glucose tsbg  (Thermo Fisher)


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    Structured Review

    Thermo Fisher glucose tsbg
    Glucose Tsbg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose tsbg/product/Thermo Fisher
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    glucose tsbg - by Bioz Stars, 2020-04
    93/100 stars

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    Related Articles

    Selection:

    Article Title: Enterococcal Metabolite Cues Facilitate Interspecies Niche Modulation and Polymicrobial Infection
    Article Snippet: MacConkey agar was used for E. coli selection and BHI supplemented with colistin at 10µg/ml and nalidixic acid at 10µg/ml for E. faecalis selection. .. For planktonic and biofilm assays, bacteria were cultured at 37°C under 200rpm or static conditions on tryptone soy broth supplemented with 10mM glucose (TSBG) and solidified with 1.5% agar when appropriate (Oxoid Technical No.3).

    Spectrophotometry:

    Article Title: A low molecular weight component of serum inhibits biofilm formation in Staphylococcus aureus
    Article Snippet: Bacteria and biofilms within the wells were resuspended and the A595nm was determined using the plate spectrophotometer. .. Conditions included Tryptic Soy Broth (TSB) supplemented with 1% Glucose (TSBG), TSBG + 50% or 10% Phosphate Buffer Solution (PBS) (GIBCO, Invitrogen, Carlsbad, CA), TSBG + 50% or 10% Fetal Bovine Serum (FBS) (Gemini Bio-Products, West Sacramento, CA) and TSBG + 50% or 10% Human Serum (HS) (MP Biomedicals, Solon, OH).

    Biofilm Production Assay:

    Article Title: A low molecular weight component of serum inhibits biofilm formation in Staphylococcus aureus
    Article Snippet: Paragraph title: 3.1 Biofilm assay ... Conditions included Tryptic Soy Broth (TSB) supplemented with 1% Glucose (TSBG), TSBG + 50% or 10% Phosphate Buffer Solution (PBS) (GIBCO, Invitrogen, Carlsbad, CA), TSBG + 50% or 10% Fetal Bovine Serum (FBS) (Gemini Bio-Products, West Sacramento, CA) and TSBG + 50% or 10% Human Serum (HS) (MP Biomedicals, Solon, OH).

    Cell Culture:

    Article Title: Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism
    Article Snippet: .. For planktonic and biofilm assays, bacteria were cultured at 37°C (under 200-rpm orbital shaking or static conditions, respectively) with tryptone soy broth supplemented with 10 mM glucose (TSBG) and solidified with 1.5% agar when appropriate (Oxoid Technical no. 3). ..

    Article Title: Enterococcal Metabolite Cues Facilitate Interspecies Niche Modulation and Polymicrobial Infection
    Article Snippet: .. For planktonic and biofilm assays, bacteria were cultured at 37°C under 200rpm or static conditions on tryptone soy broth supplemented with 10mM glucose (TSBG) and solidified with 1.5% agar when appropriate (Oxoid Technical No.3). .. Iron depletion was achieved by supplementation with 2,2'dipyridyl (22D) (Sigma Aldrich, St Louis, MO, USA).

    Staining:

    Article Title: A low molecular weight component of serum inhibits biofilm formation in Staphylococcus aureus
    Article Snippet: For one of the replicate plates, the spent medium was removed, the wells were washed with 200μL 1X PBS to remove non-adherent bacteria, and the plates were dried and stained with safranin (PML Microbiologicals, Wilsonville, OR). .. Conditions included Tryptic Soy Broth (TSB) supplemented with 1% Glucose (TSBG), TSBG + 50% or 10% Phosphate Buffer Solution (PBS) (GIBCO, Invitrogen, Carlsbad, CA), TSBG + 50% or 10% Fetal Bovine Serum (FBS) (Gemini Bio-Products, West Sacramento, CA) and TSBG + 50% or 10% Human Serum (HS) (MP Biomedicals, Solon, OH).

    Plasmid Preparation:

    Article Title: Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism
    Article Snippet: For planktonic and biofilm assays, bacteria were cultured at 37°C (under 200-rpm orbital shaking or static conditions, respectively) with tryptone soy broth supplemented with 10 mM glucose (TSBG) and solidified with 1.5% agar when appropriate (Oxoid Technical no. 3). .. E. faecalis strains harboring pMSP3535 (source, Gary M. Dunny; Addgene plasmid 46886) or pMSP3535::ldh1 (source, Axel Hartke [ ]) were selected for with 300 µg/ml erythromycin and induced with 5-µg/ml nisin-supplemented BHI.

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  • 93
    Thermo Fisher tsbg
    SAL decreases the codY transcript level and CodY contributes to diminishing SAL-induced <t>biofilm</t> after iron addition. (A) Biofilms formed by the Newman strain and its isogenic codY mutant in <t>TSBg</t> during 24 h in the presence or absence of 2 mM of SAL with or without 50 μM of FeSO 4 . Each bar represents the arithmetic mean ± SEM of sixtuplicate measurement from 3 independent experiments. The biofilms were quantified by crystal violet staining (OD B ) and expressed relative to the final culture density (OD G ). Comparisons are represented by lines and p -values are indicated above. (B) Expression of codY, icaA , and cap5K transcripts from immature (6 h) and mature (24 h) biofilms formed by the Newman strain grown in the presence or absence of 2 mM of SAL with or without addition of 50 μM of FeSO 4 . Changes in gene expression are shown as normalized mean fold change [2 (−ΔΔCt) ] ± SEM (differences of target gene expression with SAL or SAL+Fe 2+ compared to target gene expression in TSBg). Data were normalized to gyrB expression. Untreated biofilms (TSBg groups) were used as controls (controls = 1, represented by dotted horizontal line). Plain bars: TSBg vs. SAL. Striped bars: TSBg vs. SAL+Fe 2+ . Each bar represents the arithmetic mean ± SEM of 3 independent experiments in duplicate.
    Tsbg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tsbg/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tsbg - by Bioz Stars, 2020-04
    93/100 stars
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    SAL decreases the codY transcript level and CodY contributes to diminishing SAL-induced biofilm after iron addition. (A) Biofilms formed by the Newman strain and its isogenic codY mutant in TSBg during 24 h in the presence or absence of 2 mM of SAL with or without 50 μM of FeSO 4 . Each bar represents the arithmetic mean ± SEM of sixtuplicate measurement from 3 independent experiments. The biofilms were quantified by crystal violet staining (OD B ) and expressed relative to the final culture density (OD G ). Comparisons are represented by lines and p -values are indicated above. (B) Expression of codY, icaA , and cap5K transcripts from immature (6 h) and mature (24 h) biofilms formed by the Newman strain grown in the presence or absence of 2 mM of SAL with or without addition of 50 μM of FeSO 4 . Changes in gene expression are shown as normalized mean fold change [2 (−ΔΔCt) ] ± SEM (differences of target gene expression with SAL or SAL+Fe 2+ compared to target gene expression in TSBg). Data were normalized to gyrB expression. Untreated biofilms (TSBg groups) were used as controls (controls = 1, represented by dotted horizontal line). Plain bars: TSBg vs. SAL. Striped bars: TSBg vs. SAL+Fe 2+ . Each bar represents the arithmetic mean ± SEM of 3 independent experiments in duplicate.

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: SAL decreases the codY transcript level and CodY contributes to diminishing SAL-induced biofilm after iron addition. (A) Biofilms formed by the Newman strain and its isogenic codY mutant in TSBg during 24 h in the presence or absence of 2 mM of SAL with or without 50 μM of FeSO 4 . Each bar represents the arithmetic mean ± SEM of sixtuplicate measurement from 3 independent experiments. The biofilms were quantified by crystal violet staining (OD B ) and expressed relative to the final culture density (OD G ). Comparisons are represented by lines and p -values are indicated above. (B) Expression of codY, icaA , and cap5K transcripts from immature (6 h) and mature (24 h) biofilms formed by the Newman strain grown in the presence or absence of 2 mM of SAL with or without addition of 50 μM of FeSO 4 . Changes in gene expression are shown as normalized mean fold change [2 (−ΔΔCt) ] ± SEM (differences of target gene expression with SAL or SAL+Fe 2+ compared to target gene expression in TSBg). Data were normalized to gyrB expression. Untreated biofilms (TSBg groups) were used as controls (controls = 1, represented by dotted horizontal line). Plain bars: TSBg vs. SAL. Striped bars: TSBg vs. SAL+Fe 2+ . Each bar represents the arithmetic mean ± SEM of 3 independent experiments in duplicate.

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Mutagenesis, Staining, Expressing

    Confocal laser scanning microscopy (CLSM) images of Newman biofilms exposed to SAL or SAL plus iron . Biofilms formed in TSBg with or without SAL or SAL plus iron during 24 h were stained with LIVE/DEAD BacLight stain. Upper and bottom panels show green (live) and red (dead) fluorescing cells, respectively. Sagittal sections of the biofilms are shown below and to the right of each panel. Scale bar: 10 μm. Results are representative of two experiments. By means of COMSTAT2 software analyses of images the maximum thickness (μm) of biofilms and the biomass were determined. Biomass is the amount of biologic material -in volume- present in a given area (μm 3 of sample per μm 2 of covered glass surface).

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: Confocal laser scanning microscopy (CLSM) images of Newman biofilms exposed to SAL or SAL plus iron . Biofilms formed in TSBg with or without SAL or SAL plus iron during 24 h were stained with LIVE/DEAD BacLight stain. Upper and bottom panels show green (live) and red (dead) fluorescing cells, respectively. Sagittal sections of the biofilms are shown below and to the right of each panel. Scale bar: 10 μm. Results are representative of two experiments. By means of COMSTAT2 software analyses of images the maximum thickness (μm) of biofilms and the biomass were determined. Biomass is the amount of biologic material -in volume- present in a given area (μm 3 of sample per μm 2 of covered glass surface).

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Confocal Laser Scanning Microscopy, Staining, Software

    Assessment of extracellular polysaccharide matrix in the Newman strain biofilms. (A) Scanning electron microscopy (SEM) of Newman biofilms grown statically for 24 h at 37°C in TSBg supplemented with 2 mM of SAL or 50 μM of FeSO 4 as indicated. The magnification is 10,000 ×. Arrows show extracellular substances between adjacent cells. (B) Visualization of the extracellular polysaccharide matrix in Newman biofilms by CLSM after staining with green fluorescent labeled WGA. Bacterial cells were stained with the lipophilic membrane red dye FM 4-64. Sagittal sections of the biofilms are shown below and to the right of each panel. Scale bar: 10 μm. Results are representative of two experiments. (C) The effect of Dispersin B treatment on the detachment of the Newman biofilms. Each bar represents the percentage of biofilm formed after the treatments. Lines indicate statistically significant decrease of biofilm formation by cells attached following Dispersin B treatment compared with the untreated biofilms.

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: Assessment of extracellular polysaccharide matrix in the Newman strain biofilms. (A) Scanning electron microscopy (SEM) of Newman biofilms grown statically for 24 h at 37°C in TSBg supplemented with 2 mM of SAL or 50 μM of FeSO 4 as indicated. The magnification is 10,000 ×. Arrows show extracellular substances between adjacent cells. (B) Visualization of the extracellular polysaccharide matrix in Newman biofilms by CLSM after staining with green fluorescent labeled WGA. Bacterial cells were stained with the lipophilic membrane red dye FM 4-64. Sagittal sections of the biofilms are shown below and to the right of each panel. Scale bar: 10 μm. Results are representative of two experiments. (C) The effect of Dispersin B treatment on the detachment of the Newman biofilms. Each bar represents the percentage of biofilm formed after the treatments. Lines indicate statistically significant decrease of biofilm formation by cells attached following Dispersin B treatment compared with the untreated biofilms.

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Electron Microscopy, Confocal Laser Scanning Microscopy, Staining, Labeling, Whole Genome Amplification

    Capsular polysaccharide (CP) expression in biofilm formed by the Newman strain . Biofilms were developed in static cultures for 24 h at 37°C in TSBg supplemented with SAL or FeSO 4 as indicated. CP serotype 5 was assessed by ELISA. Each bar represents the arithmetic mean ± SEM of the Abs 492 relative to the final culture density (OD G ). Negative control: S. aureus Reynolds CP5-null.

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: Capsular polysaccharide (CP) expression in biofilm formed by the Newman strain . Biofilms were developed in static cultures for 24 h at 37°C in TSBg supplemented with SAL or FeSO 4 as indicated. CP serotype 5 was assessed by ELISA. Each bar represents the arithmetic mean ± SEM of the Abs 492 relative to the final culture density (OD G ). Negative control: S. aureus Reynolds CP5-null.

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Negative Control

    Interplay of codY and SAL in biofilms. (A) The surface glycopolymer composition was examined by FTIR spectroscopy. PCA was carried out on second derivative, vector-normalized FTIR spectra in the spectral range of 1200–800 cm −1 derived from biofilm produced by the strain Newman and its isogenic codY mutant. Bacteria were grown in TSBg in the presence or absence of 2 mM of SAL with or without 50 μM of FeSO 4 . Clusters are indicated with ellipses. Symbols are defined in the figure. The relative contribution of each PC is indicated in parentheses. (B) PIA production by the Newman codY mutant in biofilms was assessed by fluorometric assays. Each bar represents the arithmetic mean ± SEM of quadruplicate measurements from 3 independent experiments. The values of fluorescence (F) were related to the OD of growth (OD G ) in each well. Comparisons are represented by lines and p -values are denoted above. Data from the SA113 and Newman ica -deficient mutants were included as positive and negative controls, respectively.

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: Interplay of codY and SAL in biofilms. (A) The surface glycopolymer composition was examined by FTIR spectroscopy. PCA was carried out on second derivative, vector-normalized FTIR spectra in the spectral range of 1200–800 cm −1 derived from biofilm produced by the strain Newman and its isogenic codY mutant. Bacteria were grown in TSBg in the presence or absence of 2 mM of SAL with or without 50 μM of FeSO 4 . Clusters are indicated with ellipses. Symbols are defined in the figure. The relative contribution of each PC is indicated in parentheses. (B) PIA production by the Newman codY mutant in biofilms was assessed by fluorometric assays. Each bar represents the arithmetic mean ± SEM of quadruplicate measurements from 3 independent experiments. The values of fluorescence (F) were related to the OD of growth (OD G ) in each well. Comparisons are represented by lines and p -values are denoted above. Data from the SA113 and Newman ica -deficient mutants were included as positive and negative controls, respectively.

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Spectroscopy, Plasmid Preparation, Derivative Assay, Produced, Mutagenesis, Fluorescence

    The effect of SAL on biofilm formed by MRSA and MSSA strains . Biofilms were formed in TSBg during 24 h in the presence or absence of 2 mM of SAL or SAL plus 50 μM of FeSO 4 . Each bar represents the arithmetic mean ± SEM of 6 wells from 4 independent experiments. The biofilms were quantified by crystal violet staining (OD B ) and expressed relative to the final culture density (OD G ). Statistically significant differences were represented by asterisks: ( * ) SAL-treated group vs. untreated group; ( ** ) SAL-treated group vs. SAL plus iron-treated group. p

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: The effect of SAL on biofilm formed by MRSA and MSSA strains . Biofilms were formed in TSBg during 24 h in the presence or absence of 2 mM of SAL or SAL plus 50 μM of FeSO 4 . Each bar represents the arithmetic mean ± SEM of 6 wells from 4 independent experiments. The biofilms were quantified by crystal violet staining (OD B ) and expressed relative to the final culture density (OD G ). Statistically significant differences were represented by asterisks: ( * ) SAL-treated group vs. untreated group; ( ** ) SAL-treated group vs. SAL plus iron-treated group. p

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Staining

    Impact of SAL and SAL plus iron on S. aureus monitored by FTIR spectroscopy . PCA was carried out on second derivative, vector-normalized FTIR spectra in the spectral range for glycopolymers (1200–800 cm −1 ). (A) Newman strain, planktonic culture. (B) Biofilm formed by the Newman strain. In both cases bacteria were grown in TSBg with or without 2 mM of SAL or SAL plus 50 μM FeSO 4 . Clusters are indicated with ellipses. Symbols are defined in the figure. The relative contribution of each principal component is indicated in parentheses.

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: Impact of SAL and SAL plus iron on S. aureus monitored by FTIR spectroscopy . PCA was carried out on second derivative, vector-normalized FTIR spectra in the spectral range for glycopolymers (1200–800 cm −1 ). (A) Newman strain, planktonic culture. (B) Biofilm formed by the Newman strain. In both cases bacteria were grown in TSBg with or without 2 mM of SAL or SAL plus 50 μM FeSO 4 . Clusters are indicated with ellipses. Symbols are defined in the figure. The relative contribution of each principal component is indicated in parentheses.

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Spectroscopy, Plasmid Preparation

    SAL chelating capacity of iron. (A) Standard curve of ferrous ion concentration in ferrozine complexes determined in TSBg medium using 1.25 mM ferrozine. (B) Ferrozine-Fe 2+ complex quantification in TSBg treated with different concentrations of SAL mixed with 50 μM FeSO 4 . The amount of ferrozine-Fe 2+ complex was determined at an absorbance of 540 nm (Abs 540 ). Basal iron concentration in TSBg was 39.3 μM.

    Journal: Frontiers in Microbiology

    Article Title: The Active Component of Aspirin, Salicylic Acid, Promotes Staphylococcus aureus Biofilm Formation in a PIA-dependent Manner

    doi: 10.3389/fmicb.2017.00004

    Figure Lengend Snippet: SAL chelating capacity of iron. (A) Standard curve of ferrous ion concentration in ferrozine complexes determined in TSBg medium using 1.25 mM ferrozine. (B) Ferrozine-Fe 2+ complex quantification in TSBg treated with different concentrations of SAL mixed with 50 μM FeSO 4 . The amount of ferrozine-Fe 2+ complex was determined at an absorbance of 540 nm (Abs 540 ). Basal iron concentration in TSBg was 39.3 μM.

    Article Snippet: Biofilm visualization by microscopy Overnight cultures of S. aureus grown in TSBg, TSBg with SAL or TSBg with SAL plus iron were adjusted to an OD600 of 0.05 and aliquots were utilized to inoculate the wells of a 24-well plate (scanning electron microscopy, SEM) or a 8-well chambered coverglass (ThermoFisher Scientific, Waltham, MA, USA) (confocal laser scanning microscopy, CLSM).

    Techniques: Concentration Assay