours in the presence of 10 μmo/l MG-132. ( b ) HIF1α synthesis at the transcriptional level decreased upon SQAP addition in a dose-dependent manner. HAK-1B cells were incubated with medium containing different dose of SQAP (1 and 10 μmo/l). Cellular RNA was then extracted and analyzed for HIF1α mRNA expression by quantitative real-time polymerase chain reaction, with GAPDH as a control. ( c ) SQAP decreases NFκB expression in HAK1-B and Huh-7. Western blots for both cell lines exposed to SQAP (1 and 10 μmo/l) under hypoxic conditions are shown. Cells were incubated with SQAP-containing medium for 24 hours, after which the cells were moved to 3% O 2 hypoxic conditions for 24 hours and then lysed with radioimmunoprecipitation buffer. ( d ) Representative immunohistochemistry images for pimonidazole in HAK1-B tumor treated with SQAP. Scale bar = 200 μm. ( e ) Western blot analysis of pimonidazole adducts in HAK1-B tumors treated with SQAP. Whole tumors were collected from tumor bearing mice ( n = 4 per group). Negative control: HAK1-B cells cultured under normoxia in vitro . Positive control: HAK1-B cell cultured under 3% O 2 hypoxia in vitro . Pimonidazole adducts are shown in the indicated multiple bands. The band densities for each protein were measured and calibrated by β-actin. Average bands densities are represented as mean ± standard deviation (SD). ( f ) Representative double immunofluorescence images using both CD31 (green) and α-SMA (red) in HAK1-B tumors treated with SQAP. Nuclei were stained with DAPI. Scale bar = 50 μm. Relative number of tumor vessels, α-SMA-positive cells, and tumor vessels covered with α-SMA positive cells in HAK1-B tumors treated with SQAP are shown. The number of tumor vessels and the ratio of pericyte covered vessels are represented as mean ± SD. DAPI, 4’,6-diamidino-2-phenylindole, dihydrochloride.

    Article Snippet: After blocking, sections were incubated with goat anti-human α-SMA antibody (1:100; Abcam) at 4 °C overnight and Alexa fluor 568-labeled anti-goat IgG antibody (1:100; Invitrogen) for 1 hour.

    Techniques: Incubation, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunohistochemistry, Mouse Assay, Negative Control, Cell Culture, In Vitro, Positive Control, Standard Deviation, Immunofluorescence, Staining