Structured Review

Agilent technologies gene expression hybridization kit
Gene Expression Hybridization Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene expression hybridization kit/product/Agilent technologies
Average 97 stars, based on 597 article reviews
Price from $9.99 to $1999.99
gene expression hybridization kit - by Bioz Stars, 2020-05
97/100 stars

Images

Related Articles

Microarray:

Article Title: The Hippo Pathway Controls a Switch between Retinal Progenitor Cell Proliferation and Photoreceptor Cell Differentiation in Zebrafish
Article Snippet: .. RNA quality assurance, cDNA synthesis, and cRNA labeling and hybridization were carried out by Takara Bio Inc. (Otsu, Japan) using a Zebrafish (V3) Gene Expression Microarray 4X44K, the Low Input Quick Amp Labeling Kit, the Gene Expression Hybridization Kit, and the Gene Expression Wash Buffers Pack (all from Agilent Technologies) according to the manufacturer’s protocols. .. Raw data extraction and analyses were performed using Agilent Feature Extraction software (Agilent Technologies).

Article Title: How Many Parameters Does It Take to Describe Disease Tolerance?Tracking Resilience to Infections by Mapping Disease Space
Article Snippet: .. Samples were labeled using the Quick Amp Labeling Kit, One-Color (Agilent), and hybridized to 4x44K (V2) Drosophila Gene Expression Microarray (Agilent) using the Agilent Gene Expression Hybridization kit following the manufacturer’s protocol. .. Microarrays were scanned using an Agilent Technologies Scanner, and processed signal intensities were determined using Agilent’s Feature Extraction software.

Article Title: Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways
Article Snippet: .. Labelled cDNA was hybridized using the Agilent Gene Expression hybridization kit (part number 5188-5242) as described in the Agilent Two-Colour Microarray-Based Gene Expression Analysis v4.0 manual (publication number G4140-90050). .. Following hybridization, microarrays were washed in accordance with Agilent’s standard procedures and scanned using an Agilent DNA microarray scanner (model G2565A).

Labeling:

Article Title: The Hippo Pathway Controls a Switch between Retinal Progenitor Cell Proliferation and Photoreceptor Cell Differentiation in Zebrafish
Article Snippet: .. RNA quality assurance, cDNA synthesis, and cRNA labeling and hybridization were carried out by Takara Bio Inc. (Otsu, Japan) using a Zebrafish (V3) Gene Expression Microarray 4X44K, the Low Input Quick Amp Labeling Kit, the Gene Expression Hybridization Kit, and the Gene Expression Wash Buffers Pack (all from Agilent Technologies) according to the manufacturer’s protocols. .. Raw data extraction and analyses were performed using Agilent Feature Extraction software (Agilent Technologies).

Article Title: How Many Parameters Does It Take to Describe Disease Tolerance?Tracking Resilience to Infections by Mapping Disease Space
Article Snippet: .. Samples were labeled using the Quick Amp Labeling Kit, One-Color (Agilent), and hybridized to 4x44K (V2) Drosophila Gene Expression Microarray (Agilent) using the Agilent Gene Expression Hybridization kit following the manufacturer’s protocol. .. Microarrays were scanned using an Agilent Technologies Scanner, and processed signal intensities were determined using Agilent’s Feature Extraction software.

Article Title: Transcriptomic Profiles in Zebrafish Liver Permit the Discrimination of Surface Water with Pollution Gradient and Different Discharges
Article Snippet: .. Each slide was hybridized with 1.65 μg labeled RNA using Gene Expression Hybridization Kit (Agilent) in a hybridization oven (Agilent). ..

Expressing:

Article Title: The Hippo Pathway Controls a Switch between Retinal Progenitor Cell Proliferation and Photoreceptor Cell Differentiation in Zebrafish
Article Snippet: .. RNA quality assurance, cDNA synthesis, and cRNA labeling and hybridization were carried out by Takara Bio Inc. (Otsu, Japan) using a Zebrafish (V3) Gene Expression Microarray 4X44K, the Low Input Quick Amp Labeling Kit, the Gene Expression Hybridization Kit, and the Gene Expression Wash Buffers Pack (all from Agilent Technologies) according to the manufacturer’s protocols. .. Raw data extraction and analyses were performed using Agilent Feature Extraction software (Agilent Technologies).

Article Title: How Many Parameters Does It Take to Describe Disease Tolerance?Tracking Resilience to Infections by Mapping Disease Space
Article Snippet: .. Samples were labeled using the Quick Amp Labeling Kit, One-Color (Agilent), and hybridized to 4x44K (V2) Drosophila Gene Expression Microarray (Agilent) using the Agilent Gene Expression Hybridization kit following the manufacturer’s protocol. .. Microarrays were scanned using an Agilent Technologies Scanner, and processed signal intensities were determined using Agilent’s Feature Extraction software.

Article Title: Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location
Article Snippet: .. Hybridization reactions were performed at 65 °C for 17 h using Agilent’s Gene Expression Hybridization Kit. .. After washing, microarrays were scanned at 3 μm/pixel resolution using the Agilent DNA Microarray Scanner, and images were analyzed with Agilent Feature Extraction Software (Version 10.7.3.1, protocol GE1_107_Sep09).

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions. .. 1650 ng of cRNA was subjected to fragmentation (30 min at 60 °C) and then hybridized to 4x44K Human Whole-Genome 60-mer oligo-chips (G4112F, Agilent Technologies) in a rotary oven (10 rpm, 65C, 17 h).

Article Title: Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways
Article Snippet: .. Labelled cDNA was hybridized using the Agilent Gene Expression hybridization kit (part number 5188-5242) as described in the Agilent Two-Colour Microarray-Based Gene Expression Analysis v4.0 manual (publication number G4140-90050). .. Following hybridization, microarrays were washed in accordance with Agilent’s standard procedures and scanned using an Agilent DNA microarray scanner (model G2565A).

Article Title: Transcriptomic analysis of Campylobacter jejuni NCTC 11168 in response to epinephrine and norepinephrine
Article Snippet: .. Each slide was hybridized with 600 ng of Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US). .. Following 17 h of hybridization, the slides were washed in staining dishes with Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, CA, US).

Article Title: Transcriptomic Profiles in Zebrafish Liver Permit the Discrimination of Surface Water with Pollution Gradient and Different Discharges
Article Snippet: .. Each slide was hybridized with 1.65 μg labeled RNA using Gene Expression Hybridization Kit (Agilent) in a hybridization oven (Agilent). ..

Article Title: Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K-AKT signaling
Article Snippet: .. Each slide was hybridized with 1.65 µg cyanine 3-labeled cRNA using the Gene Expression Hybridization kit (cat. no. 5188–5242; Agilent Technologies, Inc.) in a hybridization oven (cat. no. G2545A; Agilent Technologies, Inc.) according to the manufacturer's protocol. .. After 17 h of hybridization, the slides were washed in staining dishes (cat. no. 121; Shandon™; Thermo Fisher Scientific, Inc.) with a Gene Expression Wash Buffer kit (cat. no. 5188–5327; Agilent Technologies, Inc.) according to the manufacturer's protocol ( , ).

Hybridization:

Article Title: The Hippo Pathway Controls a Switch between Retinal Progenitor Cell Proliferation and Photoreceptor Cell Differentiation in Zebrafish
Article Snippet: .. RNA quality assurance, cDNA synthesis, and cRNA labeling and hybridization were carried out by Takara Bio Inc. (Otsu, Japan) using a Zebrafish (V3) Gene Expression Microarray 4X44K, the Low Input Quick Amp Labeling Kit, the Gene Expression Hybridization Kit, and the Gene Expression Wash Buffers Pack (all from Agilent Technologies) according to the manufacturer’s protocols. .. Raw data extraction and analyses were performed using Agilent Feature Extraction software (Agilent Technologies).

Article Title: How Many Parameters Does It Take to Describe Disease Tolerance?Tracking Resilience to Infections by Mapping Disease Space
Article Snippet: .. Samples were labeled using the Quick Amp Labeling Kit, One-Color (Agilent), and hybridized to 4x44K (V2) Drosophila Gene Expression Microarray (Agilent) using the Agilent Gene Expression Hybridization kit following the manufacturer’s protocol. .. Microarrays were scanned using an Agilent Technologies Scanner, and processed signal intensities were determined using Agilent’s Feature Extraction software.

Article Title: Molecular alterations induced by a high-fat high-fiber diet in porcine adipose tissues: variations according to the anatomical fat location
Article Snippet: .. Hybridization reactions were performed at 65 °C for 17 h using Agilent’s Gene Expression Hybridization Kit. .. After washing, microarrays were scanned at 3 μm/pixel resolution using the Agilent DNA Microarray Scanner, and images were analyzed with Agilent Feature Extraction Software (Version 10.7.3.1, protocol GE1_107_Sep09).

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions. .. 1650 ng of cRNA was subjected to fragmentation (30 min at 60 °C) and then hybridized to 4x44K Human Whole-Genome 60-mer oligo-chips (G4112F, Agilent Technologies) in a rotary oven (10 rpm, 65C, 17 h).

Article Title: Bifidobacterium breve UCC2003 metabolises the human milk oligosaccharides lacto-N-tetraose and lacto-N-neo-tetraose through overlapping, yet distinct pathways
Article Snippet: .. Labelled cDNA was hybridized using the Agilent Gene Expression hybridization kit (part number 5188-5242) as described in the Agilent Two-Colour Microarray-Based Gene Expression Analysis v4.0 manual (publication number G4140-90050). .. Following hybridization, microarrays were washed in accordance with Agilent’s standard procedures and scanned using an Agilent DNA microarray scanner (model G2565A).

Article Title: Transcriptomic analysis of Campylobacter jejuni NCTC 11168 in response to epinephrine and norepinephrine
Article Snippet: .. Each slide was hybridized with 600 ng of Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US). .. Following 17 h of hybridization, the slides were washed in staining dishes with Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, CA, US).

Article Title: Transcriptomic Profiles in Zebrafish Liver Permit the Discrimination of Surface Water with Pollution Gradient and Different Discharges
Article Snippet: .. Each slide was hybridized with 1.65 μg labeled RNA using Gene Expression Hybridization Kit (Agilent) in a hybridization oven (Agilent). ..

Article Title: Investigation of gene expression profiles in a rat adjuvant arthritis model suggests an effective role of triptolide via PI3K-AKT signaling
Article Snippet: .. Each slide was hybridized with 1.65 µg cyanine 3-labeled cRNA using the Gene Expression Hybridization kit (cat. no. 5188–5242; Agilent Technologies, Inc.) in a hybridization oven (cat. no. G2545A; Agilent Technologies, Inc.) according to the manufacturer's protocol. .. After 17 h of hybridization, the slides were washed in staining dishes (cat. no. 121; Shandon™; Thermo Fisher Scientific, Inc.) with a Gene Expression Wash Buffer kit (cat. no. 5188–5327; Agilent Technologies, Inc.) according to the manufacturer's protocol ( , ).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 89
    Agilent technologies gene expression hybridisation kit
    In situ <t>hybridisation</t> of pluripotency-related genes reveals heterogeneous expression in cES and PGC cells in early embryos. A-H: In situ hybridisation for TRIM71 (A), KLF2 (B), NANOG (C), OCT4/POUV (D), SOX3 (E), ENS1/ERNI (F), LOC770611 (G), SOX7 (H), CFCB1 (I), OTX2 (J), EOMES (K), CDX2 (L), DAZL (M), DDX4 N), GTSF1 (O) was performed on pre-primitive-streak stage embryos (a), HH3 +/4 (primitive streak stage) embryos (b), cES (c), PGC (d) and 7 day old embryonic gonads (e) as indicated in Materials and Methods. Expression of NANOG (C), POUV/OCT4 (D), ENS1/ERNI (F) and CDX2 (L) is detected homogeneously in both cES and PGC cells. The other genes present a heterogeneous profile for cES and PGC cells.
    Gene Expression Hybridisation Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene expression hybridisation kit/product/Agilent technologies
    Average 89 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    gene expression hybridisation kit - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    97
    Agilent technologies gene expression hybridization kit
    In situ <t>hybridisation</t> of pluripotency-related genes reveals heterogeneous expression in cES and PGC cells in early embryos. A-H: In situ hybridisation for TRIM71 (A), KLF2 (B), NANOG (C), OCT4/POUV (D), SOX3 (E), ENS1/ERNI (F), LOC770611 (G), SOX7 (H), CFCB1 (I), OTX2 (J), EOMES (K), CDX2 (L), DAZL (M), DDX4 N), GTSF1 (O) was performed on pre-primitive-streak stage embryos (a), HH3 +/4 (primitive streak stage) embryos (b), cES (c), PGC (d) and 7 day old embryonic gonads (e) as indicated in Materials and Methods. Expression of NANOG (C), POUV/OCT4 (D), ENS1/ERNI (F) and CDX2 (L) is detected homogeneously in both cES and PGC cells. The other genes present a heterogeneous profile for cES and PGC cells.
    Gene Expression Hybridization Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene expression hybridization kit/product/Agilent technologies
    Average 97 stars, based on 597 article reviews
    Price from $9.99 to $1999.99
    gene expression hybridization kit - by Bioz Stars, 2020-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    In situ hybridisation of pluripotency-related genes reveals heterogeneous expression in cES and PGC cells in early embryos. A-H: In situ hybridisation for TRIM71 (A), KLF2 (B), NANOG (C), OCT4/POUV (D), SOX3 (E), ENS1/ERNI (F), LOC770611 (G), SOX7 (H), CFCB1 (I), OTX2 (J), EOMES (K), CDX2 (L), DAZL (M), DDX4 N), GTSF1 (O) was performed on pre-primitive-streak stage embryos (a), HH3 +/4 (primitive streak stage) embryos (b), cES (c), PGC (d) and 7 day old embryonic gonads (e) as indicated in Materials and Methods. Expression of NANOG (C), POUV/OCT4 (D), ENS1/ERNI (F) and CDX2 (L) is detected homogeneously in both cES and PGC cells. The other genes present a heterogeneous profile for cES and PGC cells.

    Journal: Stem Cell Research

    Article Title: Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC

    doi: 10.1016/j.scr.2014.11.005

    Figure Lengend Snippet: In situ hybridisation of pluripotency-related genes reveals heterogeneous expression in cES and PGC cells in early embryos. A-H: In situ hybridisation for TRIM71 (A), KLF2 (B), NANOG (C), OCT4/POUV (D), SOX3 (E), ENS1/ERNI (F), LOC770611 (G), SOX7 (H), CFCB1 (I), OTX2 (J), EOMES (K), CDX2 (L), DAZL (M), DDX4 N), GTSF1 (O) was performed on pre-primitive-streak stage embryos (a), HH3 +/4 (primitive streak stage) embryos (b), cES (c), PGC (d) and 7 day old embryonic gonads (e) as indicated in Materials and Methods. Expression of NANOG (C), POUV/OCT4 (D), ENS1/ERNI (F) and CDX2 (L) is detected homogeneously in both cES and PGC cells. The other genes present a heterogeneous profile for cES and PGC cells.

    Article Snippet: 1.65 μg of Cy3 labelled cRNA was fragmented for 30 minutes using the Gene Expression Hybridisation Kit (Agilent) and loaded on 4× 44k GE chicken V2 slides (Agilent).

    Techniques: In Situ, Hybridization, Expressing, Pyrolysis Gas Chromatography

    Validation of microarray results. A) RNA gel blot analysis of selected genes. Phosphorimage analysis was used to quantify the intensity of hybridisation. The expression ratios between T1, T2 and wild-type plants are shown below the lanes as log2 values. Ethidium bromide-stained rRNA bands are shown as loading controls. WT, wild-type; T1, T2, two independent TPS1 -transgenic lines. B) Correlation between microarray and northern results in T2/wild-type comparison. A statistically significant correlation (r = 0.9369, p = 2.376e −06 ) was obtained for all genes tested.

    Journal: PLoS ONE

    Article Title: Transcriptome Analysis of Potato Leaves Expressing the Trehalose-6-Phosphate Synthase 1 Gene of Yeast

    doi: 10.1371/journal.pone.0023466

    Figure Lengend Snippet: Validation of microarray results. A) RNA gel blot analysis of selected genes. Phosphorimage analysis was used to quantify the intensity of hybridisation. The expression ratios between T1, T2 and wild-type plants are shown below the lanes as log2 values. Ethidium bromide-stained rRNA bands are shown as loading controls. WT, wild-type; T1, T2, two independent TPS1 -transgenic lines. B) Correlation between microarray and northern results in T2/wild-type comparison. A statistically significant correlation (r = 0.9369, p = 2.376e −06 ) was obtained for all genes tested.

    Article Snippet: The hybridisation mixture was set up using a Gene Expression Hybridisation Kit (Agilent) and applied to the microarray, which was then hybridised at 65°C for 17 h, followed by two subsequent washes in the appropriate Agilent wash solutions for one minute each at RT and 37°C, respectively.

    Techniques: Microarray, Western Blot, Hybridization, Expressing, Staining, Transgenic Assay, Northern Blot