genomic tip 500 g kit  (Qiagen)


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    Name:
    QIAGEN Genomic-tip 500/G
    Description:
    For isolation of up to 500 µg high-molecular-weight DNA from a wide range of samples. Kit contents: Qiagen Genomic-tip 500/G, 10 Columns, Gravity-flow, Anion-exchange Tips, Reliable Isolation of DNA up to 150 kb in Size with an Average Size of 50 to 100 kb, Convenient, Parallel Processing of Multiple Samples, No Phenol or Chloroform Extractions, For Isolation of up to 20g High-molecular-weight DNA from a Wide Range of Samples. Benefits: Reliable isolation of DNA up to 150 kb in size. No phenol or chloroform extractions. Convenient, parallel processing of multiple samples
    Catalog Number:
    10262
    Price:
    None
    Category:
    QIAGEN Genomic-tip 500/G
    Score:
    85
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    Structured Review

    Qiagen genomic tip 500 g kit
    QIAGEN Genomic-tip 500/G
    For isolation of up to 500 µg high-molecular-weight DNA from a wide range of samples. Kit contents: Qiagen Genomic-tip 500/G, 10 Columns, Gravity-flow, Anion-exchange Tips, Reliable Isolation of DNA up to 150 kb in Size with an Average Size of 50 to 100 kb, Convenient, Parallel Processing of Multiple Samples, No Phenol or Chloroform Extractions, For Isolation of up to 20g High-molecular-weight DNA from a Wide Range of Samples. Benefits: Reliable isolation of DNA up to 150 kb in size. No phenol or chloroform extractions. Convenient, parallel processing of multiple samples
    https://www.bioz.com/result/genomic tip 500 g kit/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    genomic tip 500 g kit - by Bioz Stars, 2019-10
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: Complete Genome Assembly of Streptococcus pyogenes ATCC 19615, a Group A β-Hemolytic Reference Strain
    Article Snippet: Notably, this organism is the cause of up to 30% of childhood acute bacterial pharyngitis cases , and up to 20% of asymptomatic children may be carriers at various times of the year ( ). .. High-quality genomic DNA was extracted from a purified isolate using a Qiagen Genomic-tip 500 at the USAMRIID Diagnostic Systems Divisions (DSD). .. Specifically, a 100-mL bacterial culture was grown to stationary phase and nucleic acid extracted as per manufacturer’s recommendations.

    Clone Assay:

    Article Title: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice
    Article Snippet: Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). .. The partial digests were electrophoretically separated on a 0.8% agarose gel and the 300 to 500 bp range of DNA fragments extracted and ligated in a 1∶1 molar ratio with EcoRI-digested and Antarctic phosphatase-treated pBbIVET.

    Article Title: Big Genomes Facilitate the Comparative Identification of Regulatory Elements
    Article Snippet: High molecular weight genomic DNA was obtained from approximately 500 mg of frozen adult flies using the Qiagen 500/G Genomic-tip protocol for isolation of genomic DNA from flies (Qiagen Cat. No. 10262). .. Fosmid libraries were generated according to the Fosmid (40 kb) Library Creation Protocol developed at the DOE Joint Genome Institute ( http://www.jgi.doe.gov/sequencing/protocols/prots_production.html ) with the following modifications.

    Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8
    Article Snippet: Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany). .. The resulting DNA fragments were separated by gel electrophoresis.

    Centrifugation:

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: SDS was precipitated with 3 mL buffer L5 (3 M potassium acetate [pH 7.5]), and the cloudy solution was clarified by centrifugation at 15,000 RCF for 30 minutes at 4 °C. .. The soluble phage DNA was passed over a pre-equilibrated Qiagen tip-500 column (Qiagen #10262), washed with buffer QC (1.0 M NaCl, 50 mM MOPS [pH 7.0], 15% isopropanol) and eluted with 15 mL buffer QF (1.25 M NaCl, 50 mM Tris [pH 8.5], 15% isopropanol).

    Scaffolding:

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform. .. DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform.

    Incubation:

    Article Title: Big Genomes Facilitate the Comparative Identification of Regulatory Elements
    Article Snippet: High molecular weight genomic DNA was obtained from approximately 500 mg of frozen adult flies using the Qiagen 500/G Genomic-tip protocol for isolation of genomic DNA from flies (Qiagen Cat. No. 10262). .. DNA was end-repaired without hydro shearing, phenol-extracted, and precipitated a second time after gel-purification to increase cloning efficiency.

    Article Title: Protective Antigens Against Glanders Identified by Expression Library Immunization
    Article Snippet: Buffer 2 (0.35 ml) was added and tubes were mixed and incubated at 50°C for 30 min in a heatblock. .. Lysates were added to a Qiagen Genomic-tip 500/G purification column and genomic DNA was extracted following manufacturer’s instructions.

    Article Title: Genome Sequence of the Deep-Sea Bacterium Idiomarina abyssalis KMM 227T
    Article Snippet: I. abyssalis was rehydrated and cultured in marine broth and incubated at 30°C for 72 h at atmospheric pressure. .. Extraction of gDNA was performed using the Genomic-tip 500/g kit (Qiagen, Valencia, CA).

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: The phage capsid proteins were further digested by incubation with 100 ng μL−1 of proteinase K (NEB #P8012S) for 1 hour at 55 °C. .. The soluble phage DNA was passed over a pre-equilibrated Qiagen tip-500 column (Qiagen #10262), washed with buffer QC (1.0 M NaCl, 50 mM MOPS [pH 7.0], 15% isopropanol) and eluted with 15 mL buffer QF (1.25 M NaCl, 50 mM Tris [pH 8.5], 15% isopropanol).

    Article Title: Genome analysis of Diploscapter coronatus: insights into molecular peculiarities of a nematode with parthenogenetic reproduction
    Article Snippet: The nematodes were transferred to a 1-l flask containing 100 ml of distilled water and incubated for 2 h to allow digestion of remaining food bacteria. .. Genomic DNA was purified with the Genomic-tip 500/G Kit, according to the manufacturer’s instructions (Qiagen, Hilden, Germany).

    Article Title: Comparative genomic analyses reveal the features for adaptation to nematodes in fungi
    Article Snippet: Genomic DNA was extracted using Qiagen Genomic-tip Kit 500 G (Cat No./ID: 10262, Germany) according to the manufacturer’s instructions. .. Genomic DNA was extracted using Qiagen Genomic-tip Kit 500 G (Cat No./ID: 10262, Germany) according to the manufacturer’s instructions.

    Modification:

    Article Title: Complete genome sequence of Desulfurococcus mucosus type strain (O7/1T)
    Article Snippet: D. mucosus strain 07/1T , DSM 2162, was grown anaerobically in DSMZ medium 184 (Desulfurococcus medium) [ ] at 85°C. .. DNA was isolated from 0.5-1 g of cell paste using Qiagen Genomic 500 DNA kit (Qiagen 10262) following the standard protocol as recommended by the manufacturer, with no modification. .. DNA is available through the DNA Bank Network [ ].

    Article Title: Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun
    Article Snippet: The total genomic DNA from the tissue of each fruiting body was extracted using the CTAB method slightly modified for higher fungi . .. However, due to the old age and deterioration of the type specimen, a different method was used to extract the genomic DNA, using the QIAGEN Genomic-tip 500/G (QIAGEN, Cat. 10262, Germany) kit, following the instructions provided by the manufacturer.

    Transformation Assay:

    Article Title: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice
    Article Snippet: Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). .. Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen).

    Gel Purification:

    Article Title: Big Genomes Facilitate the Comparative Identification of Regulatory Elements
    Article Snippet: High molecular weight genomic DNA was obtained from approximately 500 mg of frozen adult flies using the Qiagen 500/G Genomic-tip protocol for isolation of genomic DNA from flies (Qiagen Cat. No. 10262). .. Fosmid libraries were generated according to the Fosmid (40 kb) Library Creation Protocol developed at the DOE Joint Genome Institute ( http://www.jgi.doe.gov/sequencing/protocols/prots_production.html ) with the following modifications.

    Electroporation:

    Article Title: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice
    Article Snippet: Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). .. Library ligations were electroporated into E. coli Top10 cells (Life Technologies) and transformants selected on LB agar containing 50 µg/ml kanamycin, resulting in approximately 30,000 independent clones.

    Gas Chromatography:

    Article Title: Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken
    Article Snippet: Long fragments of genomic DNA (gDNA) were prepared by using the procedure of Neumann et al. ( ) and further purified using the Genomic-tip 500/G (Qiagen). .. Single-molecule real-time (SMRT) sequencing and de novo genome assembly were done at the University of Delaware Sequencing and Genotyping Center using the PacBio RS II SMRT DNA sequencing system (Pacific Biosciences, Menlo Park, CA) and HGAP Assembly.3 software, respectively.

    Ligation:

    Article Title: Big Genomes Facilitate the Comparative Identification of Regulatory Elements
    Article Snippet: High molecular weight genomic DNA was obtained from approximately 500 mg of frozen adult flies using the Qiagen 500/G Genomic-tip protocol for isolation of genomic DNA from flies (Qiagen Cat. No. 10262). .. DNA was end-repaired without hydro shearing, phenol-extracted, and precipitated a second time after gel-purification to increase cloning efficiency.

    Article Title: Complete Genome Sequences of Six Listeria monocytogenes Sequence Type 9 Isolates from Meat Processing Plants in Norway
    Article Snippet: For the purification of genomic DNA, cells were lysed using lysing matrix B and a FastPrep instrument (both MP Biomedicals), and DNA was isolated using the DNeasy blood and tissue kit (Qiagen), except for the MF4545 sample sequenced on the MinION instrument, for which cells were lysed using mutanolysin and DNA isolated using the Qiagen Genomic-tip 500/G kit. .. For the purification of genomic DNA, cells were lysed using lysing matrix B and a FastPrep instrument (both MP Biomedicals), and DNA was isolated using the DNeasy blood and tissue kit (Qiagen), except for the MF4545 sample sequenced on the MinION instrument, for which cells were lysed using mutanolysin and DNA isolated using the Qiagen Genomic-tip 500/G kit.

    Cell Culture:

    Article Title: Comparative Genomic Characterization of Three Streptococcus parauberis Strains in Fish Pathogen, as Assessed by Wide-Genome Analyses
    Article Snippet: The control, S. parauberis KCTC 11537 (GenBank accession no. NC_015558), was as previously described [ ]. .. The two S. parauberis strains were cultured in THB at 25°C for 24 h, and genomic DNA was extracted using the Qiagen Genomic-tip 500/G kit (Qiagen, Hilden, Germany) and a genomic DNA buffer set (Qiagen), according to the manufacturer’s instructions. .. The draft genomes of S. parauberis KRS-02083 and KRS-02109 were sequenced by the National Research Institute of Fisheries Science (Yokohama, Japan) using a Roche/454 GS-FLXTM system [ ], and Takara Bio, Inc. (Otsu, Japan) using Illumina Genome Analyzer (GA) [ ].

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: The two genetic variant strains of E. piscicida , JF1305 and RSB1309, were cultured in 10 ml brain heart infusion broth overnight at 27°C. .. The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set.

    Article Title: Protective Antigens Against Glanders Identified by Expression Library Immunization
    Article Snippet: To isolate genomic DNA, bacilli were cultured at 37°C in LB broth for 18 h and then pelleted at 3000–5000 g for 5–10 min at room temperature. .. Lysates were added to a Qiagen Genomic-tip 500/G purification column and genomic DNA was extracted following manufacturer’s instructions.

    Article Title: Genome Sequence of the Deep-Sea Bacterium Idiomarina abyssalis KMM 227T
    Article Snippet: After rehydration, the bacterium was cultured in log-phase growth before its genomic DNA (gDNA) was obtained. .. Extraction of gDNA was performed using the Genomic-tip 500/g kit (Qiagen, Valencia, CA).

    Generated:

    Article Title: Complete Genome Assembly of Streptococcus pyogenes ATCC 19615, a Group A β-Hemolytic Reference Strain
    Article Snippet: High-quality genomic DNA was extracted from a purified isolate using a Qiagen Genomic-tip 500 at the USAMRIID Diagnostic Systems Divisions (DSD). .. High-quality genomic DNA was extracted from a purified isolate using a Qiagen Genomic-tip 500 at the USAMRIID Diagnostic Systems Divisions (DSD).

    Transmission Assay:

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: This strain and its genome sequence will be useful for performing functional studies of gametocytogenesis, mosquito transmission, and mosquito stage development. .. DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform.

    Polymerase Chain Reaction:

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: Based on the PCR results, the isolates from olive flounder and red sea bream were classified as E. piscicida and E. piscicida -like species, respectively. .. The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set.

    Article Title: Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella enteritidis within phage type
    Article Snippet: For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria. .. For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria.

    Sonication:

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform. .. Library preparation and sequencing were performed by GATC Biotech (Constance, Germany) on a PacBio RS II sequencer, with an average read length of 16,291 bp and genome coverage of 100× postfiltering.

    Recombinant:

    Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8
    Article Snippet: Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany). .. Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany).

    Molecular Weight:

    Article Title: Big Genomes Facilitate the Comparative Identification of Regulatory Elements
    Article Snippet: The propidium iodide fluorescence and genome size of Gallus domesticus (red blood cell standard, 1,225 Mb) and D. melanogaster (174 Mb) were used to calculate the unknown genome sizes. .. High molecular weight genomic DNA was obtained from approximately 500 mg of frozen adult flies using the Qiagen 500/G Genomic-tip protocol for isolation of genomic DNA from flies (Qiagen Cat. No. 10262). .. Fosmid libraries were generated according to the Fosmid (40 kb) Library Creation Protocol developed at the DOE Joint Genome Institute ( http://www.jgi.doe.gov/sequencing/protocols/prots_production.html ) with the following modifications.

    DNA Extraction:

    Article Title: Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella enteritidis within phage type
    Article Snippet: Paragraph title: DNA isolation ... For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria.

    Article Title: Protective Antigens Against Glanders Identified by Expression Library Immunization
    Article Snippet: Paragraph title: B. mallei culturing and genomic DNA isolation ... Lysates were added to a Qiagen Genomic-tip 500/G purification column and genomic DNA was extracted following manufacturer’s instructions.

    Article Title: Complete genome sequence of Desulfurococcus mucosus type strain (O7/1T)
    Article Snippet: Paragraph title: Growth conditions and DNA isolation ... DNA was isolated from 0.5-1 g of cell paste using Qiagen Genomic 500 DNA kit (Qiagen 10262) following the standard protocol as recommended by the manufacturer, with no modification.

    Nucleic Acid Electrophoresis:

    Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8
    Article Snippet: Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany). .. To construct plasmid libraries for Sanger sequencing, the DNA was sheared by employing a Hydroshear as described by the manufacturer (GeneMachines, San Carlos, CA, USA).

    RNA Sequencing Assay:

    Article Title: Clades of Photosynthetic Bacteria Belonging to the Genus Rhodopseudomonas Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
    Article Snippet: Paragraph title: Genome sequencing and RNA-seq analysis. ... Genomic DNA was extracted using the Qiagen Genomic-tip 500/G kit and sequenced using paired-end sequencing with Illumina HiSeq 2000 ( ).

    Isolation:

    Article Title: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice
    Article Snippet: The clones that retained the plasmid content of the parent clone were used in further experiments. .. Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). .. A3 genomic DNA was partially digested with Tsp509I (New England Biolabs).

    Article Title: Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of Corynebacterium Cluster 3 Species
    Article Snippet: On the other hand, C. falsenii was isolated from the respiratory tracts of eagles and black storks ( , ) and from bioaerosols sampled in duck houses ( ). .. Genomic DNA was purified with the Genomic-tip 500/G system and the Genomic DNA buffer set (Qiagen), and it was used as starting material to prepare a standard sequencing library according to the workflow of the Nextera DNA sample preparation kit (Illumina).

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: We isolated Edwardsiella in May 2013 from olive flounder cultured in Kagoshima Prefecture, Japan, and in September 2013 from red sea bream cultured in Kochi Prefecture, Japan. .. The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set.

    Article Title: Big Genomes Facilitate the Comparative Identification of Regulatory Elements
    Article Snippet: The propidium iodide fluorescence and genome size of Gallus domesticus (red blood cell standard, 1,225 Mb) and D. melanogaster (174 Mb) were used to calculate the unknown genome sizes. .. High molecular weight genomic DNA was obtained from approximately 500 mg of frozen adult flies using the Qiagen 500/G Genomic-tip protocol for isolation of genomic DNA from flies (Qiagen Cat. No. 10262). .. Fosmid libraries were generated according to the Fosmid (40 kb) Library Creation Protocol developed at the DOE Joint Genome Institute ( http://www.jgi.doe.gov/sequencing/protocols/prots_production.html ) with the following modifications.

    Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8
    Article Snippet: Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany). .. The resulting DNA fragments were separated by gel electrophoresis.

    Article Title: Complete genome sequence of Desulfurococcus mucosus type strain (O7/1T)
    Article Snippet: D. mucosus strain 07/1T , DSM 2162, was grown anaerobically in DSMZ medium 184 (Desulfurococcus medium) [ ] at 85°C. .. DNA was isolated from 0.5-1 g of cell paste using Qiagen Genomic 500 DNA kit (Qiagen 10262) following the standard protocol as recommended by the manufacturer, with no modification. .. DNA is available through the DNA Bank Network [ ].

    Article Title: Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken
    Article Snippet: B. thermosphacta was the bacterial species most frequently isolated from ground chicken samples in our previous study on the nonspecific binding of food-associated microbial flora to immunomagnetic microspheres ( ). .. Long fragments of genomic DNA (gDNA) were prepared by using the procedure of Neumann et al. ( ) and further purified using the Genomic-tip 500/G (Qiagen).

    Article Title: Complete Genome Sequences of Six Listeria monocytogenes Sequence Type 9 Isolates from Meat Processing Plants in Norway
    Article Snippet: MF4624, MF4626, and MF4697 were from plant M4 with MLVA profile 22. .. For the purification of genomic DNA, cells were lysed using lysing matrix B and a FastPrep instrument (both MP Biomedicals), and DNA was isolated using the DNeasy blood and tissue kit (Qiagen), except for the MF4545 sample sequenced on the MinION instrument, for which cells were lysed using mutanolysin and DNA isolated using the Qiagen Genomic-tip 500/G kit. .. For Illumina sequencing, Nextera XT libraries were prepared and sequenced either on a MiSeq platform with 300-bp paired-end (PE) reads (strains MF4545, MF4562, MF4624, and MF4697) or on a HiSeq platform with 150-bp PE reads (strains MF4626 and MF6172).

    Article Title: Comparative genomic analyses reveal the features for adaptation to nematodes in fungi
    Article Snippet: This fungus was originally isolated from Scolytus intricatus and its galleries in oak trees in Czech Republic. .. Genomic DNA was extracted using Qiagen Genomic-tip Kit 500 G (Cat No./ID: 10262, Germany) according to the manufacturer’s instructions.

    Purification:

    Article Title: Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of Corynebacterium Cluster 3 Species
    Article Snippet: C. falsenii DSM 44353 (BL 8171, CCUG 33651) was obtained from the Leibniz Institute DSMZ (Braunschweig) and grown in brain heart infusion broth-yeast extract at 37°C ( ). .. Genomic DNA was purified with the Genomic-tip 500/G system and the Genomic DNA buffer set (Qiagen), and it was used as starting material to prepare a standard sequencing library according to the workflow of the Nextera DNA sample preparation kit (Illumina). .. The genomic library was sequenced in a 2 × 250 nucleotide (nt) paired-end run using the MiSeq reagent kit version 2 and the MiSeq desktop sequencer (Illumina), resulting in 1,077,270 reads and an 81-fold genome coverage.

    Article Title: Protective Antigens Against Glanders Identified by Expression Library Immunization
    Article Snippet: Buffer 2 (0.35 ml) was added and tubes were mixed and incubated at 50°C for 30 min in a heatblock. .. Lysates were added to a Qiagen Genomic-tip 500/G purification column and genomic DNA was extracted following manufacturer’s instructions. .. The genomic sequence of the two chromosomes of B. mallei ATCC23344 were downloaded from GenBank (accession no. CP000010; CP000011).

    Article Title: Complete Genome Assembly of Streptococcus pyogenes ATCC 19615, a Group A β-Hemolytic Reference Strain
    Article Snippet: Notably, this organism is the cause of up to 30% of childhood acute bacterial pharyngitis cases , and up to 20% of asymptomatic children may be carriers at various times of the year ( ). .. High-quality genomic DNA was extracted from a purified isolate using a Qiagen Genomic-tip 500 at the USAMRIID Diagnostic Systems Divisions (DSD). .. Specifically, a 100-mL bacterial culture was grown to stationary phase and nucleic acid extracted as per manufacturer’s recommendations.

    Article Title: Efficient modification of λ-DNA substrates for single-molecule studies
    Article Snippet: The soluble phage DNA was passed over a pre-equilibrated Qiagen tip-500 column (Qiagen #10262), washed with buffer QC (1.0 M NaCl, 50 mM MOPS [pH 7.0], 15% isopropanol) and eluted with 15 mL buffer QF (1.25 M NaCl, 50 mM Tris [pH 8.5], 15% isopropanol). .. Finally, the DNA was precipitated with the addition of 10.5 mL of 100% isopropanol, rinsed in 70% ethanol and re-dissolved in TE buffer (10 mM Tris-HCl [pH 8.0], 1 mM EDTA) to a final DNA concentration of 200–500 ng μL−1 .

    Article Title: Genome analysis of Diploscapter coronatus: insights into molecular peculiarities of a nematode with parthenogenetic reproduction
    Article Snippet: 200–400 mg of packed worms were ground in a mortar in liquid nitrogen and used for a single DNA/RNA preparation. .. Genomic DNA was purified with the Genomic-tip 500/G Kit, according to the manufacturer’s instructions (Qiagen, Hilden, Germany). .. RNA was purified by RNAgents Total RNA Isolation System (Promega, Fitchburg, WI, USA) and polyadenylated RNA was purified with a mRNA Purification Kit (GE Healthcare Life Sciences, Buckinghamshire, UK) using an Oligo(dT)-cellulose column.

    Article Title: Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken
    Article Snippet: In conjunction with studies of the unusual growth phenotype exhibited by strain BII, the complete genome sequences of both strains BI and BII were determined. .. Long fragments of genomic DNA (gDNA) were prepared by using the procedure of Neumann et al. ( ) and further purified using the Genomic-tip 500/G (Qiagen). .. Single-molecule real-time (SMRT) sequencing and de novo genome assembly were done at the University of Delaware Sequencing and Genotyping Center using the PacBio RS II SMRT DNA sequencing system (Pacific Biosciences, Menlo Park, CA) and HGAP Assembly.3 software, respectively.

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: Late-stage parasites were grown in 6 ml of concentrated blood at 3.5% parasitemia and enriched by Plasmagel flotation. .. DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform. .. Library preparation and sequencing were performed by GATC Biotech (Constance, Germany) on a PacBio RS II sequencer, with an average read length of 16,291 bp and genome coverage of 100× postfiltering.

    Sequencing:

    Article Title: Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of Corynebacterium Cluster 3 Species
    Article Snippet: C. falsenii DSM 44353 (BL 8171, CCUG 33651) was obtained from the Leibniz Institute DSMZ (Braunschweig) and grown in brain heart infusion broth-yeast extract at 37°C ( ). .. Genomic DNA was purified with the Genomic-tip 500/G system and the Genomic DNA buffer set (Qiagen), and it was used as starting material to prepare a standard sequencing library according to the workflow of the Nextera DNA sample preparation kit (Illumina). .. The genomic library was sequenced in a 2 × 250 nucleotide (nt) paired-end run using the MiSeq reagent kit version 2 and the MiSeq desktop sequencer (Illumina), resulting in 1,077,270 reads and an 81-fold genome coverage.

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set. .. Genome sequencing was performed on a 454 GS Junior System (Roche), which generated 132,085 reads for strain JF1305 and 162,723 reads for strain RSB1309.

    Article Title: Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella enteritidis within phage type
    Article Snippet: For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria. .. For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria.

    Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8
    Article Snippet: Paragraph title: Genome sequencing, assembly and gap closure ... Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany).

    Article Title: Complete Genome Assembly of Streptococcus pyogenes ATCC 19615, a Group A β-Hemolytic Reference Strain
    Article Snippet: High-quality genomic DNA was extracted from a purified isolate using a Qiagen Genomic-tip 500 at the USAMRIID Diagnostic Systems Divisions (DSD). .. High-quality genomic DNA was extracted from a purified isolate using a Qiagen Genomic-tip 500 at the USAMRIID Diagnostic Systems Divisions (DSD).

    Article Title: Genome Sequence of the Deep-Sea Bacterium Idiomarina abyssalis KMM 227T
    Article Snippet: Extraction of gDNA was performed using the Genomic-tip 500/g kit (Qiagen, Valencia, CA). .. Extraction of gDNA was performed using the Genomic-tip 500/g kit (Qiagen, Valencia, CA).

    Article Title: Clades of Photosynthetic Bacteria Belonging to the Genus Rhodopseudomonas Show Marked Diversity in Light-Harvesting Antenna Complex Gene Composition and Expression
    Article Snippet: E. coli cultures were supplemented with Gm at 20 µg/ml. .. Genomic DNA was extracted using the Qiagen Genomic-tip 500/G kit and sequenced using paired-end sequencing with Illumina HiSeq 2000 ( ). .. Sequence reads were assembled using Velvet version 1.1.07 ( ).

    Article Title: Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun
    Article Snippet: The procedures for ITS sequencing followed those described previously , using the ITS4 and ITS5 primers . .. However, due to the old age and deterioration of the type specimen, a different method was used to extract the genomic DNA, using the QIAGEN Genomic-tip 500/G (QIAGEN, Cat. 10262, Germany) kit, following the instructions provided by the manufacturer.

    Article Title: Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken
    Article Snippet: Long fragments of genomic DNA (gDNA) were prepared by using the procedure of Neumann et al. ( ) and further purified using the Genomic-tip 500/G (Qiagen). .. Assembly of the B. thermospacta BI and BII genomes resulted in single contigs of 2,647,794 bp (36.4% GC content) and 2,603,084 bp (36.5% GC content), respectively.

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: This strain and its genome sequence will be useful for performing functional studies of gametocytogenesis, mosquito transmission, and mosquito stage development. .. DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform.

    Gradient Centrifugation:

    Article Title: A Novel CpG Island Set Identifies Tissue-Specific Methylation at Developmental Gene Loci
    Article Snippet: Monocyte and granulocyte cells were prepared from whole human blood using Ficoll gradient centrifugation. .. Whole human blood, monocyte, and granulocyte DNA was extracted using the Genomic-tip 500/G (Qiagen 10262) genomic DNA purification kit.

    Agarose Gel Electrophoresis:

    Article Title: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice
    Article Snippet: Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). .. Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen).

    Plasmid Preparation:

    Article Title: In Vivo Expression Technology Identifies a Novel Virulence Factor Critical for Borrelia burgdorferi Persistence in Mice
    Article Snippet: Total genomic DNA was isolated from a 250 ml culture of B. burgdorferi B31 clone A3 grown to a density 1×108 spirochetes/ml using the Qiagen genomic DNA buffer set and Genomic-tip 500/G, according to the manufacturer's protocol (Qiagen). .. Library ligations were electroporated into E. coli Top10 cells (Life Technologies) and transformants selected on LB agar containing 50 µg/ml kanamycin, resulting in approximately 30,000 independent clones.

    Article Title: Sequencing, annotation, and comparative genome analysis of the gerbil-adapted Helicobacter pylori strain B8
    Article Snippet: Total genomic DNA of a liquid H. pylori B8 culture (Brucella broth, 10% FCS, streptomycin 250 mg/l) was extracted by using a genomic-tip G-500 (Qiagen, Hilden, Germany). .. The resulting DNA fragments were separated by gel electrophoresis.

    Software:

    Article Title: Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of Corynebacterium Cluster 3 Species
    Article Snippet: Genomic DNA was purified with the Genomic-tip 500/G system and the Genomic DNA buffer set (Qiagen), and it was used as starting material to prepare a standard sequencing library according to the workflow of the Nextera DNA sample preparation kit (Illumina). .. Genomic DNA was purified with the Genomic-tip 500/G system and the Genomic DNA buffer set (Qiagen), and it was used as starting material to prepare a standard sequencing library according to the workflow of the Nextera DNA sample preparation kit (Illumina).

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set. .. Genome sequencing was performed on a 454 GS Junior System (Roche), which generated 132,085 reads for strain JF1305 and 162,723 reads for strain RSB1309.

    Functional Assay:

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: This strain and its genome sequence will be useful for performing functional studies of gametocytogenesis, mosquito transmission, and mosquito stage development. .. DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform.

    Binding Assay:

    Article Title: Complete Genome Sequences of Two Strains of the Meat Spoilage Bacterium Brochothrix thermosphacta Isolated from Ground Chicken
    Article Snippet: B. thermosphacta was the bacterial species most frequently isolated from ground chicken samples in our previous study on the nonspecific binding of food-associated microbial flora to immunomagnetic microspheres ( ). .. Long fragments of genomic DNA (gDNA) were prepared by using the procedure of Neumann et al. ( ) and further purified using the Genomic-tip 500/G (Qiagen).

    Sample Prep:

    Article Title: Complete Genome Sequence of Corynebacterium falsenii DSM 44353 To Study the Evolution of Corynebacterium Cluster 3 Species
    Article Snippet: C. falsenii DSM 44353 (BL 8171, CCUG 33651) was obtained from the Leibniz Institute DSMZ (Braunschweig) and grown in brain heart infusion broth-yeast extract at 37°C ( ). .. Genomic DNA was purified with the Genomic-tip 500/G system and the Genomic DNA buffer set (Qiagen), and it was used as starting material to prepare a standard sequencing library according to the workflow of the Nextera DNA sample preparation kit (Illumina). .. The genomic library was sequenced in a 2 × 250 nucleotide (nt) paired-end run using the MiSeq reagent kit version 2 and the MiSeq desktop sequencer (Illumina), resulting in 1,077,270 reads and an 81-fold genome coverage.

    In Vitro:

    Article Title: De Novo Genome Assembly of a Plasmodium falciparum NF54 Clone Using Single-Molecule Real-Time Sequencing
    Article Snippet: However, 3D7 produces fewer gametocytes under in vitro culture conditions than the original NF54 strain ( ). .. DNA was extracted with the Genomic-tip 500/G kit (catalog number 10262; Qiagen) and further purified with phenol-chloroform.

    Concentration Assay:

    Article Title: Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella enteritidis within phage type
    Article Snippet: For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria. .. For mutational mapping and associated re-sequencing services (Nimblegen, Inc.), total DNA was extracted using a Qiagen Genomic-tip 500/G kit following the protocol designated for bacteria.

    DNA Purification:

    Article Title: A Novel CpG Island Set Identifies Tissue-Specific Methylation at Developmental Gene Loci
    Article Snippet: Mononucleocytes were recovered from the plasma-ficoll interphase and granulocytes from the cell pellet. .. Whole human blood, monocyte, and granulocyte DNA was extracted using the Genomic-tip 500/G (Qiagen 10262) genomic DNA purification kit. .. Sperm DNA was prepared as described [ ].

    Lysis:

    Article Title: Protective Antigens Against Glanders Identified by Expression Library Immunization
    Article Snippet: The cells were resuspended in 1 ml of buffer B1 (with RNase A; Qiagen, Valencia, CA, USA) to obtain efficient lysis of the bacteria, then 20 μl of lysozyme stock solution and 45 μl of Proteinase K solution were added and the samples were incubated at 37°C for at least 30 min. .. Lysates were added to a Qiagen Genomic-tip 500/G purification column and genomic DNA was extracted following manufacturer’s instructions.

    Variant Assay:

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: The two genetic variant strains of E. piscicida , JF1305 and RSB1309, were cultured in 10 ml brain heart infusion broth overnight at 27°C. .. The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set.

    Fluorescence In Situ Hybridization:

    Article Title: Draft Genome Sequences of Two Genetic Variant Strains of Edwardsiella piscicida, JF1305 and RSB1309, Isolated from Olive Flounder (Paralichythys olivaceus) and Red Sea Bream (Pagrus major) Cultured in Japan, Respectively
    Article Snippet: The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set. .. The genomic DNA was extracted with a Qiagen Genomic-tip 500/G kit and a genomic DNA buffer set.

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    Qiagen genomic tip kit
    Genomic Tip Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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