glyceraldehyde 3 phosphate dehydrogenase gapdh  (Merck KGaA)

 
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    Structured Review

    Merck KGaA glyceraldehyde 3 phosphate dehydrogenase gapdh
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Merck KGaA
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Hierarchical control of interleukin 13 (IL-13) signals in lung fibroblasts by STAT6 and SOX11
    Article Snippet: The antibodies (Abs) used in this study were against STAT6 (Santa Cruz Biotechnology), phosphorylated STAT6 (Tyr-641; Cell Signaling Technology), SOX11 (Merck Millipore), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Merck Millipore). .. In SOX11 overexpression experiments, pME18S encoding human SOX11 cDNA was transfected into HEK293T cells, which were harvested for Western blotting 24 h after transfection.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
    Article Snippet: Proteins were separated using an 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel and transferred to a polyvinyldine fluoride membrane (PVDF, Merck Millipore, Billerica, MA, USA). .. Then, the membranes were incubated with antibodies against GLUT1 (1:500 dilution, ab15309, Abcam, Cambridge, MA, USA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution, MAB374, Merck Millipore, Billerica, MA, USA).

    Generated:

    Article Title: A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies
    Article Snippet: Plasmids, antibodies and reagents Plasmids pRK5-TAOK1, pRK5-TAOK2, pRK5-TAOK2 (K57A) and pRK5-TAOK2 (amino acids 1–349) were generated as described previously [ , ] and subcloned into pCAGGS-IRES-EGFP or pCAGGS-IRES-Tomato vectors (gift from Dr. L. Gasparini, Italian Institute of Technology, Genoa, Italy). .. Commercially available mouse monoclonal antibodies were obtained to detect TAOK1 (BD), tau-pS202/T205/S208 (AT8, Thermo Fisher Scientific), total tau (Tau5, Thermo Fisher Scientific), synaptophysin (SP15, Enzo), Myc (9E10, Sigma-Aldrich) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Merck Millipore).

    Produced:

    Article Title: A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies
    Article Snippet: Tau-pT123, tau-pT427 and TAOK-pS181 affinity-purified rabbit antibodies and peptides were produced by Eurogentec. .. Commercially available mouse monoclonal antibodies were obtained to detect TAOK1 (BD), tau-pS202/T205/S208 (AT8, Thermo Fisher Scientific), total tau (Tau5, Thermo Fisher Scientific), synaptophysin (SP15, Enzo), Myc (9E10, Sigma-Aldrich) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Merck Millipore).

    Incubation:

    Article Title: Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
    Article Snippet: .. Then, the membranes were incubated with antibodies against GLUT1 (1:500 dilution, ab15309, Abcam, Cambridge, MA, USA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution, MAB374, Merck Millipore, Billerica, MA, USA). .. After washing with TBS-T, it was incubated with goat anti-rabbit or anti-mouse horseradish peroxidase conjugated secondary antibodies (1:30000 dilution, A16110 or 31430, Thermo Fisher, Waltham, MA, USA).

    Expressing:

    Article Title: A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies
    Article Snippet: PLenti-lox3.7 vectors expressing TAOK2-shRNA or non-targeting-shRNA and enhanced green fluorescent protein (EGFP) using an alternative promoter were a gift from Dr. S. Ultanir (Crick Institute, London, UK). .. Commercially available mouse monoclonal antibodies were obtained to detect TAOK1 (BD), tau-pS202/T205/S208 (AT8, Thermo Fisher Scientific), total tau (Tau5, Thermo Fisher Scientific), synaptophysin (SP15, Enzo), Myc (9E10, Sigma-Aldrich) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Merck Millipore).

    Protein Concentration:

    Article Title: Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
    Article Snippet: The protein concentration of supernatant was determined using bicinchoninic acid reagent (Thermo Fisher, Waltham, MA, USA). .. Then, the membranes were incubated with antibodies against GLUT1 (1:500 dilution, ab15309, Abcam, Cambridge, MA, USA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution, MAB374, Merck Millipore, Billerica, MA, USA).

    Western Blot:

    Article Title: Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status
    Article Snippet: .. Primary antibodies used for immunofluorescence (IF) and/or western blotting (WB) The following primary antibodies were used for immunofluorescence (IF) and/or western blotting (WB): mouse anti-lamin A [ab8980, Abcam, Paris, France (IF: 1/100, WB: 1/200)]; rabbit anti-lamin C [BP4505, Acris, Herford, Germany (IF: 1/100, WB: 1/230)]; mouse anti-lamin A/C [MAB3211, Merck Millipore, Massachusetts, United States (WB: 1/200)]; goat anti-lamin A/C [sc6215, Santa Cruz, Texas, United States (WB: 1/200)]; rabbit anti-lamin A/C [sc20681, Santa Cruz (WB: 1/200)]; rabbit anti-lamin B1 [ab16048, Abcam (WB: 1/250)]; glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [MAB374, Merck Millipore (WB: 1/40,000)]. .. For IF, the following isotype antibodies were used as negative controls: mouse IgG (015-000-003, Jackson ImmunoResearch, Suffolk, UK); and rabbit IgG (AB-105-C, R & D, Minnesota, United States).

    Article Title: Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET
    Article Snippet: Paragraph title: Western blotting ... Then, the membranes were incubated with antibodies against GLUT1 (1:500 dilution, ab15309, Abcam, Cambridge, MA, USA) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000 dilution, MAB374, Merck Millipore, Billerica, MA, USA).

    Article Title: Hierarchical control of interleukin 13 (IL-13) signals in lung fibroblasts by STAT6 and SOX11
    Article Snippet: Paragraph title: Western blotting ... The antibodies (Abs) used in this study were against STAT6 (Santa Cruz Biotechnology), phosphorylated STAT6 (Tyr-641; Cell Signaling Technology), SOX11 (Merck Millipore), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Merck Millipore).

    Article Title: The Novel Phosphatidylinositol-3-Kinase (PI3K) Inhibitor Alpelisib Effectively Inhibits Growth of PTEN-Haploinsufficient Lipoma Cells
    Article Snippet: Paragraph title: 4.5. Western Blot Analysis ... Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Merck KGaA, Darmstadt, Germany) was used as loading control.

    Affinity Purification:

    Article Title: A new TAO kinase inhibitor reduces tau phosphorylation at sites associated with neurodegeneration in human tauopathies
    Article Snippet: Tau-pT123, tau-pT427 and TAOK-pS181 affinity-purified rabbit antibodies and peptides were produced by Eurogentec. .. Commercially available mouse monoclonal antibodies were obtained to detect TAOK1 (BD), tau-pS202/T205/S208 (AT8, Thermo Fisher Scientific), total tau (Tau5, Thermo Fisher Scientific), synaptophysin (SP15, Enzo), Myc (9E10, Sigma-Aldrich) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Merck Millipore).

    Over Expression:

    Article Title: Hierarchical control of interleukin 13 (IL-13) signals in lung fibroblasts by STAT6 and SOX11
    Article Snippet: The antibodies (Abs) used in this study were against STAT6 (Santa Cruz Biotechnology), phosphorylated STAT6 (Tyr-641; Cell Signaling Technology), SOX11 (Merck Millipore), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Merck Millipore). .. In SOX11 overexpression experiments, pME18S encoding human SOX11 cDNA was transfected into HEK293T cells, which were harvested for Western blotting 24 h after transfection.

    Immunofluorescence:

    Article Title: Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status
    Article Snippet: .. Primary antibodies used for immunofluorescence (IF) and/or western blotting (WB) The following primary antibodies were used for immunofluorescence (IF) and/or western blotting (WB): mouse anti-lamin A [ab8980, Abcam, Paris, France (IF: 1/100, WB: 1/200)]; rabbit anti-lamin C [BP4505, Acris, Herford, Germany (IF: 1/100, WB: 1/230)]; mouse anti-lamin A/C [MAB3211, Merck Millipore, Massachusetts, United States (WB: 1/200)]; goat anti-lamin A/C [sc6215, Santa Cruz, Texas, United States (WB: 1/200)]; rabbit anti-lamin A/C [sc20681, Santa Cruz (WB: 1/200)]; rabbit anti-lamin B1 [ab16048, Abcam (WB: 1/250)]; glyceraldehyde 3-phosphate dehydrogenase (GAPDH) [MAB374, Merck Millipore (WB: 1/40,000)]. .. For IF, the following isotype antibodies were used as negative controls: mouse IgG (015-000-003, Jackson ImmunoResearch, Suffolk, UK); and rabbit IgG (AB-105-C, R & D, Minnesota, United States).

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    Merck KGaA anti bax
    Inhibition of endothelial cell conditioned medium (EC-CM) induced pro-apoptotic molecule expression by isoflurane. To examine the effect of pretreated isoflurane on neuronal cell survival and death under culture with EC-CM, we performed western blot analysis using <t>Bcl-2</t> and <t>Bax.</t> (B) The Bcl-2 protein was significantly upregulated in neuronal cells incubated with EC-CM of isoflurane, RAP, or isoflurane RAP treated groups in the tPA and OGD/R condition. (C) In contrast to Bcl-2, the protein levels of Bax was significantly downregulated in neuronal cells incubated with EC-CM of isoflurane, RAP or isoflurane + RAP-treated groups in the tPA and OGD/R condition. (E) Bcl-2 protein levels were significantly increased in neuronal cells incubated with EC-CM of isoflurane, MG-132, or isoflurane + MG-132-treated groups in the tPA and OGD/R condition. (F) Bax was significantly decreased in neuronal cells incubated with EC-CM of isoflurane, MG-132 and isoflurane + MG-132-treated groups in the tPA and OGD/R condition. * p
    Anti Bax, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 1 article reviews
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    91
    Merck KGaA mouse anti bax
    Reduction of apoptotic molecules following the inhibition of ASK1. (A) The levels of anti-apoptotic <t>(Bcl-2)</t> and pro-apoptotic <t>(Bax)</t> proteins were detected with Western blot analyses. (B) The relative optical density indicated that neuronal Bcl-2 expression was reduced after incubating the cells with EC-CM from the H/R group. After incubation with NQDI-1-treated EC-CM, the Bcl-2 level was increased ( n = 3). (C) The increased Bax level that was observed after incubating the cells with H/R-injured EC-CM was reduced after NQDI-1 treatment ( n = 3-4). (D) The level of apoptotic (caspase-3) protein was measured by Western blot analyses. (E) The graph showed the cleaved caspase-3 was increased in H/R-injured EC-CM, whereas ASK1-inhibited group exhibited lower cleaved caspase-3 level in neuronal cells ( n = 3). (F) Cell viability was detected with viability assays. The quantitative analyses show that neuronal cells were significantly dead after incubation with H/R-injured EC-CM; however, the cell viability was increased by treatment with ASK1 inhibitor ( n = 7). β-actin was used as an internal control. [Bars represent mean ± SEM, n = 3–4. Relative optical density (OD) of Bcl-2: H/R, 0. 79 ± 0.04; H/R+NQDI-1, 1.10 ± 0.05. OD of Bax: H/R, 1.27 ± 0.08; H/R+NQDI-1, 1.01 ± 0.04. OD of cleaved caspase-3: H/R, 1.17 ± 0.04; H/R+NQDI-1, 0.92 ± 0.05. * p
    Mouse Anti Bax, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti bax/product/Merck KGaA
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    98
    Merck KGaA gapdh
    The <t>p53</t> synthesis efficiency under different stress conditions in NIH3T3 cells. The cells were exposed for 16 hours to doxorubicin (0.1 μM), tunicamycin (1.2 μM) and thapsigargin (0.1 μM), or to an equivalent volume of DMSO, and then harvested. Endogenous p53 protein level was determined by western blot (a) or flow cytometry (b) using monoclonal 1C12 antibody. <t>GAPDH</t> was used as a loading control. The RT-PCR analysis of p53 mRNA and β-actin mRNA (as a control) was also conducted. For RT-PCR analysis, the samples were prepared using total RNA extracted from mouse fibroblasts treated with DMSO as a control, tunicamycin, thapsigargin and doxorubicin.
    Gapdh, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 98/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    gapdh - by Bioz Stars, 2020-04
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    Image Search Results


    Inhibition of endothelial cell conditioned medium (EC-CM) induced pro-apoptotic molecule expression by isoflurane. To examine the effect of pretreated isoflurane on neuronal cell survival and death under culture with EC-CM, we performed western blot analysis using Bcl-2 and Bax. (B) The Bcl-2 protein was significantly upregulated in neuronal cells incubated with EC-CM of isoflurane, RAP, or isoflurane RAP treated groups in the tPA and OGD/R condition. (C) In contrast to Bcl-2, the protein levels of Bax was significantly downregulated in neuronal cells incubated with EC-CM of isoflurane, RAP or isoflurane + RAP-treated groups in the tPA and OGD/R condition. (E) Bcl-2 protein levels were significantly increased in neuronal cells incubated with EC-CM of isoflurane, MG-132, or isoflurane + MG-132-treated groups in the tPA and OGD/R condition. (F) Bax was significantly decreased in neuronal cells incubated with EC-CM of isoflurane, MG-132 and isoflurane + MG-132-treated groups in the tPA and OGD/R condition. * p

    Journal: International Journal of Medical Sciences

    Article Title: Isoflurane preconditioning inhibits the effects of tissue-type plasminogen activator on brain endothelial cell in an in vitro model of ischemic stroke

    doi: 10.7150/ijms.18037

    Figure Lengend Snippet: Inhibition of endothelial cell conditioned medium (EC-CM) induced pro-apoptotic molecule expression by isoflurane. To examine the effect of pretreated isoflurane on neuronal cell survival and death under culture with EC-CM, we performed western blot analysis using Bcl-2 and Bax. (B) The Bcl-2 protein was significantly upregulated in neuronal cells incubated with EC-CM of isoflurane, RAP, or isoflurane RAP treated groups in the tPA and OGD/R condition. (C) In contrast to Bcl-2, the protein levels of Bax was significantly downregulated in neuronal cells incubated with EC-CM of isoflurane, RAP or isoflurane + RAP-treated groups in the tPA and OGD/R condition. (E) Bcl-2 protein levels were significantly increased in neuronal cells incubated with EC-CM of isoflurane, MG-132, or isoflurane + MG-132-treated groups in the tPA and OGD/R condition. (F) Bax was significantly decreased in neuronal cells incubated with EC-CM of isoflurane, MG-132 and isoflurane + MG-132-treated groups in the tPA and OGD/R condition. * p

    Article Snippet: After blocking with 5% bovine serum albumin (BSA), membranes were incubated with the anti-LRP (1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-NF-κB p65 (1:500, Santa Cruz Biotechnology), anti-Cox-2 (1:500, Santa Cruz Biotechnology), anti-Bax (1:1000, Merck Millipore, Bedford, MA, USA), anti-Bcl-2 (1:1000, Abcam, Cambridge, UK) primary antibodies respectively.

    Techniques: Inhibition, Expressing, Western Blot, Incubation

    Bufalin alters the apoptosis-related protein levels in A375.S2 cells. A total of 1 × 10 6 A375.S2 cells in 6-well plates were treated with 450 nM bufalin for 0, 6, 12, 24, and 48 h. Cells were harvested from each sample and associated proteins were measured by using SDS-PAGE and Western blotting as described in Section 2 . The protein levels of caspase-3, caspase-8, and caspase-9 (a), cytochrome c , AIF, Endo G, Bcl-X, and Bax (b), and Fas, FasL, and GRP78 (c) expressions were examined. β -Actin was used as an internal control to ensure equal loading.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Triggering Apoptotic Death of Human Malignant Melanoma A375.S2 Cells by Bufalin: Involvement of Caspase Cascade-Dependent and Independent Mitochondrial Signaling Pathways

    doi: 10.1155/2012/591241

    Figure Lengend Snippet: Bufalin alters the apoptosis-related protein levels in A375.S2 cells. A total of 1 × 10 6 A375.S2 cells in 6-well plates were treated with 450 nM bufalin for 0, 6, 12, 24, and 48 h. Cells were harvested from each sample and associated proteins were measured by using SDS-PAGE and Western blotting as described in Section 2 . The protein levels of caspase-3, caspase-8, and caspase-9 (a), cytochrome c , AIF, Endo G, Bcl-X, and Bax (b), and Fas, FasL, and GRP78 (c) expressions were examined. β -Actin was used as an internal control to ensure equal loading.

    Article Snippet: Anti-endonuclease G (Endo G) (Cat. AB3639) and anti-Bax (Cat. 04-434) were bought from Merck Millipore (Billerica,MA,USA).

    Techniques: SDS Page, Western Blot

    Reduction of apoptotic molecules following the inhibition of ASK1. (A) The levels of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) proteins were detected with Western blot analyses. (B) The relative optical density indicated that neuronal Bcl-2 expression was reduced after incubating the cells with EC-CM from the H/R group. After incubation with NQDI-1-treated EC-CM, the Bcl-2 level was increased ( n = 3). (C) The increased Bax level that was observed after incubating the cells with H/R-injured EC-CM was reduced after NQDI-1 treatment ( n = 3-4). (D) The level of apoptotic (caspase-3) protein was measured by Western blot analyses. (E) The graph showed the cleaved caspase-3 was increased in H/R-injured EC-CM, whereas ASK1-inhibited group exhibited lower cleaved caspase-3 level in neuronal cells ( n = 3). (F) Cell viability was detected with viability assays. The quantitative analyses show that neuronal cells were significantly dead after incubation with H/R-injured EC-CM; however, the cell viability was increased by treatment with ASK1 inhibitor ( n = 7). β-actin was used as an internal control. [Bars represent mean ± SEM, n = 3–4. Relative optical density (OD) of Bcl-2: H/R, 0. 79 ± 0.04; H/R+NQDI-1, 1.10 ± 0.05. OD of Bax: H/R, 1.27 ± 0.08; H/R+NQDI-1, 1.01 ± 0.04. OD of cleaved caspase-3: H/R, 1.17 ± 0.04; H/R+NQDI-1, 0.92 ± 0.05. * p

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Blockade of Apoptosis Signal-Regulating Kinase 1 Attenuates Matrix Metalloproteinase 9 Activity in Brain Endothelial Cells and the Subsequent Apoptosis in Neurons after Ischemic Injury

    doi: 10.3389/fncel.2016.00213

    Figure Lengend Snippet: Reduction of apoptotic molecules following the inhibition of ASK1. (A) The levels of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax) proteins were detected with Western blot analyses. (B) The relative optical density indicated that neuronal Bcl-2 expression was reduced after incubating the cells with EC-CM from the H/R group. After incubation with NQDI-1-treated EC-CM, the Bcl-2 level was increased ( n = 3). (C) The increased Bax level that was observed after incubating the cells with H/R-injured EC-CM was reduced after NQDI-1 treatment ( n = 3-4). (D) The level of apoptotic (caspase-3) protein was measured by Western blot analyses. (E) The graph showed the cleaved caspase-3 was increased in H/R-injured EC-CM, whereas ASK1-inhibited group exhibited lower cleaved caspase-3 level in neuronal cells ( n = 3). (F) Cell viability was detected with viability assays. The quantitative analyses show that neuronal cells were significantly dead after incubation with H/R-injured EC-CM; however, the cell viability was increased by treatment with ASK1 inhibitor ( n = 7). β-actin was used as an internal control. [Bars represent mean ± SEM, n = 3–4. Relative optical density (OD) of Bcl-2: H/R, 0. 79 ± 0.04; H/R+NQDI-1, 1.10 ± 0.05. OD of Bax: H/R, 1.27 ± 0.08; H/R+NQDI-1, 1.01 ± 0.04. OD of cleaved caspase-3: H/R, 1.17 ± 0.04; H/R+NQDI-1, 0.92 ± 0.05. * p

    Article Snippet: Membranes were blocked with 5% BSA and incubated in the rabbit anti-phosphatidylinositol 3-kinase (Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-pAkt (1:1000, Cell signaling, Danvers, MA, USA), rabbit anti-Akt (1:1000, Cell signaling), rabbit anti-nuclear factor erythroid 2 [NF-E2]-related factor 2 (1:1000, Santa Cruz Biotechnology), rabbit anti- HO-1 (1:1000, Abcam, Cambridge, UK), rabbit anti-Bcl-2 (1:1000, Abcam), mouse anti-Bax (1:1000, Merck Millipore), goat anti-cyclooxygenase-2 (1:500, Santa Cruz Biotechnology), rabbit anti-cleaved caspase-3 (1:1000, Santa Cruz Biotechnology), and rabbit anti-caspase-3 (1:1000, Merck Millipore) which is diluted in 2% BSA.

    Techniques: Inhibition, Western Blot, Expressing, Incubation

    The p53 synthesis efficiency under different stress conditions in NIH3T3 cells. The cells were exposed for 16 hours to doxorubicin (0.1 μM), tunicamycin (1.2 μM) and thapsigargin (0.1 μM), or to an equivalent volume of DMSO, and then harvested. Endogenous p53 protein level was determined by western blot (a) or flow cytometry (b) using monoclonal 1C12 antibody. GAPDH was used as a loading control. The RT-PCR analysis of p53 mRNA and β-actin mRNA (as a control) was also conducted. For RT-PCR analysis, the samples were prepared using total RNA extracted from mouse fibroblasts treated with DMSO as a control, tunicamycin, thapsigargin and doxorubicin.

    Journal: RNA Biology

    Article Title: Length and secondary structure of the 5′ non-coding regions of mouse p53 mRNA transcripts - mouse as a model organism for p53 gene expression studies

    doi: 10.1080/15476286.2018.1556084

    Figure Lengend Snippet: The p53 synthesis efficiency under different stress conditions in NIH3T3 cells. The cells were exposed for 16 hours to doxorubicin (0.1 μM), tunicamycin (1.2 μM) and thapsigargin (0.1 μM), or to an equivalent volume of DMSO, and then harvested. Endogenous p53 protein level was determined by western blot (a) or flow cytometry (b) using monoclonal 1C12 antibody. GAPDH was used as a loading control. The RT-PCR analysis of p53 mRNA and β-actin mRNA (as a control) was also conducted. For RT-PCR analysis, the samples were prepared using total RNA extracted from mouse fibroblasts treated with DMSO as a control, tunicamycin, thapsigargin and doxorubicin.

    Article Snippet: The blot was probed with mouse monoclonal antibodies against p53 (1C12; Cell Signaling), and GAPDH (Anti-Glyceraldehyde-3-Phosphate Dehydrogenase, clone 6C5ʹ, Merck Millipore).

    Techniques: Western Blot, Flow Cytometry, Cytometry, Reverse Transcription Polymerase Chain Reaction