Glibenclamide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells"
Article Title: Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells
Figure Legend Snippet: Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM glibenclamide and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.
Techniques Used: Concentration Assay, Activation Assay, Blocking Assay
Figure Legend Snippet: The contribution of various current components (K Ca , K ATP ) and the action of gambierol (100 nM) on total K + current of rat fetal AMC cells. The K + current was recorded using the perforated whole-cell voltage-clamp technique, during 90 ms depolarizing steps from a holding potential of −70 mV, under the conditions indicated in the figure ( A , B ). Note in ( A , B ) the different sequences of gambierol addition to the external medium. ( a ) Superimposed traces of outward K + currents recorded under control conditions and after the perfusion of the different pharmacological agents indicated. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin. ( b ) Current-voltage relationships. The current was measured at the end of the depolarizing steps and expressed as a percentage of control values for the indicated agents. ( c ) Relative outward K + current contribution (expressed as a percentage of the total current) for the pharmacological treatments indicated. The K + current was measured at the end of the depolarizing steps to +40 mV and expressed as a percentage of control values. Note that the percentage of blocked K Ca and K ATP current components were not significantly different whatever the order of gambierol-induced channel inhibition was. In ( b , c ), data represent the mean ± SEM of 4 different experiments.
Techniques Used: Inhibition
Figure Legend Snippet: Action of gambierol on the voltage-dependence of K + current activation in rat fetal AMC cells. The current was measured at the end of 90 ms depolarizing steps from a holding potential of −70 mV, expressed as a percentage of its maximal value at +40 mV, and plotted as a function of membrane potential during depolarizing pulses, in the absence (AGI, in blue) and presence (in red) of 100 nM of gambierol. Data represent the mean ± SEM of 4 different experiments. The theoretical curves correspond to data point fits according to the Boltzmann equation, as described in Materials and Methods, with V 50% and k values of 14.25 mV and 8.07 mV −1 (R 2 = 0.9989), respectively, for AGI, and 8.93 mV and 10.17 mV − 1 (R 2 = 0.9997), respectively, in the presence of gambierol. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin.
Techniques Used: Activation Assay
2) Product Images from "Hypoxia activates ATP-dependent potassium channels in inspiratory neurones of neonatal mice"
Article Title: Hypoxia activates ATP-dependent potassium channels in inspiratory neurones of neonatal mice
Journal: The Journal of Physiology
Figure Legend Snippet: Pharmacological properties of K ATP channels with 75 pS conductance in Ca 2+ -free pipette solution Drugs were applied at the beginning of each recording and representative traces are shown at the times indicated. The dotted lines indicate the closed level and dashed lines indicate the open levels. A, time course of diazoxide action. Patch holding potential, 0 mV. B and C, two representative experiments of K ATP channel inhibition in inside-out patches by tolbutamide ( B ) and glibenclamide ( C ) at holding potentials of -25 and -40 mV, respectively. The data shown in B and C were obtained in patches that had not been subjected to any prior treatment.
Techniques Used: Transferring, Inhibition