Structured Review

InvivoGen gfp control vector
Gfp Control Vector, supplied by InvivoGen, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp control vector/product/InvivoGen
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
gfp control vector - by Bioz Stars, 2020-08
88/100 stars

Images

Related Articles

Plasmid Preparation:

Article Title: DNMT1-dependent suppression of microRNA424 regulates tumor progression in human bladder cancer
Article Snippet: .. The DNMT1-GFP silencing vector and GFP-control vector were purchased from InvivoGen, as previously described [ ]. .. The miR-424 expression vector (human miR-424 constructs in LentiIII vector) and control vector were obtained from Applied Biological Materials (ABM) Inc. (Richmond, Canada).

Article Title: Significance of DNMT3b in Oral Cancer
Article Snippet: .. The DNMT3b-GFP silencing vector (psiRNA-h7SK vector expressing siRNA targeting human DNMT3B from the human 7SK RNA polymerase III promoter, and the GFP∶Zeo fusion gene which confers both reporter and antibiotic resistance activities) and GFP-control vector (Non-effective scrambled siRNA in GFP vector) were purchased from InvivoGen. .. Stable DNMT3b-silenced cancer cells were generated by transfecting SCC4 and SCC25 cells with the DNMT3b silencing vector and selected by culturing in medium containing Zeomycin for 4 weeks.

Expressing:

Article Title: Significance of DNMT3b in Oral Cancer
Article Snippet: .. The DNMT3b-GFP silencing vector (psiRNA-h7SK vector expressing siRNA targeting human DNMT3B from the human 7SK RNA polymerase III promoter, and the GFP∶Zeo fusion gene which confers both reporter and antibiotic resistance activities) and GFP-control vector (Non-effective scrambled siRNA in GFP vector) were purchased from InvivoGen. .. Stable DNMT3b-silenced cancer cells were generated by transfecting SCC4 and SCC25 cells with the DNMT3b silencing vector and selected by culturing in medium containing Zeomycin for 4 weeks.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    InvivoGen ubilt3 gfp control cell line
    DRibbles were efficient antigen carriers for the activation of human CD8 + T cells and CD4 + T cells. PBMCs were separated according to their density into monocytes and lymphocytes by Elutra apheresis. DRibbles were collected from HEK 293 T cells that expressed E6E7 protein or CEF protein. Monocytes were loaded with 25ug/ml CEF DRibbles or 25ug/ml control E6E7 DRibbles. After 6 hours, lymphocytes were added. Activation of T cells was assessed by determining the percentage of IFN-γ + CD8 + cells (A) and IFN-γ + CD4 + cells (B) detected by ICS. The mean ± SEM of 3 separate experiments from the same donor are shown. CD8 + (C) and CD4 + T cell responses (D) against <t>UbiLT3</t> pp65 DRibbles or control UbiLT3 <t>GFP</t> DRibbles and CMV pp65 protein were analyzed in frozen-thawed PBMCs from 24 subjects. (E) shows the representative dot plot from one of the donors.
    Ubilt3 Gfp Control Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubilt3 gfp control cell line/product/InvivoGen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ubilt3 gfp control cell line - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    85
    InvivoGen control plasmid expressing gfp
    Autophagosome and lysosome levels in control and Hsp90 inhibitor-treated cells. a. Stable clones of RKO expressing <t>GFP</t> (negative control) or <t>GFP-LC3</t> (NEG-siRNA or HSF1siRNA transfected) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Fluorescent
    Control Plasmid Expressing Gfp, supplied by InvivoGen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control plasmid expressing gfp/product/InvivoGen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    control plasmid expressing gfp - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    92
    InvivoGen reporter direct repeat gfp
    UCHL3 facilitates classical NHEJ. (A) Schematic representation of a fluorescent reporter assay measuring c-NHEJ efficiency. I-SceI digestion sites and inserted stop codon are indicated by arrow heads and red characters, respectively. (B) <t>U2OS</t> cells transfected with the indicated siRNAs were subjected to c-NHEJ assay. The efficiency of c-NHEJ was normalized to control siRNA-transfected cells and set to 100% (Mean ± SEM, n = 3). (C) U2OS or UCHL3 KO#2 cells transfected with the indicated plasmid coding FLAG (empty vector: EV) or FLAG-UCHL3 were subjected to c-NHEJ assay. The efficiency of c-NHEJ was normalized to EV transfected U2OS cells and set to 100% (Mean ± SEM, n = 3). (D) U2OS cells transfected with the indicated siRNAs were subjected to direct-repeat <t>GFP</t> assay. The efficiency of homology mediated repair was normalized to control siRNA-transfected cells and set to 100% (Mean ± SEM, n = 3). (E , F) U2OS (WT) or UCHL3 KO#1 cells transfected with the indicated siRNAs were processed for immunoblotting analysis (E) or subjected to clonogenic survival assay after IR (F) (Mean ± SEM, n = 3). (G , H) U2OS cells stably expressing FLAG-UCHL3 (wild-type: WT) (G) or catalytically inactive mutant (C95A) (H) .
    Reporter Direct Repeat Gfp, supplied by InvivoGen, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reporter direct repeat gfp/product/InvivoGen
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    reporter direct repeat gfp - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    InvivoGen gfp gene
    Autophagosome and lysosome levels in control and Hsp90 inhibitor-treated cells. a. Stable clones of RKO expressing <t>GFP</t> (negative control) or <t>GFP-LC3</t> (NEG-siRNA or HSF1siRNA transfected) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Fluorescent
    Gfp Gene, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp gene/product/InvivoGen
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp gene - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    DRibbles were efficient antigen carriers for the activation of human CD8 + T cells and CD4 + T cells. PBMCs were separated according to their density into monocytes and lymphocytes by Elutra apheresis. DRibbles were collected from HEK 293 T cells that expressed E6E7 protein or CEF protein. Monocytes were loaded with 25ug/ml CEF DRibbles or 25ug/ml control E6E7 DRibbles. After 6 hours, lymphocytes were added. Activation of T cells was assessed by determining the percentage of IFN-γ + CD8 + cells (A) and IFN-γ + CD4 + cells (B) detected by ICS. The mean ± SEM of 3 separate experiments from the same donor are shown. CD8 + (C) and CD4 + T cell responses (D) against UbiLT3 pp65 DRibbles or control UbiLT3 GFP DRibbles and CMV pp65 protein were analyzed in frozen-thawed PBMCs from 24 subjects. (E) shows the representative dot plot from one of the donors.

    Journal: Journal of Translational Medicine

    Article Title: Cross-presentation of viral antigens in dribbles leads to efficient activation of virus-specific human memory t cells

    doi: 10.1186/1479-5876-12-100

    Figure Lengend Snippet: DRibbles were efficient antigen carriers for the activation of human CD8 + T cells and CD4 + T cells. PBMCs were separated according to their density into monocytes and lymphocytes by Elutra apheresis. DRibbles were collected from HEK 293 T cells that expressed E6E7 protein or CEF protein. Monocytes were loaded with 25ug/ml CEF DRibbles or 25ug/ml control E6E7 DRibbles. After 6 hours, lymphocytes were added. Activation of T cells was assessed by determining the percentage of IFN-γ + CD8 + cells (A) and IFN-γ + CD4 + cells (B) detected by ICS. The mean ± SEM of 3 separate experiments from the same donor are shown. CD8 + (C) and CD4 + T cell responses (D) against UbiLT3 pp65 DRibbles or control UbiLT3 GFP DRibbles and CMV pp65 protein were analyzed in frozen-thawed PBMCs from 24 subjects. (E) shows the representative dot plot from one of the donors.

    Article Snippet: The UbiLT3 pp65 and UbiLT3 GFP control cell line were generated by transfection of UbiLT3 with pp65 plasmid vector or control vector encoding GFP protein and two weeks selection with 2 μg/ml puromycin (Invivogen, ant-pr-1).

    Techniques: Activation Assay

    Autophagosome and lysosome levels in control and Hsp90 inhibitor-treated cells. a. Stable clones of RKO expressing GFP (negative control) or GFP-LC3 (NEG-siRNA or HSF1siRNA transfected) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Fluorescent

    Journal: Biochemical pharmacology

    Article Title: Heat Shock Factor 1 Confers Resistance to Hsp90 Inhibitors through p62/SQSTM1 Expression and Promotion of Autophagic Flux

    doi: 10.1016/j.bcp.2013.11.014

    Figure Lengend Snippet: Autophagosome and lysosome levels in control and Hsp90 inhibitor-treated cells. a. Stable clones of RKO expressing GFP (negative control) or GFP-LC3 (NEG-siRNA or HSF1siRNA transfected) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Fluorescent

    Article Snippet: For visualizing autophagosomes, an expression construct containing the human LC3B gene fused at 5’ end to the GFP gene (pSELECT-GFP-LC3) and control plasmid expressing GFP alone (pSELECT-NGFP-zeo) were both obtained from InvivoGen.

    Techniques: Clone Assay, Expressing, Negative Control, Transfection

    UCHL3 facilitates classical NHEJ. (A) Schematic representation of a fluorescent reporter assay measuring c-NHEJ efficiency. I-SceI digestion sites and inserted stop codon are indicated by arrow heads and red characters, respectively. (B) U2OS cells transfected with the indicated siRNAs were subjected to c-NHEJ assay. The efficiency of c-NHEJ was normalized to control siRNA-transfected cells and set to 100% (Mean ± SEM, n = 3). (C) U2OS or UCHL3 KO#2 cells transfected with the indicated plasmid coding FLAG (empty vector: EV) or FLAG-UCHL3 were subjected to c-NHEJ assay. The efficiency of c-NHEJ was normalized to EV transfected U2OS cells and set to 100% (Mean ± SEM, n = 3). (D) U2OS cells transfected with the indicated siRNAs were subjected to direct-repeat GFP assay. The efficiency of homology mediated repair was normalized to control siRNA-transfected cells and set to 100% (Mean ± SEM, n = 3). (E , F) U2OS (WT) or UCHL3 KO#1 cells transfected with the indicated siRNAs were processed for immunoblotting analysis (E) or subjected to clonogenic survival assay after IR (F) (Mean ± SEM, n = 3). (G , H) U2OS cells stably expressing FLAG-UCHL3 (wild-type: WT) (G) or catalytically inactive mutant (C95A) (H) .

    Journal: Scientific Reports

    Article Title: The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage

    doi: 10.1038/s41598-018-36235-0

    Figure Lengend Snippet: UCHL3 facilitates classical NHEJ. (A) Schematic representation of a fluorescent reporter assay measuring c-NHEJ efficiency. I-SceI digestion sites and inserted stop codon are indicated by arrow heads and red characters, respectively. (B) U2OS cells transfected with the indicated siRNAs were subjected to c-NHEJ assay. The efficiency of c-NHEJ was normalized to control siRNA-transfected cells and set to 100% (Mean ± SEM, n = 3). (C) U2OS or UCHL3 KO#2 cells transfected with the indicated plasmid coding FLAG (empty vector: EV) or FLAG-UCHL3 were subjected to c-NHEJ assay. The efficiency of c-NHEJ was normalized to EV transfected U2OS cells and set to 100% (Mean ± SEM, n = 3). (D) U2OS cells transfected with the indicated siRNAs were subjected to direct-repeat GFP assay. The efficiency of homology mediated repair was normalized to control siRNA-transfected cells and set to 100% (Mean ± SEM, n = 3). (E , F) U2OS (WT) or UCHL3 KO#1 cells transfected with the indicated siRNAs were processed for immunoblotting analysis (E) or subjected to clonogenic survival assay after IR (F) (Mean ± SEM, n = 3). (G , H) U2OS cells stably expressing FLAG-UCHL3 (wild-type: WT) (G) or catalytically inactive mutant (C95A) (H) .

    Article Snippet: U2OS stably expressing the HR reporter direct repeat-GFP, EJ2-GFP reporter and U2OS UCHL3 knock out cell lines were cultured in identical media with 1 μg/ml puromycin (InvivoGen) instead of Geneticin.

    Techniques: Non-Homologous End Joining, Reporter Assay, Transfection, Plasmid Preparation, Clonogenic Cell Survival Assay, Stable Transfection, Expressing, Mutagenesis

    UCHL3 directly deubiquitylates Ku80 ubiquitylation that is dependent on DSB and downstream NHEJ factors. (A) Purified GST or GST-UCHL3 was subjected to in vitro deubiquitylating enzyme activity assay, verifying purified UCHL3 is enzymatically active. An asterisk indicates HA-Ub-Vs bound UCHL3. (B) Silver staining of purified Ku used for electrophoretic mobility shift assay. (C) Electrophoretic mobility shift assay was carried out with purified Ku and UCHL3 proteins. The fluorescein labelled double-strand DNA (dsDNA, 50 nM) was incubated with the purified Ku (100 nM) in combination either with GST (600 nM) or GST-UCHL3 (300 or 600 nM). Free dsDNA and dsDNA bound with Ku are indicated. (D) GFP-Ku80 was immune-purified from GFP-Ku80 expressing-U2OS cells treated with phleomycin. Purified GFP-Ku80 was subjected to in vitro deubiquitylation assay. GFP-Ku80 bound beads were incubated either with GST or GST-UCHL3 and analysed by immunoblotting with the indicated antibodies. The bracket indicates ubiquitylated Ku80. (E) U2OS cells stably expressing GFP or GFP-Ku80 were subjected to in vivo .

    Journal: Scientific Reports

    Article Title: The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage

    doi: 10.1038/s41598-018-36235-0

    Figure Lengend Snippet: UCHL3 directly deubiquitylates Ku80 ubiquitylation that is dependent on DSB and downstream NHEJ factors. (A) Purified GST or GST-UCHL3 was subjected to in vitro deubiquitylating enzyme activity assay, verifying purified UCHL3 is enzymatically active. An asterisk indicates HA-Ub-Vs bound UCHL3. (B) Silver staining of purified Ku used for electrophoretic mobility shift assay. (C) Electrophoretic mobility shift assay was carried out with purified Ku and UCHL3 proteins. The fluorescein labelled double-strand DNA (dsDNA, 50 nM) was incubated with the purified Ku (100 nM) in combination either with GST (600 nM) or GST-UCHL3 (300 or 600 nM). Free dsDNA and dsDNA bound with Ku are indicated. (D) GFP-Ku80 was immune-purified from GFP-Ku80 expressing-U2OS cells treated with phleomycin. Purified GFP-Ku80 was subjected to in vitro deubiquitylation assay. GFP-Ku80 bound beads were incubated either with GST or GST-UCHL3 and analysed by immunoblotting with the indicated antibodies. The bracket indicates ubiquitylated Ku80. (E) U2OS cells stably expressing GFP or GFP-Ku80 were subjected to in vivo .

    Article Snippet: U2OS stably expressing the HR reporter direct repeat-GFP, EJ2-GFP reporter and U2OS UCHL3 knock out cell lines were cultured in identical media with 1 μg/ml puromycin (InvivoGen) instead of Geneticin.

    Techniques: Non-Homologous End Joining, Purification, In Vitro, Enzyme Activity Assay, Silver Staining, Electrophoretic Mobility Shift Assay, Incubation, Expressing, Stable Transfection, In Vivo

    UCHL3 interacts with Ku80 and is recruited to sites of damage in a catalytic activity dependent manner. (A) Summary of mass spectrometry analysis of immunoprecipitate with an anti-GFP antibody from GFP-UCHL3 expressing cells. The hits obtained from GFP-UCHL3 over-expressing cells, but not detected with GFP-expressing cells (control) are shown with the number of peptide matches. Only proteins related to DNA damage responses are listed. (B , C) U2OS cells were transfected with a plasmid expressing GFP-UCHL3 (B) or GFP-Ku80 (C) . The soluble fraction was subjected to immunoprecipitation with an anti-GFP antibody with or without EtBr followed by immunoblotting with the indicated antibodies. Transfection with the plasmid expressing GFP was used as a negative control. (D) The soluble fraction of U2OS cells was subjected to immunoprecipitation with an anti-UCHL3 antibody (Ab) or rabbit IgG (IgG) followed by immunoblotting with the indicated antibodies. (E) .

    Journal: Scientific Reports

    Article Title: The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage

    doi: 10.1038/s41598-018-36235-0

    Figure Lengend Snippet: UCHL3 interacts with Ku80 and is recruited to sites of damage in a catalytic activity dependent manner. (A) Summary of mass spectrometry analysis of immunoprecipitate with an anti-GFP antibody from GFP-UCHL3 expressing cells. The hits obtained from GFP-UCHL3 over-expressing cells, but not detected with GFP-expressing cells (control) are shown with the number of peptide matches. Only proteins related to DNA damage responses are listed. (B , C) U2OS cells were transfected with a plasmid expressing GFP-UCHL3 (B) or GFP-Ku80 (C) . The soluble fraction was subjected to immunoprecipitation with an anti-GFP antibody with or without EtBr followed by immunoblotting with the indicated antibodies. Transfection with the plasmid expressing GFP was used as a negative control. (D) The soluble fraction of U2OS cells was subjected to immunoprecipitation with an anti-UCHL3 antibody (Ab) or rabbit IgG (IgG) followed by immunoblotting with the indicated antibodies. (E) .

    Article Snippet: U2OS stably expressing the HR reporter direct repeat-GFP, EJ2-GFP reporter and U2OS UCHL3 knock out cell lines were cultured in identical media with 1 μg/ml puromycin (InvivoGen) instead of Geneticin.

    Techniques: Activity Assay, Mass Spectrometry, Expressing, Transfection, Plasmid Preparation, Immunoprecipitation, Negative Control

    UCHL3 phosphorylation requiring its catalytic activity and downstream NHEJ factors regulates UCHL3 stability. (A) U2OS cells transfected with a plasmid expressing FLAG-UCHL3 were treated with phleomycin. Immunoprecipitation with an anti-FLAG antibody was carried out, followed by immunoblotting analysis with the indicated antibodies. Transfection with the plasmid coding FLAG was used as a negative control. (B) U2OS cells transfected with a plasmid expressing either FLAG-UCHL3 (wild-type: WT) or catalytically inactive mutant (C95A) were treated with phleomycin or mock treated. Immunoprecipitation with an anti-FLAG antibody was carried out, followed by immunoblotting analysis with the indicated antibodies. Transfection with the plasmid coding FLAG was used as a negative control. (C) U2OS cells transfected with a plasmid expressing either FLAG or FLAG-UCHL3 were treated with phleomycin for 1 hour and further cultured for the indicated time periods after removal of phleomycin. Cells were processed for immunoprecipitation with anti-FLAG antibody followed by immunoblotting analyses with the indicated antibodies. (D) U2OS cells transfected with the indicated siRNAs were further transfected with a plasmid expressing either GFP-UCHL3 or GFP. Following phleomycin treatment, cell extracts were subjected to immunoprecipitation with an anti-GFP antibody and ensuing immunoblotting analysis with the indicated antibodies. The plasmid expressing GFP was used as a negative control. (E) .

    Journal: Scientific Reports

    Article Title: The deubiquitylating enzyme UCHL3 regulates Ku80 retention at sites of DNA damage

    doi: 10.1038/s41598-018-36235-0

    Figure Lengend Snippet: UCHL3 phosphorylation requiring its catalytic activity and downstream NHEJ factors regulates UCHL3 stability. (A) U2OS cells transfected with a plasmid expressing FLAG-UCHL3 were treated with phleomycin. Immunoprecipitation with an anti-FLAG antibody was carried out, followed by immunoblotting analysis with the indicated antibodies. Transfection with the plasmid coding FLAG was used as a negative control. (B) U2OS cells transfected with a plasmid expressing either FLAG-UCHL3 (wild-type: WT) or catalytically inactive mutant (C95A) were treated with phleomycin or mock treated. Immunoprecipitation with an anti-FLAG antibody was carried out, followed by immunoblotting analysis with the indicated antibodies. Transfection with the plasmid coding FLAG was used as a negative control. (C) U2OS cells transfected with a plasmid expressing either FLAG or FLAG-UCHL3 were treated with phleomycin for 1 hour and further cultured for the indicated time periods after removal of phleomycin. Cells were processed for immunoprecipitation with anti-FLAG antibody followed by immunoblotting analyses with the indicated antibodies. (D) U2OS cells transfected with the indicated siRNAs were further transfected with a plasmid expressing either GFP-UCHL3 or GFP. Following phleomycin treatment, cell extracts were subjected to immunoprecipitation with an anti-GFP antibody and ensuing immunoblotting analysis with the indicated antibodies. The plasmid expressing GFP was used as a negative control. (E) .

    Article Snippet: U2OS stably expressing the HR reporter direct repeat-GFP, EJ2-GFP reporter and U2OS UCHL3 knock out cell lines were cultured in identical media with 1 μg/ml puromycin (InvivoGen) instead of Geneticin.

    Techniques: Activity Assay, Non-Homologous End Joining, Transfection, Plasmid Preparation, Expressing, Immunoprecipitation, Negative Control, Mutagenesis, Cell Culture

    Autophagosome and lysosome levels in control and Hsp90 inhibitor-treated cells. a. Stable clones of RKO expressing GFP (negative control) or GFP-LC3 (NEG-siRNA or HSF1siRNA transfected) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Fluorescent

    Journal: Biochemical pharmacology

    Article Title: Heat Shock Factor 1 Confers Resistance to Hsp90 Inhibitors through p62/SQSTM1 Expression and Promotion of Autophagic Flux

    doi: 10.1016/j.bcp.2013.11.014

    Figure Lengend Snippet: Autophagosome and lysosome levels in control and Hsp90 inhibitor-treated cells. a. Stable clones of RKO expressing GFP (negative control) or GFP-LC3 (NEG-siRNA or HSF1siRNA transfected) were treated with geldanamycin or 17-AAG (250 nM) for 8 h. Fluorescent

    Article Snippet: For visualizing autophagosomes, an expression construct containing the human LC3B gene fused at 5’ end to the GFP gene (pSELECT-GFP-LC3) and control plasmid expressing GFP alone (pSELECT-NGFP-zeo) were both obtained from InvivoGen.

    Techniques: Clone Assay, Expressing, Negative Control, Transfection