glyceraldehyde 3 phosphate dehydrogenase gapdh abs16  (Merck KGaA)

 
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    Structured Review

    Merck KGaA glyceraldehyde 3 phosphate dehydrogenase gapdh abs16
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Abs16, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh abs16/product/Merck KGaA
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh abs16 - by Bioz Stars, 2020-04
    95/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: An equal amount of denatured protein samples (40 μ g) was loaded per well for 12% SDS-polyacrylamide gel electrophoresis (Bio-Rad, USA) and transferred to PVDF membranes. .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Multiple Displacement Amplification:

    Article Title: Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway
    Article Snippet: Western blot analysis The MCF-7 or MDA-MB-231 (2×105 cells) were seeded into 6-well plates and exposed vaqrious concentrations of GFPs for the indicated periods of time. .. The membranes were incubated at 4°C overnight with Bcl-2 (MABC573), Bcl-extra large (Bcl-xL; MAB4625), Bax (AB2915), cleaved caspase-3 (AB3623), cleaved caspase-8 (AB1879), and phosphorylated (p)-AKT (05–1003) (all from Merck Millipore, Darmstadt, Germany), total (t)-AKT (ab126811) and p-glycogen synthase kinase-3β (GSK-3β) (ab75745) (both from Abcam, Cambrige, UK), T-GSK-3β (PK1111) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (both from Merck Millipore) at dilution of 1:1,000.

    Protease Inhibitor:

    Article Title: Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway
    Article Snippet: The cells and collected tumor tissues were lysed by RIPA buffer containing 1% protease inhibitor cocktail and 2% phenylmethanesulfonyl fluoride (PMSF) (both from Sigma-Aldrich). .. The membranes were incubated at 4°C overnight with Bcl-2 (MABC573), Bcl-extra large (Bcl-xL; MAB4625), Bax (AB2915), cleaved caspase-3 (AB3623), cleaved caspase-8 (AB1879), and phosphorylated (p)-AKT (05–1003) (all from Merck Millipore, Darmstadt, Germany), total (t)-AKT (ab126811) and p-glycogen synthase kinase-3β (GSK-3β) (ab75745) (both from Abcam, Cambrige, UK), T-GSK-3β (PK1111) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (both from Merck Millipore) at dilution of 1:1,000.

    Western Blot:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: Paragraph title: 2.6. Western Blot Analysis ... Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Article Title: Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway
    Article Snippet: Paragraph title: Western blot analysis ... The membranes were incubated at 4°C overnight with Bcl-2 (MABC573), Bcl-extra large (Bcl-xL; MAB4625), Bax (AB2915), cleaved caspase-3 (AB3623), cleaved caspase-8 (AB1879), and phosphorylated (p)-AKT (05–1003) (all from Merck Millipore, Darmstadt, Germany), total (t)-AKT (ab126811) and p-glycogen synthase kinase-3β (GSK-3β) (ab75745) (both from Abcam, Cambrige, UK), T-GSK-3β (PK1111) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (both from Merck Millipore) at dilution of 1:1,000.

    Mouse Assay:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: One part of liver tissues obtained from CFS mice was extracted with lysis buffer (RIPA with protease and phosphatase inhibitor) for 30 min on ice and then centrifuged at 10000 rpm for 10 min at 4°C to remove the precipitate. .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Concentration Assay:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: The membranes were blocked using 5% bovine serum albumin (BSA) at room temperature for 2 h. The blots were incubated with the appropriate concentration of specific antibody overnight at 4°C. .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Incubation:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: The membranes were blocked using 5% bovine serum albumin (BSA) at room temperature for 2 h. The blots were incubated with the appropriate concentration of specific antibody overnight at 4°C. .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Article Title: Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway
    Article Snippet: .. The membranes were incubated at 4°C overnight with Bcl-2 (MABC573), Bcl-extra large (Bcl-xL; MAB4625), Bax (AB2915), cleaved caspase-3 (AB3623), cleaved caspase-8 (AB1879), and phosphorylated (p)-AKT (05–1003) (all from Merck Millipore, Darmstadt, Germany), total (t)-AKT (ab126811) and p-glycogen synthase kinase-3β (GSK-3β) (ab75745) (both from Abcam, Cambrige, UK), T-GSK-3β (PK1111) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (both from Merck Millipore) at dilution of 1:1,000. .. The membranes were then incubated with horseradish peroxidase-conjugated secondary antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) for 2 h at room temperature.

    Expressing:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000. .. The quantification of protein expression was determined using the ImageJ 1.46 software (Rasband, Bethesda, MD, USA).

    BIA-KA:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: The concentration of total protein was determined by a bicinchoninic acid (BCA) protein assay kit (Merck Millipore, USA). .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Lysis:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: One part of liver tissues obtained from CFS mice was extracted with lysis buffer (RIPA with protease and phosphatase inhibitor) for 30 min on ice and then centrifuged at 10000 rpm for 10 min at 4°C to remove the precipitate. .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: .. Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000. .. The bonds were washed with TBS buffer plus 0.1% Tween-20 for five times and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (sc-3836) (Santa Cruz Biotechnology, Santa Cruz, USA) for 4 h at 4°C.

    SDS Page:

    Article Title: Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway
    Article Snippet: Protein samples (40 μ g) were separated on a 12% SDS-PAGE gel, and then electroblotted onto nitrocellulose membranes (0.45 μ m; Bio Basic, Inc., Markham, ON, Canada). .. The membranes were incubated at 4°C overnight with Bcl-2 (MABC573), Bcl-extra large (Bcl-xL; MAB4625), Bax (AB2915), cleaved caspase-3 (AB3623), cleaved caspase-8 (AB1879), and phosphorylated (p)-AKT (05–1003) (all from Merck Millipore, Darmstadt, Germany), total (t)-AKT (ab126811) and p-glycogen synthase kinase-3β (GSK-3β) (ab75745) (both from Abcam, Cambrige, UK), T-GSK-3β (PK1111) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (both from Merck Millipore) at dilution of 1:1,000.

    Software:

    Article Title: Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress
    Article Snippet: Primary antibodies Nrf2 (ab137550), SOD1 (ab16831), SOD2 (ab131443), heme oxygenase-1 (HO-1) (ab25901), catalase (CAT) (ab7970) (Abcam, Cambridge, USA), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (Merck Millipore, Darmstadt, Germany) were diluted at 1 : 2000. .. The quantification of protein expression was determined using the ImageJ 1.46 software (Rasband, Bethesda, MD, USA).

    Article Title: Grifola frondosa polysaccharides induce breast cancer cell apoptosis via the mitochondrial-dependent apoptotic pathway
    Article Snippet: The membranes were incubated at 4°C overnight with Bcl-2 (MABC573), Bcl-extra large (Bcl-xL; MAB4625), Bax (AB2915), cleaved caspase-3 (AB3623), cleaved caspase-8 (AB1879), and phosphorylated (p)-AKT (05–1003) (all from Merck Millipore, Darmstadt, Germany), total (t)-AKT (ab126811) and p-glycogen synthase kinase-3β (GSK-3β) (ab75745) (both from Abcam, Cambrige, UK), T-GSK-3β (PK1111) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (ABS16) (both from Merck Millipore) at dilution of 1:1,000. .. The intensity of the bands was quantified using ImageJ software.

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  • 94
    Merck KGaA glyceraldehyde 3 phosphate dehydrogenase
    Four weeks of SCU treatment increased the expression levels of Nrf2, HO-1, SOD1, SOD2, and CAT in the kidney of db/db mice. The data on quantified protein expressions were normalized by related <t>glyceraldehyde-3-phosphate</t> dehydrogenase. The results are represented as means ± SEM ( n = 4). # P
    Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase/product/Merck KGaA
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    95
    Merck KGaA glyceraldehyde 3 phosphate dehydrogenase gapdh abs16
    Four weeks of SCU treatment increased the expression levels of Nrf2, HO-1, SOD1, SOD2, and CAT in the kidney of db/db mice. The data on quantified protein expressions were normalized by related <t>glyceraldehyde-3-phosphate</t> dehydrogenase. The results are represented as means ± SEM ( n = 4). # P
    Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Abs16, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glyceraldehyde 3 phosphate dehydrogenase gapdh abs16/product/Merck KGaA
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    glyceraldehyde 3 phosphate dehydrogenase gapdh abs16 - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    96
    Merck KGaA reference protein glyceraldehyde 3 phosphate dehydrogenase
    Four weeks of SCU treatment increased the expression levels of Nrf2, HO-1, SOD1, SOD2, and CAT in the kidney of db/db mice. The data on quantified protein expressions were normalized by related <t>glyceraldehyde-3-phosphate</t> dehydrogenase. The results are represented as means ± SEM ( n = 4). # P
    Reference Protein Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference protein glyceraldehyde 3 phosphate dehydrogenase/product/Merck KGaA
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reference protein glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

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    Four weeks of SCU treatment increased the expression levels of Nrf2, HO-1, SOD1, SOD2, and CAT in the kidney of db/db mice. The data on quantified protein expressions were normalized by related glyceraldehyde-3-phosphate dehydrogenase. The results are represented as means ± SEM ( n = 4). # P

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Scutellarin Exerts Hypoglycemic and Renal Protective Effects in db/db Mice via the Nrf2/HO-1 Signaling Pathway

    doi: 10.1155/2019/1354345

    Figure Lengend Snippet: Four weeks of SCU treatment increased the expression levels of Nrf2, HO-1, SOD1, SOD2, and CAT in the kidney of db/db mice. The data on quantified protein expressions were normalized by related glyceraldehyde-3-phosphate dehydrogenase. The results are represented as means ± SEM ( n = 4). # P

    Article Snippet: Then, the bands were incubated overnight at 4°C in a corresponding primary antibody solution containing Nrf2 (ab137550), HO-1 (ab13248), SOD1 (ab13498), SOD2 (ab13533), and CAT (ab16731) (1 : 2000; Abcam, UK) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH, ABS16, 1 : 2000, Merck Millipore, USA) and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies (bs-0295G, 1 : 2000, Beijing Biosynthesis Biotechnology Co. Ltd., China) for 4 hours at 4°C.

    Techniques: Expressing, Mouse Assay