glyceraldehyde 3 phosphate dehydrogenase gapdh  (Abcam)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Abcam glyceraldehyde 3 phosphate dehydrogenase gapdh
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Abcam
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-02
    99/100 stars

    Images

    Related Articles

    Transduction:

    Article Title: Polyubiquitin Binding to Optineurin Is Required for Optimal Activation of TANK-binding Kinase 1 and Production of Interferon β
    Article Snippet: Antibodies against mouse OPTN (sheep number S308C, second bleed) or human OPTN (sheep number S009C, third bleed), human TBK1 (sheep S041C, second bleed), and the C-terminal peptide of mouse IKKϵ (NRLIERLHRVPSAPDV) (sheep S277C, second bleed), which were used to immunoprecipitate mouse OPTN, TBK1, and IKKϵ, respectively, were generated by the Division of Signal Transduction Therapy, the Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee, UK. .. Anti-OPTN antibody (for immunoblotting) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Abcam (Cambridge, UK).

    Synthesized:

    Article Title: miRNA-129/FBW7/NF-κB, a Novel Regulatory Pathway in Inflammatory Bowel Disease
    Article Snippet: Materials and Reagents Antibodies against FBW7, ubiquitin, GFP, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Abcam (MA, USA). .. IκBα, p-IκBα, p65, HA, myc, and immunoglobulin G (IgG) antibodies were purchased from Cell Signaling Technology (MA, USA). miR-129) mimics, miR-363 mimics, miR-223 mimics, miR-27a mimics, mimics negative control, miR-129 inhibitor, inhibitor negative control, FBW7 siRNA, negative siRNA, and miR-129 and U6 primers were synthesized from RiboBio (Guangzhou, China).

    Construct:

    Article Title: miRNA-129/FBW7/NF-κB, a Novel Regulatory Pathway in Inflammatory Bowel Disease
    Article Snippet: Materials and Reagents Antibodies against FBW7, ubiquitin, GFP, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Abcam (MA, USA). .. Mammalian expression plasmids for myc-tagged ubiquitin and HA-tagged IκBα were constructed by BioVector NTCC (Beijing, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Optic Nerve Lipidomics Reveal Impaired Glucosylsphingosine Lipids Pathway in Glaucoma
    Article Snippet: Paragraph title: Western Blot Analysis and ELISA Analysis ... The primary antibodies against glucocerebrosidase (GBA) (rabbit, 1:5000, Abcam, Inc., Cambridge, UK), nonlysosomal glucocerebrosidase (GBA2) (rabbit, 1:500, Abcam, Inc.), acylsphingosine amidohydrolase (ASAH1) (rabbit, 1:5000, Abcam, Inc.), ASAH2 (rabbit, 1:5000, Abcam, Inc.), α-synuclein (sheep, 1:500, Abcam, Inc.), ceramide glucosyltransferase (UGCG) (rabbit polyclonal, 1:5000, Abcam, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (rabbit, 1:1000, Abcam, Inc.) were used for protein detection.

    Modification:

    Article Title: β-elemene induced anticancer effect in bladder cancer through upregulation of PTEN and suppression of AKT phosphorylation
    Article Snippet: Antibodies against PTEN, pAKT, AKT, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and peroxidase-labelled anti-rabbit IgG were purchased from Abcam (Cambridge, UK). .. The fetal bovine serum (FBS), Roswell Park Memorial Institute-1640 (RPMI-1640) and Dulbecco's modified Eagle's medium (DMEM) were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA).

    Incubation:

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam). .. After thoroughly washing for 60 min with TBST 0.1% (Bio-Rad, Hercules, CA), the membranes were incubated with HRP-conjugated anti-mouse secondary antibodies (1:5000, Abcam) for 1 h at room temperature.

    Article Title: FOXM1: A potential indicator to predict lymphatic metastatic recurrence in stage IIA esophageal squamous cell carcinoma
    Article Snippet: .. Membranes were incubated overnight at 4°C with primary antibodies anti‐FOXM1 (1:500) and anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1: 5000; Abcam, Cambridge, MA, USA). .. After washing, the membranes were incubated with corresponding secondary antibodies (horseradish peroxidase‐conjugated goat anti‐rabbit antibodies, 1: 5000; ZSGB Biotech, Beijing, China) for one hour at room temperature.

    Proliferation Assay:

    Article Title: The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A
    Article Snippet: L-lactate assay kits were obtained from Eton Bioscience (San Diego, CA, USA), and water-soluble tetrazolium (WST) proliferation assay kits from GBiosciences (St. Louis, MO, USA). .. Primary antibodies monocarboxylate transporter 1(MCT1), monocarboxylate transporter 3 (MCT3), monocarboxylate transporter 4 (MCT4), Basigin (CD147), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), secondary antibody and chemiluminescence reagent, were provided by Abcam (Cambridge, MA, USA).

    Expressing:

    Article Title: MicroRNA-940 restricts the expression of metastasis-associated gene MACC1 and enhances the antitumor effect of Anlotinib on colorectal cancer
    Article Snippet: .. The expression of MACC1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the loading control, was detected by their antibodies (anti-MACC1 antibody [Cat. No. ab106579, Abcam Corporation, Cambridge, CB4 0FL, UK]; anti-GAPDH antibody [Cat. No. ab8245], Abcam Corporation, Cambridge, CB4 0FL, UK). .. In vivo antitumor effect of Anlotinib Performance of animal experiments was reviewed and approved by the Institutional Animal Care and Use Committee of the Beijing Tiantan Hospital.

    Article Title: miRNA-129/FBW7/NF-κB, a Novel Regulatory Pathway in Inflammatory Bowel Disease
    Article Snippet: Materials and Reagents Antibodies against FBW7, ubiquitin, GFP, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Abcam (MA, USA). .. Mammalian expression plasmids for myc-tagged ubiquitin and HA-tagged IκBα were constructed by BioVector NTCC (Beijing, China).

    Article Title: Biological Characterization of Chlamydia trachomatis Plasticity Zone MACPF Domain Family Protein CT153 ▿ Plasticity Zone MACPF Domain Family Protein CT153 ▿ †
    Article Snippet: Paragraph title: Temporal kinetics of CT153 processing and expression in infected cells. ... Samples were subjected to SDS-PAGE and Western blotting using anti-CT153, anti-MOMP (B-B5b), or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, Cambridge, MA).

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam). .. GAPDH protein expression from parallel runs was used for the normalization of results.

    BIA-KA:

    Article Title: Iron overload induced death of osteoblasts in vitro: involvement of the mitochondrial apoptotic pathway
    Article Snippet: Primary antibodies against cytochrome c, bcl-2, bax, cleaved Caspase-3, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and beta-actin (β -actin) were purchased from Abcam (Cambridge, UK). .. Cell Mitochondria Isolation Kit, Enhanced Chemiluminescence detection kit, Western and immunoprecipitation (IP) Cell Lysis Kit, Bicinchoninic Acid Protein (BCA) Protein Assay Kit, and 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanineiodide (JC-1) were purchased from Beyotime (China).

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine protein concentration. .. Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam).

    Acrylamide Gel Assay:

    Article Title: Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production
    Article Snippet: Each sample was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 12% acrylamide gel and transferred electrophoretically to a PVDF membrane. .. The primary antibodies used were anti-Flag (Sigma), anti-HIV-1 p24 (Santacruz), anti-Fluc (Cal Biochem, USA), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-beta-actin (abcam), anti-RFP polyclonal, and anti-GFP monoclonal (Clontech/Takara Bio Co, USA).

    Western Blot:

    Article Title: miR-130 Suppresses Adipogenesis by Inhibiting Peroxisome Proliferator-Activated Receptor ? Expression ▿
    Article Snippet: Paragraph title: Western blot analysis. ... Incubations with primary antibodies to detect PPARγ or GFP (Santa Cruz Biotech), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or β-actin (Abcam), were followed by incubations with the appropriate secondary antibodies conjugated with horseradish peroxidase (HRP) and by detection using enhanced luminescence (GE Healthcare).

    Article Title: MicroRNA-940 restricts the expression of metastasis-associated gene MACC1 and enhances the antitumor effect of Anlotinib on colorectal cancer
    Article Snippet: Paragraph title: Western blot experiments ... The expression of MACC1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the loading control, was detected by their antibodies (anti-MACC1 antibody [Cat. No. ab106579, Abcam Corporation, Cambridge, CB4 0FL, UK]; anti-GAPDH antibody [Cat. No. ab8245], Abcam Corporation, Cambridge, CB4 0FL, UK).

    Article Title: Optic Nerve Lipidomics Reveal Impaired Glucosylsphingosine Lipids Pathway in Glaucoma
    Article Snippet: Paragraph title: Western Blot Analysis and ELISA Analysis ... The primary antibodies against glucocerebrosidase (GBA) (rabbit, 1:5000, Abcam, Inc., Cambridge, UK), nonlysosomal glucocerebrosidase (GBA2) (rabbit, 1:500, Abcam, Inc.), acylsphingosine amidohydrolase (ASAH1) (rabbit, 1:5000, Abcam, Inc.), ASAH2 (rabbit, 1:5000, Abcam, Inc.), α-synuclein (sheep, 1:500, Abcam, Inc.), ceramide glucosyltransferase (UGCG) (rabbit polyclonal, 1:5000, Abcam, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (rabbit, 1:1000, Abcam, Inc.) were used for protein detection.

    Article Title: Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model
    Article Snippet: Paragraph title: Western blot analysis ... The membranes were blocked with 5% milk in Tris-buffered saline and Tween-20 buffer (10 mM Tris, 100 mM NaCl, and 0.1% Tween-20, pH 8.0) and subsequently probed with primary antibodies against heat-shock protein 70 (HSP-70), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), IL-1β, NF-κB, TNF-α, MMP3/7/9 (1:1,000; Abcam, Cambridge, MA, USA), inducible nitric oxide synthase (iNOS, 1:1,000 dilution, BD Transduction Laboratory, San Diego, CA, USA), p-Erk1/2, COX-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4℃.

    Article Title: Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production
    Article Snippet: Paragraph title: Western blot analysis ... The primary antibodies used were anti-Flag (Sigma), anti-HIV-1 p24 (Santacruz), anti-Fluc (Cal Biochem, USA), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-beta-actin (abcam), anti-RFP polyclonal, and anti-GFP monoclonal (Clontech/Takara Bio Co, USA).

    Article Title: Biological Characterization of Chlamydia trachomatis Plasticity Zone MACPF Domain Family Protein CT153 ▿ Plasticity Zone MACPF Domain Family Protein CT153 ▿ †
    Article Snippet: .. Samples were subjected to SDS-PAGE and Western blotting using anti-CT153, anti-MOMP (B-B5b), or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, Cambridge, MA). .. C. trachomatis serovar L2-infected L929 suspension cultures were harvested by centrifugation at 0, 12, 24, 30, and 36 h postinfection and lysed by Dounce homogenization, and cellular fractionation was performed as previously described ( , , ).

    Article Title: Human fetal skin-derived stem cell secretome enhances radiation-induced skin injury therapeutic effects by promoting angiogenesis
    Article Snippet: .. Resolved proteins were then electro blotted onto nitrocellulose membranes and probed with antibody against Col1A2, Col3A1, TGF-β1, TGF-β3, Ang-1, Ang-2, VEGF, PLGF, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000 dilution, Abcam, Cambridge, UK) overnight at 4 °C. .. These were followed by secondary antibodies HRP-conjugated goat anti-rabbit IgG (1:1000 dilution, Abcam, Cambridge, UK) and visualized by chemiluminescent detection according to the manufacturer’s instructions (Immobilon western chemiluminescent HRP substrate, Millipore).

    Article Title: Iron overload induced death of osteoblasts in vitro: involvement of the mitochondrial apoptotic pathway
    Article Snippet: Primary antibodies against cytochrome c, bcl-2, bax, cleaved Caspase-3, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and beta-actin (β -actin) were purchased from Abcam (Cambridge, UK). .. Cell Mitochondria Isolation Kit, Enhanced Chemiluminescence detection kit, Western and immunoprecipitation (IP) Cell Lysis Kit, Bicinchoninic Acid Protein (BCA) Protein Assay Kit, and 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanineiodide (JC-1) were purchased from Beyotime (China).

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: Paragraph title: Western blot ... Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam).

    Article Title: FOXM1: A potential indicator to predict lymphatic metastatic recurrence in stage IIA esophageal squamous cell carcinoma
    Article Snippet: Paragraph title: Western blot analysis ... Membranes were incubated overnight at 4°C with primary antibodies anti‐FOXM1 (1:500) and anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1: 5000; Abcam, Cambridge, MA, USA).

    Immunoprecipitation:

    Article Title: Iron overload induced death of osteoblasts in vitro: involvement of the mitochondrial apoptotic pathway
    Article Snippet: Primary antibodies against cytochrome c, bcl-2, bax, cleaved Caspase-3, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and beta-actin (β -actin) were purchased from Abcam (Cambridge, UK). .. Cell Mitochondria Isolation Kit, Enhanced Chemiluminescence detection kit, Western and immunoprecipitation (IP) Cell Lysis Kit, Bicinchoninic Acid Protein (BCA) Protein Assay Kit, and 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanineiodide (JC-1) were purchased from Beyotime (China).

    Transferring:

    Article Title: The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A
    Article Snippet: Primary antibodies monocarboxylate transporter 1(MCT1), monocarboxylate transporter 3 (MCT3), monocarboxylate transporter 4 (MCT4), Basigin (CD147), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), secondary antibody and chemiluminescence reagent, were provided by Abcam (Cambridge, MA, USA). .. Bio- Rad (Hercules, CA, USA) supplied running and transferring buffers, standard protein ladder, Laemmli sample buffer, and nitrocellulose.

    Infection:

    Article Title: Biological Characterization of Chlamydia trachomatis Plasticity Zone MACPF Domain Family Protein CT153 ▿ Plasticity Zone MACPF Domain Family Protein CT153 ▿ †
    Article Snippet: Paragraph title: Temporal kinetics of CT153 processing and expression in infected cells. ... Samples were subjected to SDS-PAGE and Western blotting using anti-CT153, anti-MOMP (B-B5b), or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, Cambridge, MA).

    Immunoperoxidase Staining:

    Article Title: Tumor Cells from Ultrasonic Aspirations of Astrocytomas Migrate and Form Spheres with Radial Outgrowth
    Article Snippet: Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for immunoblotting and anti-GFAP for immunoblotting and immunofluoresence were obtained from Abcam, Inc. (Cambridge, MA, USA). .. Anti-GFAP for immunoperoxidase staining was obtained from DakoCytomation, Inc. (Carpinteria, CA, USA).

    Generated:

    Article Title: Polyubiquitin Binding to Optineurin Is Required for Optimal Activation of TANK-binding Kinase 1 and Production of Interferon β
    Article Snippet: Antibodies against mouse OPTN (sheep number S308C, second bleed) or human OPTN (sheep number S009C, third bleed), human TBK1 (sheep S041C, second bleed), and the C-terminal peptide of mouse IKKϵ (NRLIERLHRVPSAPDV) (sheep S277C, second bleed), which were used to immunoprecipitate mouse OPTN, TBK1, and IKKϵ, respectively, were generated by the Division of Signal Transduction Therapy, the Medical Research Council Protein Phosphorylation Unit, University of Dundee, Dundee, UK. .. Anti-OPTN antibody (for immunoblotting) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Abcam (Cambridge, UK).

    Imaging:

    Article Title: Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model
    Article Snippet: The membranes were blocked with 5% milk in Tris-buffered saline and Tween-20 buffer (10 mM Tris, 100 mM NaCl, and 0.1% Tween-20, pH 8.0) and subsequently probed with primary antibodies against heat-shock protein 70 (HSP-70), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), IL-1β, NF-κB, TNF-α, MMP3/7/9 (1:1,000; Abcam, Cambridge, MA, USA), inducible nitric oxide synthase (iNOS, 1:1,000 dilution, BD Transduction Laboratory, San Diego, CA, USA), p-Erk1/2, COX-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4℃. .. The protein bands were detected using the EZ-Capture ST imaging system (Atto, Tokyo, Japan).

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam). .. After re-washing the membranes, immunodetection and visualization of proteins were performed on a ChemiDoc MP Imaging System (Bio-Rad) using chemiluminescent substrate detection reagent (ECL, Bio-Rad).

    Protein Concentration:

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine protein concentration. .. Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam).

    Binding Assay:

    Article Title: FOXM1: A potential indicator to predict lymphatic metastatic recurrence in stage IIA esophageal squamous cell carcinoma
    Article Snippet: Briefly, 5% non‐fat dry milk was used to block the non‐specific binding. .. Membranes were incubated overnight at 4°C with primary antibodies anti‐FOXM1 (1:500) and anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1: 5000; Abcam, Cambridge, MA, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Optic Nerve Lipidomics Reveal Impaired Glucosylsphingosine Lipids Pathway in Glaucoma
    Article Snippet: Equal amounts (20 μg) of proteins per sample were fractionated on a 4% to 15% sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE; Invitrogen, Carlsbad, CA, USA) and transferred onto a polyvinylidene fluoride (PVDF) membrane using precut blotting sandwiches (Ready Gel, catalog no. 162-0219; Bio-Rad, Hercules, CA, USA). .. The primary antibodies against glucocerebrosidase (GBA) (rabbit, 1:5000, Abcam, Inc., Cambridge, UK), nonlysosomal glucocerebrosidase (GBA2) (rabbit, 1:500, Abcam, Inc.), acylsphingosine amidohydrolase (ASAH1) (rabbit, 1:5000, Abcam, Inc.), ASAH2 (rabbit, 1:5000, Abcam, Inc.), α-synuclein (sheep, 1:500, Abcam, Inc.), ceramide glucosyltransferase (UGCG) (rabbit polyclonal, 1:5000, Abcam, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (rabbit, 1:1000, Abcam, Inc.) were used for protein detection.

    Article Title: Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production
    Article Snippet: Each sample was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 12% acrylamide gel and transferred electrophoretically to a PVDF membrane. .. The primary antibodies used were anti-Flag (Sigma), anti-HIV-1 p24 (Santacruz), anti-Fluc (Cal Biochem, USA), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-beta-actin (abcam), anti-RFP polyclonal, and anti-GFP monoclonal (Clontech/Takara Bio Co, USA).

    Article Title: FOXM1: A potential indicator to predict lymphatic metastatic recurrence in stage IIA esophageal squamous cell carcinoma
    Article Snippet: Equal amounts of protein (40 μg) were separated by 10% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membrane filters (Millipore, Billerica, MA, USA). .. Membranes were incubated overnight at 4°C with primary antibodies anti‐FOXM1 (1:500) and anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1: 5000; Abcam, Cambridge, MA, USA).

    Isolation:

    Article Title: Iron overload induced death of osteoblasts in vitro: involvement of the mitochondrial apoptotic pathway
    Article Snippet: Primary antibodies against cytochrome c, bcl-2, bax, cleaved Caspase-3, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and beta-actin (β -actin) were purchased from Abcam (Cambridge, UK). .. Cell Mitochondria Isolation Kit, Enhanced Chemiluminescence detection kit, Western and immunoprecipitation (IP) Cell Lysis Kit, Bicinchoninic Acid Protein (BCA) Protein Assay Kit, and 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanineiodide (JC-1) were purchased from Beyotime (China).

    Caspase-3 Assay:

    Article Title: The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A
    Article Snippet: EnzChek® Caspase-3 Assay were purchased from Life Technologies Inc., (Grand Island, NY, USA). .. Primary antibodies monocarboxylate transporter 1(MCT1), monocarboxylate transporter 3 (MCT3), monocarboxylate transporter 4 (MCT4), Basigin (CD147), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), secondary antibody and chemiluminescence reagent, were provided by Abcam (Cambridge, MA, USA).

    Immunodetection:

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam). .. After re-washing the membranes, immunodetection and visualization of proteins were performed on a ChemiDoc MP Imaging System (Bio-Rad) using chemiluminescent substrate detection reagent (ECL, Bio-Rad).

    Blocking Assay:

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: Membranes were blocked in Odyssey Blocking Buffer (TBS, LI-COR, Lincoln, NE) for 3 h at room temperature. .. Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam).

    Article Title: FOXM1: A potential indicator to predict lymphatic metastatic recurrence in stage IIA esophageal squamous cell carcinoma
    Article Snippet: Briefly, 5% non‐fat dry milk was used to block the non‐specific binding. .. Membranes were incubated overnight at 4°C with primary antibodies anti‐FOXM1 (1:500) and anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1: 5000; Abcam, Cambridge, MA, USA).

    IA:

    Article Title: The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A
    Article Snippet: The plants were originally obtained from several sources including Frontier Natural Products Coop (Norway, IA, USA), Monterey Bay Spice Company (Watsonville, CA, USA), Mountain Rose, Herbs (Eugene, OR, USA), Mayway Traditional Chinese Herbs (Oakland, CA, USA), Kalyx Natural Marketplace (Camden, NY, USA), Futureceuticals (Momence, IL, USA), Organic Fruit Vegetable Markets and Florida Food Products Inc. (Eustis, FL, USA). .. Primary antibodies monocarboxylate transporter 1(MCT1), monocarboxylate transporter 3 (MCT3), monocarboxylate transporter 4 (MCT4), Basigin (CD147), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), secondary antibody and chemiluminescence reagent, were provided by Abcam (Cambridge, MA, USA).

    SDS Page:

    Article Title: MicroRNA-940 restricts the expression of metastasis-associated gene MACC1 and enhances the antitumor effect of Anlotinib on colorectal cancer
    Article Snippet: Protein bands in SDS-PAGE gel were trans-printed into PVDF films and films were blocked by 5% BSA at 37°C for 2 hrs. .. The expression of MACC1 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the loading control, was detected by their antibodies (anti-MACC1 antibody [Cat. No. ab106579, Abcam Corporation, Cambridge, CB4 0FL, UK]; anti-GAPDH antibody [Cat. No. ab8245], Abcam Corporation, Cambridge, CB4 0FL, UK).

    Article Title: Optic Nerve Lipidomics Reveal Impaired Glucosylsphingosine Lipids Pathway in Glaucoma
    Article Snippet: Equal amounts (20 μg) of proteins per sample were fractionated on a 4% to 15% sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE; Invitrogen, Carlsbad, CA, USA) and transferred onto a polyvinylidene fluoride (PVDF) membrane using precut blotting sandwiches (Ready Gel, catalog no. 162-0219; Bio-Rad, Hercules, CA, USA). .. The primary antibodies against glucocerebrosidase (GBA) (rabbit, 1:5000, Abcam, Inc., Cambridge, UK), nonlysosomal glucocerebrosidase (GBA2) (rabbit, 1:500, Abcam, Inc.), acylsphingosine amidohydrolase (ASAH1) (rabbit, 1:5000, Abcam, Inc.), ASAH2 (rabbit, 1:5000, Abcam, Inc.), α-synuclein (sheep, 1:500, Abcam, Inc.), ceramide glucosyltransferase (UGCG) (rabbit polyclonal, 1:5000, Abcam, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (rabbit, 1:1000, Abcam, Inc.) were used for protein detection.

    Article Title: Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model
    Article Snippet: Aliquots of 200 µg of protein extract were separated by 10%–15% sodium dodecyl sulfate polyacrylamide gel electrophorese (SDS-PAGE; Bio-Rad) and transferred to nitrocellulose membranes (Bio-Rad). .. The membranes were blocked with 5% milk in Tris-buffered saline and Tween-20 buffer (10 mM Tris, 100 mM NaCl, and 0.1% Tween-20, pH 8.0) and subsequently probed with primary antibodies against heat-shock protein 70 (HSP-70), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), IL-1β, NF-κB, TNF-α, MMP3/7/9 (1:1,000; Abcam, Cambridge, MA, USA), inducible nitric oxide synthase (iNOS, 1:1,000 dilution, BD Transduction Laboratory, San Diego, CA, USA), p-Erk1/2, COX-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4℃.

    Article Title: Understanding of the functional role(s) of the Activating Transcription Factor 4(ATF4) in HIV regulation and production
    Article Snippet: Each sample was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 12% acrylamide gel and transferred electrophoretically to a PVDF membrane. .. The primary antibodies used were anti-Flag (Sigma), anti-HIV-1 p24 (Santacruz), anti-Fluc (Cal Biochem, USA), anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), anti-beta-actin (abcam), anti-RFP polyclonal, and anti-GFP monoclonal (Clontech/Takara Bio Co, USA).

    Article Title: Biological Characterization of Chlamydia trachomatis Plasticity Zone MACPF Domain Family Protein CT153 ▿ Plasticity Zone MACPF Domain Family Protein CT153 ▿ †
    Article Snippet: .. Samples were subjected to SDS-PAGE and Western blotting using anti-CT153, anti-MOMP (B-B5b), or anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, Cambridge, MA). .. C. trachomatis serovar L2-infected L929 suspension cultures were harvested by centrifugation at 0, 12, 24, 30, and 36 h postinfection and lysed by Dounce homogenization, and cellular fractionation was performed as previously described ( , , ).

    Electrophoresis:

    Article Title: miR-130 Suppresses Adipogenesis by Inhibiting Peroxisome Proliferator-Activated Receptor ? Expression ▿
    Article Snippet: Whole-cell lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer, separated by electrophoresis in SDS-containing polyacrylamide gels, and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. Incubations with primary antibodies to detect PPARγ or GFP (Santa Cruz Biotech), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or β-actin (Abcam), were followed by incubations with the appropriate secondary antibodies conjugated with horseradish peroxidase (HRP) and by detection using enhanced luminescence (GE Healthcare).

    Article Title: Optic Nerve Lipidomics Reveal Impaired Glucosylsphingosine Lipids Pathway in Glaucoma
    Article Snippet: Electrophoresis was performed using a constant 70 V for 90 minutes. .. The primary antibodies against glucocerebrosidase (GBA) (rabbit, 1:5000, Abcam, Inc., Cambridge, UK), nonlysosomal glucocerebrosidase (GBA2) (rabbit, 1:500, Abcam, Inc.), acylsphingosine amidohydrolase (ASAH1) (rabbit, 1:5000, Abcam, Inc.), ASAH2 (rabbit, 1:5000, Abcam, Inc.), α-synuclein (sheep, 1:500, Abcam, Inc.), ceramide glucosyltransferase (UGCG) (rabbit polyclonal, 1:5000, Abcam, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (rabbit, 1:1000, Abcam, Inc.) were used for protein detection.

    Article Title: Adipose tissue, but not skeletal muscle, sirtuin 1 expression is decreased in obesity and related to insulin sensitivity
    Article Snippet: After electrophoresis proteins were transferred from the gel onto PVDF (polyvinylidene fluoride) membranes under wet conditions (30 V overnight at 4 °C). .. Primary antibodies were carried out overnight at 4 °C with anti-SIRT1 (1:500, Abcam, Cambridge, UK) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:2000, Abcam).

    Negative Control:

    Article Title: miRNA-129/FBW7/NF-κB, a Novel Regulatory Pathway in Inflammatory Bowel Disease
    Article Snippet: Materials and Reagents Antibodies against FBW7, ubiquitin, GFP, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were obtained from Abcam (MA, USA). .. IκBα, p-IκBα, p65, HA, myc, and immunoglobulin G (IgG) antibodies were purchased from Cell Signaling Technology (MA, USA). miR-129) mimics, miR-363 mimics, miR-223 mimics, miR-27a mimics, mimics negative control, miR-129 inhibitor, inhibitor negative control, FBW7 siRNA, negative siRNA, and miR-129 and U6 primers were synthesized from RiboBio (Guangzhou, China).

    Radio Immunoprecipitation:

    Article Title: miR-130 Suppresses Adipogenesis by Inhibiting Peroxisome Proliferator-Activated Receptor ? Expression ▿
    Article Snippet: Whole-cell lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer, separated by electrophoresis in SDS-containing polyacrylamide gels, and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). .. Incubations with primary antibodies to detect PPARγ or GFP (Santa Cruz Biotech), or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) or β-actin (Abcam), were followed by incubations with the appropriate secondary antibodies conjugated with horseradish peroxidase (HRP) and by detection using enhanced luminescence (GE Healthcare).

    Concentration Assay:

    Article Title: FOXM1: A potential indicator to predict lymphatic metastatic recurrence in stage IIA esophageal squamous cell carcinoma
    Article Snippet: Protein was extracted from tissue samples, and the concentration was determined using a bicinchoninic acid kit (Thermo Fisher Scientific, Waltham, MA, USA). .. Membranes were incubated overnight at 4°C with primary antibodies anti‐FOXM1 (1:500) and anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) (1: 5000; Abcam, Cambridge, MA, USA).

    Lysis:

    Article Title: The Role of Monocarboxylate Transporters and Their Chaperone CD147 in Lactate Efflux Inhibition and the Anticancer Effects of Terminalia chebula in Neuroblastoma Cell Line N2-A
    Article Snippet: Primary antibodies monocarboxylate transporter 1(MCT1), monocarboxylate transporter 3 (MCT3), monocarboxylate transporter 4 (MCT4), Basigin (CD147), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), secondary antibody and chemiluminescence reagent, were provided by Abcam (Cambridge, MA, USA). .. RIPA lysis buffer and mammalian protease arrest were obtained from G-Biosciences (St. Louis, MO, USA).

    Article Title: Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model
    Article Snippet: Western blot analysis The cartilages of rats were crushed in ice-cold lysis buffer (1 M Tris-HCl, pH 7.5, with protease inhibitors; 25 mM NaF; 10 mM NaV; 0.5 M EDTA; and 1% Triton X-100) and centrifuged at 14,000 rpm for 20 min. .. The membranes were blocked with 5% milk in Tris-buffered saline and Tween-20 buffer (10 mM Tris, 100 mM NaCl, and 0.1% Tween-20, pH 8.0) and subsequently probed with primary antibodies against heat-shock protein 70 (HSP-70), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), IL-1β, NF-κB, TNF-α, MMP3/7/9 (1:1,000; Abcam, Cambridge, MA, USA), inducible nitric oxide synthase (iNOS, 1:1,000 dilution, BD Transduction Laboratory, San Diego, CA, USA), p-Erk1/2, COX-2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) overnight at 4℃.

    Article Title: Human fetal skin-derived stem cell secretome enhances radiation-induced skin injury therapeutic effects by promoting angiogenesis
    Article Snippet: Western blot Proteins were extracted from the healed skin tissue in the lysis buffer. .. Resolved proteins were then electro blotted onto nitrocellulose membranes and probed with antibody against Col1A2, Col3A1, TGF-β1, TGF-β3, Ang-1, Ang-2, VEGF, PLGF, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000 dilution, Abcam, Cambridge, UK) overnight at 4 °C.

    Article Title: Iron overload induced death of osteoblasts in vitro: involvement of the mitochondrial apoptotic pathway
    Article Snippet: Primary antibodies against cytochrome c, bcl-2, bax, cleaved Caspase-3, Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and beta-actin (β -actin) were purchased from Abcam (Cambridge, UK). .. Cell Mitochondria Isolation Kit, Enhanced Chemiluminescence detection kit, Western and immunoprecipitation (IP) Cell Lysis Kit, Bicinchoninic Acid Protein (BCA) Protein Assay Kit, and 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolcarbocyanineiodide (JC-1) were purchased from Beyotime (China).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Abcam glyceraldehyde 3 phosphate dehydrogenase gapdh
    Effect of hypoxia and hyperglycemia on nuclear AGTR2 in OSCC cells ( A ) AGTR2 protein levels in nuclear and membrane fractions from OSCC cells. <t>Glyceraldehyde-3-phosphate</t> dehydrogenase <t>(GAPDH)</t> was examined as a cytoplastic marker. ( B ) Effect of hypoxia (1%) and hyperglycemia (450 mg/dl) on the nuclear localization of AGTR2 in OSCC cells. ( C–E ) Effect of hypoxia and hyperglycemia on 5-FU-induced apoptosis (C), oxidative stress (4-HNE; D) and redox status (GSH/GSSH ratio; E) in OSCC cells. Error bar, S.D. Nuclear ext, nuclear fracture; Memb ext, membrane fracture.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Abcam
    Average 90 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Abcam anti bax
    Analysis of protein expression in Hepa1c1c7 cells. The expression of proteins was quantified by flow cytometry after 24 h of treatment with PHO-S, DODAC and DODAC/PHO-S. The bar graphs show the level of expression of TRAIL - DR4 receptor ( a ); protein caspase 3 ( b ); caspase-8 protein ( c ); Free cytochrome c ( d ); protein <t>Bax</t> ( e ); protein <t>Bcl-2</t> ( f ). Values are expressed as a mean ± SD standard deviation of three independent experiments. Level of significance * P
    Anti Bax, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax/product/Abcam
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    anti bax - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Effect of hypoxia and hyperglycemia on nuclear AGTR2 in OSCC cells ( A ) AGTR2 protein levels in nuclear and membrane fractions from OSCC cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was examined as a cytoplastic marker. ( B ) Effect of hypoxia (1%) and hyperglycemia (450 mg/dl) on the nuclear localization of AGTR2 in OSCC cells. ( C–E ) Effect of hypoxia and hyperglycemia on 5-FU-induced apoptosis (C), oxidative stress (4-HNE; D) and redox status (GSH/GSSH ratio; E) in OSCC cells. Error bar, S.D. Nuclear ext, nuclear fracture; Memb ext, membrane fracture.

    Journal: Oncotarget

    Article Title: Significance of intranuclear angiotensin-II type 2 receptor in oral squamous cell carcinoma

    doi: 10.18632/oncotarget.26337

    Figure Lengend Snippet: Effect of hypoxia and hyperglycemia on nuclear AGTR2 in OSCC cells ( A ) AGTR2 protein levels in nuclear and membrane fractions from OSCC cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was examined as a cytoplastic marker. ( B ) Effect of hypoxia (1%) and hyperglycemia (450 mg/dl) on the nuclear localization of AGTR2 in OSCC cells. ( C–E ) Effect of hypoxia and hyperglycemia on 5-FU-induced apoptosis (C), oxidative stress (4-HNE; D) and redox status (GSH/GSSH ratio; E) in OSCC cells. Error bar, S.D. Nuclear ext, nuclear fracture; Memb ext, membrane fracture.

    Article Snippet: The following antibodies were used: A-II (Serotec Ltd., Oxford, UK), AGT (R & D Systems Inc., Minneapolis, MN, USA), AGTR1 (Assay Designs, Inc, Ann Arbor, MI, USA), AGTR2 (Assay Designs), renin (AnaSpec, Inc., San Jose, CA, USA), angiotensin I-converting enzyme (ACE; Life Span Biosciences, Inc., Salt Lake City, UT, USA), cathepsin G (Santa-Cruz Biotechnology, Inc., Santa Cruz, CA, USA), extracellular signal–regulated kinase (ERK)1/2, phosphorylated ERK1/2 (pERK1/2, Santa Cruz), p38 (Santa Cruz), phosphorylated p38 (pp38, Santa Cruz), importin β1/KPNB1, expotin 1/CRM1, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam), and B-cell lymphoma 2 (Bcl-2; DAKO Corp, Carpinteria, CA, USA).

    Techniques: Marker

    Analysis of protein expression in Hepa1c1c7 cells. The expression of proteins was quantified by flow cytometry after 24 h of treatment with PHO-S, DODAC and DODAC/PHO-S. The bar graphs show the level of expression of TRAIL - DR4 receptor ( a ); protein caspase 3 ( b ); caspase-8 protein ( c ); Free cytochrome c ( d ); protein Bax ( e ); protein Bcl-2 ( f ). Values are expressed as a mean ± SD standard deviation of three independent experiments. Level of significance * P

    Journal: BMC Pharmacology & Toxicology

    Article Title: Antiproliferative and proapoptotic effects of DODAC/synthetic phosphoethanolamine on hepatocellular carcinoma cells

    doi: 10.1186/s40360-018-0225-2

    Figure Lengend Snippet: Analysis of protein expression in Hepa1c1c7 cells. The expression of proteins was quantified by flow cytometry after 24 h of treatment with PHO-S, DODAC and DODAC/PHO-S. The bar graphs show the level of expression of TRAIL - DR4 receptor ( a ); protein caspase 3 ( b ); caspase-8 protein ( c ); Free cytochrome c ( d ); protein Bax ( e ); protein Bcl-2 ( f ). Values are expressed as a mean ± SD standard deviation of three independent experiments. Level of significance * P

    Article Snippet: In brief, the Hepa1c1c7 cells (1 × 105 cells/well, the cell density reached 80 to 90% confluence), were treated with PHO-S (0.3–2.0 mM), DODAC/PHO-S 1:1 (0.3–2.0 mM), and empty DODAC (0.3–2.0 mM), for 12 h, were washed with PBS and resuspended in FACS buffer with 2.5% paraformaldehyde for 1 h. After washing, cells were again resuspended in a primary antibody specific for the proteins anti-CD44, anti-CD90, anti-p53, anti-p21, anti-p27, anti-Bax, anti-Bcl-2, anti-caspase-3, anti-caspase-8 (Abcam, Cambridge, MA, United States); anti- cytochrome c, anti-DR4 (Santa (Cruz Biotechnology Inc., Santa Cruz, EUA) and anti-cyclin D1 (Cell Signaling Technology, Danvers, MA), at a concentration of 1 μg/ml at 4 °C, for 1 min.

    Techniques: Expressing, Flow Cytometry, Cytometry, Standard Deviation

    SR induces apoptosis in DMBA-exposed cells in vitro . (A) Flow cytometric analysis was carried out to calculate the apoptotic cells under various treatments. (B) The quantification of apoptotic cells is shown following flow cytometry. (C) Western blot analysis was performed to evaluate p21, PUMA, Bax and cleaved caspase-3 protein levels in cells with DMBA induction accompanied by or without SR administration. Data are presented as mean ± SEM (n=20). + P

    Journal: Oncology Reports

    Article Title: Salidroside prevents skin carcinogenesis induced by DMBA/TPA in a mouse model through suppression of inflammation and promotion of apoptosis

    doi: 10.3892/or.2018.6381

    Figure Lengend Snippet: SR induces apoptosis in DMBA-exposed cells in vitro . (A) Flow cytometric analysis was carried out to calculate the apoptotic cells under various treatments. (B) The quantification of apoptotic cells is shown following flow cytometry. (C) Western blot analysis was performed to evaluate p21, PUMA, Bax and cleaved caspase-3 protein levels in cells with DMBA induction accompanied by or without SR administration. Data are presented as mean ± SEM (n=20). + P

    Article Snippet: The primary antibodies used in our study included: anti-p21 (1:1,000, ab86696), anti-PUMA (1:1,000, ab9643), anti-Bax (1:1,000, ab32503), anti-p53 (1:1,000, ab131442), anti-caspase-3 (1:1,000, ab90437), anti-IκBα (1:1,000, ab32518), anti-p-IκBα (1:1,000, ab133462), anti-NF-κB (1:1,000, ab16502), anti-p-NF-κB (1:1,000, ab86299) and GAPDH (1:1,000, ab8245) all from Abcam.

    Techniques: In Vitro, Flow Cytometry, Cytometry, Western Blot

    SR promotes apoptosis following DMBA/TPA treatment in mice. (A) The representative images of p21, PUMA, Bax and cleaved caspase-3 protein bands. (B) The quantification of p21, PUMA, Bax and cleaved caspase-3 is shown. Data are presented as mean ± SEM (n=20). + P

    Journal: Oncology Reports

    Article Title: Salidroside prevents skin carcinogenesis induced by DMBA/TPA in a mouse model through suppression of inflammation and promotion of apoptosis

    doi: 10.3892/or.2018.6381

    Figure Lengend Snippet: SR promotes apoptosis following DMBA/TPA treatment in mice. (A) The representative images of p21, PUMA, Bax and cleaved caspase-3 protein bands. (B) The quantification of p21, PUMA, Bax and cleaved caspase-3 is shown. Data are presented as mean ± SEM (n=20). + P

    Article Snippet: The primary antibodies used in our study included: anti-p21 (1:1,000, ab86696), anti-PUMA (1:1,000, ab9643), anti-Bax (1:1,000, ab32503), anti-p53 (1:1,000, ab131442), anti-caspase-3 (1:1,000, ab90437), anti-IκBα (1:1,000, ab32518), anti-p-IκBα (1:1,000, ab133462), anti-NF-κB (1:1,000, ab16502), anti-p-NF-κB (1:1,000, ab86299) and GAPDH (1:1,000, ab8245) all from Abcam.

    Techniques: Mouse Assay

    Ad-DC315-mE1A or Ad-DC315-E1A induced inactive p53 expression HCT116 p53+/+, HCT116 p53−/−, H1299, Hela, HepG2 and U2OS cells were infected with Ad-DC315, Ad-DC315-E1A, Ad-DC315-mE1A or Ad-DC315-EGFP at MOI=20 for 48h. The level of p53, p21, mdm2, Bax, Bcl-2, PARP and E1A proteins were analysed by western blotting. Beta-actin was assayed as a loading control.

    Journal: Oncotarget

    Article Title: A p53-independent apoptotic mechanism of adenoviral mutant E1A was involved in its selective antitumor activity for human cancer

    doi: 10.18632/oncotarget.10221

    Figure Lengend Snippet: Ad-DC315-mE1A or Ad-DC315-E1A induced inactive p53 expression HCT116 p53+/+, HCT116 p53−/−, H1299, Hela, HepG2 and U2OS cells were infected with Ad-DC315, Ad-DC315-E1A, Ad-DC315-mE1A or Ad-DC315-EGFP at MOI=20 for 48h. The level of p53, p21, mdm2, Bax, Bcl-2, PARP and E1A proteins were analysed by western blotting. Beta-actin was assayed as a loading control.

    Article Snippet: Primary antibodies used for detection were: anti-p53 (DO1, Santa Cruz Biotechnology, USA), anti-mdm2, anti-p21 (Santa Cruz Biotechnology, USA), anti-E1A (clone M73, Merck millipore), anti-Rb, anti-PARP, anti-Bax, anti-Bcl-2, anti-H1 and anti-beta-actin (Abcam, Cambridge, MA, USA).

    Techniques: Expressing, Infection, Western Blot