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Bio-Rad genepulser xcell
Genepulser Xcell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genepulser xcell/product/Bio-Rad
Average 92 stars, based on 55 article reviews
Price from $9.99 to $1999.99
genepulser xcell - by Bioz Stars, 2020-08
92/100 stars

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Electroporation:

Article Title: RIG-I and MDA-5 Detection of Viral RNA-dependent RNA Polymerase Activity Restricts Positive-Strand RNA Virus Replication
Article Snippet: .. RD cells (0.2×106 per 60-mm dish) were transfected with DNA (0.2 µg/ml) by electroporation in GenePulser Xcell (Bio-Rad) instrument (settings: Exponential Wave, 190 V; 975 microfarads [µF]) in 400 µl OptiMEM medium (Invitrogen). .. RNA (2 µg/ml) and DNA (10 µg/ml) into MEF cells (0.25×106 per 60-mm dish) were transfected by electroporation (Exponential Wave, 240 V; 975 µF) in 400 µl OptiMEM medium.

Article Title: New histone supply regulates replication fork speed and PCNA unloading
Article Snippet: .. Expression plasmids were introduced by transfection with Lipofectamine (Invitrogen) or electroporation using a GenePulser Xcell (Bio-Rad Laboratories). ..

Article Title: Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4
Article Snippet: .. The schematic diagram of p-VBIG is shown in A. Aurantiochytrium sp. SK4 was transformed with p-VBIG by electroporation using a GenePulser Xcell™ (Bio-Rad, Hercules, CA, USA). .. The parameters of electroporation were set as 2 kV, 50 μF, and 300 Ω. Electroporated cells were resuspended in 50 mL of culture medium and cultivated for 16 hours in an orbital shaker at 150 rpm and 25 °C in the dark.

Article Title: Functional sequestration of microRNA-122 from Hepatitis C Virus by circular RNA sponges
Article Snippet: .. Cells were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer's instructions, or by electroporation using GenePulser Xcell (Bio-Rad). .. For the latter, cells were trypsinized, pelleted by centrifugation at 1500 rpm, rinsed with PBS and reconstituted in Cytomix (120 mM KCl, 0.15 mM CaCl2 , 10 mM K2 HPO4 /KH2 PO4 (pH 7.6), 25 mM Hepes, 2 mM EGTA, 5 mM MgCl2 , pH is adjusted to 7.6 with KOH; supplemented by 2 mM ATP and 5 mM glutathione before use) to 107 cells/ml.

Article Title: Pseudomonas mRNA 2.0: Boosting Gene Expression Through Enhanced mRNA Stability and Translational Efficiency
Article Snippet: .. Assembled plasmids were transformed into P. taiwanensis VLB120 by electroporation using a GenePulser Xcell (BioRad, Hercules, CA, USA) (settings: 2 mm cuvette gap, 2.5 kV, 200 Ω, 25 μF). .. For DNA integration into the att Tn7 locus, the mini-Tn7 delivery vector backbone developed by Zobel et al. ( ) was used and deployed through mating procedures.

Incubation:

Article Title: Dendritic Cells Actively Limit Interleukin-10 Production Under Inflammatory Conditions via DC-SCRIPT and Dual-Specificity Phosphatase 4
Article Snippet: .. A total of 10 µg siRNA was transferred to a 4-mm cuvette (Bio-Rad), and 10 × 106 DCs were added in 200 µL OptiMEM and incubated for 3 min before being pulsed with an exponential decay pulse at 300 V, 150 μF, in a Genepulser Xcell (Bio-Rad), as previously described ( ). .. Immediately after electroporation, the cells were transferred to pre-heated (37°C) phenol red-free RPMI 1640 culture medium supplemented with 1% ultra-glutamine, 10% (v/v) FCS, IL-4 (300 U/mL), and GM-CSF (450 U/mL).

Article Title: Functional OCT4-specific CD4+ and CD8+ T cells in healthy controls and ovarian cancer patients
Article Snippet: .. Twenty micrograms of mRNA were transferred to a 4-mm cuvette (Bio-Rad, 65-2089), and 8–10 × 106 cells were added in 180 μL of OptiMEM and incubated for 3 min before being pulsed in a Genepulser Xcell (Bio-Rad) with an exponential decay pulse of 300 V and capacitance of 150 μF. .. After electroporation, DCs were allow to rest for at least 2 h at 37°C in phenol red-free X-VIVO 15 medium (Lonza, BE04-744Q) supplemented with 6% HS before further treatment.

Transfection:

Article Title: RIG-I and MDA-5 Detection of Viral RNA-dependent RNA Polymerase Activity Restricts Positive-Strand RNA Virus Replication
Article Snippet: .. RD cells (0.2×106 per 60-mm dish) were transfected with DNA (0.2 µg/ml) by electroporation in GenePulser Xcell (Bio-Rad) instrument (settings: Exponential Wave, 190 V; 975 microfarads [µF]) in 400 µl OptiMEM medium (Invitrogen). .. RNA (2 µg/ml) and DNA (10 µg/ml) into MEF cells (0.25×106 per 60-mm dish) were transfected by electroporation (Exponential Wave, 240 V; 975 µF) in 400 µl OptiMEM medium.

Article Title: New histone supply regulates replication fork speed and PCNA unloading
Article Snippet: .. Expression plasmids were introduced by transfection with Lipofectamine (Invitrogen) or electroporation using a GenePulser Xcell (Bio-Rad Laboratories). ..

Article Title: Functional sequestration of microRNA-122 from Hepatitis C Virus by circular RNA sponges
Article Snippet: .. Cells were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer's instructions, or by electroporation using GenePulser Xcell (Bio-Rad). .. For the latter, cells were trypsinized, pelleted by centrifugation at 1500 rpm, rinsed with PBS and reconstituted in Cytomix (120 mM KCl, 0.15 mM CaCl2 , 10 mM K2 HPO4 /KH2 PO4 (pH 7.6), 25 mM Hepes, 2 mM EGTA, 5 mM MgCl2 , pH is adjusted to 7.6 with KOH; supplemented by 2 mM ATP and 5 mM glutathione before use) to 107 cells/ml.

Transformation Assay:

Article Title: Consequences of Cas9 cleavage in the chromosome of Escherichia coli
Article Snippet: .. The pCas9, pCas9::LacZ1 and pCas9::LacZ2 plasmids (40 ng each) were transformed into electrocompetent cells using 1 mm gap cuvette and the GenePulser Xcell (Bio-Rad) set to 1800 V, 25 uF, 200 Ω. ..

Article Title: Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4
Article Snippet: .. The schematic diagram of p-VBIG is shown in A. Aurantiochytrium sp. SK4 was transformed with p-VBIG by electroporation using a GenePulser Xcell™ (Bio-Rad, Hercules, CA, USA). .. The parameters of electroporation were set as 2 kV, 50 μF, and 300 Ω. Electroporated cells were resuspended in 50 mL of culture medium and cultivated for 16 hours in an orbital shaker at 150 rpm and 25 °C in the dark.

Article Title: Pseudomonas mRNA 2.0: Boosting Gene Expression Through Enhanced mRNA Stability and Translational Efficiency
Article Snippet: .. Assembled plasmids were transformed into P. taiwanensis VLB120 by electroporation using a GenePulser Xcell (BioRad, Hercules, CA, USA) (settings: 2 mm cuvette gap, 2.5 kV, 200 Ω, 25 μF). .. For DNA integration into the att Tn7 locus, the mini-Tn7 delivery vector backbone developed by Zobel et al. ( ) was used and deployed through mating procedures.

Expressing:

Article Title: New histone supply regulates replication fork speed and PCNA unloading
Article Snippet: .. Expression plasmids were introduced by transfection with Lipofectamine (Invitrogen) or electroporation using a GenePulser Xcell (Bio-Rad Laboratories). ..

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  • 90
    Bio-Rad s aureus rn4220
    Analysis of cell wall hydrolysis. (A) In vitro susceptibility of cell walls. Crude cell walls of Mu50 or MuM were subjected to degradation by autolytic enzymes from S. aureus COL. Preparations of these samples are described in Materials and Methods. The experiments were reproduced several times, and data from a single representative experiment are shown. (B) Triton X-100-induced autolysis of Mu50 and MuM cells. Values indicate the average of two independent experiments. (C) Zymographic analysis of the autolysins in SDS cell extracts from Mu50 and MuM. Samples were analyzed in a 10% resolving gel containing heat-killed <t>RN4220</t> cells. Lytic activity was detected by incubation of the gel with gentle agitation at 37°C in renaturing buffer. Equivalent amounts of protein samples were loaded. (D) Protein profiles in culture supernatants. Extracellular proteins from Mu50 and MuM were prepared as described in Materials and Methods. The gel was stained with Coomassie blue to detect the total secreted protein profile, and the protein band of autolysin was identified by MALDI-TOF MS-MS.
    S Aureus Rn4220, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s aureus rn4220/product/Bio-Rad
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    s aureus rn4220 - by Bioz Stars, 2020-08
    90/100 stars
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    92
    Bio-Rad sirna transfection
    SMN and Gemin protein levels are interdependent. ( A ) siRNAs were transfected and cytoplasmic cell lysates were collected 60 h later for Western analysis. Columns denote <t>siRNA</t> <t>transfections</t> with mock (no siRNA), SMN, G2 (Gemin2), G3 (Gemin3), G4 (Gemin4),
    Sirna Transfection, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sirna transfection/product/Bio-Rad
    Average 92 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    sirna transfection - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    89
    Bio-Rad bio rad genepulser xcell
    SMN and Gemin protein levels are interdependent. ( A ) siRNAs were transfected and cytoplasmic cell lysates were collected 60 h later for Western analysis. Columns denote <t>siRNA</t> <t>transfections</t> with mock (no siRNA), SMN, G2 (Gemin2), G3 (Gemin3), G4 (Gemin4),
    Bio Rad Genepulser Xcell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio rad genepulser xcell/product/Bio-Rad
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    bio rad genepulser xcell - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of cell wall hydrolysis. (A) In vitro susceptibility of cell walls. Crude cell walls of Mu50 or MuM were subjected to degradation by autolytic enzymes from S. aureus COL. Preparations of these samples are described in Materials and Methods. The experiments were reproduced several times, and data from a single representative experiment are shown. (B) Triton X-100-induced autolysis of Mu50 and MuM cells. Values indicate the average of two independent experiments. (C) Zymographic analysis of the autolysins in SDS cell extracts from Mu50 and MuM. Samples were analyzed in a 10% resolving gel containing heat-killed RN4220 cells. Lytic activity was detected by incubation of the gel with gentle agitation at 37°C in renaturing buffer. Equivalent amounts of protein samples were loaded. (D) Protein profiles in culture supernatants. Extracellular proteins from Mu50 and MuM were prepared as described in Materials and Methods. The gel was stained with Coomassie blue to detect the total secreted protein profile, and the protein band of autolysin was identified by MALDI-TOF MS-MS.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: A PBP 2 Mutant Devoid of the Transpeptidase Domain Abolishes Spermine-?-Lactam Synergy in Staphylococcus aureus Mu50

    doi: 10.1128/AAC.05415-11

    Figure Lengend Snippet: Analysis of cell wall hydrolysis. (A) In vitro susceptibility of cell walls. Crude cell walls of Mu50 or MuM were subjected to degradation by autolytic enzymes from S. aureus COL. Preparations of these samples are described in Materials and Methods. The experiments were reproduced several times, and data from a single representative experiment are shown. (B) Triton X-100-induced autolysis of Mu50 and MuM cells. Values indicate the average of two independent experiments. (C) Zymographic analysis of the autolysins in SDS cell extracts from Mu50 and MuM. Samples were analyzed in a 10% resolving gel containing heat-killed RN4220 cells. Lytic activity was detected by incubation of the gel with gentle agitation at 37°C in renaturing buffer. Equivalent amounts of protein samples were loaded. (D) Protein profiles in culture supernatants. Extracellular proteins from Mu50 and MuM were prepared as described in Materials and Methods. The gel was stained with Coomassie blue to detect the total secreted protein profile, and the protein band of autolysin was identified by MALDI-TOF MS-MS.

    Article Snippet: The recombinant plasmid pYX9 was first cloned into and extracted from E. coli and then electroporated into S. aureus RN4220 (Bio-Rad GenePulser Xcell) with parameters as described previously ( ).

    Techniques: In Vitro, Activity Assay, Incubation, Staining, Mass Spectrometry

    SMN and Gemin protein levels are interdependent. ( A ) siRNAs were transfected and cytoplasmic cell lysates were collected 60 h later for Western analysis. Columns denote siRNA transfections with mock (no siRNA), SMN, G2 (Gemin2), G3 (Gemin3), G4 (Gemin4),

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gemin proteins are required for efficient assembly of Sm-class ribonucleoproteins

    doi: 10.1073/pnas.0508947102

    Figure Lengend Snippet: SMN and Gemin protein levels are interdependent. ( A ) siRNAs were transfected and cytoplasmic cell lysates were collected 60 h later for Western analysis. Columns denote siRNA transfections with mock (no siRNA), SMN, G2 (Gemin2), G3 (Gemin3), G4 (Gemin4),

    Article Snippet: GFP-SmB DNA was electroporated 48 h after the initial siRNA transfection (Bio-Rad GenePulser XCell), and cell lysates were collected 24 h later.

    Techniques: Transfection, Western Blot

    Loss of SMN and Snurportin results in breakdown of nuclear CBs. ( A ) CB localization was detected by using anticoilin immunofluorescence ( Left ) in mock ( Upper ) or SMN siRNA ( Lower ) transfections. In Upper , note the prominent CBs, whereas in Lower , no CBs

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gemin proteins are required for efficient assembly of Sm-class ribonucleoproteins

    doi: 10.1073/pnas.0508947102

    Figure Lengend Snippet: Loss of SMN and Snurportin results in breakdown of nuclear CBs. ( A ) CB localization was detected by using anticoilin immunofluorescence ( Left ) in mock ( Upper ) or SMN siRNA ( Lower ) transfections. In Upper , note the prominent CBs, whereas in Lower , no CBs

    Article Snippet: GFP-SmB DNA was electroporated 48 h after the initial siRNA transfection (Bio-Rad GenePulser XCell), and cell lysates were collected 24 h later.

    Techniques: Immunofluorescence, Transfection

    SMN and Gemins2–4 are required for efficient Sm core assembly. ( A ) Cytoplasmic extracts were collected 60 h after mock, SMN, Gemin2, Gemin3, Gemin4, or Snurportin siRNA transfections. Extracts were incubated with wild-type U1 snRNA (+) or U1ΔSm

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Gemin proteins are required for efficient assembly of Sm-class ribonucleoproteins

    doi: 10.1073/pnas.0508947102

    Figure Lengend Snippet: SMN and Gemins2–4 are required for efficient Sm core assembly. ( A ) Cytoplasmic extracts were collected 60 h after mock, SMN, Gemin2, Gemin3, Gemin4, or Snurportin siRNA transfections. Extracts were incubated with wild-type U1 snRNA (+) or U1ΔSm

    Article Snippet: GFP-SmB DNA was electroporated 48 h after the initial siRNA transfection (Bio-Rad GenePulser XCell), and cell lysates were collected 24 h later.

    Techniques: Transfection, Incubation