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Agilent technologies gene expression hybridization kit
Gene Expression Hybridization Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene expression hybridization kit/product/Agilent technologies
Average 99 stars, based on 482 article reviews
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gene expression hybridization kit - by Bioz Stars, 2019-10
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Related Articles

Transfection:

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: After 48 h transfection with E7 siRNA #2, total RNA of Caski was extracted using TRIZOL Reagent (Life technologies, Carlsbad, USA) following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, USA). .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: RNA from 293T cells transfected with HA-DOCK10.1 or empty plasmid were used as tests, and untransfected 293T cells as reference. .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit.

Amplification:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Qualified total RNA was purified using RNeasy mini kit (QIAGEN) and RNase-free DNase Set (QIAGEN), and was amplified and labeled using Low Input Quick Amp Labling Kit (Agilent technologies) according to the manufacturer's instructions. .. Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: Two hundred nanograms of total RNA was amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol. .. A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: The enriched circRNAs were amplified and transcribed into fluorescent complementary RNA (cRNA) by Low Input Quick Amp WT Labeling Kit (Agilent technologies, Santa Clara, USA), following the manufacturer's instructions. .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Total RNA was amplified and labeled using a Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions. .. Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Total RNA was amplified and labeled with the Low Input Quick Amp Labeling Kit, One-Color (Agilent technologies, Santa Clara, CA, US). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven.

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: One-hundred nanograms of total RNA were reverse-transcribed into double-strand cDNAs by AffinityScript multiple-temperature reverse-transcriptase and amplified for 2 h at 40°C. .. Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions.

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies).

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: Amplification and labeling were performed by T7-polymerase in vitro transcription to produce Cy3-labeled cRNA. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions.

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Total RNA was amplified and labeled by Low Input Quick Amp Labeling kit, One-Color (cat. no. 5190-2305; Agilent Technologies, Inc.). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.).

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: Using random nucleotide-based T7 promoter primers included in the Low Input Quick Amp WT labelling kit (Agilent Technologies, USA), 25 ng of RNA was amplified and labelled. .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Article Title: Potential biological functions of microvesicles derived from adenoid cystic carcinoma
Article Snippet: In brief, the amplification and labeling of 500 ng of total RNA was performed according to the Agilent Low Input Quick Amp Labeling kit's protocol using Cy3 (Agilent Technologies, Inc.). .. Each slide was hybridized with 1.65 µg Cy3-labeled cRNA using a Gene Expression Hybridization kit (Agilent Technologies, Inc.) for 17 h at 50°C, according to the manufacturer's protocol.

In Vitro:

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: The resulting cDNAs were subsequently used for in vitro transcription by the T7 RNA polymerase and labeled with cyanine-3-labeled cytosine triphosphate (Perkin Elmer, Waltham, MA) for 2 h at 40°C using a Low Input Quick-Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol. .. Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: Amplification and labeling were performed by T7-polymerase in vitro transcription to produce Cy3-labeled cRNA. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions.

Synthesized:

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: Microarrays were synthesized using the proprietary non-contact SurePrint Ink-jet technology (Agilent Technologies, USA). .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Software:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions. .. Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit. .. A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions. .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions. .. Slides were scanned using an Agilent Microarray Scanner (Agilent Technologies, Santa Clara, CA, US) with the default settings.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Data were extracted by feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US), and normalized by Quantile algorithm, Gene Spring Software 11.0 (Agilent technologies, Santa Clara, CA, US). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven.

Article Title: Evaluation of Current and New Biomarkers in Severe Preeclampsia: A Microarray Approach Reveals the VSIG4 Gene as a Potential Blood Biomarker
Article Snippet: The hybridization was performed for 17 hours at 65°C using the gene expression hybridization kit (Agilent Technologies, Massy, France). .. The microarray slides were scanned with a G2505C DNA Microarray scanner at a resolution of 5 µm.

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions. .. After washing in GE washing buffer, each slide was scanned with an Agilent Microarray Scanner G2505C.

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies). .. Slides were scanned using an Agilent Microarray Scanner (Cat.# G2565CA) and data extracted with Feature Extraction software 10.7.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit. .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions.

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.). .. Slides were scanned by Agilent Microarray Scanner (cat. no. G2565CA; Agilent Technologies, Inc.) with default settings.

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed. .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Article Title: Potential biological functions of microvesicles derived from adenoid cystic carcinoma
Article Snippet: Each slide was hybridized with 1.65 µg Cy3-labeled cRNA using a Gene Expression Hybridization kit (Agilent Technologies, Inc.) for 17 h at 50°C, according to the manufacturer's protocol. .. Following hybridization and washing with buffer (0.1X saline sodium citrate, 0.5% SDS), the processed slides were scanned with the Agilent Microarray Scanner (Agilent Technologies, Inc.).

Isolation:

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: Paragraph title: RNA isolation and microarray analysis ... The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit.

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Total RNA of the renal cortex of the mice was extracted and purified using mirVana™ miRNA Isolation kit (cat. no. AM1561; Ambion; Thermo Fisher Scientific, Inc.) and checked for a RNA integrity Number (RIN) to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.).

Labeling:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Qualified total RNA was purified using RNeasy mini kit (QIAGEN) and RNase-free DNase Set (QIAGEN), and was amplified and labeled using Low Input Quick Amp Labling Kit (Agilent technologies) according to the manufacturer's instructions. .. Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: Two hundred nanograms of total RNA was amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol. .. A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit.

Article Title: Gene expression associated with suicide attempts in US veterans
Article Snippet: Agilent Low Input Quick Amp Labeling Kit, along with Agilent Two-Color Spike-Mix (Agilent Technologies) was used to label experimental (Cy5-labeled) and reference samples—Stratagene Human universal reference RNA (Cy3-labeled, Agilent Technologies). .. All labeled sample reactions were then subjected to fragmentation followed by 17 h hybridization using the Agilent Gene Expression Hybridization Kit. .. Following scanning and extraction, individual mRNA microarrays were LOESS-normalized and the bottom 30% of the distribution of probes were filtered out as were those that did not have a corresponding RefSeq gene annotation.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: Labeled cRNA were purified by RNeasy mini kit (Qiagen, Hamburg, Germany). .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Labeled cRNA was purified using an RNeasy Mini Kit (QIAGEN, GmBH, Germany). .. Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Labeled cRNA was purified by RNeasy mini kit (QIAGEN, GmBH, Germany). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven.

Article Title: Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma, et al. Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
Article Snippet: In brief, cDNA synthesis and cRNA labeling with the cyanine 3 dye were carried out using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies). .. The cyanine 3‐labeled cRNA was purified, fragmented, and hybridized on a Human Gene Expression 4 × 44K v2 Microarray Chip containing 27 958 Entrez Gene RNAs, using a Gene Expression Hybridization kit (Agilent Technologies).

Article Title: Evaluation of Current and New Biomarkers in Severe Preeclampsia: A Microarray Approach Reveals the VSIG4 Gene as a Potential Blood Biomarker
Article Snippet: In brief, 400 ng of RNA was labeled with cyanine-3 CTP using the quick amp labeling kit (Agilent Technologies, Massy, France). .. The hybridization was performed for 17 hours at 65°C using the gene expression hybridization kit (Agilent Technologies, Massy, France).

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: Differentially labeled samples of 1650 ng of cRNA were hybridized on Agilent 4 x 44K whole genome arrays ver. .. Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions.

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies). .. Slides were scanned using an Agilent Microarray Scanner (Cat.# G2565CA) and data extracted with Feature Extraction software 10.7.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: These microarray experiments were designated as KO (N = 10) or WT (N = 10), and were performed using Agilent Mouse GE 4 × 44K v2 Microarrays targeting 39,430 Entrez Gene RNAs. .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit. .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: Amplification and labeling were performed by T7-polymerase in vitro transcription to produce Cy3-labeled cRNA. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions.

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Labeled cRNA were purified using an RNeasy mini kit (cat. no. 74106; Qiagen, GmBH, Germany). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.).

Article Title: Potential biological functions of microvesicles derived from adenoid cystic carcinoma
Article Snippet: In brief, the amplification and labeling of 500 ng of total RNA was performed according to the Agilent Low Input Quick Amp Labeling kit's protocol using Cy3 (Agilent Technologies, Inc.). .. Each slide was hybridized with 1.65 µg Cy3-labeled cRNA using a Gene Expression Hybridization kit (Agilent Technologies, Inc.) for 17 h at 50°C, according to the manufacturer's protocol.

Article Title: Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer
Article Snippet: Labeled cRNA was then purified using RNeasy® mini spin columns (Qiagen). .. Hybridization was performed using the Agilent Gene Expression Hybridization Kit; samples were hybridized by rotating at 10 rpm (65°C) for 17 h and then washed before scanning.

Mouse Assay:

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: The cochleae (n = 8 per mouse) of wild-type, DT-treated wild-type, Prestin -hDTR , and DT-treated Prestin -hDTR mice at P35 (Fig. ) were used for microarray analysis. .. A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit.

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: CD8+ T cells from the livers of 13–14 week old female TG and TGC3 mice were first enriched by positive selection with anti-CD8 magnetic microbeads (Miltenyi Biotech) and then purified by FACS Aria (BD Bioscience). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies).

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: RNA samples from SBC isolated from Dock10-KO mice and WT mice were used as tests, and a pooled sample composed of equimolar amounts of RNA from 5 KO mice was used as reference . .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit.

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Total RNA of the renal cortex of the mice was extracted and purified using mirVana™ miRNA Isolation kit (cat. no. AM1561; Ambion; Thermo Fisher Scientific, Inc.) and checked for a RNA integrity Number (RIN) to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.).

Microarray:

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: Paragraph title: Microarray analysis ... A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit.

Article Title: Gene expression associated with suicide attempts in US veterans
Article Snippet: Whole-Human Genome Microarray 60 mer Oligo slides printed with Agilent SurePrint Technology were obtained from Agilent Technologies (Agilent Technologies). .. All labeled sample reactions were then subjected to fragmentation followed by 17 h hybridization using the Agilent Gene Expression Hybridization Kit.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: Paragraph title: CircRNA microarray analysis ... In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions. .. After 17 h of hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) using the Gene Expression Wash Buffer Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Paragraph title: MiRNA and lncRNA microarray analysis ... Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven.

Article Title: Adjuvanted influenza-H1N1 vaccination reveals lymphoid signatures of age-dependent early responses and of clinical adverse events
Article Snippet: The samples were chosen from patients representative of our overall cohort, and include both vaccine responders (R, 34/46) and non-responders (NR, 12/46; 6/46 “baseline” non-responders, with no detectable pre-existing H1N1 antibody response and no increase in post-vaccine antibody titers by either HAI or MN), and low (32/46) and medium/high (14/46) AE subjects. .. RNAs extracted from PBMCs were processed in two batches according to the Agilent protocol and with Agilent Gene Expression Hybridization Kit, then hybridized to Agilent 4x44K v2 Human Whole Genome microarrays, and scanned using Agilent’s Microarray Scanner System (Agilent Technologies). .. After QC, 173 arrays were retained in the analysis and the batch effect was removed with the method ComBat from the R package sva .

Article Title: Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma, et al. Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
Article Snippet: In brief, cDNA synthesis and cRNA labeling with the cyanine 3 dye were carried out using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies). .. The cyanine 3‐labeled cRNA was purified, fragmented, and hybridized on a Human Gene Expression 4 × 44K v2 Microarray Chip containing 27 958 Entrez Gene RNAs, using a Gene Expression Hybridization kit (Agilent Technologies). .. The raw and normalized microarray data have been submitted to the GEO database at NCBI (accession no. GSE116000 ; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE116000 ).

Article Title: Evaluation of Current and New Biomarkers in Severe Preeclampsia: A Microarray Approach Reveals the VSIG4 Gene as a Potential Blood Biomarker
Article Snippet: The microarray study was performed using microarray chips that included 45,000 probes (1 microarray for each patient, 4X44K Whole Human Genome microarray G4112F) and the One Color Microarray Based Gene Expression Analysis based on the Agilent Technologies procedures recently described [ ]. .. The hybridization was performed for 17 hours at 65°C using the gene expression hybridization kit (Agilent Technologies, Massy, France).

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: Paragraph title: Microarray experiment ... Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: These microarray experiments were designated as KO (N = 10) or WT (N = 10), and were performed using Agilent Mouse GE 4 × 44K v2 Microarrays targeting 39,430 Entrez Gene RNAs. .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit. .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions.

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Paragraph title: Microarray analysis ... Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.).

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: Paragraph title: Microarray processing and data analysis ... For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Article Title: Overexpression of long non-coding RNA RP11-396F22.1 correlates poor prognosis of patients with early-stage cervical cancer
Article Snippet: Human cervical cancer cells were transfected with either 50 nmol si-RP11-396F22.1 or si-NC using HiPerFect Transfection (cat NO. 301705) according to the manufacturer’s instruction (QIAGEN, Germany). .. Molecular microarray was used in this procedure according to the protocol of Agilent Gene Expression Hybridization Kit. .. 10× Blocking Agent was made up to a volume of 500 μl with nuclease-free water, then votex the agent gently.

Article Title: Potential biological functions of microvesicles derived from adenoid cystic carcinoma
Article Snippet: Paragraph title: Microarray analysis ... Each slide was hybridized with 1.65 µg Cy3-labeled cRNA using a Gene Expression Hybridization kit (Agilent Technologies, Inc.) for 17 h at 50°C, according to the manufacturer's protocol.

Article Title: Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer
Article Snippet: Paragraph title: Gene expression microarray ... Hybridization was performed using the Agilent Gene Expression Hybridization Kit; samples were hybridized by rotating at 10 rpm (65°C) for 17 h and then washed before scanning.

Purification:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Qualified total RNA was purified using RNeasy mini kit (QIAGEN) and RNase-free DNase Set (QIAGEN), and was amplified and labeled using Low Input Quick Amp Labling Kit (Agilent technologies) according to the manufacturer's instructions. .. Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: Labeled cRNA were purified by RNeasy mini kit (Qiagen, Hamburg, Germany). .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Labeled cRNA was purified using an RNeasy Mini Kit (QIAGEN, GmBH, Germany). .. Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Labeled cRNA was purified by RNeasy mini kit (QIAGEN, GmBH, Germany). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven.

Article Title: Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma, et al. Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
Article Snippet: In brief, cDNA synthesis and cRNA labeling with the cyanine 3 dye were carried out using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies). .. The cyanine 3‐labeled cRNA was purified, fragmented, and hybridized on a Human Gene Expression 4 × 44K v2 Microarray Chip containing 27 958 Entrez Gene RNAs, using a Gene Expression Hybridization kit (Agilent Technologies). .. The raw and normalized microarray data have been submitted to the GEO database at NCBI (accession no. GSE116000 ; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE116000 ).

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Total RNA was extracted and purified using an miRNeasy Mini Kit (Cat.#217004, QIAGEN, GmBH, Germany) and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies).

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Labeled cRNA were purified using an RNeasy mini kit (cat. no. 74106; Qiagen, GmBH, Germany). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.).

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: The labelled cRNA were purified using RNeasy columns (QIAGEN Inc, USA). cRNA yields and specific activities were measured using Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Article Title: Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer
Article Snippet: Labeled cRNA was then purified using RNeasy® mini spin columns (Qiagen). .. Hybridization was performed using the Agilent Gene Expression Hybridization Kit; samples were hybridized by rotating at 10 rpm (65°C) for 17 h and then washed before scanning.

Selection:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions. .. Raw data were normalized using quantile algorithm available in GeneSpring Software 12.6.1 (Agilent technologies).

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: CD8+ T cells from the livers of 13–14 week old female TG and TGC3 mice were first enriched by positive selection with anti-CD8 magnetic microbeads (Miltenyi Biotech) and then purified by FACS Aria (BD Bioscience). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies).

Spectrophotometry:

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: After labeling, the rates of dye incorporation and quantification were measured with a Nanodrop ND-1000 v3.5.2 spectrophotometer (Thermo Scientific) and then were fragmented for 30 min at 60°C in the dark. .. Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: The dye incorporation rate was assessed with a Nanodrop ND-1000 spectrophotometer and was consistently > 9 pmolCy3/μgRNA. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions.

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: The labelled cRNA were purified using RNeasy columns (QIAGEN Inc, USA). cRNA yields and specific activities were measured using Nanodrop 2000c spectrophotometer (Thermo Scientific, USA). .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Expressing:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Qualified total RNA was purified using RNeasy mini kit (QIAGEN) and RNase-free DNase Set (QIAGEN), and was amplified and labeled using Low Input Quick Amp Labling Kit (Agilent technologies) according to the manufacturer's instructions. .. Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions. .. After hybridization, the slides were washed with Gene Expression Wash Buffer Kit (Agilent Technologies) in staining dishes (Thermo, Shandong) according to the manufacturer's instructions.

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: Two hundred nanograms of total RNA was amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol. .. A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit. .. Hybridizations were performed for 17 hours at 65 °C and 10 rpm in a rotating hybridization oven.

Article Title: Gene expression associated with suicide attempts in US veterans
Article Snippet: Agilent Low Input Quick Amp Labeling Kit, along with Agilent Two-Color Spike-Mix (Agilent Technologies) was used to label experimental (Cy5-labeled) and reference samples—Stratagene Human universal reference RNA (Cy3-labeled, Agilent Technologies). .. All labeled sample reactions were then subjected to fragmentation followed by 17 h hybridization using the Agilent Gene Expression Hybridization Kit. .. Following scanning and extraction, individual mRNA microarrays were LOESS-normalized and the bottom 30% of the distribution of probes were filtered out as were those that did not have a corresponding RefSeq gene annotation.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: Labeled cRNA were purified by RNeasy mini kit (Qiagen, Hamburg, Germany). .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions. .. After 17 h hybridization, slides were washed in staining dishes with Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, USA) followed the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Labeled cRNA was purified using an RNeasy Mini Kit (QIAGEN, GmBH, Germany). .. Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions. .. After 17 h of hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) using the Gene Expression Wash Buffer Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Labeled cRNA was purified by RNeasy mini kit (QIAGEN, GmBH, Germany). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven. .. After 17 hours hybridization, slides were washed in staining dishes with the Gene Expression Wash Buffer Kit.

Article Title: Adjuvanted influenza-H1N1 vaccination reveals lymphoid signatures of age-dependent early responses and of clinical adverse events
Article Snippet: The samples were chosen from patients representative of our overall cohort, and include both vaccine responders (R, 34/46) and non-responders (NR, 12/46; 6/46 “baseline” non-responders, with no detectable pre-existing H1N1 antibody response and no increase in post-vaccine antibody titers by either HAI or MN), and low (32/46) and medium/high (14/46) AE subjects. .. RNAs extracted from PBMCs were processed in two batches according to the Agilent protocol and with Agilent Gene Expression Hybridization Kit, then hybridized to Agilent 4x44K v2 Human Whole Genome microarrays, and scanned using Agilent’s Microarray Scanner System (Agilent Technologies). .. After QC, 173 arrays were retained in the analysis and the batch effect was removed with the method ComBat from the R package sva .

Article Title: Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma, et al. Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
Article Snippet: In brief, cDNA synthesis and cRNA labeling with the cyanine 3 dye were carried out using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies). .. The cyanine 3‐labeled cRNA was purified, fragmented, and hybridized on a Human Gene Expression 4 × 44K v2 Microarray Chip containing 27 958 Entrez Gene RNAs, using a Gene Expression Hybridization kit (Agilent Technologies). .. The raw and normalized microarray data have been submitted to the GEO database at NCBI (accession no. GSE116000 ; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE116000 ).

Article Title: Evaluation of Current and New Biomarkers in Severe Preeclampsia: A Microarray Approach Reveals the VSIG4 Gene as a Potential Blood Biomarker
Article Snippet: In brief, 400 ng of RNA was labeled with cyanine-3 CTP using the quick amp labeling kit (Agilent Technologies, Massy, France). .. The hybridization was performed for 17 hours at 65°C using the gene expression hybridization kit (Agilent Technologies, Massy, France). .. The microarray slides were scanned with a G2505C DNA Microarray scanner at a resolution of 5 µm.

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: 2.0 (Agilent Design #026652) at 65°C for 17 h with rotation in the dark. .. Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions. .. After washing in GE washing buffer, each slide was scanned with an Agilent Microarray Scanner G2505C.

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies). .. Slides were scanned using an Agilent Microarray Scanner (Cat.# G2565CA) and data extracted with Feature Extraction software 10.7.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: These microarray experiments were designated as KO (N = 10) or WT (N = 10), and were performed using Agilent Mouse GE 4 × 44K v2 Microarrays targeting 39,430 Entrez Gene RNAs. .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit. .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: The dye incorporation rate was assessed with a Nanodrop ND-1000 spectrophotometer and was consistently > 9 pmolCy3/μgRNA. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions. .. 1650 ng of cRNA was subjected to fragmentation (30 min at 60 °C) and then hybridized to 4x44K Human Whole-Genome 60-mer oligo-chips (G4112F, Agilent Technologies) in a rotary oven (10 rpm, 65C, 17 h).

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Labeled cRNA were purified using an RNeasy mini kit (cat. no. 74106; Qiagen, GmBH, Germany). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.). .. Following 17 h hybridization, slides were washed in staining dishes (cat. no. 121; Thermo Fisher Scientific, Inc.) with Gene Expression Wash Buffer kit (cat. no. 5188-5327, Agilent Technologies, Inc.).

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: The microarray specifications and design have been described earlier . .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed. .. Hybridization and slide processing were done according to the procedure described by Agilent Technologies.

Article Title: Overexpression of long non-coding RNA RP11-396F22.1 correlates poor prognosis of patients with early-stage cervical cancer
Article Snippet: Human cervical cancer cells were transfected with either 50 nmol si-RP11-396F22.1 or si-NC using HiPerFect Transfection (cat NO. 301705) according to the manufacturer’s instruction (QIAGEN, Germany). .. Molecular microarray was used in this procedure according to the protocol of Agilent Gene Expression Hybridization Kit. .. 10× Blocking Agent was made up to a volume of 500 μl with nuclease-free water, then votex the agent gently.

Article Title: Potential biological functions of microvesicles derived from adenoid cystic carcinoma
Article Snippet: In brief, the amplification and labeling of 500 ng of total RNA was performed according to the Agilent Low Input Quick Amp Labeling kit's protocol using Cy3 (Agilent Technologies, Inc.). .. Each slide was hybridized with 1.65 µg Cy3-labeled cRNA using a Gene Expression Hybridization kit (Agilent Technologies, Inc.) for 17 h at 50°C, according to the manufacturer's protocol. .. Following hybridization and washing with buffer (0.1X saline sodium citrate, 0.5% SDS), the processed slides were scanned with the Agilent Microarray Scanner (Agilent Technologies, Inc.).

Article Title: Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer
Article Snippet: Labeled cRNA was then purified using RNeasy® mini spin columns (Qiagen). .. Hybridization was performed using the Agilent Gene Expression Hybridization Kit; samples were hybridized by rotating at 10 rpm (65°C) for 17 h and then washed before scanning. .. Gene expression microarray slides were scanned with the microarray scanner and analyzed with FEATURE EXTRACTION v10.1.1.1.

Staining:

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: After hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) with the Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, CA, US). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven.

FACS:

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: The CD8+ T cell sorting purity was greater than 95%. .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies).

Chloramphenicol Acetyltransferase Assay:

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Total RNA was extracted and purified using an miRNeasy Mini Kit (Cat.#217004, QIAGEN, GmBH, Germany) and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies).

T-Test:

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed. .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed.

Chromatin Immunoprecipitation:

Article Title: Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma, et al. Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
Article Snippet: In brief, cDNA synthesis and cRNA labeling with the cyanine 3 dye were carried out using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies). .. The cyanine 3‐labeled cRNA was purified, fragmented, and hybridized on a Human Gene Expression 4 × 44K v2 Microarray Chip containing 27 958 Entrez Gene RNAs, using a Gene Expression Hybridization kit (Agilent Technologies). .. The raw and normalized microarray data have been submitted to the GEO database at NCBI (accession no. GSE116000 ; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE116000 ).

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies). .. Raw data were normalized using the Quantile algorithm, Gene Spring Software 12.6.1.

Plasmid Preparation:

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: RNA from 293T cells transfected with HA-DOCK10.1 or empty plasmid were used as tests, and untransfected 293T cells as reference. .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit.

Hybridization:

Article Title: MICAL2 Mediates p53 Ubiquitin Degradation through Oxidating p53 Methionine 40 and 160 and Promotes Colorectal Cancer Malignance
Article Snippet: Qualified total RNA was purified using RNeasy mini kit (QIAGEN) and RNase-free DNase Set (QIAGEN), and was amplified and labeled using Low Input Quick Amp Labling Kit (Agilent technologies) according to the manufacturer's instructions. .. Each slide was hybridized with Cy3-labled cRNA using Gene Expression Hybridization Kit (Agilent Technologies) according to the manufacturer's instructions. .. After hybridization, the slides were washed with Gene Expression Wash Buffer Kit (Agilent Technologies) in staining dishes (Thermo, Shandong) according to the manufacturer's instructions.

Article Title: OHC-TRECK: A Novel System Using a Mouse Model for Investigation of the Molecular Mechanisms Associated with Outer Hair Cell Death in the Inner Ear
Article Snippet: Two hundred nanograms of total RNA was amplified and labeled using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol. .. A total of 1.65 µg of Cy3-labeled cRNA was fragmented using the protocol for the Agilent Gene Expression Hybridization Kit. .. Hybridizations were performed for 17 hours at 65 °C and 10 rpm in a rotating hybridization oven.

Article Title: Gene expression associated with suicide attempts in US veterans
Article Snippet: Agilent Low Input Quick Amp Labeling Kit, along with Agilent Two-Color Spike-Mix (Agilent Technologies) was used to label experimental (Cy5-labeled) and reference samples—Stratagene Human universal reference RNA (Cy3-labeled, Agilent Technologies). .. All labeled sample reactions were then subjected to fragmentation followed by 17 h hybridization using the Agilent Gene Expression Hybridization Kit. .. Following scanning and extraction, individual mRNA microarrays were LOESS-normalized and the bottom 30% of the distribution of probes were filtered out as were those that did not have a corresponding RefSeq gene annotation.

Article Title: Human papillomavirus 16 E7 oncoprotein alters the expression profiles of circular RNAs in Caski cells
Article Snippet: Labeled cRNA were purified by RNeasy mini kit (Qiagen, Hamburg, Germany). .. In the experiment, each slide was hybridized with 1.65 μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, USA), according to the manufacturer's instructions. .. After 17 h hybridization, slides were washed in staining dishes with Gene Expression Wash Buffer Kit (Agilent technologies, Santa Clara, USA) followed the manufacturer's instructions.

Article Title: TMED3 promotes hepatocellular carcinoma progression via IL-11/STAT3 signaling
Article Snippet: Labeled cRNA was purified using an RNeasy Mini Kit (QIAGEN, GmBH, Germany). .. Each slide was hybridized with 1.65 μg of Cy3-labeled cRNA in a hybridization oven using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions. .. After 17 h of hybridization, slides were washed in staining dishes (Thermo Shandon, Waltham, MA, US) using the Gene Expression Wash Buffer Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions.

Article Title: Biological characteristics of a new human glioma cell line transformed into A2B5+ stem cells
Article Snippet: Labeled cRNA was purified by RNeasy mini kit (QIAGEN, GmBH, Germany). .. Each Slide was hybridized with 1.65 μg Cy3-labeled cRNA using the Gene Expression Hybridization Kit (Agilent technologies, Santa Clara, CA, US) in Hybridization Oven. .. After 17 hours hybridization, slides were washed in staining dishes with the Gene Expression Wash Buffer Kit.

Article Title: Adjuvanted influenza-H1N1 vaccination reveals lymphoid signatures of age-dependent early responses and of clinical adverse events
Article Snippet: The samples were chosen from patients representative of our overall cohort, and include both vaccine responders (R, 34/46) and non-responders (NR, 12/46; 6/46 “baseline” non-responders, with no detectable pre-existing H1N1 antibody response and no increase in post-vaccine antibody titers by either HAI or MN), and low (32/46) and medium/high (14/46) AE subjects. .. RNAs extracted from PBMCs were processed in two batches according to the Agilent protocol and with Agilent Gene Expression Hybridization Kit, then hybridized to Agilent 4x44K v2 Human Whole Genome microarrays, and scanned using Agilent’s Microarray Scanner System (Agilent Technologies). .. After QC, 173 arrays were retained in the analysis and the batch effect was removed with the method ComBat from the R package sva .

Article Title: Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma, et al. Establishment and characterization of CRISPR/Cas9‐mediated NF2−/− human mesothelial cell line: Molecular insight into fibroblast growth factor receptor 2 in malignant pleural mesothelioma
Article Snippet: In brief, cDNA synthesis and cRNA labeling with the cyanine 3 dye were carried out using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies). .. The cyanine 3‐labeled cRNA was purified, fragmented, and hybridized on a Human Gene Expression 4 × 44K v2 Microarray Chip containing 27 958 Entrez Gene RNAs, using a Gene Expression Hybridization kit (Agilent Technologies). .. The raw and normalized microarray data have been submitted to the GEO database at NCBI (accession no. GSE116000 ; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE116000 ).

Article Title: Evaluation of Current and New Biomarkers in Severe Preeclampsia: A Microarray Approach Reveals the VSIG4 Gene as a Potential Blood Biomarker
Article Snippet: In brief, 400 ng of RNA was labeled with cyanine-3 CTP using the quick amp labeling kit (Agilent Technologies, Massy, France). .. The hybridization was performed for 17 hours at 65°C using the gene expression hybridization kit (Agilent Technologies, Massy, France). .. The microarray slides were scanned with a G2505C DNA Microarray scanner at a resolution of 5 µm.

Article Title: Characterization of miR-122-independent propagation of HCV
Article Snippet: 2.0 (Agilent Design #026652) at 65°C for 17 h with rotation in the dark. .. Hybridization was performed using a Gene Expression Hybridization Kit (Agilent Technologies) following the manufacturer’s instructions. .. After washing in GE washing buffer, each slide was scanned with an Agilent Microarray Scanner G2505C.

Article Title: Chemokine Receptor CXCR3 Deficiency Exacerbates Murine Autoimmune Cholangitis by Promoting Pathogenic CD8+ T Cell Activation
Article Snippet: Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat.# 5190-2305, Agilent technologies). .. Labeled cRNA, using an RNeasy mini kit (Cat.# 74106, QIAGEN), were hybridized with a Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies). .. Slides were scanned using an Agilent Microarray Scanner (Cat.# G2565CA) and data extracted with Feature Extraction software 10.7.

Article Title: The role of DOCK10 in the regulation of the transcriptome and aging
Article Snippet: These microarray experiments were designated as KO (N = 10) or WT (N = 10), and were performed using Agilent Mouse GE 4 × 44K v2 Microarrays targeting 39,430 Entrez Gene RNAs. .. The labeled cRNAs were mixed together and hybridized onto the microarray slides using the Agilent Gene Expression Hybridization kit. .. After hybridization, the microarray slides were washed and scanned in an Agilent G2565CA DNA Microarray Scanner.

Article Title: Wound healing and longevity: Lessons from long-lived αMUPA mice
Article Snippet: The dye incorporation rate was assessed with a Nanodrop ND-1000 spectrophotometer and was consistently > 9 pmolCy3/μgRNA. .. Single color hybridizations were carried out using the Agilent Gene Expression Hybridization Kit (p/n 5188-5242 according to the manufacturer's instructions. .. 1650 ng of cRNA was subjected to fragmentation (30 min at 60 °C) and then hybridized to 4x44K Human Whole-Genome 60-mer oligo-chips (G4112F, Agilent Technologies) in a rotary oven (10 rpm, 65C, 17 h).

Article Title: Microarray analysis reveals long non-coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy
Article Snippet: Labeled cRNA were purified using an RNeasy mini kit (cat. no. 74106; Qiagen, GmBH, Germany). .. Each slide was hybridized with 600 ng Cy3-labeled cRNA using Gene Expression Hybridization kit (cat. no. 5188-5242; Agilent Technologies, Inc.). .. Following 17 h hybridization, slides were washed in staining dishes (cat. no. 121; Thermo Fisher Scientific, Inc.) with Gene Expression Wash Buffer kit (cat. no. 5188-5327, Agilent Technologies, Inc.).

Article Title: Co-expression network analysis of toxin-antitoxin loci in Mycobacterium tuberculosis reveals key modulators of cellular stress
Article Snippet: The microarray specifications and design have been described earlier . .. For all arrays, the Gene Expression Hybridization Kit (Agilent Technologies, USA) and recommended protocols were employed. .. Hybridization and slide processing were done according to the procedure described by Agilent Technologies.

Article Title: Overexpression of long non-coding RNA RP11-396F22.1 correlates poor prognosis of patients with early-stage cervical cancer
Article Snippet: Human cervical cancer cells were transfected with either 50 nmol si-RP11-396F22.1 or si-NC using HiPerFect Transfection (cat NO. 301705) according to the manufacturer’s instruction (QIAGEN, Germany). .. Molecular microarray was used in this procedure according to the protocol of Agilent Gene Expression Hybridization Kit. .. 10× Blocking Agent was made up to a volume of 500 μl with nuclease-free water, then votex the agent gently.

Article Title: Potential biological functions of microvesicles derived from adenoid cystic carcinoma
Article Snippet: In brief, the amplification and labeling of 500 ng of total RNA was performed according to the Agilent Low Input Quick Amp Labeling kit's protocol using Cy3 (Agilent Technologies, Inc.). .. Each slide was hybridized with 1.65 µg Cy3-labeled cRNA using a Gene Expression Hybridization kit (Agilent Technologies, Inc.) for 17 h at 50°C, according to the manufacturer's protocol. .. Following hybridization and washing with buffer (0.1X saline sodium citrate, 0.5% SDS), the processed slides were scanned with the Agilent Microarray Scanner (Agilent Technologies, Inc.).

Article Title: Genes co-amplified with ERBB2 or MET as novel potential cancer-promoting genes in gastric cancer
Article Snippet: Labeled cRNA was then purified using RNeasy® mini spin columns (Qiagen). .. Hybridization was performed using the Agilent Gene Expression Hybridization Kit; samples were hybridized by rotating at 10 rpm (65°C) for 17 h and then washed before scanning. .. Gene expression microarray slides were scanned with the microarray scanner and analyzed with FEATURE EXTRACTION v10.1.1.1.

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    Agilent technologies gene expression hybridzation kit
    Gene Expression Hybridzation Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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