Structured Review

Affibody mtrx gfp
PET transaxial images ( a , b , the colour scales are the same), histograms ( c , d ) of the heterogeneity contributions (the mean intensity deviation per distance calculated according to Eq. 2 ) and surface plots ( e , f ) of the uptake of AnxA5 and <t>mTrx-GFP</t> in a FaDu xenograft. The imaging was performed in the same animal > 2 h apart on the same day. In e and f , the X - and Y -axes represent spatial dimensions and the Z -axis is the mean tracer uptake (SUV mean )
Mtrx Gfp, supplied by Affibody, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mtrx gfp/product/Affibody
Average 79 stars, based on 6 article reviews
Price from $9.99 to $1999.99
mtrx gfp - by Bioz Stars, 2020-02
79/100 stars

Images

1) Product Images from "A method for comparing intra-tumoural radioactivity uptake heterogeneity in preclinical positron emission tomography studies"

Article Title: A method for comparing intra-tumoural radioactivity uptake heterogeneity in preclinical positron emission tomography studies

Journal: EJNMMI Physics

doi: 10.1186/s40658-015-0124-1

PET transaxial images ( a , b , the colour scales are the same), histograms ( c , d ) of the heterogeneity contributions (the mean intensity deviation per distance calculated according to Eq. 2 ) and surface plots ( e , f ) of the uptake of AnxA5 and mTrx-GFP in a FaDu xenograft. The imaging was performed in the same animal > 2 h apart on the same day. In e and f , the X - and Y -axes represent spatial dimensions and the Z -axis is the mean tracer uptake (SUV mean )
Figure Legend Snippet: PET transaxial images ( a , b , the colour scales are the same), histograms ( c , d ) of the heterogeneity contributions (the mean intensity deviation per distance calculated according to Eq. 2 ) and surface plots ( e , f ) of the uptake of AnxA5 and mTrx-GFP in a FaDu xenograft. The imaging was performed in the same animal > 2 h apart on the same day. In e and f , the X - and Y -axes represent spatial dimensions and the Z -axis is the mean tracer uptake (SUV mean )

Techniques Used: Positron Emission Tomography, Imaging

2) Product Images from "Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors"

Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors

Journal: Journal of the American Chemical Society

doi: 10.1021/jacs.8b07601

(A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected 4T1 cells grown either with (right) or without (left) 15 μ g/mL cumate to induce EGFR and GFP expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.
Figure Legend Snippet: (A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected 4T1 cells grown either with (right) or without (left) 15 μ g/mL cumate to induce EGFR and GFP expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.

Techniques Used: Fluorescence, Microscopy, Transfection, Expressing, Western Blot, Imaging, Molecular Weight

(A) Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) showing photo-cross-linking products of either N23BP or WT to EGFR, with or without irradiation at 980 nm. Note that only 980 nm irradiation of N23BP produced a photoproduct significantly different than free EGFR (right lane). Ladder proteins are (top to bottom) 100, 75, and 50 kDa. (B) Retention of fluorescently labeled affibodies (left, N23BP; right, WT) in 3D tumor spheroids grown from transfected 4T1 cells either induced with 15 μ g/mL cumate. Concentrations of retained affibodies were calculated by comparing spheroid lysate fluorescence to standard curves prepared for each labeled affibody. “20 IR” designates that this sample was irradiated at 980 nm after 3 h incubation, then left to grow to a total of 20 h. Lanes without an IR designation were not irradiated.
Figure Legend Snippet: (A) Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) showing photo-cross-linking products of either N23BP or WT to EGFR, with or without irradiation at 980 nm. Note that only 980 nm irradiation of N23BP produced a photoproduct significantly different than free EGFR (right lane). Ladder proteins are (top to bottom) 100, 75, and 50 kDa. (B) Retention of fluorescently labeled affibodies (left, N23BP; right, WT) in 3D tumor spheroids grown from transfected 4T1 cells either induced with 15 μ g/mL cumate. Concentrations of retained affibodies were calculated by comparing spheroid lysate fluorescence to standard curves prepared for each labeled affibody. “20 IR” designates that this sample was irradiated at 980 nm after 3 h incubation, then left to grow to a total of 20 h. Lanes without an IR designation were not irradiated.

Techniques Used: Polyacrylamide Gel Electrophoresis, SDS Page, Irradiation, Produced, Labeling, Transfection, Fluorescence, Incubation

Left: Spheroids were treated with 1 μ M N23BP (top) or WT (bottom) affibody for a total of 4 h (left column) and 20 h (middle and right columns) and additionally irradiated with 365 nm light for 30 min after 3.5 h incubation (right column). These spheroids were sectioned at approximately the same depth and imaged for Rhodamine and GFP distribution. Micrographs show Rhodamine signal within each section normalized by the average GFP intensity. Scale bars are 200 μ m. Right: Five spheroids were grown and irradiated for 1 h with the indicated N23BP affibody concentration in growth media. Affibody containing media was removed, and spheroids were lysed and loaded onto a PAGE gel and probed for affibody conjugates using an anti-T7 antibody. High molecular weight bands around the expected molecular weight of EGFR were observed only when the spheroid-affibody mixtures were irradiated, indicating photo-cross-linking.
Figure Legend Snippet: Left: Spheroids were treated with 1 μ M N23BP (top) or WT (bottom) affibody for a total of 4 h (left column) and 20 h (middle and right columns) and additionally irradiated with 365 nm light for 30 min after 3.5 h incubation (right column). These spheroids were sectioned at approximately the same depth and imaged for Rhodamine and GFP distribution. Micrographs show Rhodamine signal within each section normalized by the average GFP intensity. Scale bars are 200 μ m. Right: Five spheroids were grown and irradiated for 1 h with the indicated N23BP affibody concentration in growth media. Affibody containing media was removed, and spheroids were lysed and loaded onto a PAGE gel and probed for affibody conjugates using an anti-T7 antibody. High molecular weight bands around the expected molecular weight of EGFR were observed only when the spheroid-affibody mixtures were irradiated, indicating photo-cross-linking.

Techniques Used: Irradiation, Incubation, Concentration Assay, Polyacrylamide Gel Electrophoresis, Molecular Weight

Related Articles

Conjugation Assay:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: High degree random conjugation may inevitably results in changes in antigen binding characteristics and, in more extreme scenario, a complete loss of function [ ]. .. IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs.

Selection:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: Paragraph title: 2. Selection of Antigen Binding Molecules ... IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs.

Amplification:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. .. For example, to obtain useful VHHs found in camelids ( B), a cDNA library can be obtained from an immunized llama or alpaca to serve as a pool of amplified DNA fragments for generation of the phage display library [ ].

In Vitro:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: .. IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. ..

Fluorescence:

Article Title: Fluorescent Affibody Peptide Penetration in Glioma Margin Is Superior to Full Antibody
Article Snippet: .. Results . shows the GFP outlined tumor, H & E staining of the same tissue section and raw fluorescence at both the Affibody and cetuximab channels. .. The most striking observation of the Affibody and cetuximab maps is that on average cetuximab appears to be more confined to tumor interiors , while the Affibody appears more evenly dispersed throughout the tumor ( ).

Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors
Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP. .. The fluorescence in the lysis buffer was measured using the plate reader at 572 nm, and affibody concentration was determined by comparison to a calibration curve.

Article Title: A method for comparing intra-tumoural radioactivity uptake heterogeneity in preclinical positron emission tomography studies
Article Snippet: .. For radioligands, the methyl-11 C-radiolabelled Annexin A5, [methyl-11 C]-His6 -AnxA5-ST-CH3 , hereafter denoted AnxA5 (~38 kDa), mutated-thioredoxin-green fluorescence protein [methyl-11 C]-His6 -mTrx-GFP-ST-CH3 , hereafter denoted mTrx-GFP (~40 kDa) and the Affibody™ ZHER2:342 ([methyl-11 C]-ZHER2:342 -ST-CH3 ) hereafter denoted ZHER2:342 (~7 kDa) proteins had been expressed with a C-terminus selenocysteine tag (ST) and site specifically labelled with a positron-emitting carbon-11 (11 C) (t 1/2 ≈ 20 min) methyl group (CH3 ). .. The widely employed 2-deoxy-2-[18 F]fluoro-D-glucose [18 F]FDG (~0.18 kDa) also used here was obtained in an aliquot from batches made daily for clinical PET at the Karolinska University Hospital.

Irradiation:

Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors
Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP. .. After 3 h, each sample was irradiated with 980 nm laser diode (OEM, CW) of 10 kW/cm2 with a spot size of 1 mm for 30 min, after which the samples were left overnight at 37 °C.

Mouse Assay:

Article Title: Fluorescent Affibody Peptide Penetration in Glioma Margin Is Superior to Full Antibody
Article Snippet: Results . shows the GFP outlined tumor, H & E staining of the same tissue section and raw fluorescence at both the Affibody and cetuximab channels. .. Control mice that received tumor implantations but no protein injections were used to determine background tissue signals.

Article Title: A method for comparing intra-tumoural radioactivity uptake heterogeneity in preclinical positron emission tomography studies
Article Snippet: In brief, the animals analysed here were severe combined immunodeficiency (SCID) mice carrying subcutaneous tumour xenografts of head and neck (FaDu) and epidermal (A431) and ovarian (SKOV-3) carcinoma cell lines. .. For radioligands, the methyl-11 C-radiolabelled Annexin A5, [methyl-11 C]-His6 -AnxA5-ST-CH3 , hereafter denoted AnxA5 (~38 kDa), mutated-thioredoxin-green fluorescence protein [methyl-11 C]-His6 -mTrx-GFP-ST-CH3 , hereafter denoted mTrx-GFP (~40 kDa) and the Affibody™ ZHER2:342 ([methyl-11 C]-ZHER2:342 -ST-CH3 ) hereafter denoted ZHER2:342 (~7 kDa) proteins had been expressed with a C-terminus selenocysteine tag (ST) and site specifically labelled with a positron-emitting carbon-11 (11 C) (t 1/2 ≈ 20 min) methyl group (CH3 ).

Concentration Assay:

Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors
Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP. .. The fluorescence in the lysis buffer was measured using the plate reader at 572 nm, and affibody concentration was determined by comparison to a calibration curve.

Generated:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: .. IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. ..

Diffusion-based Assay:

Article Title: Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding
Article Snippet: Correlation between dye properties and diffusion coefficient was assessed by measuring the closeness of fit to a linear relationship. .. These data are plotted in , which shows a strong correlation between logD and spot density, confirming the association between hydrophobicity and non-specific dye binding to the substrate. shows the distribution of D values for EGFR-GFP and affibody-dye conjugates selected for high, medium, low, and very low levels of mobility (Alexa Fluor 488, CF 633, Alexa Fluor 546, and Atto 647N, respectively).

Plasmid Preparation:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. .. Phage that bind target during panning process can then be recovered, sequenced and transferred to a periplasmic expression vector to obtain the final VHH protein from E. coli expression.

Expressing:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. .. Phage that bind target during panning process can then be recovered, sequenced and transferred to a periplasmic expression vector to obtain the final VHH protein from E. coli expression.

Modification:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: In principle, modification sites should be kept far away from the Fabs to achieved the best result. ) .. IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs.

cDNA Library Assay:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. .. For example, to obtain useful VHHs found in camelids ( B), a cDNA library can be obtained from an immunized llama or alpaca to serve as a pool of amplified DNA fragments for generation of the phage display library [ ].

Lysis:

Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors
Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP. .. At the respective time points, the spheroids were washed with PBS for 5 min and lysed in 200 μ L of lysis buffer.

Binding Assay:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: .. IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. ..

Article Title: Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding
Article Snippet: .. These data are plotted in , which shows a strong correlation between logD and spot density, confirming the association between hydrophobicity and non-specific dye binding to the substrate. shows the distribution of D values for EGFR-GFP and affibody-dye conjugates selected for high, medium, low, and very low levels of mobility (Alexa Fluor 488, CF 633, Alexa Fluor 546, and Atto 647N, respectively). ..

Recombinant:

Article Title: Current Conjugation Methods for Immunosensors
Article Snippet: .. IgG2a monoclonal antibody with two heavy chains colored in green and two light chains colored in blue (PDB:1IGT) [ ) The green fluorescent protein (GFP)-VHH (PDB: 3OGO) [ ) DARPin against tubulin beta chain (PDB: 4DUI) [ ) anti-fluorescein ScFv (PDB: 1X9Q) [ ) human epidermal growth factor receptor 2 (HER2) binding affibody (PDB: 2KZJ) [ ) 5-hydroxytryptophan aptmer (PDB: 5KPY) [ Although full-size Abs generated through immunization have been widely used for immunosensors since the beginning of biosensor development, recently developed recombinant molecules generated in vitro have many advantages over conventional Abs. ..

Positron Emission Tomography:

Article Title: A method for comparing intra-tumoural radioactivity uptake heterogeneity in preclinical positron emission tomography studies
Article Snippet: For radioligands, the methyl-11 C-radiolabelled Annexin A5, [methyl-11 C]-His6 -AnxA5-ST-CH3 , hereafter denoted AnxA5 (~38 kDa), mutated-thioredoxin-green fluorescence protein [methyl-11 C]-His6 -mTrx-GFP-ST-CH3 , hereafter denoted mTrx-GFP (~40 kDa) and the Affibody™ ZHER2:342 ([methyl-11 C]-ZHER2:342 -ST-CH3 ) hereafter denoted ZHER2:342 (~7 kDa) proteins had been expressed with a C-terminus selenocysteine tag (ST) and site specifically labelled with a positron-emitting carbon-11 (11 C) (t 1/2 ≈ 20 min) methyl group (CH3 ). .. The widely employed 2-deoxy-2-[18 F]fluoro-D-glucose [18 F]FDG (~0.18 kDa) also used here was obtained in an aliquot from batches made daily for clinical PET at the Karolinska University Hospital.

Staining:

Article Title: Fluorescent Affibody Peptide Penetration in Glioma Margin Is Superior to Full Antibody
Article Snippet: .. Results . shows the GFP outlined tumor, H & E staining of the same tissue section and raw fluorescence at both the Affibody and cetuximab channels. .. The most striking observation of the Affibody and cetuximab maps is that on average cetuximab appears to be more confined to tumor interiors , while the Affibody appears more evenly dispersed throughout the tumor ( ).

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    Affibody mtrx gfp
    PET transaxial images ( a , b , the colour scales are the same), histograms ( c , d ) of the heterogeneity contributions (the mean intensity deviation per distance calculated according to Eq. 2 ) and surface plots ( e , f ) of the uptake of AnxA5 and <t>mTrx-GFP</t> in a FaDu xenograft. The imaging was performed in the same animal > 2 h apart on the same day. In e and f , the X - and Y -axes represent spatial dimensions and the Z -axis is the mean tracer uptake (SUV mean )
    Mtrx Gfp, supplied by Affibody, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtrx gfp/product/Affibody
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mtrx gfp - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Affibody gfp egfr expressive 4t1 spheroids
    (A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected <t>4T1</t> cells grown either with (right) or without (left) 15 μ g/mL cumate to induce <t>EGFR</t> and <t>GFP</t> expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.
    Gfp Egfr Expressive 4t1 Spheroids, supplied by Affibody, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp egfr expressive 4t1 spheroids/product/Affibody
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp egfr expressive 4t1 spheroids - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    78
    Affibody mutated thioredoxin green fluorescence protein methyl 11 c his6 mtrx gfp st ch3
    (A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected <t>4T1</t> cells grown either with (right) or without (left) 15 μ g/mL cumate to induce <t>EGFR</t> and <t>GFP</t> expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.
    Mutated Thioredoxin Green Fluorescence Protein Methyl 11 C His6 Mtrx Gfp St Ch3, supplied by Affibody, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mutated thioredoxin green fluorescence protein methyl 11 c his6 mtrx gfp st ch3/product/Affibody
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mutated thioredoxin green fluorescence protein methyl 11 c his6 mtrx gfp st ch3 - by Bioz Stars, 2020-02
    78/100 stars
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    79
    Affibody egfr gfp
    (A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected <t>4T1</t> cells grown either with (right) or without (left) 15 μ g/mL cumate to induce <t>EGFR</t> and <t>GFP</t> expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.
    Egfr Gfp, supplied by Affibody, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr gfp/product/Affibody
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    egfr gfp - by Bioz Stars, 2020-02
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    Image Search Results


    PET transaxial images ( a , b , the colour scales are the same), histograms ( c , d ) of the heterogeneity contributions (the mean intensity deviation per distance calculated according to Eq. 2 ) and surface plots ( e , f ) of the uptake of AnxA5 and mTrx-GFP in a FaDu xenograft. The imaging was performed in the same animal > 2 h apart on the same day. In e and f , the X - and Y -axes represent spatial dimensions and the Z -axis is the mean tracer uptake (SUV mean )

    Journal: EJNMMI Physics

    Article Title: A method for comparing intra-tumoural radioactivity uptake heterogeneity in preclinical positron emission tomography studies

    doi: 10.1186/s40658-015-0124-1

    Figure Lengend Snippet: PET transaxial images ( a , b , the colour scales are the same), histograms ( c , d ) of the heterogeneity contributions (the mean intensity deviation per distance calculated according to Eq. 2 ) and surface plots ( e , f ) of the uptake of AnxA5 and mTrx-GFP in a FaDu xenograft. The imaging was performed in the same animal > 2 h apart on the same day. In e and f , the X - and Y -axes represent spatial dimensions and the Z -axis is the mean tracer uptake (SUV mean )

    Article Snippet: For radioligands, the methyl-11 C-radiolabelled Annexin A5, [methyl-11 C]-His6 -AnxA5-ST-CH3 , hereafter denoted AnxA5 (~38 kDa), mutated-thioredoxin-green fluorescence protein [methyl-11 C]-His6 -mTrx-GFP-ST-CH3 , hereafter denoted mTrx-GFP (~40 kDa) and the Affibody™ ZHER2:342 ([methyl-11 C]-ZHER2:342 -ST-CH3 ) hereafter denoted ZHER2:342 (~7 kDa) proteins had been expressed with a C-terminus selenocysteine tag (ST) and site specifically labelled with a positron-emitting carbon-11 (11 C) (t 1/2 ≈ 20 min) methyl group (CH3 ).

    Techniques: Positron Emission Tomography, Imaging

    (A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected 4T1 cells grown either with (right) or without (left) 15 μ g/mL cumate to induce EGFR and GFP expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.

    Journal: Journal of the American Chemical Society

    Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors

    doi: 10.1021/jacs.8b07601

    Figure Lengend Snippet: (A) Top: Composite brightfield and fluorescence microscopy images of spheroids formed from transfected 4T1 cells grown either with (right) or without (left) 15 μ g/mL cumate to induce EGFR and GFP expression. Bottom: Fluorescence images of cryotome-sectioned similarly prepared spheroids showing the distribution of fluorescence throughout the interior. All images were captured and viewed under identical settings for comparison. (B) Western blot for EGFR expression of the combined lysates of five spheroids grown either with (+) or without (−) 15 μ g/mL cumate. Image shows composite overlay of both bright field imaging for display of prestained protein ladders (L) and chemiluminescence imaging for the anti-EGFR antibody. The molecular weight of each ladder band is indicated to the right.

    Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP.

    Techniques: Fluorescence, Microscopy, Transfection, Expressing, Western Blot, Imaging, Molecular Weight

    (A) Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) showing photo-cross-linking products of either N23BP or WT to EGFR, with or without irradiation at 980 nm. Note that only 980 nm irradiation of N23BP produced a photoproduct significantly different than free EGFR (right lane). Ladder proteins are (top to bottom) 100, 75, and 50 kDa. (B) Retention of fluorescently labeled affibodies (left, N23BP; right, WT) in 3D tumor spheroids grown from transfected 4T1 cells either induced with 15 μ g/mL cumate. Concentrations of retained affibodies were calculated by comparing spheroid lysate fluorescence to standard curves prepared for each labeled affibody. “20 IR” designates that this sample was irradiated at 980 nm after 3 h incubation, then left to grow to a total of 20 h. Lanes without an IR designation were not irradiated.

    Journal: Journal of the American Chemical Society

    Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors

    doi: 10.1021/jacs.8b07601

    Figure Lengend Snippet: (A) Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) showing photo-cross-linking products of either N23BP or WT to EGFR, with or without irradiation at 980 nm. Note that only 980 nm irradiation of N23BP produced a photoproduct significantly different than free EGFR (right lane). Ladder proteins are (top to bottom) 100, 75, and 50 kDa. (B) Retention of fluorescently labeled affibodies (left, N23BP; right, WT) in 3D tumor spheroids grown from transfected 4T1 cells either induced with 15 μ g/mL cumate. Concentrations of retained affibodies were calculated by comparing spheroid lysate fluorescence to standard curves prepared for each labeled affibody. “20 IR” designates that this sample was irradiated at 980 nm after 3 h incubation, then left to grow to a total of 20 h. Lanes without an IR designation were not irradiated.

    Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP.

    Techniques: Polyacrylamide Gel Electrophoresis, SDS Page, Irradiation, Produced, Labeling, Transfection, Fluorescence, Incubation

    Left: Spheroids were treated with 1 μ M N23BP (top) or WT (bottom) affibody for a total of 4 h (left column) and 20 h (middle and right columns) and additionally irradiated with 365 nm light for 30 min after 3.5 h incubation (right column). These spheroids were sectioned at approximately the same depth and imaged for Rhodamine and GFP distribution. Micrographs show Rhodamine signal within each section normalized by the average GFP intensity. Scale bars are 200 μ m. Right: Five spheroids were grown and irradiated for 1 h with the indicated N23BP affibody concentration in growth media. Affibody containing media was removed, and spheroids were lysed and loaded onto a PAGE gel and probed for affibody conjugates using an anti-T7 antibody. High molecular weight bands around the expected molecular weight of EGFR were observed only when the spheroid-affibody mixtures were irradiated, indicating photo-cross-linking.

    Journal: Journal of the American Chemical Society

    Article Title: Anti-EGFR Affibodies with Site-Specific Photo-Cross-Linker Incorporation Show Both Directed Target-Specific Photoconjugation and Increased Retention in Tumors

    doi: 10.1021/jacs.8b07601

    Figure Lengend Snippet: Left: Spheroids were treated with 1 μ M N23BP (top) or WT (bottom) affibody for a total of 4 h (left column) and 20 h (middle and right columns) and additionally irradiated with 365 nm light for 30 min after 3.5 h incubation (right column). These spheroids were sectioned at approximately the same depth and imaged for Rhodamine and GFP distribution. Micrographs show Rhodamine signal within each section normalized by the average GFP intensity. Scale bars are 200 μ m. Right: Five spheroids were grown and irradiated for 1 h with the indicated N23BP affibody concentration in growth media. Affibody containing media was removed, and spheroids were lysed and loaded onto a PAGE gel and probed for affibody conjugates using an anti-T7 antibody. High molecular weight bands around the expected molecular weight of EGFR were observed only when the spheroid-affibody mixtures were irradiated, indicating photo-cross-linking.

    Article Snippet: GFP-EGFR expressive 4T1 spheroids in a 96-well plate were mixed with either 1 μ M Rh-conjugated N23C-BP or Rh-WT affibody and 100 μ g/mL of UCNP.

    Techniques: Irradiation, Incubation, Concentration Assay, Polyacrylamide Gel Electrophoresis, Molecular Weight