genetic analyzer  (Thermo Fisher)


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    Name:
    Genetic Analyzer Sample Tubes
    Description:
    These are sample tubes for use in the 48 sample tray of the 310 Genetic Analyzer Use with 1 0 mL Glass Syringe 310 Genetic Analyzer Capillary 47 cm 36 cm wtr 310 Running Buffer 10X BigDye Terminator v1 1 Cycle Sequencing Kit GeneScan 500 ROX Size Standard Hi Di Formamide MicroAmp Reaction Tube with Cap 0 2 ml POP 4 Polymer for the 310 Genetic Analyzer POP 6 Polymer for the 310 Genetic Analyzer Septa for 0 5 ml Sample TubesFor Research Use Only Not intended for human or animal therapeutic or diagnostic use
    Catalog Number:
    401957
    Price:
    None
    Applications:
    Sanger Sequencing|Sanger Sequencing Technology & Accessories|Sequencing
    Category:
    Lab Supplies Plastics Glassware
    Buy from Supplier


    Structured Review

    Thermo Fisher genetic analyzer
    These are sample tubes for use in the 48 sample tray of the 310 Genetic Analyzer Use with 1 0 mL Glass Syringe 310 Genetic Analyzer Capillary 47 cm 36 cm wtr 310 Running Buffer 10X BigDye Terminator v1 1 Cycle Sequencing Kit GeneScan 500 ROX Size Standard Hi Di Formamide MicroAmp Reaction Tube with Cap 0 2 ml POP 4 Polymer for the 310 Genetic Analyzer POP 6 Polymer for the 310 Genetic Analyzer Septa for 0 5 ml Sample TubesFor Research Use Only Not intended for human or animal therapeutic or diagnostic use
    https://www.bioz.com/result/genetic analyzer/product/Thermo Fisher
    Average 95 stars, based on 712 article reviews
    Price from $9.99 to $1999.99
    genetic analyzer - by Bioz Stars, 2020-07
    95/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: The molecular spectrum and distribution of haemoglobinopathies in Cyprus: a 20-year retrospective study
    Article Snippet: .. When no mutation was detected by the ARMS-PCR, the β-globin gene was examined by performing PCR and Sanger sequencing using an ABI 3130xl Genetic Analyzer (Applied Biosystems-Life Technologies, USA). .. Deletion-type mutations were tested using the multiplex ligation-dependent probe amplification method (MLPA) by utilising the SALSA MLPA probemix P102 HBB protocol (MRC, Holland).

    Labeling:

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: .. After purification with the QIAquick kit (QIAGEN) and labeling with the Big Dye Terminator (Life Technologies), DNA was sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystem). .. GSTM3 *A and GSTM3*B alleles A 202-bp PCR fragment was amplified using primers 5′-GCTACCTGGACAACTGAAAC-3′ and 5′-CGGTTCTGATCCAAGATATC-3′ and the following program: 95 °C 5 min, then (95 °C 30 s, 56 °C 30 s, 72 °C 1 min) for 25 cycles and 72 °C 15 min. PCR products were analyzed using an ABI 3130xl Genetic Analyzer (Applied Biosystem) and their size measured with the Gene Mapper software (Applied Biosystem).

    Purification:

    Article Title: Causative agent of canine heartworm (Dirofilaria immitis) detected in wild lemurs
    Article Snippet: .. Purified products were then sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystems) and resulting sequence reads underwent BLAST analysis. ..

    Article Title: Does DNA Methylation of PPARGC1A Influence Insulin Action in First Degree Relatives of Patients with Type 2 Diabetes?
    Article Snippet: .. DNA samples were precipitated with the BigDye XTerminator Purification Kit (Applied Biosystems), and the samples were sequenced in an ABI 3130xl Genetic Analyzer (Applied Biosystems). .. The sequence trace files were subjected to quality control and methylation quantification using the epigenetic sequencing methylation (ESME) analysis software version 3.2.1 (Epigenomics, Berlin, Germany).

    Article Title: DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis
    Article Snippet: .. After purification with the QIAquick kit (QIAGEN) and labeling with the Big Dye Terminator (Life Technologies), DNA was sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystem). .. GSTM3 *A and GSTM3*B alleles A 202-bp PCR fragment was amplified using primers 5′-GCTACCTGGACAACTGAAAC-3′ and 5′-CGGTTCTGATCCAAGATATC-3′ and the following program: 95 °C 5 min, then (95 °C 30 s, 56 °C 30 s, 72 °C 1 min) for 25 cycles and 72 °C 15 min. PCR products were analyzed using an ABI 3130xl Genetic Analyzer (Applied Biosystem) and their size measured with the Gene Mapper software (Applied Biosystem).

    Electrophoresis:

    Article Title: Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms ▿ Population Dynamics in Pigs on Farrow-to-Finish Farms ▿ †
    Article Snippet: .. One microliter of the final product was mixed with 8.6 μl of deionized formamide and 0.4 μl of the internal lane standard (Gene Scan-600 LIZ size standard; Applied Biosystems) and analyzed by means of capillary electrophoresis using an ABI 3130xl Genetic Analyzer (Applied Biosystems). .. AFLP profiles were collected with the data collection software 3.0 (Applied Biosystems).

    Article Title: A novel IGH@ gene rearrangement associated with CDKN2A/B deletion in young adult B-cell acute lymphoblastic leukemia
    Article Snippet: .. Amplified probes and Genescan 500 ROX Size Standard were separated using capillary electrophoresis using a 4-capillary ABI-PRISM 3130xl Genetic Analyzer (Applied Biosystems; Thermo Fisher Scientific, Inc.). .. Sizing of peaks and quantification of peak areas and heights was performed using GeneMarker software version 1.9 (SoftGenetics, LLC., State College, PA, USA).

    Sequencing:

    Article Title: Causative agent of canine heartworm (Dirofilaria immitis) detected in wild lemurs
    Article Snippet: .. Purified products were then sequenced using an ABI 3130xl Genetic Analyzer (Applied Biosystems) and resulting sequence reads underwent BLAST analysis. ..

    Article Title: Genetic turnovers and northern survival during the last glacial maximum in European brown bears, et al. Genetic turnovers and northern survival during the last glacial maximum in European brown bears
    Article Snippet: .. Final sequencing was performed on an ABI 3130xl Genetic Analyzer (Applied Biosystems Inc.). .. 2.2 Radiocarbon dating and stable isotope analysis A subset of 72 brown bear samples that produced PCR products for the two fragments and contained enough material were resampled for collagen.

    Article Title: The molecular spectrum and distribution of haemoglobinopathies in Cyprus: a 20-year retrospective study
    Article Snippet: .. When no mutation was detected by the ARMS-PCR, the β-globin gene was examined by performing PCR and Sanger sequencing using an ABI 3130xl Genetic Analyzer (Applied Biosystems-Life Technologies, USA). .. Deletion-type mutations were tested using the multiplex ligation-dependent probe amplification method (MLPA) by utilising the SALSA MLPA probemix P102 HBB protocol (MRC, Holland).

    Polymerase Chain Reaction:

    Article Title: The molecular spectrum and distribution of haemoglobinopathies in Cyprus: a 20-year retrospective study
    Article Snippet: .. When no mutation was detected by the ARMS-PCR, the β-globin gene was examined by performing PCR and Sanger sequencing using an ABI 3130xl Genetic Analyzer (Applied Biosystems-Life Technologies, USA). .. Deletion-type mutations were tested using the multiplex ligation-dependent probe amplification method (MLPA) by utilising the SALSA MLPA probemix P102 HBB protocol (MRC, Holland).

    Article Title: Detection of large expansions in myotonic dystrophy type 1 using triplet primed PCR
    Article Snippet: .. PCR PRODUCT ANALYSIS ON GENETIC ANALYZER Products were separated on an ABI PRISM 3130 × l genetic analyzer (Life Tech, Grand Island, NY 14072, USA). .. 1 μl of PCR product was mixed with 0.5 μl of MapMarker ROX 1000 (Bioventures, Murfreesboro, TN, USA) and 9 μl of HiDi Formamide (Life Tech, Grand Island, NY 14072, USA).

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  • 99
    Thermo Fisher abi 3130 genetic analyzer
    Characterization of SMA haplotypes. (A) To characterize the extent of SMN1 deletions, we performed PCR on loci that mapped to multiple sites in the region. By selecting amplicons which exhibited sequence changes between loci, we were able to assess the presence or absence of each locus after PCR amplification and Sanger sequencing. Green blocks represent loci that were present, and red blocks denote deleted loci. The samples (i.e. haplotype combinations) are listed on the left side of the figure. (B) Competitive PCR was used to calculate SMN2 copy number in our patient cohort. Samples were subjected to multiplex PCR with limiting deoxynucleotide triphosphates and the resulting amplicons were size-fractionated on an <t>ABI</t> 3130 Genetic Analyzer. Samples with greater SMN2 copy number demonstrated increased generation of SMN2 -specific product versus an internal control locus (albumin gene, ALB ). (C) The area under the curve for each amplicon, as provided by the Sequencing Analysis software, was used to calculate the ratio of SMN2 -specific product to ALB product. For SMN2 copy number from 1–4, three separate samples were PCR amplified and analyzed, and the SMN2/ALB ratios were highly correlated with SMN2 copy number.
    Abi 3130 Genetic Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 3130 genetic analyzer/product/Thermo Fisher
    Average 99 stars, based on 280 article reviews
    Price from $9.99 to $1999.99
    abi 3130 genetic analyzer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher abi 3500 genetic analyzer
    Amplification products of a male (A) and a female (B), detected by an <t>ABI</t> 3500 genetic analyzer.
    Abi 3500 Genetic Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi 3500 genetic analyzer/product/Thermo Fisher
    Average 94 stars, based on 832 article reviews
    Price from $9.99 to $1999.99
    abi 3500 genetic analyzer - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of SMA haplotypes. (A) To characterize the extent of SMN1 deletions, we performed PCR on loci that mapped to multiple sites in the region. By selecting amplicons which exhibited sequence changes between loci, we were able to assess the presence or absence of each locus after PCR amplification and Sanger sequencing. Green blocks represent loci that were present, and red blocks denote deleted loci. The samples (i.e. haplotype combinations) are listed on the left side of the figure. (B) Competitive PCR was used to calculate SMN2 copy number in our patient cohort. Samples were subjected to multiplex PCR with limiting deoxynucleotide triphosphates and the resulting amplicons were size-fractionated on an ABI 3130 Genetic Analyzer. Samples with greater SMN2 copy number demonstrated increased generation of SMN2 -specific product versus an internal control locus (albumin gene, ALB ). (C) The area under the curve for each amplicon, as provided by the Sequencing Analysis software, was used to calculate the ratio of SMN2 -specific product to ALB product. For SMN2 copy number from 1–4, three separate samples were PCR amplified and analyzed, and the SMN2/ALB ratios were highly correlated with SMN2 copy number.

    Journal: PLoS ONE

    Article Title: Spinal muscular atrophy within Amish and Mennonite populations: Ancestral haplotypes and natural history

    doi: 10.1371/journal.pone.0202104

    Figure Lengend Snippet: Characterization of SMA haplotypes. (A) To characterize the extent of SMN1 deletions, we performed PCR on loci that mapped to multiple sites in the region. By selecting amplicons which exhibited sequence changes between loci, we were able to assess the presence or absence of each locus after PCR amplification and Sanger sequencing. Green blocks represent loci that were present, and red blocks denote deleted loci. The samples (i.e. haplotype combinations) are listed on the left side of the figure. (B) Competitive PCR was used to calculate SMN2 copy number in our patient cohort. Samples were subjected to multiplex PCR with limiting deoxynucleotide triphosphates and the resulting amplicons were size-fractionated on an ABI 3130 Genetic Analyzer. Samples with greater SMN2 copy number demonstrated increased generation of SMN2 -specific product versus an internal control locus (albumin gene, ALB ). (C) The area under the curve for each amplicon, as provided by the Sequencing Analysis software, was used to calculate the ratio of SMN2 -specific product to ALB product. For SMN2 copy number from 1–4, three separate samples were PCR amplified and analyzed, and the SMN2/ALB ratios were highly correlated with SMN2 copy number.

    Article Snippet: Extension products were size-fractionated on an ABI 3130 Genetic Analyzer and analyzed using Sequencing Analysis software (ThermoFisher Scientific, Waltham, MA).

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification, Multiplex Assay, Software

    Amplification products of a male (A) and a female (B), detected by an ABI 3500 genetic analyzer.

    Journal: PeerJ

    Article Title: Genome-based development of 15 microsatellite markers in fluorescent multiplexes for parentage testing in captive tigers

    doi: 10.7717/peerj.8939

    Figure Lengend Snippet: Amplification products of a male (A) and a female (B), detected by an ABI 3500 genetic analyzer.

    Article Snippet: The electrophoretic separations were performed on an ABI 3500 Genetic Analyzer (Thermo Fisher, Waltham, MA, USA).

    Techniques: Amplification

    Typical chromatograms of the individuall alleles as obtained using ABI3500.

    Journal: PLoS ONE

    Article Title: Merle phenotypes in dogs – SILV SINE insertions from Mc to Mh

    doi: 10.1371/journal.pone.0198536

    Figure Lengend Snippet: Typical chromatograms of the individuall alleles as obtained using ABI3500.

    Article Snippet: Refined SILV SINE genotyping SILV SINE fragments were separated in denaturing polyacrylamide gel on ABI3500 Genetic Analyzer to obtain the highest resolution possible for fragment analysis technology.

    Techniques:

    Electropherogram of Control DNA 007 (1 ng) amplified by Huaxia Platinum System. Control DNA 007 was amplified following the recommended protocol (27 cycles). Amplified product was separated on an Applied Biosystems 3500 Genetic Analyzer. Panel labeled “Yin…” is Y-InDel. Panel labeled “AM…” is Amelogenin.

    Journal: Scientific Reports

    Article Title: Developmental Validation of the Huaxia Platinum System and application in 3 main ethnic groups of China

    doi: 10.1038/srep31075

    Figure Lengend Snippet: Electropherogram of Control DNA 007 (1 ng) amplified by Huaxia Platinum System. Control DNA 007 was amplified following the recommended protocol (27 cycles). Amplified product was separated on an Applied Biosystems 3500 Genetic Analyzer. Panel labeled “Yin…” is Y-InDel. Panel labeled “AM…” is Amelogenin.

    Article Snippet: In this study, serial dilutions of the Control DNA 007 were analyzed on an Applied Biosystems 3500 Genetic Analyzer (Thermo Fisher Scientific).

    Techniques: Amplification, Labeling