Journal: bioRxiv

Article Title: The African Swine Fever Virus gene MGF_360-4L inhibits interferon signaling by recruiting mitochondrial selective autophagy receptor SQSTM1 degrading MDA5 antagonizing innate immune responses

doi: 10.1101/2024.09.09.612163

Figure Lengend Snippet: MGF_360-4L is a critical virulence gene of ASFV. (A) Rectal temperatures of pigs were measured daily after intramuscular injection of ASFV-WT (20 HAD 50 ) or ASFV-ΔMGF_360-4L (20 HAD 50 , 100 HAD 50 ) (n=5). (B) Survival rates of pigs were calculated. (C-D) qPCR analysis of ASFV genomic DNA copy number in blood, oral, nasal, and anal swabs obtained from pigs after infection with either ASFV-ΔMGF_360-4L or ASFV-WT. (E) qPCR analysis of ASFV genomic DNA copy number in various organs (heart, liver, spleen, lung, kidney, submandibular lymph nodes, mesenteric lymph nodes, inguinal lymph nodes, and tonsils) from pigs infected with ASFV-ΔMGF_360-4L or ASFV-WT.

Article Snippet: Then, genomic DNA contamination was removed from the RNA template using a gDNA digester (MCE, HY-K0511A) following the manufacturer’s protocol, and reverse transcription was performed to obtain cDNA.

Techniques: Injection, Infection

Journal: bioRxiv

Article Title: Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth

doi: 10.1101/2024.09.03.611075

Figure Lengend Snippet: (A) The sgRNA target site on CrCTI1 gene. Black arrow boxes with number stand for exons, and the black line represent the introns. (B) Genomic DNA (gDNA) PCR verification of cassette insertion in the crcti1 mutants. (C) Sanger sequencing results. (D) RT-PCR analyses of CrCTI1 expression in WT and the three crcti1 mutants. RACK1 , receptor for activated kinase C 1 was used as reference gene. M, marker; WT, wild type; PAM: protospacer adjacent motif.

Article Snippet: To remove potential genomic DNA contaminations, the total RNA extracts were incubated with TURBO TM DNase (Life technologies).

Techniques: Sequencing, Reverse Transcription Polymerase Chain Reaction, Expressing, Marker