gibson assembly kit  (New England Biolabs)


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    Structured Review

    New England Biolabs gibson assembly kit
    Gibson Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gibson assembly kit/product/New England Biolabs
    Average 99 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    gibson assembly kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: .. PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment. ..

    Amplification:

    Article Title: A Ubiquitous Platform for Bacterial Nanotube Biogenesis
    Article Snippet: SH203 (ΔfliO::tet, Pfla/che -fliP-cheD, sacA:: Pfla/che -fliO -spec ) was constructed by transforming SH103 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliO comp1-2 and Comp PflgB 1-R. SH204 (ΔfliP::tet, Pfla/che -fliQ-cheD, sacA:: Pfla/che -fliP -spec ) was constructed by transforming SH104 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliP comp1-2 and Comp PflgB 1-R. SH205 (ΔfliQ::tet, Pfla/che -fliR-cheD, sacA:: Pfla/che -fliQ -spec ) was constructed by transforming SH105 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliQ comp1-2 and Comp PflgB 1-R. SH206 (ΔfliR::tet, Pfla/che -flhB-cheD, sacA:: Pfla/che -fliR -spec ) was constructed by transforming SH106 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliR comp1-2 and Comp PflgB 1-R. SH207 (ΔflhB::tet, Pfla/che -flhA-cheD, sacA:: Pfla/che -flhB -spec ) was constructed by transforming SH107 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, flhB comp1-2 and Comp PflgB 1-R. SH208 (ΔflhA::tet, Pfla/che -flhF-cheD, sacA:: Pfla/che -flhA -spec ) was constructed by transforming SH108 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, flhA comp1-2 and Comp PflgB 1-R. SH209 [ΔfliO-flhA::tet , sacA:: Pfla/che -CORE -F Lm (fliPQRflhBA )-spec ] was constructed by transforming SH9 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, Lm CORE comp1-2 and Comp PflgB 1-R. .. CORE -F Lm (fliPQRflhBA ) was amplified from Lm (10403S) gDNA using primers Lm CORE comp 1-2.

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: Plasmids construction and in vitro transcription pT7T-EcadTSMod, pT7T-EcadTL, pT7T-EcadTI, pT7T-CcadTSMod were obtained by PCR amplification of EcadTSMod, EcadTL and EcadTI plasmids (a Kind gift of Dr. N. Borghi) and C-cadherin cDNA (kindly provided by Dr. BM Gumbiner), respectively. .. PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment.

    In Vitro:

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: Paragraph title: Plasmids construction and in vitro transcription ... PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment.

    Construct:

    Article Title: A Ubiquitous Platform for Bacterial Nanotube Biogenesis
    Article Snippet: .. SH203 (ΔfliO::tet, Pfla/che -fliP-cheD, sacA:: Pfla/che -fliO -spec ) was constructed by transforming SH103 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliO comp1-2 and Comp PflgB 1-R. SH204 (ΔfliP::tet, Pfla/che -fliQ-cheD, sacA:: Pfla/che -fliP -spec ) was constructed by transforming SH104 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliP comp1-2 and Comp PflgB 1-R. SH205 (ΔfliQ::tet, Pfla/che -fliR-cheD, sacA:: Pfla/che -fliQ -spec ) was constructed by transforming SH105 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliQ comp1-2 and Comp PflgB 1-R. SH206 (ΔfliR::tet, Pfla/che -flhB-cheD, sacA:: Pfla/che -fliR -spec ) was constructed by transforming SH106 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliR comp1-2 and Comp PflgB 1-R. SH207 (ΔflhB::tet, Pfla/che -flhA-cheD, sacA:: Pfla/che -flhB -spec ) was constructed by transforming SH107 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, flhB comp1-2 and Comp PflgB 1-R. SH208 (ΔflhA::tet, Pfla/che -flhF-cheD, sacA:: Pfla/che -flhA -spec ) was constructed by transforming SH108 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, flhA comp1-2 and Comp PflgB 1-R. SH209 [ΔfliO-flhA::tet , sacA:: Pfla/che -CORE -F Lm (fliPQRflhBA )-spec ] was constructed by transforming SH9 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, Lm CORE comp1-2 and Comp PflgB 1-R. .. CORE -F Lm (fliPQRflhBA ) was amplified from Lm (10403S) gDNA using primers Lm CORE comp 1-2.

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: .. PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment. ..

    Polymerase Chain Reaction:

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: .. PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment. ..

    Generated:

    Article Title: A Ubiquitous Platform for Bacterial Nanotube Biogenesis
    Article Snippet: .. SH203 (ΔfliO::tet, Pfla/che -fliP-cheD, sacA:: Pfla/che -fliO -spec ) was constructed by transforming SH103 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliO comp1-2 and Comp PflgB 1-R. SH204 (ΔfliP::tet, Pfla/che -fliQ-cheD, sacA:: Pfla/che -fliP -spec ) was constructed by transforming SH104 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliP comp1-2 and Comp PflgB 1-R. SH205 (ΔfliQ::tet, Pfla/che -fliR-cheD, sacA:: Pfla/che -fliQ -spec ) was constructed by transforming SH105 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliQ comp1-2 and Comp PflgB 1-R. SH206 (ΔfliR::tet, Pfla/che -flhB-cheD, sacA:: Pfla/che -fliR -spec ) was constructed by transforming SH106 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, fliR comp1-2 and Comp PflgB 1-R. SH207 (ΔflhB::tet, Pfla/che -flhA-cheD, sacA:: Pfla/che -flhB -spec ) was constructed by transforming SH107 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, flhB comp1-2 and Comp PflgB 1-R. SH208 (ΔflhA::tet, Pfla/che -flhF-cheD, sacA:: Pfla/che -flhA -spec ) was constructed by transforming SH108 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, flhA comp1-2 and Comp PflgB 1-R. SH209 [ΔfliO-flhA::tet , sacA:: Pfla/che -CORE -F Lm (fliPQRflhBA )-spec ] was constructed by transforming SH9 with complementation construct generated using Gibson assembly kit (NEB, USA) utilizing primers sacA P1-P4, Lm CORE comp1-2 and Comp PflgB 1-R. .. CORE -F Lm (fliPQRflhBA ) was amplified from Lm (10403S) gDNA using primers Lm CORE comp 1-2.

    Sequencing:

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: .. PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment. ..

    Plasmid Preparation:

    Article Title: Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early Xenopus laevis embryos
    Article Snippet: .. PCR products were cloned between BglII and SpeI sites into the pT7T vector using the NEB Gibson assembly kit (NEB). pT7-CcadTSMod was constructed with the NEB Gibson assembly kit following the same scheme as for EcadTSMod: the sequence coding for the 734 first amino acid of C-cadherin were inserted after the BglII site of pT7T, the TS module containing the spider silk protein flanked by mTFP1, EYFP and glycine linker at each side, is inserted just after the first fragment. ..

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  • 99
    New England Biolabs gibson assembly kit
    Gibson Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gibson assembly kit/product/New England Biolabs
    Average 99 stars, based on 114 article reviews
    Price from $9.99 to $1999.99
    gibson assembly kit - by Bioz Stars, 2020-01
    99/100 stars
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