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a baumannii atcc 19606 whole genome sequence  (ATCC)
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ATCC a baumannii atcc 19606 whole genome sequence
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
A Baumannii Atcc 19606 Whole Genome Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microbial community surveys  (Broad Clinical Labs)
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Broad Clinical Labs microbial community surveys
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
Microbial Community Surveys, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genome sequence  (Biotechnology Information)
90
Biotechnology Information genome sequence
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
Genome Sequence, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genome sequence - by Bioz Stars, 2026-03
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genome shotgun sequencing wgs  (Broad Clinical Labs)
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Broad Clinical Labs genome shotgun sequencing wgs
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
Genome Shotgun Sequencing Wgs, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genome shotgun sequencing wgs - by Bioz Stars, 2026-03
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marker 12 whole genome sequencing  (Broad Clinical Labs)
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Broad Clinical Labs marker 12 whole genome sequencing
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
Marker 12 Whole Genome Sequencing, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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marker 12 whole genome sequencing - by Bioz Stars, 2026-03
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nanopore genome sequencing  (ATCC)
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ATCC nanopore genome sequencing
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
Nanopore Genome Sequencing, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nanopore genome sequencing - by Bioz Stars, 2026-03
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whole genome sequencing  (Broad Clinical Labs)
96
Broad Clinical Labs whole genome sequencing
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
Whole Genome Sequencing, supplied by Broad Clinical Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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whole genome sequencing - by Bioz Stars, 2026-03
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v natriegens atcc 14048 reference genome sequence  (ATCC)
98
ATCC v natriegens atcc 14048 reference genome sequence
A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii <t>ATCC</t> <t>19606</t> were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.
V Natriegens Atcc 14048 Reference Genome Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii ATCC 19606 were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.

Journal: bioRxiv

Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

doi: 10.64898/2026.02.02.703249

Figure Lengend Snippet: A. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were stained with DiSC3(5), treated with either 1×MIC of Bald’s eyesalve (purple) or water (black), and their fluorescence monitored for 1 h. There was increased DiSC3(5) fluorescence, indicating membrane depolarisation in samples treated with Bald’s eyesalve. The red arrow indicate point of treatment. Each line represents the mean of three technical replicates. B. S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were treated with 1×MIC of Bald’s eyesalve (purple) or water (grey) for 1 h, stained with propidium iodide, and fluorescence was measured after 1 h. Each symbol represents an individual bacterial culture; box plots show the fluorescence intensity (in RFU) of propidium iodide. C. S. aureus Newman and A. baumannii ATCC 19606 were treated with 2×MIC of Bald’s eyesalve or water for 2 h, stained with FM4-64, and then imaged with a widefield fluorescence microscope at oil immersion lens (x100) magnification. Increased FM4-64 fluorescence in S. aureus and the presence of dense spots of fluorescence in A. baumannii suggest membrane perturbation.

Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

Techniques: Staining, Fluorescence, Membrane, Microscopy

Both A. baumannii ATCC 19606 and S. aureus Newman were treated with 2×MIC of Bald’s eyesalve or water for 2 h and imaged with a scanning electron microscope.

Journal: bioRxiv

Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

doi: 10.64898/2026.02.02.703249

Figure Lengend Snippet: Both A. baumannii ATCC 19606 and S. aureus Newman were treated with 2×MIC of Bald’s eyesalve or water for 2 h and imaged with a scanning electron microscope.

Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

Techniques: Microscopy

Significantly differentially expressed genes were defined as those with p-values ≤ 0.05 and |log2foldchange| ≥1.5 in treated vs. untreated samples; n = 3 and 4 cultures per treatment for A. baumannii ATCC 19606 and S. aureus Newman, respectively.

Journal: bioRxiv

Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

doi: 10.64898/2026.02.02.703249

Figure Lengend Snippet: Significantly differentially expressed genes were defined as those with p-values ≤ 0.05 and |log2foldchange| ≥1.5 in treated vs. untreated samples; n = 3 and 4 cultures per treatment for A. baumannii ATCC 19606 and S. aureus Newman, respectively.

Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

Techniques:

A. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in S. aureus Newman in response to Bald’s eyesalve. Red dashed lines indicate |log2-fold change| = 1.5, n = 4 cultures per treatment. B. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in A. baumannii ATCC 19606 in response to Bald’s eyesalve. Red dashed line indicates log2-fold change = - 1.5, n = 3 cultures per treatment.

Journal: bioRxiv

Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

doi: 10.64898/2026.02.02.703249

Figure Lengend Snippet: A. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in S. aureus Newman in response to Bald’s eyesalve. Red dashed lines indicate |log2-fold change| = 1.5, n = 4 cultures per treatment. B. Expression profile of significantly differentially expressed (p < 0.05) biofilm-associated genes in A. baumannii ATCC 19606 in response to Bald’s eyesalve. Red dashed line indicates log2-fold change = - 1.5, n = 3 cultures per treatment.

Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

Techniques: Expressing

S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were subjected to subinhibitory concentrations of Bald’s eyesalve or antibiotic and subsequently passaged in different concentrations based on the previous day’s highest concentration with growth. This was done for 14 days. The graph’s axes show the highest of two tested concentrations in which cultures grew at each time point, expressed as a multiple of the ancestral MIC.

Journal: bioRxiv

Article Title: Uncovering the multifaceted mechanism of action of a historical antimicrobial

doi: 10.64898/2026.02.02.703249

Figure Lengend Snippet: S. aureus Newman, A. baumannii ATCC 19606, and P. aeruginosa PA14 were subjected to subinhibitory concentrations of Bald’s eyesalve or antibiotic and subsequently passaged in different concentrations based on the previous day’s highest concentration with growth. This was done for 14 days. The graph’s axes show the highest of two tested concentrations in which cultures grew at each time point, expressed as a multiple of the ancestral MIC.

Article Snippet: For S. aureus , all reads were aligned to S. aureus Newman whole genome sequence (accession number - NC009641.1 , assembly - GCF_000010465.1), while A. baumannii reads were aligned to A. baumannii ATCC 19606 whole genome sequence (accession number - NZ_CP046654.1, assembly - GCF_009759685.1).

Techniques: Concentration Assay