goat anti β galactosidase  (Millipore)


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    Structured Review

    Millipore goat anti β galactosidase
    Subchronic T 3 treatment enhances the number of <t>β-galactosidase</t> immunopositive cells within the neocortex of Shh +/LacZ mice. Shown are representative images of β-galactosidase expressing cells in the cortex after short-duration T 3 treatment over 2 d in Shh +/LacZ mice. T 3 treatment significantly increased the number of cells that were strongly immunopositive for β-galactosidase in layer V of cortex (A). Results are expressed as a percentage of vehicle-treated control and are the mean ± sem (n = 5/group). *, P
    Goat Anti β Galactosidase, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti β galactosidase/product/Millipore
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti β galactosidase - by Bioz Stars, 2020-08
    89/100 stars

    Images

    1) Product Images from "Thyroid Hormone Regulates the Expression of the Sonic Hedgehog Signaling Pathway in the Embryonic and Adult Mammalian Brain"

    Article Title: Thyroid Hormone Regulates the Expression of the Sonic Hedgehog Signaling Pathway in the Embryonic and Adult Mammalian Brain

    Journal: Endocrinology

    doi: 10.1210/en.2010-1396

    Subchronic T 3 treatment enhances the number of β-galactosidase immunopositive cells within the neocortex of Shh +/LacZ mice. Shown are representative images of β-galactosidase expressing cells in the cortex after short-duration T 3 treatment over 2 d in Shh +/LacZ mice. T 3 treatment significantly increased the number of cells that were strongly immunopositive for β-galactosidase in layer V of cortex (A). Results are expressed as a percentage of vehicle-treated control and are the mean ± sem (n = 5/group). *, P
    Figure Legend Snippet: Subchronic T 3 treatment enhances the number of β-galactosidase immunopositive cells within the neocortex of Shh +/LacZ mice. Shown are representative images of β-galactosidase expressing cells in the cortex after short-duration T 3 treatment over 2 d in Shh +/LacZ mice. T 3 treatment significantly increased the number of cells that were strongly immunopositive for β-galactosidase in layer V of cortex (A). Results are expressed as a percentage of vehicle-treated control and are the mean ± sem (n = 5/group). *, P

    Techniques Used: Mouse Assay, Expressing

    Related Articles

    IA:

    Article Title: Thyroid Hormone Regulates the Expression of the Sonic Hedgehog Signaling Pathway in the Embryonic and Adult Mammalian Brain
    Article Snippet: .. In brief, sections were incubated with primary antibody cocktails of goat anti-β-galactosidase with mouse anti-NeuN (1:500; Millipore Corp., Bedford, MA) or mouse anti-RIP (1:10; Developmental Studies Hybridoma Bank, Iowa City, IA) along with rabbit anti-NG2 (1:250; Millipore Corp.) or rabbit anti-GFAP (1:250; Millipore Corp.). .. After incubation with primary antibody for 3 d at 4 C, sections were washed and incubated with a cocktail of secondary antibodies: Alexa Fluor 488-conjugated antigoat (1:250); rhodamine-conjugated antimouse IgG (1:500; Millipore Corp.), and Cy-5-conjugated antirabbit IgG (1:500; Millipore Corp.) for 2 h. Sections were mounted onto slides with Vectashield (Vector Laboratories, Burlingame, CA) and analyzed using a Zeiss Axioplan2 confocal laser scanning microscope (Zeiss, Oberkochen, Germany).

    Incubation:

    Article Title: Thyroid Hormone Regulates the Expression of the Sonic Hedgehog Signaling Pathway in the Embryonic and Adult Mammalian Brain
    Article Snippet: .. In brief, sections were incubated with primary antibody cocktails of goat anti-β-galactosidase with mouse anti-NeuN (1:500; Millipore Corp., Bedford, MA) or mouse anti-RIP (1:10; Developmental Studies Hybridoma Bank, Iowa City, IA) along with rabbit anti-NG2 (1:250; Millipore Corp.) or rabbit anti-GFAP (1:250; Millipore Corp.). .. After incubation with primary antibody for 3 d at 4 C, sections were washed and incubated with a cocktail of secondary antibodies: Alexa Fluor 488-conjugated antigoat (1:250); rhodamine-conjugated antimouse IgG (1:500; Millipore Corp.), and Cy-5-conjugated antirabbit IgG (1:500; Millipore Corp.) for 2 h. Sections were mounted onto slides with Vectashield (Vector Laboratories, Burlingame, CA) and analyzed using a Zeiss Axioplan2 confocal laser scanning microscope (Zeiss, Oberkochen, Germany).

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  • 89
    Millipore goat anti β galactosidase
    Subchronic T 3 treatment enhances the number of <t>β-galactosidase</t> immunopositive cells within the neocortex of Shh +/LacZ mice. Shown are representative images of β-galactosidase expressing cells in the cortex after short-duration T 3 treatment over 2 d in Shh +/LacZ mice. T 3 treatment significantly increased the number of cells that were strongly immunopositive for β-galactosidase in layer V of cortex (A). Results are expressed as a percentage of vehicle-treated control and are the mean ± sem (n = 5/group). *, P
    Goat Anti β Galactosidase, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti β galactosidase/product/Millipore
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti β galactosidase - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    85
    Millipore goat anti mouse immunoglobulin β galactosidase conjugate
    Representative morphophogies of MAb A20 and MAb 76.3 (inset) AAV-2 pseudo-plaques. HeLa cells were infected with AAV-2/Ad-2 stock. Following fixing, sites of AAV-2 infection were revealed through addition of one of the two AAV-specific MAbs used as a primary antibody, then degradation of X-gal by <t>β-galactosidase</t> linked to a secondary antibody. The A20-based assay, specific for intact capsids, was performed 24 h post-infection, whereas MAb 76.3 detection, that is specific for replication protein, was performed after 48 h to allow larger plaque development. Smaller “satellite” foci often surround primary plaques, likely result from some diffusion of progeny virus away from the initial site of infection, are not counted as distinct plaques in quantitative assays.
    Goat Anti Mouse Immunoglobulin β Galactosidase Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse immunoglobulin β galactosidase conjugate/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse immunoglobulin β galactosidase conjugate - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Subchronic T 3 treatment enhances the number of β-galactosidase immunopositive cells within the neocortex of Shh +/LacZ mice. Shown are representative images of β-galactosidase expressing cells in the cortex after short-duration T 3 treatment over 2 d in Shh +/LacZ mice. T 3 treatment significantly increased the number of cells that were strongly immunopositive for β-galactosidase in layer V of cortex (A). Results are expressed as a percentage of vehicle-treated control and are the mean ± sem (n = 5/group). *, P

    Journal: Endocrinology

    Article Title: Thyroid Hormone Regulates the Expression of the Sonic Hedgehog Signaling Pathway in the Embryonic and Adult Mammalian Brain

    doi: 10.1210/en.2010-1396

    Figure Lengend Snippet: Subchronic T 3 treatment enhances the number of β-galactosidase immunopositive cells within the neocortex of Shh +/LacZ mice. Shown are representative images of β-galactosidase expressing cells in the cortex after short-duration T 3 treatment over 2 d in Shh +/LacZ mice. T 3 treatment significantly increased the number of cells that were strongly immunopositive for β-galactosidase in layer V of cortex (A). Results are expressed as a percentage of vehicle-treated control and are the mean ± sem (n = 5/group). *, P

    Article Snippet: In brief, sections were incubated with primary antibody cocktails of goat anti-β-galactosidase with mouse anti-NeuN (1:500; Millipore Corp., Bedford, MA) or mouse anti-RIP (1:10; Developmental Studies Hybridoma Bank, Iowa City, IA) along with rabbit anti-NG2 (1:250; Millipore Corp.) or rabbit anti-GFAP (1:250; Millipore Corp.).

    Techniques: Mouse Assay, Expressing

    Representative morphophogies of MAb A20 and MAb 76.3 (inset) AAV-2 pseudo-plaques. HeLa cells were infected with AAV-2/Ad-2 stock. Following fixing, sites of AAV-2 infection were revealed through addition of one of the two AAV-specific MAbs used as a primary antibody, then degradation of X-gal by β-galactosidase linked to a secondary antibody. The A20-based assay, specific for intact capsids, was performed 24 h post-infection, whereas MAb 76.3 detection, that is specific for replication protein, was performed after 48 h to allow larger plaque development. Smaller “satellite” foci often surround primary plaques, likely result from some diffusion of progeny virus away from the initial site of infection, are not counted as distinct plaques in quantitative assays.

    Journal: Journal of virological methods

    Article Title: A pseudo-Plaque method for Infectious Particle Assay and Clonal Isolation of Adeno-associated Virus

    doi: 10.1016/j.jviromet.2010.08.004

    Figure Lengend Snippet: Representative morphophogies of MAb A20 and MAb 76.3 (inset) AAV-2 pseudo-plaques. HeLa cells were infected with AAV-2/Ad-2 stock. Following fixing, sites of AAV-2 infection were revealed through addition of one of the two AAV-specific MAbs used as a primary antibody, then degradation of X-gal by β-galactosidase linked to a secondary antibody. The A20-based assay, specific for intact capsids, was performed 24 h post-infection, whereas MAb 76.3 detection, that is specific for replication protein, was performed after 48 h to allow larger plaque development. Smaller “satellite” foci often surround primary plaques, likely result from some diffusion of progeny virus away from the initial site of infection, are not counted as distinct plaques in quantitative assays.

    Article Snippet: Following 3 TBS-T washes of 5 minutes each, the secondary antibody was added (goat-anti mouse immunoglobulin / β-galactosidase conjugate, Calbiochem, Inc.) at 1:100 dilution in TBS and incubated for 1 hour.

    Techniques: Infection, Diffusion-based Assay