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OriGene gfp expressing plasmid
Gfp Expressing Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp expressing plasmid/product/OriGene
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gfp expressing plasmid - by Bioz Stars, 2020-08
89/100 stars

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Article Title: Loss of Expression and Function of SOCS3 Is an Early Event in HNSCC: Altered Subcellular Localization as a Possible Mechanism Involved in Proliferation, Migration and Invasion
Article Snippet: .. SOCS3 expression plasmids and the empty control vector and GFP-expressing plasmid were obtained from Origene (Origene Technologies Inc., Rockville, MD). .. SOCS3 pool siRNA was from Dharmacon (Thermo Fisher Scientific Inc., Lafayette, CO).

Plasmid Preparation:

Article Title: Loss of Expression and Function of SOCS3 Is an Early Event in HNSCC: Altered Subcellular Localization as a Possible Mechanism Involved in Proliferation, Migration and Invasion
Article Snippet: .. SOCS3 expression plasmids and the empty control vector and GFP-expressing plasmid were obtained from Origene (Origene Technologies Inc., Rockville, MD). .. SOCS3 pool siRNA was from Dharmacon (Thermo Fisher Scientific Inc., Lafayette, CO).

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  • 90
    OriGene pp5 expression construct a green fluorescent protein gfp pp5 expression construct
    <t>PP5</t> increases CD70 and KIR expression in CD4 + T cells. CD4 + T cells were stimulated with PHA, transfected with the <t>PP5-GFP</t> expression construct then CD70 and KIR mRNA and protein levels were measured 3 days later. A - CD70 mRNA levels. N=7, p=0.03. B - CD70 protein levels. N=3, p
    Pp5 Expression Construct A Green Fluorescent Protein Gfp Pp5 Expression Construct, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp5 expression construct a green fluorescent protein gfp pp5 expression construct/product/OriGene
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pp5 expression construct a green fluorescent protein gfp pp5 expression construct - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    91
    OriGene glut4 gfp expression vector
    (A) Insulin mobilization of <t>GLUT4</t> in mPMVEC is decreased in BMPR2 R899X mice, and decreased further by pretreatment with 16αOHE1. Insulin mobilization was determined by counting perinuclear vs membrane associated <t>GFP-tagged</t> GLUT4 in each of 100 cells in each of three technical replicates (% in each replicate indicated by circles). Every group has three technical replicates: some are overlapping. Error bars are standard deviation; differences are significant at p
    Glut4 Gfp Expression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glut4 gfp expression vector/product/OriGene
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glut4 gfp expression vector - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    90
    OriGene gfp expressing cell lines human aqp1
    <t>AQP1</t> reports gene expression over a large dynamic range. ( a ) Diffusion-weighted images (acquired at Δ eff =398 ms, b =2,089 s mm −2 ) of CHO cells expressing AQP1 or <t>GFP</t> (control) and treated with varying doses of doxycycline to induce transgene expression. Scale bar, 3 mm. ( b ) Percent change in ADC of water in AQP1-expressing CHO cells (relative to control cells expressing GFP) as a function of doxycycline concentration, measured at different diffusion times. n =4 biological replicates. Error bars±s.e.m. ( c ) Levels of AQP1 expression on the membrane of CHO cells at different levels of doxycycline induction, estimated based on quantitative western blotting and relative expression of a co-transcribed GFP reporter, compared with values calculated from Monte Carlo simulations based on the ADC results in b . n ≥3 biological replicates. Error bars±s.e.m.
    Gfp Expressing Cell Lines Human Aqp1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp expressing cell lines human aqp1/product/OriGene
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp expressing cell lines human aqp1 - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    OriGene gfp expression vector
    Targeting of angiogenic ECs with <t>CD44</t> or EphA2 siRNA blocks in vivo LMW-HA-mediated angiogenesis. Panel A , Matrigel plugs from mice with intravenous administration of anginex-conjugated liposomes containing either pCMV6 empty vector or <t>pCMV6-A-GFP</t> (Origene)
    Gfp Expression Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp expression vector/product/OriGene
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    gfp expression vector - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    PP5 increases CD70 and KIR expression in CD4 + T cells. CD4 + T cells were stimulated with PHA, transfected with the PP5-GFP expression construct then CD70 and KIR mRNA and protein levels were measured 3 days later. A - CD70 mRNA levels. N=7, p=0.03. B - CD70 protein levels. N=3, p

    Journal: Lupus (Los Angeles)

    Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus

    doi:

    Figure Lengend Snippet: PP5 increases CD70 and KIR expression in CD4 + T cells. CD4 + T cells were stimulated with PHA, transfected with the PP5-GFP expression construct then CD70 and KIR mRNA and protein levels were measured 3 days later. A - CD70 mRNA levels. N=7, p=0.03. B - CD70 protein levels. N=3, p

    Article Snippet: PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF.

    Techniques: Expressing, Transfection, Construct

    PP5 decreases Dnmt1 levels in CD4 + T cells. CD4 + T cells from healthy subjects were stimulated with PHA, transfected with a PP5-GFP expression construct and Dnmt1 mRNA levels measured 24–36 h later. N=3, *p=0.02.

    Journal: Lupus (Los Angeles)

    Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus

    doi:

    Figure Lengend Snippet: PP5 decreases Dnmt1 levels in CD4 + T cells. CD4 + T cells from healthy subjects were stimulated with PHA, transfected with a PP5-GFP expression construct and Dnmt1 mRNA levels measured 24–36 h later. N=3, *p=0.02.

    Article Snippet: PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF.

    Techniques: Transfection, Expressing, Construct

    PP5 increases perforin and CD11a expression in CD4 + T cells. CD4 + T cells were stimulated with PHA, transfected with the PP5-GFP expression construct then A - perforin (N=4, p=0.03) and B - CD11a (N=5, *P=0.047) mRNA levels were measured 3 days later.

    Journal: Lupus (Los Angeles)

    Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus

    doi:

    Figure Lengend Snippet: PP5 increases perforin and CD11a expression in CD4 + T cells. CD4 + T cells were stimulated with PHA, transfected with the PP5-GFP expression construct then A - perforin (N=4, p=0.03) and B - CD11a (N=5, *P=0.047) mRNA levels were measured 3 days later.

    Article Snippet: PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF.

    Techniques: Expressing, Transfection, Construct

    PP5 increases CD40L expression in CD4 + T cells. Female CD4 + T cells were stimulated with PHA, transfected with the PP5-GFP expression construct then CD40L surface protein was measured 3 days later. N=3, *P

    Journal: Lupus (Los Angeles)

    Article Title: Protein Phosphatase 5 Contributes to the Overexpression of Epigenetically Regulated T-Lymphocyte Genes in Patients with Lupus

    doi:

    Figure Lengend Snippet: PP5 increases CD40L expression in CD4 + T cells. Female CD4 + T cells were stimulated with PHA, transfected with the PP5-GFP expression construct then CD40L surface protein was measured 3 days later. N=3, *P

    Article Snippet: PP5 expression construct A green fluorescent protein (GFP) PP5 expression construct was generated by cloning the PP5c open reading frame (ORF) construct (Origene) into the pCMV6-GFP vector (Origene) at the EcoRI and BamHI sites, immediately upstream of the GFP ORF.

    Techniques: Expressing, Transfection, Construct

    (A) Insulin mobilization of GLUT4 in mPMVEC is decreased in BMPR2 R899X mice, and decreased further by pretreatment with 16αOHE1. Insulin mobilization was determined by counting perinuclear vs membrane associated GFP-tagged GLUT4 in each of 100 cells in each of three technical replicates (% in each replicate indicated by circles). Every group has three technical replicates: some are overlapping. Error bars are standard deviation; differences are significant at p

    Journal: The European respiratory journal

    Article Title: Estrogen Inhibition Reverses Pulmonary Arterial Hypertension and Associated Metabolic Defects

    doi: 10.1183/13993003.02337-2016

    Figure Lengend Snippet: (A) Insulin mobilization of GLUT4 in mPMVEC is decreased in BMPR2 R899X mice, and decreased further by pretreatment with 16αOHE1. Insulin mobilization was determined by counting perinuclear vs membrane associated GFP-tagged GLUT4 in each of 100 cells in each of three technical replicates (% in each replicate indicated by circles). Every group has three technical replicates: some are overlapping. Error bars are standard deviation; differences are significant at p

    Article Snippet: Glut4-GFP expression vector was purchased from OriGene (Rockville, MD).

    Techniques: Mouse Assay, Standard Deviation

    AQP1 reports gene expression over a large dynamic range. ( a ) Diffusion-weighted images (acquired at Δ eff =398 ms, b =2,089 s mm −2 ) of CHO cells expressing AQP1 or GFP (control) and treated with varying doses of doxycycline to induce transgene expression. Scale bar, 3 mm. ( b ) Percent change in ADC of water in AQP1-expressing CHO cells (relative to control cells expressing GFP) as a function of doxycycline concentration, measured at different diffusion times. n =4 biological replicates. Error bars±s.e.m. ( c ) Levels of AQP1 expression on the membrane of CHO cells at different levels of doxycycline induction, estimated based on quantitative western blotting and relative expression of a co-transcribed GFP reporter, compared with values calculated from Monte Carlo simulations based on the ADC results in b . n ≥3 biological replicates. Error bars±s.e.m.

    Journal: Nature Communications

    Article Title: Non-invasive imaging using reporter genes altering cellular water permeability

    doi: 10.1038/ncomms13891

    Figure Lengend Snippet: AQP1 reports gene expression over a large dynamic range. ( a ) Diffusion-weighted images (acquired at Δ eff =398 ms, b =2,089 s mm −2 ) of CHO cells expressing AQP1 or GFP (control) and treated with varying doses of doxycycline to induce transgene expression. Scale bar, 3 mm. ( b ) Percent change in ADC of water in AQP1-expressing CHO cells (relative to control cells expressing GFP) as a function of doxycycline concentration, measured at different diffusion times. n =4 biological replicates. Error bars±s.e.m. ( c ) Levels of AQP1 expression on the membrane of CHO cells at different levels of doxycycline induction, estimated based on quantitative western blotting and relative expression of a co-transcribed GFP reporter, compared with values calculated from Monte Carlo simulations based on the ADC results in b . n ≥3 biological replicates. Error bars±s.e.m.

    Article Snippet: Construction of aquaporin and GFP-expressing cell lines Human AQP1 (NM_198098.1) and AQP4 (NM_001650.4) complementary DNAs were ordered from OriGene (Rockville, MD) and subcloned into a lentiviral vector downstream of a constitutive CMV or doxycycline-regulated CMV promoter (Clontech, Mountain View, CA) and an N-terminal FLAG tag.

    Techniques: Expressing, Diffusion-based Assay, Mass Spectrometry, Concentration Assay, Western Blot

    AQP1 functions as a genetically encoded reporter for diffusion-weighted MRI. ( a ) Illustration of the impact of aquaporin expression on water diffusion across the cell membrane and the resulting decrease in diffusion-weighted signal intensity. ( b ) Diffusion-weighted images of CHO, U87 and Neuro 2a cell pellets expressing AQP1 or GFP, acquired using a b -value of ∼1,000 s mm −2 . Scale bars, 3 mm. ( c ) ADC of water in CHO, U87 and Neuro 2a cells expressing AQP1 relative to GFP controls, measured at Δ eff =398 ms. Transgene expression in CHO cells was induced with 1 μg ml −1 doxycycline, whereas U87 and Neuro 2a cells express AQP1 from a constitutive promoter. n =4 (U87, Neuro 2a) and 5 (CHO) biological replicates. ( d ) Longitudinal ( T 1 ) and ( e ) transverse ( T 2 ) relaxation rates in cells expressing AQP1 or GFP. n =3 (Neuro 2a, CHO) or 4 (U87) biological replicates. ( f ) Cell viability on AQP1 or GFP expression. n =12 (resazurin assay), 6 (ATP content), 4 (LDH release) and 3 (ethidium staining) biological replicates. Error bars±s.e.m. ( g ) Phase-contrast images of CHO, U87 and Neuro 2a cells expressing AQP1 or GFP. Scale bars, 10 μm.

    Journal: Nature Communications

    Article Title: Non-invasive imaging using reporter genes altering cellular water permeability

    doi: 10.1038/ncomms13891

    Figure Lengend Snippet: AQP1 functions as a genetically encoded reporter for diffusion-weighted MRI. ( a ) Illustration of the impact of aquaporin expression on water diffusion across the cell membrane and the resulting decrease in diffusion-weighted signal intensity. ( b ) Diffusion-weighted images of CHO, U87 and Neuro 2a cell pellets expressing AQP1 or GFP, acquired using a b -value of ∼1,000 s mm −2 . Scale bars, 3 mm. ( c ) ADC of water in CHO, U87 and Neuro 2a cells expressing AQP1 relative to GFP controls, measured at Δ eff =398 ms. Transgene expression in CHO cells was induced with 1 μg ml −1 doxycycline, whereas U87 and Neuro 2a cells express AQP1 from a constitutive promoter. n =4 (U87, Neuro 2a) and 5 (CHO) biological replicates. ( d ) Longitudinal ( T 1 ) and ( e ) transverse ( T 2 ) relaxation rates in cells expressing AQP1 or GFP. n =3 (Neuro 2a, CHO) or 4 (U87) biological replicates. ( f ) Cell viability on AQP1 or GFP expression. n =12 (resazurin assay), 6 (ATP content), 4 (LDH release) and 3 (ethidium staining) biological replicates. Error bars±s.e.m. ( g ) Phase-contrast images of CHO, U87 and Neuro 2a cells expressing AQP1 or GFP. Scale bars, 10 μm.

    Article Snippet: Construction of aquaporin and GFP-expressing cell lines Human AQP1 (NM_198098.1) and AQP4 (NM_001650.4) complementary DNAs were ordered from OriGene (Rockville, MD) and subcloned into a lentiviral vector downstream of a constitutive CMV or doxycycline-regulated CMV promoter (Clontech, Mountain View, CA) and an N-terminal FLAG tag.

    Techniques: Diffusion-based Assay, Magnetic Resonance Imaging, Expressing, Mass Spectrometry, Resazurin Assay, Staining

    AQP1 expression is observable in mixed cell populations. ( a ) Illustration of the effect of an increasing fraction of AQP1-labelled cells in a tissue on the overall diffusivity of water. ( b ) Monte Carlo simulation predictions of change in ADC as a function of the fraction of cells expressing AQP1 in a mixed cellular lattice. ( c ) Top: diffusion-weighted MRI (acquired at Δ eff =198 ms, b =2,334 s mm −2 ) of cells comprising AQP1-labelled cells mixed with GFP-labelled control cells in varying proportions. Bottom: image of mixed populations containing 0, 5 and 10% AQP1-expressing cells acquired using Δ eff =398 ms, b =8,000 s mm −2 , to maximize contrast for the low AQP1 fraction scenario, smoothed with a Gaussian filter (radius 1.5 pixels). Scale bars, 3 mm. ( d ) Percent change in ADC in mixed AQP1/GFP cell pellets as a function of the fraction of AQP1-expressing cells. N =4 biological replicates. Error bars±s.e.m.

    Journal: Nature Communications

    Article Title: Non-invasive imaging using reporter genes altering cellular water permeability

    doi: 10.1038/ncomms13891

    Figure Lengend Snippet: AQP1 expression is observable in mixed cell populations. ( a ) Illustration of the effect of an increasing fraction of AQP1-labelled cells in a tissue on the overall diffusivity of water. ( b ) Monte Carlo simulation predictions of change in ADC as a function of the fraction of cells expressing AQP1 in a mixed cellular lattice. ( c ) Top: diffusion-weighted MRI (acquired at Δ eff =198 ms, b =2,334 s mm −2 ) of cells comprising AQP1-labelled cells mixed with GFP-labelled control cells in varying proportions. Bottom: image of mixed populations containing 0, 5 and 10% AQP1-expressing cells acquired using Δ eff =398 ms, b =8,000 s mm −2 , to maximize contrast for the low AQP1 fraction scenario, smoothed with a Gaussian filter (radius 1.5 pixels). Scale bars, 3 mm. ( d ) Percent change in ADC in mixed AQP1/GFP cell pellets as a function of the fraction of AQP1-expressing cells. N =4 biological replicates. Error bars±s.e.m.

    Article Snippet: Construction of aquaporin and GFP-expressing cell lines Human AQP1 (NM_198098.1) and AQP4 (NM_001650.4) complementary DNAs were ordered from OriGene (Rockville, MD) and subcloned into a lentiviral vector downstream of a constitutive CMV or doxycycline-regulated CMV promoter (Clontech, Mountain View, CA) and an N-terminal FLAG tag.

    Techniques: Expressing, Diffusion-based Assay, Magnetic Resonance Imaging, Mass Spectrometry

    AQP1 enables the imaging of gene expression in intracranial tumour xenografts. ( a ) Experimental approach to establishing bilateral tumours in the striatum, inducing transgene expression, and performing diffusion-weighted MRI. ( b ) Representative diffusion-weighted image of a horizontal brain slice with bilateral tumour xenografts, 48 h after doxycycline injection. Inset shows a diffusion-weighted image of the same mouse acquired before doxycycline injection. Images were acquired at Δ eff =98 ms and b -value=1,000 s mm −2 . Dashed lines indicate the tumour ROI(s). Scale bar, 2 mm. ( c ) Average diffusion-weighted image intensity of AQP1-expressing tumours relative to contralateral GFP-expressing tumours before and after doxycycline induction. n =5 biological replicates. Error bars±s.e.m. ( d ) Confocal fluorescence image of a representative 100 μm section of a mouse brain implanted with GFP and AQP1 tumours. The AQP1 tumour appears dimmer due to diminished GFP translation from the IRES sequence. Cell nuclei are counterstained using TO-PRO iodide (red). Scale bar, 2 mm. ( e ) Low ( × 10) and ( f ) high ( × 30) magnification images of 5 μm haematoxylin–eosin-stained sections of intracranial tumour xenografts expressing AQP1 and GFP. Scale bars, 30 and 10 μm, respectively. ( g ) Longitudinal measurements of tumour growth in bilateral subcutaneous xenografts induced using doxycycline to express AQP1 or GFP 11 days following tumour inoculation. ( h ) Mean end-point tumour mass and ( i ) images of AQP1- and GFP-expressing subcutaneous tumours harvested 9 days after doxycycline induction of gene expression. n =4 biological replicates. Scale bar, 1 cm. Error bars±s.e.m.

    Journal: Nature Communications

    Article Title: Non-invasive imaging using reporter genes altering cellular water permeability

    doi: 10.1038/ncomms13891

    Figure Lengend Snippet: AQP1 enables the imaging of gene expression in intracranial tumour xenografts. ( a ) Experimental approach to establishing bilateral tumours in the striatum, inducing transgene expression, and performing diffusion-weighted MRI. ( b ) Representative diffusion-weighted image of a horizontal brain slice with bilateral tumour xenografts, 48 h after doxycycline injection. Inset shows a diffusion-weighted image of the same mouse acquired before doxycycline injection. Images were acquired at Δ eff =98 ms and b -value=1,000 s mm −2 . Dashed lines indicate the tumour ROI(s). Scale bar, 2 mm. ( c ) Average diffusion-weighted image intensity of AQP1-expressing tumours relative to contralateral GFP-expressing tumours before and after doxycycline induction. n =5 biological replicates. Error bars±s.e.m. ( d ) Confocal fluorescence image of a representative 100 μm section of a mouse brain implanted with GFP and AQP1 tumours. The AQP1 tumour appears dimmer due to diminished GFP translation from the IRES sequence. Cell nuclei are counterstained using TO-PRO iodide (red). Scale bar, 2 mm. ( e ) Low ( × 10) and ( f ) high ( × 30) magnification images of 5 μm haematoxylin–eosin-stained sections of intracranial tumour xenografts expressing AQP1 and GFP. Scale bars, 30 and 10 μm, respectively. ( g ) Longitudinal measurements of tumour growth in bilateral subcutaneous xenografts induced using doxycycline to express AQP1 or GFP 11 days following tumour inoculation. ( h ) Mean end-point tumour mass and ( i ) images of AQP1- and GFP-expressing subcutaneous tumours harvested 9 days after doxycycline induction of gene expression. n =4 biological replicates. Scale bar, 1 cm. Error bars±s.e.m.

    Article Snippet: Construction of aquaporin and GFP-expressing cell lines Human AQP1 (NM_198098.1) and AQP4 (NM_001650.4) complementary DNAs were ordered from OriGene (Rockville, MD) and subcloned into a lentiviral vector downstream of a constitutive CMV or doxycycline-regulated CMV promoter (Clontech, Mountain View, CA) and an N-terminal FLAG tag.

    Techniques: Imaging, Expressing, Diffusion-based Assay, Magnetic Resonance Imaging, Slice Preparation, Injection, Mass Spectrometry, Fluorescence, Sequencing, Staining

    Targeting of angiogenic ECs with CD44 or EphA2 siRNA blocks in vivo LMW-HA-mediated angiogenesis. Panel A , Matrigel plugs from mice with intravenous administration of anginex-conjugated liposomes containing either pCMV6 empty vector or pCMV6-A-GFP (Origene)

    Journal: The Journal of Biological Chemistry

    Article Title: Transactivation of the Receptor-tyrosine Kinase Ephrin Receptor A2 Is Required for the Low Molecular Weight Hyaluronan-mediated Angiogenesis That Is implicated in Tumor Progression *

    doi: 10.1074/jbc.M114.554766

    Figure Lengend Snippet: Targeting of angiogenic ECs with CD44 or EphA2 siRNA blocks in vivo LMW-HA-mediated angiogenesis. Panel A , Matrigel plugs from mice with intravenous administration of anginex-conjugated liposomes containing either pCMV6 empty vector or pCMV6-A-GFP (Origene)

    Article Snippet: Sterile anginex-conjugated liposomes containing no siRNA (control), CD44, EphA2, or control siRNA (100 μl) or pCMV6 empty vector or GFP expression vector (pCMV6-A-GFP Vector, Origene) were injected into the internal jugular vein of C57B6/6J mice.

    Techniques: In Vivo, Mouse Assay, Plasmid Preparation