genomic dna cfdna 10 ng  (qiagen)


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    qiagen genomic dna cfdna 10 ng
    Overview of copy number variations via plasma cell-free <t>DNA</t> analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.
    Genomic Dna Cfdna 10 Ng, supplied by qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna cfdna 10 ng/product/qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna cfdna 10 ng - by Bioz Stars, 2021-05
    86/100 stars

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    1) Product Images from "Non-invasive detection of biliary tract cancer by low-coverage whole genome sequencing from plasma cell-free DNA: A prospective cohort study"

    Article Title: Non-invasive detection of biliary tract cancer by low-coverage whole genome sequencing from plasma cell-free DNA: A prospective cohort study

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2020.100908

    Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.
    Figure Legend Snippet: Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.

    Techniques Used:

    Related Articles

    Incubation:

    Article Title: Molecular Specificity, Convergence and Constraint Shape Adaptive Evolution in Nutrient-Poor Environments
    Article Snippet: 1 µg of gDNA sample was then sonicated in a Covaris AFA to obtain fragments of 300–500 bp. .. To blunt the ends of fragmented gDNA we incubated with PNK (10 Unit) and T4 DNA polymerase (12 unit) at 20°C for 30 min, and then purified using QIAGEN Min-Elute Columns. .. Adenosine overhangs were added to the blunted DNA using Exo(-) Klenow (15 Unit) incubated at 37°C for 20 minutes, followed by purification using QIAGEN Min-Elute Column and elution in 19 µL EB buffer.

    Purification:

    Article Title: Molecular Specificity, Convergence and Constraint Shape Adaptive Evolution in Nutrient-Poor Environments
    Article Snippet: 1 µg of gDNA sample was then sonicated in a Covaris AFA to obtain fragments of 300–500 bp. .. To blunt the ends of fragmented gDNA we incubated with PNK (10 Unit) and T4 DNA polymerase (12 unit) at 20°C for 30 min, and then purified using QIAGEN Min-Elute Columns. .. Adenosine overhangs were added to the blunted DNA using Exo(-) Klenow (15 Unit) incubated at 37°C for 20 minutes, followed by purification using QIAGEN Min-Elute Column and elution in 19 µL EB buffer.

    Article Title: Adaptation, Ecology, and Evolution of the Halophilic Stromatolite Archaeon Halococcus hamelinensis Inferred through Genome Analyses
    Article Snippet: Sample quality and quantity were assessed prior to library preparation, as described in the GS FLX Titanium General Library Preparation Method Manual (Roche). .. Briefly, 8 μ g of gDNA was fragmented by nebulization, fragmented DNA run on a 0.8% agarose gel and fragments between ~500 and 800 bp excised, and purified using a QiaQuick Gel Extraction Kit (Qiagen). .. DNA sample quality assessment, fragment end repair and adaptor ligation, small fragment removal, library immobilization, and single-stranded DNA library isolation were performed as per GS FLX Titanium Sequencing Method Manual (Roche).

    Agarose Gel Electrophoresis:

    Article Title: Adaptation, Ecology, and Evolution of the Halophilic Stromatolite Archaeon Halococcus hamelinensis Inferred through Genome Analyses
    Article Snippet: Sample quality and quantity were assessed prior to library preparation, as described in the GS FLX Titanium General Library Preparation Method Manual (Roche). .. Briefly, 8 μ g of gDNA was fragmented by nebulization, fragmented DNA run on a 0.8% agarose gel and fragments between ~500 and 800 bp excised, and purified using a QiaQuick Gel Extraction Kit (Qiagen). .. DNA sample quality assessment, fragment end repair and adaptor ligation, small fragment removal, library immobilization, and single-stranded DNA library isolation were performed as per GS FLX Titanium Sequencing Method Manual (Roche).

    Gel Extraction:

    Article Title: Adaptation, Ecology, and Evolution of the Halophilic Stromatolite Archaeon Halococcus hamelinensis Inferred through Genome Analyses
    Article Snippet: Sample quality and quantity were assessed prior to library preparation, as described in the GS FLX Titanium General Library Preparation Method Manual (Roche). .. Briefly, 8 μ g of gDNA was fragmented by nebulization, fragmented DNA run on a 0.8% agarose gel and fragments between ~500 and 800 bp excised, and purified using a QiaQuick Gel Extraction Kit (Qiagen). .. DNA sample quality assessment, fragment end repair and adaptor ligation, small fragment removal, library immobilization, and single-stranded DNA library isolation were performed as per GS FLX Titanium Sequencing Method Manual (Roche).

    Sequencing:

    Article Title: Non-invasive detection of biliary tract cancer by low-coverage whole genome sequencing from plasma cell-free DNA: A prospective cohort study
    Article Snippet: .. DNA was fragmented into an average size of 300 bp (cfDNA without fragmentation), and then 100 ng of fragmented genomic DNA (cfDNA 10 ng) was used for the preparation of sequencing libraries (NEBnext Ultra II). .. 8 bp barcoded sequencing adaptors were then ligated with DNA fragments and amplified by PCR.

    Isolation:

    Article Title: Investigation of Plasma cell‐free cancer genome chromosomal instability as a tool for targeted minimally invasive biomarkers for primary liver cancer diagnoses, et al. Investigation of Plasma cell‐free cancer genome chromosomal instability as a tool for targeted minimally invasive biomarkers for primary liver cancer diagnoses
    Article Snippet: .. 2.2 Next‐generation sequencingTotal genomic DNA and cfDNA were isolated from tissue samples and plasma using the Amp Genomic DNA Kit (TIANGEN) and QIAseq cfDNA Extraction kit (Qiagen), respectively. .. Next‐generation sequencing was performed as previously described., DNA was fragmented into an average size of 300bp (cfDNA without fragmentation) and, then, 100 ng of fragmented genomic DNA (cfDNA 10 ng) was used for preparation of sequencing libraries (NEBnext Ultra II).

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  • 86
    qiagen genomic dna cfdna 10 ng
    Overview of copy number variations via plasma cell-free <t>DNA</t> analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.
    Genomic Dna Cfdna 10 Ng, supplied by qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna cfdna 10 ng/product/qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna cfdna 10 ng - by Bioz Stars, 2021-05
    86/100 stars
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    Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.

    Journal: Translational Oncology

    Article Title: Non-invasive detection of biliary tract cancer by low-coverage whole genome sequencing from plasma cell-free DNA: A prospective cohort study

    doi: 10.1016/j.tranon.2020.100908

    Figure Lengend Snippet: Overview of copy number variations via plasma cell-free DNA analysis in the included patients. (A) copy number variation genome of gallbladder cancer. (B) copy number variation genome of cholangiocarcinoma. (C) Copy number changes of benign biliary lesions. (D) heatmap of copy number variation quantified by chromosome Z-scores for all patients. GC, gallbladder cancer; CC, cholangiocarcinoma; BE, benign lesions.

    Article Snippet: DNA was fragmented into an average size of 300 bp (cfDNA without fragmentation), and then 100 ng of fragmented genomic DNA (cfDNA 10 ng) was used for the preparation of sequencing libraries (NEBnext Ultra II).

    Techniques: