genus ruminococcus  (ATCC)

Journal: PLoS ONE

Article Title: Characterization of the Core Rumen Microbiome in Cattle during Transition from Forage to Concentrate as Well as during and after an Acidotic Challenge

doi: 10.1371/journal.pone.0083424

Figure Lengend Snippet: Effect of dietary treatment and digesta fraction on the percent of total enumerated eubacteria 16S rRNA genes of dominant rumen bacterial species using quantitative real-time PCR.

Article Snippet: Quantitative analysis was performed with the ABI PRISM 7700 Sequence Detection System (AB Applied Biosystems, Life Technologies Corp.), to quantify the relative abundance of 16S rRNA genes of several groups: Genus Ruminococcus , genus Prevotella , Selenomonas ruminantium , Fibrobacter succinogenes , Megasphaera elsdenii , and Streptococcus spp. were all measured as a percentage of total bacterial 16S rRNA using previously reported primers .

Techniques:

Journal: PLoS ONE

Article Title: Characterization of the Core Rumen Microbiome in Cattle during Transition from Forage to Concentrate as Well as during and after an Acidotic Challenge

doi: 10.1371/journal.pone.0083424

Figure Lengend Snippet: Percent abundance of taxa to the “Rumen Core Microbiome” and the dietary microbiomes for forage, mixed forage, high grain, acidotic challenge (4 h post-feeding and 12 h post-feeding) and recovery period shown with ± standard error of the mean.

Article Snippet: Quantitative analysis was performed with the ABI PRISM 7700 Sequence Detection System (AB Applied Biosystems, Life Technologies Corp.), to quantify the relative abundance of 16S rRNA genes of several groups: Genus Ruminococcus , genus Prevotella , Selenomonas ruminantium , Fibrobacter succinogenes , Megasphaera elsdenii , and Streptococcus spp. were all measured as a percentage of total bacterial 16S rRNA using previously reported primers .

Techniques:

Journal: Gut Microbes

Article Title: Cellulolytic bacteria in the large intestine of mammals

doi: 10.1080/19490976.2022.2031694

Figure Lengend Snippet: Cellulolytic strains isolated from the large intestine and type strains isolated from the rumen: collection number, authors and date of isolation, metabolism and accession number for rRNA 16S sequences. 20–24 , 26–28 , 44 , 52–54 , 62 , 68–73 The carbohydrates consumed and end products are indicated by the following letters: Glu, glucose; Cel, cellobiose; Mal, maltose; Lac, lactose; Ara, arabinose; Xyl, xylose; Gal, galactose; Fru, fructose; Sac, saccharose; Raf, raffinose; Rib, Ribose; Man, mannose; AG, galacturonic acid; Mel, melibiose; A, acetate; S, succinate; F, format; L, lactate; E, ethanol; H, hydrogen. ND, not determined

Article Snippet: Only two cellulolytic strains of the genus Ruminococcus isolated from the large intestine were deposited in collection: one strain of R. flavefaciens isolated from the pig cecum (strain C52; ATCC 49949) and, the type strain 18P13 of R. champanellensis isolated from the human feces (DSM 18848, JCM 17042).

Techniques: Isolation

Journal: Gut Microbes

Article Title: Cellulolytic bacteria in the large intestine of mammals

doi: 10.1080/19490976.2022.2031694

Figure Lengend Snippet: Molecular phylogenetic analysis of cellulolytic Ruminococcus found in the large intestine, the type strains R. albus 7 and R. flavefaciens C94, and the well-known strain FD1 using a maximum likelihood algorithm method (1,000 bootstrap trial). The numbers indicated for each branch represent bootstrap values. The phylogeny tree was constructed using a near-full-length 16S ribosomal RNA sequence (1315 bp) with MEGA 7.0 to the same length for all sequences.

Article Snippet: Only two cellulolytic strains of the genus Ruminococcus isolated from the large intestine were deposited in collection: one strain of R. flavefaciens isolated from the pig cecum (strain C52; ATCC 49949) and, the type strain 18P13 of R. champanellensis isolated from the human feces (DSM 18848, JCM 17042).

Techniques: Construct, Sequencing

Journal: Gut Microbes

Article Title: Cellulolytic bacteria in the large intestine of mammals

doi: 10.1080/19490976.2022.2031694

Figure Lengend Snippet: Denomination and numbers of cellulases encoded by the genome of cellulolytic bacteria stains isolated from the large intestine and of type strains isolated from the rumen. 16 , 74 , 96–101 .

Article Snippet: Only two cellulolytic strains of the genus Ruminococcus isolated from the large intestine were deposited in collection: one strain of R. flavefaciens isolated from the pig cecum (strain C52; ATCC 49949) and, the type strain 18P13 of R. champanellensis isolated from the human feces (DSM 18848, JCM 17042).

Techniques: Isolation