glyceraldehyde 3 phosphate dehydrogenase gapdh  (Proteintech)

 
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    Structured Review

    Proteintech glyceraldehyde 3 phosphate dehydrogenase gapdh
    Representative western blot analyses of four altered HFF proteins and validation of the expression of upregulated protein WAP four-disulfide core domain protein 2 (WFDC2) by ELISA.  a  The expressions of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), Prostate-specific antigen (KLK3), Fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were confirmed by western blot. Asterisk denoted a statistically significant difference between the two groups;  a  WFDC2 concentration in the follicular fluid samples of 25 normal weight women and 25 overweight status women. ROC curve of WFDC2 distinguished the normal weight and overweight status women
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Proteintech
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-09
    92/100 stars

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    1) Product Images from "Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality"

    Article Title: Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality

    Journal: Clinical Proteomics

    doi: 10.1186/s12014-020-09286-7

    Representative western blot analyses of four altered HFF proteins and validation of the expression of upregulated protein WAP four-disulfide core domain protein 2 (WFDC2) by ELISA.  a  The expressions of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), Prostate-specific antigen (KLK3), Fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were confirmed by western blot. Asterisk denoted a statistically significant difference between the two groups;  a  WFDC2 concentration in the follicular fluid samples of 25 normal weight women and 25 overweight status women. ROC curve of WFDC2 distinguished the normal weight and overweight status women
    Figure Legend Snippet: Representative western blot analyses of four altered HFF proteins and validation of the expression of upregulated protein WAP four-disulfide core domain protein 2 (WFDC2) by ELISA. a The expressions of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), Prostate-specific antigen (KLK3), Fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were confirmed by western blot. Asterisk denoted a statistically significant difference between the two groups; a WFDC2 concentration in the follicular fluid samples of 25 normal weight women and 25 overweight status women. ROC curve of WFDC2 distinguished the normal weight and overweight status women

    Techniques Used: Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay

    Related Articles

    Electrophoresis:

    Article Title: Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction
    Article Snippet: .. Equivalent amounts of protein were separated by electrophoresis on a 10% SDS-PAGE gel; the proteins were then transferred to polyvinylidene fluoride membrane (Millipore) and incubated at 4°C overnight with antibodies against hnRNPM (Abcam ab177957), hnRNPC1+C2 (Abcam, ab10294), TRMT1(Abcam, ab138831), Glyceraldehyde 3-phosphate dehydrogenase/GAPDH (ZSGB-BIO, TA-08), RCAN2 (Proteintech, 12900-1-AP, Illinois, USA), and PPIL4 (Proteintech, 12538-1-AP). .. Peroxidase-conjugated AffiniPure Goat anti-Rabbit IgG (ZSGB-BIO, ZB-2301, 1/5000) and anti-mouse IgG (ZSGB, ZB-2305, 1/5000) were used and SuperSignal® West Pico Trial Kit (Thermo Fisher Scientific) was applied for protein detection.

    Incubation:

    Article Title: Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction
    Article Snippet: .. Equivalent amounts of protein were separated by electrophoresis on a 10% SDS-PAGE gel; the proteins were then transferred to polyvinylidene fluoride membrane (Millipore) and incubated at 4°C overnight with antibodies against hnRNPM (Abcam ab177957), hnRNPC1+C2 (Abcam, ab10294), TRMT1(Abcam, ab138831), Glyceraldehyde 3-phosphate dehydrogenase/GAPDH (ZSGB-BIO, TA-08), RCAN2 (Proteintech, 12900-1-AP, Illinois, USA), and PPIL4 (Proteintech, 12538-1-AP). .. Peroxidase-conjugated AffiniPure Goat anti-Rabbit IgG (ZSGB-BIO, ZB-2301, 1/5000) and anti-mouse IgG (ZSGB, ZB-2305, 1/5000) were used and SuperSignal® West Pico Trial Kit (Thermo Fisher Scientific) was applied for protein detection.

    Article Title: Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality
    Article Snippet: .. Further, 5% (w/v) skimmed milk was used to block the above membrane at 37 °C for 1 h, and the primary antibodies (WAP four-disulfide core domain protein 2 (WFDC2), ab109298, Abcam, Cambridge, USA; lactotransferrin (LTF), ab109000, Abcam, Cambridge, USA; Prostate-specific antigen (KLK3), ab76113, Abcam, Cambridge, USA; Fibronectin (FN1), ab32419, Abcam, Cambridge, USA; glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 60004-1-Ig, Proteintech Group Chicago, USA) were respectively added for incubation at 4 °C overnight. .. The resultant membranes were washed with TBST for three times, and then incubated with horse-radish peroxidase-conjugated secondary antibody (diluted 1:5000, Zhong-Shan Biotechnology, Beijing, China) at room temperature for 1 h. The enhanced chemiluminescence detection reagents (Pierce, Rockford, IL, USA) was used to visualize the immunoreactive proteins.

    SDS Page:

    Article Title: Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction
    Article Snippet: .. Equivalent amounts of protein were separated by electrophoresis on a 10% SDS-PAGE gel; the proteins were then transferred to polyvinylidene fluoride membrane (Millipore) and incubated at 4°C overnight with antibodies against hnRNPM (Abcam ab177957), hnRNPC1+C2 (Abcam, ab10294), TRMT1(Abcam, ab138831), Glyceraldehyde 3-phosphate dehydrogenase/GAPDH (ZSGB-BIO, TA-08), RCAN2 (Proteintech, 12900-1-AP, Illinois, USA), and PPIL4 (Proteintech, 12538-1-AP). .. Peroxidase-conjugated AffiniPure Goat anti-Rabbit IgG (ZSGB-BIO, ZB-2301, 1/5000) and anti-mouse IgG (ZSGB, ZB-2305, 1/5000) were used and SuperSignal® West Pico Trial Kit (Thermo Fisher Scientific) was applied for protein detection.

    Expressing:

    Article Title: Structural and Biological Basis of Alphacoronavirus nsp1 Associated with Host Proliferation and Immune Evasion
    Article Snippet: .. The expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was assessed with an anti-GAPDH monoclonal Ab (mAb; ProteinTech, Wuhan, China) to confirm equal protein loading. ..

    Blocking Assay:

    Article Title: Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality
    Article Snippet: .. Further, 5% (w/v) skimmed milk was used to block the above membrane at 37 °C for 1 h, and the primary antibodies (WAP four-disulfide core domain protein 2 (WFDC2), ab109298, Abcam, Cambridge, USA; lactotransferrin (LTF), ab109000, Abcam, Cambridge, USA; Prostate-specific antigen (KLK3), ab76113, Abcam, Cambridge, USA; Fibronectin (FN1), ab32419, Abcam, Cambridge, USA; glyceraldehyde 3-phosphate dehydrogenase (GAPDH), 60004-1-Ig, Proteintech Group Chicago, USA) were respectively added for incubation at 4 °C overnight. .. The resultant membranes were washed with TBST for three times, and then incubated with horse-radish peroxidase-conjugated secondary antibody (diluted 1:5000, Zhong-Shan Biotechnology, Beijing, China) at room temperature for 1 h. The enhanced chemiluminescence detection reagents (Pierce, Rockford, IL, USA) was used to visualize the immunoreactive proteins.

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    Proteintech anti bax
    Effects of H 2 S on the protein expression of <t>Bax,</t> <t>Bcl-2,</t> Caspase-3, and Cleaved Caspase-3 in the kidney of CRF rats were measured. (a) The expression levels of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 were detected by Western blot. β-actin was used as an internal control. Bar graphs showed the quantification of Bax (b), Bcl-2 (c), Caspase-3 (d), and Cleaved Caspase-3 (e). Values were presented as mean ± SEM (n = 3); * P
    Anti Bax, supplied by Proteintech, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax/product/Proteintech
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    anti bax - by Bioz Stars, 2020-09
    99/100 stars
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    Effects of H 2 S on the protein expression of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 in the kidney of CRF rats were measured. (a) The expression levels of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 were detected by Western blot. β-actin was used as an internal control. Bar graphs showed the quantification of Bax (b), Bcl-2 (c), Caspase-3 (d), and Cleaved Caspase-3 (e). Values were presented as mean ± SEM (n = 3); * P

    Journal: Scientific Reports

    Article Title: Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF-κB signaling pathways

    doi: 10.1038/s41598-017-00557-2

    Figure Lengend Snippet: Effects of H 2 S on the protein expression of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 in the kidney of CRF rats were measured. (a) The expression levels of Bax, Bcl-2, Caspase-3, and Cleaved Caspase-3 were detected by Western blot. β-actin was used as an internal control. Bar graphs showed the quantification of Bax (b), Bcl-2 (c), Caspase-3 (d), and Cleaved Caspase-3 (e). Values were presented as mean ± SEM (n = 3); * P

    Article Snippet: Anti-Bax, anti-Bcl-2, anti-Caspase-3, anti-Cleaved Caspase-3, and anti-β-actin antibodies were purchased from ProteinTech (Chicago, IL, USA).

    Techniques: Expressing, Western Blot

    Bcl-2 overexpression inhibits the activation of the mitochondrial apoptosis pathway by cisplatin in SKOV3 cells. (A and B) Pc-SKOV3 and Bcl-2-SKOV3 cells were treated with 6 µg/ml cisplatin for 6 h and then stained with MitoPotential dye and 7-AAD to assess the Δψm by flow cytometry. (C and D) Western blot analysis of cytochrome c , Bcl-2, Bax, cleaved caspase-9 and cleaved caspase-3 levels in pc-SKOV3 and Bcl-2-SKOV3 cells after cisplatin treatment. The results are the mean ± SD of three independent experiments. *P

    Journal: Oncology Reports

    Article Title: Bcl-2 overexpression reduces cisplatin cytotoxicity by decreasing ER-mitochondrial Ca2+ signaling in SKOV3 cells

    doi: 10.3892/or.2017.6164

    Figure Lengend Snippet: Bcl-2 overexpression inhibits the activation of the mitochondrial apoptosis pathway by cisplatin in SKOV3 cells. (A and B) Pc-SKOV3 and Bcl-2-SKOV3 cells were treated with 6 µg/ml cisplatin for 6 h and then stained with MitoPotential dye and 7-AAD to assess the Δψm by flow cytometry. (C and D) Western blot analysis of cytochrome c , Bcl-2, Bax, cleaved caspase-9 and cleaved caspase-3 levels in pc-SKOV3 and Bcl-2-SKOV3 cells after cisplatin treatment. The results are the mean ± SD of three independent experiments. *P

    Article Snippet: Anti-β-actin (60008–1-Ig; murine Ab; 1:2,000 dilution), anti-Bax (50599–2-Ig; rabbit Ab; 1:2,000 dilution), anti-Bcl-2 (12789–1-AP; rabbit Ab; 1:1,000 dilution), anti-cytochrome c (cyto c ) (10993–1-AP; rabbit Ab; 1:1,000 dilution), anti-Grp78/BIP (11587–1-AP; rabbit Ab; 1:1,000 dilution), peroxidase-conjugated AffiniPure goat anti-mouse IgG (H+L) (SA00001-1; goat Ab; 1:2,000 dilution) and peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H+L) (SA00001-2; goat Ab; 1:2,000 dilution) Abs were purchased from ProteinTech Group, Inc. (Chicago, IL, USA).

    Techniques: Over Expression, Activation Assay, Staining, Flow Cytometry, Cytometry, Western Blot

    Schematic representation illustrating the potential mechanisms behind the protective effects of mild hypothermia against hepatic IR injury. Pretreatment with mild hypothermia promotes the homodimerization of leptin (LEP) and leptin receptor (LEPR), leading to the phosphorylation of JAK2/STAT3 and hence the activation of the signal transduction pathway. Activation of the JAK2/STAT3 pathway leads to the upregulation of the expression of CPT1, which is the rate-limiting enzyme of FAO and thereby accelerates hepatic FAO and ATP production. Sufficient ATP levels will alleviate IR-induced mitochondrial injury, inhibit the release of ROS, and reduce lipid peroxidation, thus attenuating oxidative stress. Furthermore, activation of the JAK2/STAT3 pathway promotes the expression of BCL-2 and inhibits the expression of BAX, thereby reducing hepatocyte apoptosis.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Mild Hypothermia Attenuates Hepatic Ischemia–Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation

    doi: 10.1155/2020/5849794

    Figure Lengend Snippet: Schematic representation illustrating the potential mechanisms behind the protective effects of mild hypothermia against hepatic IR injury. Pretreatment with mild hypothermia promotes the homodimerization of leptin (LEP) and leptin receptor (LEPR), leading to the phosphorylation of JAK2/STAT3 and hence the activation of the signal transduction pathway. Activation of the JAK2/STAT3 pathway leads to the upregulation of the expression of CPT1, which is the rate-limiting enzyme of FAO and thereby accelerates hepatic FAO and ATP production. Sufficient ATP levels will alleviate IR-induced mitochondrial injury, inhibit the release of ROS, and reduce lipid peroxidation, thus attenuating oxidative stress. Furthermore, activation of the JAK2/STAT3 pathway promotes the expression of BCL-2 and inhibits the expression of BAX, thereby reducing hepatocyte apoptosis.

    Article Snippet: The primary antibodies used in these experiments were the following: rabbit anti-JAK2 (1 : 750, Proteintech, Manchester, UK), rabbit anti-p-JAK2 (phosphorylated JAK2 at Tyr1007/1008) (1 : 1000, Cell Signaling, Danvers, MA, USA), rabbit anti-STAT3 (1 : 750, Proteintech, Manchester, UK), mouse anti-p-STAT3 (phosphorylated STAT3 at Tyr705) (1 : 1000, Cell Signaling, Danvers, MA, USA), rabbit anti-ACSL1 (1 : 750, Proteintech, Manchester, UK), rabbit anti-CPT1a (1 : 1000, Proteintech, Manchester, UK), rabbit anti-ACADVL (1 : 750, Proteintech, Manchester, UK), rabbit anti-HADHA (1 : 1000, Proteintech, Manchester, UK), rabbit anti-HMGCS1 (1 : 750, Proteintech, Manchester, UK), mouse anti-BCL-2 (1 : 1000, Cell Signaling, Danvers, MA, USA), rabbit anti-BAX (1 : 750, Proteintech, Manchester, UK), rabbit anti-PFKM (1 : 1000, Proteintech, Manchester, UK), rabbit anti-IDH2 (1 : 1000, Proteintech, Manchester, UK), and rabbit anti-CS (1 : 1000, Proteintech, Manchester, UK).

    Techniques: Activation Assay, Transduction, Expressing

    Mild hypothermia maintains the activation of the JAK2/STAT3 pathway after hepatic IR injury. (a) Representative blots of JAK2, STAT3, and important markers of apoptosis. (b) The phosphorylation levels of JAK2 at Tyr1007/1008. (c) The phosphorylation levels of STAT3 at Tyr705. (d) The ratio of BCL-2/BAX.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Mild Hypothermia Attenuates Hepatic Ischemia–Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation

    doi: 10.1155/2020/5849794

    Figure Lengend Snippet: Mild hypothermia maintains the activation of the JAK2/STAT3 pathway after hepatic IR injury. (a) Representative blots of JAK2, STAT3, and important markers of apoptosis. (b) The phosphorylation levels of JAK2 at Tyr1007/1008. (c) The phosphorylation levels of STAT3 at Tyr705. (d) The ratio of BCL-2/BAX.

    Article Snippet: The primary antibodies used in these experiments were the following: rabbit anti-JAK2 (1 : 750, Proteintech, Manchester, UK), rabbit anti-p-JAK2 (phosphorylated JAK2 at Tyr1007/1008) (1 : 1000, Cell Signaling, Danvers, MA, USA), rabbit anti-STAT3 (1 : 750, Proteintech, Manchester, UK), mouse anti-p-STAT3 (phosphorylated STAT3 at Tyr705) (1 : 1000, Cell Signaling, Danvers, MA, USA), rabbit anti-ACSL1 (1 : 750, Proteintech, Manchester, UK), rabbit anti-CPT1a (1 : 1000, Proteintech, Manchester, UK), rabbit anti-ACADVL (1 : 750, Proteintech, Manchester, UK), rabbit anti-HADHA (1 : 1000, Proteintech, Manchester, UK), rabbit anti-HMGCS1 (1 : 750, Proteintech, Manchester, UK), mouse anti-BCL-2 (1 : 1000, Cell Signaling, Danvers, MA, USA), rabbit anti-BAX (1 : 750, Proteintech, Manchester, UK), rabbit anti-PFKM (1 : 1000, Proteintech, Manchester, UK), rabbit anti-IDH2 (1 : 1000, Proteintech, Manchester, UK), and rabbit anti-CS (1 : 1000, Proteintech, Manchester, UK).

    Techniques: Activation Assay

    The effect of alpha lipoic acid on the expression of key apoptosis-related modulators in the testes of chickens exposed to heat stress. Western blotting analysis of the expression of apoptosis-related proteins, ( A ) Bax, ( C ) Bcl-2, and ( E ) caspase 3. ( B ) Bax, ( D ) Bcl-2, and ( F ) caspase 3 protein expression levels were normalized to β-actin levels. The statistical analysis results are shown as bar graphs. The data are presented as the mean ± SE of three independent experiments. Note: * p

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Oxidative Stress and Endoplasmic Reticulum Stress Are Involved in the Protective Effect of Alpha Lipoic Acid Against Heat Damage in Chicken Testes

    doi: 10.3390/ani10030384

    Figure Lengend Snippet: The effect of alpha lipoic acid on the expression of key apoptosis-related modulators in the testes of chickens exposed to heat stress. Western blotting analysis of the expression of apoptosis-related proteins, ( A ) Bax, ( C ) Bcl-2, and ( E ) caspase 3. ( B ) Bax, ( D ) Bcl-2, and ( F ) caspase 3 protein expression levels were normalized to β-actin levels. The statistical analysis results are shown as bar graphs. The data are presented as the mean ± SE of three independent experiments. Note: * p

    Article Snippet: The membranes were completely immersed in blocking buffer (P0252-100, Beyotime Biotechnology, Shanghai, China) for 90 min at room temperature and were then incubated with anti-glucose-regulated protein 78 (GRP78) (1:1,000; Proteintech, Wuhan, China), anti-CHOP (1:1000; Proteintech), anti-Bax (1:1,000; Proteintech), anti-Bcl-2 (1:1000; Proteintech), or anti-caspase 3 (1:1000; Proteintech) primary antibodies at 4 °C for 10 h. After incubation, the PVDF membranes were washed twice with TBST (Tris-buffered saline containing 0.1% Tween 20), and then incubated with HRP-conjugated secondary antibody (1:5000; Beyotime Biotechnology) for 1.5 h at 37 °C.

    Techniques: Expressing, Western Blot