Structured Review

TaKaRa genomic pcr
Detection of integration of the expression cassette into DPC genomes with <t>PCR,</t> and detection of protein expression of the introduced genes. (A) PCR detection of the expression cassettes of CDK4, Cyclin D, and TERT in the genomic <t>DNA</t> of DPCs. PCR products from Tuberous sclerosis type 2 (TSC2) were used as a control. (B) Western blot analysis of wild type, K4D, and K4DT cells. The results obtained from CDK4, Cyclin D, and α-tubulin antibodies are shown.
Genomic Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor"

Article Title: Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor

Journal: Frontiers in Cell and Developmental Biology

doi: 10.3389/fcell.2020.00157

Detection of integration of the expression cassette into DPC genomes with PCR, and detection of protein expression of the introduced genes. (A) PCR detection of the expression cassettes of CDK4, Cyclin D, and TERT in the genomic DNA of DPCs. PCR products from Tuberous sclerosis type 2 (TSC2) were used as a control. (B) Western blot analysis of wild type, K4D, and K4DT cells. The results obtained from CDK4, Cyclin D, and α-tubulin antibodies are shown.
Figure Legend Snippet: Detection of integration of the expression cassette into DPC genomes with PCR, and detection of protein expression of the introduced genes. (A) PCR detection of the expression cassettes of CDK4, Cyclin D, and TERT in the genomic DNA of DPCs. PCR products from Tuberous sclerosis type 2 (TSC2) were used as a control. (B) Western blot analysis of wild type, K4D, and K4DT cells. The results obtained from CDK4, Cyclin D, and α-tubulin antibodies are shown.

Techniques Used: Expressing, Polymerase Chain Reaction, Western Blot

2) Product Images from "Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning"

Article Title: Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

Journal: PLoS ONE

doi: 10.1371/journal.pone.0059801

Experimental schemes for vector construction. (A) Composition of the platform vector used for ZFN construction. (B) Construction procedures for ZFN vectors by OLTA. In this figure, construction of a DNA-binding domain composed of 4 ZFs is shown as an example. Three partial ZF fragments were synthesized by the 1st PCR with the primer sets shown in Table 2 and they were combined by overlap extension PCR (2nd PCR) and amplified by 3rd PCR; then the DNA-binding domain was joined with the platform vector by TA cloning. (C) A scheme showing constructs of in vitro transcribed ZFN mRNA and in vivo translated ZFN protein, and ZFN binding with target DNA; Gli3 target site is shown in this figure for example.
Figure Legend Snippet: Experimental schemes for vector construction. (A) Composition of the platform vector used for ZFN construction. (B) Construction procedures for ZFN vectors by OLTA. In this figure, construction of a DNA-binding domain composed of 4 ZFs is shown as an example. Three partial ZF fragments were synthesized by the 1st PCR with the primer sets shown in Table 2 and they were combined by overlap extension PCR (2nd PCR) and amplified by 3rd PCR; then the DNA-binding domain was joined with the platform vector by TA cloning. (C) A scheme showing constructs of in vitro transcribed ZFN mRNA and in vivo translated ZFN protein, and ZFN binding with target DNA; Gli3 target site is shown in this figure for example.

Techniques Used: Plasmid Preparation, Binding Assay, Synthesized, Polymerase Chain Reaction, Amplification, TA Cloning, Construct, In Vitro, In Vivo

The construction and expression of ZFNs. (A) Three-step PCR for the construction of DNA-binding domain of ZFs. PCR products for each PCR step for 6-finger ZF (left-ZF of Rosa26 ), 5-finger ZF (left-ZF of Il2rg ), and 4-finger ZF (left-ZF of Gli3 ) are shown. Arrows indicate the intended base pairs of PCR products. (B) Construction efficiencies of left- and right-ZFNs for 4 target loci. (C, D) The expression of constructed ZFN in mouse zygotes. ZFN mRNA at 200 µg/ml (100 µg/ml each for right- and left-ZFNs) were injected into the cytoplasm of mouse zygotes and after 4 h, ZFN protein expression was confirmed by immunoblotting (C) and immunocytochemistry (D) with anti-Flag antibody. Alpha-tubulin immunoblotting is shown as the internal control, and DNA was stained by propidium iodide.
Figure Legend Snippet: The construction and expression of ZFNs. (A) Three-step PCR for the construction of DNA-binding domain of ZFs. PCR products for each PCR step for 6-finger ZF (left-ZF of Rosa26 ), 5-finger ZF (left-ZF of Il2rg ), and 4-finger ZF (left-ZF of Gli3 ) are shown. Arrows indicate the intended base pairs of PCR products. (B) Construction efficiencies of left- and right-ZFNs for 4 target loci. (C, D) The expression of constructed ZFN in mouse zygotes. ZFN mRNA at 200 µg/ml (100 µg/ml each for right- and left-ZFNs) were injected into the cytoplasm of mouse zygotes and after 4 h, ZFN protein expression was confirmed by immunoblotting (C) and immunocytochemistry (D) with anti-Flag antibody. Alpha-tubulin immunoblotting is shown as the internal control, and DNA was stained by propidium iodide.

Techniques Used: Expressing, Polymerase Chain Reaction, Binding Assay, Construct, Injection, Immunocytochemistry, Staining

3) Product Images from "Generation of CRISPR/Cas9-mediated bicistronic knock-inins1-cre driver mice"

Article Title: Generation of CRISPR/Cas9-mediated bicistronic knock-inins1-cre driver mice

Journal: Experimental Animals

doi: 10.1538/expanim.16-0016

Strategy of bicistronic cre expression in pancreatic beta cells. A: To integrate the 2A-cre sequence just before the stop codon of Ins1 , the 23-nt sequence (5′-CTGGAGAACTACTGCAACTAAGG-3′) containing both PAM and stop codon was chosen as the CRISPR target. The 3′-end of the region of 5′-homology arm (2.0 kb) is the final coding sequence of Ins1 . The 5′-end of the region of 3′-homology arm is the stop codon of Ins1 . The arrows labeled I2AC-GF and I2AC-GR indicate the primers for detecting the knock-in allele. The black box including the white letter P indicates the probe used for Southern blotting. B: PCR products, amplified with the founder genome DNA as the template and the primers I2AC-GF and I2AC-GR, of the appropriate size (2,631 bp) were detected. M: Marker 6 (Nippongene). C: Southern blotting analysis with founder #5 and its offspring (#5-F 1 -1 and #5-F 1 -2). In #5, the knock-in band (arrow) and longer random integration band were detected. In contrast, the KI band and no random integration band were detected in offspring. Lower weight nonspecific bands were detected in all samples.
Figure Legend Snippet: Strategy of bicistronic cre expression in pancreatic beta cells. A: To integrate the 2A-cre sequence just before the stop codon of Ins1 , the 23-nt sequence (5′-CTGGAGAACTACTGCAACTAAGG-3′) containing both PAM and stop codon was chosen as the CRISPR target. The 3′-end of the region of 5′-homology arm (2.0 kb) is the final coding sequence of Ins1 . The 5′-end of the region of 3′-homology arm is the stop codon of Ins1 . The arrows labeled I2AC-GF and I2AC-GR indicate the primers for detecting the knock-in allele. The black box including the white letter P indicates the probe used for Southern blotting. B: PCR products, amplified with the founder genome DNA as the template and the primers I2AC-GF and I2AC-GR, of the appropriate size (2,631 bp) were detected. M: Marker 6 (Nippongene). C: Southern blotting analysis with founder #5 and its offspring (#5-F 1 -1 and #5-F 1 -2). In #5, the knock-in band (arrow) and longer random integration band were detected. In contrast, the KI band and no random integration band were detected in offspring. Lower weight nonspecific bands were detected in all samples.

Techniques Used: Expressing, Sequencing, CRISPR, Labeling, Knock-In, Southern Blot, Polymerase Chain Reaction, Amplification, Marker

Related Articles

Polymerase Chain Reaction:

Article Title: The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
Article Snippet: .. Finally, in order to close the 15 gaps, genomic PCR was carried out for five gaps using an LA long PCR kit (Takara), and 14 BAC clones were retrieved to close the remaining 10 gaps. ..

Article Title: Rapid generation of gene-targeted EPS-derived mouse models through tetraploid complementation
Article Snippet: .. Genomic PCR was performed using PrimeSTAR® HS DNA Polymerase with GC Buffer (Takara, R044B). ..

Article Title: Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning
Article Snippet: .. Genomic PCR of Single Embryo For genome DNA collection, an individual 2-cell embryo was put in 10 µl of Ex Taq buffer (RR001B, TaKaRa), digested with 1 µg/µl of Proteinase K at 60°C for 30 min and heat-inactivated at 95°C for 10 min. .. The embryo lysate solutions were subjected to PCR on the condition of using the primer sets in .

Article Title: A Robust Strategy for Negative Selection of Cre-LoxP Recombination-Based Excision of Transgenes in Induced Pluripotent Stem Cells
Article Snippet: .. Genomic PCR was done with the terra PCR kit from Clontech. ..

Article Title: Generation of CRISPR/Cas9-mediated bicistronic knock-inins1-cre driver mice
Article Snippet: .. Genomic PCR was performed with PrimeSTAR GXL DNA Polymerase® (TAKARA Bio, Shiga, Japan) and the primers ( ). .. PCR products of off-target candidate were sequenced with BigDye Terminator v3.1 Cycle Sequencing Kit and 3500 genetic analyzer (Thermo Fisher Scientific, Massachusetts, U.S.).

Article Title: MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins
Article Snippet: .. Genomic PCR We isolated genomic DNA as previous report ( ) and carried out PCR using PrimeSTAR HS DNA Polymerase (Takara). ..

Article Title: Characterizing HMW-GS alleles of decaploid Agropyron elongatum in relation to evolution and wheat breeding
Article Snippet: .. Genomic PCR was carried out using the LA Taq polymerase (TaKaRa Biotechnology) with GC buffer for GC-rich template. .. The parameters for the reaction were: one cycle at 95°C for 5 min, followed by 30 cycles of 94°C for 40 s, 68°C for 4 min, and a final extension step at 72°C for 7 min. PCR products were separated in 1.0% agarose gels.

Article Title: Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor
Article Snippet: .. Genomic PCR To extract genomic DNA from cells, we used the NucleoSpin Tissue Kit (cat. no. 740952, Takara Bio). .. PCR was performed with 100 ng of template DNA, 1X KOD-FX neo PCR buffer (KFX- 201; Toyobo, Osaka, Japan), 0.4 mM dNTPs (KFX-201, Toyobo), 0.5 U KOD-FX neo (KFX-201, Toyobo), and 0.3 mM of each primer, in accordance with the manufacturer’s protocol.

Clone Assay:

Article Title: The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
Article Snippet: .. Finally, in order to close the 15 gaps, genomic PCR was carried out for five gaps using an LA long PCR kit (Takara), and 14 BAC clones were retrieved to close the remaining 10 gaps. ..

BAC Assay:

Article Title: The Whole-genome Sequencing of the Obligate Intracellular Bacterium Orientia tsutsugamushi Revealed Massive Gene Amplification During Reductive Genome Evolution
Article Snippet: .. Finally, in order to close the 15 gaps, genomic PCR was carried out for five gaps using an LA long PCR kit (Takara), and 14 BAC clones were retrieved to close the remaining 10 gaps. ..

Isolation:

Article Title: MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins
Article Snippet: .. Genomic PCR We isolated genomic DNA as previous report ( ) and carried out PCR using PrimeSTAR HS DNA Polymerase (Takara). ..

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    TaKaRa bcl 2
    Western blot analysis of the protein expressions of Notch, CTNNB1, Bax, <t>Bcl-2,</t> BIM, Hes1, Runx2, and osteocalcin in each transfected group ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each group. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P
    Bcl 2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa arabidopsis tissues
    Expression pattern of EMB1990/YLMG1-1 gene in <t>Arabidopsis</t> tissues and organs. (A) Expression levels of EMB1990/YLMG1-1 genes in different tissues by qPCR assay. R, root; S, stem; L, leave; Sl, seedling; In, inflorescence; F, flower; 1Si, 1 DAP silique; 2Si, 2 DAP silique; 3Si, 3 DAP silique; 4Si, 4 DAP silique; 5Si, 5 DAP silique; 6Si, 6 DAP silique; 7Si, 7 DAP silique. (B–F) GUS activity in pYLMG1-1::GUS transgenic plants. (B) Flower; (C) inflorescence; (D) 7 DAG seedling; (E) 14 DAG seedling; (F) rosette leave. Scale bars = 2mm. (G–J) Fluorescence analysis of embryos at different stage from pYLMG1-1::YLMG1-1-Venus transgenic plants. (G) Globular stage; (H) Heart stage; (G) torpedo stage; (G) bent cotyledon stage. Scale bars = 20 μm.
    Arabidopsis Tissues, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis tissues/product/TaKaRa
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    arabidopsis tissues - by Bioz Stars, 2020-05
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    Image Search Results


    Western blot analysis of the protein expressions of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each group. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: Western blot analysis of the protein expressions of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each group. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Western Blot, Transfection, Expressing

    RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in each transfected group * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Quantitative RT-PCR, Expressing, Transfection

    Immunohistochemistry and positive expression rate of CTNNB1 and Bcl-2 protein in OS and normal bone tissue ( A ) Immunohistochemical images (×200) of CTNNB1 in normal bone and OS tissues. ( B ) Positive expression rate of CTNNB1 in normal bone and OS tissues. ( C ) Immunohistochemical images (×200) of Bcl-2 in normal bone and OS tissues. ( D ) Positive expression rate of Bcl-2 in normal bone and OS tissues; * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: Immunohistochemistry and positive expression rate of CTNNB1 and Bcl-2 protein in OS and normal bone tissue ( A ) Immunohistochemical images (×200) of CTNNB1 in normal bone and OS tissues. ( B ) Positive expression rate of CTNNB1 in normal bone and OS tissues. ( C ) Immunohistochemical images (×200) of Bcl-2 in normal bone and OS tissues. ( D ) Positive expression rate of Bcl-2 in normal bone and OS tissues; * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Immunohistochemistry, Expressing

    RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: RT-qPCR detection of miR-340 expression and relative mRNA expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Quantitative RT-PCR, Expressing

    Western blot analysis of the relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Journal: Bioscience Reports

    Article Title: Up-regulation of microRNA-340 promotes osteosarcoma cell apoptosis while suppressing proliferation, migration, and invasion by inactivating the CTNNB1-mediated Notch signaling pathway

    doi: 10.1042/BSR20171615

    Figure Lengend Snippet: Western blot analysis of the relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin in OS and normal bone tissues ( A ) Relative protein expression of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin. ( B ) Protein bands of Notch, CTNNB1, Bax, Bcl-2, BIM, Hes1, Runx2, and osteocalcin by Western blot analysis; * P

    Article Snippet: The primers of miR-340, Notch, CTNNB1, Bcl-2 associated protein X (Bax), Bcl-2, Bcl-2 interacting mediator of cell death (BIM), hairy and enhancer of split 1 (Hes1), Runt-related transcription factor 2 (Runx2), and osteocalcin were designed and synthesized by Takara (Dalian, Liaoning, China) ( ).

    Techniques: Western Blot, Expressing

    Expression pattern of EMB1990/YLMG1-1 gene in Arabidopsis tissues and organs. (A) Expression levels of EMB1990/YLMG1-1 genes in different tissues by qPCR assay. R, root; S, stem; L, leave; Sl, seedling; In, inflorescence; F, flower; 1Si, 1 DAP silique; 2Si, 2 DAP silique; 3Si, 3 DAP silique; 4Si, 4 DAP silique; 5Si, 5 DAP silique; 6Si, 6 DAP silique; 7Si, 7 DAP silique. (B–F) GUS activity in pYLMG1-1::GUS transgenic plants. (B) Flower; (C) inflorescence; (D) 7 DAG seedling; (E) 14 DAG seedling; (F) rosette leave. Scale bars = 2mm. (G–J) Fluorescence analysis of embryos at different stage from pYLMG1-1::YLMG1-1-Venus transgenic plants. (G) Globular stage; (H) Heart stage; (G) torpedo stage; (G) bent cotyledon stage. Scale bars = 20 μm.

    Journal: Frontiers in Plant Science

    Article Title: Arabidopsis EMB1990 Encoding a Plastid-Targeted YlmG Protein Is Required for Chloroplast Biogenesis and Embryo Development

    doi: 10.3389/fpls.2018.00181

    Figure Lengend Snippet: Expression pattern of EMB1990/YLMG1-1 gene in Arabidopsis tissues and organs. (A) Expression levels of EMB1990/YLMG1-1 genes in different tissues by qPCR assay. R, root; S, stem; L, leave; Sl, seedling; In, inflorescence; F, flower; 1Si, 1 DAP silique; 2Si, 2 DAP silique; 3Si, 3 DAP silique; 4Si, 4 DAP silique; 5Si, 5 DAP silique; 6Si, 6 DAP silique; 7Si, 7 DAP silique. (B–F) GUS activity in pYLMG1-1::GUS transgenic plants. (B) Flower; (C) inflorescence; (D) 7 DAG seedling; (E) 14 DAG seedling; (F) rosette leave. Scale bars = 2mm. (G–J) Fluorescence analysis of embryos at different stage from pYLMG1-1::YLMG1-1-Venus transgenic plants. (G) Globular stage; (H) Heart stage; (G) torpedo stage; (G) bent cotyledon stage. Scale bars = 20 μm.

    Article Snippet: RNA Extraction and RT-PCR Total RNA from different kinds of Arabidopsis tissues was isolated by RNAiso Plus, except the siliques and ovules were extracted using MiniBEST Plant RNA Extraction Kit (TaKaRa, Japan).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Activity Assay, Transgenic Assay, Fluorescence

    Characterization and complementation of Arabidopsis emb1990 mutants. (A) Schematic diagrams of EMB1990/YLMG1-1 gene. The positions of T-DNA insertions in emb1990-1 and emb1990-2 mutants are shown in the schematic diagrams. Arrowheads indicate the positions of primers used for genotyping. (B–F) Seed development in siliques of wild-type, mutants, and complemented plants. White arrows highlight the aborted white ovules, and the siliques were placed as morphological apical to basal from left to right. Bars = 1 mm.

    Journal: Frontiers in Plant Science

    Article Title: Arabidopsis EMB1990 Encoding a Plastid-Targeted YlmG Protein Is Required for Chloroplast Biogenesis and Embryo Development

    doi: 10.3389/fpls.2018.00181

    Figure Lengend Snippet: Characterization and complementation of Arabidopsis emb1990 mutants. (A) Schematic diagrams of EMB1990/YLMG1-1 gene. The positions of T-DNA insertions in emb1990-1 and emb1990-2 mutants are shown in the schematic diagrams. Arrowheads indicate the positions of primers used for genotyping. (B–F) Seed development in siliques of wild-type, mutants, and complemented plants. White arrows highlight the aborted white ovules, and the siliques were placed as morphological apical to basal from left to right. Bars = 1 mm.

    Article Snippet: RNA Extraction and RT-PCR Total RNA from different kinds of Arabidopsis tissues was isolated by RNAiso Plus, except the siliques and ovules were extracted using MiniBEST Plant RNA Extraction Kit (TaKaRa, Japan).

    Techniques: