glyceraldehyde 3 phosphate dehydrogenase gapdh  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Millipore glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effect of azole antifungal compounds on protein expression. MCF-7 and MDA-MB-231 cells were treated with 50 μM imidazole [clotrimazole (CTZ) or ketoconazole (KCZ)] or triazole [fluconazole (FCZ) or itraconazole (ICZ)] compounds for 24 h. The protein expression related to apoptosis (A) and cell cycle (B) were examined in MCF-7 and MDA-MB-231 cells after treatment of each compound for 24 h. The protein expression was also examined in the xenograft model of MDA-MB-231 cells after treatment of each compound for 8 weeks (C). Glyceraldehyde 3-phosphate dehydrogenase <t>(GAPDH)</t> was used as the loading control. This experiment was performed three times. Band density was estimated using ImageJ 1.45s software (NIH).
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Millipore
    Average 95 stars, based on 147 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-01
    95/100 stars

    Images

    1) Product Images from "Imidazole Antifungal Drugs Inhibit the Cell Proliferation and Invasion of Human Breast Cancer Cells"

    Article Title: Imidazole Antifungal Drugs Inhibit the Cell Proliferation and Invasion of Human Breast Cancer Cells

    Journal: Biomolecules & Therapeutics

    doi: 10.4062/biomolther.2018.042

    Effect of azole antifungal compounds on protein expression. MCF-7 and MDA-MB-231 cells were treated with 50 μM imidazole [clotrimazole (CTZ) or ketoconazole (KCZ)] or triazole [fluconazole (FCZ) or itraconazole (ICZ)] compounds for 24 h. The protein expression related to apoptosis (A) and cell cycle (B) were examined in MCF-7 and MDA-MB-231 cells after treatment of each compound for 24 h. The protein expression was also examined in the xenograft model of MDA-MB-231 cells after treatment of each compound for 8 weeks (C). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the loading control. This experiment was performed three times. Band density was estimated using ImageJ 1.45s software (NIH).
    Figure Legend Snippet: Effect of azole antifungal compounds on protein expression. MCF-7 and MDA-MB-231 cells were treated with 50 μM imidazole [clotrimazole (CTZ) or ketoconazole (KCZ)] or triazole [fluconazole (FCZ) or itraconazole (ICZ)] compounds for 24 h. The protein expression related to apoptosis (A) and cell cycle (B) were examined in MCF-7 and MDA-MB-231 cells after treatment of each compound for 24 h. The protein expression was also examined in the xenograft model of MDA-MB-231 cells after treatment of each compound for 8 weeks (C). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the loading control. This experiment was performed three times. Band density was estimated using ImageJ 1.45s software (NIH).

    Techniques Used: Expressing, Multiple Displacement Amplification, Software

    2) Product Images from "Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen"

    Article Title: Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0029463

    Effects of ethanol and androgenization on profibrotic gene expression. Quantitative Real-Time PCR analysis of Interleukin-6 (IL-6) (black bar) and collagen Type 3 (COL3α1) (striped bar) gene expression in control (Ctrl), androgenized (Andro) and Andro + estrogen (Andro + E2) animals maintained on control Lieber-DeCarli (C-LDC) or ethanol-LDC (E-LDC) diet. Gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are presented as mean values ± SEM. *P
    Figure Legend Snippet: Effects of ethanol and androgenization on profibrotic gene expression. Quantitative Real-Time PCR analysis of Interleukin-6 (IL-6) (black bar) and collagen Type 3 (COL3α1) (striped bar) gene expression in control (Ctrl), androgenized (Andro) and Andro + estrogen (Andro + E2) animals maintained on control Lieber-DeCarli (C-LDC) or ethanol-LDC (E-LDC) diet. Gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data are presented as mean values ± SEM. *P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    3) Product Images from "Effects of Progesterone Treatment on Expression of Genes Involved in Uterine Quiescence"

    Article Title: Effects of Progesterone Treatment on Expression of Genes Involved in Uterine Quiescence

    Journal: Reproductive Sciences

    doi: 10.1177/1933719111398150

    Immuoblot of Maxi-K channel α-subunit (KCNMA1) in triplicate samples of cells treated either with estrogen (E) or estrogen plus progesterone (E + P). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) staining illustrates the uniformity of gel loading
    Figure Legend Snippet: Immuoblot of Maxi-K channel α-subunit (KCNMA1) in triplicate samples of cells treated either with estrogen (E) or estrogen plus progesterone (E + P). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) staining illustrates the uniformity of gel loading

    Techniques Used: Staining

    Related Articles

    Diagnostic Assay:

    Article Title: Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen
    Article Snippet: For Western blot analyses, antibodies against cytochrome P450 2E1 (CYP2E1), cytochrome P450 1A2 (CYP1A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA). .. For Western blot analyses, antibodies against cytochrome P450 2E1 (CYP2E1), cytochrome P450 1A2 (CYP1A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA).

    Nucleic Acid Electrophoresis:

    Article Title: Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats
    Article Snippet: Protein extracts (50 μg) were heated at 100°C for 5 min and size fractionated by Bis-Tris sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Invitrogen, Carlsbad, CA, USA). .. Protein samples were denatured, separated by SDS-PAGE, transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA), blocked with 5% fat-free milk in Tris-buffered saline (2 h, room temperature), and incubated overnight at 4°C with primary antibody against BMP7 (diluted 1:500) and mouse monoclonal antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (diluted 1:15000; Sigma, St Louis, MO, USA).

    Article Title: Antiviral activities of Indonesian medicinal plants in the East Java region against hepatitis C virus
    Article Snippet: Immunoblotting Cells were lysed with SDS sample buffer, and equal amounts of protein were subjected to SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA), which was then incubated with the respective primary antibody. .. The primary antibodies used were mouse monoclonal antibodies against HCV NS3 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Millipore).

    Article Title: Calcitriol downregulates fibroblast growth factor receptor 1 through histone deacetylase activation in HL-1 atrial myocytes
    Article Snippet: Proteins were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) under reducing conditions and electrophoretically transferred onto equilibrated polyvinylidene difluoride membranes (Amersham Biosciences, Buckinghamshire, UK), as described in a previous study [ ]. .. Targeted bands were normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma) or lamin B to confirm equal protein loading.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effects of Progesterone Treatment on Expression of Genes Involved in Uterine Quiescence
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for endothelin 1 and cyclic adenosine monophosphate (cAMP) were purchased from BD Biosciences (San Jose, California) and used according to the manufacturer’s directions. .. Monoclonal antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from Millipore Corp (Billerica, Massachusetts).

    Incubation:

    Article Title: Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats
    Article Snippet: .. Protein samples were denatured, separated by SDS-PAGE, transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA), blocked with 5% fat-free milk in Tris-buffered saline (2 h, room temperature), and incubated overnight at 4°C with primary antibody against BMP7 (diluted 1:500) and mouse monoclonal antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (diluted 1:15000; Sigma, St Louis, MO, USA). ..

    Article Title: Regular Exercise Prevents Sleep Deprivation Associated Impairment of Long-Term Memory and Synaptic Plasticity in The CA1 Area of the Hippocampus
    Article Snippet: To measure the levels of a protein, we incubated with polyclonal or monoclonal primary antibodies as outlined below, and the immunoreactive bands were detected by a horseradish peroxidase-conjugated secondary antibody. .. Mouse and rabbit polyclonal antibody for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Millipore, Temacula, CA; 1:2000 dilutions) was used to detect GAPDH (loading control) protein level, and the band intensities of all signaling molecules were expressed as a ratio to the GAPDH intensity.

    Article Title: Antiviral activities of Indonesian medicinal plants in the East Java region against hepatitis C virus
    Article Snippet: Immunoblotting Cells were lysed with SDS sample buffer, and equal amounts of protein were subjected to SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (Millipore, Bedford, MA), which was then incubated with the respective primary antibody. .. The primary antibodies used were mouse monoclonal antibodies against HCV NS3 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Millipore).

    Expressing:

    Article Title: Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
    Article Snippet: .. For the Western blot analysis, cell death expression in whole-brain tissue was defined as the total mean protein expression of caspase-3, normalized to the commonly used glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich Corp, St Louis, MO, USA)., .. Cell counting Photomicrographs were taken using an Eclipse 90i upright microscope (Nikon Corporation, Tokyo, Japan) and a Nikon DS-Fi1 digital camera (Nikon) (1,280×960 pixels).

    BIA-KA:

    Article Title: Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen
    Article Snippet: A BCA kit to measure protein concentrations was purchased from Pierce Biotechnology, Inc (Santa Cruz, CA) and 540 nm absorbance was measured on a BioTek Synergy HT Multi-Detection Microplate Reader (BioTek Instruments, Winooski, VT) using Gen5 Analysis Software. .. For Western blot analyses, antibodies against cytochrome P450 2E1 (CYP2E1), cytochrome P450 1A2 (CYP1A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA).

    Modification:

    Article Title: Combination Therapy of Chloroquine and C2-Ceramide Enhances Cytotoxicity in Lung Cancer H460 and H1299 Cells
    Article Snippet: Reagents Dulbecco’s modified Eagle’s medium (DMEM) was purchased from HyClone (Logan, UT, USA). .. Antibodies against p-Src, Src, p-SIRT1, SIRT1 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Calbiochem (La Jolla, CA, USA).

    Western Blot:

    Article Title: Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen
    Article Snippet: .. For Western blot analyses, antibodies against cytochrome P450 2E1 (CYP2E1), cytochrome P450 1A2 (CYP1A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA). .. Antibody against 4-Hydroxynonenal (4-HNE) was purchased from Alpha Diagnostic Intl (San Antonio, TX).

    Article Title: Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats
    Article Snippet: Paragraph title: Protein preparation and western blot ... Protein samples were denatured, separated by SDS-PAGE, transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA), blocked with 5% fat-free milk in Tris-buffered saline (2 h, room temperature), and incubated overnight at 4°C with primary antibody against BMP7 (diluted 1:500) and mouse monoclonal antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (diluted 1:15000; Sigma, St Louis, MO, USA).

    Article Title: Regulation of Mitochondrial Biogenesis by Lipoprotein Lipase in Muscle of Insulin-Resistant Offspring of Parents With Type 2 Diabetes
    Article Snippet: Paragraph title: Western blot assay. ... Equal protein loading was confirmed by reblotting of the membranes with either a goat polyclonal antibody to pan-actin (1:1,000; Santa Cruz Biotechnology Inc.) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000; Chemicon).

    Article Title: Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
    Article Snippet: .. For the Western blot analysis, cell death expression in whole-brain tissue was defined as the total mean protein expression of caspase-3, normalized to the commonly used glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich Corp, St Louis, MO, USA)., .. Cell counting Photomicrographs were taken using an Eclipse 90i upright microscope (Nikon Corporation, Tokyo, Japan) and a Nikon DS-Fi1 digital camera (Nikon) (1,280×960 pixels).

    Article Title: mTOR Inhibition Specifically Sensitizes Colorectal Cancers with KRAS or BRAF Mutations to BCL-2/BCL-XL Inhibition by Suppressing MCL-1
    Article Snippet: The following antibodies were used for Western blot analyses: phospho-AKT (473) (cat #9271), phospho-RPS6 (240/244) (cat #2211), phospho-4EBP1 (cat #2855), phospho-ERK (cat #9101), total RPS6 (cat #2217), phospho-4EBP1 (cat #2855), BIM (cat #2819), BAK (cat #3814) and BCL-XL (cat #2762) were from Cell Signaling (Beverly, MA). .. Antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was purchased from Chemicon.

    Article Title: Calcitriol downregulates fibroblast growth factor receptor 1 through histone deacetylase activation in HL-1 atrial myocytes
    Article Snippet: Paragraph title: Western blot analysis ... Targeted bands were normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma) or lamin B to confirm equal protein loading.

    Real-time Polymerase Chain Reaction:

    Article Title: DUSP10 Negatively Regulates the Inflammatory Response to Rhinovirus through Interleukin-1β Signaling
    Article Snippet: PCR was carried out using a GoTaq hot start polymerase kit (Promega) according to the manufacturer’s instructions, using primers specific to DUSPs 1, 4, and 10 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich). .. Quantitative PCR was carried out using primers and probes from Sigma-Aldrich for RV (SY150600935-024, SY150600935-025, and HA07878670-002) and IFN-β (SY150506722-061, SY150504450-060, and HA07784503-002) and primer-probe sets from Applied Biosystems for DUSP1 (Hs00610256_g1), DUSP4 (Hs01027785_m1), DUSP10 (Hs00200527_m1), CXCL8 (Hs00174103_m1), and GAPDH (Hs02758991_g1).

    Article Title: Prolonged lymphocytosis during ibrutinib therapy is associated with distinct molecular characteristics and does not indicate a suboptimal response to therapy
    Article Snippet: Phospho-Erk(Thr202/Tyr204) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA). .. Real-time reverse transcription–polymerase chain reaction (RT-PCR) was performed using Taqman primers from Applied Biosystems (Foster City, CA), and was performed on an Applied Biosystems Viia7 Real Time PCR System.

    Generated:

    Article Title: DUSP10 Negatively Regulates the Inflammatory Response to Rhinovirus through Interleukin-1β Signaling
    Article Snippet: RNA was extracted using Tri reagent (Sigma-Aldrich) according to the manufacturer’s instructions, and contaminating DNA was removed using a DNase treatment kit (Ambion). cDNA was generated from 1 μg RNA by use of a high-capacity cDNA reverse transcriptase kit (Applied Biosystems). .. PCR was carried out using a GoTaq hot start polymerase kit (Promega) according to the manufacturer’s instructions, using primers specific to DUSPs 1, 4, and 10 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich).

    Imaging:

    Article Title: Regular Exercise Prevents Sleep Deprivation Associated Impairment of Long-Term Memory and Synaptic Plasticity in The CA1 Area of the Hippocampus
    Article Snippet: Subsequently, the blots were developed using chemiluminescence reagent, detected in an Alpha Innotech imaging system, and quantified by densitometry. .. Mouse and rabbit polyclonal antibody for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Millipore, Temacula, CA; 1:2000 dilutions) was used to detect GAPDH (loading control) protein level, and the band intensities of all signaling molecules were expressed as a ratio to the GAPDH intensity.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Prolonged lymphocytosis during ibrutinib therapy is associated with distinct molecular characteristics and does not indicate a suboptimal response to therapy
    Article Snippet: Paragraph title: Immunoblotting and real-time reverse-transcription–polymerase chain reaction ... Phospho-Erk(Thr202/Tyr204) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA).

    Sonication:

    Article Title: Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats
    Article Snippet: Protein preparation was performed as described previously [ ]: cloaca/hindgut were pooled and sonicated in distilled deionized water containing protease inhibitors. .. Protein samples were denatured, separated by SDS-PAGE, transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA), blocked with 5% fat-free milk in Tris-buffered saline (2 h, room temperature), and incubated overnight at 4°C with primary antibody against BMP7 (diluted 1:500) and mouse monoclonal antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (diluted 1:15000; Sigma, St Louis, MO, USA).

    MTT Assay:

    Article Title: Imidazole Antifungal Drugs Inhibit the Cell Proliferation and Invasion of Human Breast Cancer Cells
    Article Snippet: Materials CTZ, KCZ, FCZ, ICZ, dimethylsulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT), mitomycin C, Matrigel, and collagen were obtained from Sigma-Aldrich (St. Louis, MO, USA). .. Cyclin D1, cyclin E1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Sigma-Aldrich.

    Polymerase Chain Reaction:

    Article Title: DUSP10 Negatively Regulates the Inflammatory Response to Rhinovirus through Interleukin-1β Signaling
    Article Snippet: .. PCR was carried out using a GoTaq hot start polymerase kit (Promega) according to the manufacturer’s instructions, using primers specific to DUSPs 1, 4, and 10 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: Calcitriol downregulates fibroblast growth factor receptor 1 through histone deacetylase activation in HL-1 atrial myocytes
    Article Snippet: Proteins were subjected to SDS-polyacrylamide gel electrophoresis (PAGE) under reducing conditions and electrophoretically transferred onto equilibrated polyvinylidene difluoride membranes (Amersham Biosciences, Buckinghamshire, UK), as described in a previous study [ ]. .. Targeted bands were normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma) or lamin B to confirm equal protein loading.

    SDS Page:

    Article Title: Spatiotemporal expression of BMP7 in the development of anorectal malformations in fetal rats
    Article Snippet: .. Protein samples were denatured, separated by SDS-PAGE, transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA), blocked with 5% fat-free milk in Tris-buffered saline (2 h, room temperature), and incubated overnight at 4°C with primary antibody against BMP7 (diluted 1:500) and mouse monoclonal antibody to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (diluted 1:15000; Sigma, St Louis, MO, USA). ..

    Software:

    Article Title: Neonatal Androgenization Exacerbates Alcohol-Induced Liver Injury in Adult Rats, an Effect Abrogated by Estrogen
    Article Snippet: A BCA kit to measure protein concentrations was purchased from Pierce Biotechnology, Inc (Santa Cruz, CA) and 540 nm absorbance was measured on a BioTek Synergy HT Multi-Detection Microplate Reader (BioTek Instruments, Winooski, VT) using Gen5 Analysis Software. .. For Western blot analyses, antibodies against cytochrome P450 2E1 (CYP2E1), cytochrome P450 1A2 (CYP1A2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Millipore (Billerica, MA).

    Article Title: Calcitriol downregulates fibroblast growth factor receptor 1 through histone deacetylase activation in HL-1 atrial myocytes
    Article Snippet: Bound antibodies were detected using an enhanced chemiluminescence detection system (Millipore) and analyzed using AlphaEaseFC software (Alpha Innotech, San Leandro, CA). .. Targeted bands were normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma) or lamin B to confirm equal protein loading.

    ROS Assay:

    Article Title: Grape seed-derived procyanidins alleviate gout pain via NLRP3 inflammasome suppression
    Article Snippet: Antibody for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was from Sigma-Aldrich (St. Louis, MO, USA). .. ROS Assay Kit was from KeyGEN (Nanjing, China).

    SDS-Gel:

    Article Title: Regulation of Mitochondrial Biogenesis by Lipoprotein Lipase in Muscle of Insulin-Resistant Offspring of Parents With Type 2 Diabetes
    Article Snippet: Samples were denatured with Laemmli sample buffer for 5 min at 95°C and 40-μg samples were separated using 7.5–15% SDS gel (Bio-Rad Laboratories Inc.) and electrotransfered onto polyvinylidene fluoride membranes (Amersham Pharmacia Biotech Inc.). .. Equal protein loading was confirmed by reblotting of the membranes with either a goat polyclonal antibody to pan-actin (1:1,000; Santa Cruz Biotechnology Inc.) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000; Chemicon).

    Radio Immunoprecipitation:

    Article Title: Terminalia chebula extract prevents scopolamine-induced amnesia via cholinergic modulation and anti-oxidative effects in mice
    Article Snippet: Anti-ChAT and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from EMD Millipore (Billerica, MA, USA). .. Radioimmunoprecipitation assay (RIPA) buffer was obtained from Thermo Scientific (Waltham, MA, USA).

    Homogenization:

    Article Title: Regular Exercise Prevents Sleep Deprivation Associated Impairment of Long-Term Memory and Synaptic Plasticity in The CA1 Area of the Hippocampus
    Article Snippet: Paragraph title: Homogenization and Immunoblotting ... Mouse and rabbit polyclonal antibody for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Millipore, Temacula, CA; 1:2000 dilutions) was used to detect GAPDH (loading control) protein level, and the band intensities of all signaling molecules were expressed as a ratio to the GAPDH intensity.

    Marker:

    Article Title: Fish oil supplementation associated with decreased cellular degeneration and increased cellular proliferation 6 weeks after middle cerebral artery occlusion in the rat
    Article Snippet: Cellular markers We examined the relationship between n-3-LC-PUFA supplementation and the number of caspase-3 (Polyclonal Cleaved Caspase-3 [Asp175] Antibody; Genesearch, Arundel, Australia) and Ki-67-like immunoreactivity (Ki-67-ir)-labeled cells (Anti-Ki-67 antibody [SP6] – Monoclonal Proliferation Marker; Sapphire Bioscience, San Diego, CA, USA), as a general marker of cellular proliferation. .. For the Western blot analysis, cell death expression in whole-brain tissue was defined as the total mean protein expression of caspase-3, normalized to the commonly used glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Sigma-Aldrich Corp, St Louis, MO, USA).,

    Lysis:

    Article Title: Regulation of Mitochondrial Biogenesis by Lipoprotein Lipase in Muscle of Insulin-Resistant Offspring of Parents With Type 2 Diabetes
    Article Snippet: In brief, samples were homogenized in lysis buffer containing 50 mmol/L HEPES (pH 7.4), 150 mmol/L NaCl, 1 mmol/L EDTA, 1% Triton X-100, 2 mmol/L sodium vanadate, 100 mmol/L NaF, 20 mmol/L sodium pyrophosphate, 20 μg/mL aprotinin, and 1 mmol/L phenylmethylsulfonyl fluoride. .. Equal protein loading was confirmed by reblotting of the membranes with either a goat polyclonal antibody to pan-actin (1:1,000; Santa Cruz Biotechnology Inc.) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:5,000; Chemicon).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 82
    Millipore gylceraldehyde 3 phosphate dehydrogenase
    Gylceraldehyde 3 Phosphate Dehydrogenase, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gylceraldehyde 3 phosphate dehydrogenase/product/Millipore
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gylceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    90
    Millipore anti bax
    Anti Bax, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax/product/Millipore
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti bax - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Millipore anti bax antibody
    Anti Bax Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax antibody/product/Millipore
    Average 90 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    anti bax antibody - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    Image Search Results