glyceraldehyde 3 phosphate dehydrogenase gapdh  (Proteintech)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Proteintech glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; <t>GAPDH=glyceraldehyde-3-phosphate</t> dehydrogenase
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-02
    94/100 stars

    Images

    1) Product Images from "DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43"

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43

    Journal: Brazilian Journal of Cardiovascular Surgery

    doi: 10.21470/1678-9741-2019-0033

    Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; GAPDH=glyceraldehyde-3-phosphate dehydrogenase
    Figure Legend Snippet: Effects of DACT1 on β-catenin in myocardial cells. Effect of DACT1 overexpression on β-catenin concentration A) and distribution B) in H9C2 and HL-1 cells. C) Correlation between DACT1 and β-catenin expression in the myocardial tissues of patients with valvular heart disease. D) Colocation of DACT1 and β-catenin in the myocardial cells. H9C2 and HL-1 cells were overexpressed with the two isoforms of the DACT1 coding sequence, and then immunofluorescence was used to detect the DACT1 (DACT1-V1 or DACT1-V2) (OriGene) and β-catenin (CST) location. The secondary antibody Alexa Fluor 647 was used for DACT1 detection and Alexa Fluor 568 for β-catenin. The ZEN (Zeiss) software was used for further analysis. Alexa Fluor 647 was represented by the green color and Alexa Fluor 568 by the red color, while channel 488 was closed to avoid interference. DACT1=dishevelled binding antagonist of beta catenin 1; DAPI=2-(4-amidinophenyl)-6-indolecarbamidine dihydrochloride; GAPDH=glyceraldehyde-3-phosphate dehydrogenase

    Techniques Used: Over Expression, Concentration Assay, Expressing, Sequencing, Immunofluorescence, Software, Binding Assay

    The effect of DACT1 on Cx43 in myocardial cells. The effect of DACT1 overexpression on Cx43 concentration A) and distribution B) in H9C2 and HL-1 cells. C) Cx43 expression in the myocardial tissues of patients with valvular heart disease. DACT1=dishevelled binding antagonist of beta catenin 1; GAPDH=glyceraldehyde-3-phosphate dehydrogenase
    Figure Legend Snippet: The effect of DACT1 on Cx43 in myocardial cells. The effect of DACT1 overexpression on Cx43 concentration A) and distribution B) in H9C2 and HL-1 cells. C) Cx43 expression in the myocardial tissues of patients with valvular heart disease. DACT1=dishevelled binding antagonist of beta catenin 1; GAPDH=glyceraldehyde-3-phosphate dehydrogenase

    Techniques Used: Over Expression, Concentration Assay, Expressing, Binding Assay

    2) Product Images from "MicroRNA-29a Suppresses CD36 to Ameliorate High Fat Diet-Induced Steatohepatitis and Liver Fibrosis in Mice"

    Article Title: MicroRNA-29a Suppresses CD36 to Ameliorate High Fat Diet-Induced Steatohepatitis and Liver Fibrosis in Mice

    Journal: Cells

    doi: 10.3390/cells8101298

    Overexpression of miR-29a reduces liver fibrosis in the context of chronic HFD. Paraformaldehyde-fixed paraffin-embedded liver tissue was used to determine collagen fiber accumulation using Mason’s trichrome stain. Liver tissue stored at −80 ℃ was used for RNA and protein extraction for subsequent qPCR and Western blot experiments, respectively. ( A ) Representative Masson’s trichrome stain image of each group. Blue color indicates positive signal of collagen fiber. ( B ) Positive signal percentage quantified using ImageJ. ( C ) mRNA expression level of col1 α1 , with β-actin level as normalization control. ( D ) Representative immunoblotting bands of COL1α1 protein abundance and densitometric results, with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the loading control. For the imaging study, data were collected from five fields of view of each specimen and five to eight specimens for each group. For qPCR and Western blot, five to seven specimens were used for each group. Data are expressed as mean ± SE. * p
    Figure Legend Snippet: Overexpression of miR-29a reduces liver fibrosis in the context of chronic HFD. Paraformaldehyde-fixed paraffin-embedded liver tissue was used to determine collagen fiber accumulation using Mason’s trichrome stain. Liver tissue stored at −80 ℃ was used for RNA and protein extraction for subsequent qPCR and Western blot experiments, respectively. ( A ) Representative Masson’s trichrome stain image of each group. Blue color indicates positive signal of collagen fiber. ( B ) Positive signal percentage quantified using ImageJ. ( C ) mRNA expression level of col1 α1 , with β-actin level as normalization control. ( D ) Representative immunoblotting bands of COL1α1 protein abundance and densitometric results, with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the loading control. For the imaging study, data were collected from five fields of view of each specimen and five to eight specimens for each group. For qPCR and Western blot, five to seven specimens were used for each group. Data are expressed as mean ± SE. * p

    Techniques Used: Over Expression, Staining, Protein Extraction, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Imaging

    3) Product Images from "Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1"

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1

    Journal: Journal of Virology

    doi: 10.1128/JVI.01896-17

    CoVs nsp1 inhibit host protein synthesis. (A) PK-15 cells were cotransfected with pRL-TK carrying the Rluc reporter gene downstream of the TK promoter and one of the plasmids pcaggs, pcaggs-PEDV-nsp1-HA (PEDV nsp1), pcaggs-TGEV-nsp1-HA (TGEV nsp1), or pcaggs-SARS-CoV-nsp1-HA (SARS nsp1), which express PEDV nsp1, TGEV nsp1 and SARS-CoV nsp1, respectively. All the expressed proteins had N-terminal HA tags. At 24 h posttransfection, cell lysates were prepared and subjected to Rluc assays. Error bars show the standard deviations (SDs) of the results from three independent experiments. Cell extracts were also subjected to Western blot analysis using an anti-HA antibody (top) or an anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (bottom). *, P
    Figure Legend Snippet: CoVs nsp1 inhibit host protein synthesis. (A) PK-15 cells were cotransfected with pRL-TK carrying the Rluc reporter gene downstream of the TK promoter and one of the plasmids pcaggs, pcaggs-PEDV-nsp1-HA (PEDV nsp1), pcaggs-TGEV-nsp1-HA (TGEV nsp1), or pcaggs-SARS-CoV-nsp1-HA (SARS nsp1), which express PEDV nsp1, TGEV nsp1 and SARS-CoV nsp1, respectively. All the expressed proteins had N-terminal HA tags. At 24 h posttransfection, cell lysates were prepared and subjected to Rluc assays. Error bars show the standard deviations (SDs) of the results from three independent experiments. Cell extracts were also subjected to Western blot analysis using an anti-HA antibody (top) or an anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (bottom). *, P

    Techniques Used: Western Blot

    4) Product Images from "Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice"

    Article Title: Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-15222-x

    Energy metabolism is perturbed in the BCO2 KO liver. ( A ) Expression of proteins associated with energy metabolism, particularly lipid metabolism by immunoblotting. ( B ) Quantifications of listed proteins. ( C ) Metabolites associated with lipid metabolism. ( D ) Heat map of cofactors and vitamins associated with energy metabolism. Heat map only presents the significantly changes metabolites. Values are means ± SD. Data was analyzed by one-way ANOVA. n = 6. Different letters indicate significant difference between groups. P-value ≤ 0.05 is considered as significant. AMPKα, AMP activated protein kinase α; PPARα, peroxisome proliferator activated receptor α; FASN, fatty acid sunthase; HADH, mitochondrial hydroxyacyl-coenzyme A dehydrogenase; ACADL, long-chain specific acyl-CoA dehydrogenase; CPT1A, carnitine palmitoyltransferase 1α; CACT1, carnitine/acylcarnitine translocase 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ATP5A1, ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit 1; W and WT, wild type; H and Het, BCO2 heterozygous mice; K and KO, BCO2 knockout mice.
    Figure Legend Snippet: Energy metabolism is perturbed in the BCO2 KO liver. ( A ) Expression of proteins associated with energy metabolism, particularly lipid metabolism by immunoblotting. ( B ) Quantifications of listed proteins. ( C ) Metabolites associated with lipid metabolism. ( D ) Heat map of cofactors and vitamins associated with energy metabolism. Heat map only presents the significantly changes metabolites. Values are means ± SD. Data was analyzed by one-way ANOVA. n = 6. Different letters indicate significant difference between groups. P-value ≤ 0.05 is considered as significant. AMPKα, AMP activated protein kinase α; PPARα, peroxisome proliferator activated receptor α; FASN, fatty acid sunthase; HADH, mitochondrial hydroxyacyl-coenzyme A dehydrogenase; ACADL, long-chain specific acyl-CoA dehydrogenase; CPT1A, carnitine palmitoyltransferase 1α; CACT1, carnitine/acylcarnitine translocase 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ATP5A1, ATP synthase, H + transporting, mitochondrial F1 complex, alpha subunit 1; W and WT, wild type; H and Het, BCO2 heterozygous mice; K and KO, BCO2 knockout mice.

    Techniques Used: Expressing, Mouse Assay, Knock-Out

    Cholesterol and bile acid metabolism is perturbed in KO liver. ( A ), plasma cholesterol level is low in KO mice. ( B ), expression of HMGCR is decreased in KO liver. ( C ), significantly changes metabolites associated with sterol and bile acid metabolism. Heat map only presents the significantly changes metabolites. Values are means ± SD. Data was analyzed by one-way ANOVA. n = 6. Different letters indicate significant difference between groups. P-value ≤ 0.05 is considered as significant. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; W and WT, wild type; H and Het, BCO2 heterozygous mice; K and KO, BCO2 knockout mice.
    Figure Legend Snippet: Cholesterol and bile acid metabolism is perturbed in KO liver. ( A ), plasma cholesterol level is low in KO mice. ( B ), expression of HMGCR is decreased in KO liver. ( C ), significantly changes metabolites associated with sterol and bile acid metabolism. Heat map only presents the significantly changes metabolites. Values are means ± SD. Data was analyzed by one-way ANOVA. n = 6. Different letters indicate significant difference between groups. P-value ≤ 0.05 is considered as significant. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; W and WT, wild type; H and Het, BCO2 heterozygous mice; K and KO, BCO2 knockout mice.

    Techniques Used: Mouse Assay, Expressing, Knock-Out

    Related Articles

    Centrifugation:

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: The cell pellet was collected after centrifugation and washed. .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: Total protein isolation and Western Blot The cells were washed 3 times with ice-cold PBS and lysed with RIPA buffer (solarbio) containing phosphatase inhibitor on ice for 30 min. After ultrasonic lysis and centrifugation at 12 000 rpm at 4 ℃ for 15 min, the supernatant lysate with proteins was collected. .. The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech).

    Reporter Assay:

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: Paragraph title: Reporter assay and Western blot analysis. ... The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Blocking Assay:

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Next, 10% non‐fat dry milk was used to block nonspecific binding. .. The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Article Title: Non-coding RNA NEAT1/miR-214-3p contribute to doxorubicin resistance of urothelial bladder cancer preliminary through the Wnt/β-catenin pathway
    Article Snippet: .. Then after blocking with 5% non-fat milk, the membranes were hybridized with specific antibodies against P-glycoprotein (P-gp) from Santa Cruz Biotechnology Inc., Dallas, TX, USA; Axin2, glycogen synthase kinase 3 beta (GSK-3β), β-catenin and phospho-β-catenin (Ser675) (p-β-catenin) from Cell Signaling Technology, Danvers, MA, USA; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from ProteinTech, USA (Thermo Fisher Scientific). ..

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: Five percent skim milk in Tris Buffered Saline Tween-20 (TBST) was used to block the membranes for 1 h at room temperature. .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    Electrophoresis:

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: SDS-PAGE and blotting were performed using Bio-Rad electrophoresis and blotting systems (Bio-Rad, Wuhan, China). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Article Title: MicroRNA-29a Suppresses CD36 to Ameliorate High Fat Diet-Induced Steatohepatitis and Liver Fibrosis in Mice
    Article Snippet: Western Blotting We mixed 30-µg protein extracts with a sample buffer, boiled them for 10 min, and performed electrophoresis using 8–15% sodium dodecyl sulfate-polyacrylamide gels. .. We transferred the proteins in the gels to a polyvinylidene difluoride membrane and incubated the blots with primary antibodies against COL1α1 (sc-8784, Santa Cruz, CA, USA), PPARγ (16643-1-AP, PROTEINTECH, IL), TFAM (sc-166965, Santa Cruz, CA, USA), CD36/SR-B3 (NB400-144, Novus Biologicals, CO, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1-lg, PROTEINTECH, IL, USA) for protein control.

    Incubation:

    Article Title: Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix, et al. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
    Article Snippet: Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (1:5000; Proteintech) was used as an internal control. .. Following primary antibody incubation, membranes were washed three times in 0.1% TBST and incubated in goat anti‐rabbit IgG (H & L)‐horseradish peroxidase conjugate (1:2000; Proteintech) 2 hours at room temperature.

    Article Title: CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib
    Article Snippet: .. The membranes were incubated overnight at 4 °C using the following antibodies: CCBE1 (Sigma Life Science, USA, HPA041374, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL). .. After incubating with the IRDye 680 anti-mouse (LI-COR, Lincoln, NE) and IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) secondary antibodies for 1 hour at room temperature, the bands were detected by an Odyssey infrared imaging system (LI-COR, Lincoln, NE).

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Following washing, the membranes were incubated with secondary antibody conjugated with horseradish peroxidase anti‐mouse/rabbit immunoglobulin G (Boster Biological Technology Co. Ltd., Pleasanton, CA, USA). .. The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: The membrane was blocked with 5% nonfat-dried milk solution at room temperature for 1 h and incubated with primary antibodies overnight at 4 ℃. .. The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech).

    Article Title: MicroRNA-29a Suppresses CD36 to Ameliorate High Fat Diet-Induced Steatohepatitis and Liver Fibrosis in Mice
    Article Snippet: .. We transferred the proteins in the gels to a polyvinylidene difluoride membrane and incubated the blots with primary antibodies against COL1α1 (sc-8784, Santa Cruz, CA, USA), PPARγ (16643-1-AP, PROTEINTECH, IL), TFAM (sc-166965, Santa Cruz, CA, USA), CD36/SR-B3 (NB400-144, Novus Biologicals, CO, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1-lg, PROTEINTECH, IL, USA) for protein control. .. After washing the blots with tris-buffered saline and incubating them with horseradish peroxidase-coupled anti-rabbit immunoglobulin-G antibodies (dilution, 1:5000, NEF812001EA, PerkinElmer, MA, USA), HRP anti-mouse immunoglobulin-G antibodies (dilution, 1:10,000, NEF822001, PerkinElmer, MA, USA),), and HRP anti-goat immunoglobulin-G antibodies (dilution, 1:10,000, sc-2354, Santa Cruz, CA, USA) at room temperature for 1 h, we developed them with enhanced chemiluminescence detection (GE Healthcare Biosciences AB, Uppsala, Sweden), exposed them to film, and quantified the signals by using densitometry.

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: .. After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature. .. Bands were detected by an Odyssey infrared imaging system (LI-COR, Lincoln, NE) and quantification was analyzed using Image J software.

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA). .. After washing with TBST, the membranes were incubated with appropriate horse radish peroxidase (HRP)-conjugated secondary antibodies for 1 h at room temperature.

    Activity Assay:

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: At 24 h posttransfection, cell lysates were prepared and subjected to Rluc reporter activity assays (Promega). .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Expressing:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech. .. The expression of target genes was normalized to that of GAPDH or β-actin.

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading. .. In brief, 100 ng of RNA was transferred to separate tubes for amplifying the target Rluc mRNA and the endogenous control (18S rRNA) to use with a TaqMan one-step real-time PCR master mix reagent kit.

    BIA-KA:

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Protease inhibitors, phenylmethanesulfonyl fluoride, the BCA protein assay kit, SDS-polyacrylamide gel electrophoresis and Red Blood Cell Lysis buffer were purchased from Beyotime (Shanghai, China). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: Western Blotting Hypothalamic tissues and cell samples were dissociated in 200 μL of lysis buffer consisting of 1% phenylmethanesulfonyl fluoride (PMSF) (KGP610, KeyGEN, Nanjing, China), and the protein concentration was quantified by a BCA assay kit (Beyotime Biotech Inc., Shanghai, China). .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    Modification:

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Dulbecco’s modified Eagle’s medium, fetal bovine serum (FBS), Roswell Park Memorial Institute 1640 medium (RPMI 1640) and Hank’s balanced salt solution were obtained from Wisent (Montreal, QC, Canada). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    Western Blot:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Paragraph title: Western Blotting ... Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: Paragraph title: Reporter assay and Western blot analysis. ... The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: Paragraph title: 4.5. Western Blot Analysis ... Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Article Title: Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix, et al. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
    Article Snippet: Paragraph title: Protein extraction and Western blotting ... Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (1:5000; Proteintech) was used as an internal control.

    Article Title: CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib
    Article Snippet: Paragraph title: Western blotting ... The membranes were incubated overnight at 4 °C using the following antibodies: CCBE1 (Sigma Life Science, USA, HPA041374, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL).

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: Protein extraction and Western blot ... The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Article Title: A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study
    Article Snippet: Paragraph title: Membrane fraction isolation and Western blot ... The following antibodies were used: CaV 1.2α1C (Alomone Labs, Jerusalem, Israel), ATP1A2 (Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech).

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA). .. Antibodies against α-SMA and TGF-β1 were obtained from Abcam (Cambridge, MA, USA).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: Paragraph title: Total protein isolation and Western Blot ... The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech).

    Article Title: MicroRNA-29a Suppresses CD36 to Ameliorate High Fat Diet-Induced Steatohepatitis and Liver Fibrosis in Mice
    Article Snippet: Paragraph title: 2.7. Western Blotting ... We transferred the proteins in the gels to a polyvinylidene difluoride membrane and incubated the blots with primary antibodies against COL1α1 (sc-8784, Santa Cruz, CA, USA), PPARγ (16643-1-AP, PROTEINTECH, IL), TFAM (sc-166965, Santa Cruz, CA, USA), CD36/SR-B3 (NB400-144, Novus Biologicals, CO, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1-lg, PROTEINTECH, IL, USA) for protein control.

    Article Title: Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice
    Article Snippet: To maximize the utilization of the immunoblotting membranes, the blots at least were cut into two parts according to the molecular weights of the target proteins and reused for a couples of times after being stripped with Thermo Scientific Restore PLUS Western Blot Stripping Buffer (Thermo Scientific Pierce, Rockford, IL, USA). .. Antibodies against mammalian/mechanistic target of rapamycin complex 1 (mTORC1), citrate synthase (CS), carnitine/acylcarnitine translocase 1 (CACT1), ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1(ATP5A1); NADH:ubiquinone oxidoreductase subunit A9 (NDUFA9); iron-sulfur cluster assembly enzyme (ISCU), nicotinamide nucleotide transhydrogenase (NNT), 60 kDa heat shock protein (HSP60), hydroxyacyl-coenzyme A dehydrogenase (HADH), long-chain specific acyl-CoA dehydrogenase (ACADL), carnitine palmitoyltransferase I (CPT1A), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin were purchased from ProteinTech Group (Rosemont, IL, USA).

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: Paragraph title: Western blot ... After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature.

    Article Title: Non-coding RNA NEAT1/miR-214-3p contribute to doxorubicin resistance of urothelial bladder cancer preliminary through the Wnt/β-catenin pathway
    Article Snippet: Paragraph title: Western blot ... Then after blocking with 5% non-fat milk, the membranes were hybridized with specific antibodies against P-glycoprotein (P-gp) from Santa Cruz Biotechnology Inc., Dallas, TX, USA; Axin2, glycogen synthase kinase 3 beta (GSK-3β), β-catenin and phospho-β-catenin (Ser675) (p-β-catenin) from Cell Signaling Technology, Danvers, MA, USA; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from ProteinTech, USA (Thermo Fisher Scientific).

    Article Title: DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling
    Article Snippet: Paragraph title: Western blotting ... The primary antibodies used included the following: DPT (HuaAn Biotechnology, Hangzhou, China), focal adhesion kinase (FAK) (Abcam, Cambridge, UK), p-FAK Tyr397 (Abcam, Cambridge, UK), Src (Cell Signaling Technology, Boston, MA), p-Src Tyr527 (Cell Signaling Technology, Boston, MA), ITGA3 (Abcam, Cambridge, UK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL).

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: Paragraph title: 2.9. Western Blotting ... Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    Transfection:

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: In brief, to analyze the expression levels of nsp1 proteins, PK-15 cells were transfected with various plasmids using the same method. .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Article Title: A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study
    Article Snippet: Briefly, 24 hours after transfection, the protein samples (separate membrane and cytosolic samples) were extracted from cells using the temperature-induced phase separation method. .. The following antibodies were used: CaV 1.2α1C (Alomone Labs, Jerusalem, Israel), ATP1A2 (Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech).

    Protease Inhibitor:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Proteins were isolated from cells with lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China) that included a protease inhibitor cocktail (Millipore, Billerica, Massachusetts, USA). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Cell Culture:

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: The cells were cultured in TSB medium at 37 °C to early stationary phase. .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Imaging:

    Article Title: CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib
    Article Snippet: The membranes were incubated overnight at 4 °C using the following antibodies: CCBE1 (Sigma Life Science, USA, HPA041374, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL). .. After incubating with the IRDye 680 anti-mouse (LI-COR, Lincoln, NE) and IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) secondary antibodies for 1 hour at room temperature, the bands were detected by an Odyssey infrared imaging system (LI-COR, Lincoln, NE).

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature. .. Bands were detected by an Odyssey infrared imaging system (LI-COR, Lincoln, NE) and quantification was analyzed using Image J software.

    Article Title: DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling
    Article Snippet: The primary antibodies used included the following: DPT (HuaAn Biotechnology, Hangzhou, China), focal adhesion kinase (FAK) (Abcam, Cambridge, UK), p-FAK Tyr397 (Abcam, Cambridge, UK), Src (Cell Signaling Technology, Boston, MA), p-Src Tyr527 (Cell Signaling Technology, Boston, MA), ITGA3 (Abcam, Cambridge, UK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL). .. After incubating with the IRDye 680 anti-mouse (LI-COR, Lincoln, NE) and IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) secondary antibodies for 1 hour at room temperature, the bands were detected by an Odyssey infrared imaging system (LI-COR, Lincoln, NE).

    Protein Concentration:

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Protein extraction and Western blot Proteins were extracted from the tissue samples and cell lines, and the total protein concentration was determined using a Pierce bicinchoninic acid Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: Western Blotting Hypothalamic tissues and cell samples were dissociated in 200 μL of lysis buffer consisting of 1% phenylmethanesulfonyl fluoride (PMSF) (KGP610, KeyGEN, Nanjing, China), and the protein concentration was quantified by a BCA assay kit (Beyotime Biotech Inc., Shanghai, China). .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    Binding Assay:

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Next, 10% non‐fat dry milk was used to block nonspecific binding. .. The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Equal amounts of protein (30 μg) were fractionated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and were transferred onto polyvinylidene difluoride membrane filters (EMD Millipore, Billerica, MA, USA). .. The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Article Title: A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study
    Article Snippet: A total of 100 μg lysates or subcellular fractions were separated using 6 to 10% sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE), and then transferred to polyvinylidene fluoride (PVDF) membranes and probed with specific antibodies. .. The following antibodies were used: CaV 1.2α1C (Alomone Labs, Jerusalem, Israel), ATP1A2 (Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech).

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Protease inhibitors, phenylmethanesulfonyl fluoride, the BCA protein assay kit, SDS-polyacrylamide gel electrophoresis and Red Blood Cell Lysis buffer were purchased from Beyotime (Shanghai, China). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    Article Title: Non-coding RNA NEAT1/miR-214-3p contribute to doxorubicin resistance of urothelial bladder cancer preliminary through the Wnt/β-catenin pathway
    Article Snippet: Cells were lysed and protein concentrations were determined as previously described., In total, 30 µg protein were processed including protein separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred into a polyvinylidene fluoride membrane. .. Then after blocking with 5% non-fat milk, the membranes were hybridized with specific antibodies against P-glycoprotein (P-gp) from Santa Cruz Biotechnology Inc., Dallas, TX, USA; Axin2, glycogen synthase kinase 3 beta (GSK-3β), β-catenin and phospho-β-catenin (Ser675) (p-β-catenin) from Cell Signaling Technology, Danvers, MA, USA; glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from ProteinTech, USA (Thermo Fisher Scientific).

    Isolation:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Proteins were isolated from cells with lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China) that included a protease inhibitor cocktail (Millipore, Billerica, Massachusetts, USA). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Article Title: A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study
    Article Snippet: Paragraph title: Membrane fraction isolation and Western blot ... The following antibodies were used: CaV 1.2α1C (Alomone Labs, Jerusalem, Israel), ATP1A2 (Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: Paragraph title: Total protein isolation and Western Blot ... The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech).

    Bicinchoninic Acid Protein Assay:

    Article Title: Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix, et al. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
    Article Snippet: Total protein concentrations were measured using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific Inc), and equal amounts of proteins samples (30 µg/lane) were resolved by SDS‐PAGE and then transferred onto polyvinylidene difluoride membranes, and membranes blocked by incubating with 5% non‐fat milk containing 0.1% tris Buffered saline Tween (TBST) for 2 hours at room temperature. .. Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (1:5000; Proteintech) was used as an internal control.

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Protein extraction and Western blot Proteins were extracted from the tissue samples and cell lines, and the total protein concentration was determined using a Pierce bicinchoninic acid Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). .. The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Immunohistochemistry:

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA). .. Col I and MMP13 were manufactured by Bioworlde (Louis Park, BD, USA) for immunohistochemistry (IHC) analysis.

    Protein Extraction:

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: After one freeze-thaw cycle, total protein was extracted using the Bacterial Protein Extraction Kit from Jiangsu KeyGEN BioTECH, Nanjing, China. .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Article Title: Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix, et al. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
    Article Snippet: Paragraph title: Protein extraction and Western blotting ... Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (1:5000; Proteintech) was used as an internal control.

    Article Title: CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib
    Article Snippet: Western blotting Fresh GIST tissues were lysed in tissue protein extraction reagent (Invitrogen). .. The membranes were incubated overnight at 4 °C using the following antibodies: CCBE1 (Sigma Life Science, USA, HPA041374, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL).

    Article Title: Blocking FGFR4 exerts distinct anti‐tumorigenic effects in esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: Protein extraction and Western blot ... The antibodies used in this study were as follows: FGFR4 (1:1000; Santa Cruz Biotechnology); N‐cadherin, Vimentin, Snail, ERK, p‐ERK, p‐FGFR4, and Bcl‐2 (1:1000; Abcam, Cambridge, MA, USA); E‐cadherin (1:1000; Affbiotech, Cincinnati, OH, USA); Claudin‐1, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH), and Bax (1:1000; Proteintech, Chicago, IL, USA); β‐actin (1:1000; Boster Biological Technology Co. Ltd.); and AKT and p‐AKT (1:1000; Omimabs, Alhambra, CA, USA).

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: The total proteins of HCC tissue samples were extracted using T-Per tissue protein extraction reagent (Thermo Scientific) according to its protocol and total proteins of HCC cells were obtained by IP lysis buffer (Beyotime). .. After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature.

    Stripping:

    Article Title: Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice
    Article Snippet: To maximize the utilization of the immunoblotting membranes, the blots at least were cut into two parts according to the molecular weights of the target proteins and reused for a couples of times after being stripped with Thermo Scientific Restore PLUS Western Blot Stripping Buffer (Thermo Scientific Pierce, Rockford, IL, USA). .. Antibodies against mammalian/mechanistic target of rapamycin complex 1 (mTORC1), citrate synthase (CS), carnitine/acylcarnitine translocase 1 (CACT1), ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1(ATP5A1); NADH:ubiquinone oxidoreductase subunit A9 (NDUFA9); iron-sulfur cluster assembly enzyme (ISCU), nicotinamide nucleotide transhydrogenase (NNT), 60 kDa heat shock protein (HSP60), hydroxyacyl-coenzyme A dehydrogenase (HADH), long-chain specific acyl-CoA dehydrogenase (ACADL), carnitine palmitoyltransferase I (CPT1A), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin were purchased from ProteinTech Group (Rosemont, IL, USA).

    Polyacrylamide Gel Electrophoresis:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Proteins were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, Massachusetts, USA). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Roche, Mannheim, Germany). .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    Lysis:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Proteins were isolated from cells with lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China) that included a protease inhibitor cocktail (Millipore, Billerica, Massachusetts, USA). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: The cells were harvested by adding radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime), and the extracts were prepared in SDS-PAGE sample buffer. .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Article Title: Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix, et al. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
    Article Snippet: 2.6 Protein extraction and Western blotting The cells were washed twice with PBS and lysed in lysis buffer (50 mmol/L Tris‐HCl, pH 8.0, 1 mmol/L EDTA, 1% Triton X‐100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate [SDS], 150 mmol/L NaCl) containing a mixture of proteinase inhibitors (Thermo Fisher Scientific Inc, Rockford, IL). .. Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (1:5000; Proteintech) was used as an internal control.

    Article Title: CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib
    Article Snippet: Primary GIST cells were lysed in Western and IP lysis buffer (P0013, Beyotime, Jiangsu, China) supplemented with 1 mM PMSF (Adamas beta, Shanghai, China). .. The membranes were incubated overnight at 4 °C using the following antibodies: CCBE1 (Sigma Life Science, USA, HPA041374, 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL).

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Radio-immunoprecipitation assay lysis buffer and nitrocellulose membranes were obtained from Millipore (Billerica, MA, USA). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: Total protein isolation and Western Blot The cells were washed 3 times with ice-cold PBS and lysed with RIPA buffer (solarbio) containing phosphatase inhibitor on ice for 30 min. After ultrasonic lysis and centrifugation at 12 000 rpm at 4 ℃ for 15 min, the supernatant lysate with proteins was collected. .. The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech).

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: The total proteins of HCC tissue samples were extracted using T-Per tissue protein extraction reagent (Thermo Scientific) according to its protocol and total proteins of HCC cells were obtained by IP lysis buffer (Beyotime). .. After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature.

    Article Title: DNA methylation-mediated silencing of matricellular protein dermatopontin promotes hepatocellular carcinoma metastasis by α3β1 integrin-Rho GTPase signaling
    Article Snippet: Western blotting Whole cell lysates were prepared by lysis buffer (50mM Tris-HCl, 150mM NaCl, 1% Triton-X 100, 1mM MgCl2 , MnCl2 and CaCl2 , 1mM PMSF and 10mM sodium fluoride) [ ]. .. The primary antibodies used included the following: DPT (HuaAn Biotechnology, Hangzhou, China), focal adhesion kinase (FAK) (Abcam, Cambridge, UK), p-FAK Tyr397 (Abcam, Cambridge, UK), Src (Cell Signaling Technology, Boston, MA), p-Src Tyr527 (Cell Signaling Technology, Boston, MA), ITGA3 (Abcam, Cambridge, UK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL).

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: Western Blotting Hypothalamic tissues and cell samples were dissociated in 200 μL of lysis buffer consisting of 1% phenylmethanesulfonyl fluoride (PMSF) (KGP610, KeyGEN, Nanjing, China), and the protein concentration was quantified by a BCA assay kit (Beyotime Biotech Inc., Shanghai, China). .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    SDS Page:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Proteins were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, Massachusetts, USA). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech.

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: The cells were harvested by adding radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime), and the extracts were prepared in SDS-PAGE sample buffer. .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: SDS-PAGE and blotting were performed using Bio-Rad electrophoresis and blotting systems (Bio-Rad, Wuhan, China). .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Article Title: Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix, et al. Exosomes from tendon stem cells promote injury tendon healing through balancing synthesis and degradation of the tendon extracellular matrix
    Article Snippet: Total protein concentrations were measured using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific Inc), and equal amounts of proteins samples (30 µg/lane) were resolved by SDS‐PAGE and then transferred onto polyvinylidene difluoride membranes, and membranes blocked by incubating with 5% non‐fat milk containing 0.1% tris Buffered saline Tween (TBST) for 2 hours at room temperature. .. Glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) (1:5000; Proteintech) was used as an internal control.

    Article Title: A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study
    Article Snippet: A total of 100 μg lysates or subcellular fractions were separated using 6 to 10% sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE), and then transferred to polyvinylidene fluoride (PVDF) membranes and probed with specific antibodies. .. The following antibodies were used: CaV 1.2α1C (Alomone Labs, Jerusalem, Israel), ATP1A2 (Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: Then proteins were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes. .. The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech).

    Article Title: Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice
    Article Snippet: Equal amounts of liver protein samples were separated by sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE) and then followed by immunoblotting as described previously , . .. Antibodies against mammalian/mechanistic target of rapamycin complex 1 (mTORC1), citrate synthase (CS), carnitine/acylcarnitine translocase 1 (CACT1), ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1(ATP5A1); NADH:ubiquinone oxidoreductase subunit A9 (NDUFA9); iron-sulfur cluster assembly enzyme (ISCU), nicotinamide nucleotide transhydrogenase (NNT), 60 kDa heat shock protein (HSP60), hydroxyacyl-coenzyme A dehydrogenase (HADH), long-chain specific acyl-CoA dehydrogenase (ACADL), carnitine palmitoyltransferase I (CPT1A), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin were purchased from ProteinTech Group (Rosemont, IL, USA).

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: For Western blot analysis, protein lysates were subjected to SDS-PAGE and transferred to nitrocellulose membrane (Millipore). .. After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature.

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Roche, Mannheim, Germany). .. Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA).

    Software:

    Article Title: DACT1 Involvement in the Cytoskeletal Arrangement of Cardiomyocytes in Atrial Fibrillation by Regulating Cx43
    Article Snippet: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech) or β-actin (Wuhan GoodBio Tech. .. Image J software was used to analyze the mean light density of each band.

    Article Title: A mutation in the CACNA1C gene leads to early repolarization syndrome with incomplete penetrance: A Chinese family study
    Article Snippet: The following antibodies were used: CaV 1.2α1C (Alomone Labs, Jerusalem, Israel), ATP1A2 (Proteintech) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech). .. Signals of blots were collected through a Bio-RAD ChemiDox XRS detection system and analyzed by Quantity One analysis software (Bio-Rad, Hercules, CA, USA).

    Article Title: The Crosstalk between HDPSCs and HUCMSCs on Proliferation and Osteogenic Genes Expression in Coculture System
    Article Snippet: The following primary antibodies were used: CDK6 (1:1000, CST), CYCLIN A (1:1000, CST), Akt (pan) (1:1000, CST), phospho-Akt (Thr308) (p-Akt) (1:1000 CST), mTOR (1:1000, CST), phospho-mTOR (p-mTOR) (1:1000, CST), COL I (1:1000, abcam), Runx2 (1:1000, CST), OPN (1:1000, Santa), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000, Proteintech). .. Finally, the protein bands were detected by enhanced chemiluminescence (Millipore) and band intensities were analyzed by Image J software.

    Article Title: Cytohesin-3 is upregulated in hepatocellular carcinoma and contributes to tumor growth and vascular invasion
    Article Snippet: After blocked with 5% no-fat milk (BD), the membrane was incubated with primary antibody for cytohesin-3 (Abcam, Cambridge, UK) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Proteintech Group, Chicago IL) at 4°C overnight and followed by incubation of secondary antibodies IRDye 800 anti-rabbit (LI-COR, Lincoln, NE) or IRDye 680 anti-mouse (LI-COR, Lincoln, NE) for 1 hour at room temperature. .. Bands were detected by an Odyssey infrared imaging system (LI-COR, Lincoln, NE) and quantification was analyzed using Image J software.

    Article Title: MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model
    Article Snippet: Following this, the membranes were incubated with the following primary antibodies at 4 °C overnight: PSD95 (1:1000, abcam, Cat# ab12093, Cambridge, UK), Synaptophysin (1:1000, Proteintech, Chicago, IL, USA), FKBP51 (1:1000, Proteintech, Chicago, IL, USA), Lamin B1 (1:1000, Proteintech, Chicago, IL, USA), Progesterone Receptor (1:1000, Bioworld, St. Louis, MO, USA), and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5000, Proteintech, Chicago, IL, USA). .. After being washed in TBST 4 times, protein bands were detected by enhanced chemiluminescence and Image J software (Version 1.43, NIH, Bethesda, MD, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA). .. Enzyme-linked immunosorbent assay (ELISA) kits for α-SMA, iNOS, Arg-1, MMP13 and hyaluronic acid (HA) were obtained from CUSABIO (Wuhan, China), and cytokine kits were obtained from R & D Systems (Minneapolis, MN, USA).

    Recombinant:

    Article Title: Inducible Expression of both ermB and ermT Conferred High Macrolide Resistance in Streptococcus gallolyticus subsp. pasteurianus Isolates in China
    Article Snippet: The ErmB and ErmT proteins were detected using in-house mouse serum (1:2000) raised against recombinant ErmB or ErmT protein. .. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected using monoclonal antibody against human GAPDH (1:5000) from Proteintech (Wuhan, China).

    Radio Immunoprecipitation:

    Article Title: Structural Basis for the Inhibition of Host Gene Expression by Porcine Epidemic Diarrhea Virus nsp1
    Article Snippet: The cells were harvested by adding radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime), and the extracts were prepared in SDS-PAGE sample buffer. .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was detected with an anti-GAPDH monoclonal Ab (MAb) (ProteinTech) to confirm equal protein loading.

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Radio-immunoprecipitation assay lysis buffer and nitrocellulose membranes were obtained from Millipore (Billerica, MA, USA). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    FLAG-tag:

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Puromycin (PM), penicillin, streptomycin, phorbol 12-myristate 13-acetate, the hematoxylin and eosin (HE) staining kit, the Masson trichrome staining kit, ionomycin, transforming growth factor beta 1 (TGF-β1) antibody and flag-tag antibody were purchased from Sigma (St. Louis, MO, USA). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    Staining:

    Article Title: Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica
    Article Snippet: Puromycin (PM), penicillin, streptomycin, phorbol 12-myristate 13-acetate, the hematoxylin and eosin (HE) staining kit, the Masson trichrome staining kit, ionomycin, transforming growth factor beta 1 (TGF-β1) antibody and flag-tag antibody were purchased from Sigma (St. Louis, MO, USA). .. Antibodies against iNOS, Arg-1, matrix metalloproteinase 13 (MMP13), α-smooth muscle actin (α-SMA), type 1 collagen (Col I) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for western blotting were purchased from Proteintech (Chicago, IL, USA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Proteintech anti bax
    Effects of H 2 S on the protein expression of <t>Bax,</t> <t>Bcl-2,</t> Caspase-3, and Cleaved Caspase-3 in the kidney of CRF rats were measured. (a) The expression levels of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 were detected by Western blot. β-actin was used as an internal control. Bar graphs showed the quantification of Bax (b), Bcl-2 (c), Caspase-3 (d), and Cleaved Caspase-3 (e). Values were presented as mean ± SEM (n = 3); * P
    Anti Bax, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax/product/Proteintech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bax - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    99
    Proteintech mouse gapdh monoclonal
    <t>CCND2</t> and SERBP1 expression in the HMGA2-IMP2 pathway. (A, B) IMP2 binding sites in the CCND2 and SERBP1 genes. IMP2 bound to CCND2 and SERBP1 mRNAs. (C) RIP experiments in HEK293 cells. CCND2 and SERBP1 proteins were pulled down using anti-IMP2 antibodies. IgG and glyceraldehyde 3-phosphate dehydrogenase <t>(GAPDH)</t> were used as negative controls. (D) Knockdown of HMGA2 in KGN cells reduced the CCND2 and SERBP1 protein levels. (E) Knockdown of IMP2 reduced the CCND2 and SERBP1 protein levels in KGN cells. (F, G) Luciferase assays of IMP2 and downstream genes (F, CCND2 ; G, SERBP1 ) in KGN cells. In both cases, a dose-dependent increase in luciferase activity of CCND2 and SERBP1 was observed as IMP2 expression increased. * P
    Mouse Gapdh Monoclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse gapdh monoclonal/product/Proteintech
    Average 99 stars, based on 221 article reviews
    Price from $9.99 to $1999.99
    mouse gapdh monoclonal - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effects of H 2 S on the protein expression of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 in the kidney of CRF rats were measured. (a) The expression levels of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 were detected by Western blot. β-actin was used as an internal control. Bar graphs showed the quantification of Bax (b), Bcl-2 (c), Caspase-3 (d), and Cleaved Caspase-3 (e). Values were presented as mean ± SEM (n = 3); * P

    Journal: Scientific Reports

    Article Title: Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways

    doi: 10.1038/s41598-017-00557-2

    Figure Lengend Snippet: Effects of H 2 S on the protein expression of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 in the kidney of CRF rats were measured. (a) The expression levels of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 were detected by Western blot. β-actin was used as an internal control. Bar graphs showed the quantification of Bax (b), Bcl-2 (c), Caspase-3 (d), and Cleaved Caspase-3 (e). Values were presented as mean ± SEM (n = 3); * P

    Article Snippet: Anti-Bax, anti-Bcl-2, anti-Caspase-3, anti-Cleaved Caspase-3, and anti-β-actin antibodies were purchased from ProteinTech (Chicago, IL, USA).

    Techniques: Expressing, Western Blot

    Bcl-2 overexpression inhibits the activation of the mitochondrial apoptosis pathway by cisplatin in SKOV3 cells. (A and B) Pc-SKOV3 and Bcl-2-SKOV3 cells were treated with 6 µg/ml cisplatin for 6 h and then stained with MitoPotential dye and 7-AAD to assess the Δψm by flow cytometry. (C and D) Western blot analysis of cytochrome c , Bcl-2, Bax, cleaved caspase-9 and cleaved caspase-3 levels in pc-SKOV3 and Bcl-2-SKOV3 cells after cisplatin treatment. The results are the mean ± SD of three independent experiments. *P

    Journal: Oncology Reports

    Article Title: Bcl-2 overexpression reduces cisplatin cytotoxicity by decreasing ER-mitochondrial Ca2+ signaling in SKOV3 cells

    doi: 10.3892/or.2017.6164

    Figure Lengend Snippet: Bcl-2 overexpression inhibits the activation of the mitochondrial apoptosis pathway by cisplatin in SKOV3 cells. (A and B) Pc-SKOV3 and Bcl-2-SKOV3 cells were treated with 6 µg/ml cisplatin for 6 h and then stained with MitoPotential dye and 7-AAD to assess the Δψm by flow cytometry. (C and D) Western blot analysis of cytochrome c , Bcl-2, Bax, cleaved caspase-9 and cleaved caspase-3 levels in pc-SKOV3 and Bcl-2-SKOV3 cells after cisplatin treatment. The results are the mean ± SD of three independent experiments. *P

    Article Snippet: Anti-β-actin (60008–1-Ig; murine Ab; 1:2,000 dilution), anti-Bax (50599–2-Ig; rabbit Ab; 1:2,000 dilution), anti-Bcl-2 (12789–1-AP; rabbit Ab; 1:1,000 dilution), anti-cytochrome c (cyto c ) (10993–1-AP; rabbit Ab; 1:1,000 dilution), anti-Grp78/BIP (11587–1-AP; rabbit Ab; 1:1,000 dilution), peroxidase-conjugated AffiniPure goat anti-mouse IgG (H+L) (SA00001-1; goat Ab; 1:2,000 dilution) and peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H+L) (SA00001-2; goat Ab; 1:2,000 dilution) Abs were purchased from ProteinTech Group, Inc. (Chicago, IL, USA).

    Techniques: Over Expression, Activation Assay, Staining, Flow Cytometry, Cytometry, Western Blot

    The in vivo antitumor activity of LNPS@siBcl-2 on tumor-bearing nude mice (Panel A ) is the change in tumor volume. (Panel B ) is the body weight change during treatment. (Panel C ) is the Bcl-2, BAX and Caspase-3 expression in tumor tissue after tumor-bearing mice were treated with LNPS@Cy5-siBcl-2. (Panel D ) is the statistic result of panel C. (Panel E ) is representative H E staining picture of major organs and tumor tissue. Data are expressed as the mean ± SD, n = 5. ** P

    Journal: Oncotarget

    Article Title: Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery

    doi: 10.18632/oncotarget.17484

    Figure Lengend Snippet: The in vivo antitumor activity of LNPS@siBcl-2 on tumor-bearing nude mice (Panel A ) is the change in tumor volume. (Panel B ) is the body weight change during treatment. (Panel C ) is the Bcl-2, BAX and Caspase-3 expression in tumor tissue after tumor-bearing mice were treated with LNPS@Cy5-siBcl-2. (Panel D ) is the statistic result of panel C. (Panel E ) is representative H E staining picture of major organs and tumor tissue. Data are expressed as the mean ± SD, n = 5. ** P

    Article Snippet: Anti-β-actin antibody, anti-BAX antibody, anti-Caspase-3 and anti-rabbit IgG H & L (HRP) were purchased from Proteintech (Chicago, USA). siBcl-2 was purchased from Guang Zhou Ribobio Co.,Ltd. (Guangzhou, Guangdong Province, China), and the sequences were as follows: target sense, GGATGACTGAGTACCTGAA.

    Techniques: In Vivo, Activity Assay, Mouse Assay, Expressing, Staining

    Effect of the medium pH on the zeta potential of LNPS@siBcl-2 (panel A ), and gene silencing effect of LNPS@siBcl-2 on A549 cells. (Panel B ) is the cytotoxicity of LNPS@siBcl-2 on A549 cells. (Panel C ) is the Western Blot analysis of Bcl-2, BAX and Caspase-3 on A549 cells. (Panel D ) is the statistic result of protein expression. Data are expressed as the mean ± SD, n = 3. * p

    Journal: Oncotarget

    Article Title: Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery

    doi: 10.18632/oncotarget.17484

    Figure Lengend Snippet: Effect of the medium pH on the zeta potential of LNPS@siBcl-2 (panel A ), and gene silencing effect of LNPS@siBcl-2 on A549 cells. (Panel B ) is the cytotoxicity of LNPS@siBcl-2 on A549 cells. (Panel C ) is the Western Blot analysis of Bcl-2, BAX and Caspase-3 on A549 cells. (Panel D ) is the statistic result of protein expression. Data are expressed as the mean ± SD, n = 3. * p

    Article Snippet: Anti-β-actin antibody, anti-BAX antibody, anti-Caspase-3 and anti-rabbit IgG H & L (HRP) were purchased from Proteintech (Chicago, USA). siBcl-2 was purchased from Guang Zhou Ribobio Co.,Ltd. (Guangzhou, Guangdong Province, China), and the sequences were as follows: target sense, GGATGACTGAGTACCTGAA.

    Techniques: Western Blot, Expressing

    CCND2 and SERBP1 expression in the HMGA2-IMP2 pathway. (A, B) IMP2 binding sites in the CCND2 and SERBP1 genes. IMP2 bound to CCND2 and SERBP1 mRNAs. (C) RIP experiments in HEK293 cells. CCND2 and SERBP1 proteins were pulled down using anti-IMP2 antibodies. IgG and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as negative controls. (D) Knockdown of HMGA2 in KGN cells reduced the CCND2 and SERBP1 protein levels. (E) Knockdown of IMP2 reduced the CCND2 and SERBP1 protein levels in KGN cells. (F, G) Luciferase assays of IMP2 and downstream genes (F, CCND2 ; G, SERBP1 ) in KGN cells. In both cases, a dose-dependent increase in luciferase activity of CCND2 and SERBP1 was observed as IMP2 expression increased. * P

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: The HMGA2-IMP2 Pathway Promotes Granulosa Cell Proliferation in Polycystic Ovary Syndrome

    doi: 10.1210/jc.2018-00544

    Figure Lengend Snippet: CCND2 and SERBP1 expression in the HMGA2-IMP2 pathway. (A, B) IMP2 binding sites in the CCND2 and SERBP1 genes. IMP2 bound to CCND2 and SERBP1 mRNAs. (C) RIP experiments in HEK293 cells. CCND2 and SERBP1 proteins were pulled down using anti-IMP2 antibodies. IgG and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used as negative controls. (D) Knockdown of HMGA2 in KGN cells reduced the CCND2 and SERBP1 protein levels. (E) Knockdown of IMP2 reduced the CCND2 and SERBP1 protein levels in KGN cells. (F, G) Luciferase assays of IMP2 and downstream genes (F, CCND2 ; G, SERBP1 ) in KGN cells. In both cases, a dose-dependent increase in luciferase activity of CCND2 and SERBP1 was observed as IMP2 expression increased. * P

    Article Snippet: The primary antibodies for immunoblotting included anti-HMGA2 antibody (ab184616; Abcam, Cambridge, United Kingdom), anti-IMP2 antibody (14672; Cell Signaling, Danvers, MA), anti–cyclin D2 (CCND2) antibody (3741; Cell Signaling, Danvers, MA), anti–glyceraldehyde 3-phosphate dehydrogenase antibody (60004-1-Ig; Proteintech, Wuhan, China), and anti- SERPINE1 mRNA binding protein 1 (SERBP1) antibody (10729-1-AP; Proteintech, Wuhan, China).

    Techniques: Expressing, Binding Assay, Luciferase, Activity Assay

    CCND2 and SERBP1 mRNA and protein expression in control and PCOS GCs. (A, B) CCND2 mRNA and protein expression was higher in PCOS GCs than in control cells. Data were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). ** P

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: The HMGA2-IMP2 Pathway Promotes Granulosa Cell Proliferation in Polycystic Ovary Syndrome

    doi: 10.1210/jc.2018-00544

    Figure Lengend Snippet: CCND2 and SERBP1 mRNA and protein expression in control and PCOS GCs. (A, B) CCND2 mRNA and protein expression was higher in PCOS GCs than in control cells. Data were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). ** P

    Article Snippet: The primary antibodies for immunoblotting included anti-HMGA2 antibody (ab184616; Abcam, Cambridge, United Kingdom), anti-IMP2 antibody (14672; Cell Signaling, Danvers, MA), anti–cyclin D2 (CCND2) antibody (3741; Cell Signaling, Danvers, MA), anti–glyceraldehyde 3-phosphate dehydrogenase antibody (60004-1-Ig; Proteintech, Wuhan, China), and anti- SERPINE1 mRNA binding protein 1 (SERBP1) antibody (10729-1-AP; Proteintech, Wuhan, China).

    Techniques: Expressing