Structured Review

Millipore girk2
Immunohistological analysis of hVM tissue at 20 weeks post-graft. Immunohistochemistry of TH + ve neurons (brown) and HuNu (blue) in the hVM graft (top panel). From left to right, images depict representative tissue from a Control rat (A), Lesion rat (B) and a large hVM graft (C). The central panel depicts A9 TH + ve neurons (green) co-labelled with <t>Girk2</t> (red, D); A10 TH + ve neurons (green) co-labelled with Calbindin (red, E); × 10 magnification of hVM cells with TH + ve neurons stained in brown and HuNu + ve cells in blue (F). The bottom panel depicts the number of TH + ve cells per individual graft (G) and as a group mean (H), as well as the proportion of girk2 + ve (I) and calbindin + ve (J) cells out of the total number of TH + ve neurons. Scale bar = 1000 μm. Error bars = ± standard error of mean. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Girk2, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 17 article reviews
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girk2 - by Bioz Stars, 2022-11
93/100 stars

Images

1) Product Images from "Amelioration of non-motor dysfunctions after transplantation of human dopamine neurons in a model of Parkinson's disease"

Article Title: Amelioration of non-motor dysfunctions after transplantation of human dopamine neurons in a model of Parkinson's disease

Journal: Experimental Neurology

doi: 10.1016/j.expneurol.2016.02.003

Immunohistological analysis of hVM tissue at 20 weeks post-graft. Immunohistochemistry of TH + ve neurons (brown) and HuNu (blue) in the hVM graft (top panel). From left to right, images depict representative tissue from a Control rat (A), Lesion rat (B) and a large hVM graft (C). The central panel depicts A9 TH + ve neurons (green) co-labelled with Girk2 (red, D); A10 TH + ve neurons (green) co-labelled with Calbindin (red, E); × 10 magnification of hVM cells with TH + ve neurons stained in brown and HuNu + ve cells in blue (F). The bottom panel depicts the number of TH + ve cells per individual graft (G) and as a group mean (H), as well as the proportion of girk2 + ve (I) and calbindin + ve (J) cells out of the total number of TH + ve neurons. Scale bar = 1000 μm. Error bars = ± standard error of mean. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Figure Legend Snippet: Immunohistological analysis of hVM tissue at 20 weeks post-graft. Immunohistochemistry of TH + ve neurons (brown) and HuNu (blue) in the hVM graft (top panel). From left to right, images depict representative tissue from a Control rat (A), Lesion rat (B) and a large hVM graft (C). The central panel depicts A9 TH + ve neurons (green) co-labelled with Girk2 (red, D); A10 TH + ve neurons (green) co-labelled with Calbindin (red, E); × 10 magnification of hVM cells with TH + ve neurons stained in brown and HuNu + ve cells in blue (F). The bottom panel depicts the number of TH + ve cells per individual graft (G) and as a group mean (H), as well as the proportion of girk2 + ve (I) and calbindin + ve (J) cells out of the total number of TH + ve neurons. Scale bar = 1000 μm. Error bars = ± standard error of mean. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Techniques Used: Immunohistochemistry, Staining

2) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

3) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

4) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

5) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

6) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

7) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

8) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

9) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

10) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

11) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

12) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

13) Product Images from "Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels"

Article Title: Suprachiasmatic nucleus function and circadian entrainment are modulated by G protein-coupled inwardly rectifying (GIRK) channels

Journal: The Journal of Physiology

doi: 10.1113/jphysiol.2014.282079

GIRK2 is necessary for NPY-induced phase shifts in the molecular clock
Figure Legend Snippet: GIRK2 is necessary for NPY-induced phase shifts in the molecular clock

Techniques Used:

Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate
Figure Legend Snippet: Loss of GIRK2 reduces effect of NPY on SCN neuron spontaneous firing rate

Techniques Used:

GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance
Figure Legend Snippet: GIRK2 knockout mice entrain more rapidly to a 6 h light cycle advance

Techniques Used: Knock-Out, Mouse Assay

GIRK2, but not GIRK1, protein levels are regulated in a circadian manner
Figure Legend Snippet: GIRK2, but not GIRK1, protein levels are regulated in a circadian manner

Techniques Used:

GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity
Figure Legend Snippet: GIRK2 knockout mice fail to shorten free-running period in response to wheel-running activity

Techniques Used: Knock-Out, Mouse Assay, Activity Assay

GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls
Figure Legend Snippet: GIRK2 knockout SCN neurons exhibit more depolarized resting membrane potential compared to wild-type controls

Techniques Used: Knock-Out

Loss of GIRK2 alters the behavioural response to non-photic and photic cues
Figure Legend Snippet: Loss of GIRK2 alters the behavioural response to non-photic and photic cues

Techniques Used:

14) Product Images from "Xeno-Free Defined Conditions for Culture of Human Embryonic Stem Cells, Neural Stem Cells and Dopaminergic Neurons Derived from Them"

Article Title: Xeno-Free Defined Conditions for Culture of Human Embryonic Stem Cells, Neural Stem Cells and Dopaminergic Neurons Derived from Them

Journal: PLoS ONE

doi: 10.1371/journal.pone.0006233

NSCs from prolonged culture in defined conditions can be efficiently differentiated into midbrain dopaminergic neurons. (A–E) Efficient differentiation into midbrain dopaminergic neurons by PA6-CM as shown by immunocytochemistry. The majority of the cells expressed β-III-tubulin and TH after 4 weeks of differentiation in PA6-CM (A–B). Co-expression of midbrain and A9 markers in TH + dopaminergic neurons: Lmx1a (C), VMAT (D) and Girk2 (E). (F) Differential expression of dopaminergic markers in several stages of differentiation (NSC, dopaminergic precursors and dopaminergic neurons) by quantitative PCR. All the examined markers were up-regulated in dopaminergic populations compared to NSCs.
Figure Legend Snippet: NSCs from prolonged culture in defined conditions can be efficiently differentiated into midbrain dopaminergic neurons. (A–E) Efficient differentiation into midbrain dopaminergic neurons by PA6-CM as shown by immunocytochemistry. The majority of the cells expressed β-III-tubulin and TH after 4 weeks of differentiation in PA6-CM (A–B). Co-expression of midbrain and A9 markers in TH + dopaminergic neurons: Lmx1a (C), VMAT (D) and Girk2 (E). (F) Differential expression of dopaminergic markers in several stages of differentiation (NSC, dopaminergic precursors and dopaminergic neurons) by quantitative PCR. All the examined markers were up-regulated in dopaminergic populations compared to NSCs.

Techniques Used: Immunocytochemistry, Expressing, Real-time Polymerase Chain Reaction

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  • 90
    Millipore k channel subunit 2
    Laminin-based SAP hydrogels bias A9 DA specification. ( A , B ) Representative photomicrographs illustrating the subpopulations of DA neurons depicted by GFP+ co-expression with or without <t>GIRK2</t> and/or Calbinidn (CALB+) within a ( A ) Cells or ( B ) Cells + SAP graft, at 12 weeks. ( Ai , Bi ) High power images from ( A , B ). ( C ) Quantitative assessment of GFP+ DA neurons co-expressing GIRK2+, CALB+ and GIRK + CALB+, noting SAP scaffolds promoted GIRK2+ DA neuron maturation. ( D ) Expressed as a proportion of GFP+ DA cells, only grafts in the presence of the tissue-specific IKVAV SAP hydrogel showed a significant increase in the percentage of TH+/GIRK2+ (A9-like DA neurons). Data represent Mean ± SEM, n = 5–7 Grafts/group. * p
    K Channel Subunit 2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k channel subunit 2/product/Millipore
    Average 90 stars, based on 1 article reviews
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    k channel subunit 2 - by Bioz Stars, 2022-11
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    88
    Millipore rabbit anti girk2
    Identification of dopamine neuron subtypes within the intranigral and intrastriatal transplants four months post-transplantation. High magnification confocal photomicrographs of coronal sections showing GFP (GFP; green), calbindin (Calb; blue), and <t>Girk2</t> (Girk; red) in either intranigral ( a – e ) or intrastriatal ( f – j ) transplants. The GFP+/calbindin+ double-labelled neurons (red arrows) were located preferentially towards the center of both the intranigral ( a , b ) and intrastriatal ( f , g ) grafts. These cells were of the typical small and rounded morphology indicative of the A10 dopamine neuron types ( i , j ). The GFP+/Girk2+ double-labelled neurons (white arrows) were located preferentially in the periphery of both the intranigral ( a , c ) and intrastriatal ( f , h ) grafts and were of the typical large and angular morphology ( d , e ) indicative of the A9 dopamine neuron type. Yellow arrows point to rare populations of GFP+/calbindin+/Girk2+ cells. Also shown are quantifications of the number of surviving GFP+/Girk2+ ( k ) or GFP+/calbindin+ ( l ) cells within intrastriatal ( n = 7) and intranigral ( n = 6) transplants and the percentage of GFP+/calbindin+ ( m ) or GFP+/Girk2+ ( n ) cells within intrastriatal and intranigral transplants. Note that, although there are more surviving GFP+/Girk2+ and GFP+/calbindin+ in intranigral versus intrastriatal transplants, the proportion of these dopaminergic neurons was similar in both transplantation conditions. Data are presented as mean ± SEM. Comparison between the groups was performed by a Mann–Whitney nonparametric test. * p
    Rabbit Anti Girk2, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti girk2/product/Millipore
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    92
    Millipore anti potassium channel kir3 2 girk2 antibody
    Identification of dopamine neuron subtypes within the intranigral and intrastriatal transplants four months post-transplantation. High magnification confocal photomicrographs of coronal sections showing GFP (GFP; green), calbindin (Calb; blue), and <t>Girk2</t> (Girk; red) in either intranigral ( a – e ) or intrastriatal ( f – j ) transplants. The GFP+/calbindin+ double-labelled neurons (red arrows) were located preferentially towards the center of both the intranigral ( a , b ) and intrastriatal ( f , g ) grafts. These cells were of the typical small and rounded morphology indicative of the A10 dopamine neuron types ( i , j ). The GFP+/Girk2+ double-labelled neurons (white arrows) were located preferentially in the periphery of both the intranigral ( a , c ) and intrastriatal ( f , h ) grafts and were of the typical large and angular morphology ( d , e ) indicative of the A9 dopamine neuron type. Yellow arrows point to rare populations of GFP+/calbindin+/Girk2+ cells. Also shown are quantifications of the number of surviving GFP+/Girk2+ ( k ) or GFP+/calbindin+ ( l ) cells within intrastriatal ( n = 7) and intranigral ( n = 6) transplants and the percentage of GFP+/calbindin+ ( m ) or GFP+/Girk2+ ( n ) cells within intrastriatal and intranigral transplants. Note that, although there are more surviving GFP+/Girk2+ and GFP+/calbindin+ in intranigral versus intrastriatal transplants, the proportion of these dopaminergic neurons was similar in both transplantation conditions. Data are presented as mean ± SEM. Comparison between the groups was performed by a Mann–Whitney nonparametric test. * p
    Anti Potassium Channel Kir3 2 Girk2 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti potassium channel kir3 2 girk2 antibody/product/Millipore
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    Image Search Results


    Laminin-based SAP hydrogels bias A9 DA specification. ( A , B ) Representative photomicrographs illustrating the subpopulations of DA neurons depicted by GFP+ co-expression with or without GIRK2 and/or Calbinidn (CALB+) within a ( A ) Cells or ( B ) Cells + SAP graft, at 12 weeks. ( Ai , Bi ) High power images from ( A , B ). ( C ) Quantitative assessment of GFP+ DA neurons co-expressing GIRK2+, CALB+ and GIRK + CALB+, noting SAP scaffolds promoted GIRK2+ DA neuron maturation. ( D ) Expressed as a proportion of GFP+ DA cells, only grafts in the presence of the tissue-specific IKVAV SAP hydrogel showed a significant increase in the percentage of TH+/GIRK2+ (A9-like DA neurons). Data represent Mean ± SEM, n = 5–7 Grafts/group. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Extracellular Matrix Biomimetic Hydrogels, Encapsulated with Stromal Cell-Derived Factor 1, Improve the Composition of Foetal Tissue Grafts in a Rodent Model of Parkinson’s Disease

    doi: 10.3390/ijms23094646

    Figure Lengend Snippet: Laminin-based SAP hydrogels bias A9 DA specification. ( A , B ) Representative photomicrographs illustrating the subpopulations of DA neurons depicted by GFP+ co-expression with or without GIRK2 and/or Calbinidn (CALB+) within a ( A ) Cells or ( B ) Cells + SAP graft, at 12 weeks. ( Ai , Bi ) High power images from ( A , B ). ( C ) Quantitative assessment of GFP+ DA neurons co-expressing GIRK2+, CALB+ and GIRK + CALB+, noting SAP scaffolds promoted GIRK2+ DA neuron maturation. ( D ) Expressed as a proportion of GFP+ DA cells, only grafts in the presence of the tissue-specific IKVAV SAP hydrogel showed a significant increase in the percentage of TH+/GIRK2+ (A9-like DA neurons). Data represent Mean ± SEM, n = 5–7 Grafts/group. * p

    Article Snippet: Primary antibodies and dilution factors were as follows: mouse anti-Calbindin (1:1,500: Swant), rabbit anti-cleaved caspase-3 (Asp175, 1:100; Cell Signalling Technology, Danvers, MA, USA), rabbit anti-GFAP (1:200, DAKO, Santa Clara, CA, USA), mouse anti-CD11b (1:100, Serotec, Oxford, UK), rabbit anti- G-protein-gated inwardly rectifying K+ channel subunit 2 (GIRK2, 1:500; Chemicon, Temecula, CA, USA), chicken anti-GFP (1:1,000; Abcam, Cambridge UK), rabbit anti-GFP (1:20,000; Abcam, Cambridge UK), rabbit anti-KI67 (1:1000, Novocastra, Sheffiled UK), mouse anti-NeuN (1:100; R & D Systems), rabbit anti-TH (1:500, Pel-Freeze), sheep anti-TH (1:800, Pelfreez, Rogers, AR, USA).

    Techniques: Expressing

    Identification of dopamine neuron subtypes within the intranigral and intrastriatal transplants four months post-transplantation. High magnification confocal photomicrographs of coronal sections showing GFP (GFP; green), calbindin (Calb; blue), and Girk2 (Girk; red) in either intranigral ( a – e ) or intrastriatal ( f – j ) transplants. The GFP+/calbindin+ double-labelled neurons (red arrows) were located preferentially towards the center of both the intranigral ( a , b ) and intrastriatal ( f , g ) grafts. These cells were of the typical small and rounded morphology indicative of the A10 dopamine neuron types ( i , j ). The GFP+/Girk2+ double-labelled neurons (white arrows) were located preferentially in the periphery of both the intranigral ( a , c ) and intrastriatal ( f , h ) grafts and were of the typical large and angular morphology ( d , e ) indicative of the A9 dopamine neuron type. Yellow arrows point to rare populations of GFP+/calbindin+/Girk2+ cells. Also shown are quantifications of the number of surviving GFP+/Girk2+ ( k ) or GFP+/calbindin+ ( l ) cells within intrastriatal ( n = 7) and intranigral ( n = 6) transplants and the percentage of GFP+/calbindin+ ( m ) or GFP+/Girk2+ ( n ) cells within intrastriatal and intranigral transplants. Note that, although there are more surviving GFP+/Girk2+ and GFP+/calbindin+ in intranigral versus intrastriatal transplants, the proportion of these dopaminergic neurons was similar in both transplantation conditions. Data are presented as mean ± SEM. Comparison between the groups was performed by a Mann–Whitney nonparametric test. * p

    Journal: Cells

    Article Title: Better Outcomes with Intranigral versus Intrastriatal Cell Transplantation: Relevance for Parkinson’s Disease

    doi: 10.3390/cells11071191

    Figure Lengend Snippet: Identification of dopamine neuron subtypes within the intranigral and intrastriatal transplants four months post-transplantation. High magnification confocal photomicrographs of coronal sections showing GFP (GFP; green), calbindin (Calb; blue), and Girk2 (Girk; red) in either intranigral ( a – e ) or intrastriatal ( f – j ) transplants. The GFP+/calbindin+ double-labelled neurons (red arrows) were located preferentially towards the center of both the intranigral ( a , b ) and intrastriatal ( f , g ) grafts. These cells were of the typical small and rounded morphology indicative of the A10 dopamine neuron types ( i , j ). The GFP+/Girk2+ double-labelled neurons (white arrows) were located preferentially in the periphery of both the intranigral ( a , c ) and intrastriatal ( f , h ) grafts and were of the typical large and angular morphology ( d , e ) indicative of the A9 dopamine neuron type. Yellow arrows point to rare populations of GFP+/calbindin+/Girk2+ cells. Also shown are quantifications of the number of surviving GFP+/Girk2+ ( k ) or GFP+/calbindin+ ( l ) cells within intrastriatal ( n = 7) and intranigral ( n = 6) transplants and the percentage of GFP+/calbindin+ ( m ) or GFP+/Girk2+ ( n ) cells within intrastriatal and intranigral transplants. Note that, although there are more surviving GFP+/Girk2+ and GFP+/calbindin+ in intranigral versus intrastriatal transplants, the proportion of these dopaminergic neurons was similar in both transplantation conditions. Data are presented as mean ± SEM. Comparison between the groups was performed by a Mann–Whitney nonparametric test. * p

    Article Snippet: The following antibodies were used in this study: goat anti-GFP (1/1000; Abcam, Cambridge, UK), rabbit anti-GFP (1:1000; Life Technologies), chicken anti-GFP (1:1000, Abcam), mouse anti-TH (1:5000; ImmunoStar, Hudson, WI, USA), rabbit anti-Girk2 (1:100; Sigma-Aldrich), rabbit anti-calbindin (1:5000; Swant, Burgdorf, Switzerland), rabbit anti-DAT (1:5000; a generous gift from B. Bloch, CNRS UMR 5227 Bordeaux-France), mouse anti-NeuN (1:500; Sigma-Aldrich), chicken anti-GFAP (1:1000; Abcam), and rabbit anti-Iba1 (1:500; Fujifilm Wako, Osaka, Japan) Rabbit anti-CD86 (1:200; Abcam) and goat anti-Arg1 (1:250; Santa Cruz Biotechnology, Dallas, TX, USA.) were used for neurotoxic and neuroprotective phenotypes, respectively.

    Techniques: Transplantation Assay, MANN-WHITNEY