glucose dulbecco s modified eagle s medium  (Thermo Fisher)


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    Name:
    DMEM high glucose
    Description:
    DMEM Dulbecco s Modified Eagle Medium is a widely used basal medium for supporting the growth of many different mammalian cells Cells successfully cultured in DMEM include primary fibroblasts neurons glial cells HUVECs and smooth muscle cells as well as cell lines such as HeLa 293 Cos 7 and PC 12 Life Technologies offers a variety of DMEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This DMEM is modified as follows WithWithout• High Glucose• Sodium Pyruvate• L glutamine• HEPES• Phenol RedThe complete formulation is available Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle s Minimal Essential Medium DMEM was originally formulated with low glucose 1 g L and sodium pyruvate but is often used with higher glucose levels with or without sodium pyruvate DMEM contains no proteins lipids or growth factors Therefore DMEM requires supplementation commonly with 10 Fetal Bovine Serum FBS DMEM uses a sodium bicarbonate buffer system 3 7 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH Product useFor human ex vivo tissue and cell culture processing applications CAUTION When used as a medical device Federal law restricts this device to sale by or on the order of a physician Customers using Gibco DMEM in a manufacturing process who have a submission with the FDA may request a letter of authorization from Life Technologies to reference our Type II Drug Master File DMF cGMP manufacturing and quality systemDMEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity Life Technologies offers an identical DMEM product made in our Scotland facility 41965 039 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
    Catalog Number:
    11965084
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher glucose dulbecco s modified eagle s medium
    DMEM Dulbecco s Modified Eagle Medium is a widely used basal medium for supporting the growth of many different mammalian cells Cells successfully cultured in DMEM include primary fibroblasts neurons glial cells HUVECs and smooth muscle cells as well as cell lines such as HeLa 293 Cos 7 and PC 12 Life Technologies offers a variety of DMEM modifications for a range of cell culture applications Find the right formulation using the media selector tool This DMEM is modified as follows WithWithout• High Glucose• Sodium Pyruvate• L glutamine• HEPES• Phenol RedThe complete formulation is available Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle s Minimal Essential Medium DMEM was originally formulated with low glucose 1 g L and sodium pyruvate but is often used with higher glucose levels with or without sodium pyruvate DMEM contains no proteins lipids or growth factors Therefore DMEM requires supplementation commonly with 10 Fetal Bovine Serum FBS DMEM uses a sodium bicarbonate buffer system 3 7 g L and therefore requires a 5 10 CO2 environment to maintain physiological pH Product useFor human ex vivo tissue and cell culture processing applications CAUTION When used as a medical device Federal law restricts this device to sale by or on the order of a physician Customers using Gibco DMEM in a manufacturing process who have a submission with the FDA may request a letter of authorization from Life Technologies to reference our Type II Drug Master File DMF cGMP manufacturing and quality systemDMEM is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards For supply chain continuity Life Technologies offers an identical DMEM product made in our Scotland facility 41965 039 This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard
    https://www.bioz.com/result/glucose dulbecco s modified eagle s medium/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glucose dulbecco s modified eagle s medium - by Bioz Stars, 2021-03
    97/100 stars

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    Related Articles

    Transduction:

    Article Title: Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype
    Article Snippet: Culture of primary cells and cell lines EpCAMhigh /CD271low /CD166high /CD117low ER-positive cells were purified from normal breast as previously described [ ]. .. Cells transduced with hTERT/shp16 in early passage [ ] were cultured in Primaria (#3813, Becton Dickenson) in the presence of TGFβR2i medium (Dulbecco's modified Eagle's medium (DMEM, high glucose, no calcium, Life Technologies):Ham's F12 Nutrient Mixture (F12, Life Technologies), 3:1 v/v), 0.5 μg/ml hydrocortisone, 5 μg/ml insulin, 10 ng/ml cholera toxin (Sigma-Aldrich), 10 ng/ml epidermal growth factor (Peprotech), 1.8 x10-4 M adenine (Sigma Aldrich), 10 μM Y-27632 (Axon Medchem) and 5% fetal bovine serum (Sigma Aldrich), with the addition of the selective inhibitor of TGF-β type I receptor activin receptor-like kinase ALK5, ALK4 and ALK7, SB431542 (10 μM, Axon 1661, Axon Medchem) and an inhibitor of autophosphorylation of ALK-5, RepSox (25-50μM, R0158, Sigma Aldrich) [ ]). .. To restrict the luminal phenotype, in 6th and again in 11th passage, CD146high cells were purified by FACS (P1H12 1:500, ab24577, Abcam, as primary antibody and goat anti-mouse IgG1 Alexa Flour 647, 1:500, Life Technologies as secondary), and in 22nd passage CD146high /CD117high cells (CD146, 1:20, BD Biosciences followed by IgG1 Alexa Flour 647 and CD117, 104D2-PE (1:20)) were sorted.

    Cell Culture:

    Article Title: Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype
    Article Snippet: Culture of primary cells and cell lines EpCAMhigh /CD271low /CD166high /CD117low ER-positive cells were purified from normal breast as previously described [ ]. .. Cells transduced with hTERT/shp16 in early passage [ ] were cultured in Primaria (#3813, Becton Dickenson) in the presence of TGFβR2i medium (Dulbecco's modified Eagle's medium (DMEM, high glucose, no calcium, Life Technologies):Ham's F12 Nutrient Mixture (F12, Life Technologies), 3:1 v/v), 0.5 μg/ml hydrocortisone, 5 μg/ml insulin, 10 ng/ml cholera toxin (Sigma-Aldrich), 10 ng/ml epidermal growth factor (Peprotech), 1.8 x10-4 M adenine (Sigma Aldrich), 10 μM Y-27632 (Axon Medchem) and 5% fetal bovine serum (Sigma Aldrich), with the addition of the selective inhibitor of TGF-β type I receptor activin receptor-like kinase ALK5, ALK4 and ALK7, SB431542 (10 μM, Axon 1661, Axon Medchem) and an inhibitor of autophosphorylation of ALK-5, RepSox (25-50μM, R0158, Sigma Aldrich) [ ]). .. To restrict the luminal phenotype, in 6th and again in 11th passage, CD146high cells were purified by FACS (P1H12 1:500, ab24577, Abcam, as primary antibody and goat anti-mouse IgG1 Alexa Flour 647, 1:500, Life Technologies as secondary), and in 22nd passage CD146high /CD117high cells (CD146, 1:20, BD Biosciences followed by IgG1 Alexa Flour 647 and CD117, 104D2-PE (1:20)) were sorted.

    Article Title: Formin DAAM1 Organizes Actin Filaments in the Cytoplasmic Nodal Actin Network
    Article Snippet: GFP-β-actin was purchased from CLONTECH Laboratories. .. Cell culture and transfection Immortalized MEFs [ ], Human foreskin fibroblasts (HFFs) (ATCC® SCRC-1041™ , purchased from American Type Culture Collection (ATCC)) and HeLa JW cells [ ] were maintained in DMEM high glucose medium (Gibco) supplemented with 10% fetal bovine serum (Gibco), 1% L-glutamine, 1 mM sodium pyruvate and 100IU/mg penicillin-streptomycin (Invitrogen) at 37°C and 5% CO2 . .. Human large cell lung cancer cell line H460 (ATCC® HTB-177™ , purchased from ATCC) were maintained in ATCC-formulated RPMI-1640 Medium (Catalog No. 30–2001).

    Article Title: New insights in gene expression alteration as effect of doxorubicin drug resistance in triple negative breast cancer cells
    Article Snippet: Cell culture and induction of doxorubicin resistance In this study, the experiments were performed on triple negative breast cancer (TNBC) cell lines, Hs578T and MDA-MB-231. .. The Hs578T cell line was cultured in D-MEM high glucose (D-MEM Gibco®) supplemented with 10% fetal bovine serum (FBS- Gibco®), 2 mM L-glutamine (Gibco®), 1% MEM Non-Essential Amino Acids Solution (100X, Gibco®), 0.01 mg/ml insulin and 1% Penicillin-Streptomycin (Gibco®). .. MDA-MB-231 cell line was cultured in RPMI-1640 (RPMI-1640 Gibco®), supplemented with 10% fetal bovine serum (FBS- Gibco®), 2 mM L-glutamine (Gibco®) and 1% Penicillin-Streptomycin (Gibco®).

    Article Title: Measuring the density and viscosity of culture media for optimized computational fluid dynamics analysis of in vitro devices
    Article Snippet: Overall, these results recommend the use of experimen characterizing model-specific fluid properties of model-specific culture media for optimized design and more accurate CFD analysis of in vitro culture systems. .. 2.1 Sample preparation & cell culture 10mL samples of RPMI-1640 (Gibco, Thermo Fisher Scientific) and DMEM high glucose medium (11965-092, Gibco, Thermo Fisher Scientific) supplemented with 0, 5, 10 and 20 % v/v FBS (10091148, Gibco, Thermo Fisher Scientific) were dispensed into 10mL capped tubes. .. To evaluate the changes in the density and viscosity of culture medium during cell culture, 10 mL samples were extracted during routine passaging of NCI-H460 and HN6 cells after 3 days of culture.

    Modification:

    Article Title: Establishment of a normal-derived estrogen receptor-positive cell line comparable to the prevailing human breast cancer subtype
    Article Snippet: Culture of primary cells and cell lines EpCAMhigh /CD271low /CD166high /CD117low ER-positive cells were purified from normal breast as previously described [ ]. .. Cells transduced with hTERT/shp16 in early passage [ ] were cultured in Primaria (#3813, Becton Dickenson) in the presence of TGFβR2i medium (Dulbecco's modified Eagle's medium (DMEM, high glucose, no calcium, Life Technologies):Ham's F12 Nutrient Mixture (F12, Life Technologies), 3:1 v/v), 0.5 μg/ml hydrocortisone, 5 μg/ml insulin, 10 ng/ml cholera toxin (Sigma-Aldrich), 10 ng/ml epidermal growth factor (Peprotech), 1.8 x10-4 M adenine (Sigma Aldrich), 10 μM Y-27632 (Axon Medchem) and 5% fetal bovine serum (Sigma Aldrich), with the addition of the selective inhibitor of TGF-β type I receptor activin receptor-like kinase ALK5, ALK4 and ALK7, SB431542 (10 μM, Axon 1661, Axon Medchem) and an inhibitor of autophosphorylation of ALK-5, RepSox (25-50μM, R0158, Sigma Aldrich) [ ]). .. To restrict the luminal phenotype, in 6th and again in 11th passage, CD146high cells were purified by FACS (P1H12 1:500, ab24577, Abcam, as primary antibody and goat anti-mouse IgG1 Alexa Flour 647, 1:500, Life Technologies as secondary), and in 22nd passage CD146high /CD117high cells (CD146, 1:20, BD Biosciences followed by IgG1 Alexa Flour 647 and CD117, 104D2-PE (1:20)) were sorted.

    Article Title: New Mammalian Target of Rapamycin (mTOR) Modulators Derived from Natural Product Databases and Marine Extracts by Using Molecular Docking Techniques
    Article Snippet: Cell Culture and Treatment Rapamycin and MTT were purchased from Sigma-Aldrich, St. Louis, MO, USA. .. High-glucose Dulbecco’s Modified Eagle’s Medium, penicillin-streptomycin, fetal bovine serum and 0.05x trypsin/ethylene diamine tetra acetic acid was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). .. The human colorectal carcinoma cell line HCT116 was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was maintained in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% heat inactivated fetal bovine serum (FBS), 100 U/mL of penicillin and 100 g/mL of streptomycin.

    Transfection:

    Article Title: Formin DAAM1 Organizes Actin Filaments in the Cytoplasmic Nodal Actin Network
    Article Snippet: GFP-β-actin was purchased from CLONTECH Laboratories. .. Cell culture and transfection Immortalized MEFs [ ], Human foreskin fibroblasts (HFFs) (ATCC® SCRC-1041™ , purchased from American Type Culture Collection (ATCC)) and HeLa JW cells [ ] were maintained in DMEM high glucose medium (Gibco) supplemented with 10% fetal bovine serum (Gibco), 1% L-glutamine, 1 mM sodium pyruvate and 100IU/mg penicillin-streptomycin (Invitrogen) at 37°C and 5% CO2 . .. Human large cell lung cancer cell line H460 (ATCC® HTB-177™ , purchased from ATCC) were maintained in ATCC-formulated RPMI-1640 Medium (Catalog No. 30–2001).

    Derivative Assay:

    Article Title: CACNB2 Is a Novel Susceptibility Gene for Diabetic Retinopathy in Type 1 Diabetes
    Article Snippet: ARPE19 cells were grown in DMEM-F12 (D6421; Sigma-Aldrich) supplemented with 10% FBS (10270106; Gibco/Life Technologies), penicillin-streptomycin (15140122; Gibco/Life Technologies), GlutaMAX Supplement (35050061; Gibco/Life Technologies), and Normocin (ant-nr-1; InvivoGen). .. MIO-M1 cells (Müller glial cell lines derived from adult human retina) were obtained from Limb’s laboratory ( ) and grown in DMEM (11965092; Thermo Fisher Scientific), otherwise similarly to ARPE19 cells. ..

    Sample Prep:

    Article Title: Measuring the density and viscosity of culture media for optimized computational fluid dynamics analysis of in vitro devices
    Article Snippet: Overall, these results recommend the use of experimen characterizing model-specific fluid properties of model-specific culture media for optimized design and more accurate CFD analysis of in vitro culture systems. .. 2.1 Sample preparation & cell culture 10mL samples of RPMI-1640 (Gibco, Thermo Fisher Scientific) and DMEM high glucose medium (11965-092, Gibco, Thermo Fisher Scientific) supplemented with 0, 5, 10 and 20 % v/v FBS (10091148, Gibco, Thermo Fisher Scientific) were dispensed into 10mL capped tubes. .. To evaluate the changes in the density and viscosity of culture medium during cell culture, 10 mL samples were extracted during routine passaging of NCI-H460 and HN6 cells after 3 days of culture.

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    Thermo Fisher high glucose dmem
    CD36 regulates AMPK activation via Fyn-dependent nuclear sequestration of LKB1. A : Suppression of AMPK phosphorylation by CD36 expression. CHO cells lacking CD36 (Vector) or stably expressing CD36 (CHO-CD36) were serum starved (for 16 h in <t>DMEM</t> buffer A), lysed, and probed (in triplicate) for CD36, pAMPK (T172), and GAPDH. Data are representative of two experiments. B : Fyn phosphorylation of LKB1 is enhanced by CD36 expression. Control (Vector) and CD36-expressing cells (CD36) were transiently transfected with Fyn or FynD; and serum-starved and cell lysates (Input) were probed for CD36, LKB1, and Fyn. LKB1 was immunoprecipitated from cell lysates using mouse monoclonal antibody for LKB1 (clone 5c10; Millipore). LKB1 immunoprecipitates (IP:LKB1) were probed with phosphotyrosine PY100 (pLKB1) and LKB1 antibodies. C : CD36 expression induces nuclear sequestration of LKB1 in CHO cells. CHO cells stably expressing CD36 or Vector controls were serum starved, fixed, and processed for IF as described in Research Design and Methods . The cells were stained with mouse monoclonal LKB1 antibody (clone 5c10; Millipore) and with DAPI to visualize the nuclei. Images are representative of multiple fields from three experiments. Scale bar, 10 μm. D : CD36 depletion in <t>C2C12</t> myotubes abolishes nuclear sequestration of LKB1. C2C12 myotubes treated with siCD36 or siCont were serum starved for 16 h in DMEM buffer A. LKB1 and CD36 were detected using mouse monoclonal anti-LKB1 (5c10; Millipore) and rat monoclonal anti-CD36 antibodies (MF3; AbD Serotec). Images are representative of multiple fields from three experiments. Scale bar, 10 μm.
    High Glucose Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high glucose dmem/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
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    99
    Thermo Fisher low glucose dulbecco s modified eagle s medium dmem
    Expression of cardiac structural proteins by MSCs in vivo, determined by immunofluorescence. (A and E) Positive staining for cTnI and α-sarcomeric actin protein of MSCs three weeks following transplantation into an ischemic environment (red fluorescence; magnification, ×40). (B and F) DAPI-labeled nuclei of MSCs three weeks following transplantation (blue fluorescence; magnification, ×40). (C) Merged image of A and B. (G) Merged image of E and F (magnification, ×40). (D and H) Negative staining for cTnI and α-sarcomeric actin protein in myocardial infarction of <t>Dulbecco’s</t> modified <t>Eagle’s</t> medium group. MSCs, mesenchymal stem cells; cTnI, cardiac troponin I.
    Low Glucose Dulbecco S Modified Eagle S Medium Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/low glucose dulbecco s modified eagle s medium dmem/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
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    99
    Thermo Fisher glucose l glutamine dulbecco s modified eagle s medium
    siRNA knockdown of α-AP-2 reduces CFTR endocytosis. A , HEK 293 cells expressing CFTR were incubated overnight in <t>Dulbecco's</t> modified <t>Eagle's</t> medium with 10% fetal bovine serum and allowed to grow to 70% confluency. The cells were transfected with α-AP-2 or scrambled siRNA and analyzed by Western blot at 72 h to detect α-AP-2 and β-actin ( A ). B , cell surface proteins were labeled using NHS SS-biotin and allowed to internalize for 1 and 5 min at 37 °C. Following MESNA treatment, the cells were lysed, surface biotinylated proteins were bound to streptavidin-agarose beads, and CFTR was detected by Western blot. C , the percentage of cell surface CFTR internalized over time is shown. The data are expressed as the means ± S.E. ( n > 3).
    Glucose L Glutamine Dulbecco S Modified Eagle S Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glucose l glutamine dulbecco s modified eagle s medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    CD36 regulates AMPK activation via Fyn-dependent nuclear sequestration of LKB1. A : Suppression of AMPK phosphorylation by CD36 expression. CHO cells lacking CD36 (Vector) or stably expressing CD36 (CHO-CD36) were serum starved (for 16 h in DMEM buffer A), lysed, and probed (in triplicate) for CD36, pAMPK (T172), and GAPDH. Data are representative of two experiments. B : Fyn phosphorylation of LKB1 is enhanced by CD36 expression. Control (Vector) and CD36-expressing cells (CD36) were transiently transfected with Fyn or FynD; and serum-starved and cell lysates (Input) were probed for CD36, LKB1, and Fyn. LKB1 was immunoprecipitated from cell lysates using mouse monoclonal antibody for LKB1 (clone 5c10; Millipore). LKB1 immunoprecipitates (IP:LKB1) were probed with phosphotyrosine PY100 (pLKB1) and LKB1 antibodies. C : CD36 expression induces nuclear sequestration of LKB1 in CHO cells. CHO cells stably expressing CD36 or Vector controls were serum starved, fixed, and processed for IF as described in Research Design and Methods . The cells were stained with mouse monoclonal LKB1 antibody (clone 5c10; Millipore) and with DAPI to visualize the nuclei. Images are representative of multiple fields from three experiments. Scale bar, 10 μm. D : CD36 depletion in C2C12 myotubes abolishes nuclear sequestration of LKB1. C2C12 myotubes treated with siCD36 or siCont were serum starved for 16 h in DMEM buffer A. LKB1 and CD36 were detected using mouse monoclonal anti-LKB1 (5c10; Millipore) and rat monoclonal anti-CD36 antibodies (MF3; AbD Serotec). Images are representative of multiple fields from three experiments. Scale bar, 10 μm.

    Journal: Diabetes

    Article Title: Regulation of AMPK Activation by CD36 Links Fatty Acid Uptake to β-Oxidation

    doi: 10.2337/db14-0582

    Figure Lengend Snippet: CD36 regulates AMPK activation via Fyn-dependent nuclear sequestration of LKB1. A : Suppression of AMPK phosphorylation by CD36 expression. CHO cells lacking CD36 (Vector) or stably expressing CD36 (CHO-CD36) were serum starved (for 16 h in DMEM buffer A), lysed, and probed (in triplicate) for CD36, pAMPK (T172), and GAPDH. Data are representative of two experiments. B : Fyn phosphorylation of LKB1 is enhanced by CD36 expression. Control (Vector) and CD36-expressing cells (CD36) were transiently transfected with Fyn or FynD; and serum-starved and cell lysates (Input) were probed for CD36, LKB1, and Fyn. LKB1 was immunoprecipitated from cell lysates using mouse monoclonal antibody for LKB1 (clone 5c10; Millipore). LKB1 immunoprecipitates (IP:LKB1) were probed with phosphotyrosine PY100 (pLKB1) and LKB1 antibodies. C : CD36 expression induces nuclear sequestration of LKB1 in CHO cells. CHO cells stably expressing CD36 or Vector controls were serum starved, fixed, and processed for IF as described in Research Design and Methods . The cells were stained with mouse monoclonal LKB1 antibody (clone 5c10; Millipore) and with DAPI to visualize the nuclei. Images are representative of multiple fields from three experiments. Scale bar, 10 μm. D : CD36 depletion in C2C12 myotubes abolishes nuclear sequestration of LKB1. C2C12 myotubes treated with siCD36 or siCont were serum starved for 16 h in DMEM buffer A. LKB1 and CD36 were detected using mouse monoclonal anti-LKB1 (5c10; Millipore) and rat monoclonal anti-CD36 antibodies (MF3; AbD Serotec). Images are representative of multiple fields from three experiments. Scale bar, 10 μm.

    Article Snippet: Cells C2C12 myoblasts grown in high-glucose DMEM with 10% FBS (Life Technologies) supplemented with l -glutamine (2 mmol/L) were differentiated in DMEM containing 2% horse serum.

    Techniques: Activation Assay, Expressing, Plasmid Preparation, Stable Transfection, Transfection, Immunoprecipitation, Staining

    Expression of cardiac structural proteins by MSCs in vivo, determined by immunofluorescence. (A and E) Positive staining for cTnI and α-sarcomeric actin protein of MSCs three weeks following transplantation into an ischemic environment (red fluorescence; magnification, ×40). (B and F) DAPI-labeled nuclei of MSCs three weeks following transplantation (blue fluorescence; magnification, ×40). (C) Merged image of A and B. (G) Merged image of E and F (magnification, ×40). (D and H) Negative staining for cTnI and α-sarcomeric actin protein in myocardial infarction of Dulbecco’s modified Eagle’s medium group. MSCs, mesenchymal stem cells; cTnI, cardiac troponin I.

    Journal: Molecular Medicine Reports

    Article Title: Nesprin-1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte-like cells in vivo and in vitro

    doi: 10.3892/mmr.2014.2754

    Figure Lengend Snippet: Expression of cardiac structural proteins by MSCs in vivo, determined by immunofluorescence. (A and E) Positive staining for cTnI and α-sarcomeric actin protein of MSCs three weeks following transplantation into an ischemic environment (red fluorescence; magnification, ×40). (B and F) DAPI-labeled nuclei of MSCs three weeks following transplantation (blue fluorescence; magnification, ×40). (C) Merged image of A and B. (G) Merged image of E and F (magnification, ×40). (D and H) Negative staining for cTnI and α-sarcomeric actin protein in myocardial infarction of Dulbecco’s modified Eagle’s medium group. MSCs, mesenchymal stem cells; cTnI, cardiac troponin I.

    Article Snippet: Cell culture mediums, low-glucose Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS), were purchased from Gibco-BRL.

    Techniques: Expressing, In Vivo, Immunofluorescence, Staining, Transplantation Assay, Fluorescence, Labeling, Negative Staining, Modification

    Expression levels of nesprin-1 protein detected by western blot analysis. (A) Expression levels of nesprin-1 protein in the 5-azacytidine-treated MSCs are higher than those of the untreated MSCs. (B) Nesprin-1 protein expression levels were higher in the MSC group than those in the DMEM control group, but lower than those in the normal group. MSCs, mesenchymal stem cells; DMEM, Dulbecco’s modified Eagle’s medium; ind-msc, 5-azacytidine-treated MSCs; Ind-msc, 5-azacytidine-treated MSCs.

    Journal: Molecular Medicine Reports

    Article Title: Nesprin-1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte-like cells in vivo and in vitro

    doi: 10.3892/mmr.2014.2754

    Figure Lengend Snippet: Expression levels of nesprin-1 protein detected by western blot analysis. (A) Expression levels of nesprin-1 protein in the 5-azacytidine-treated MSCs are higher than those of the untreated MSCs. (B) Nesprin-1 protein expression levels were higher in the MSC group than those in the DMEM control group, but lower than those in the normal group. MSCs, mesenchymal stem cells; DMEM, Dulbecco’s modified Eagle’s medium; ind-msc, 5-azacytidine-treated MSCs; Ind-msc, 5-azacytidine-treated MSCs.

    Article Snippet: Cell culture mediums, low-glucose Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS), were purchased from Gibco-BRL.

    Techniques: Expressing, Western Blot, Modification

    Immunofluorescence to identify the expression of nesprin-1 protein. (A) MSCs positive for nesprin-1 protein four weeks following treatment with 5-azacytidine and (B) nesprin-1 protein expression of untreated MSCs following four weeks of culture (green fluorescence; magnification, ×400; ** P=0.0032 vs. untreated group). (C) MSCs positive for nesprin-1 protein three weeks following transplant into an ischemic environment (red fluorescence; magnification, ×40). (D) DAPI-labeled nuclei of transplanted MSCs three weeks following transplantation (blue fluorescence; magnification, ×40). (E) Merged image of C and D (magnification, ×40). (F) Negative staining for nesprin-1 protein in myocardial infarction of Dulbecco’s modified Eagle’s medium group. MSCs, mesenchymal stem cells; Ind-msc, 5-azacytidine-treated MSCs.

    Journal: Molecular Medicine Reports

    Article Title: Nesprin-1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte-like cells in vivo and in vitro

    doi: 10.3892/mmr.2014.2754

    Figure Lengend Snippet: Immunofluorescence to identify the expression of nesprin-1 protein. (A) MSCs positive for nesprin-1 protein four weeks following treatment with 5-azacytidine and (B) nesprin-1 protein expression of untreated MSCs following four weeks of culture (green fluorescence; magnification, ×400; ** P=0.0032 vs. untreated group). (C) MSCs positive for nesprin-1 protein three weeks following transplant into an ischemic environment (red fluorescence; magnification, ×40). (D) DAPI-labeled nuclei of transplanted MSCs three weeks following transplantation (blue fluorescence; magnification, ×40). (E) Merged image of C and D (magnification, ×40). (F) Negative staining for nesprin-1 protein in myocardial infarction of Dulbecco’s modified Eagle’s medium group. MSCs, mesenchymal stem cells; Ind-msc, 5-azacytidine-treated MSCs.

    Article Snippet: Cell culture mediums, low-glucose Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS), were purchased from Gibco-BRL.

    Techniques: Immunofluorescence, Expressing, Fluorescence, Labeling, Transplantation Assay, Negative Staining, Modification

    Bone marrow mesenchymal stem cell viability was assessed by MTT assay after 24 h of exposure to low-glucose DMEM supplemented with 1, 10, 50, 100, 200, 500 and 1,000 µM PVP-I. DMEM represents the PVP-I-free control group. DMEM, Dulbecco's modified Eagle's medium; PVP-I, polyvinylpyrrolidone-iodine.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effects of polyvinylpyrrolidone-iodine on tendon-bone healing in a rabbit extra-articular model

    doi: 10.3892/etm.2017.4359

    Figure Lengend Snippet: Bone marrow mesenchymal stem cell viability was assessed by MTT assay after 24 h of exposure to low-glucose DMEM supplemented with 1, 10, 50, 100, 200, 500 and 1,000 µM PVP-I. DMEM represents the PVP-I-free control group. DMEM, Dulbecco's modified Eagle's medium; PVP-I, polyvinylpyrrolidone-iodine.

    Article Snippet: In brief, 2 ml bone marrow was obtained, mixed with 2 ml low-glucose Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc., Waltham, MA, USA) and centrifuged at 1,200 × g for 5 min.

    Techniques: MTT Assay, Modification

    siRNA knockdown of α-AP-2 reduces CFTR endocytosis. A , HEK 293 cells expressing CFTR were incubated overnight in Dulbecco's modified Eagle's medium with 10% fetal bovine serum and allowed to grow to 70% confluency. The cells were transfected with α-AP-2 or scrambled siRNA and analyzed by Western blot at 72 h to detect α-AP-2 and β-actin ( A ). B , cell surface proteins were labeled using NHS SS-biotin and allowed to internalize for 1 and 5 min at 37 °C. Following MESNA treatment, the cells were lysed, surface biotinylated proteins were bound to streptavidin-agarose beads, and CFTR was detected by Western blot. C , the percentage of cell surface CFTR internalized over time is shown. The data are expressed as the means ± S.E. ( n > 3).

    Journal: The Journal of Biological Chemistry

    Article Title: ?-AP-2 Directs Myosin VI-dependent Endocytosis of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channels in the Intestine *

    doi: 10.1074/jbc.M110.127613

    Figure Lengend Snippet: siRNA knockdown of α-AP-2 reduces CFTR endocytosis. A , HEK 293 cells expressing CFTR were incubated overnight in Dulbecco's modified Eagle's medium with 10% fetal bovine serum and allowed to grow to 70% confluency. The cells were transfected with α-AP-2 or scrambled siRNA and analyzed by Western blot at 72 h to detect α-AP-2 and β-actin ( A ). B , cell surface proteins were labeled using NHS SS-biotin and allowed to internalize for 1 and 5 min at 37 °C. Following MESNA treatment, the cells were lysed, surface biotinylated proteins were bound to streptavidin-agarose beads, and CFTR was detected by Western blot. C , the percentage of cell surface CFTR internalized over time is shown. The data are expressed as the means ± S.E. ( n > 3).

    Article Snippet: The cells were grown in high glucose l -glutamine Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Invitrogen) and hygromycin B (150 μg/ml; Invitrogen) at 37 °C in 5% CO2 , 90% air atmosphere.

    Techniques: Expressing, Incubation, Modification, Transfection, Western Blot, Labeling