genescan analysis system  (Thermo Fisher)


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    Thermo Fisher genescan analysis system
    Genescan Analysis System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genescan analysis system/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genescan analysis system - by Bioz Stars, 2020-07
    85/100 stars

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    Primer Extension Assay:

    Article Title: Regulation of Swarming Motility and flhDCSm Expression by RssAB Signaling in Serratia marcescens
    Article Snippet: .. The transcriptional start sites were identified by a primer extension assay using a 6-carboxyfluorescein (FAM)-labeled primer and the GeneScan analysis system (Applied Biosystems) described by Lloyd et al. ( ) with some modifications. .. Briefly, purified RNA was reverse transcribed into cDNA with ImProm-II reverse transcriptase (Promega) by use of primer FlhD-PE to form FAM-labeled cDNA (Table ).

    other:

    Article Title: Myasthenic syndrome caused by mutation of the SCN4A sodium channel
    Article Snippet: We also examined (CT)n dinucleotide repeat in intron 22 and (CT)n(CA)n dinucleotide repeats in intron 23 ( ) by the GeneScan Analysis System (Applied Biosystems).

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    Thermo Fisher genescan 1200 liz size standard
    Genescan 1200 Liz Size Standard, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genescan 1200 liz size standard/product/Thermo Fisher
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    genescan 1200 liz size standard - by Bioz Stars, 2020-07
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    91
    Thermo Fisher genescan analysis
    Mutation variants identified in CD34 + and CD34 - AML blast subsets and their allelic frequency. ( a ) FACS analysis shows blast cells derived from the same AML patient at diagnosis and relapse, and sorted to CD34 + CD117 + and CD34 - CD117 + fractions. DNA was extracted from each fraction and PCR products were examined by genetic analyzer for FLT3-IDT mutation. Mutant FLT3-ITD (right column) and wt- FLT3 (left column) alleles are shown in each CD34 compartment. ( b ) BM blasts from 4 AML patients were sorted into CD34 + CD117 + and CD34 - CD117 + fractions. Genomic DNA was extracted from each subset; targeted sequencing conducted using PGM Ion Torrent, qualified by coverage of ~2000 reads, was performed for: NPM1 and DNMT3A mutations. All the 4 patients carried NPM1 mut (TCTG insertion) and FLT3-ITD mutations. In the DNMT3A hotspot region, two variants were identified: R882H and R882C. The allelic frequency of the variants in CD34 + and CD34 - subpopulations is presented for each patient. The calculated allelic ratio (AR) for FLT3-ITD mutation (detected using the <t>Genescan</t> method) in each fraction is presented, when available. The circles, denoting cells, represent the ratio between the numbers of blast cells in each fraction.
    Genescan Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genescan analysis/product/Thermo Fisher
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    genescan analysis - by Bioz Stars, 2020-07
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    99
    Thermo Fisher genescan 500 liz internal size standard
    Mutation variants identified in CD34 + and CD34 - AML blast subsets and their allelic frequency. ( a ) FACS analysis shows blast cells derived from the same AML patient at diagnosis and relapse, and sorted to CD34 + CD117 + and CD34 - CD117 + fractions. DNA was extracted from each fraction and PCR products were examined by genetic analyzer for FLT3-IDT mutation. Mutant FLT3-ITD (right column) and wt- FLT3 (left column) alleles are shown in each CD34 compartment. ( b ) BM blasts from 4 AML patients were sorted into CD34 + CD117 + and CD34 - CD117 + fractions. Genomic DNA was extracted from each subset; targeted sequencing conducted using PGM Ion Torrent, qualified by coverage of ~2000 reads, was performed for: NPM1 and DNMT3A mutations. All the 4 patients carried NPM1 mut (TCTG insertion) and FLT3-ITD mutations. In the DNMT3A hotspot region, two variants were identified: R882H and R882C. The allelic frequency of the variants in CD34 + and CD34 - subpopulations is presented for each patient. The calculated allelic ratio (AR) for FLT3-ITD mutation (detected using the <t>Genescan</t> method) in each fraction is presented, when available. The circles, denoting cells, represent the ratio between the numbers of blast cells in each fraction.
    Genescan 500 Liz Internal Size Standard, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genescan 500 liz internal size standard/product/Thermo Fisher
    Average 99 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    genescan 500 liz internal size standard - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    Mutation variants identified in CD34 + and CD34 - AML blast subsets and their allelic frequency. ( a ) FACS analysis shows blast cells derived from the same AML patient at diagnosis and relapse, and sorted to CD34 + CD117 + and CD34 - CD117 + fractions. DNA was extracted from each fraction and PCR products were examined by genetic analyzer for FLT3-IDT mutation. Mutant FLT3-ITD (right column) and wt- FLT3 (left column) alleles are shown in each CD34 compartment. ( b ) BM blasts from 4 AML patients were sorted into CD34 + CD117 + and CD34 - CD117 + fractions. Genomic DNA was extracted from each subset; targeted sequencing conducted using PGM Ion Torrent, qualified by coverage of ~2000 reads, was performed for: NPM1 and DNMT3A mutations. All the 4 patients carried NPM1 mut (TCTG insertion) and FLT3-ITD mutations. In the DNMT3A hotspot region, two variants were identified: R882H and R882C. The allelic frequency of the variants in CD34 + and CD34 - subpopulations is presented for each patient. The calculated allelic ratio (AR) for FLT3-ITD mutation (detected using the Genescan method) in each fraction is presented, when available. The circles, denoting cells, represent the ratio between the numbers of blast cells in each fraction.

    Journal: Scientific Reports

    Article Title: ERK Activity in Immature Leukemic Cells Drives Clonal Selection during Induction Therapy for Acute Myeloid Leukemia

    doi: 10.1038/s41598-020-65061-6

    Figure Lengend Snippet: Mutation variants identified in CD34 + and CD34 - AML blast subsets and their allelic frequency. ( a ) FACS analysis shows blast cells derived from the same AML patient at diagnosis and relapse, and sorted to CD34 + CD117 + and CD34 - CD117 + fractions. DNA was extracted from each fraction and PCR products were examined by genetic analyzer for FLT3-IDT mutation. Mutant FLT3-ITD (right column) and wt- FLT3 (left column) alleles are shown in each CD34 compartment. ( b ) BM blasts from 4 AML patients were sorted into CD34 + CD117 + and CD34 - CD117 + fractions. Genomic DNA was extracted from each subset; targeted sequencing conducted using PGM Ion Torrent, qualified by coverage of ~2000 reads, was performed for: NPM1 and DNMT3A mutations. All the 4 patients carried NPM1 mut (TCTG insertion) and FLT3-ITD mutations. In the DNMT3A hotspot region, two variants were identified: R882H and R882C. The allelic frequency of the variants in CD34 + and CD34 - subpopulations is presented for each patient. The calculated allelic ratio (AR) for FLT3-ITD mutation (detected using the Genescan method) in each fraction is presented, when available. The circles, denoting cells, represent the ratio between the numbers of blast cells in each fraction.

    Article Snippet: GeneScan analysis of FLT3-ITD mutation Identification of FLT3-ITD mutant allele in sorted AML blast samples was performed by PCR with specific oligos for exon 11, labeled with 5-FAM, and with a reverse primer for exon 12 (Table ).

    Techniques: Mutagenesis, FACS, Derivative Assay, Polymerase Chain Reaction, Sequencing