glyceraldehyde 3 phosphate dehydrogenase gapdh  (Millipore)


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    Structured Review

    Millipore glyceraldehyde 3 phosphate dehydrogenase gapdh
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101
    Article Snippet: RFP-LC3, HA-TSG101ΔUEV and MGRN1 (SRAP) were generated using standard cloning and mutation techniques. .. Antibodies were from the following sources: CD63 (BD Pharmingen, #556019, San Jose, CA, USA), LAMP2 (H4B4, Hybridoma Technology, Iowa City, IA, USA), Beclin1 (Novus Biologicals, #NB500-249, Littleton, CO, USA), LC3 (Novus Biologicals, #NB100-2220), p62/ SQSTM1 (Thermo Scientific, #PA5-20839, Rockford, IL, USA), β- catenin (Cell Signaling Technology, #9562, Danvers, MA, USA), Monoclonal anti GAPDH clone GAPDH-71.1 (Sigma-Aldrich, #G8795, St. Louis, MO, USA), EGFR (A10) (Santa Cruz Biotechnology, Inc., #sc-373746, Dallas, TX, USA), Rab7 (Abcam, #ab50533, Cambridge, UK), β -tubulin (Abcam, #ab7792), TSG101(Abcam, #ab83), Ub (Sigma-Aldrich, #U0508, Anti-Cathepsin D (CTD-19, Abcam, #ab6313)).

    Centrifugation:

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: This cell suspension was pipetted up and down to lyse fibers, The homogenates were subjected to centrifugation at 10,000 rpm for 10 min at 4°C. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: The soluble cytosolic fraction was separated from the membrane-enriched fraction by centrifugation at 19,000 g for 10 min at 4°C. .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374).

    Blocking Assay:

    Article Title: Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators
    Article Snippet: .. Each section was probed separately with antibodies to each of the following proteins in blocking buffer: MCL1 (Cell Signaling Technologies 5453) (D35A5) rabbit monoclonal diluted 1:500, BCLxL (Cell Signaling Technologies 2764) (54H6) rabbit monoclonal diluted 1:500, BCL2 (Cell Signaling Technologies D55G8) (4223) rabbit monoclonal diluted 1:500, BCL2L2/BCLw (R & D Systems AF824) rabbit polyclonal diluted 1:500, Cleaved Parp1 (Cell Signaling Technologies 5625) (D64P10) rabbit monoclonal diluted 1:500, Tubulin (Sigma T6199) mouse monoclonal at 50 ng ml−1 , and GAPDH (Sigma G8795) mouse monoclonal at 100 ng ml−1 . ..

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374). .. After washing, the membranes were developed using corresponding anti-mouse or anti-rabbit secondary antibodies (Calbiochem).

    Electrophoresis:

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: After separation by electrophoresis, gels were blotted overnight at 4°C onto Polyvinylidene fluoride (PVDF) membranes. .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374).

    Quantitation Assay:

    Article Title: RARβ2-dependent signaling represses neuronal differentiation in mouse ES cells
    Article Snippet: The blots were probed with anti-GAPDH (SAB2108266, 1:500, Sigma-Aldrich), anti-phospho-p38 MAPK (44-684G, 1:1000, Invitrogen/Thermo Fisher Scientific), and anti-phospho-PI3K (4292, 1:1000, Cell Signaling Technology). .. Quantitation of the bands was performed by densitometry tracing using ImageJ and/or the AlphaEase™ software.

    Expressing:

    Article Title: DNA methylation and histone modifications regulate SOX11 expression in lymphoid and solid cancer cells
    Article Snippet: .. Protein expression were assessed using the following antibodies: SOX11 monoclonal antibody [ ], mouse anti GAPDH antibody (G8795, Sigma-Aldrich, St Louis, MO, USA) and EZH2 monoclonal antibody (Clone 11/EZH2, BD Transduction Laboratories, Franklin Lakes, NJ, USA). .. A HRP-labeled anti mouse antibody (P0260, DAKO, Glostrup, Denmark) was used for detection.

    BIA-KA:

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: Protein concentration was determined using the BCA method in a 96 well plate (200 μL total volume for each well) incubating at 37°C in the dark for 30 min. Absorbance at 540 nm was measured by a Packard Spectra Count 96 well plate reader. .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374).

    Western Blot:

    Article Title: RARβ2-dependent signaling represses neuronal differentiation in mouse ES cells
    Article Snippet: Paragraph title: 2.5. Western blotting ... The blots were probed with anti-GAPDH (SAB2108266, 1:500, Sigma-Aldrich), anti-phospho-p38 MAPK (44-684G, 1:1000, Invitrogen/Thermo Fisher Scientific), and anti-phospho-PI3K (4292, 1:1000, Cell Signaling Technology).

    Article Title: Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators
    Article Snippet: Paragraph title: Western blot analysis ... Each section was probed separately with antibodies to each of the following proteins in blocking buffer: MCL1 (Cell Signaling Technologies 5453) (D35A5) rabbit monoclonal diluted 1:500, BCLxL (Cell Signaling Technologies 2764) (54H6) rabbit monoclonal diluted 1:500, BCL2 (Cell Signaling Technologies D55G8) (4223) rabbit monoclonal diluted 1:500, BCL2L2/BCLw (R & D Systems AF824) rabbit polyclonal diluted 1:500, Cleaved Parp1 (Cell Signaling Technologies 5625) (D64P10) rabbit monoclonal diluted 1:500, Tubulin (Sigma T6199) mouse monoclonal at 50 ng ml−1 , and GAPDH (Sigma G8795) mouse monoclonal at 100 ng ml−1 .

    Article Title: DNA methylation and histone modifications regulate SOX11 expression in lymphoid and solid cancer cells
    Article Snippet: Paragraph title: Western blot analysis of SOX11 and EZH2 ... Protein expression were assessed using the following antibodies: SOX11 monoclonal antibody [ ], mouse anti GAPDH antibody (G8795, Sigma-Aldrich, St Louis, MO, USA) and EZH2 monoclonal antibody (Clone 11/EZH2, BD Transduction Laboratories, Franklin Lakes, NJ, USA).

    Article Title: Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Article Snippet: Paragraph title: SDS–PAGE and western blotting ... The following primary antibodies were used: anti-Lig3 (1F3) mouse mAb (GeneTex); anti-GAPDH mouse mAb (Millipore).

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: Paragraph title: Western blotting. ... Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: Paragraph title: Western blotting ... After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374).

    Electron Microscopy:

    Article Title: A high-content EMT screen identifies multiple receptor tyrosine kinase inhibitors with activity on TGFβ receptor
    Article Snippet: Rat anti-E-cadherin antibody was obtained from ThermoFisher Scientific (13-1900), rabbit anti-ZO1 antibody from ThermoFisher Scientific (61-7300), mouse anti-vimentin antibody from Sigma (V2258), mouse anti-SMAD2/3 from BD Biosciences (610842), rabbit anti-p-SMAD3 from Cell Signaling (p-Ser423/425; 9520) and mouse anti-GAPDH from Sigma (G8795). .. DAPI was acquired from Sigma (D9542), recombinant human TGFβ1 protein from R & D Systems (240B-0-10), 16% paraformaldehyd (PFA) from Electron Microscopy Services (15710-S), fatty-acid free BSA from Calbiochem (126575), Trypsin/EDTA from Sigma (T4174), PBS from Gibco (14200-067), and Triton X-100 from Sigma (X-100).

    Protease Inhibitor:

    Article Title: Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators
    Article Snippet: Cell lines were lysed in RIPA buffer (Boston BioProducts) plus protease-inhibitor cocktail (Mini-complete, EDTA-free, Roche) and phosphatase inhibitor PhosSTOP (Roche). .. Each section was probed separately with antibodies to each of the following proteins in blocking buffer: MCL1 (Cell Signaling Technologies 5453) (D35A5) rabbit monoclonal diluted 1:500, BCLxL (Cell Signaling Technologies 2764) (54H6) rabbit monoclonal diluted 1:500, BCL2 (Cell Signaling Technologies D55G8) (4223) rabbit monoclonal diluted 1:500, BCL2L2/BCLw (R & D Systems AF824) rabbit polyclonal diluted 1:500, Cleaved Parp1 (Cell Signaling Technologies 5625) (D64P10) rabbit monoclonal diluted 1:500, Tubulin (Sigma T6199) mouse monoclonal at 50 ng ml−1 , and GAPDH (Sigma G8795) mouse monoclonal at 100 ng ml−1 .

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: Cultured FDB fibers were homogenized with a mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL) supplemented with protease inhibitor cocktail (Complete-Mini EDTA free; Roche Diagnostics) at 4°C for 60 min. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Cell Culture:

    Article Title: microRNA-103 regulates the growth and invasion of endometrial cancer cells through the downregulation of tissue inhibitor of metalloproteinase 3
    Article Snippet: Paragraph title: Cell culture and reagents ... The TIMP-3 antibody was purchased from Abcam (Cambridge, MA, USA), GAPDH antibody from Sigma (Steinheim, Germany) and goat anti-mouse IgG from Bio-Rad (Hercules, CA, USA).

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: Cultured FDB fibers were homogenized with a mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL) supplemented with protease inhibitor cocktail (Complete-Mini EDTA free; Roche Diagnostics) at 4°C for 60 min. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    SDS Page:

    Article Title: Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Article Snippet: Paragraph title: SDS–PAGE and western blotting ... The following primary antibodies were used: anti-Lig3 (1F3) mouse mAb (GeneTex); anti-GAPDH mouse mAb (Millipore).

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: Then, 20 μg of protein samples were fractionated by 4–12% SDS-PAGE at 120 V at 4°C and transferred to PVDF membrane at 22 V overnight at 4°C. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: The pelleted membranes were suspended in TES buffer and separated by SDS-PAGE in a 10% polyacrylamide gel containing 6 M Urea. .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374).

    Generated:

    Article Title: Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101
    Article Snippet: RFP-LC3, HA-TSG101ΔUEV and MGRN1 (SRAP) were generated using standard cloning and mutation techniques. .. Antibodies were from the following sources: CD63 (BD Pharmingen, #556019, San Jose, CA, USA), LAMP2 (H4B4, Hybridoma Technology, Iowa City, IA, USA), Beclin1 (Novus Biologicals, #NB500-249, Littleton, CO, USA), LC3 (Novus Biologicals, #NB100-2220), p62/ SQSTM1 (Thermo Scientific, #PA5-20839, Rockford, IL, USA), β- catenin (Cell Signaling Technology, #9562, Danvers, MA, USA), Monoclonal anti GAPDH clone GAPDH-71.1 (Sigma-Aldrich, #G8795, St. Louis, MO, USA), EGFR (A10) (Santa Cruz Biotechnology, Inc., #sc-373746, Dallas, TX, USA), Rab7 (Abcam, #ab50533, Cambridge, UK), β -tubulin (Abcam, #ab7792), TSG101(Abcam, #ab83), Ub (Sigma-Aldrich, #U0508, Anti-Cathepsin D (CTD-19, Abcam, #ab6313)).

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: The antibody against Parkin phospho-Ser65 (S210D) was generated by injection of the KLH-conjugated phospho-peptide RDLDQQpSIVHIVQR (where pS is phospho-serine) into sheep and was affinity-purified by positive and negative selection against the phospho- and de-phospho-peptides, respectively. .. Anti-human PINK1 rabbit polyclonal (residues 175–250) antibody was obtained from Novus Biologicals; anti-GAPDH mouse monoclonal from Millipore; anti-Parkin mouse monoclonal (Santa Cruz).

    Imaging:

    Article Title: Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Article Snippet: Signals were visualized in a VersaDocTM imaging system (Bio-Rad) and analyzed using the ‘Quantity One’ software (Bio-Rad). .. The following primary antibodies were used: anti-Lig3 (1F3) mouse mAb (GeneTex); anti-GAPDH mouse mAb (Millipore).

    Protein Concentration:

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: Protein concentration was determined using the BCA method in a 96 well plate (200 μL total volume for each well) incubating at 37°C in the dark for 30 min. Absorbance at 540 nm was measured by a Packard Spectra Count 96 well plate reader. .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374).

    Injection:

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: The antibody against Parkin phospho-Ser65 (S210D) was generated by injection of the KLH-conjugated phospho-peptide RDLDQQpSIVHIVQR (where pS is phospho-serine) into sheep and was affinity-purified by positive and negative selection against the phospho- and de-phospho-peptides, respectively. .. Anti-human PINK1 rabbit polyclonal (residues 175–250) antibody was obtained from Novus Biologicals; anti-GAPDH mouse monoclonal from Millipore; anti-Parkin mouse monoclonal (Santa Cruz).

    Recombinant:

    Article Title: A high-content EMT screen identifies multiple receptor tyrosine kinase inhibitors with activity on TGFβ receptor
    Article Snippet: Rat anti-E-cadherin antibody was obtained from ThermoFisher Scientific (13-1900), rabbit anti-ZO1 antibody from ThermoFisher Scientific (61-7300), mouse anti-vimentin antibody from Sigma (V2258), mouse anti-SMAD2/3 from BD Biosciences (610842), rabbit anti-p-SMAD3 from Cell Signaling (p-Ser423/425; 9520) and mouse anti-GAPDH from Sigma (G8795). .. DAPI was acquired from Sigma (D9542), recombinant human TGFβ1 protein from R & D Systems (240B-0-10), 16% paraformaldehyd (PFA) from Electron Microscopy Services (15710-S), fatty-acid free BSA from Calbiochem (126575), Trypsin/EDTA from Sigma (T4174), PBS from Gibco (14200-067), and Triton X-100 from Sigma (X-100).

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: The antibody against total PINK1 (S085D) was raised in sheep against a recombinant GST-PINK1 fragment (residues 175–250) and successively purified by positive and negative selection against recombinant fusion protein and GST, respectively. .. Anti-human PINK1 rabbit polyclonal (residues 175–250) antibody was obtained from Novus Biologicals; anti-GAPDH mouse monoclonal from Millipore; anti-Parkin mouse monoclonal (Santa Cruz).

    Mutagenesis:

    Article Title: Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101
    Article Snippet: RFP-LC3, HA-TSG101ΔUEV and MGRN1 (SRAP) were generated using standard cloning and mutation techniques. .. Antibodies were from the following sources: CD63 (BD Pharmingen, #556019, San Jose, CA, USA), LAMP2 (H4B4, Hybridoma Technology, Iowa City, IA, USA), Beclin1 (Novus Biologicals, #NB500-249, Littleton, CO, USA), LC3 (Novus Biologicals, #NB100-2220), p62/ SQSTM1 (Thermo Scientific, #PA5-20839, Rockford, IL, USA), β- catenin (Cell Signaling Technology, #9562, Danvers, MA, USA), Monoclonal anti GAPDH clone GAPDH-71.1 (Sigma-Aldrich, #G8795, St. Louis, MO, USA), EGFR (A10) (Santa Cruz Biotechnology, Inc., #sc-373746, Dallas, TX, USA), Rab7 (Abcam, #ab50533, Cambridge, UK), β -tubulin (Abcam, #ab7792), TSG101(Abcam, #ab83), Ub (Sigma-Aldrich, #U0508, Anti-Cathepsin D (CTD-19, Abcam, #ab6313)).

    Isolation:

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: An independent experiment consisted of cultured isolated fibers from two whole FDB muscles, three to five mice per group. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Immunodetection:

    Article Title: Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Article Snippet: Equal loading and transfer efficiency were monitored by Ponceau-S staining combined with immunodetection of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). .. The following primary antibodies were used: anti-Lig3 (1F3) mouse mAb (GeneTex); anti-GAPDH mouse mAb (Millipore).

    Mouse Assay:

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: An independent experiment consisted of cultured isolated fibers from two whole FDB muscles, three to five mice per group. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Protein Extraction:

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: Cultured FDB fibers were homogenized with a mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL) supplemented with protease inhibitor cocktail (Complete-Mini EDTA free; Roche Diagnostics) at 4°C for 60 min. .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Construct:

    Article Title: Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101
    Article Snippet: Paragraph title: Constructs, antibodies and reagents ... Antibodies were from the following sources: CD63 (BD Pharmingen, #556019, San Jose, CA, USA), LAMP2 (H4B4, Hybridoma Technology, Iowa City, IA, USA), Beclin1 (Novus Biologicals, #NB500-249, Littleton, CO, USA), LC3 (Novus Biologicals, #NB100-2220), p62/ SQSTM1 (Thermo Scientific, #PA5-20839, Rockford, IL, USA), β- catenin (Cell Signaling Technology, #9562, Danvers, MA, USA), Monoclonal anti GAPDH clone GAPDH-71.1 (Sigma-Aldrich, #G8795, St. Louis, MO, USA), EGFR (A10) (Santa Cruz Biotechnology, Inc., #sc-373746, Dallas, TX, USA), Rab7 (Abcam, #ab50533, Cambridge, UK), β -tubulin (Abcam, #ab7792), TSG101(Abcam, #ab83), Ub (Sigma-Aldrich, #U0508, Anti-Cathepsin D (CTD-19, Abcam, #ab6313)).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Sensitivity to splicing modulation of BCL2 family genes defines cancer therapeutic strategies for splicing modulators
    Article Snippet: Lysates were diluted in RIPA buffer with 4× LDS Sample Buffer (Nupage, Thermo Fisher Scientific) and 10× Reducing Reagent (Nupage, Thermo Fisher Scientific) and boiled for 5 min. Twenty-five micrograms of protein were loaded per well in 4–12% Bis-Tris sodium dodecyl sulfate Page gels (Novex, Thermo Fisher Scientific). .. Each section was probed separately with antibodies to each of the following proteins in blocking buffer: MCL1 (Cell Signaling Technologies 5453) (D35A5) rabbit monoclonal diluted 1:500, BCLxL (Cell Signaling Technologies 2764) (54H6) rabbit monoclonal diluted 1:500, BCL2 (Cell Signaling Technologies D55G8) (4223) rabbit monoclonal diluted 1:500, BCL2L2/BCLw (R & D Systems AF824) rabbit polyclonal diluted 1:500, Cleaved Parp1 (Cell Signaling Technologies 5625) (D64P10) rabbit monoclonal diluted 1:500, Tubulin (Sigma T6199) mouse monoclonal at 50 ng ml−1 , and GAPDH (Sigma G8795) mouse monoclonal at 100 ng ml−1 .

    Staining:

    Article Title: Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Article Snippet: Equal loading and transfer efficiency were monitored by Ponceau-S staining combined with immunodetection of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). .. The following primary antibodies were used: anti-Lig3 (1F3) mouse mAb (GeneTex); anti-GAPDH mouse mAb (Millipore).

    IA:

    Article Title: Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101
    Article Snippet: .. Antibodies were from the following sources: CD63 (BD Pharmingen, #556019, San Jose, CA, USA), LAMP2 (H4B4, Hybridoma Technology, Iowa City, IA, USA), Beclin1 (Novus Biologicals, #NB500-249, Littleton, CO, USA), LC3 (Novus Biologicals, #NB100-2220), p62/ SQSTM1 (Thermo Scientific, #PA5-20839, Rockford, IL, USA), β- catenin (Cell Signaling Technology, #9562, Danvers, MA, USA), Monoclonal anti GAPDH clone GAPDH-71.1 (Sigma-Aldrich, #G8795, St. Louis, MO, USA), EGFR (A10) (Santa Cruz Biotechnology, Inc., #sc-373746, Dallas, TX, USA), Rab7 (Abcam, #ab50533, Cambridge, UK), β -tubulin (Abcam, #ab7792), TSG101(Abcam, #ab83), Ub (Sigma-Aldrich, #U0508, Anti-Cathepsin D (CTD-19, Abcam, #ab6313)). ..

    Purification:

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: The antibody against total PINK1 (S085D) was raised in sheep against a recombinant GST-PINK1 fragment (residues 175–250) and successively purified by positive and negative selection against recombinant fusion protein and GST, respectively. .. Anti-human PINK1 rabbit polyclonal (residues 175–250) antibody was obtained from Novus Biologicals; anti-GAPDH mouse monoclonal from Millipore; anti-Parkin mouse monoclonal (Santa Cruz).

    Affinity Purification:

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: The antibody against total Parkin (S966C) was raised in sheep against the recombinant GST-Parkin full-length protein and successively affinity-purified by positive and negative selection against recombinant fusion protein and GST, respectively. .. Anti-human PINK1 rabbit polyclonal (residues 175–250) antibody was obtained from Novus Biologicals; anti-GAPDH mouse monoclonal from Millipore; anti-Parkin mouse monoclonal (Santa Cruz).

    Plasmid Preparation:

    Article Title: Hippo signaling is intrinsically regulated during cell cycle progression by APC/CCdh1
    Article Snippet: Anti-Flag (M2, F3165), anti-GAPDH (SAB1405848), anti-YAP (WH0010413M1), and anti-TAZ (T4077) antibodies were from Sigma-Aldrich. .. Anti-Flag (M2, F3165), anti-GAPDH (SAB1405848), anti-YAP (WH0010413M1), and anti-TAZ (T4077) antibodies were from Sigma-Aldrich.

    Software:

    Article Title: RARβ2-dependent signaling represses neuronal differentiation in mouse ES cells
    Article Snippet: The blots were probed with anti-GAPDH (SAB2108266, 1:500, Sigma-Aldrich), anti-phospho-p38 MAPK (44-684G, 1:1000, Invitrogen/Thermo Fisher Scientific), and anti-phospho-PI3K (4292, 1:1000, Cell Signaling Technology). .. Quantitation of the bands was performed by densitometry tracing using ImageJ and/or the AlphaEase™ software.

    Article Title: Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells
    Article Snippet: Signals were visualized in a VersaDocTM imaging system (Bio-Rad) and analyzed using the ‘Quantity One’ software (Bio-Rad). .. The following primary antibodies were used: anti-Lig3 (1F3) mouse mAb (GeneTex); anti-GAPDH mouse mAb (Millipore).

    Negative Control:

    Article Title: microRNA-103 regulates the growth and invasion of endometrial cancer cells through the downregulation of tissue inhibitor of metalloproteinase 3
    Article Snippet: The anti-miR-103 oligonucleotide and control negative oligonucleotide (negative control) were purchased from Ambion (Carlsbad, CA, USA). .. The TIMP-3 antibody was purchased from Abcam (Cambridge, MA, USA), GAPDH antibody from Sigma (Steinheim, Germany) and goat anti-mouse IgG from Bio-Rad (Hercules, CA, USA).

    Selection:

    Article Title: PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65
    Article Snippet: The antibody against total PINK1 (S085D) was raised in sheep against a recombinant GST-PINK1 fragment (residues 175–250) and successively purified by positive and negative selection against recombinant fusion protein and GST, respectively. .. Anti-human PINK1 rabbit polyclonal (residues 175–250) antibody was obtained from Novus Biologicals; anti-GAPDH mouse monoclonal from Millipore; anti-Parkin mouse monoclonal (Santa Cruz).

    Incubation:

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma). .. Blots were incubated with the appropriate horseradish peroxidase-labeled secondary antibodies (Cell Signaling Technology, Danvers, MA).

    Article Title: Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma
    Article Snippet: .. After blocking with a 10% solution of defatted milk, the membranes were incubated overnight at 4°C with the following primary antibodies : anti-Kv1.3 (1:200, rabbit polyclonal, Alamone Labs APC-101); anti-GAPDH (1:1000, mouse monoclonal, Millipore MAB374). .. After washing, the membranes were developed using corresponding anti-mouse or anti-rabbit secondary antibodies (Calbiochem).

    Spectrophotometry:

    Article Title: Elevated nuclear Foxo1 suppresses excitability of skeletal muscle fibers
    Article Snippet: The supernatant was extracted and protein concentrations were measured using a Nanodrop-1000 spectrophotometer (Thermo Scientific). .. Blots were then processed and probed with antibodies against skeletal muscle voltage dependent sodium channel Nav1.4 (1:1,000; cat no. S9568; Sigma) and GAPDH (1:20,000; cat. no. G8795; Sigma).

    Concentration Assay:

    Article Title: Mahogunin regulates fusion between amphisomes/MVBs and lysosomes via ubiquitination of TSG101
    Article Snippet: Antibodies were from the following sources: CD63 (BD Pharmingen, #556019, San Jose, CA, USA), LAMP2 (H4B4, Hybridoma Technology, Iowa City, IA, USA), Beclin1 (Novus Biologicals, #NB500-249, Littleton, CO, USA), LC3 (Novus Biologicals, #NB100-2220), p62/ SQSTM1 (Thermo Scientific, #PA5-20839, Rockford, IL, USA), β- catenin (Cell Signaling Technology, #9562, Danvers, MA, USA), Monoclonal anti GAPDH clone GAPDH-71.1 (Sigma-Aldrich, #G8795, St. Louis, MO, USA), EGFR (A10) (Santa Cruz Biotechnology, Inc., #sc-373746, Dallas, TX, USA), Rab7 (Abcam, #ab50533, Cambridge, UK), β -tubulin (Abcam, #ab7792), TSG101(Abcam, #ab83), Ub (Sigma-Aldrich, #U0508, Anti-Cathepsin D (CTD-19, Abcam, #ab6313)). .. MG132 (Sigma-Aldrich, #C2211) was used at 10 μ M concentration for 6 h. Bafilomycin A1 (Sigma-Aldrich, #B1793) was used at 300 nM concentration for 10 h for HeLa, 60 nM concentration for 7 h for SHSY5Y and 50 nM concentration for 4 h for Melan cells.

    Lysis:

    Article Title: Tyrosine kinase FYN negatively regulates NOX4 in cardiac remodeling
    Article Snippet: Heart homogenates and CM lysates were prepared in RIPA lysis buffer containing 50 mM Tris (pH 7.5), 150 mM NaCl, 1% IGEPAL CA-630 (Sigma Aldrich), 0.1% SDS, 0.5% deoxycholic acid, 10 mM Na4 P2 O7 , 5 mM EDTA, 0.1 mM Na3 VO4 , 1 mM NaF, 0.5 mM 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF), 0.5 μg/ml aprotinin, and 0.5 μg/ml leupeptin. .. For immunoblot analyses, we used monoclonal antibodies raised against NOX4, as previously described , GAPDH (catalog G8795), tubulin (catalog T6074) (both Sigma-Aldrich), COX IV (catalog ab14744; Abcam), and polyclonal antibodies raised against FYN (catalog sc-28791), Src (catalog sc-5266), YES (catalog sc-46674), and p22phox (catalog sc-20781) (all Santa Cruz Biotechnology), phospho-Src (Tyr416) (catalog 2101), His-tag (catalog 2366), HA-tag (catalog 3724), and S-tag (catalog 8476) (all Cell Signaling Technology).

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  • 90
    Millipore mouse anti bax monoclonal
    Effects of Gli-1 downregulation on the expression of apoptosis-related proteins in LN229 cells. (A,B) Cultured LN229 cells were transfected with non-specific siRNA (siNC) and two Gli-1-specific siRNAs (siGli-1-1 and siGli-1-2) for 24 h. Then, the expression of <t>pro-caspase-8/9/3</t> (A) and <t>Bcl-2/Bax</t> (B) was determined by western blot (WB) analysis. Shh protein (5 μg/mL) was simultaneously added to the non-transfected LN229 cells as a positive control. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) ,−9 (D) , and−3 (E) , Bcl-2 (F) , and Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) ; each statistical value represents the mean ± SD of three independent experiments ( n = 5). * P
    Mouse Anti Bax Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti bax monoclonal/product/Millipore
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    92
    Millipore mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
    Sumoylation of FOXP1. ( A ) Representative immunoblotting of Foxp1 in the mouse neocortex during development. ( Right panel) Quantification of SUMO–Foxp1 immunoblotting. Immunoblots were first normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH)</t> at each time point and then subsequently normalized to nonsumoylated Foxp1 levels at embryonic day 15.5 (E15.5). Data are represented as means (±SEM). n = 3 per condition. ( B ) Endogenous coimmunoprecipitation of Foxp1 and SUMO-1 in the mouse neocortex at P0. ( C ) Schematic of FOXP1 protein showing the location of K636. (PolyQ) Polyglutamine motif; (ZF) zinc finger; (LZ) leucine zipper. ( D ) K636 is conserved across species. ( E ) Immunoblotting for Flag-tagged FOXP1 in 293T cells. Lysates were treated with 1 mM H 2 O 2 for 1 h ( left panel) or 100 µM ginkgolic acid for 6 h ( right panel) in the presence or absence of NEM. (FOXP1 WT) Flag-tagged wild-type FOXP1. The asterisk indicates a nonspecific band of the SUMO-1 antibody. ( F ) Immunoblot of immunoprecipitated wild-type Flag-tagged FOXP1 or Flag-tagged FOXP1 KR.
    Mouse Monoclonal Anti Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody - by Bioz Stars, 2020-04
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    90
    Millipore rabbit anti bax
    Effects of coffee oil-algae oil nanoemulsions on protein expressions of <t>cyclin</t> B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and <t>Bax,</t> Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p
    Rabbit Anti Bax, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti bax/product/Millipore
    Average 90 stars, based on 9 article reviews
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    Effects of Gli-1 downregulation on the expression of apoptosis-related proteins in LN229 cells. (A,B) Cultured LN229 cells were transfected with non-specific siRNA (siNC) and two Gli-1-specific siRNAs (siGli-1-1 and siGli-1-2) for 24 h. Then, the expression of pro-caspase-8/9/3 (A) and Bcl-2/Bax (B) was determined by western blot (WB) analysis. Shh protein (5 μg/mL) was simultaneously added to the non-transfected LN229 cells as a positive control. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) ,−9 (D) , and−3 (E) , Bcl-2 (F) , and Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) ; each statistical value represents the mean ± SD of three independent experiments ( n = 5). * P

    Journal: Frontiers in Neuroscience

    Article Title: Activity of Metabotropic Glutamate Receptor 4 Suppresses Proliferation and Promotes Apoptosis With Inhibition of Gli-1 in Human Glioblastoma Cells

    doi: 10.3389/fnins.2018.00320

    Figure Lengend Snippet: Effects of Gli-1 downregulation on the expression of apoptosis-related proteins in LN229 cells. (A,B) Cultured LN229 cells were transfected with non-specific siRNA (siNC) and two Gli-1-specific siRNAs (siGli-1-1 and siGli-1-2) for 24 h. Then, the expression of pro-caspase-8/9/3 (A) and Bcl-2/Bax (B) was determined by western blot (WB) analysis. Shh protein (5 μg/mL) was simultaneously added to the non-transfected LN229 cells as a positive control. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) ,−9 (D) , and−3 (E) , Bcl-2 (F) , and Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) ; each statistical value represents the mean ± SD of three independent experiments ( n = 5). * P

    Article Snippet: The primary antibodies and dilutions used in the experiments were as follows: rabbit anti-mGluR4 (1:1,000, Abcam); rabbit anti-Gli-1 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 3 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 8 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 9 polyclonal (1:1,000, Cell Signaling Technology); mouse anti-Bcl-2 monoclonal (1:1,000, Millipore); mouse anti-Bax monoclonal (1:1,000, Millipore); mouse anti-cyclin D1 monoclonal (1:1,000, Cell Signaling Technology); mouse anti-β-actin monoclonal (1:10,000, Sigma-Aldrich).

    Techniques: Expressing, Cell Culture, Transfection, Western Blot, Positive Control

    Effects of mGluR4 activation on the expression of apoptosis-related proteins in LN229 cells. (A,B) Cultured LN229 cells were transfected with non-specific siRNA (siNC) and two mGluR4-specific siRNAs (simGluR4-1 and simGluR4-2) for 24 h, followed by treatment with the vehicle (Ctrl) or 30 μM of VU0155041 for 24 h. Then, the differential expression of pro-caspase-8/9/3 (A) and Bcl-2/Bax (B) was determined by western blot (WB) analysis. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) , 9 (D) , 3 (E) , Bcl-2 (F) , Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) , each statistical value represents the mean ± SD of at least three independent experiments. * P

    Journal: Frontiers in Neuroscience

    Article Title: Activity of Metabotropic Glutamate Receptor 4 Suppresses Proliferation and Promotes Apoptosis With Inhibition of Gli-1 in Human Glioblastoma Cells

    doi: 10.3389/fnins.2018.00320

    Figure Lengend Snippet: Effects of mGluR4 activation on the expression of apoptosis-related proteins in LN229 cells. (A,B) Cultured LN229 cells were transfected with non-specific siRNA (siNC) and two mGluR4-specific siRNAs (simGluR4-1 and simGluR4-2) for 24 h, followed by treatment with the vehicle (Ctrl) or 30 μM of VU0155041 for 24 h. Then, the differential expression of pro-caspase-8/9/3 (A) and Bcl-2/Bax (B) was determined by western blot (WB) analysis. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) , 9 (D) , 3 (E) , Bcl-2 (F) , Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) , each statistical value represents the mean ± SD of at least three independent experiments. * P

    Article Snippet: The primary antibodies and dilutions used in the experiments were as follows: rabbit anti-mGluR4 (1:1,000, Abcam); rabbit anti-Gli-1 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 3 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 8 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 9 polyclonal (1:1,000, Cell Signaling Technology); mouse anti-Bcl-2 monoclonal (1:1,000, Millipore); mouse anti-Bax monoclonal (1:1,000, Millipore); mouse anti-cyclin D1 monoclonal (1:1,000, Cell Signaling Technology); mouse anti-β-actin monoclonal (1:10,000, Sigma-Aldrich).

    Techniques: Activation Assay, Expressing, Cell Culture, Transfection, Western Blot

    Gli-1 downregulation is involved in mGluR4-mediated dynamic expression of apoptosis-related proteins in LN229 cells. (A,B) LN229 cells were treated with the vehicle (Ctrl), 5 μg/mL shh, or 30 μM VU0155041 plus 5 μg/mL shh (VU + shh) for 24 h. Then, the expression of pro-caspase-8/9/3 (A) and Bcl-2/Bax (B) was determined by western blot (WB) analysis. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) ,−9 (D) , and−3 (E) , Bcl-2 (F) , and Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) ; each statistical value represents the mean ± SD of three independent experiments. * P

    Journal: Frontiers in Neuroscience

    Article Title: Activity of Metabotropic Glutamate Receptor 4 Suppresses Proliferation and Promotes Apoptosis With Inhibition of Gli-1 in Human Glioblastoma Cells

    doi: 10.3389/fnins.2018.00320

    Figure Lengend Snippet: Gli-1 downregulation is involved in mGluR4-mediated dynamic expression of apoptosis-related proteins in LN229 cells. (A,B) LN229 cells were treated with the vehicle (Ctrl), 5 μg/mL shh, or 30 μM VU0155041 plus 5 μg/mL shh (VU + shh) for 24 h. Then, the expression of pro-caspase-8/9/3 (A) and Bcl-2/Bax (B) was determined by western blot (WB) analysis. (C–H) WB bands were quantified to generate the ratios of pro-caspase-8 (C) ,−9 (D) , and−3 (E) , Bcl-2 (F) , and Bax (G) to β-actin and the ratio of Bcl-2 to Bax (H) ; each statistical value represents the mean ± SD of three independent experiments. * P

    Article Snippet: The primary antibodies and dilutions used in the experiments were as follows: rabbit anti-mGluR4 (1:1,000, Abcam); rabbit anti-Gli-1 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 3 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 8 polyclonal (1:1,000, Cell Signaling Technology); rabbit anti-caspase 9 polyclonal (1:1,000, Cell Signaling Technology); mouse anti-Bcl-2 monoclonal (1:1,000, Millipore); mouse anti-Bax monoclonal (1:1,000, Millipore); mouse anti-cyclin D1 monoclonal (1:1,000, Cell Signaling Technology); mouse anti-β-actin monoclonal (1:10,000, Sigma-Aldrich).

    Techniques: Expressing, Western Blot

    Sumoylation of FOXP1. ( A ) Representative immunoblotting of Foxp1 in the mouse neocortex during development. ( Right panel) Quantification of SUMO–Foxp1 immunoblotting. Immunoblots were first normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) at each time point and then subsequently normalized to nonsumoylated Foxp1 levels at embryonic day 15.5 (E15.5). Data are represented as means (±SEM). n = 3 per condition. ( B ) Endogenous coimmunoprecipitation of Foxp1 and SUMO-1 in the mouse neocortex at P0. ( C ) Schematic of FOXP1 protein showing the location of K636. (PolyQ) Polyglutamine motif; (ZF) zinc finger; (LZ) leucine zipper. ( D ) K636 is conserved across species. ( E ) Immunoblotting for Flag-tagged FOXP1 in 293T cells. Lysates were treated with 1 mM H 2 O 2 for 1 h ( left panel) or 100 µM ginkgolic acid for 6 h ( right panel) in the presence or absence of NEM. (FOXP1 WT) Flag-tagged wild-type FOXP1. The asterisk indicates a nonspecific band of the SUMO-1 antibody. ( F ) Immunoblot of immunoprecipitated wild-type Flag-tagged FOXP1 or Flag-tagged FOXP1 KR.

    Journal: Genes & Development

    Article Title: Foxp1 regulation of neonatal vocalizations via cortical development

    doi: 10.1101/gad.305037.117

    Figure Lengend Snippet: Sumoylation of FOXP1. ( A ) Representative immunoblotting of Foxp1 in the mouse neocortex during development. ( Right panel) Quantification of SUMO–Foxp1 immunoblotting. Immunoblots were first normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) at each time point and then subsequently normalized to nonsumoylated Foxp1 levels at embryonic day 15.5 (E15.5). Data are represented as means (±SEM). n = 3 per condition. ( B ) Endogenous coimmunoprecipitation of Foxp1 and SUMO-1 in the mouse neocortex at P0. ( C ) Schematic of FOXP1 protein showing the location of K636. (PolyQ) Polyglutamine motif; (ZF) zinc finger; (LZ) leucine zipper. ( D ) K636 is conserved across species. ( E ) Immunoblotting for Flag-tagged FOXP1 in 293T cells. Lysates were treated with 1 mM H 2 O 2 for 1 h ( left panel) or 100 µM ginkgolic acid for 6 h ( right panel) in the presence or absence of NEM. (FOXP1 WT) Flag-tagged wild-type FOXP1. The asterisk indicates a nonspecific band of the SUMO-1 antibody. ( F ) Immunoblot of immunoprecipitated wild-type Flag-tagged FOXP1 or Flag-tagged FOXP1 KR.

    Article Snippet: The following antibodies were used: mouse monoclonal anti-SUMO-1 (D-11) antibody (Santa Cruz Biotechnology, sc-5308), rabbit polyclonal anti-FOXP1 antibody , mouse monoclonal anti-FOXP1 (JC12) antibody (Abcam, ab32010), goat polyclonal anti-FOXP2 (N-16) antibody (Santa Cruz Biotechnology, sc-21068), mouse monoclonal anti-Flag M2 antibody (Sigma-Aldrich, F1804), mouse monoclonal anti-V5 antibody (Invitrogen, R960-25), goat polyclonal anti-GFP antibody (Rockland Immunochemicals, 600-101-215), chick polyclonal anti-GFP antibody (Aves Laboratories, GFP-1010), rabbit monoclonal anti-SUMO-2/3 (18H8) antibody (Cell Signaling Technology, 4971), rabbit polyclonal anti-PIAS2 antibody (Abcam, ab155556), rabbit polyclonal anti-PIAS3 (H-169) antibody (Santa Cruz Biotechnology, sc-14017), rabbit polyclonal anti-MAP2 antibody (Chemicon, AB5622), mouse monoclonal anti-CtBP (E-12) antibody (Santa Cruz Biotechnology, sc-17759), rabbit polyclonal anti-CDP (CUX1: M-222) antibody (Santa Cruz Biotechnology, sc-13024), rat anti-CTIP2 (Abcam, ab18465), rabbit polyclonal anti-HDAC1 antibody (Abcam, ab19845), mouse monoclonal anti-HDAC1 (10E2) antibody (Cell Signaling Technology, 5256), mouse monoclonal anti-HDAC2 (3F3) antibody (Cell Signaling Technology, 5113), rabbit monoclonal anti-MTA1 (D40D1) XP antibody (Cell Signaling Technology, 5647), rabbit polyclonal anti-MTA2 (H-170) antibody (Santa Cruz Biotechnology, sc-28731), rabbit polyclonal anti-p66β (GATAD2B) antibody (Novus Biologicals, NBP1-87358), mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Millipore, MAB374), mouse (G3A1) mAb IgG1 isotype control (Cell Signaling Technology, 5415), normal rabbit IgG (Cell Signaling Technology, 2729), and normal goat IgG (Santa Cruz Biotechnology, sc-2028).

    Techniques: Western Blot, Immunoprecipitation

    Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

    doi: 10.2147/IJN.S144705

    Figure Lengend Snippet: Effects of coffee oil-algae oil nanoemulsions on protein expressions of cyclin B, CDK2, cyclin A, and CDK1 ( A ), p53 and p21 ( B ), and Bax, Bcl-2, cytochrome C ( C ). Notes: Control cells are incubated with medium only. Results are presented as mean ± standard deviation of triplicate determinations. Data with different letters (A–C) are significantly different at p

    Article Snippet: The primary antibodies include mouse monoclonal anti-α-tubulin antibody (Sigma–Aldrich Co.), mouse anti-cyclin A and rabbit anti-Bax (EMD Millipore), mouse anti-CDK2, mouse anti-cytochrome C, mouse anti-p21, mouse anti-cyclin B, and mouse anti-Bcl-2 (BD Biosciences), as well as anti-cdc2 (CDK1) and mouse anti-p53 (Novus Biologicals Co., Littleton, CO, USA).

    Techniques: Incubation, Standard Deviation