glibenclamide  (Alomone Labs)


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    Alomone Labs glibenclamide
    Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM <t>glibenclamide</t> and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.
    Glibenclamide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glibenclamide/product/Alomone Labs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    glibenclamide - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells"

    Article Title: Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells

    Journal: Toxins

    doi: 10.3390/toxins14040254

    Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM glibenclamide and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.
    Figure Legend Snippet: Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM glibenclamide and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.

    Techniques Used: Concentration Assay, Activation Assay, Blocking Assay

    The contribution of various current components (K Ca , K ATP ) and the action of gambierol (100 nM) on total K + current of rat fetal AMC cells. The K + current was recorded using the perforated whole-cell voltage-clamp technique, during 90 ms depolarizing steps from a holding potential of −70 mV, under the conditions indicated in the figure ( A , B ). Note in ( A , B ) the different sequences of gambierol addition to the external medium. ( a ) Superimposed traces of outward K + currents recorded under control conditions and after the perfusion of the different pharmacological agents indicated. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin. ( b ) Current-voltage relationships. The current was measured at the end of the depolarizing steps and expressed as a percentage of control values for the indicated agents. ( c ) Relative outward K + current contribution (expressed as a percentage of the total current) for the pharmacological treatments indicated. The K + current was measured at the end of the depolarizing steps to +40 mV and expressed as a percentage of control values. Note that the percentage of blocked K Ca and K ATP current components were not significantly different whatever the order of gambierol-induced channel inhibition was. In ( b , c ), data represent the mean ± SEM of 4 different experiments.
    Figure Legend Snippet: The contribution of various current components (K Ca , K ATP ) and the action of gambierol (100 nM) on total K + current of rat fetal AMC cells. The K + current was recorded using the perforated whole-cell voltage-clamp technique, during 90 ms depolarizing steps from a holding potential of −70 mV, under the conditions indicated in the figure ( A , B ). Note in ( A , B ) the different sequences of gambierol addition to the external medium. ( a ) Superimposed traces of outward K + currents recorded under control conditions and after the perfusion of the different pharmacological agents indicated. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin. ( b ) Current-voltage relationships. The current was measured at the end of the depolarizing steps and expressed as a percentage of control values for the indicated agents. ( c ) Relative outward K + current contribution (expressed as a percentage of the total current) for the pharmacological treatments indicated. The K + current was measured at the end of the depolarizing steps to +40 mV and expressed as a percentage of control values. Note that the percentage of blocked K Ca and K ATP current components were not significantly different whatever the order of gambierol-induced channel inhibition was. In ( b , c ), data represent the mean ± SEM of 4 different experiments.

    Techniques Used: Inhibition

    Action of gambierol on the voltage-dependence of K + current activation in rat fetal AMC cells. The current was measured at the end of 90 ms depolarizing steps from a holding potential of −70 mV, expressed as a percentage of its maximal value at +40 mV, and plotted as a function of membrane potential during depolarizing pulses, in the absence (AGI, in blue) and presence (in red) of 100 nM of gambierol. Data represent the mean ± SEM of 4 different experiments. The theoretical curves correspond to data point fits according to the Boltzmann equation, as described in Materials and Methods, with V 50% and k values of 14.25 mV and 8.07 mV −1 (R 2 = 0.9989), respectively, for AGI, and 8.93 mV and 10.17 mV − 1 (R 2 = 0.9997), respectively, in the presence of gambierol. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin.
    Figure Legend Snippet: Action of gambierol on the voltage-dependence of K + current activation in rat fetal AMC cells. The current was measured at the end of 90 ms depolarizing steps from a holding potential of −70 mV, expressed as a percentage of its maximal value at +40 mV, and plotted as a function of membrane potential during depolarizing pulses, in the absence (AGI, in blue) and presence (in red) of 100 nM of gambierol. Data represent the mean ± SEM of 4 different experiments. The theoretical curves correspond to data point fits according to the Boltzmann equation, as described in Materials and Methods, with V 50% and k values of 14.25 mV and 8.07 mV −1 (R 2 = 0.9989), respectively, for AGI, and 8.93 mV and 10.17 mV − 1 (R 2 = 0.9997), respectively, in the presence of gambierol. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin.

    Techniques Used: Activation Assay

    2) Product Images from "Hypoxia activates ATP-dependent potassium channels in inspiratory neurones of neonatal mice"

    Article Title: Hypoxia activates ATP-dependent potassium channels in inspiratory neurones of neonatal mice

    Journal: The Journal of Physiology

    doi: 10.1111/j.1469-7793.1998.755bm.x

    Pharmacological properties of K ATP channels with 75 pS conductance in Ca 2+ -free pipette solution Drugs were applied at the beginning of each recording and representative traces are shown at the times indicated. The dotted lines indicate the closed level and dashed lines indicate the open levels. A, time course of diazoxide action. Patch holding potential, 0 mV. B and C, two representative experiments of K ATP channel inhibition in inside-out patches by tolbutamide ( B ) and glibenclamide ( C ) at holding potentials of -25 and -40 mV, respectively. The data shown in B and C were obtained in patches that had not been subjected to any prior treatment.
    Figure Legend Snippet: Pharmacological properties of K ATP channels with 75 pS conductance in Ca 2+ -free pipette solution Drugs were applied at the beginning of each recording and representative traces are shown at the times indicated. The dotted lines indicate the closed level and dashed lines indicate the open levels. A, time course of diazoxide action. Patch holding potential, 0 mV. B and C, two representative experiments of K ATP channel inhibition in inside-out patches by tolbutamide ( B ) and glibenclamide ( C ) at holding potentials of -25 and -40 mV, respectively. The data shown in B and C were obtained in patches that had not been subjected to any prior treatment.

    Techniques Used: Transferring, Inhibition

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    Alomone Labs glibenclamide
    Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM <t>glibenclamide</t> and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.
    Glibenclamide, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glibenclamide/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glibenclamide - by Bioz Stars, 2022-08
    94/100 stars
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    Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM glibenclamide and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.

    Journal: Toxins

    Article Title: Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells

    doi: 10.3390/toxins14040254

    Figure Lengend Snippet: Time-course, kinetics and concentration-dependent action of gambierol on outward K + current in rat fetal AMC cells. ( A ) Time course of 20 nM gambierol action on the K + current measured at the end of 90 ms depolarizing pulses to +40 mV from a holding potential of −70 mV, applied every 10 s (see schema in ( B )). The red arrow indicates the time of polyether addition to the bath. ( B ) Superimposed K + current recorded every 10-s pulsing, before and after the perfusion of gambierol (20 nM), during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema). ( C ) Averaged normalized K + current recorded during 90 ms depolarizing steps to +40 mV from a holding potential of −70 mV (schema), under control conditions (black tracing), and after 20 nM gambierol (red tracing), note the slowing of the K + current activation. Data obtained from the same cell. The arrows indicate the activation time constants. ( D ) Concentration-response curve for the effect of gambierol on the steady-state K + current, measured after 90 ms depolarization steps to +40 mV from −70 mV. Each value, determined in the presence of 0.1–100 nM gambierol and normalized to its control value, represents the mean ± SEM of data obtained from 3–4 experiments. The theoretical curve was calculated as described in the text, with I SS , IC 50 and n H values of 42%, 5.8 nM and 0.89 (r 2 = 0.982), respectively. The external medium in A–D contained 1 µM TTX, 200 µM glibenclamide and 1 mM Cd 2+ to block, respectively, the Na V , K ATP and Ca V channels and prevent, indirectly, the activation of K Ca channels.

    Article Snippet: Tetrodotoxin, apamin, iberiotoxin, and glibenclamide were purchased from Alomone Labs (Jerusalem, Israel), and Latoxan (Portes-lès-Valence, France).

    Techniques: Concentration Assay, Activation Assay, Blocking Assay

    The contribution of various current components (K Ca , K ATP ) and the action of gambierol (100 nM) on total K + current of rat fetal AMC cells. The K + current was recorded using the perforated whole-cell voltage-clamp technique, during 90 ms depolarizing steps from a holding potential of −70 mV, under the conditions indicated in the figure ( A , B ). Note in ( A , B ) the different sequences of gambierol addition to the external medium. ( a ) Superimposed traces of outward K + currents recorded under control conditions and after the perfusion of the different pharmacological agents indicated. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin. ( b ) Current-voltage relationships. The current was measured at the end of the depolarizing steps and expressed as a percentage of control values for the indicated agents. ( c ) Relative outward K + current contribution (expressed as a percentage of the total current) for the pharmacological treatments indicated. The K + current was measured at the end of the depolarizing steps to +40 mV and expressed as a percentage of control values. Note that the percentage of blocked K Ca and K ATP current components were not significantly different whatever the order of gambierol-induced channel inhibition was. In ( b , c ), data represent the mean ± SEM of 4 different experiments.

    Journal: Toxins

    Article Title: Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells

    doi: 10.3390/toxins14040254

    Figure Lengend Snippet: The contribution of various current components (K Ca , K ATP ) and the action of gambierol (100 nM) on total K + current of rat fetal AMC cells. The K + current was recorded using the perforated whole-cell voltage-clamp technique, during 90 ms depolarizing steps from a holding potential of −70 mV, under the conditions indicated in the figure ( A , B ). Note in ( A , B ) the different sequences of gambierol addition to the external medium. ( a ) Superimposed traces of outward K + currents recorded under control conditions and after the perfusion of the different pharmacological agents indicated. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin. ( b ) Current-voltage relationships. The current was measured at the end of the depolarizing steps and expressed as a percentage of control values for the indicated agents. ( c ) Relative outward K + current contribution (expressed as a percentage of the total current) for the pharmacological treatments indicated. The K + current was measured at the end of the depolarizing steps to +40 mV and expressed as a percentage of control values. Note that the percentage of blocked K Ca and K ATP current components were not significantly different whatever the order of gambierol-induced channel inhibition was. In ( b , c ), data represent the mean ± SEM of 4 different experiments.

    Article Snippet: Tetrodotoxin, apamin, iberiotoxin, and glibenclamide were purchased from Alomone Labs (Jerusalem, Israel), and Latoxan (Portes-lès-Valence, France).

    Techniques: Inhibition

    Action of gambierol on the voltage-dependence of K + current activation in rat fetal AMC cells. The current was measured at the end of 90 ms depolarizing steps from a holding potential of −70 mV, expressed as a percentage of its maximal value at +40 mV, and plotted as a function of membrane potential during depolarizing pulses, in the absence (AGI, in blue) and presence (in red) of 100 nM of gambierol. Data represent the mean ± SEM of 4 different experiments. The theoretical curves correspond to data point fits according to the Boltzmann equation, as described in Materials and Methods, with V 50% and k values of 14.25 mV and 8.07 mV −1 (R 2 = 0.9989), respectively, for AGI, and 8.93 mV and 10.17 mV − 1 (R 2 = 0.9997), respectively, in the presence of gambierol. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin.

    Journal: Toxins

    Article Title: Gambierol Blocks a K+ Current Fraction without Affecting Catecholamine Release in Rat Fetal Adrenomedullary Cultured Chromaffin Cells

    doi: 10.3390/toxins14040254

    Figure Lengend Snippet: Action of gambierol on the voltage-dependence of K + current activation in rat fetal AMC cells. The current was measured at the end of 90 ms depolarizing steps from a holding potential of −70 mV, expressed as a percentage of its maximal value at +40 mV, and plotted as a function of membrane potential during depolarizing pulses, in the absence (AGI, in blue) and presence (in red) of 100 nM of gambierol. Data represent the mean ± SEM of 4 different experiments. The theoretical curves correspond to data point fits according to the Boltzmann equation, as described in Materials and Methods, with V 50% and k values of 14.25 mV and 8.07 mV −1 (R 2 = 0.9989), respectively, for AGI, and 8.93 mV and 10.17 mV − 1 (R 2 = 0.9997), respectively, in the presence of gambierol. AGI denotes the simultaneous perfusion of 400 nM apamin, 200 µM glibenclamide and 100 nM iberiotoxin.

    Article Snippet: Tetrodotoxin, apamin, iberiotoxin, and glibenclamide were purchased from Alomone Labs (Jerusalem, Israel), and Latoxan (Portes-lès-Valence, France).

    Techniques: Activation Assay

    (a) Representative traces show macroscopic K + currents (in black) after bath application of the Kir6.1 channel inhibitor glibenclamide (10 μM) (in red) and glibenclamide‐sensitive Kir6.1 currents (in gray) under 15 mM [K + ] o in a whole‐cell patched pericyte in NG2‐DsRed transgenic mouse hippocampus. (b) Representative traces show macroscopic K + currents (in black), macroscopic currents after bath application of the Kir4.1 channel antagonist VU0134992 (15 μM) (in red) and VU‐sensitive Kir4.1 currents (in purple) under 15 mM [K + ] o in a whole‐cell patched pericyte in NG2‐DsRed transgenic mouse hippocampus. (c) Left panel shows the average I/V plot of the Kir6.1 channel‐mediated currents and Kir4.1 channel‐mediated currents under 15 mM [K + ] o . Right panel shows the bar graph summary of the Kir6.1 current and Kir4.1 current when the cell's voltage was held at −142 mV under 15 mM [K + ] o . n = 6 for each group. The error bars represent SEM. ** indicates p

    Journal: Glia

    Article Title: Homogeneity or heterogeneity, the paradox of neurovascular pericytes in the brain). Homogeneity or heterogeneity, the paradox of neurovascular pericytes in the brain

    doi: 10.1002/glia.24054

    Figure Lengend Snippet: (a) Representative traces show macroscopic K + currents (in black) after bath application of the Kir6.1 channel inhibitor glibenclamide (10 μM) (in red) and glibenclamide‐sensitive Kir6.1 currents (in gray) under 15 mM [K + ] o in a whole‐cell patched pericyte in NG2‐DsRed transgenic mouse hippocampus. (b) Representative traces show macroscopic K + currents (in black), macroscopic currents after bath application of the Kir4.1 channel antagonist VU0134992 (15 μM) (in red) and VU‐sensitive Kir4.1 currents (in purple) under 15 mM [K + ] o in a whole‐cell patched pericyte in NG2‐DsRed transgenic mouse hippocampus. (c) Left panel shows the average I/V plot of the Kir6.1 channel‐mediated currents and Kir4.1 channel‐mediated currents under 15 mM [K + ] o . Right panel shows the bar graph summary of the Kir6.1 current and Kir4.1 current when the cell's voltage was held at −142 mV under 15 mM [K + ] o . n = 6 for each group. The error bars represent SEM. ** indicates p

    Article Snippet: Kir6.1 channel bloker glibenclamide was purchased from Alomone lab and the working concentration was 10 μM.

    Techniques: Transgenic Assay

    Pharmacological properties of K ATP channels with 75 pS conductance in Ca 2+ -free pipette solution Drugs were applied at the beginning of each recording and representative traces are shown at the times indicated. The dotted lines indicate the closed level and dashed lines indicate the open levels. A, time course of diazoxide action. Patch holding potential, 0 mV. B and C, two representative experiments of K ATP channel inhibition in inside-out patches by tolbutamide ( B ) and glibenclamide ( C ) at holding potentials of -25 and -40 mV, respectively. The data shown in B and C were obtained in patches that had not been subjected to any prior treatment.

    Journal: The Journal of Physiology

    Article Title: Hypoxia activates ATP-dependent potassium channels in inspiratory neurones of neonatal mice

    doi: 10.1111/j.1469-7793.1998.755bm.x

    Figure Lengend Snippet: Pharmacological properties of K ATP channels with 75 pS conductance in Ca 2+ -free pipette solution Drugs were applied at the beginning of each recording and representative traces are shown at the times indicated. The dotted lines indicate the closed level and dashed lines indicate the open levels. A, time course of diazoxide action. Patch holding potential, 0 mV. B and C, two representative experiments of K ATP channel inhibition in inside-out patches by tolbutamide ( B ) and glibenclamide ( C ) at holding potentials of -25 and -40 mV, respectively. The data shown in B and C were obtained in patches that had not been subjected to any prior treatment.

    Article Snippet: TTX and K+ channel blockers (tetraethylammonium (TEA), 4-aminopyridine, charybdotoxin, iberiotoxin, diazoxide, tolbutamide and glibenclamide) were purchased from Alomone Labs (Israel).

    Techniques: Transferring, Inhibition