gfap  (Boster Bio)


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    Name:
    Anti GFAP Rabbit Monoclonal Antibody
    Description:

    Catalog Number:
    M00213-1
    Price:
    299.0
    Category:
    Primary Antibodies
    Reactivity:
    Human Mouse Rat
    Applications:
    IP, IF, IHC, ICC, WB
    Immunogen:
    A synthesized peptide derived from human GFAP
    Host:
    Rabbit
    Buy from Supplier


    Structured Review

    Boster Bio gfap
    Anti GFAP Rabbit Monoclonal Antibody

    https://www.bioz.com/result/gfap/product/Boster Bio
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfap - by Bioz Stars, 2021-07
    92/100 stars

    Images

    1) Product Images from "Region-Restrict Astrocytes Exhibit Heterogeneous Susceptibility to Neuronal Reprogramming"

    Article Title: Region-Restrict Astrocytes Exhibit Heterogeneous Susceptibility to Neuronal Reprogramming

    Journal: Stem Cell Reports

    doi: 10.1016/j.stemcr.2018.12.017

    Astrocyte Heterogeneity in Gene Expression and Proliferation (A) Immunohistochemical analysis of GFAP and Aldoc in astrocytes from adult mouse cortex, cerebellum, and spinal cord (n = 3; cells = 1,500–2,000 for each condition). (B) The heterogeneous gene expression in cortex-, cerebellum-, and spinal cord-derived cultured postnatal or adult astrocytes determined by real-time PCR (a representative result from three independent experiments). (C) The heterogeneous cell proliferation of regional postnatal astrocytes (n = 3; cells = 1,000–1,500 for each condition). ∗ p
    Figure Legend Snippet: Astrocyte Heterogeneity in Gene Expression and Proliferation (A) Immunohistochemical analysis of GFAP and Aldoc in astrocytes from adult mouse cortex, cerebellum, and spinal cord (n = 3; cells = 1,500–2,000 for each condition). (B) The heterogeneous gene expression in cortex-, cerebellum-, and spinal cord-derived cultured postnatal or adult astrocytes determined by real-time PCR (a representative result from three independent experiments). (C) The heterogeneous cell proliferation of regional postnatal astrocytes (n = 3; cells = 1,000–1,500 for each condition). ∗ p

    Techniques Used: Expressing, Immunohistochemistry, Derivative Assay, Cell Culture, Real-time Polymerase Chain Reaction

    2) Product Images from "Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice"

    Article Title: Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.029801

    Activation of L1-mediated intracellular signaling cascades at 6 weeks after SCI in mice treated with trimebutine or honokiol. Western blot analysis of L1 (A), phosphorylation levels of Akt1 (B), Erk1/2 (C), CK2α (D) and mTOR (E), protein levels of PTEN (F), p53 (G) and GFAP (H), and Bcl-2/Bax ratio (I) of mice treated with vehicle control (VC), trimebutine (TMB) or honokiol (HNK) at 5 mm rostral to the lesion center, the lesion center, and 5 mm caudal to the lesion center. * P
    Figure Legend Snippet: Activation of L1-mediated intracellular signaling cascades at 6 weeks after SCI in mice treated with trimebutine or honokiol. Western blot analysis of L1 (A), phosphorylation levels of Akt1 (B), Erk1/2 (C), CK2α (D) and mTOR (E), protein levels of PTEN (F), p53 (G) and GFAP (H), and Bcl-2/Bax ratio (I) of mice treated with vehicle control (VC), trimebutine (TMB) or honokiol (HNK) at 5 mm rostral to the lesion center, the lesion center, and 5 mm caudal to the lesion center. * P

    Techniques Used: Activation Assay, Mouse Assay, Western Blot

    GFAP, βIII-tubulin and NF200 immunoreactivity at 6 weeks after SCI in mice treated with trimebutine or honokiol. (A-I) Representative micrographs of GFAP (A,E,F), βIII-tubulin (C,E) and NF200 (F) staining, and quantitative analysis of intensity of GFAP (B,G), βIII-tubulin (D,H) and NF200 (I) immunoreactivity. * P
    Figure Legend Snippet: GFAP, βIII-tubulin and NF200 immunoreactivity at 6 weeks after SCI in mice treated with trimebutine or honokiol. (A-I) Representative micrographs of GFAP (A,E,F), βIII-tubulin (C,E) and NF200 (F) staining, and quantitative analysis of intensity of GFAP (B,G), βIII-tubulin (D,H) and NF200 (I) immunoreactivity. * P

    Techniques Used: Mouse Assay, Staining

    3) Product Images from "Let-7f promotes the differentiation of neural stem cells in rats"

    Article Title: Let-7f promotes the differentiation of neural stem cells in rats

    Journal: American Journal of Translational Research

    doi:

    In vitro differentiation of NSCs. Immunofluorescence staining of adherent neurospheres showed positive staining for Nestin (A). Immunofluorescence staining of differentiated NSCs showed positive staining for astrocyte marker GFAP (B) and neuron marker Tuj1 (C). Cell nuclei were counterstained with DAPI (blue). DAPI, 4’,6-diamidino-2-phenylindole.
    Figure Legend Snippet: In vitro differentiation of NSCs. Immunofluorescence staining of adherent neurospheres showed positive staining for Nestin (A). Immunofluorescence staining of differentiated NSCs showed positive staining for astrocyte marker GFAP (B) and neuron marker Tuj1 (C). Cell nuclei were counterstained with DAPI (blue). DAPI, 4’,6-diamidino-2-phenylindole.

    Techniques Used: In Vitro, Immunofluorescence, Staining, Marker

    The increase of let-7f expression level is associated with differentiation of NSCs in vitro . A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 following the induction of NSCs on days 0, 3, 5 and 7, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected in NSCs subject to differentiation on days 0, 3, 5 and 7 by qRT-PCR. Data shown as mean ± SD. * P
    Figure Legend Snippet: The increase of let-7f expression level is associated with differentiation of NSCs in vitro . A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 following the induction of NSCs on days 0, 3, 5 and 7, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected in NSCs subject to differentiation on days 0, 3, 5 and 7 by qRT-PCR. Data shown as mean ± SD. * P

    Techniques Used: Expressing, In Vitro, Western Blot, Quantitative RT-PCR

    The increase of let-7f expression level is age-dependent. A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 in brains of rats at three different ages (Postnatal Day 1 (P1), P8 and P14), with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected by qRT-PCR in brains of P1, P8 and P14 rats. Data shown as mean ± SD. * P
    Figure Legend Snippet: The increase of let-7f expression level is age-dependent. A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 in brains of rats at three different ages (Postnatal Day 1 (P1), P8 and P14), with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected by qRT-PCR in brains of P1, P8 and P14 rats. Data shown as mean ± SD. * P

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR

    Leverage of let-7 expression level has an impact on the differentiation of NSCs. A. Expression of EGFP was observed in transduced cells under fluorescence microscope. Uninfected cells were used as controls. B. Representative western blotting showing the expressions of Nestin, GFAP and Tuj1 in uninfected and infected differentiated NSCs, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. Data shown as mean ± SD. * P
    Figure Legend Snippet: Leverage of let-7 expression level has an impact on the differentiation of NSCs. A. Expression of EGFP was observed in transduced cells under fluorescence microscope. Uninfected cells were used as controls. B. Representative western blotting showing the expressions of Nestin, GFAP and Tuj1 in uninfected and infected differentiated NSCs, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. Data shown as mean ± SD. * P

    Techniques Used: Expressing, Fluorescence, Microscopy, Western Blot, Infection

    4) Product Images from "The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects"

    Article Title: The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects

    Journal: Nutrition Journal

    doi: 10.1186/s12937-016-0212-7

    a Neurosphere exhibited adherent growth after several hours of incubation. Extensive thin, elongated projections were observed, connecting adjacent neurospheres into a complex network (100x); b Immunocytochemical staining showing primary NSCs with Nestin-positive cytoplasm and Nestin-negative nuclei (200x); c Small, round/oval/triangle NSE-positive neurons with thin and long projections were observed at day 5 since the differentiation induction (200x). Synaptic connections have been established between neurons ( red arrow ); and d large, irregular-shaped GFAP-positive astrocytes with multiple short and flat projections were observed at day 5 since the differentiation induction (200x)
    Figure Legend Snippet: a Neurosphere exhibited adherent growth after several hours of incubation. Extensive thin, elongated projections were observed, connecting adjacent neurospheres into a complex network (100x); b Immunocytochemical staining showing primary NSCs with Nestin-positive cytoplasm and Nestin-negative nuclei (200x); c Small, round/oval/triangle NSE-positive neurons with thin and long projections were observed at day 5 since the differentiation induction (200x). Synaptic connections have been established between neurons ( red arrow ); and d large, irregular-shaped GFAP-positive astrocytes with multiple short and flat projections were observed at day 5 since the differentiation induction (200x)

    Techniques Used: Incubation, Staining

    5) Product Images from "Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice"

    Article Title: Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice

    Journal: Disease Models & Mechanisms

    doi: 10.1242/dmm.029801

    Activation of L1-mediated intracellular signaling cascades at 6 weeks after SCI in mice treated with trimebutine or honokiol. Western blot analysis of L1 (A), phosphorylation levels of Akt1 (B), Erk1/2 (C), CK2α (D) and mTOR (E), protein levels of PTEN (F), p53 (G) and GFAP (H), and Bcl-2/Bax ratio (I) of mice treated with vehicle control (VC), trimebutine (TMB) or honokiol (HNK) at 5 mm rostral to the lesion center, the lesion center, and 5 mm caudal to the lesion center. * P
    Figure Legend Snippet: Activation of L1-mediated intracellular signaling cascades at 6 weeks after SCI in mice treated with trimebutine or honokiol. Western blot analysis of L1 (A), phosphorylation levels of Akt1 (B), Erk1/2 (C), CK2α (D) and mTOR (E), protein levels of PTEN (F), p53 (G) and GFAP (H), and Bcl-2/Bax ratio (I) of mice treated with vehicle control (VC), trimebutine (TMB) or honokiol (HNK) at 5 mm rostral to the lesion center, the lesion center, and 5 mm caudal to the lesion center. * P

    Techniques Used: Activation Assay, Mouse Assay, Western Blot

    GFAP, βIII-tubulin and NF200 immunoreactivity at 6 weeks after SCI in mice treated with trimebutine or honokiol. (A-I) Representative micrographs of GFAP (A,E,F), βIII-tubulin (C,E) and NF200 (F) staining, and quantitative analysis of intensity of GFAP (B,G), βIII-tubulin (D,H) and NF200 (I) immunoreactivity. * P
    Figure Legend Snippet: GFAP, βIII-tubulin and NF200 immunoreactivity at 6 weeks after SCI in mice treated with trimebutine or honokiol. (A-I) Representative micrographs of GFAP (A,E,F), βIII-tubulin (C,E) and NF200 (F) staining, and quantitative analysis of intensity of GFAP (B,G), βIII-tubulin (D,H) and NF200 (I) immunoreactivity. * P

    Techniques Used: Mouse Assay, Staining

    6) Product Images from "Let-7f promotes the differentiation of neural stem cells in rats"

    Article Title: Let-7f promotes the differentiation of neural stem cells in rats

    Journal: American Journal of Translational Research

    doi:

    In vitro differentiation of NSCs. Immunofluorescence staining of adherent neurospheres showed positive staining for Nestin (A). Immunofluorescence staining of differentiated NSCs showed positive staining for astrocyte marker GFAP (B) and neuron marker Tuj1 (C). Cell nuclei were counterstained with DAPI (blue). DAPI, 4’,6-diamidino-2-phenylindole.
    Figure Legend Snippet: In vitro differentiation of NSCs. Immunofluorescence staining of adherent neurospheres showed positive staining for Nestin (A). Immunofluorescence staining of differentiated NSCs showed positive staining for astrocyte marker GFAP (B) and neuron marker Tuj1 (C). Cell nuclei were counterstained with DAPI (blue). DAPI, 4’,6-diamidino-2-phenylindole.

    Techniques Used: In Vitro, Immunofluorescence, Staining, Marker

    The increase of let-7f expression level is associated with differentiation of NSCs in vitro . A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 following the induction of NSCs on days 0, 3, 5 and 7, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected in NSCs subject to differentiation on days 0, 3, 5 and 7 by qRT-PCR. Data shown as mean ± SD. * P
    Figure Legend Snippet: The increase of let-7f expression level is associated with differentiation of NSCs in vitro . A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 following the induction of NSCs on days 0, 3, 5 and 7, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected in NSCs subject to differentiation on days 0, 3, 5 and 7 by qRT-PCR. Data shown as mean ± SD. * P

    Techniques Used: Expressing, In Vitro, Western Blot, Quantitative RT-PCR

    The increase of let-7f expression level is age-dependent. A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 in brains of rats at three different ages (Postnatal Day 1 (P1), P8 and P14), with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected by qRT-PCR in brains of P1, P8 and P14 rats. Data shown as mean ± SD. * P
    Figure Legend Snippet: The increase of let-7f expression level is age-dependent. A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 in brains of rats at three different ages (Postnatal Day 1 (P1), P8 and P14), with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected by qRT-PCR in brains of P1, P8 and P14 rats. Data shown as mean ± SD. * P

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR

    Leverage of let-7 expression level has an impact on the differentiation of NSCs. A. Expression of EGFP was observed in transduced cells under fluorescence microscope. Uninfected cells were used as controls. B. Representative western blotting showing the expressions of Nestin, GFAP and Tuj1 in uninfected and infected differentiated NSCs, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. Data shown as mean ± SD. * P
    Figure Legend Snippet: Leverage of let-7 expression level has an impact on the differentiation of NSCs. A. Expression of EGFP was observed in transduced cells under fluorescence microscope. Uninfected cells were used as controls. B. Representative western blotting showing the expressions of Nestin, GFAP and Tuj1 in uninfected and infected differentiated NSCs, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. Data shown as mean ± SD. * P

    Techniques Used: Expressing, Fluorescence, Microscopy, Western Blot, Infection

    7) Product Images from "Gabrb2-knockout mice displayed schizophrenia-like and comorbid phenotypes with interneuron–astrocyte–microglia dysregulation"

    Article Title: Gabrb2-knockout mice displayed schizophrenia-like and comorbid phenotypes with interneuron–astrocyte–microglia dysregulation

    Journal: Translational Psychiatry

    doi: 10.1038/s41398-018-0176-9

    Neuron and astrocyte immunohistochemical analysis and mRNA expression of GABA A receptor subunits. a Outline of sagittal section of mouse brain, showing the brain regions analyzed using immunohistochemical staining. b – i Coronal sections stained through fluorescence-labeling of: b NeuN in anterior cingulate cortex (ACC); c NeuN in hippocampus (HC); d DCX in dentate gyrus (DG); e parvalbumin (PV) in ACC; f PV in piriform cortex (PC); g PV in DG; h GFAP in CA1 region; and i GFAP in DG. The DG, CA1, and CA3 regions in hippocampus are indicated in part ( c ). NeuN and PV immunofluorescence are displayed in green (scale bar = 300 μm), and that of DCX and GFAP and DCX displayed in red (scale bar = 120 μm). The plots show immunofluorescence densities for WT (green dots) and KO (red dots) estimated in terms of average area optical density (in parts b , c , e , h , and i ), or in terms of the number of immunoreactive cells (in parts d , f , g ); in each instance, five randomly selected images from each of five WT or KO male mice were examined. The levels of mRNA expression for 13 different GABA A receptor subunits in WT and KO mouse cerebrum ( j ) and cerebellum ( k ) were measured using quantitative RT-PCR, and the mRNA levels in KO mice were normalized to the average expression level in WT ( n . Statistical analysis was performed using unpaired t -test for immunohistochemical staining and one-way ANOVA with Newman–Keuls post-hoc test for mRNA quantitation. Average y values ± SEM in the different plots are represented by horizontal bars; WT is represented by green dots and KO by red dots. N.D. represents non-detectable; * p
    Figure Legend Snippet: Neuron and astrocyte immunohistochemical analysis and mRNA expression of GABA A receptor subunits. a Outline of sagittal section of mouse brain, showing the brain regions analyzed using immunohistochemical staining. b – i Coronal sections stained through fluorescence-labeling of: b NeuN in anterior cingulate cortex (ACC); c NeuN in hippocampus (HC); d DCX in dentate gyrus (DG); e parvalbumin (PV) in ACC; f PV in piriform cortex (PC); g PV in DG; h GFAP in CA1 region; and i GFAP in DG. The DG, CA1, and CA3 regions in hippocampus are indicated in part ( c ). NeuN and PV immunofluorescence are displayed in green (scale bar = 300 μm), and that of DCX and GFAP and DCX displayed in red (scale bar = 120 μm). The plots show immunofluorescence densities for WT (green dots) and KO (red dots) estimated in terms of average area optical density (in parts b , c , e , h , and i ), or in terms of the number of immunoreactive cells (in parts d , f , g ); in each instance, five randomly selected images from each of five WT or KO male mice were examined. The levels of mRNA expression for 13 different GABA A receptor subunits in WT and KO mouse cerebrum ( j ) and cerebellum ( k ) were measured using quantitative RT-PCR, and the mRNA levels in KO mice were normalized to the average expression level in WT ( n . Statistical analysis was performed using unpaired t -test for immunohistochemical staining and one-way ANOVA with Newman–Keuls post-hoc test for mRNA quantitation. Average y values ± SEM in the different plots are represented by horizontal bars; WT is represented by green dots and KO by red dots. N.D. represents non-detectable; * p

    Techniques Used: Immunohistochemistry, Expressing, Staining, Fluorescence, Labeling, Immunofluorescence, Mouse Assay, Quantitative RT-PCR, Quantitation Assay

    8) Product Images from "Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules"

    Article Title: Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

    Journal: Journal of Translational Medicine

    doi: 10.1186/s12967-015-0727-2

    β -Elemene regulation of stemness-, differentiation- and EMT-related effectors in vivo. a Western blot assays were performed on the aforementioned glioblastoma xenografts in the β -elemene- and NaCl-treated groups. Expression levels of CD133, ABCG2, N-cadherin and β -catenin were significantly downregulated and expression levels of E-cadherin, GFAP, Notch1 and SHH were upregulated by β -elemene. Interestingly, the expression level of vimentin in the β -elemene-treated group was significantly lower than that in the NaCl-treated group; this result was opposite that for the in vitro procedure. b The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p
    Figure Legend Snippet: β -Elemene regulation of stemness-, differentiation- and EMT-related effectors in vivo. a Western blot assays were performed on the aforementioned glioblastoma xenografts in the β -elemene- and NaCl-treated groups. Expression levels of CD133, ABCG2, N-cadherin and β -catenin were significantly downregulated and expression levels of E-cadherin, GFAP, Notch1 and SHH were upregulated by β -elemene. Interestingly, the expression level of vimentin in the β -elemene-treated group was significantly lower than that in the NaCl-treated group; this result was opposite that for the in vitro procedure. b The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Techniques Used: In Vivo, Western Blot, Expressing, In Vitro

    β -Elemene regulated the expression of stemness markers and differentiation-related effectors in glioblastoma cells. a CD133 + and ABCG2 + cells were sparsely distributed throughout both G1 and G2 tissues, and both CD133 and ABCG2 were localized to both the cytoplasm and cytomembrane. The expression of GFAP was higher in the G1 tissue than in the G2 tissue. b β -Elemene decreased the expression levels of CD133 and ABCG2 and increased the expression levels of GFAP, Notch1 and SHH in a dose-dependent manner. c The results of ( b ) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p
    Figure Legend Snippet: β -Elemene regulated the expression of stemness markers and differentiation-related effectors in glioblastoma cells. a CD133 + and ABCG2 + cells were sparsely distributed throughout both G1 and G2 tissues, and both CD133 and ABCG2 were localized to both the cytoplasm and cytomembrane. The expression of GFAP was higher in the G1 tissue than in the G2 tissue. b β -Elemene decreased the expression levels of CD133 and ABCG2 and increased the expression levels of GFAP, Notch1 and SHH in a dose-dependent manner. c The results of ( b ) were semi-quantitatively estimated using Gel-Pro Analyzer 4.0 software and are illustrated graphically. The results are representative of three independent experiments, and the values are presented as the mean ± SD (* p

    Techniques Used: Expressing, Software

    9) Product Images from "Gabrb2-knockout mice displayed schizophrenia-like and comorbid phenotypes with interneuron–astrocyte–microglia dysregulation"

    Article Title: Gabrb2-knockout mice displayed schizophrenia-like and comorbid phenotypes with interneuron–astrocyte–microglia dysregulation

    Journal: Translational Psychiatry

    doi: 10.1038/s41398-018-0176-9

    Neuron and astrocyte immunohistochemical analysis and mRNA expression of GABA A receptor subunits. a Outline of sagittal section of mouse brain, showing the brain regions analyzed using immunohistochemical staining. b – i Coronal sections stained through fluorescence-labeling of: b NeuN in anterior cingulate cortex (ACC); c NeuN in hippocampus (HC); d DCX in dentate gyrus (DG); e parvalbumin (PV) in ACC; f PV in piriform cortex (PC); g PV in DG; h GFAP in CA1 region; and i GFAP in DG. The DG, CA1, and CA3 regions in hippocampus are indicated in part ( c ). NeuN and PV immunofluorescence are displayed in green (scale bar = 300 μm), and that of DCX and GFAP and DCX displayed in red (scale bar = 120 μm). The plots show immunofluorescence densities for WT (green dots) and KO (red dots) estimated in terms of average area optical density (in parts b , c , e , h , and i ), or in terms of the number of immunoreactive cells (in parts d , f , g ); in each instance, five randomly selected images from each of five WT or KO male mice were examined. The levels of mRNA expression for 13 different GABA A receptor subunits in WT and KO mouse cerebrum ( j ) and cerebellum ( k ) were measured using quantitative RT-PCR, and the mRNA levels in KO mice were normalized to the average expression level in WT ( n = 10 in each group). The measured expression levels in WT, KO as well as HT brains are given in Supplementary Table S4 . Statistical analysis was performed using unpaired t -test for immunohistochemical staining and one-way ANOVA with Newman–Keuls post-hoc test for mRNA quantitation. Average y values ± SEM in the different plots are represented by horizontal bars; WT is represented by green dots and KO by red dots. N.D. represents non-detectable; * p
    Figure Legend Snippet: Neuron and astrocyte immunohistochemical analysis and mRNA expression of GABA A receptor subunits. a Outline of sagittal section of mouse brain, showing the brain regions analyzed using immunohistochemical staining. b – i Coronal sections stained through fluorescence-labeling of: b NeuN in anterior cingulate cortex (ACC); c NeuN in hippocampus (HC); d DCX in dentate gyrus (DG); e parvalbumin (PV) in ACC; f PV in piriform cortex (PC); g PV in DG; h GFAP in CA1 region; and i GFAP in DG. The DG, CA1, and CA3 regions in hippocampus are indicated in part ( c ). NeuN and PV immunofluorescence are displayed in green (scale bar = 300 μm), and that of DCX and GFAP and DCX displayed in red (scale bar = 120 μm). The plots show immunofluorescence densities for WT (green dots) and KO (red dots) estimated in terms of average area optical density (in parts b , c , e , h , and i ), or in terms of the number of immunoreactive cells (in parts d , f , g ); in each instance, five randomly selected images from each of five WT or KO male mice were examined. The levels of mRNA expression for 13 different GABA A receptor subunits in WT and KO mouse cerebrum ( j ) and cerebellum ( k ) were measured using quantitative RT-PCR, and the mRNA levels in KO mice were normalized to the average expression level in WT ( n = 10 in each group). The measured expression levels in WT, KO as well as HT brains are given in Supplementary Table S4 . Statistical analysis was performed using unpaired t -test for immunohistochemical staining and one-way ANOVA with Newman–Keuls post-hoc test for mRNA quantitation. Average y values ± SEM in the different plots are represented by horizontal bars; WT is represented by green dots and KO by red dots. N.D. represents non-detectable; * p

    Techniques Used: Immunohistochemistry, Expressing, Staining, Fluorescence, Labeling, Immunofluorescence, Mouse Assay, Quantitative RT-PCR, Quantitation Assay

    Related Articles

    Incubation:

    Article Title: Clobetasol propionate enhances neural stem cell and oligodendrocyte differentiation
    Article Snippet: Cells were washed three times with PBS and fixed with 4% paraformaldehyde at 4°C for 12 h. Subsequently, cells were blocked with 0.1% Triton X-100 in PBS supplemented with 3% bovine serum albumin (Beijing Solarbio Science & Technology Co., Ltd.) at 37°C for 45 min. .. Cells were incubated with the following primary antibodies: Anti-growth associated protein 43 (GAP-43; rabbit polyclonal; 1:200; cat. no. PA-1037), anti-myelin basic protein (MBP; rabbit polyclonal; 1:200; cat. no. BA0094), anti-nestin (1:200; cat. no. PB0920), anti-glial fibrillary acidic protein (GFAP; mouse monoclonal; 1:200; cat. no. PB0046; all Boster Biological Technology), anti-SHH (1:200; rabbit monoclonal; cat. no. ab53281; Abcam), anti-phosphorylated adenosine 5-monophosphate (p-AMP)-activated protein kinase (p-AMPK; rabbit polyclonal; 1:200; cat. no. sc-25792) and anti-phosphorylated mitogen-activated protein kinase (p-ERK; rabbit polyclonal; 1:200; cat. no. sc-514302; both Santa Cruz Biotechnology, Inc.) for 12 h at 4°C. .. Following primary antibody incubation, cells were washed three times with PBS and then incubated with secondary antibodies, Cy3-labeled goat anti-mouse/rabbit IgG (1:200; cat. no. C5838/C2821 Sigma-Aldrich; Merck KGaA) and Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200; cat. no. SAB4600388; Sigma-Aldrich; Merck KGaA) for 3 h at room temperature.

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression
    Article Snippet: Afterwards, the cells were incubated in 0.25% Triton X-100 for 15 minutes and blocked in 5% bovine serum albumin for 30 minutes. .. The neurons were incubated with rabbit anti-NSE (Boster) and mouse anti-β-tubulin (Proteintech) antibodies, while the astrocytes were incubated with rabbit anti-GFAP (Boster) antibody at 4°C overnight. .. After washing with 0.01 M PBS, the cells were incubated with anti-rabbit FITC-conjugated secondary antibodies (Proteintech) at 37°C for 30 minutes.

    Article Title: A rat model for studying neural stem cell transplantation
    Article Snippet: Cells were fixed with 4% paraformaldehyde (PA) at 4 °C for 30 min. Nonspecific binding sites were blocked with 5% goat serum in PBS, and the samples were permeabilized with 0.1% Triton X-100 in PBS for 1 h at room temperature. .. The specimens were incubated overnight at 4 °C with the primary antibodies at the following dilutions: mouse anti-nestin 1:100 (BD Pharmingen, USA); mouse anti-NSE 1:10 (Chemicon, USA); rabbit anti-GFAP 1:100 (Boster, China). .. The secondary FITC-conjugated antibodies were diluted 1:100 (goat anti-mouse or rabbit, ZhongShan GoldenBridge, China) and incubated with cells for 1 h at 37 °C protected from light.

    Labeling:

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression
    Article Snippet: The cells were then stained with DAPI (Abcam, Cambridge, UK) and analyzed with a high content analysis system (PerkinElmer, Boston, MA, USA). .. Astrocytes and brain microvascular endothelial cells were labeled with rabbit anti-glial fibrillary acidic protein (GFAP) (1:100; Boster) and rabbit anti-PECAM-1/CD31 (1:100; Boster) antibodies. .. Neurons and astrocytes were separately seeded in a 96-well culture plate (PerkinElmer) until 60–80% confluency, and then washed with 0.01 M PBS and fixed with 4% paraformaldehyde for 30 minutes.

    Staining:

    Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
    Article Snippet: .. The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or rabbit anti-rats GFAP antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) at 37°C. .. After being washed with PBS, the FITC-labeled anti-mouse IgG and Texas Red-labeled anti-rabbit IgG (Sigma, St. Louis, MO, USA) were added to the tissue drop wise and then incubated in the dark for 60 min before incubation with 4′,6-diamidino-2-phenylindole (DAPI; Sigma, St. Louis, MO, USA) for 5 min to label cell nuclei.

    Marker:

    Article Title: In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats
    Article Snippet: .. The slices were stained alternatively for neuronal specific enolase (NSE, neuronal marker) or rabbit anti-rats glial fibrillary acidic protein (GFAP, a astrocyte marker), mouse anti-BrdU antibody (1:100), rabbit anti-rats NSE antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) or rabbit anti-rats GFAP antibody (1:200; Wuhan Boster Biological Technology, Ltd., China) at 37°C. .. After being washed with PBS, the FITC-labeled anti-mouse IgG and Texas Red-labeled anti-rabbit IgG (Sigma, St. Louis, MO, USA) were added to the tissue drop wise and then incubated in the dark for 60 min before incubation with 4′,6-diamidino-2-phenylindole (DAPI; Sigma, St. Louis, MO, USA) for 5 min to label cell nuclei.

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    Boster Bio mouse anti glial fibrillary acidic protein gfap
    Localization of <t>OLFM3</t> in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and <t>GFAP</t> (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.
    Mouse Anti Glial Fibrillary Acidic Protein Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio rabbit anti glial fibrillary acidic protein gfap
    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and <t>microvascular</t> endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express <t>GFAP</t> (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.
    Rabbit Anti Glial Fibrillary Acidic Protein Gfap, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Boster Bio rabbit anti gfap
    EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of <t>C3</t> in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), <t>GFAP</t> (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.
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    Localization of OLFM3 in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and GFAP (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Olfactomedin-3 Enhances Seizure Activity by Interacting With AMPA Receptors in Epilepsy Models

    doi: 10.3389/fcell.2020.00722

    Figure Lengend Snippet: Localization of OLFM3 in the human neocortex and in the mouse cortex and hippocampus. (A–C) OLFM3 (green) and MAP2 (red) were co-expressed (yellow) in the human neocortex (A) , mouse cortex (B) , and mouse hippocampus (C) , while no co-localization of OLFM3 and GFAP (red) was detected (D–F) . (G–I) Representative images show that OLFM3 expression (green) overlapped (yellow) with that of the excitatory postsynaptic marker PSD95 (red) but not the excitatory presynaptic marker vGlut1 (purple) in the human neocortex (G) and mouse cortex (H) and hippocampus (I) . DAPI (blue) indicates cell nuclei. White squares indicate positive cells, and the scale bar represents 20 or 50 μm.

    Article Snippet: The following primary antibodies were used: rabbit anti-OLFM3 (1:50, Proteintech, Wuhan, China), mouse anti-glial fibrillary acidic protein (GFAP) (1:50, Boster Bioengineering, Wuhan, China), guinea pig anti-microtubule-associated protein 2 (MAP2) (1:200, Sysy, Göttingen, Germany), guinea pig anti-vGlut1(1:200, Sysy, Göttingen, Germany), mouse anti-PSD95 (1:100, Abcam, United States), mouse anti-GluA1 (1:50, Santa Cruz Biotechnology, United States), mouse anti-GluA2 (1:100, Abcam, United States), Alexa Fluor 488-conjugated goat anti-rabbit IgG antibody (1:50, Zhongshan Golden Bridge, Inc., Beijing, China), Alexa Fluor 594-conjugated goat anti-mouse IgG antibody (1:200, Zhongshan Golden Bridge Inc., Beijing, China), and Alexa Fluor 633-conjugated goat anti-guinea pig IgG antibody (1:50, Abcam, United States).

    Techniques: Expressing, Marker

    Protein expression levels of nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) neuron-markers in nerve growth factor (NGF)- and basic fibroblast factor (bFGF)-transfected bone marrow mesenchymal stem cells (BMSCs) 72 h post-transfection. (A) Differentiated cells were stained with nestin, NSE and GFAP antibodies and counterstained with DAPI (blue). Scale bar=20 µ m. The NGF and bFGF co-transfected BMSCs exhibited increased protein expression of nestin, NSE and GFAP, as demonstrated by a marked increase in green fluorescence after 72 h, detected by fluorescence microscopy. (B) Western blotting detected the protein expression levels of neuron markers in the transfected BMSCs after 72 h. The expression levels of the neuron markers were higher in the co-transfected BMSCs group, as compared with the other groups. The untransfected BMSCs and plv-blank-transfected cells exhibited a slight expression of neuron markers. (C) Western blot analysis of the protein expression levels of nestin, NSE and GFAP in the untransfected BMSCs group, plv-blank-transfected BMSCs group, bFGF-transfected BMSCs group, NGF-transfected BMSCs group, and NGF and bFGF co-transfected BMSCs group. Data show the protein levels normalized to GAPDH, and are presented as the mean ± standard deviation (SD) (n=5). * P

    Journal: Molecular Medicine Reports

    Article Title: Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro

    doi: 10.3892/mmr.2015.4553

    Figure Lengend Snippet: Protein expression levels of nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) neuron-markers in nerve growth factor (NGF)- and basic fibroblast factor (bFGF)-transfected bone marrow mesenchymal stem cells (BMSCs) 72 h post-transfection. (A) Differentiated cells were stained with nestin, NSE and GFAP antibodies and counterstained with DAPI (blue). Scale bar=20 µ m. The NGF and bFGF co-transfected BMSCs exhibited increased protein expression of nestin, NSE and GFAP, as demonstrated by a marked increase in green fluorescence after 72 h, detected by fluorescence microscopy. (B) Western blotting detected the protein expression levels of neuron markers in the transfected BMSCs after 72 h. The expression levels of the neuron markers were higher in the co-transfected BMSCs group, as compared with the other groups. The untransfected BMSCs and plv-blank-transfected cells exhibited a slight expression of neuron markers. (C) Western blot analysis of the protein expression levels of nestin, NSE and GFAP in the untransfected BMSCs group, plv-blank-transfected BMSCs group, bFGF-transfected BMSCs group, NGF-transfected BMSCs group, and NGF and bFGF co-transfected BMSCs group. Data show the protein levels normalized to GAPDH, and are presented as the mean ± standard deviation (SD) (n=5). * P

    Article Snippet: The plates were incubated overnight with primary anti-nestin (1:200; Santa Cruz Biotechnology, Inc.), anti-glial fibrillary acidic protein (GFAP; 1:200; Wuhan Boster Biological Technology, Ltd., Wuhan, China), anti-neuron specific enolase (NSE; 1:200; Wuhan Boster Biological Technology, Ltd.), and anti-β-tubulin III (1:500; Sigma-Aldrich) in blocking solution for 2 h. The slides were washed with blocking solution three times for 10 min each time, and the plates were incubated for 2 h with secondary antibodies conjugated to Alexa Fluor 488 (2 µ g/ml, Invitrogen Life Technologies) in blocking solution.

    Techniques: Expressing, Transfection, Staining, Fluorescence, Microscopy, Western Blot, Standard Deviation

    Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Journal: Neural Regeneration Research

    Article Title: Structural and functional damage to the hippocampal neurovascular unit in diabetes-related depression

    doi: 10.4103/1673-5374.244794

    Figure Lengend Snippet: Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.

    Article Snippet: Astrocytes and brain microvascular endothelial cells were labeled with rabbit anti-glial fibrillary acidic protein (GFAP) (1:100; Boster) and rabbit anti-PECAM-1/CD31 (1:100; Boster) antibodies.

    Techniques: Light Microscopy

    EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of C3 in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Electro-acupuncture Alleviates METH Withdrawal-induced Spatial Memory Deficits by Restoring Astrocyte-drived Glutamate Uptake in dCA1

    doi: 10.1101/2020.05.20.106153

    Figure Lengend Snippet: EA rescued METH-related neurotoxicity via normalizing the function of astrocyte. A. Expression of C3 in the dCA1 during METH withdrawal. (A1), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of SAL and METH group mice. White arrows indicate representative cells which were showed at a higher power view in the white box. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, numbers of GFAP-positive cells per mm 2 in SAL and METH groups. (A2), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of SAL and METH groups. (A3), Top panels: immunohistochemistry staining for C3 (green), GFAP (red) and DAPI (blue) in the hippocampus of M+SEA and M+EA group mice. Bottom panels: left, the proportion of C3 and GFAP double-label cells; middle, the proportion of reactive astrocytes; right, number of GFAP-positive cells per mm 2 . (A4), Western blots (top) and quantification (bottom) of C3 and GFAP protein levels in the dCA1 of M+SEA and M+EA group. B, GLT-1, GLAST and GS expression levels in the dCA1 during METH withdrawal. (B1), Immunohistochemistry staining (left, GLT-1/GLAST/GS-green; GFAP-red and DAPI-blue) and colocalized ratio (right) for GLT-1, GLAST and GS in the hippocampus of SAL and METH group mice. (B2), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of SAL and METH group. (B3), Immunohistochemistry staining (left) and colocalized ratio (right) for GLT-1, GLAST and GS of M+SEA and M+EA group. (B4), Western blots (top) and quantification (bottom) of GLT-1, GLAST and GS protein levels in the dCA1 of M+SEA and M+EA group. Data are Mean ± S.E.M.

    Article Snippet: The following primary antibodies were used in these experiments: rat anti-C3 (1:200; abcam, UK), mouse anti-S100A10 (1:200; Thermo Fisher Scientific, USA), rabbit anti-GFAP (1:300; Boster, China), rat anti-GFAP(1:500, Thermo Fisher Scientific, USA), rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300; Thermo Fisher Scientific, USA), mouse anti-GS (1:500; abcam, UK), rabbit anti-c-Fos (1:2000; Cell Signaling Technology, USA), rabbit anti-SYP1 (Synaptotagmin-1, 1;500; Signalway Antibody, USA), mouse anti-VGLUT-1 (1;500; 1:200; abcam, UK), rabbit anti-NeuN (1:100; MERCK, Germany).

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Western Blot

    A1-like astrocytes showed decreased capacity of Glu uptake. A, Glu clearance in primary cultured astrocytes. (A1), Representative micrographs (left) and quantification (right) for GFAP staining in primary astrocytes. (A2), Top panels: immunohistochemistry micrographs for C3 (green), GFAP (red) and DAPI (blue) of control and A1 group. Bottom panels: left, ratio of C3 positive astrocytes; right, mRNA expression levels of C3. (A3), Ratio of Glu clearance after 1h application of Glu (100μM) in the control and A1 group. B, GLT-1, GLAST and GS expression in the primary cultured astrocytes. (B1) , Immunohistochemistry micrographs (left, GLT-1/GLAST/GS-red; C3-green and DAPI-blue) and quantification (right) for GLT-1, GS and GLAST of control and A1 group. (B2), Western blots (top) and quantification (bottom) of GLT-1, GS and GLAST protein levels of control and A1 group. Data are Mean ± S.E.M.

    Journal: bioRxiv

    Article Title: Electro-acupuncture Alleviates METH Withdrawal-induced Spatial Memory Deficits by Restoring Astrocyte-drived Glutamate Uptake in dCA1

    doi: 10.1101/2020.05.20.106153

    Figure Lengend Snippet: A1-like astrocytes showed decreased capacity of Glu uptake. A, Glu clearance in primary cultured astrocytes. (A1), Representative micrographs (left) and quantification (right) for GFAP staining in primary astrocytes. (A2), Top panels: immunohistochemistry micrographs for C3 (green), GFAP (red) and DAPI (blue) of control and A1 group. Bottom panels: left, ratio of C3 positive astrocytes; right, mRNA expression levels of C3. (A3), Ratio of Glu clearance after 1h application of Glu (100μM) in the control and A1 group. B, GLT-1, GLAST and GS expression in the primary cultured astrocytes. (B1) , Immunohistochemistry micrographs (left, GLT-1/GLAST/GS-red; C3-green and DAPI-blue) and quantification (right) for GLT-1, GS and GLAST of control and A1 group. (B2), Western blots (top) and quantification (bottom) of GLT-1, GS and GLAST protein levels of control and A1 group. Data are Mean ± S.E.M.

    Article Snippet: The following primary antibodies were used in these experiments: rat anti-C3 (1:200; abcam, UK), mouse anti-S100A10 (1:200; Thermo Fisher Scientific, USA), rabbit anti-GFAP (1:300; Boster, China), rat anti-GFAP(1:500, Thermo Fisher Scientific, USA), rabbit anti-GLT-1 (1:300; Cell Signaling Technology, USA), rabbit anti-GLAST (1:300; Thermo Fisher Scientific, USA), mouse anti-GS (1:500; abcam, UK), rabbit anti-c-Fos (1:2000; Cell Signaling Technology, USA), rabbit anti-SYP1 (Synaptotagmin-1, 1;500; Signalway Antibody, USA), mouse anti-VGLUT-1 (1;500; 1:200; abcam, UK), rabbit anti-NeuN (1:100; MERCK, Germany).

    Techniques: Cell Culture, Staining, Immunohistochemistry, Expressing, Western Blot