Journal: Journal of Virology

Article Title: Unique Evolution of Antiviral Tetherin in Bats

doi: 10.1128/jvi.01152-22

Figure Lengend Snippet: Pteropus alecto tetherin restricts the release of Ebola and Marburg virus-like particles (VLPs). P. alecto tetherin isoforms A (PA), B (PB), and C (PC), co-transfected with an (A) Ebola or (B) Marburg virus construct. Mammalian HEK293T cells were co-transfected with 200 ng of Ebola or Marburg virus plasmid expression construct encoding a VP40-eGFP protein which generates VLPs, and 0 to 200 ng of the tetherin plasmid expression vector. VLPs were harvested at 48 h and concentrated by ultracentrifugation through a sucrose gradient. VLP and cell lysates were subjected to SDS-PAGE and Western blot analysis. VLPs were detected with a mouse anti-GFP 4B10 primary antibody and a goat anti-mouse Alexa Fluor 680 fluorophore-conjugated fluorescent secondary antibody. Representative Western blots are shown. The extent of VLP restriction was quantitated by densitometric analysis of Western blots comparing the relative ratios of VLPs present in the viral lysates and cell lysates from n = 5 independent assays. Error bars represent the standard deviation. A nonparametric Wilcoxon rank-sum test was performed to calculate the statistical significance of the restriction of Ebola and Marburg VLPs in the 200 ng tetherin isoform A, B, and C treatment groups; P < 0.01 in all cases. SN, cell culture supernatant; CL, cell culture lysate.

Article Snippet: For Ebola and Marburg VLPs, the primary antibody binding was directed against eGFP using a solution containing a 1/4,000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, Danvers, MA).

Techniques: Transfection, Construct, Plasmid Preparation, Expressing, SDS Page, Western Blot, Standard Deviation, Cell Culture

Journal: Science Advances

Article Title: Efficacy and specificity of inhibitors of BCL-2 family protein interactions assessed by affinity measurements in live cells

doi: 10.1126/sciadv.abm7375

Figure Lengend Snippet: List of key resources. List of source and identifier for reagents and resources used here. Links to all uploaded data, software, and custom-written scripts are listed. NA, not applicable; PBS, phosphate-buffered saline; 3D, three dimensions; NMR, nuclear magnetic resonance; PDB, Protein Data Bank; HEK, human embryonic kidney.

Article Snippet: GFP (4B10) Mouse mAb , Cell Signaling Technologies , Catalog no. 2955S, RRID:AB_1196614.

Techniques: Software, Nuclear Magnetic Resonance, Labeling, Produced, Purification, Recombinant, Plasmid Preparation, Transfection, Western Blot, Expressing, Stable Transfection, Binding Assay, Double Knockout, Microscopy, High Content Screening

Journal: Cell reports

Article Title: Interplay between protein acetylation and ubiquitination controls MCL1 protein stability

doi: 10.1016/j.celrep.2021.109988

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti-GFP (4B10) , Cell Signaling Technology , Cat# 2955, RRID: AB_1196614.

Techniques: Purification, Recombinant, Mutagenesis, Cell Fractionation, Mass Spectrometry, Western Blot, CRISPR, Software