rabbit mab against glyceraldehyde 3 phosphate dehydrogenase gapdh  (Santa Cruz Biotechnology)

 
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    Santa Cruz Biotechnology rabbit mab against glyceraldehyde 3 phosphate dehydrogenase gapdh
    Rabbit Mab Against Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit mab against glyceraldehyde 3 phosphate dehydrogenase gapdh/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit mab against glyceraldehyde 3 phosphate dehydrogenase gapdh - by Bioz Stars, 2024-07
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    mouse anti rabbit glyceraldehyde 3 phosphate dehydrogenase gapdh monoclonal antibody  (Biosynth Carbosynth)


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    Biosynth Carbosynth mouse anti rabbit glyceraldehyde 3 phosphate dehydrogenase gapdh monoclonal antibody

    Mouse Anti Rabbit Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Monoclonal Antibody, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti rabbit glyceraldehyde 3 phosphate dehydrogenase gapdh monoclonal antibody/product/Biosynth Carbosynth
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti rabbit glyceraldehyde 3 phosphate dehydrogenase gapdh monoclonal antibody - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair"

    Article Title: Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.113282


    Figure Legend Snippet:

    Techniques Used: Recombinant, Plasmid Preparation, Software

    glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab  (Danaher Inc)


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    Danaher Inc glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab
    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Rabbit Mab, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway"

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    Journal: Journal of Virology

    doi: 10.1128/jvi.01982-23

    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
    Figure Legend Snippet: pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Techniques Used: Transfection, Infection, Over Expression, Quantitative RT-PCR, Expressing, Western Blot

    Knockdown or knockout of NLRP1 enhances PDCoV replication. IPI-2I cells were transfected with siNLRP1#1, siNLRP1#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of NLRP1 and PDCoV-N in NLRP1 knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis (ST) cells. (D–F) Expression of NLRP1 and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (G and I) WT IPI-2I cells and NLRP1−/− IPI-2I cells were infected with PDCoV at an MOI of 0.1 for 24 h, and then the relative mRNA levels and protein levels of PDCoV-N were detected by RT-qPCR (G) and Western blotting (I). (H) The PDCoV TCID50 in the supernatants of WT or NLRP1−/− IPI-2I cell was titrated on ST cells. (J) The relative intensity of PDCoV-N was normalized to that of GAPDH. The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: Knockdown or knockout of NLRP1 enhances PDCoV replication. IPI-2I cells were transfected with siNLRP1#1, siNLRP1#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of NLRP1 and PDCoV-N in NLRP1 knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis (ST) cells. (D–F) Expression of NLRP1 and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (G and I) WT IPI-2I cells and NLRP1−/− IPI-2I cells were infected with PDCoV at an MOI of 0.1 for 24 h, and then the relative mRNA levels and protein levels of PDCoV-N were detected by RT-qPCR (G) and Western blotting (I). (H) The PDCoV TCID50 in the supernatants of WT or NLRP1−/− IPI-2I cell was titrated on ST cells. (J) The relative intensity of PDCoV-N was normalized to that of GAPDH. The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Knock-Out, Transfection, Infection, Expressing, Western Blot, Quantitative RT-PCR

    The treatment of IL-11 recombinant protein inhibits the replication of PDCoV. After incubation with 0.1 MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with phosphate-buffered saline (PBS) to remove unbound virus and then treated with different concentrations of IL-11 protein (0, 10, 100, or 1,000 ng/mL) for 24 h. (A) The relative viral copy number of PDCoV and the protein expression level of PDCoV-N were measured by RT-qPCR and Western blotting, respectively. (B) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) The PDCoV TCID50 in the supernatants of IPI-2I cells was titrated on swine testis cells. (D–F) The relative viral RNA copy number of PDCoV in IL-11-treated cells. After incubation with 0.01 (F), 0.1 (E), or 1 (D) MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with PBS to remove unbound virus and then treated with 100 ng/mL of IL-11 protein for 24 h. The means and SD of the results from three independent experiments are shown. ns, no significant difference. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.
    Figure Legend Snippet: The treatment of IL-11 recombinant protein inhibits the replication of PDCoV. After incubation with 0.1 MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with phosphate-buffered saline (PBS) to remove unbound virus and then treated with different concentrations of IL-11 protein (0, 10, 100, or 1,000 ng/mL) for 24 h. (A) The relative viral copy number of PDCoV and the protein expression level of PDCoV-N were measured by RT-qPCR and Western blotting, respectively. (B) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) The PDCoV TCID50 in the supernatants of IPI-2I cells was titrated on swine testis cells. (D–F) The relative viral RNA copy number of PDCoV in IL-11-treated cells. After incubation with 0.01 (F), 0.1 (E), or 1 (D) MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with PBS to remove unbound virus and then treated with 100 ng/mL of IL-11 protein for 24 h. The means and SD of the results from three independent experiments are shown. ns, no significant difference. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Techniques Used: Recombinant, Incubation, Saline, Virus, Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of IL-11RA enhances PDCoV infection. IPI-2I cells were transfected with IL-11RA#1, IL-11RA#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of IL-11RA and PDCoV-N in IL-11RA knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D–F) Expression of IL-11RA and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: Knockdown of IL-11RA enhances PDCoV infection. IPI-2I cells were transfected with IL-11RA#1, IL-11RA#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of IL-11RA and PDCoV-N in IL-11RA knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D–F) Expression of IL-11RA and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Infection, Transfection, Expressing, Western Blot

    NLRP1 relies on the IL-11 pathway to inhibit PDCoV replication. After transfection with 50 nM of siIL-11RA#2 or NC for 12 h, IPI-2I cells were transfected with 1 µg of Flag-tagged NLRP1 for 24 h and then infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) The relative expression of NLRP1 and PDCoV-N in NC (A) or IL-11-knockdown cells (B). (C) The protein expression of PDCoV-N was detected by Western blotting. (D) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ns, no significant difference. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: NLRP1 relies on the IL-11 pathway to inhibit PDCoV replication. After transfection with 50 nM of siIL-11RA#2 or NC for 12 h, IPI-2I cells were transfected with 1 µg of Flag-tagged NLRP1 for 24 h and then infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) The relative expression of NLRP1 and PDCoV-N in NC (A) or IL-11-knockdown cells (B). (C) The protein expression of PDCoV-N was detected by Western blotting. (D) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ns, no significant difference. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Transfection, Infection, Expressing, Western Blot

    The treatment of IL-11 inhibits the phosphorylation of the ERK signaling pathway. After infection with PDCoV at an MOI of 1, IPI-2I cells were treated with different doses (0, 10, 100, and 500) μg/mL of recombinant IL-11 protein. (A) The total proteins and phosphorylation proteins’ expression of ERK1, ERK2, AKT1, and STAT3 were measured by Western blotting. (B–F) The relative intensities of PDCoV-N (B), pERK1 (C), pERK2 (D), pAKT1 (E), and pSTAT3 (F) were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: The treatment of IL-11 inhibits the phosphorylation of the ERK signaling pathway. After infection with PDCoV at an MOI of 1, IPI-2I cells were treated with different doses (0, 10, 100, and 500) μg/mL of recombinant IL-11 protein. (A) The total proteins and phosphorylation proteins’ expression of ERK1, ERK2, AKT1, and STAT3 were measured by Western blotting. (B–F) The relative intensities of PDCoV-N (B), pERK1 (C), pERK2 (D), pAKT1 (E), and pSTAT3 (F) were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Infection, Recombinant, Expressing, Western Blot

    glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
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    Danaher Inc glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab
    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Rabbit Mab, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway"

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    Journal: Journal of Virology

    doi: 10.1128/jvi.01982-23

    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
    Figure Legend Snippet: pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Techniques Used: Transfection, Infection, Over Expression, Quantitative RT-PCR, Expressing, Western Blot

    Knockdown or knockout of NLRP1 enhances PDCoV replication. IPI-2I cells were transfected with siNLRP1#1, siNLRP1#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of NLRP1 and PDCoV-N in NLRP1 knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis (ST) cells. (D–F) Expression of NLRP1 and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (G and I) WT IPI-2I cells and NLRP1−/− IPI-2I cells were infected with PDCoV at an MOI of 0.1 for 24 h, and then the relative mRNA levels and protein levels of PDCoV-N were detected by RT-qPCR (G) and Western blotting (I). (H) The PDCoV TCID50 in the supernatants of WT or NLRP1−/− IPI-2I cell was titrated on ST cells. (J) The relative intensity of PDCoV-N was normalized to that of GAPDH. The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: Knockdown or knockout of NLRP1 enhances PDCoV replication. IPI-2I cells were transfected with siNLRP1#1, siNLRP1#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of NLRP1 and PDCoV-N in NLRP1 knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis (ST) cells. (D–F) Expression of NLRP1 and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (G and I) WT IPI-2I cells and NLRP1−/− IPI-2I cells were infected with PDCoV at an MOI of 0.1 for 24 h, and then the relative mRNA levels and protein levels of PDCoV-N were detected by RT-qPCR (G) and Western blotting (I). (H) The PDCoV TCID50 in the supernatants of WT or NLRP1−/− IPI-2I cell was titrated on ST cells. (J) The relative intensity of PDCoV-N was normalized to that of GAPDH. The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Knock-Out, Transfection, Infection, Expressing, Western Blot, Quantitative RT-PCR

    The treatment of IL-11 recombinant protein inhibits the replication of PDCoV. After incubation with 0.1 MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with phosphate-buffered saline (PBS) to remove unbound virus and then treated with different concentrations of IL-11 protein (0, 10, 100, or 1,000 ng/mL) for 24 h. (A) The relative viral copy number of PDCoV and the protein expression level of PDCoV-N were measured by RT-qPCR and Western blotting, respectively. (B) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) The PDCoV TCID50 in the supernatants of IPI-2I cells was titrated on swine testis cells. (D–F) The relative viral RNA copy number of PDCoV in IL-11-treated cells. After incubation with 0.01 (F), 0.1 (E), or 1 (D) MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with PBS to remove unbound virus and then treated with 100 ng/mL of IL-11 protein for 24 h. The means and SD of the results from three independent experiments are shown. ns, no significant difference. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.
    Figure Legend Snippet: The treatment of IL-11 recombinant protein inhibits the replication of PDCoV. After incubation with 0.1 MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with phosphate-buffered saline (PBS) to remove unbound virus and then treated with different concentrations of IL-11 protein (0, 10, 100, or 1,000 ng/mL) for 24 h. (A) The relative viral copy number of PDCoV and the protein expression level of PDCoV-N were measured by RT-qPCR and Western blotting, respectively. (B) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) The PDCoV TCID50 in the supernatants of IPI-2I cells was titrated on swine testis cells. (D–F) The relative viral RNA copy number of PDCoV in IL-11-treated cells. After incubation with 0.01 (F), 0.1 (E), or 1 (D) MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with PBS to remove unbound virus and then treated with 100 ng/mL of IL-11 protein for 24 h. The means and SD of the results from three independent experiments are shown. ns, no significant difference. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Techniques Used: Recombinant, Incubation, Saline, Virus, Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of IL-11RA enhances PDCoV infection. IPI-2I cells were transfected with IL-11RA#1, IL-11RA#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of IL-11RA and PDCoV-N in IL-11RA knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D–F) Expression of IL-11RA and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: Knockdown of IL-11RA enhances PDCoV infection. IPI-2I cells were transfected with IL-11RA#1, IL-11RA#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of IL-11RA and PDCoV-N in IL-11RA knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D–F) Expression of IL-11RA and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Infection, Transfection, Expressing, Western Blot

    NLRP1 relies on the IL-11 pathway to inhibit PDCoV replication. After transfection with 50 nM of siIL-11RA#2 or NC for 12 h, IPI-2I cells were transfected with 1 µg of Flag-tagged NLRP1 for 24 h and then infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) The relative expression of NLRP1 and PDCoV-N in NC (A) or IL-11-knockdown cells (B). (C) The protein expression of PDCoV-N was detected by Western blotting. (D) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ns, no significant difference. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: NLRP1 relies on the IL-11 pathway to inhibit PDCoV replication. After transfection with 50 nM of siIL-11RA#2 or NC for 12 h, IPI-2I cells were transfected with 1 µg of Flag-tagged NLRP1 for 24 h and then infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) The relative expression of NLRP1 and PDCoV-N in NC (A) or IL-11-knockdown cells (B). (C) The protein expression of PDCoV-N was detected by Western blotting. (D) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ns, no significant difference. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Transfection, Infection, Expressing, Western Blot

    The treatment of IL-11 inhibits the phosphorylation of the ERK signaling pathway. After infection with PDCoV at an MOI of 1, IPI-2I cells were treated with different doses (0, 10, 100, and 500) μg/mL of recombinant IL-11 protein. (A) The total proteins and phosphorylation proteins’ expression of ERK1, ERK2, AKT1, and STAT3 were measured by Western blotting. (B–F) The relative intensities of PDCoV-N (B), pERK1 (C), pERK2 (D), pAKT1 (E), and pSTAT3 (F) were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.
    Figure Legend Snippet: The treatment of IL-11 inhibits the phosphorylation of the ERK signaling pathway. After infection with PDCoV at an MOI of 1, IPI-2I cells were treated with different doses (0, 10, 100, and 500) μg/mL of recombinant IL-11 protein. (A) The total proteins and phosphorylation proteins’ expression of ERK1, ERK2, AKT1, and STAT3 were measured by Western blotting. (B–F) The relative intensities of PDCoV-N (B), pERK1 (C), pERK2 (D), pAKT1 (E), and pSTAT3 (F) were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Techniques Used: Infection, Recombinant, Expressing, Western Blot

    glyceraldehyde 3 phosphate dehydrogenase gapdh d16h11 xp rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc glyceraldehyde 3 phosphate dehydrogenase gapdh d16h11 xp rabbit mab
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    rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh monoclonal antibody  (Danaher Inc)


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    Danaher Inc rabbit anti glyceraldehyde 3 phosphate dehydrogenase gapdh monoclonal antibody
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    rabbit monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibodies antibodies control  (Cell Signaling Technology Inc)


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    glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc glyceraldehyde 3 phosphate dehydrogenase gapdh rabbit mab
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    primary rabbit monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody conjugated to horseradish peroxidase  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary rabbit monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody conjugated to horseradish peroxidase
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    rabbit monoclonal α glyceraldehyde 3 phosphate dehydrogenase gapdh  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal α glyceraldehyde 3 phosphate dehydrogenase gapdh
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    Santa Cruz Biotechnology rabbit mab against glyceraldehyde 3 phosphate dehydrogenase gapdh
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    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
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    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
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    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to <t>glyceraldehyde-3-phosphate</t> dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.
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    Image Search Results


    Journal: Cell reports

    Article Title: Mitf is a Schwann cell sensor of axonal integrity that drives nerve repair

    doi: 10.1016/j.celrep.2023.113282

    Figure Lengend Snippet:

    Article Snippet: Mouse Anti-Rabbit Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Monoclonal Antibody; Clone 6C5 , Fitzgerald Industries International , Cat# 10R-G109a; RRID:AB_1285808.

    Techniques: Recombinant, Plasmid Preparation, Software

    pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Journal: Journal of Virology

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    doi: 10.1128/jvi.01982-23

    Figure Lengend Snippet: pNLRP1 inhibits the replication of PDCoV. IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PDCoV at an MOI of 0.1 for 24 h. (A) The overexpression of NLRP1 was verified by RT-qPCR. (B) The relative expression of PDCoV was determined by RT-qPCR. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D) The protein expression of NLRP1 and PDCoV-N was detected by Western blotting. (E) The relative intensities of PDCoV-N were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (F) IPI-2I cells were transfected with Flag-tagged NLRP1 (0 or 1 µg) for 24 h, followed by infection with PEDV at an MOI of 1 for 24 h. The relative expression of PEDV was determined by RT-qPCR. (G) The PEDV TCID50 in the supernatants was titrated on Vero-E6 cells. The means and SD of the results from three independent experiments are shown. **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Article Snippet: Rabbit polyclonal antibody (pAb) to AKT1, phospho-ERK1/2 rabbit monoclonal antibody (mAb), STAT3 rabbit mAb, phospho-STAT3-S727 rabbit mAb, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Abcam (Cambridge, UK).

    Techniques: Transfection, Infection, Over Expression, Quantitative RT-PCR, Expressing, Western Blot

    Knockdown or knockout of NLRP1 enhances PDCoV replication. IPI-2I cells were transfected with siNLRP1#1, siNLRP1#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of NLRP1 and PDCoV-N in NLRP1 knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis (ST) cells. (D–F) Expression of NLRP1 and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (G and I) WT IPI-2I cells and NLRP1−/− IPI-2I cells were infected with PDCoV at an MOI of 0.1 for 24 h, and then the relative mRNA levels and protein levels of PDCoV-N were detected by RT-qPCR (G) and Western blotting (I). (H) The PDCoV TCID50 in the supernatants of WT or NLRP1−/− IPI-2I cell was titrated on ST cells. (J) The relative intensity of PDCoV-N was normalized to that of GAPDH. The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Journal: Journal of Virology

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    doi: 10.1128/jvi.01982-23

    Figure Lengend Snippet: Knockdown or knockout of NLRP1 enhances PDCoV replication. IPI-2I cells were transfected with siNLRP1#1, siNLRP1#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of NLRP1 and PDCoV-N in NLRP1 knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis (ST) cells. (D–F) Expression of NLRP1 and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (G and I) WT IPI-2I cells and NLRP1−/− IPI-2I cells were infected with PDCoV at an MOI of 0.1 for 24 h, and then the relative mRNA levels and protein levels of PDCoV-N were detected by RT-qPCR (G) and Western blotting (I). (H) The PDCoV TCID50 in the supernatants of WT or NLRP1−/− IPI-2I cell was titrated on ST cells. (J) The relative intensity of PDCoV-N was normalized to that of GAPDH. The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Rabbit polyclonal antibody (pAb) to AKT1, phospho-ERK1/2 rabbit monoclonal antibody (mAb), STAT3 rabbit mAb, phospho-STAT3-S727 rabbit mAb, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Abcam (Cambridge, UK).

    Techniques: Knock-Out, Transfection, Infection, Expressing, Western Blot, Quantitative RT-PCR

    The treatment of IL-11 recombinant protein inhibits the replication of PDCoV. After incubation with 0.1 MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with phosphate-buffered saline (PBS) to remove unbound virus and then treated with different concentrations of IL-11 protein (0, 10, 100, or 1,000 ng/mL) for 24 h. (A) The relative viral copy number of PDCoV and the protein expression level of PDCoV-N were measured by RT-qPCR and Western blotting, respectively. (B) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) The PDCoV TCID50 in the supernatants of IPI-2I cells was titrated on swine testis cells. (D–F) The relative viral RNA copy number of PDCoV in IL-11-treated cells. After incubation with 0.01 (F), 0.1 (E), or 1 (D) MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with PBS to remove unbound virus and then treated with 100 ng/mL of IL-11 protein for 24 h. The means and SD of the results from three independent experiments are shown. ns, no significant difference. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Journal: Journal of Virology

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    doi: 10.1128/jvi.01982-23

    Figure Lengend Snippet: The treatment of IL-11 recombinant protein inhibits the replication of PDCoV. After incubation with 0.1 MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with phosphate-buffered saline (PBS) to remove unbound virus and then treated with different concentrations of IL-11 protein (0, 10, 100, or 1,000 ng/mL) for 24 h. (A) The relative viral copy number of PDCoV and the protein expression level of PDCoV-N were measured by RT-qPCR and Western blotting, respectively. (B) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) The PDCoV TCID50 in the supernatants of IPI-2I cells was titrated on swine testis cells. (D–F) The relative viral RNA copy number of PDCoV in IL-11-treated cells. After incubation with 0.01 (F), 0.1 (E), or 1 (D) MOI of PDCoV for 2 h at 37°C, IPI-2I cells were washed three times with PBS to remove unbound virus and then treated with 100 ng/mL of IL-11 protein for 24 h. The means and SD of the results from three independent experiments are shown. ns, no significant difference. *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.

    Article Snippet: Rabbit polyclonal antibody (pAb) to AKT1, phospho-ERK1/2 rabbit monoclonal antibody (mAb), STAT3 rabbit mAb, phospho-STAT3-S727 rabbit mAb, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Abcam (Cambridge, UK).

    Techniques: Recombinant, Incubation, Saline, Virus, Expressing, Quantitative RT-PCR, Western Blot

    Knockdown of IL-11RA enhances PDCoV infection. IPI-2I cells were transfected with IL-11RA#1, IL-11RA#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of IL-11RA and PDCoV-N in IL-11RA knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D–F) Expression of IL-11RA and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Journal: Journal of Virology

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    doi: 10.1128/jvi.01982-23

    Figure Lengend Snippet: Knockdown of IL-11RA enhances PDCoV infection. IPI-2I cells were transfected with IL-11RA#1, IL-11RA#2, or NC at 50 nM for 24 h and subsequently infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) mRNA levels of IL-11RA and PDCoV-N in IL-11RA knockdown cells. (C) The PDCoV TCID50 in the supernatants was titrated on swine testis cells. (D–F) Expression of IL-11RA and PDCoV-N was detected by Western blotting, and the relative intensities of NLRP1 and PDCoV-N were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Rabbit polyclonal antibody (pAb) to AKT1, phospho-ERK1/2 rabbit monoclonal antibody (mAb), STAT3 rabbit mAb, phospho-STAT3-S727 rabbit mAb, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Abcam (Cambridge, UK).

    Techniques: Infection, Transfection, Expressing, Western Blot

    NLRP1 relies on the IL-11 pathway to inhibit PDCoV replication. After transfection with 50 nM of siIL-11RA#2 or NC for 12 h, IPI-2I cells were transfected with 1 µg of Flag-tagged NLRP1 for 24 h and then infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) The relative expression of NLRP1 and PDCoV-N in NC (A) or IL-11-knockdown cells (B). (C) The protein expression of PDCoV-N was detected by Western blotting. (D) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ns, no significant difference. ***P < 0.001 and ****P < 0.0001.

    Journal: Journal of Virology

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    doi: 10.1128/jvi.01982-23

    Figure Lengend Snippet: NLRP1 relies on the IL-11 pathway to inhibit PDCoV replication. After transfection with 50 nM of siIL-11RA#2 or NC for 12 h, IPI-2I cells were transfected with 1 µg of Flag-tagged NLRP1 for 24 h and then infected with PDCoV at an MOI of 0.1 for 24 h. (A and B) The relative expression of NLRP1 and PDCoV-N in NC (A) or IL-11-knockdown cells (B). (C) The protein expression of PDCoV-N was detected by Western blotting. (D) The relative intensity of PDCoV-N was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ns, no significant difference. ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Rabbit polyclonal antibody (pAb) to AKT1, phospho-ERK1/2 rabbit monoclonal antibody (mAb), STAT3 rabbit mAb, phospho-STAT3-S727 rabbit mAb, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Abcam (Cambridge, UK).

    Techniques: Transfection, Infection, Expressing, Western Blot

    The treatment of IL-11 inhibits the phosphorylation of the ERK signaling pathway. After infection with PDCoV at an MOI of 1, IPI-2I cells were treated with different doses (0, 10, 100, and 500) μg/mL of recombinant IL-11 protein. (A) The total proteins and phosphorylation proteins’ expression of ERK1, ERK2, AKT1, and STAT3 were measured by Western blotting. (B–F) The relative intensities of PDCoV-N (B), pERK1 (C), pERK2 (D), pAKT1 (E), and pSTAT3 (F) were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Journal: Journal of Virology

    Article Title: NLRP1 restricts porcine deltacoronavirus infection via IL-11 inhibiting the phosphorylation of the ERK signaling pathway

    doi: 10.1128/jvi.01982-23

    Figure Lengend Snippet: The treatment of IL-11 inhibits the phosphorylation of the ERK signaling pathway. After infection with PDCoV at an MOI of 1, IPI-2I cells were treated with different doses (0, 10, 100, and 500) μg/mL of recombinant IL-11 protein. (A) The total proteins and phosphorylation proteins’ expression of ERK1, ERK2, AKT1, and STAT3 were measured by Western blotting. (B–F) The relative intensities of PDCoV-N (B), pERK1 (C), pERK2 (D), pAKT1 (E), and pSTAT3 (F) were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The means and SD of the results from three independent experiments are shown. ***P < 0.001 and ****P < 0.0001.

    Article Snippet: Rabbit polyclonal antibody (pAb) to AKT1, phospho-ERK1/2 rabbit monoclonal antibody (mAb), STAT3 rabbit mAb, phospho-STAT3-S727 rabbit mAb, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) rabbit mAb were purchased from Abcam (Cambridge, UK).

    Techniques: Infection, Recombinant, Expressing, Western Blot