gnl3 expression plasmids  (Sino Biological)

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: Effects of GNL3 knockdown and overexpression on the levels of the EMT-related markers, β-catenin and GNL3 in vitro . (A and B) In HCT-116 cells, GNL3 knockdown (GNL3 shRNA) increased E-cadherin levels and reduced N-cadherin, vimentin, nuclear β-catenin (β-catenin-N) and GNL3 levels compared with controls (Con). (A and C) In HT-29 cells, GNL3 overexpression (GNL3) increased the N-cadherin, vimentin, β-catenin-N and GNL3 levels and reduced the E-cadherin levels compared with the negative controls (NC). **P

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Over Expression, In Vitro, shRNA

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: Treatment with suitable concentrations of LiCl activates the Wnt/β-catenin signaling pathway, and LiCl partially reverses the GNL3 knockdown-induced inhibition of the EMT and Wnt/β-catenin signaling pathway in HCT-116 cells. (A and B) Both 20 and 40 mM LiCl were appropriate concentrations to activate the Wnt/β-catenin signaling pathway. (C and D) GNL3 knockdown (GNL3 shRNA) increased E-cadherin expression and decreased N-cadherin, vimentin, nuclear β-catenin (β-catenin-N) and GNL3 expression compared with the controls (Con). LiCl increased N-cadherin, vimentin and β-catenin-N expression and reduced E-cadherin expression compared with the GNL3 shRNA group. *P

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Inhibition, shRNA, Expressing

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: Immunofluorescence staining for β-catenin. The β-catenin proteins labeled in red and the nuclei were stained with DAPI and are labeled in blue. GNL3 knockdown (GNL3 shRNA) in HCT-116 cells reduced the nuclear β-catenin levels compared with the controls (Con). However, GNL3 overexpression (GNL3) in HT-29 cells increased the nuclear β-catenin levels compared with the negative controls (NC). Original magnification, ×400.

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Immunofluorescence, Staining, Labeling, shRNA, Over Expression

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: Effects of GNL3 knockdown and overexpression on cell invasion and migration in vitro . (A) Following GNL3 knockdown (GNL3 shRNA), the invasive capacity of HCT-116 cells was decreased compared with those of control cells (Con). Following GNL3 overexpression (GNL3), the invasive capacity of HT-29 cells was increased compared with negative control cells (NC). (B) GNL3 knockdown inhibited the migration of HCT-116 cells, and GNL3 overexpression promoted the migration of HT-29 cells. *P

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Over Expression, Migration, In Vitro, shRNA, Negative Control

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: GNL3 expression and its clinical significance in colon cancer. (A) Representative images of immunohistochemical staining for GNL3 in normal, tumor-adjacent tissues and colon cancer tissues. Original magnification, ×200. (B) GNL3 levels in normal, tumor-adjacent tissues (N1-4) and colon cancer tissues (T1-4) were detected by western blotting. According to the quantitative analysis, GNL3 was expressed at significantly higher levels in colon cancer tissues than in normal, tumor-adjacent tissues. (C and D) Overall 5-year and disease-free survival curves for GNL3-negative and GNL3-positive patients with colon cancer according to the Kaplan-Meier analysis. The overall and disease-free survival rates of the GNL3-positive groups were markedly reduced compared with the GNL3-negative groups. **P

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: GNL3 expression was assessed in different colon cancer cell lines using western blotting. (A) GNL3 levels in five colon cancer cell lines: HCT-116, HT-29, SW620, Caco-2 and LoVo cells. (B) GNL3 expression was significantly reduced in GNL3 knockdown cells (GNL3 shRNA) compared with that in the control cells (Con) and was substantially increased in GNL3-overexpressing cells (GNL3) compared with that in the negative control cells (NC). **P

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Expressing, Western Blot, shRNA, Negative Control

Journal: Oncology Reports

Article Title: Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway

doi: 10.3892/or.2017.5923

Figure Lengend Snippet: Effects of GNL3 knockdown and overexpression on cell proliferation and colony formation in vitro and tumor growth in nude mice in vivo . (A) In HCT-116 cells, the proliferation rates of the GNL3 knockdown group (GNL3 shRNA) were lower than the control group (Con). (B) In HT-29 cells, the proliferation rates of the GNL3-overexpressing group (GNL3) were much higher than in the negative control group (NC). (C) GNL3 knockdown inhibited the formation of HCT-116 cell colonies, and GNL3 overexpression promoted the formation of HT-29 cell colonies. (D and E) Representative xenograft tumors are shown and the tumor volume was measured weekly. *P

Article Snippet: GNL3 knockdown plasmids were purchased from Sigma-Aldrich (St. Louis, MO, USA; TRCN0000293740) and GNL3 expression plasmids were purchased from Sino Biological Inc. (Beijing, China; HG12415-UT).

Techniques: Over Expression, In Vitro, Mouse Assay, In Vivo, shRNA, Negative Control