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Retrogen genomic pcr
Genomic Pcr, supplied by Retrogen, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic pcr/product/Retrogen
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
genomic pcr - by Bioz Stars, 2020-05
93/100 stars

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Polymerase Chain Reaction:

Article Title: Function of Golgi-centrosome proximity in RPE-1 cells
Article Snippet: .. Approximately 130 clones were screened by immunofluorescence, and two clones were selected for further verification by western blotting and genomic PCR followed by sequencing (Retrogen). .. Immunofluorescence microscopy Cells were grown on glass coverslips and fixed with ice-cold methanol (JT Baker) for 7 minutes or with 4% paraformaldehyde (TedPella) for 10 minutes.

Clone Assay:

Article Title: Function of Golgi-centrosome proximity in RPE-1 cells
Article Snippet: .. Approximately 130 clones were screened by immunofluorescence, and two clones were selected for further verification by western blotting and genomic PCR followed by sequencing (Retrogen). .. Immunofluorescence microscopy Cells were grown on glass coverslips and fixed with ice-cold methanol (JT Baker) for 7 minutes or with 4% paraformaldehyde (TedPella) for 10 minutes.

Sequencing:

Article Title: Function of Golgi-centrosome proximity in RPE-1 cells
Article Snippet: .. Approximately 130 clones were screened by immunofluorescence, and two clones were selected for further verification by western blotting and genomic PCR followed by sequencing (Retrogen). .. Immunofluorescence microscopy Cells were grown on glass coverslips and fixed with ice-cold methanol (JT Baker) for 7 minutes or with 4% paraformaldehyde (TedPella) for 10 minutes.

Immunofluorescence:

Article Title: Function of Golgi-centrosome proximity in RPE-1 cells
Article Snippet: .. Approximately 130 clones were screened by immunofluorescence, and two clones were selected for further verification by western blotting and genomic PCR followed by sequencing (Retrogen). .. Immunofluorescence microscopy Cells were grown on glass coverslips and fixed with ice-cold methanol (JT Baker) for 7 minutes or with 4% paraformaldehyde (TedPella) for 10 minutes.

Western Blot:

Article Title: Function of Golgi-centrosome proximity in RPE-1 cells
Article Snippet: .. Approximately 130 clones were screened by immunofluorescence, and two clones were selected for further verification by western blotting and genomic PCR followed by sequencing (Retrogen). .. Immunofluorescence microscopy Cells were grown on glass coverslips and fixed with ice-cold methanol (JT Baker) for 7 minutes or with 4% paraformaldehyde (TedPella) for 10 minutes.

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  • 92
    Retrogen pgl3 basic
    Mapping the core promoter region of human Sell gene. ( A ) 5′ serial deletion mutants shown on the left side were transiently transfected into Jurkat (stripped bars), EL4 (open bars), or HeLa cells (solid bars) and Luciferase activity shown on the right side was analyzed 30 hours after transfection. ( B ) Jurkat cells were co-transfected with core promoter construct, hSell288, with plasmids expressing Sp1, Mzf1, Klf2, Irf1, Ets1. Notes: Luciferase activity was analyzed 30 hours after the co-transfection. Luciferase activity was expressed as percentage of that of <t>pGL3-Promoter</t> in 2A and as fold changes relative to that of pGL3 vector transfected Jurkat cells in 2B. Data shown are mean ± SD of three independent transfections in one experiment. Each experiment was repeated at least three times.
    Pgl3 Basic, supplied by Retrogen, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl3 basic/product/Retrogen
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pgl3 basic - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Retrogen human rdhe2s
    Expression and characterization of human <t>RDHE2S</t> variants (A) Western blot analysis of human RDHE2Ss in HEK 293 cell lysates (25 μg). The corresponding protein was detected using FLAG antibodies at a 1:3,000 dilution and is indicated by an arrow; *non-specific band serves as a loading control. (B) Northern blot analysis of mRNA levels produced by expression vectors for the short (RDHE2Ss) and long (RDHE2Sl) forms of human RDHE2S; mock, HEK293 cells transfected with empty vector. (C) Western blot analysis of subcellular fractions isolated from Sf9 cells expressing FLAG-tagged RDHE2Ss and RDHE2Sl. P, pellet; S, supernatant. For 3,000 g P, 10,000 g P, and 105,000 g S, 40 μg of protein was loaded. For 105,000 g P, 10 μg of protein was loaded. The data are representative of at least 3 independent experiments.
    Human Rdhe2s, supplied by Retrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human rdhe2s/product/Retrogen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human rdhe2s - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    Image Search Results


    Mapping the core promoter region of human Sell gene. ( A ) 5′ serial deletion mutants shown on the left side were transiently transfected into Jurkat (stripped bars), EL4 (open bars), or HeLa cells (solid bars) and Luciferase activity shown on the right side was analyzed 30 hours after transfection. ( B ) Jurkat cells were co-transfected with core promoter construct, hSell288, with plasmids expressing Sp1, Mzf1, Klf2, Irf1, Ets1. Notes: Luciferase activity was analyzed 30 hours after the co-transfection. Luciferase activity was expressed as percentage of that of pGL3-Promoter in 2A and as fold changes relative to that of pGL3 vector transfected Jurkat cells in 2B. Data shown are mean ± SD of three independent transfections in one experiment. Each experiment was repeated at least three times.

    Journal: Gene Regulation and Systems Biology

    Article Title: FOXO1 Up-Regulates Human L-selectin Expression Through Binding to a Consensus FOXO1 Motif

    doi: 10.4137/GRSB.S10343

    Figure Lengend Snippet: Mapping the core promoter region of human Sell gene. ( A ) 5′ serial deletion mutants shown on the left side were transiently transfected into Jurkat (stripped bars), EL4 (open bars), or HeLa cells (solid bars) and Luciferase activity shown on the right side was analyzed 30 hours after transfection. ( B ) Jurkat cells were co-transfected with core promoter construct, hSell288, with plasmids expressing Sp1, Mzf1, Klf2, Irf1, Ets1. Notes: Luciferase activity was analyzed 30 hours after the co-transfection. Luciferase activity was expressed as percentage of that of pGL3-Promoter in 2A and as fold changes relative to that of pGL3 vector transfected Jurkat cells in 2B. Data shown are mean ± SD of three independent transfections in one experiment. Each experiment was repeated at least three times.

    Article Snippet: The PCR products were gel-purified and cloned into pGL3-Basic, and sequence identity was confirmed by DNA sequencing (Retrogen).

    Techniques: Transfection, Luciferase, Activity Assay, Construct, Expressing, Cotransfection, Plasmid Preparation

    Expression and characterization of human RDHE2S variants (A) Western blot analysis of human RDHE2Ss in HEK 293 cell lysates (25 μg). The corresponding protein was detected using FLAG antibodies at a 1:3,000 dilution and is indicated by an arrow; *non-specific band serves as a loading control. (B) Northern blot analysis of mRNA levels produced by expression vectors for the short (RDHE2Ss) and long (RDHE2Sl) forms of human RDHE2S; mock, HEK293 cells transfected with empty vector. (C) Western blot analysis of subcellular fractions isolated from Sf9 cells expressing FLAG-tagged RDHE2Ss and RDHE2Sl. P, pellet; S, supernatant. For 3,000 g P, 10,000 g P, and 105,000 g S, 40 μg of protein was loaded. For 105,000 g P, 10 μg of protein was loaded. The data are representative of at least 3 independent experiments.

    Journal: Chemico-biological interactions

    Article Title: Characterization of human Short Chain Dehydrogenase/Reductase SDR16C family members related to Retinol Dehydrogenase 10

    doi: 10.1016/j.cbi.2016.10.019

    Figure Lengend Snippet: Expression and characterization of human RDHE2S variants (A) Western blot analysis of human RDHE2Ss in HEK 293 cell lysates (25 μg). The corresponding protein was detected using FLAG antibodies at a 1:3,000 dilution and is indicated by an arrow; *non-specific band serves as a loading control. (B) Northern blot analysis of mRNA levels produced by expression vectors for the short (RDHE2Ss) and long (RDHE2Sl) forms of human RDHE2S; mock, HEK293 cells transfected with empty vector. (C) Western blot analysis of subcellular fractions isolated from Sf9 cells expressing FLAG-tagged RDHE2Ss and RDHE2Sl. P, pellet; S, supernatant. For 3,000 g P, 10,000 g P, and 105,000 g S, 40 μg of protein was loaded. For 105,000 g P, 10 μg of protein was loaded. The data are representative of at least 3 independent experiments.

    Article Snippet: A cDNA encoding the shorter 316-amino acid version of predicted human RDHE2S (RDHE2Ss) was synthesized and cloned into pCR-Blunt plasmid by Retrogen (San Diego, CA).

    Techniques: Expressing, Western Blot, Northern Blot, Produced, Transfection, Plasmid Preparation, Isolation